CN101627722A - Homozygous method for genetic male sterile line of brassica napus - Google Patents
Homozygous method for genetic male sterile line of brassica napus Download PDFInfo
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- CN101627722A CN101627722A CN200810029609A CN200810029609A CN101627722A CN 101627722 A CN101627722 A CN 101627722A CN 200810029609 A CN200810029609 A CN 200810029609A CN 200810029609 A CN200810029609 A CN 200810029609A CN 101627722 A CN101627722 A CN 101627722A
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- brassica napus
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Abstract
The invention provides a homozygous method for a genetic male sterile line of brassica napus. The sowing area and the sowing period of seeds of the genetic male sterile line of the brassica napus are adjusted to make the brassica napus blossom under the condition that the average daily temperature at the blossoming stage of the brassica napus is between 19 and 25 DEG C; the fertile plants are pulled out before blossoming, and the sterile plants are kept; partial sterile plants are converted into the fertile or semi-sterile plants at the average daily temperature of between 19 and 25 DEG C; the converted fertile or semi-sterile plants are put into bags for self-breeding to obtain the self-breeding seed; the brassica napus is homozygous by repeating the steps through one or several generations; the brassica napus is sowed under the condition that the average daily temperature at the blossoming stage of the upland cotton is less than 19 DEG C, and the sterility of the sterile plants is evaluated. By the method, the sterile plant rate of the genetic male sterile line of the brassica napus is improved from nearly 50 percent to 100 percent, so that the method has the advantages of saving the step of pulling out the fertile plants in the process of the seed production by breeding, simplifying the seed production procedure, improving the yield of the seed production, reducing the seed cost and improving the seed impurity.
Description
Technical field
The invention belongs to a kind of homozygous method for genetic of male sterile line of brassica napus.
Background technology
Produce crossbreed with male sterile line of brassica napus, must respectively pull out the educated strain that accounts for sum about 50% in male-sterile seed breeding field and seed farm respectively, this not only makes seed farm output reduce, and the crossbreeding process is numerous and diverse, is difficult to guarantee seed purity.
Summary of the invention
The homozygous method for genetic that the purpose of this invention is to provide a kind of male sterile line of brassica napus, make the sterile plant rate of male sterile line of brassica napus rise to 100% by 50%, thereby exempt the crossbreeding process and pull out the operation that to educate strain, simplify production of hybrid seeds program, improve hybrid seed yield, reduce seed costs, improve seed purity.
The present invention regulates the sowing ground and the sowing time of male sterile line of brassica napus seed, being allowed to condition at the flowering stage daily mean temperature is that 19 ℃-25 ℃ condition is bloomed, pull out before blooming and to educate strain, stay sterile strain, the partial sterility strain is to become under 19 ℃-25 ℃ the condition can educate or half sterile at daily mean temperature, to become and to educate or the selfing of semisterile sterile strain bagging, the results selfed seed, flowering stage daily mean temperature sow under being condition below 19 ℃, identify sterile strain, so continuous one to counting generation, until isozygotying.
The present invention makes the sterile plant rate of male sterile line of brassica napus rise to 100% by about 50%, pulls out the operation that can educate strain thereby exempt the crossbreeding process, simplifies production of hybrid seeds program, improves hybrid seed yield, reduces seed costs, improves seed purity.
Embodiment
Principle of the present invention is: in the sterile group of hill body of male sterile line of brassica napus, some is to become male-fertile or half sterile by male sterile under 19 ℃-25 ℃ the condition at daily mean temperature, allow these sterile strains under 19 ℃ of-25 ℃ of conditions, bloom, just can selfing isozygoty, become sterile plant rate and be 100% male sterile line.
Embodiment 1
Cabbage type rape 117AB pulls out before blooming and can educate strain in the autumn sowing of Zhanjiang, Guangdong, and the partial sterility strain becomes partly sterile, the bagging selfing, and the results selfed seed so isozygotys after continuous 5 generations, and sterile plant rate is identified in the Changsha autumn sowing.The sterile plant rate of 117AB rises to 100% by present 50%, has exempted the crossbreeding process and has pulled out the operation that can educate strain, and hybrid seed yield improves 30%, and seed costs reduces by 40%.
Embodiment 2
Loudi, cabbage type rape 98-116AB Hunan winter sowing, bloom April, pulls out before blooming and can educate strain, and the partial sterility strain becomes partly sterile, the bagging selfing, the results selfed seed so isozygotys after continuous 5 generations, and sterile plant rate is identified in the Loudi autumn sowing.The sterile plant rate of 98-116AB rises to 100% by present 50%, has exempted the crossbreeding process and has pulled out the operation that can educate strain, and hybrid seed yield improves 30%, and seed costs reduces by 40%.
Claims (1)
1, a kind of homozygous method for genetic of male sterile line of brassica napus, it is characterized in that regulating the sowing ground and the sowing time of male sterile line of brassica napus seed, being allowed to condition at the flowering stage daily mean temperature is that 19 ℃-25 ℃ condition is bloomed, pull out before blooming and to educate strain, stay sterile strain, the partial sterility strain is to become under 19 ℃-25 ℃ the condition can educate or half sterile at daily mean temperature, to become and to educate or the selfing of semisterile sterile strain bagging, the results selfed seed, so continuous one to counting generation, until isozygotying, flowering stage daily mean temperature be to sow under 19 ℃-25 ℃ the condition, identify sterile plant rate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810029609A CN101627722A (en) | 2008-07-16 | 2008-07-16 | Homozygous method for genetic male sterile line of brassica napus |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810029609A CN101627722A (en) | 2008-07-16 | 2008-07-16 | Homozygous method for genetic male sterile line of brassica napus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101627722A true CN101627722A (en) | 2010-01-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN200810029609A Pending CN101627722A (en) | 2008-07-16 | 2008-07-16 | Homozygous method for genetic male sterile line of brassica napus |
Country Status (1)
| Country | Link |
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| CN (1) | CN101627722A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638447A (en) * | 2016-03-29 | 2016-06-08 | 湖北利众种业科技有限公司 | Breeding method for increasing rape nuclear sterile line yield and improving seed pureness |
-
2008
- 2008-07-16 CN CN200810029609A patent/CN101627722A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105638447A (en) * | 2016-03-29 | 2016-06-08 | 湖北利众种业科技有限公司 | Breeding method for increasing rape nuclear sterile line yield and improving seed pureness |
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| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
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| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20100120 |