CN101637264A - Oligosaccharide probiotics - Google Patents
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- CN101637264A CN101637264A CN200910013282A CN200910013282A CN101637264A CN 101637264 A CN101637264 A CN 101637264A CN 200910013282 A CN200910013282 A CN 200910013282A CN 200910013282 A CN200910013282 A CN 200910013282A CN 101637264 A CN101637264 A CN 101637264A
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Abstract
The invention relates to oligosaccharide probiotics. The invention adopts the following technical scheme: the oligosaccharide probiotics comprises 40-80 parts of pectic oligosaccarides, 40-80 parts offructo-oligosaccharides and 1-5 parts of protein by weight. Other accessories can be also added into the oligosaccharide probiotics, which are the mixtures of one or more of 5-20 parts of carbonhydrate, 0.2-2.0 parts of nitrogen nutrition components, 0.4-3.5 parts of fatty acid, 0.02-0.8 part of vitamins, 0.2-0.7 part of bioactive functional factors or 0.05-0.2 part of mineral elements. The oligosaccharide probiotics feature easy production method and easily obtained raw materials, have the antibacterial function and play a role of promoting the growth of bifidobacterium obviously.
Description
Technical field
The invention belongs to food additives and food ingredient field, relate to a kind of oligosaccharide probiotics that contains the acid compound sugar of pectin particularly with prebiotic function.
Background technology
Along with enteric microorganism is learned the development of studying, the enteric microorganism flora is illustrated gradually to the influence of host health, and the reproduction balance of enteric microorganism flora also is familiar with by people gradually to the significance of health.Probio in the enteron aisle such as Bifidobacterium, lactic acid bacteria etc. are purifying enteron aisle, are improving body nutrition and safeguard and play an important role aspect the host health.And prebiotics can improve the balance of gut flora with metabolism by optionally activating with the growth of increase enteron aisle beneficial microorganism as human body indigestion or heavy food component, thereby helps the health of human life.
The kind of prebiotics is a lot, and at present known have compound sugar, polysaccharide, plant extracts, protein hydrolysate, a polyalcohol etc., and forming commercial prebiotics mainly is some compound sugar that the bifidobacterium factor functional that rises in value is arranged.Different with general digestibility compound sugar, functional oligose as prebiotics is not digested by UGI after eating, can intactly enter large intestine, be utilized materials such as generating low molecular organic acid by the Bifidobacterium in the large intestine, the indirect health that contributes to human body in back is absorbed by the body.
At present, the compound sugar of having developed in the world has FOS, xylo-oligosaccharide, low poly lactose, isomaltose, lactosucrose, soyabean oligosaccharides and gossypose etc., has very high application performance at world's food and field of fodder.And FOS is at present human body heath care function to be studied the most deep a kind of oligosaccharides, multinational being widely used in as the food ingredient or the food additives that promote the Bifidobacterium proliferation function in the world.As typical prebiotics, its effect is confirmed by the researcher of many countries.
But above-mentioned these compound sugar all belong to the category of neutral compound sugar.Also many for the research and development of compound sugar in the world at present based on neutral compound sugar, and fewer for the research of acidic oligomer sugar.In fact, neutral compound sugar is owing to there is difficult point on the production technologies such as separation and purification difficulty in the characteristics of its chemical constitution and character.And neutral compound sugar majority only is directed to the effect of prebiotics, and function is simple, and along with the enhancing of health care consciousness, people more and more favor the health products with high-efficiency multi-function.
Summary of the invention
In order to overcome deficiency of the prior art, the invention provides the oligosaccharide probiotics that a kind of production method is simple, have high-efficiency multi-function.
To achieve these goals, the technical solution used in the present invention is: oligosaccharide probiotics, it is characterized in that comprising pectin oligosaccharide and FOS, and its weight portion proportioning is as follows: 40～80 parts of the acid compound sugar of pectin, 40～80 parts of FOSs,
Wherein, the acid compound sugar of described pectin prepares as follows:
1) getting mass percentage concentration is 1%～2% the pectin aqueous solution, and regulating pH is 2～4, at 95～120 ℃, and decomposition reaction 15min～24h; Or to get mass percentage concentration be 1%～2% the pectin aqueous solution, add pectin hydrolase or pectin lyase in the live ratio of unit of the enzyme of every gram pectin: 0.01～1.0Unit, at 30～50 ℃, pH is 2.5～4.5, decomposition reaction 30min～24h obtains pectin decomposer;
2) pectin decomposer that obtains is handled with hollow-fibre membrane or ceramic filter membrane, after reclaiming the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim the oligosaccharide mixture of molecular weight 200～6000 with NF membrane, obtaining registration with Bio-gel P-2 gel column and/or the separation of Sephadex G-25 gel filtration chromatography is 2～20 pectin oligosaccharide; Maybe the pectin decomposer that obtains is handled with hollow-fibre membrane or ceramic filter membrane, after reclaiming the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim the oligosaccharide mixture of molecular weight 200～6000 again with NF membrane, cross DEAE-Sephadex A-25 anion exchange resin, with the inorganic salt solution gradient elution of 0.05～1M of 5～10 times of suitable bed volumes, obtain registration and be 2～20 pectin oligosaccharide.
Described inorganic salts are sodium chloride, sodium phosphate, carbonic hydroammonium or natrium citricum.
Described pectin can be from plant tissue or its are squeezed the juice residue after the processing, routinely the pectin that makes of technology.The common process of preparation pectin belongs to prior art.As: can be from plant (apple, oranges and tangerines, hawthorn, tomato, eggplant etc.) in the residue after tissue or its processing of squeezing the juice by water (4～100 ℃, 3h), or acid (pH 2～6, and 4～90 ℃, 3h), or salting liquid (NaCl, EDTA, oxalates, citrate etc., 3h) wait extraction, filter, reclaim with precipitation with alcohol and obtain pectin.Also can adopt various commercially available commercial pectin.
Preferably, pectin is pectin or the commercially available extensive stock pectin that fruit extracts in hawthorn pectin, apple pectin, the oranges and tangerines plant.
Described FOS can be for commercially available; Perhaps from natural plants such as banana, carrot, asparagus, onion, witloof, potato etc., adopt prior art to extract and get; Perhaps adopt prior art, from natural plants, extract poly-polysaccharide of fruit such as inulin after, induce by the method for chemistry or biological zymetology to get; Perhaps utilize sucrose to make for raw material transforms by known method.
Above-mentioned oligosaccharide probiotics can also comprise 1～5 part of protein.
In order better to reach in the human body, the equilibrium of various nutriments, above-mentioned oligosaccharide probiotics, can also add other auxiliary material, described auxiliary material is: the mixing of one or more of 5～20 parts of carbohydrate, 1.0～2.0 parts of nitrogenous class nutrition compositions, 1.4～3.5 parts of aliphatic acid, 0.3～0.8 part of vitamin, 0.2～0.7 part of bioactive functions factor or 0.05～0.2 part of mineral element.
Described carbohydrate is glucose, sucrose, starch or cellulosic one or more mixing.
The mixing of one or more that described nitrogenous nutrition composition is lysine, nucleotides or immunoglobulin (Ig).
Described aliphatic acid is one or both the mixing of arachidonic acid or DHA.
Described vitamin is one or more mixing of vitamin A, vitamin B1, vitamin B2, vitamin B6, cobalamin, vitamin C, vitamin D, vitamin E, vitamin K, nicotinic acid, folic acid or biotin.
The mixing of one or more that the described bioactive functions factor is taurine, choline or inositol.
Described mineral element is one or more mixing of calcium, magnesium, potassium, zinc, iron, copper or iodine.
Oligosaccharide probiotics of the present invention can be a solid forms according to purposes and the needs of guaranteeing the quality, and also can be flow morphology.Can be processed into powder, tablet, capsule or oral liquid as required.
Oligosaccharide probiotics of the present invention can join sweet milk, and is edible together in dairy products or other food.To dilute edible being advisable about 10 times.Recommending daily intaking amount is 5-10g/ days.
The invention has the beneficial effects as follows:
One, after the pectin oligosaccharide produced as stated above of the present invention and amphibious bacillus are cultivated altogether, can significantly promote the growth of amphibious bacillus under isolated condition.Feed and significantly to promote the propagation of amphibious bacillus in the mouse intestinal behind the mouse.Zhi Bei pectin oligosaccharide has the effect of good promotion enteron aisle Bifidobacterium Bifidum propagation as stated above, can be used as the good material of enteron aisle prebiotics.
Two, by the pectin oligosaccharide of method of the present invention preparation except that having the performance low in calories that general compound sugar has, antibiotic (the E1-Nakeebet al. that also has some neutral compound sugar and do not had, Plant Medica, 18,201-209.1970), get rid of noxious material (Inoue K et al., Journal of the Japan Society of Waste ManagementExperts such as the interior heavy metal element of human body, 13,216-222,2002), promote absorption (the Innami et al. of human body to calcium ion, Dietary Fiber, Dai-Ichi syuppan, p25,1982) etc. multiple function.
Three, the acid compound sugar of pectin has the cheap and abundant characteristics of raw material, and the ion characteristic that it is unique makes its easy separation, thereby on producing, preparation easily.
Four, the pectin oligosaccharide produced as stated above of the present invention mixes the oligosaccharide probiotics that the back is formed with FOS, than with under acid compound sugar of the pectin under the isoconcentration or the independent operating position of FOS, the obvious synergistic facilitation is played in the growth of amphibious bacillus.
Description of drawings
Fig. 1 is the influence of pectin oligosaccharide to bifidobacterium growth;
Fig. 2 is the influence of pectin oligosaccharide to mouse intestinal bifidobacterium growth amount;
Fig. 3 is the influence of oligosaccharide probiotics to mouse intestinal bifidobacterium growth amount.
The specific embodiment
Raw material: FOS adopts commercial FOS.
(1) preparation of pectin oligosaccharide:
1) with Fructus Crataegi or the residue after squeezing the juice by water (50～100 ℃), extracted 3 hours, pectin recovery method is routinely used precipitation with alcohol, reclaim hawthorn pectin.
2) getting mass percentage concentration is 1% the hawthorn pectin aqueous solution, and regulating pH is 3, and at 110 ℃, decomposition reaction 3h obtains the hawthorn pectin analyte;
3) the hawthorn pectin analyte that obtains is handled with hollow-fibre membrane, after reclaiming the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim molecular weight 200～6000 daltonian oligosaccharide mixtures with NF membrane, separate with Sephadex G-25 gel column with Bio-gel P-2 that to obtain registration be 2～20 pectin oligosaccharide.
(2) preparation of oligosaccharide probiotics
Oligosaccharide probiotics, form by weight ratio by following raw material:
Pectin oligosaccharide 60g, FOS 60g,
After above-mentioned raw materials mixed, technology was made electuary routinely.
Embodiment 2
(1) preparation of the acid compound sugar of pectin:
1) with Fructus Crataegi or the residue after squeezing the juice by water (50～100 ℃), extracted 3 hours, pectin recovery method is routinely used precipitation with alcohol, reclaim hawthorn pectin.
2) getting mass percentage concentration is 1% the hawthorn pectin aqueous solution, and regulating pH is 3, and at 110 ℃, decomposition reaction 3h obtains the hawthorn pectin analyte;
3) the hawthorn pectin analyte that obtains is handled with hollow-fibre membrane, after reclaiming the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim molecular weight 200～6000 daltonian oligosaccharide mixtures with NF membrane, separate with Sephadex G-25 gel column with Bio-gel P-2 that to obtain registration be 2～20 pectin oligosaccharide.
(2) preparation of oligosaccharide probiotics
Oligosaccharide probiotics, form by weight ratio by following raw material:
Pectin oligosaccharide 80g, FOS 40g, protein 5g,
After above-mentioned raw materials mixed, technology was made electuary routinely.Get oligosaccharide probiotics, concentration by 0.5% joins in the GAM nutrient solution, adds a certain amount of activated Bifidobacterium, after 37 ℃ of anaerobism are cultivated 24h, at the 660nm place absorbance of nutrient solution is detected, the result shows that the growth to Bifidobacterium has obvious facilitation.
Embodiment 3
(1) preparation of the acid compound sugar of pectin:
1) apple is squeezed the juice after the processing, the residue of squeezing the juice extracts after 3 hours by oxalic acid solution (pH 2～6,50～90 ℃), filters, and filtrate pectin recovery method is routinely used precipitation with alcohol, and recovery obtains apple pectin.
2) getting mass percentage concentration is 2% the apple pectin aqueous solution, and regulating pH is 2, and at 95 ℃, decomposition reaction 24h obtains the apple pectin analyte;
3) the apple pectin analyte that obtains is handled with ceramic filtration membrane, reclaim the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim molecular weight 200～6000 daltonian oligosaccharide mixtures with NF membrane, obtaining registration with the separation of Sephadex G-25 gel filtration chromatography is 2～20 pectin oligosaccharide.
(2) preparation of oligosaccharide probiotics
Oligosaccharide probiotics, form by weight ratio by following raw material:
Pectin oligosaccharide 40g, FOS 80g, protein 1g, glucose 10g,
After above-mentioned raw materials mixed, technology was made tablet routinely.Method by embodiment 2 detects, and the result shows that the growth to Bifidobacterium has obvious facilitation.
Embodiment 4
(1) preparation of pectin oligosaccharide:
1) the citrus leather bag is passed through hydrochloric acid (pH 2～6,50～90 ℃), extract after 3 hours, filter, filtrate pectin recovery method is routinely used precipitation with alcohol, reclaims and obtains citrus pectin.
2) getting mass percentage concentration is 1% the citrus pectin aqueous solution, and regulating pH is 4, and at 120 ℃, decomposition reaction 15min obtains the citrus pectin analyte;
3) the citrus pectin analyte that obtains is carried out hyperfiltration treatment with hollow-fibre membrane, after reclaiming the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim molecular weight 200～6000 daltonian oligosaccharide mixtures with NF membrane, separate with Sephadex G-25 gel filtration chromatography with Bio-gel P-2 that to obtain registration be 2～20 pectin oligosaccharide.
(2) preparation of oligosaccharide probiotics
Oligosaccharide probiotics, form by weight ratio by following raw material:
Pectin oligosaccharide 60g, FOS 50g, protein 3g, sucrose 5g, lysine 0.5g, vitamin A 6mg, vitamin B1 100mg, calcium 80mg, potassium 10mg,
After above-mentioned raw materials mixed, technology was made capsule routinely.Method by embodiment 2 detects, and the result shows that the growth to Bifidobacterium has obvious facilitation.
Embodiment 5
(1) pectin oligosaccharide preparation:
1) getting mass percentage concentration is 1% pectin (commercial pectin) solution, by every gram pectin:
0.1Unit the live ratio of unit of enzyme add pectin hydrolase, at 40 ℃, pH is 3.5, decomposition reaction 3h obtains pectin decomposer;
2) pectin decomposer that obtains is handled with ceramic filter membrane, after reclaiming the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim the oligosaccharide mixture of molecular weight 200～6000 again with NF membrane, cross DEAE-Sephadex A-25 anion exchange resin, with the sodium-chloride water solution gradient elution of the 0.08M of 8 times of suitable bed volumes, obtain registration and be 2～20 pectin oligosaccharide.
(2) preparation of oligosaccharide probiotics
Oligosaccharide probiotics, form by weight ratio by following raw material:
Pectin oligosaccharide 50g, FOS 50g, protein 3g, starch 20g, nucleotides 0.3g, DHA 1.0g, vitamin B1 80mg, vitamin D 60 μ g, vitamin E 50mg, nicotinic acid 35mg, calcium 100mg, potassium 10mg,
After above-mentioned raw materials mixed, technology was made capsule routinely.Method by embodiment 2 detects, and the result shows that the growth to Bifidobacterium has obvious facilitation.
Embodiment 6
(1) preparation of pectin oligosaccharide:
1) getting mass percentage concentration is 2% pectin (commercial) solution, by every gram pectin:
0.01Unit the live ratio of unit of enzyme add pectin lyase, at 50 ℃, pH is 2.5, decomposition reaction 24h obtains pectin decomposer;
2) described pectin decomposer is carried out hyperfiltration treatment with hollow-fibre membrane, after reclaiming the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim molecular weight 200～6000 daltonian oligosaccharide mixtures with NF membrane, cross DEAE-Sephadex A-25 anion exchange resin, with the sodium phosphate aqueous solution gradient elution of the 0.05M of 10 times of suitable bed volumes, obtain registration and be 2～20 pectin oligosaccharide.
(2) preparation of oligosaccharide probiotics
Oligosaccharide probiotics, form by weight ratio by following raw material:
Pectin oligosaccharide 70g, FOS 50g, protein 4g, glucose 16g, immunoglobulin (Ig) 500mg, arachidonic acid 2g, vitamin A 6mg, vitamin B6 20mg, cobalamin 20 μ g, vitamin E 60mg, taurine 0.3g, choline 0.5mg, calcium 100mg, potassium 10mg, zinc 15mg.
After above-mentioned raw materials mixed, technology was made oral liquid routinely.Method by embodiment 2 detects, and the result shows that the growth to Bifidobacterium has obvious facilitation.
Embodiment 7
(1) preparation of pectin oligosaccharide:
1) getting mass percentage concentration is 1% pectin solution, adds pectin hydrolase in the live ratio of unit of the enzyme of every gram pectin: 1.0Unit, and at 30 ℃, pH is 4.5, and decomposition reaction 30min obtains pectin decomposer;
2) described pectin decomposer is carried out hyperfiltration treatment with hollow-fibre membrane, after reclaiming the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim molecular weight 200～6000 daltonian oligosaccharide mixtures with NF membrane, cross DEAE-Sephadex A-25 anion exchange resin, with the ammonium bicarbonate aqueous solution gradient elution of the 1M of 5 times of suitable bed volumes, obtain registration and be 2～20 pectin oligosaccharide.
(2) preparation of oligosaccharide probiotics
Oligosaccharide probiotics, form by weight ratio by following raw material:
Pectin oligosaccharide 70g, FOS 50g, protein 4g, glucose 16g, immunoglobulin (Ig) 500mg, vitamin A 6mg, vitamin B6 20mg, taurine 0.3g, choline 0.5mg, calcium 100mg, potassium 10mg, zinc 15mg, iron 30mg.
After above-mentioned raw materials mixed, technology was made electuary routinely.Method by embodiment 2 detects, and the result shows that the growth to Bifidobacterium has obvious facilitation.
Embodiment 8
(1) preparation of pectin oligosaccharide:
1) getting mass percentage concentration is 1% the pectin aqueous solution, and regulating pH is 3, and at 110 ℃, decomposition reaction 3h obtains pectin decomposer;
2) described pectin decomposer is handled with ceramic filter membrane, after reclaiming the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim the oligosaccharide mixture of molecular weight 200～6000 again with NF membrane, cross DEAE-Sephadex A-25 anion exchange resin, with the sodium citrate aqueous solution gradient elution of the 0.06M of 8 times of suitable bed volumes, obtain registration and be 2～20 pectin oligosaccharide.
(2) preparation of oligosaccharide probiotics
Oligosaccharide probiotics, form by weight ratio by following raw material:
Fruit compound sugar 55g; FOS 55g; protein 3g; glucose 10g; lysine 0.6g; nucleotides 0.3g; immunoglobulin (Ig) 500mg; arachidonic acid 1.5g; DHA1.0g; vitamin A 6mg; vitamin B1 60mg; vitamin B2 30mg; vitamin B6 25mg; cobalamin 20 μ g; vitamin C 400mg; vitamin D 60 μ g; vitamin E 40mg; vitamin K 500 μ g; biotin 300 μ g; nicotinic acid 35mg; folic acid 0.5mg; taurine 0.2g; choline 0.Smg; inositol 200mg; calcium 60mg; magnesium 7mg; potassium 6mg; zinc 15mg; iron 30mg; copper 4mg; iodine 1.5mg.
After above-mentioned raw materials mixed, technology was made electuary routinely.Method by embodiment 2 detects, and the result shows that the growth to Bifidobacterium has obvious facilitation.
The acid compound sugar of embodiment 9 pectin is to the facilitation of the amphibious bacillus reproduction of probio
The acid compound sugar of pectin of getting embodiment 1 preparation experimentizes.
Adopt no glucose GAM culture medium, 37 ℃ are carried out the anaerobism cultivation Bifidobacterium are activated.Activated Bifidobacterium connects bacterium by the volume of equivalent and bacteria concentration and goes into contrast and contain in the culture medium of the acid compound sugar of certain density pectin, after 37 ℃ of anaerobism are cultivated 24h, in the absorbance of 660nm place detection nutrient solution.With the nutrient solution that do not add the acid compound sugar of pectin in contrast, investigate the variation of its absorbance, judge the facilitation of the acid compound sugar of pectin bifidobacterium growth with this.If promptly the absorbance of the acid compound sugar nutrient solution of pectin is higher than contrast, represent that its growth to Bifidobacterium has facilitation.As shown in Figure 1, acid compound sugar of pectin and Bifidobacterium after the co-incubation, the viable count of its reproductive growth is significantly more than the viable count that does not add pectin oligosaccharide under isolated condition.
Embodiment 10 pectin oligosaccharides are to the facilitation of the amphibious bacillus reproduction of probio:
The pectin oligosaccharide of getting embodiment 1 preparation experimentizes.
(body weight 20～25g) is randomized into basal feed group and pectin oligosaccharide processed group by body weight to cleaning level male mice in kunming in 6 age in week, 10 every group.The compound sugar processed group begins to irritate stomach (150mg/kg.bw) pectin oligosaccharide in the experiment first day, and control group is irritated the distilled water of stomach and compound sugar processed group corresponding volume.Once a day, continuous 30 days.Behind last filling stomach, water 24h is can't help in fasting.Dissect, the ight soil of getting small intestine stage casing part separates cultivates (BBL culture medium: peptone 15g/L; Dusty yeast 2g/L; Glucose 20g/L; Soluble starch 0.5g/L; NaCl5g/L:5% cysteine 10ml/L; Tween-80 0.1ml/L; Liver extract 80ml/L; Tomato leachate 400ml/L) Bifidobacterium is wherein investigated the situation of change of its clump count.As shown in Figure 2, corresponding to the experimental result under the above-mentioned isolated condition, the acid compound sugar of pectin can increase probio---the quantity of Bifidobacterium in the mouse intestinal equally significantly, plays the effect of regulating intestinal colony.
Adopt the oligosaccharide probiotics of embodiment 1 preparation to experimentize.
Adopt no glucose GAM culture medium, 37 ℃ are carried out the anaerobism cultivation, Bifidobacterium are activated.Activated Bifidobacterium connects bacterium and goes into to contain respectively in the culture medium that concentration expressed in percentage by weight is 0.5% pectin oligosaccharide, FOS and oligosaccharide probiotics of the present invention, and 37 ℃ are carried out anaerobism.After cultivating 24h, take a sample,, detect the wherein growth population of amphibious bacillus by dull and stereotyped multiple dilution cultivation with reference to the counting method of GB GB4789.2-2003 total number of bacteria.As shown in Figure 3, oligosaccharide probiotics of the present invention has the effect of obvious synergistic effect to the growth of Bifidobacterium, that is to say, pectin oligosaccharide and FOS can be used as the good combination of prebiotics with important physiological function.
Claims (4)
1, oligosaccharide probiotics is characterized in that comprising pectin oligosaccharide and FOS, and its weight portion proportioning is as follows: 40～80 parts of pectin oligosaccharides, 40～80 parts of FOSs,
Wherein, described pectin oligosaccharide prepares as follows:
1) getting mass percentage concentration is 1%～2% the pectin aqueous solution, and regulating pH is 2～4, at 95～120 ℃, and decomposition reaction 15min～24h; Or to get mass percentage concentration be 1%～2% the pectin aqueous solution, add pectin hydrolase or pectin lyase in the live ratio of unit of the enzyme of every gram pectin: 0.01～1.0Unit, at 30～50 ℃, pH is 2.5～4.5, decomposition reaction 30min～24h obtains pectin decomposer;
2) pectin decomposer that obtains is handled with hollow-fibre membrane or ceramic filter membrane, after reclaiming the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim the oligosaccharide mixture of molecular weight 200～6000 with NF membrane, obtaining registration with Bio-gel P-2 gel column and/or the separation of Sephadex G-25 gel filtration chromatography is 2～20 pectin oligosaccharide; Maybe the pectin decomposer that obtains is handled with hollow-fibre membrane or ceramic filter membrane, after reclaiming the following oligosaccharide mixture of molecular weight 6000 dalton, reclaim the oligosaccharide mixture of molecular weight 200～6000 again with NF membrane, cross DEAE-Sephadex A-25 anion exchange resin, with the inorganic salt solution gradient elution of 0.05～1M of 5～10 times of suitable bed volumes, obtain registration and be 2～20 pectin oligosaccharide;
Described inorganic salts are sodium chloride, sodium phosphate, carbonic hydroammonium or natrium citricum.
2,, it is characterized in that comprising 1～5 part of protein according to the described oligosaccharide probiotics of claim 1.
3, according to the described oligosaccharide probiotics of claim 2, it is characterized in that comprising auxiliary material, described auxiliary material is: the mixing of one or more of 5～20 parts of carbohydrate, 0.2～2.0 part of nitrogenous class nutrition composition, 0.4～3.5 part of aliphatic acid, 0.02～0.8 part of vitamin, 0.20～0.70 part of bioactive functions factor or 0.05～0.20 part of mineral element.
4, according to the described oligosaccharide probiotics of claim 3, it is characterized in that:
Described carbohydrate is glucose, sucrose, starch or cellulosic one or more mixing;
The mixing of one or more that described nitrogenous class nutrition composition is lysine, nucleotides or immunoglobulin (Ig);
Described aliphatic acid is one or both the mixing of arachidonic acid or DHA;
Described vitamin is one or more mixing of vitamin A, vitamin B1, vitamin B2, vitamin B6, cobalamin, vitamin C, vitamin D, vitamin E, vitamin K, nicotinic acid, folic acid or biotin;
The mixing of one or more that the described bioactive functions factor is taurine, choline or inositol.
Described mineral element is one or more mixing of calcium, magnesium, potassium, zinc, iron, copper or iodine.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102342396A (en) * | 2011-04-09 | 2012-02-08 | 常德市城头山绿色饮料有限公司 | Oral liquid containing xylitol zinc calcium |
| CN106857837A (en) * | 2017-02-13 | 2017-06-20 | 安徽宇宁果胶股份有限公司 | A kind of probiotics and prebiotics composite beverage |
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| CN103142640A (en) * | 2013-04-01 | 2013-06-12 | 李拖平 | Health care product containing vitamin K2 and having functions of preventing and treating brittle-bone disease |
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| DE602004020809D1 (en) * | 2003-10-24 | 2009-06-04 | Nutricia Nv | Immunomodulatory oligosaccharides |
| CN101153304A (en) * | 2007-09-21 | 2008-04-02 | 天津农学院 | Acidity oligosaccharide of pectin and its use |
| CN101153307A (en) * | 2007-09-21 | 2008-04-02 | 天津农学院 | Method of producing acidity oligosaccharide of pectin |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN102342396A (en) * | 2011-04-09 | 2012-02-08 | 常德市城头山绿色饮料有限公司 | Oral liquid containing xylitol zinc calcium |
| CN102342396B (en) * | 2011-04-09 | 2013-06-12 | 常德市城头山绿色饮料有限公司 | Oral liquid containing xylitol zinc calcium |
| CN106857837A (en) * | 2017-02-13 | 2017-06-20 | 安徽宇宁果胶股份有限公司 | A kind of probiotics and prebiotics composite beverage |
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