CN101609000B - Optical fiber evanescent wave biomembrane activity detection sensor - Google Patents

Abstract

An optical fiber evanescent wave biomembrane activity detection sensor comprises incident optical fiber, emergent optical fiber, a collimating mirror and a focusing mirror, and is characterized in that: the sensor is internally provided with a first fixed mirror, a first movable mirror, a second movable mirror and a prism; a mounting hole is arranged in the bottom center of the sensor, the prism is arranged in the mounting hole on the sensor bottom, and the bottom of the prism protrudes outwardly; the upper portion of the prism is a cuboid, a front side and a back side of the lower portion of the prism take the shape of an isosceles trapezoid with an upper base line longer than a lower base line, and a left side and a right side of the lower portion of the prism take a shape of a rectangle; a reflecting surface of the first fixed mirror forms an angle of 45 degrees with the center line of the incident optical fiber, the first movable mirror forms an angle of beta degrees with a horizontal plane, and the reflecting surface of the first movable mirror simultaneously corresponds to the reflecting surface of the first fixed mirror and a side of the prism. The sensor can perform online detection of biomembrane activity and has advantages of accurate detection, rapid response and long service life.

Description

A kind of optical fiber evanescent wave biomembrane activity detection sensor

Technical field

The present invention relates to Fibre Optical Sensor, be specifically related to a kind of optical fiber evanescent wave biomembrane activity detection sensor.

Background technology

Handle in the process of low-concentration organic exhaust gas (VOCs) at bio-trickling filter, because the biochemical reaction process of biological exhaust-gas treatment is very complicated, the factor that influences exhaust-gas treatment efficient is a lot, as contain temperature etc. in the composition, biomass concentration, pH value, rate of circulation circulating fluid flow rate, tower of the bacteria suspension of microorganism and nutriment, will finally influence the biomembrane activity directly related in above-mentioned each factor with the organic exhaust gas degradation rate.Literature research confirms that intracellular a lot of vital vital movement processes all are present in cell surface, and the secretion of cell surface has confidential relation with the vital movement of cell again.Therefore this problem proposes to measure the reaction biomembrane activity by detection of biological film exocellular polysaccharide biological membrane exocytosis thing, and wherein the biological membrane exocellular polysaccharide mainly comprises protein, nucleic acid, lipid etc.

Existing literature research shows, the clean-up effect effect of biological treatment depends mainly on biomass and biologically active, and biofilm biomass or biological membrane thickness all can only reflect the number of the biomass of apposition growth microorganism, and can not reflect its activity, therefore need to pass judgment on suspension growth and apposition growth activation of microorganism by means of other indexs.

Being used for the biomembrane activity detection method at present mainly is the off-line measurement method, and the off-line measurement method mainly contains: measure its dehydrogenase activity method, atriphos in the biological membrane (ATP) content method, oxygen consumption rate method.It is not only time-consuming but also require great effort that these methods are measured biomembrane activity, most importantly, no matter be to measure dehydrogenase activity or measure Adenosine triphosphate battalion (ATP) content, all need biological membrane from adhering to carrier such as filling ball is taken off, in this process, biological membrane exocytosis thing and cell will sustain damage, so this method is difficult to realize that real biomembrane activity detects.Very easily bring simultaneously assorted bacterium into and infect reaction tower in sampling process, another shortcoming that adopts off-line method is to be difficult for implementing robotization control.Though the oxygen consumption rate method need not split away off biological membrane to measure from adhering to carrier such as filling ball, how accurate online detection oxygen consumption rate (OCR) is not resolved as yet.Deliver about the up to the present also relevant both at home and abroad research paper of online detection method.

Though have certain methods can measure biomembrane activity, also be in the comparatively original stage.Because be engaged in the researchers of this research field, normally dehydrogenasa and TTC reaction are generated TF, the activity that the TF color is deeply felt bright dehydrogenasa more is high more.Thereby be used for rough judgement biomembrane activity.

The green technology of handling low-concentration organic exhaust gas at bioanalysis enters engineering after the application stage, how to measure biomembrane activity in industrial biochemistry reaction treatment process, improves the exhaust gas decomposition rate, is technical barrier and key that present this technology enters the engineering application stage.

In sum, system uses fast travelling waves of optical fibre (evanescent wave, be abbreviated as: EW) energy attenuation technology realizes the biomembrane activity on-line measurement, output data by sensor, demonstration biomembrane activity situation of change constantly, thereby allow the researchers that are engaged in this research field, recognize the growth and breeding situation of biological bacteria liquid, thereby add the information of the aspects such as activity of nutrient solution and observation biological bacteria timely for biological bacteria from microcosmic.

At this technical barrier and key, by research to the physical phenomenon of EW, designed a kind of new E W biomembrane activity survey sensor, and, drawn and received the energy of light and the funtcional relationship of biological membrane exocellular polysaccharide refractive index by theoretical analysis to Design of Sensor principle and method, light path.On this basis, furtherd investigate the influence to biomembrane activity under the light sources with different wavelengths situation of biological membrane exocellular polysaccharide, experimental result and theoretical analysis show: it is feasible that this method is used to measure biomembrane activity, have advantages such as biomass concentration is measured accurately, is quick on the draw, long service life, this Design of Sensor principle and method have certain universal significance, be a kind of very big practical biological film activity sensor that has, have a good application prospect.

Summary of the invention

At prior art exist can not the on-line measurement biomembrane activity defective, technical matters to be solved by this invention provides a kind of optical fiber evanescent wave biomembrane activity detecting line sensor.

In order to solve the problems of the technologies described above, according to technical scheme of the present invention, a kind of optical fiber evanescent wave biomembrane activity detecting line sensor, comprise incident optical, outgoing optical fiber, collimating mirror, focus lamp is characterized in: be provided with first fixed mirror, first index glass, second index glass, second fixed mirror, prism in sensor; Centre in sensor base has mounting hole, prism is arranged in the mounting hole of sensor base, and outwards protrude the bottom surface of prism, the top of prism is rectangular parallelepiped, and the pro and con of the bottom of prism is shaped as upper base, and to be longer than the left surface and the right flank of the bottom of the isosceles trapezoid of going to the bottom, prism be rectangle; The reflecting surface of described first fixed mirror becomes miter angle with the center line of incident optical, and first index glass becomes β degree angle with surface level, and the reflecting surface of first index glass is simultaneously corresponding with a side of the reflecting surface of first fixed mirror, prism; Described collimating mirror receives the incident ray that incident optical sends, make light all focus on back formation parallel rays and be transmitted to first fixed mirror, first fixed mirror receives the light of collimating mirror emission, and light reflected to first index glass, first index glass reflects light to a side of prism, and another side of prism reflects light to second index glass; The reflecting surface of second fixed mirror becomes miter angle with the center line of outgoing optical fiber, second index glass becomes φ degree angle with surface level, and the reflecting surface of second index glass is simultaneously corresponding with another side of the reflecting surface of second fixed mirror, prism, second index glass reflects light to second fixed mirror, second fixed mirror reflects light to focus lamp, and focus lamp focuses on the back and launches by outgoing optical fiber.

According to a preferred version of the present invention, also be provided with mount pad at the top of sensor, in sensor, also be provided with mounting bracket, two mounting blocks, two and adjust screw rod, spring, wherein, described mounting bracket is fixed on the mount pad; Described mounting blocks is arranged on the lower end of mounting bracket respectively by installation shaft, described mounting blocks is provided with the inclined-plane, the inclined-plane of one of them mounting blocks is simultaneously corresponding with a side of first fixed mirror, prism, and the inclined-plane of another mounting blocks is simultaneously corresponding with another side of second fixed mirror, prism; Spring is arranged between two mounting blocks; One of them is adjusted screw rod and is arranged between the top and mount pad of one of them mounting blocks, and another is adjusted screw rod and is arranged between the top and mount pad of another mounting blocks, adjusts screw rod for two and is fixed on the mount pad by threaded hole respectively; First index glass and second index glass stick on respectively on the inclined-plane of two mounting blocks.

According to a preferred version of the present invention, described mounting bracket is made of connecting link and base, and wherein, base is worker's shape, is made of an outrigger and two crossbeams, and an end of connecting link is fixed on the outrigger of base, and the other end of connecting link is fixed on the mount pad; Described mounting blocks is arranged in the groove between two crossbeams of base by installation shaft respectively.

According to a preferred version of the present invention, the right flank of described prism and the angle theta of sensor base are:

θ＝arcsin(n ₂/n ₁)

Wherein: n ₁Be the refractive index of prism, n ₂Be organic refractive index to be measured.

According to a preferred version of the present invention, the angle β of first index glass and surface level is:

$\frac{1}{2}\arcsin (n_2/n_1)\≤\mathrm{\β}\≤2\arcsin (n_2/n_1)$

Wherein: n ₁Be the refractive index of prism, n ₂Be organic refractive index to be measured.

According to a preferred version of the present invention, described prism adopts silicon crystal to constitute.

The beneficial effect of a kind of optical fiber evanescent wave biomembrane activity detecting line sensor of the present invention is: this invention can be carried out the online detection of biomembrane activity, have detect accurately, be quick on the draw, the advantage of long service life, be a kind of very big practical biological film activity sensor that has, have a good application prospect.

Description of drawings

Below in conjunction with accompanying drawing the present invention is elaborated.

Fig. 1 is an optical fiber evanescent wave biomembrane activity detecting line sensor structural representation of the present invention.

Fig. 2 is the structural representation of prism 7 of the present invention.

Fig. 3 is the total reflection synoptic diagram of light in prism 7.

Fig. 4 is an evanescent wave image synoptic diagram of the present invention.

Fig. 5 is the light path synoptic diagram of first index glass 6 of the present invention under first extremum conditions.

Fig. 6 is the light path synoptic diagram of first index glass 6 of the present invention under the secondary extremal condition.

Fig. 7 is the mounting structure synoptic diagram of first index glass 6 of the present invention, second index glass 8.

Embodiment

Referring to Fig. 1 to Fig. 7, a kind of optical fiber evanescent wave biomembrane activity detecting line sensor, by sensor outer housing, incident optical 1, outgoing optical fiber 2, collimating mirror 3, focus lamp 4, first fixed mirror 5, first index glass 6, second index glass 8, second fixed mirror 9, prism 7, mount pad 17, mounting bracket 11, two adjust screw rod 12,13, installation shaft 14,15, spring 16, two mounting blocks 18,19, supporting seats 20,21 are formed.Wherein, have mounting hole 10 in the centre position of sensor base, prism 7 is arranged in the mounting hole of sensor base, and outwards protrude the bottom surface, the top of prism 7 is rectangular parallelepiped, and the pro and con of the bottom of prism 7 is shaped as upper base, and to be longer than the left surface and the right flank of the bottom of the isosceles trapezoid of going to the bottom, prism 7 be rectangle; Described first fixed mirror 5 and second fixed mirror 9 are fixed in the shell wall of sensor by supporting seat 20,21, the reflecting surface of described first fixed mirror 5 becomes miter angle with the center line of incident optical 1, and the reflecting surface of second fixed mirror 9 becomes miter angle with the center line of outgoing optical fiber 2; The reflecting surface while of first index glass 6 is corresponding with a side of the reflecting surface of first fixed mirror 5, prism 7; Mount pad 17 is arranged on the top of sensor, and described mounting bracket 11 is fixed on the mount pad 17; Described mounting blocks 18,19 is arranged on the lower end of mounting bracket 11 respectively by installation shaft 14,15; Described mounting bracket 11 is made of connecting link 22 and base 23, and wherein, base 23 is worker's shapes, is made of an outrigger and two crossbeams, and an end of connecting link 22 is fixed on the outrigger of base 23, and the other end of connecting link 22 is fixed on the mount pad 17; Described mounting blocks 18,19 is arranged in the groove between two crossbeams of base by installation shaft 14,15 respectively; Described mounting blocks 18,19 is provided with the inclined-plane, the inclined-plane of mounting blocks 18 is simultaneously corresponding with a side of first fixed mirror 5, prism 7, and the inclined-plane of mounting blocks 18 becomes β degree angle with surface level, the inclined-plane of mounting blocks 19 is simultaneously corresponding with another side of second fixed mirror 9, prism 7, and the inclined-plane of mounting blocks 19 becomes φ degree angle with surface level; Spring 16 is arranged between the mounting blocks 18,19; Adjust screw rod 12 and be arranged between the top and mount pad 17 of mounting blocks 18, adjust screw rod 13 and be arranged between the top and mount pad 17 of mounting blocks 19, adjust screw rod 12,13 and be fixed on the mount pad 17 by threaded hole respectively; First index glass 6 and second index glass 8 stick on respectively on the inclined-plane of mounting blocks 18,19, can adjust screw rod 12,13 with the screwdriver rotation, finish the adjustment of mounting blocks slight distance about in the of 18,19, be used to adjust angle β, the φ of index glass 6,8 and surface level, spring 16 is used to produce equilibrant, makes mounting blocks 18,19 keep balance.

Described collimating mirror 3 receives the incident ray that incident optical 1 sends, and makes light all focus on back formation parallel rays and is transmitted to first fixed mirror 5; First fixed mirror 5 reflects light to first index glass 6, first index glass 6 reflects light to a side of prism 7, after in prism 7, passing through repeatedly total reflection, another side of prism 7 reflects light to second index glass 8, second index glass 8 reflects light to second fixed mirror 9, second fixed mirror 9 reflects light to focus lamp 4, after focus lamp 4 focuses on, launches by outgoing optical fiber 2; Wherein, the catoptrical side of reception first index glass 6 of described prism 7 and the angle theta of sensor base are:

θ＝arcsin(n ₂/n ₁)

Wherein: n ₁Be the refractive index of prism 7, n ₂Be organic refractive index to be measured.

First index glass 6 with the angle β of surface level is:

$\frac{1}{2}\arcsin (n_2/n_1)\≤\mathrm{\β}\≤2\arcsin (n_2/n_1);$

Second index glass 8 with the angle of regulation range of the included angle of surface level is:

$\frac{1}{2}\arcsin (n_2/n_1)\≤\mathrm{\φ}\≤2\arcsin (n_2/n_1).$

Described prism 7 material selection refractive indexes are 3.4 Si crystal.

The catoptrical side of reception first index glass 6 of described prism 7 and the angle theta angle of sensor base are chosen and are based on:

Incident light reflexes on the side of prism 7, promptly satisfy total reflection condition, as shown in Figure 3, Figure 4, know by geometrical optics in the side, if incident light satisfies total reflection condition in the side of prism 7, can satisfy on prism 7 He on the bottom surface and satisfy total reflection condition.

The organism refractive index requires promptly that incident angle can satisfy total reflection condition on whole organism index distribution interval in measurement generally between 1.0-1.5.

By total reflection formula sin θ=n ₂/ n ₁Know that wherein, θ is the critical angle of incidence when total reflection takes place, n ₁Be the refractive index of Si crystal, n ₂Be organic refractive index to be measured, when choosing the organism refractive index n ₂=1.5 o'clock, n ₁=3.4, substitution formula θ=arcsin (n ₂/ n ₁): incident angle is θ=26.17897 °, can satisfy the total reflection condition between whole measurement zone.

The adjustable extent of index glass angle adopts extremum method to determine:

As shown in Figure 5, when the angle beta of first index glass 6 and surface level=2 θ, the incident angle of incident light this moment in the side of prism 7 is 90 °, promptly impinges perpendicularly on the side of prism 7, and do not have light to arrive second index glass 8 this moment.

As shown in Figure 6, when light beam directly reflexes to the bottom surface of prism 7 through first index glass 6, light beam behind the bottom reflection of prism 7, parallel arrival second index glass 8 of light beam with the another side of prism 7, at this moment:

Know that from above two extreme points first index glass 6 with the angle of regulation range of the angle β of surface level is:

For making second index glass 8 can receive light beam, the angle of regulation range of the included angle of second index glass 8 and surface level is for also being designed to: Expression formula θ=arcsin (n by θ ₂/ n ₁) know that first index glass 6 with the angle of regulation range of the angle β of surface level is:

$\frac{1}{2}\arcsin (n_2/n_1)\≤\mathrm{\β}\≤2\arcsin (n_2/n_1);$

Second index glass 8 with the angle of regulation range of the included angle of surface level is:

$\frac{1}{2}\arcsin (n_2/n_1)\≤\mathrm{\φ}\≤2\arcsin (n_2/n_1);$

Biomembrane activity Fundamentals of Sensors based on optical fiber EW evanescent wave energy attenuation quantitative changeization

The relation of I/O light intensity when the light beam process is received medium

Intensity law when light beam is propagated in weak absorbing medium can get the strength relationship of bright dipping between 2 of weak absorbing medium and is:

I _Out=I _Ine ^{-α l}(1)

α=nK wherein ₀K, n are biological membrane exocellular polysaccharide refractive index to be measured, K ₀, K is specific constant.

If absorbing light intensity changes with position change.At this moment (one) formula will be extended to following formula:

$I_{\mathrm{out}}=I_{\mathrm{in}}\exp (-{\∫}_A^B\mathrm{\α}\left(r\right)\mathrm{dl})$ (2)

From origin-to-destination, (two) formula often is called Bill Beer law to integration along selected light in the formula.The relation of EW energy attenuation amount and biological membrane exocellular polysaccharide concentration

When EW entered the biological membrane exocellular polysaccharide, its penetration depth depended on the refractive index of refractive index, biological membrane exocytosis thing of incident light wavelength, the used crystal of sensor and the light incident angle at used crystal interface.Penetration depth D is calculated by following formula:

$D=\frac{\mathrm{\λ}}{2\mathrm{\π}{n}_1{[{\sin }^2\mathrm{\θ}-{(n_2/n_1)}^2]}^{1/2}}$ (3)

In the formula, λ is a lambda1-wavelength; n ₁Refractive index for the used crystal of sensor; n ₂Be thing film exocellular polysaccharide refractive index; θ is an incident angle.

Supposition now, the EW light intensity is I ⁱ, the light intensity of light direct reflected back behind the interface is I _i, then have:

I _In=I ⁱ+ I _i(4)

With (C) formula substitution (A) Shi Kede, output intensity behind the EW process thing film exocellular polysaccharide:

$I_{\mathrm{out}}^{\′}=I^i\exp (-\frac{n_2{K}_0\mathrm{K\λ}}{2\mathrm{\π}{n}_1{\left[{\sin }^2\mathrm{\θ}{-(n_2/n_1)}^2\right]}^{1/2}})$ (5)

Know the EW off-energy by (5) formula, promptly thing film exocellular polysaccharide is to the absorption energy of incident light wave:

I _Absorb=I ⁱ-I ' _Out(6)

This sensor simultaneously according to specifically required, designs the size of sensing probe in order to improve the sensitivity of detection.As the order of reflection of light in the Si crystal is N.

By (six) formula as can be known, when with the order of reflection being N calculating, and supposition equates that at the energy of each reflection spot EW loss then the incident light total energy loss is:

I _Loss=N (I ⁱ-I ' _Out)+I " (seven)

Wherein I " is that the Si crystal is to the absorption of incident light energy.

Know that by (seven) formula final output intensity is:

$I_{\mathrm{out}}=I_{\mathrm{in}}-N[I^i-I^i\exp (-\frac{n_2{K}_0\mathrm{K\λ}}{2\mathrm{\π}{n}_1{[{\sin }^2\mathrm{\θ}-{(n_2/n_1)}^2]}^{1/2}})]-I^{\′\′}$ (8)

Further supposition, the light intensity of light direct reflected back behind the interface is I _iWith evanescent wave light intensity I ⁱRatio be constant K, know:

I _In=KI ⁱ+ I ⁱ=(K+1) I ⁱ=K ₁I ⁱ(9)

After the distortion of (nine) formula substitution (eight) formula, have:

$I_{\mathrm{out}}=I_{\mathrm{in}}-\frac{{\mathrm{NI}}_{\mathrm{in}}}{K_1}[1-\exp (-\frac{n_2{K}_0\mathrm{K\λ}}{2\mathrm{\π}{n}_1{[{\sin }^2\mathrm{\θ}-{(n_2/n_1)}^2]}^{1/2}})]-\frac{I_{\mathrm{in}}}{K_2}$ (10)

That is:

$I_{\mathrm{out}}=I_{\mathrm{in}}[(1-\frac{N}{K_1}-\frac{1}{K_2})+\frac{N}{K_1}\exp (-\frac{n_2{K}_0\mathrm{K\λ}}{{2\mathrm{\πn}}_1{[{\sin }^2\mathrm{\θ}-{(n_2/n_1)}^2]}^{1/2}})]$ (11)

Wherein $I^{\′\′}=\frac{I_{\mathrm{in}}}{K_2}.$

Order $K_4=1-\frac{N}{K_1}-\frac{1}{K_2},$ $K_5=\frac{N}{K_1},$ ${\mathrm{<figure-callout\; id="6"\; label="K"\; filenames="H2009101044082E00031.png"\; state="\{\{state\}\}">K</figure-callout>}}_6=\frac{K_0\mathrm{K\&lambda;}}{2{\mathrm{\&pi;n}}_1},$ Then (11) formula is reduced to:

$I_{\mathrm{out}}=I_{\mathrm{in}}[K_4+K_5\exp (-\frac{n_2{K}_6}{[{{\sin }^2\mathrm{\&theta;}-{(n_2/n_1)}^2]}^{1/2}})]$ (12)

Represent the output voltage of light reflected light after opto-electronic conversion with U.K ₇The photoelectric conversion factors of the expression photo-detector D then output voltage after opto-electronic conversion is:

$U=K_4{K}_7{I}_{\mathrm{in}}[1+\frac{K_5}{K_4}\exp (-\frac{n_2{K}_6}{{[{\sin }^2\mathrm{\&theta;}-{(n_2/n_1)}^2]}^{1/2}})]$ (13)

That is:

$\mathrm{Ln}(\frac{K_{4}U}{K_4{K}_5{K}_7{I}_{\mathrm{in}}}-\frac{K_4}{K_5})=-\frac{n_2{K}_6}{{[{\sin }^2\mathrm{\&theta;}-{(n_2/n_1)}^2]}^{1/2}}$ (14)

Because K _i(i=1,2,3,4,5,6,7) are constant, then can make: ${\mathrm{<figure-callout\; id="8"\; label="K"\; filenames="H2009101044082E00031.png"\; state="\{\{state\}\}">K</figure-callout>}}_8=\frac{K_4}{K_4{K}_5{K}_7{I}_{\mathrm{in}}},$ $K_9=\frac{K_4}{K_5},$ Then have:

${n_2}^2=n_1^2{\sin }^2\mathrm{\&theta;}+\frac{n_1^4{\sin }^2\mathrm{\&theta;}{\mathrm{<figure-callout\; id="6"\; label="K"\; filenames="H2009101044082E00031.png"\; state="\{\{state\}\}">K</figure-callout>}}_6^2}{{\mathrm{Ln}}^2(K_{8}U-K_9)-n_1^2{\mathrm{<figure-callout\; id="6"\; label="K"\; filenames="H2009101044082E00031.png"\; state="\{\{state\}\}">K</figure-callout>}}_6^2}$ (15)

That is:

${n_2}^2={(B_1+\frac{B_3}{{\mathrm{Ln}}^2(K_{8}U-K_9){-B}_2})}^{1/2}$ (16)

Wherein $B_1=n_1^2{\sin }^2\mathrm{\&theta;},$ $B_2=n_1^2{\mathrm{<figure-callout\; id="6"\; label="K"\; filenames="H2009101044082E00031.png"\; state="\{\{state\}\}">K</figure-callout>}}_6^2,$ $B_3=n_1^4{\sin }^2{\mathrm{\&theta;<figure-callout\; id="6"\; label="K"\; filenames="H2009101044082E00031.png"\; state="\{\{state\}\}">K</figure-callout>}}_6^2.$

Pass between sensor output voltage and the biological membrane exocellular polysaccharide concentration is:

$U=\frac{1}{{\mathrm{<figure-callout\; id="8"\; label="K"\; filenames="H2009101044082E00031.png"\; state="\{\{state\}\}">K</figure-callout>}}_8}\exp {(B_2+\frac{B_3}{n_2^2-B_1})}^{1/2}+\frac{K_9}{K_8}$ (17)

From (17) formula as can be seen, the input light intensity I that sends by launching fiber of EW biomembrane activity detecting line sensor _InBehind silicon crystal Si, at silicon crystal Si and biological membrane exocellular polysaccharide boundary reflection, the latent ripple of vowing enters generation decay in the biological membrane exocellular polysaccharide, reflected light reaches reception optical fiber behind silicon crystal Si, photo-detector photoelectricity conversion back (conversion coefficient is K) becomes voltage signal U again, carries out data processing through being input to computing machine after signal condition, the A/D conversion, promptly knows the size of biomembrane activity.

Claims (6)

1. optical fiber evanescent wave biomembrane activity detecting line sensor, comprise incident optical (1), outgoing optical fiber (2), collimating mirror (3), focus lamp (4) is characterized in that: be provided with first fixed mirror (5), first index glass (6), second index glass (8), second fixed mirror (9), prism (7) in sensor; Have mounting hole (10) in the centre of sensor base, prism (7) is arranged in the mounting hole (10) of sensor base, and outwards protrude the bottom surface of prism (7), the top of prism (7) is rectangular parallelepiped, and the pro and con of the bottom of prism (7) is shaped as upper base, and to be longer than the left surface and the right flank of the bottom of the isosceles trapezoid of going to the bottom, prism (7) be rectangle; The reflecting surface of described first fixed mirror (5) becomes miter angle with the center line of incident optical (1), first index glass (6) becomes β degree angle with surface level, and the reflecting surface of first index glass (6) is simultaneously corresponding with a side of the reflecting surface of first fixed mirror (5), prism (7); Described collimating mirror (3) receives the incident ray that incident optical (1) sends, make light all focus on back formation parallel rays and be transmitted to first fixed mirror (5), first fixed mirror (5) receives the light of collimating mirror (3) emission, and light reflected to first index glass (6), first index glass (6) reflects light to a side of prism (7), and another side of prism (7) reflects light to second index glass (8); The reflecting surface of second fixed mirror (9) becomes miter angle with the center line of outgoing optical fiber (2), second index glass (8) becomes φ degree angle with surface level, and the reflecting surface of second index glass (8) is simultaneously corresponding with another side of the reflecting surface of second fixed mirror (9), prism (7), second index glass (8) reflects light to second fixed mirror (9), second fixed mirror (9) reflects light to focus lamp (4), and focus lamp (4) focuses on the back and launches by outgoing optical fiber (2).

2. a kind of optical fiber evanescent wave biomembrane activity detecting line sensor according to claim 1, it is characterized in that: also be provided with mount pad (17) at the top of sensor, in sensor, also be provided with mounting bracket (11), two mounting blocks (18,19), two and adjust screw rod (12,13), spring (16), wherein, described mounting bracket (11) is fixed on the mount pad (17); Described mounting blocks (18,19) is arranged on the lower end of mounting bracket (11) respectively by installation shaft (14,15), described mounting blocks (18,19) is provided with the inclined-plane, the inclined-plane of mounting blocks (18) is simultaneously corresponding with a side of first fixed mirror (5), prism (7), and the inclined-plane of mounting blocks (19) is simultaneously corresponding with another side of second fixed mirror (9), prism (7); Spring (16) is arranged between the mounting blocks (18,19); Adjusting screw rod (12) is arranged between the top and mount pad (17) of mounting blocks (18), adjust screw rod (13) and be arranged between the top and mount pad (17) of mounting blocks (19), adjust screw rod (12,13) and be fixed on the mount pad (17) by threaded hole respectively; First index glass (6) and second index glass (8) stick on respectively on the inclined-plane of mounting blocks (18,19).

3. a kind of optical fiber evanescent wave biomembrane activity detecting line sensor according to claim 2, it is characterized in that: described mounting bracket (11) is made of connecting link (22) and base (23), wherein, base (23) is worker's shape, constitute by an outrigger and two crossbeams, one end of connecting link (22) is fixed on the outrigger of base (23), and the other end of connecting link (22) is fixed on the mount pad (17); Described mounting blocks (18,19) is arranged in the groove between two crossbeams of base (23) by installation shaft (14,15) respectively.

4. according to claim 1,2 or 3 described a kind of optical fiber evanescent wave biomembrane activity detecting line sensors, it is characterized in that: the catoptrical side of reception first index glass (6) of described prism (7) and the angle theta of sensor base are:

θ＝arcsin(n ₂/n ₁)

Wherein: n ₁Be the refractive index of prism (7), n ₂Be organic refractive index to be measured.

5. a kind of optical fiber evanescent wave biomembrane activity detecting line sensor according to claim 4,

It is characterized in that: first index glass (6) with the angle β of surface level is

Second index glass (8) with the angle of regulation range of the included angle of surface level is:

6. a kind of optical fiber evanescent wave biomembrane activity detecting line sensor according to claim 5 is characterized in that: described prism (7) adopts silicon crystal to constitute.

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