CN101601967B - Four-channel full automatic gel permeation purification system for macromolecule - Google Patents
Four-channel full automatic gel permeation purification system for macromolecule Download PDFInfo
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- CN101601967B CN101601967B CN2009101580910A CN200910158091A CN101601967B CN 101601967 B CN101601967 B CN 101601967B CN 2009101580910 A CN2009101580910 A CN 2009101580910A CN 200910158091 A CN200910158091 A CN 200910158091A CN 101601967 B CN101601967 B CN 101601967B
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Abstract
The invention discloses a four-channel full automatic gel permeation purification system for macromolecule, which includes a transfusion system, a sample feeding and fraction collecting system, an improved gel purification material and an analysis purification integrated column, a cleaning device and a control operation system. The transfusion system is used for feeding solvent into the purification column in real time; the sample feeding and fraction collecting system is used for injecting sample into the purification column, and collecting the purified solution; the control operation system is used for controlling the transfusion system, the sample feeding and fraction collecting system for transfusing, sample feeding, purifying, cleaning and recovering according to a definite sequence. The system is simple has simple operation, great stability, precise positioning of the fraction transferring and feeding device, capable of sample batch processing; the analysis column is miniature, and highly efficient; analysis time is short and the cost is saved.
Description
Technical field
The present invention relates to be used for the gel infiltration cleaning system of processing environment sample, food, waste pollutant and other sample.
Background technology
Gel infiltration sample purification system is the instrument that is used to purify at the bottom of all kinds of sample copies.Sample comprises: food, biological tissue, cereal, plant and environmental sample such as soil, mud and harmful waste, development along with analytical technology, gel infiltration sample purification system will be more and more general in the application aspect the residual pre-treatment separation of China's agricultural and veterinary chemicals, the purification, especially being rich in aspect the macromolecular sample separation purifications such as fat, pigment, has tangible clean-up effect.Because its course of work complexity of gel infiltration sample purification, it is long to carry out once the needed time, is generally about 50 minutes, can expend a large amount of time if want that a plurality of samples are carried out the gel infiltration purification, can't satisfy the needs in the work.
Summary of the invention
At the problem that prior art exists, the invention provides a kind of system to a plurality of samples while gel infiltration purified treatment.It can make sample feeding, purification, fraction collection realize the automatic lot processing, greatly reduces the time of sample treatment, has improved the operating efficiency of cleaning system.
For achieving the above object, full automatic gel permeation cleaning system of the present invention, comprise transfusion system, sample introduction and fraction collection system, decontaminating column, cleaning device and control operation system, transfusion system comprises solvent memory, infusion pump, solvent memory, infusion pump and decontaminating column input link to each other, and are input in the decontaminating column by the solvent of infusion pump with the solvent memory; Sample introduction and fraction collection system comprise injector, syringe pump and mechanical arm, injector is installed on the mechanical arm, mechanical arm drives injector and moves and take a sample or collect, injector, syringe pump links to each other with decontaminating column, mechanical arm moved injector to cleaning device when injector purified the sample extraction in the test tube to decontaminating column, injector is cleaned, solution after the purification flow in the gathering-device by injector again, and the control operation system is used to control transfusion system, sample introduction and fraction collection system infuse sequentially, sample introduction, purify, clean and reclaim.
Further, the corresponding decontaminating column of described transfusion system and sample introduction and fraction collection system, transfusion system, sample introduction and fraction collection system and decontaminating column comprise one group at least.
Further, described sample introduction and fraction collection system also comprise six direction changeover valves, four-way switching valve and quantity tube, and six direction changeover valves connect the input of four-way switching valve, quantity tube, described syringe pump and described decontaminating column, and four-way switching valve connects described injector.
Further, the input of described infusion pump connects described solvent memory, and output connects described six direction changeover valves.
Further, the input of described syringe pump connects
Further, described injector is the four-way sample introduction needle.
Full automatic gel permeation cleaning system of the present invention is simple to operate, good stability, accurate positioning, can the batch process sample, greatly reduce the clarification time of gel infiltration, improved operating efficiency, and system of the present invention employing Automatic Control, human error reduced.
Description of drawings
Fig. 1 is a full automatic gel permeation cleaning system front view of the present invention;
Fig. 2 is the vertical view of full automatic gel permeation cleaning system of the present invention;
Fig. 3 is an A-A view among Fig. 1.
The specific embodiment
Below in conjunction with accompanying drawing technical scheme of the present invention is further specified:
As Figure 1-3, full automatic gel permeation cleaning system of the present invention, comprise frame 1, frame 1 is provided with brace summer 11 and operating desk, guide rail 12 is set on the brace summer 11, sample tube frame 41 is set on the operating desk, fraction collection rack for test tube 61 and rinse bath 5, on the frame 1 transfusion system is set, sample introduction and fraction collection system and decontaminating column 34, transfusion system comprises infusion pump 31 and solvent bottle 32, sample introduction and fraction collection system comprise right angle mechanical arm 21, four-way sample introduction needle 23, four-way switching valve 24, six direction changeover valves 25, quantity tube 26 and syringe pump 27, four-way sample introduction needle 23 is installed on the right angle mechanical arm 21, be connected with mechanical arm drag chain 22 on the mechanical arm 21, mechanical arm 21 is erected on the guide rail 12 and can slides on guide rail 12; The input of infusion pump 31 connects solvent bottle 32, output connects six direction changeover valves 25, six direction changeover valves 25 connect the input of four-way switching valve 24, quantity tube 26, syringe pump 27 and decontaminating column 34, four-way switching valve 24 connects four-way sample introduction needle 23, the output of decontaminating column 34 connects waste collection device 7, syringe pump 27 1 ends connect six direction changeover valves 25, and the other end connects reagent bottle 33.
Also comprise in the system of the present invention be used to control that transfusion system, sample introduction and fraction collection system infuse sequentially, the control operation system (not shown) of sample introduction, purification, cleaning and recovery.
The concrete job step of full automatic gel permeation cleaning system of the present invention is as follows: with pending sample put into test tube 42, test tube 42 is put on the sample tube frame 41 in order, fraction collection pipe 62 is put in order receive on the rack for test tube 61.By operating system editor setting program, bring into operation on demand by operational outfit.Infusion pump 31 by six direction changeover valves, 25 input decontaminating columns 34, enters waste collection device 7 from four-way switching valve 24 with the solvent in the solvent bottle 32 again, carries out balance.After right angle mechanical arm 21 moves to sample tube 42 tops, mechanical arm 21 descends and makes four-way sample introduction needle 23 2mm place to the sample liquid level, syringe pump 27 extracts, sample is passed through four-way sample introduction needle 23, four-way switching valve 24, six direction changeover valves, 25 suction syringe pumps 27, mechanical arm 21 descends with liquid level, four-way sample introduction needle 23 is in 2mm place under the liquid level all the time, inject quantity tube 26 by six direction changeover valves 25 again behind the sample suction syringe pump 27, inject decontaminating column 34 by six direction changeover valves 25 again and purify, the solvent waste liquid enters in the waste liquid receiving bottle 7 by four-way switching valve 24.Mechanical arm 21 rises simultaneously, moves to rinse bath 5 tops again, and mechanical arm 21 descends and cleans sample introduction needle 23.When being purified to setting-up time, mechanical arm 21 moves to collects test tube 62 tops, and mechanical arm 21 descends, and collects solution and flows in the fraction collection pipe 62 by four-way switching valve 24, sample introduction needle 23, carries out fraction collection.Collection finishes, and mechanical arm 21 moves to rinse bath 5 again and cleans sample introduction needle 23, and the solvent waste liquid enters in the waste liquid receiving bottle 7 by four-way switching valve 24 more simultaneously, until finishing setting program.
Full automatic gel permeation cleaning system of the present invention by the control operation system is preestablished, carries out transfusion, sample introduction, purification, cleaning and the recovery of sample fully automatically, effectively raises operating efficiency, and has reduced the influence of manual operation error.Adopt many group transfusion system, sample introduction and fraction collection system and decontaminating columns in the system of the present invention, can the batch process sample, greatly reduce the clarification time of gel infiltration, improved operating efficiency.
It is to be noted and any distortion of making according to the specific embodiment of the present invention all do not break away from the scope that spirit of the present invention and claim are put down in writing.
Claims (5)
1. four-channel full automatic gel permeation purification system for macromolecule, it is characterized in that, comprise transfusion system, sample introduction and fraction collection system, decontaminating column, cleaning device and control operation system, transfusion system comprises solvent memory, infusion pump, solvent memory, infusion pump and decontaminating column input link to each other, and are input in the decontaminating column by the solvent of infusion pump with the solvent memory; Sample introduction and fraction collection system comprise the four-way sample introduction needle, syringe pump and mechanical arm, the four-way sample introduction needle is installed on the mechanical arm, the four-way sample introduction needle that drives mechanical arm moves and takes a sample or collect, the four-way sample introduction needle, syringe pump links to each other with decontaminating column, mechanical arm moved the four-way sample introduction needle to cleaning device when the four-way sample introduction needle purified the sample extraction in the test tube to decontaminating column, the four-way sample introduction needle is cleaned, solution after the purification flow in the gathering-device by the four-way sample introduction needle again, and the control operation system is used to control transfusion system, sample introduction and fraction collection system infuse sequentially, sample introduction, purify, clean and reclaim; Can purify 2-4 sample solution or continuous purification sample 1-16 simultaneously, purification efficiency promotes significantly.
2. full automatic gel permeation purification system for macromolecule as claimed in claim 1 is characterized in that, the corresponding decontaminating column of described transfusion system and sample introduction and fraction collection system, and transfusion system, sample introduction and fraction collection system and decontaminating column comprise one group at least.
3. full automatic gel permeation purification system for macromolecule as claimed in claim 1, it is characterized in that, described sample introduction and fraction collection system also comprise six direction changeover valves, four-way switching valve and quantity tube, six direction changeover valves connect the input of four-way switching valve, quantity tube, described syringe pump and described decontaminating column, and four-way switching valve connects described four-way sample introduction needle.
4. full automatic gel permeation purification system for macromolecule as claimed in claim 3 is characterized in that, the input of described infusion pump connects described solvent memory, and output connects described six direction changeover valves.
5. full automatic gel permeation purification system for macromolecule as claimed in claim 3 is characterized in that, the input of described syringe pump connects described solvent memory, and output connects described six direction changeover valves.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101580910A CN101601967B (en) | 2009-07-20 | 2009-07-20 | Four-channel full automatic gel permeation purification system for macromolecule |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2009101580910A CN101601967B (en) | 2009-07-20 | 2009-07-20 | Four-channel full automatic gel permeation purification system for macromolecule |
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| Publication Number | Publication Date |
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| CN101601967A CN101601967A (en) | 2009-12-16 |
| CN101601967B true CN101601967B (en) | 2011-06-22 |
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| CN2009101580910A Expired - Fee Related CN101601967B (en) | 2009-07-20 | 2009-07-20 | Four-channel full automatic gel permeation purification system for macromolecule |
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Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106198162A (en) * | 2016-06-24 | 2016-12-07 | 维科托（北京）科技有限公司 | Accelerate solvent extraction gel purification combined instrument and control method thereof |
| CN109696346B (en) * | 2017-10-20 | 2023-10-13 | 莱伯泰科(天津)科技有限公司 | Isotope pretreatment device |
| CN108896375B (en) * | 2018-09-20 | 2023-12-22 | 天津屹诺维信仪器有限公司 | Full-automatic heavy metal separation enrichment extraction appearance |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1407337A (en) * | 2001-09-03 | 2003-04-02 | 株式会社资生堂 | Liquid chromatographic system, sampler, cleaning device and method |
| CN2832294Y (en) * | 2005-10-25 | 2006-11-01 | 中国农业大学 | Automatic flow path separating device |
| CN1955712A (en) * | 2005-10-25 | 2007-05-02 | 中国农业大学 | Automatic separation flow circuit device |
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2009
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1407337A (en) * | 2001-09-03 | 2003-04-02 | 株式会社资生堂 | Liquid chromatographic system, sampler, cleaning device and method |
| CN2832294Y (en) * | 2005-10-25 | 2006-11-01 | 中国农业大学 | Automatic flow path separating device |
| CN1955712A (en) * | 2005-10-25 | 2007-05-02 | 中国农业大学 | Automatic separation flow circuit device |
Non-Patent Citations (1)
| Title |
|---|
| JP特开2003-75419A 2003.03.12 |
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| CN101601967A (en) | 2009-12-16 |
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