The specific embodiment
The present invention is further illustrated below in conjunction with specific embodiment.
The following example can make those skilled in the art more fully understand the present invention, but does not limit the present invention in any way.
Embodiment 1
The application of deoxyschizandrin in the sun-proof ointment of preparation:
Deoxyschizandrin 10 grams, Borneolum Syntheticum 0.5 gram.
(glyceryl monostearate 20 restrains ointment base fat, octadecanol 5 restrains, stearic acid 10 restrains, vaseline 10 restrains, glycerol 10 restrains, triethanolamine 2 restrains, sodium lauryl sulphate 2 restrains, antiseptic is an amount of, essence is an amount of, deionized water adds to 100 and restrains in right amount; )
Glyceryl monostearate, stearic acid, vaseline, octadecanol are mixed and heated to 75 ℃ are oil phase.In addition glycerol, triethanolamine and appropriate amount of deionized water are heated to 75 ℃, add sodium lauryl sulphate again and be dissolved as water.Then water is slowly poured in the oil phase, the limit edged stirs, and until cooling, promptly gets basic fat.Deoxyschizandrin and Borneolum Syntheticum are added in the basic fat, stir, add antiseptic, spice promptly.
Embodiment 2
The application in the sun-proof ointment of preparation of deoxyschizandrin and schisandrin B:
Deoxyschizandrin 6g, schisandrin B 4g, menthol 0.5g, ointment base fat are an amount of, and (glyceryl monostearate 60g, vaseline 40g, hexadecanol 30g, liquid wax 4g, glycerol 70g, S-60 are an amount of, T-80 is an amount of, azone is an amount of, antiseptic is an amount of, essence is an amount of; )
Glyceryl monostearate, vaseline, hexadecanol, liquid wax, S-60 are put into beaker together mix, heating in water bath is to 80-90 ℃ of fusion; Deoxyschizandrin, schisandrin B add sneaks into oil phase after an amount of ethanol dissolves fully, waves the ethanol that looses to there not being the alcohol flavor;
Glycerol, T-80 add in the Purified Water, are heated to 70-80 ℃; Under continuous stirring condition, water slowly is added in the oil phase, stop to heat, when being cooled to 60 ℃ of left and right sides, under constantly stirring, adding azone, antiseptic continuation stirring, adding menthol and essence stirred evenly when temperature was reduced to 40-50 ℃, promptly.
Embodiment 3
The application of deoxyschizandrin in the sun-proof ointment of preparation:
Deoxyschizandrin 3g, Borneolum Syntheticum 0.5g, ointment base fat are an amount of, and (glyceryl monostearate 60g, vaseline 40g, hexadecanol 30g, liquid wax 4g, glycerol 70g, S-60 are an amount of, T-80 is an amount of, azone is an amount of, antiseptic is an amount of, essence is an amount of; )
Glyceryl monostearate, deoxyschizandrin, vaseline, hexadecanol, liquid wax, S-60 are put into beaker together mix, heating in water bath is to 80-90 ℃ of fusion; Add and sneak into oil phase after an amount of ethanol dissolves fully, wave the ethanol that looses to there not being the alcohol flavor.
Glycerol, T-80 add in the Purified Water, are heated to 70-80 ℃; Under continuous stirring condition, water slowly is added in the oil phase, stop to heat, when being cooled to 60 ℃ of left and right sides, under constantly stirring, adding azone, antiseptic continuation stirring, adding Borneolum Syntheticum and essence stirred evenly when temperature was reduced to 40-50 ℃, promptly.
Embodiment 4
Deoxyschizandrin and schisandrin B are in the application in the sun-proof Emulsion of preparation
Each 10g of deoxyschizandrin and schisandrin B, arabic gum 10.0g, tragakanta 5.0g, 5% ethyl hydroxybenzoate 6mL, liquid Paraffin 50.0mL, purified water adds to 1000mL.
Deoxyschizandrin and schisandrin B, arabic gum, tragakanta are put in the mortar and ground well, add aqueous paraffin and grind well, add purified water 25mL fast, acutely be ground to rapidly and send splitting sound, get colostrum, dilute with purified water then.5% ethyl hydroxybenzoate is dissolved in an amount of hot purified water, mixes with colostric fluid then, add purified water at last, stir evenly promptly to 1000mL.
Embodiment 5
Deoxyschizandrin, schisandrin B, the application of schisantherin A in the sun-proof Emulsion of preparation:
Each 8g of deoxyschizandrin and schisandrin B, schisantherin A 5g, arabic gum 10.0g, tragakanta 5.0g, 5% ethyl hydroxybenzoate 6mL, liquid Paraffin 50.0mL, purified water adds to 1000mL.
Deoxyschizandrin, schisandrin B, schisantherin A, arabic gum, tragakanta are put in the mortar and ground well, add aqueous paraffin and grind well, add purified water 25mL fast, acutely be ground to rapidly and send splitting sound, get colostrum, dilute with purified water then.5% ethyl hydroxybenzoate is dissolved in an amount of hot purified water, mixes with colostric fluid then, add purified water at last, stir evenly promptly to 1000mL.
Embodiment 6
The application of schisantherin A in the sun-proof membrane of preparation.
Schisantherin A 5 grams, EPO 5g, isopropyl alcohol 30ml, laurocapram 2ml adds ethanol to 100ml.
Get schisantherin A, add isopropyl alcohol, ethanol, stir, put in 50 ℃ of tepidariums, stir and make dissolving.Other gets EPO, adds ethanol, stirs, make dissolving after, add in the above-mentioned solution, stir evenly, add laurocapram, add ethanol at last to full dose, promptly.
Embodiment 7
The application of SALLRSIPA SAL ADNF-9 schizronol schisanhenol in the agent of preparation sun protection gel.
SALLRSIPA SAL ADNF-9 schizronol schisanhenol 5g, card pool nurse 1g, glycerol 5g, ethanol 20ml, propylene glycol 10ml, triethanol ammonium is an amount of, and purified water adds to 100ml.
Card taking pool nurse is sprinkled into and makes its slow swelling in an amount of purified water, adds glycerol, propylene glycol, stirs evenly, and slowly adds triethanol ammonium, and the limit edged stirs and makes into clear gel base fat.SALLRSIPA SAL ADNF-9 schizronol schisanhenol is dissolved in the ethanol, is added in the gel base fat, add purified water then to capacity, stir evenly evenly, packing promptly.
The pharmacological results
Lignanoids is to the absorption and the protective effect of ultraviolet injury in the Fructus Schisandrae Chinensis
1. to the in vitro study of ultraviolet radiation injury protection
The uv absorption rate: adopt solwution method, obtain solution at first, getting ethanol is 1: 1 than tri-distilled water volume ratio, and medicine is added in the solution, is made into mass concentration and is 10% mother solution, when measuring, with 20 times of mensuration of mother solution dilution.Adopt ultraviolet spectrophotometer to measure absorption value, in strict accordance with experimental working technique.We choose every 5nm and one increase gradient, and data record is from 250nm~400nm, and experimental result is observed and found that selected active ingredient of Chinese herbs can obviously resist the UVA in the ultraviolet, and UVB (sees Fig. 1-Fig. 3).
Experimental technique: get HaCat (the biochemical epidermis of the people's immortality) cell of growth logarithmic (log) phase, 0.25% trypsinization gets 0.5 * 10
5The cell of cells/ml is layered on 96 orifice plates, and every hole adds 100 μ L cells, cultivated 1 day, after adopting serum-free medium to make up a prescription, at first, the reject first wife supports base, every hole adds dosing culture medium 100 μ L, cultivated 1 day, and went culture medium then, after PBS cleaning 2 times, add fresh PBS100 μ L again, use 45mJ/cm
2Dosage UVB irradiation HaCat cell removes PBS, adds fresh serum-free medium, behind the cultivation 4h, adds MTT, behind the effect 4h, at the 490nm place, measures its survival rate with microplate reader, finds our selected compounds, has tangible uvioresistant activity.What the concentration of experiment Chinese medicine was chosen respectively is 1 * 10
-5~1 * 10
-7Mol/L.
2. zoopery
Experimental technique: 25 of rats are divided into 5 groups at random, every group 5,5 groups give 5 kinds of medicines respectively at the back, at first, each is organized every back of rat and plucks by 4cm * 4cm, the part of plucking is equally divided into A, B, C, D four parts, and the A part is coating not, and B, C, D part are respectively by behind mass concentration 2.5%, 5.0%, the 7.5% coating effect 2h, follow vertical irradiation in rats back 30min under uviol lamp, apart from lamp 20cm, after irradiation stops 4h, erythema partly occurs with A and be advisable.The choosing part of plucking at the back is again done blank for E, and size is with 2*2cm
2Be advisable.Get A, B, C, D, E five part skins, do tissue slice, He dyeing, under optical microscope (* 400), observe the fibroblast number in the dermal tissue unit are, and under micrometer, measure epidermal thickness.
The ointment compound method is seen embodiment 1.
4. irritation test
Select 3 of healthy rabbits, body weight is 2.5-3.0kg, and both sides, every rabbit back select a place to shave a mao 2.5cm
2, use the own control test method(s), will contain sunscreen cream and blank each 0.5g of basic fat of medicine respectively, be coated in equably and shave a mao position, 2 times/d, dermoreaction behind observation and record 24h and the 72h, reactions such as that the result does not all have is rubescent, dermexanthesis, blister illustrate this ointment nonirritant, external safety.
Effect and ultraviolet infringement that lignanoids natural products such as target compound deoxyschizandrin of the present invention, schisandrin B, schisandrin C, schisandrin, schisantherin B, Fructus Schisandrae Chinensis alcohol ester first, SALLRSIPA SAL ADNF-9 schizronol schisanhenol have ultra-violet absorption have defencive function.The advantages of nontoxic raw materials that this product is used, low price, with low cost, safe and efficient, market prospect is wide, and the economic benefit and the social benefit of expection are considerable.
Used effective ingredient and effective site have commercially availablely among the present invention, or can reach in accordance with known methods to describe in the document and prepare.