CN101596221A - A kind of preparation technology of water-soluble ginkgo biloba extract used for injection - Google Patents
A kind of preparation technology of water-soluble ginkgo biloba extract used for injection Download PDFInfo
- Publication number
- CN101596221A CN101596221A CNA2008100385066A CN200810038506A CN101596221A CN 101596221 A CN101596221 A CN 101596221A CN A2008100385066 A CNA2008100385066 A CN A2008100385066A CN 200810038506 A CN200810038506 A CN 200810038506A CN 101596221 A CN101596221 A CN 101596221A
- Authority
- CN
- China
- Prior art keywords
- ginkgo
- extract
- content
- total
- hours
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Abstract
The invention discloses the preparation technology of the water soluble ginkgo leaf extract that a kind of ejection preparation uses.Comprise preparation 0.1%～0.3% pharmagel aqueous solution, dissolve Folium Ginkgo extract, ultrafiltration, vacuum drying with aqueous gelatin solution and prepare ginkgo terpene total lactones and preparation Ginkgo total flavones extract, and then carry out total mixed finished product according to separately content.Ginkgo total flavones alcohol glycosides content in the extract of the present invention: ginkgo terpene total lactones content is 4: 1, can be used as water-solubility ginkgo ejection preparation class raw material, has enlarged the scope of application, and has guaranteed the safe, effective, stable, controlled of product quality.
Description
Technical field:
The present invention relates to Chinese medicine preparation technology, be specifically related to the preparation technology of the water soluble ginkgo leaf extract that a kind of ejection preparation uses.
Background technology:
Folium Ginkgo extract is a kind of Chinese medicine extract with unique pharmacologically active that extracts from Folium Ginkgo, because its unique pharmacologically active and huge and clinical value widely, has become a focus and emphasis of present plant amedica research and development.Play the two big class materials that have of main therapeutical effect in Folium Ginkgo extract, a class is a ginkgetin class material, and a class is a bilobalide-like substances.Show after deliberation: gingkgo flavonoids has blood vessel dilating, improve the circulation of blood vessel tip and cerebrovascular, effects such as cholesterol reducing, releasing smooth muscle spasm, lax bronchus are usually used in treating with angiocardiopathy preventing and because the cerebrovascular blood supply insufficiency causes the sequela that dizziness, headache, tinnitus, hypomnesis, intelligence decline, hearing impairment, cerebrovascular accident damage cause etc.; Modern pharmacological research shows that bilobalide is the medicine that potential applicability in clinical practice is arranged in present natural antiplatelet activity factor (PAF) receptor antagonist most, and wherein the selectivity of ginkalide B antagonism paf receptor and activity are the strongest.Bilobalide can anticoagulant, and blood viscosity lowering improves ischemia patient's microcirculation, reduces thrombosis; Stabilizing cell membrane reduces the infiltration of angiotensin simultaneously; Have antiallergic, antiinflammatory, Antishock function, the rejection of ischemic injuries and organ transplantation is also had protective effect.So, at present, both at home and abroad all the quality control index of this two constituents as Folium Ginkgo extract, regulation in one one of " Chinese Pharmacopoeia 2005 " version: in the Folium Ginkgo extract content of Ginkgo total flavones alcohol glycosides should 〉=24%, content 〉=6% of ginkgo terpene total lactones.Yet, because the difference of Folium Ginkgo extract extraction process, make the Folium Ginkgo extract inherent quality exist evident difference, mainly show: content ratio, the tannin of dissolubility, terpene lactone each components contents, Ginkgo total flavones and the ginkgo terpene total lactones of Folium Ginkgo extract in water between the two is residual, resin residue etc.Have a strong impact on the Folium Ginkgo extract curative effect and the safety of the various Semen Ginkgo ejection preparations that are development of raw materials.Therefore, exist the Folium Ginkgo extract of above-mentioned mass defect can not be directly used in the Semen Ginkgo ejection preparation, must through processing make it instructions for use that quality meets injection after, just can be applied to the Semen Ginkgo ejection preparation.
Summary of the invention:
Technical problem to be solved by this invention is to overcome above-mentioned weak point, and the preparation technology of research design Folium Ginkgo extract makes the extract that obtains can meet the requirement that is used for the water solublity ejection preparation.
The invention provides the preparation technology of a kind of injection with water soluble ginkgo leaf extract.
Owing to must not contain tannin in the injection Folium Ginkgo extract, resin, and all contain above-mentioned substance in the commercially available Folium Ginkgo extract, so can not be directly used in injection ginkgo leaf extract preparation raw material, must handle before use, conventional treatment method is: adopt acid, the method of alkali is carried out tannin, the removal of resin, but this method can cause the decomposition of Ginkgo total flavones and part Semen Ginkgo terpene lactone (particularly bilobalide), influence the quality of Folium Ginkgo extract, and operating procedure is complicated, and the pharmagel aqueous solution of 40～100 times of amounts 0.10%～0.3% of process using of the present invention, Folium Ginkgo extract to commercially available different quality dissolves, can remove tannin and resin in the Folium Ginkgo extract, overcome above-mentioned defective effectively.
The injection water soluble ginkgo leaf extract requires to have good water-solubility, therefore, must remove the macromolecular substances in the Folium Ginkgo extract, guarantees injection Folium Ginkgo extract aqueous solution good stable.The ultrafiltration of this process using has well solved this problem.
In addition, the ginkgo agent product that has has certain content requirement to alcohol glycosides of the Ginkgo total flavones in the Folium Ginkgo extract and ginkgo terpene total lactones, and the two has proper proportion in Folium Ginkgo extract, otherwise can influence its use.Separating of process using Ginkgo total flavones of the present invention and ginkgo terpene total lactones, can carry out different proportionings according to different product requirements, thereby satisfy different demands, also guaranteed stable, the homogeneous, controlled of ginkgo agent product quality.
A kind of injection provided by the invention preparation technology of water soluble ginkgo leaf extract, this technology comprises the following steps:
(1) preparation 0.1%～0.3% pharmagel aqueous solution g/v: in pharmagel: purified water is 0.1～0.3: 100 ratio w/v, pharmagel slowly adds under stirring and is heated in advance in about 40 ℃ purified water, insulation, stirring all are dissolved into uniform solution until gelatin and are promptly got 0.1%～0.3% pharmagel aqueous solution g/v, and be standby;
(2) dissolving Folium Ginkgo extract: Folium Ginkgo extract adds in 0.1%～0.3% pharmagel aqueous solution, heats to 80 ℃～100 ℃, and insulated and stirred was extracted 30 minutes～60 minutes, the solution natural cooling, leave standstill, sedimentation 24 hours～36 hours, solution filters, insoluble matter discards;
(3) ultrafiltration: filtrate is used the ultrafilter membrane ultrafiltration, collect ultrafiltrate, ultrafiltrate 60 ℃～80 ℃ ,-0.085～-carry out concentrating under reduced pressure under the condition of 0.1Mpa, be concentrated to the raffinate scale of construction and be 1/16 of former ultrafiltrate volume～1/24 o'clock, stop to concentrate, concentrated solution cold preservation crystallize 24 hours～36 hours under 5 ℃～10 ℃ conditions, filter, keep filtrate, filter cake cold water washing 2～4 times keep washing liquid;
(4) vacuum drying prepares ginkgo terpene total lactones: step (3) filter cake places the vacuum drying oven drying under reduced pressure, baking temperature: 60 ℃～80 ℃, vacuum :-0.1MPa, obtains the off-white color ginkgo terpene total lactones at 3～4 hours drying times;
(5) preparation Ginkgo total flavones extract
A: concentrating under reduced pressure, decolouring: the cleaning mixture of step (3) and filtrate merge, at 60 ℃～80 ℃,-0.085～-condition of 0.1Mpa under concentrating under reduced pressure become the fluid extract relative density: 1.200～1.300,95% ethanol that in fluid extract, adds 30～50 times of amounts of Folium Ginkgo extract inventory, heating, 60 ℃～80 ℃ Wen Rong, active substance fully is dissolved in 95% alcoholic solution, to the active carbon that wherein adds 0.25%～0.5%W/V, insulation, stirred 30 minutes, solution cold preservation under 5 ℃～10 ℃ conditions is left standstill, sedimentation 24 hours～36 hours, filter, solids discards, and filtrate is stand-by;
B: with above-mentioned filtrate 60 ℃～80 ℃ ,-0.085～-carry out concentrating under reduced pressure under the condition of 0.1Mpa and become the fluid extract relative density: 1.200～1.300, fluid extract is placed the vacuum drying oven drying under reduced pressure, baking temperature: 60 ℃～80 ℃, vacuum :-0.1MPa, 3～4 hours drying times, dry thing carries out the pulverizing of 80 orders and obtains faint yellow Ginkgo total flavones extract;
(6) preparation finished product: the Ginkgo total flavones extract that the ginkgo terpene total lactones that above-mentioned steps (4) obtains and step (5) obtain is according to " method of 2005 editions one relevant Folium Ginkgo extract prescription regulation of Chinese pharmacopoeia detects the content of ginkgo terpene total lactones and Ginkgo total flavones alcohol glycosides respectively, by Ginkgo total flavones alcohol glycosides content: ginkgo terpene total lactones content is that 4: 1 content ratio is carried out total mixing, promptly obtains the water soluble ginkgo leaf extract that ejection preparation is used.
The consumption of the pharmagel aqueous solution of step of the present invention (2) described 0.1%～0.3% is 40 times～100 times W/V of Folium Ginkgo extract weight.Folium Ginkgo extract can commercially availablely obtain.
The described aqueous solution filtrate of step of the present invention (3) is that 3000～10000 ultrafilter membrane carries out ultrafiltration with molecular cut off.
The present invention adopts special production technology that the Ginkgo total flavones in the Folium Ginkgo extract is separated with ginkgo terpene total lactones, and then carry out always mixed according to content separately, promptly can obtain Ginkgo total flavones alcohol glycosides content: ginkgo terpene total lactones content is a kind of ejection preparation Folium Ginkgo extract of 4: 1, and tannin, resin meet the instructions for use of injection raw material in this extract, product has good water-solubility, can be used as water-solubility ginkgo ejection preparation class raw material.
Injection water soluble ginkgo leaf extract through prepared of the present invention has good water-solubility, and can guarantee Ginkgo total flavones alcohol glycosides content in the Semen Ginkgo extrac: ginkgo terpene total lactones content is 4: 1, reduced the difference of Folium Ginkgo extract inherent quality, enlarge the scope of application, and guaranteed the safe, effective, stable, controlled of product quality.
Description of drawings
Ginkgo total flavones alcohol glycosides assay HPLC collection of illustrative plates (contrast) in Fig. 1 Ginkgo total flavones extract
Ginkgo total flavones alcohol glycosides assay HPLC collection of illustrative plates (example 1 sample) in Fig. 2 Ginkgo total flavones extract
Fig. 3 Ginkgo total lactones assay HPLC collection of illustrative plates (contrast)
Fig. 4 Ginkgo total lactones assay HPLC collection of illustrative plates (example 1 sample)
The specific embodiment
Embodiment 1:
Claiming 7.2g pharmagel (osseocolla) slowly to add under stirring is heated in about 40 ℃ 4800ml purified water in advance.Insulation, stirring all are dissolved into uniform solution until pharmagel and are promptly got 0.15% pharmagel aqueous solution (g/v), and be standby.
Claim that (Folium Ginkgo extract is that our company produces to the 80g Folium Ginkgo extract, product batch number 0691013, total flavonoids content is 24.4%, terpene lactone contents is 8.5%, moisture 3.4%, flavone aglycone peak area ratio 0.9) in the above-mentioned concentration pharmagel of the impouring aqueous solution, heat to 100 ℃, insulated and stirred was extracted 30 minutes.Extract to finish, extracting solution cools to 10 ℃, leaves standstill, sedimentation 24 hours, and solution filters, and solids discards, filtrate for later use.
Above-mentioned filtrate is 10000 ultrafilter membrane ultrafiltration with molecular cut off, collects ultrafiltrate (molecular weight＜10000).Ultrafiltrate 80 ℃ ,-carry out concentrating under reduced pressure under the condition of 0.085Mpa, be concentrated to the raffinate scale of construction and be 1/24 o'clock of former ultrafiltrate volume, stop to concentrate.Concentrated solution cold preservation crystallize 36 hours under 5 ℃ of conditions filters, and keeps filtrate, filter cake with 100ml cold water soak into, washing keeps washing liquid 3 times, drain; Filter cake places the vacuum drying oven drying under reduced pressure, baking temperature: 80 ℃, vacuum :-0.1MPa, obtains off-white color ginkgo terpene total lactones 6.2g at 4 hours drying times.According to " the ginkgo terpene total lactones detection method detects in 2005 editions one relevant Folium Ginkgo extract prescription of Chinese pharmacopoeia, and ginkgo terpene total lactones content is: 57.8%.
The water washing liquor that keeps during the preparation ginkgo terpene total lactones and the filtrate of reservation merge, 80 ℃ ,-condition of 0.085Mpa under concentrating under reduced pressure become fluid extract (relative density: 1.250), in fluid extract, add 95% ethanol 2400ml, heating, 60 ℃ of Wen Rong fully are dissolved in 95% alcoholic solution active substance, to wherein adding the 6.0g active carbon, insulation, stirring 30 minutes, solution cold preservation under 5 ℃ of conditions is left standstill, sedimentation 24 hours, filters, solids discards filtrate for later use.
With above-mentioned filtrate 80 ℃ ,-carry out concentrating under reduced pressure under the condition of 0.085Mpa and become fluid extract (relative density: 1.250), fluid extract is placed the vacuum drying oven drying under reduced pressure, baking temperature: 80 ℃, vacuum :-0.1MPa, 4 hours drying times, dry thing carries out the pulverizing of 80 orders and obtains faint yellow Ginkgo total flavones extract 60.8g.According to " 2005 editions relevant Folium Ginkgo extract prescriptions of Chinese pharmacopoeia: measure Ginkgo total flavones alcohol glycosides and ginkgo terpene total lactones content in the product.Ginkgo total flavones alcohol glycosides content is: 31.23%; Ginkgo terpene total lactones does not detect.
According to the content that detects back two kinds of compositions, by Ginkgo total flavones alcohol glycosides content: it is always mixed that ginkgo terpene total lactones content is that 4: 1 content ratio is carried out, and promptly obtains a kind of water-soluble ginkgo biloba extract used for injection.
Embodiment 2:
Claiming 14.4g pharmagel (osseocolla) slowly to add under stirring is heated in about 40 ℃ 4800ml purified water in advance.Insulation, stirring all are dissolved into uniform solution until pharmagel and are promptly got 0.30% pharmagel aqueous solution (g/v), and be standby.
Claim that (Folium Ginkgo extract is that our company produces to the 80g Folium Ginkgo extract, product batch number 0691013, total flavonoids content is 24.4%, terpene lactone contents is 8.5%, moisture 3.4%, flavone aglycone peak area ratio 0.9) in the above-mentioned concentration pharmagel of the impouring aqueous solution, heat to 100 ℃, insulated and stirred was extracted 30 minutes.Extract to finish, extracting solution cools to 10 ℃, leaves standstill, sedimentation 24 hours, and solution filters, and solids discards, filtrate for later use.
Above-mentioned filtrate is 10000 ultrafilter membrane ultrafiltration with molecular cut off, collects ultrafiltrate (molecular weight＜10000).Ultrafiltrate 80 ℃ ,-carry out concentrating under reduced pressure under the condition of 0.085Mpa, be concentrated to the raffinate scale of construction and be 1/24 o'clock of former ultrafiltrate volume, stop to concentrate.Concentrated solution cold preservation crystallize 36 hours under 5 ℃ of conditions filters, and keeps filtrate, filter cake with 100ml cold water soak into, washing keeps washing liquid 3 times, drain; Filter cake places the vacuum drying oven drying under reduced pressure, baking temperature: 80 ℃, vacuum :-0.1MPa, obtains off-white color ginkgo terpene total lactones 5.8g at 4 hours drying times.According to " the ginkgo terpene total lactones detection method detects in 2005 editions one relevant Folium Ginkgo extract prescription of Chinese pharmacopoeia, and ginkgo terpene total lactones content is: 62.3%.
The water washing liquor that keeps during the preparation ginkgo terpene total lactones and the filtrate of reservation merge, 80 ℃ ,-condition of 0.085Mpa under concentrating under reduced pressure become fluid extract (relative density: 1.250), in fluid extract, add 95% ethanol 2400ml, heating, 60 ℃ of Wen Rong fully are dissolved in 95% alcoholic solution active substance, to wherein adding the 6.0g active carbon, insulation, stirring 30 minutes, solution cold preservation under 5 ℃ of conditions is left standstill, sedimentation 24 hours, filters, solids discards filtrate for later use.
With above-mentioned filtrate 80 ℃ ,-carry out concentrating under reduced pressure under the condition of 0.085Mpa and become fluid extract (relative density: 1.250), fluid extract is placed the vacuum drying oven drying under reduced pressure, baking temperature: 80 ℃, vacuum :-0.1MPa, 4 hours drying times, dry thing carries out the pulverizing of 80 orders and obtains faint yellow Ginkgo total flavones extract 58.6g.According to " 2005 editions relevant Folium Ginkgo extract prescriptions of Chinese pharmacopoeia: measure Ginkgo total flavones alcohol glycosides and ginkgo terpene total lactones content in the product.Ginkgo total flavones alcohol glycosides content is: 33.7%; Ginkgo terpene total lactones does not detect.
According to the content that detects back two kinds of compositions, by Ginkgo total flavones alcohol glycosides content: it is always mixed that ginkgo terpene total lactones content is that 4: 1 content ratio is carried out, and promptly obtains a kind of water-soluble ginkgo biloba extract used for injection.
Embodiment 3:
Claiming 4.8g pharmagel (osseocolla) slowly to add under stirring is heated in about 40 ℃ 4800ml purified water in advance.Insulation, stirring all are dissolved into uniform solution until pharmagel and are promptly got 0.10% pharmagel aqueous solution (g/v), and be standby.
Claim that (Folium Ginkgo extract is that our company produces to the 80g Folium Ginkgo extract, product batch number 0691013, total flavonoids content is 24.4%, terpene lactone contents is 8.5%, moisture 3.4%, flavone aglycone peak area ratio 0.9) in the above-mentioned concentration pharmagel of the impouring aqueous solution, heat to 100 ℃, insulated and stirred was extracted 30 minutes.Extract to finish, extracting solution cools to 10 ℃, leaves standstill, sedimentation 24 hours, and solution filters, and solids discards, filtrate for later use.
Above-mentioned filtrate is 10000 ultrafilter membrane ultrafiltration with molecular cut off, collects ultrafiltrate (molecular weight＜10000).Ultrafiltrate 80 ℃ ,-carry out concentrating under reduced pressure under the condition of 0.085Mpa, be concentrated to the raffinate scale of construction and be 1/24 o'clock of former ultrafiltrate volume, stop to concentrate.Concentrated solution cold preservation crystallize 36 hours under 5 ℃ of conditions filters, and keeps filtrate, filter cake with 100ml cold water soak into, washing keeps washing liquid 3 times, drain; Filter cake places the vacuum drying oven drying under reduced pressure, baking temperature: 80 ℃, vacuum :-0.1MPa, obtains off-white color ginkgo terpene total lactones 6.4g at 4 hours drying times.According to " the ginkgo terpene total lactones detection method detects in 2005 editions one relevant Folium Ginkgo extract prescription of Chinese pharmacopoeia, and ginkgo terpene total lactones content is: 58.6%.
The water washing liquor that keeps during the preparation ginkgo terpene total lactones and the filtrate of reservation merge, 80 ℃ ,-condition of 0.085Mpa under concentrating under reduced pressure become fluid extract (relative density: 1.250), in fluid extract, add 95% ethanol 2400ml, heating, 60 ℃ of Wen Rong fully are dissolved in 95% alcoholic solution active substance, to wherein adding the 6.0g active carbon, insulation, stirring 30 minutes, solution cold preservation under 5 ℃ of conditions is left standstill, sedimentation 24 hours, filters, solids discards filtrate for later use.
With above-mentioned filtrate 80 ℃ ,-carry out concentrating under reduced pressure under the condition of 0.085Mpa and become fluid extract (relative density: 1.250), fluid extract is placed the vacuum drying oven drying under reduced pressure, baking temperature: 80 ℃, vacuum :-0.1MPa, 4 hours drying times, dry thing carries out the pulverizing of 80 orders and obtains faint yellow Ginkgo total flavones extract 62.1g.According to " 2005 editions relevant Folium Ginkgo extract prescriptions of Chinese pharmacopoeia: measure Ginkgo total flavones alcohol glycosides and ginkgo terpene total lactones content in the product.Ginkgo total flavones alcohol glycosides content is: 30.8%; Ginkgo terpene total lactones does not detect.
According to the content that detects back two kinds of compositions, by Ginkgo total flavones alcohol glycosides content: it is always mixed that ginkgo terpene total lactones content is that 4: 1 content ratio is carried out, and promptly obtains a kind of water-soluble ginkgo biloba extract used for injection.
Claims (4)
1. the preparation technology of an injection water soluble ginkgo leaf extract, it is characterized in that: this technology comprises the following steps:
(1) preparation 0.1%～0.3% pharmagel aqueous solution g/v: in pharmagel: purified water is 0.1～0.3: 100 ratio w/v, pharmagel slowly adds under stirring and is heated in advance in about 40 ℃ purified water, insulation, stirring all are dissolved into uniform solution until gelatin and are promptly got 0.1%～0.3% pharmagel aqueous solution g/v, and be standby;
(2) dissolving Folium Ginkgo extract: Folium Ginkgo extract adds in 0.1%～0.3% pharmagel aqueous solution, heats to 80 ℃～100 ℃, and insulated and stirred was extracted 30 minutes～60 minutes, the solution natural cooling, leave standstill, sedimentation 24 hours～36 hours, solution filters, insoluble matter discards;
(3) ultrafiltration: filtrate is used the ultrafilter membrane ultrafiltration, collect ultrafiltrate, ultrafiltrate 60 ℃～80 ℃ ,-0.085～-carry out concentrating under reduced pressure under the condition of 0.1Mpa, be concentrated to the raffinate scale of construction and be 1/16 of former ultrafiltrate volume～1/24 o'clock, stop to concentrate, concentrated solution cold preservation crystallize 24 hours～36 hours under 5 ℃～10 ℃ conditions, filter, keep filtrate, filter cake cold water washing 2～4 times keep washing liquid;
(4) vacuum drying prepares ginkgo terpene total lactones: step (3) filter cake places the vacuum drying oven drying under reduced pressure, baking temperature: 60 ℃～80 ℃, vacuum :-0.1MPa, obtains the off-white color ginkgo terpene total lactones at 3～4 hours drying times;
(5) preparation Ginkgo total flavones extract
A: concentrating under reduced pressure, decolouring: the cleaning mixture of step (3) and filtrate merge, at 60 ℃～80 ℃,-0.085～-condition of 0.1Mpa under concentrating under reduced pressure become the fluid extract relative density: 1.200～1.300,95% ethanol that in fluid extract, adds 30～50 times of amounts of Folium Ginkgo extract inventory, heating, 60 ℃～80 ℃ Wen Rong, active substance fully is dissolved in 95% alcoholic solution, to the active carbon that wherein adds 0.25%～0.5%W/V, insulation, stirred 30 minutes, solution cold preservation under 5 ℃～10 ℃ conditions is left standstill, sedimentation 24 hours～36 hours, filter, solids discards, and filtrate is stand-by;
B: with above-mentioned filtrate 60 ℃～80 ℃ ,-0.085～-carry out concentrating under reduced pressure under the condition of 0.1Mpa and become the fluid extract relative density: 1.200～1.300, fluid extract is placed the vacuum drying oven drying under reduced pressure, baking temperature: 60 ℃～80 ℃, vacuum :-0.1MPa, 3～4 hours drying times, dry thing carries out the pulverizing of 80 orders and obtains faint yellow Ginkgo total flavones extract;
(6) preparation finished product: the Ginkgo total flavones extract that the ginkgo terpene total lactones that above-mentioned steps (4) obtains and step (5) obtain is according to " method of 2005 editions one relevant Folium Ginkgo extract prescription regulation of Chinese pharmacopoeia detects the content of ginkgo terpene total lactones and Ginkgo total flavones alcohol glycosides respectively, by Ginkgo total flavones alcohol glycosides content: ginkgo terpene total lactones content is that 4: 1 content ratio is carried out total mixing, promptly obtains the water soluble ginkgo leaf extract that ejection preparation is used.
2. the preparation technology of injection water soluble ginkgo leaf extract according to claim 1 is characterized in that: the consumption of the pharmagel aqueous solution of step (2) described 0.1%～0.3% is 40 times～100 times W/V of Folium Ginkgo extract weight.
3. the preparation technology of injection water soluble ginkgo leaf extract according to claim 1 is characterized in that: aqueous solution filtrate is that 3000～10000 ultrafilter membrane carries out ultrafiltration with molecular cut off.
4. the preparation technology of injection water soluble ginkgo leaf extract according to claim 1, Ginkgo total flavones alcohol glycosides content in the Semen Ginkgo extrac that it is characterized in that making: ginkgo terpene total lactones content is 4: 1.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100385066A CN101596221B (en) | 2008-06-04 | 2008-06-04 | Process for preparing water-soluble ginkgo biloba extract used for injection |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008100385066A CN101596221B (en) | 2008-06-04 | 2008-06-04 | Process for preparing water-soluble ginkgo biloba extract used for injection |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101596221A true CN101596221A (en) | 2009-12-09 |
| CN101596221B CN101596221B (en) | 2012-06-20 |
Family
ID=41417938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008100385066A Active CN101596221B (en) | 2008-06-04 | 2008-06-04 | Process for preparing water-soluble ginkgo biloba extract used for injection |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101596221B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108383852A (en) * | 2017-12-25 | 2018-08-10 | 上海信谊百路达药业有限公司 | A kind of Ginkgolid extracted from ginkgo leaf and its preparation |
| CN114848690A (en) * | 2022-05-31 | 2022-08-05 | 江苏得乐康生物科技有限公司 | Preparation method of low biflavone water-soluble ginkgo leaf extract suitable for liquid preparation |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094385C (en) * | 1999-01-25 | 2002-11-20 | 南开大学 | Modified natural macromolecular compound adsorbent and application thereof |
| CN1430968A (en) * | 2003-01-30 | 2003-07-23 | 复旦大学 | Injection liquid of extractive gingko leaves and its preparing method |
| CN1772009A (en) * | 2005-10-19 | 2006-05-17 | 何照范 | Production process of water soluble ginkgo leaf extract |
-
2008
- 2008-06-04 CN CN2008100385066A patent/CN101596221B/en active Active
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108383852A (en) * | 2017-12-25 | 2018-08-10 | 上海信谊百路达药业有限公司 | A kind of Ginkgolid extracted from ginkgo leaf and its preparation |
| CN108383852B (en) * | 2017-12-25 | 2019-11-22 | 上海信谊百路达药业有限公司 | A kind of Ginkgolid extracted from ginkgo leaf and its preparation |
| CN114848690A (en) * | 2022-05-31 | 2022-08-05 | 江苏得乐康生物科技有限公司 | Preparation method of low biflavone water-soluble ginkgo leaf extract suitable for liquid preparation |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101596221B (en) | 2012-06-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100503626C (en) | Producing raw material containing benzyl carbinol glycosides from Cistanche deserticola by using membrane separation technique and preparation method thereof | |
| CN104840501B (en) | A kind of preparation method of chrysanthemum total flavone | |
| CN106943768A (en) | Tea Polyphenols and its extracting method and application | |
| CN101254220A (en) | Production process of water soluble ginkgo leaf extractive | |
| CN101596221B (en) | Process for preparing water-soluble ginkgo biloba extract used for injection | |
| CN112957379B (en) | Method for extracting centella asiatica extract | |
| CN110496179A (en) | A kind of method and application for rapidly and efficiently extracting antioxidant in Areca | |
| CN107674066A (en) | A kind of method that Isoschaftoside is extracted from Desmodium styracifolium | |
| CN101278972B (en) | Technique for preparing Salvia miltiorrhiza injection with pH gradient sedimentation method combined with hyperfiltration technique | |
| CN103655655A (en) | Method for separating active ingredients from medicinal material by membrane technology | |
| CN101683363B (en) | Composition preparation of ginkgo biloba extract and dipyridamole and preparation method thereof | |
| CN101297884B (en) | Prescription of low-dose condensed type Mai jun an tablet or capsule and preparation thereof | |
| CN108707142A (en) | A kind of Retreatment method of pueraria starch processing slag and effluent | |
| CN100482196C (en) | An extraction preparation method of effective ingredients of Chinese medicine | |
| CN107652258A (en) | Quercetin, the method for isoquercitrin are extracted from beggar-ticks | |
| CN107556277A (en) | Quercetin, the method for isoquercitrin are extracted from hyperici,herba | |
| CN107840844A (en) | A kind of method that Schaftoside is extracted from Desmodium styracifolium | |
| CN101780122B (en) | Preparation method of a water-soluble gingko extract | |
| CN105395583A (en) | Preparing method for acanthopanax senticosus extract | |
| CN102070686A (en) | Method for extracting rutin from pagodatree flower buds by microwave method | |
| CN108938709B (en) | Water extract of tobacco bean and application thereof | |
| CN107840862A (en) | A kind of method that isoquercitrin is extracted from Desmodium styracifolium | |
| CN107652259A (en) | A kind of method that Quercetin is extracted from Desmodium styracifolium | |
| CN107840863A (en) | The method that isoquercitrin is extracted from hyperici,herba | |
| CN107652179A (en) | A kind of method that forulic acid is extracted from Desmodium styracifolium |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |