CN101589854A - A kind of air filter type bacterial cellulose mask and production method thereof - Google Patents
A kind of air filter type bacterial cellulose mask and production method thereof Download PDFInfo
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- CN101589854A CN101589854A CNA2009101496658A CN200910149665A CN101589854A CN 101589854 A CN101589854 A CN 101589854A CN A2009101496658 A CNA2009101496658 A CN A2009101496658A CN 200910149665 A CN200910149665 A CN 200910149665A CN 101589854 A CN101589854 A CN 101589854A
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- bacteria cellulose
- nano silver
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- mask
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- 229920002749 Bacterial cellulose Polymers 0.000 title claims abstract description 27
- 239000005016 bacterial cellulose Substances 0.000 title claims abstract description 27
- 238000004519 manufacturing process Methods 0.000 title claims abstract description 12
- 241000894006 Bacteria Species 0.000 claims abstract description 72
- 229920002678 cellulose Polymers 0.000 claims abstract description 69
- 239000001913 cellulose Substances 0.000 claims abstract description 69
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 claims abstract description 39
- 239000000463 material Substances 0.000 claims abstract description 32
- 235000002837 Acetobacter xylinum Nutrition 0.000 claims abstract description 17
- 238000002360 preparation method Methods 0.000 claims abstract description 14
- 238000001035 drying Methods 0.000 claims abstract description 11
- 150000001875 compounds Chemical class 0.000 claims abstract description 6
- 239000002253 acid Substances 0.000 claims abstract description 3
- 238000007598 dipping method Methods 0.000 claims abstract description 3
- 241001136169 Komagataeibacter xylinus Species 0.000 claims abstract 4
- 230000001580 bacterial effect Effects 0.000 claims description 22
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 16
- 239000001301 oxygen Substances 0.000 claims description 16
- 229910052760 oxygen Inorganic materials 0.000 claims description 16
- 238000000855 fermentation Methods 0.000 claims description 14
- 230000004151 fermentation Effects 0.000 claims description 14
- 238000004108 freeze drying Methods 0.000 claims description 14
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(1+) nitrate Chemical compound [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 14
- 239000012530 fluid Substances 0.000 claims description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 9
- 239000008367 deionised water Substances 0.000 claims description 8
- 229940071575 silver citrate Drugs 0.000 claims description 7
- 229910001961 silver nitrate Inorganic materials 0.000 claims description 7
- QUTYHQJYVDNJJA-UHFFFAOYSA-K trisilver;2-hydroxypropane-1,2,3-tricarboxylate Chemical compound [Ag+].[Ag+].[Ag+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O QUTYHQJYVDNJJA-UHFFFAOYSA-K 0.000 claims description 7
- 238000000746 purification Methods 0.000 claims description 6
- SDLBJIZEEMKQKY-UHFFFAOYSA-M silver chlorate Chemical compound [Ag+].[O-]Cl(=O)=O SDLBJIZEEMKQKY-UHFFFAOYSA-M 0.000 claims description 6
- RGHNJXZEOKUKBD-SQOUGZDYSA-M D-gluconate Chemical group OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C([O-])=O RGHNJXZEOKUKBD-SQOUGZDYSA-M 0.000 claims description 5
- 230000004913 activation Effects 0.000 claims description 5
- 229940050410 gluconate Drugs 0.000 claims description 5
- XNGYKPINNDWGGF-UHFFFAOYSA-L silver oxalate Chemical compound [Ag+].[Ag+].[O-]C(=O)C([O-])=O XNGYKPINNDWGGF-UHFFFAOYSA-L 0.000 claims description 4
- 230000003248 secreting effect Effects 0.000 claims description 2
- 230000001954 sterilising effect Effects 0.000 abstract description 17
- 238000004659 sterilization and disinfection Methods 0.000 abstract description 16
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 7
- 238000001179 sorption measurement Methods 0.000 abstract description 5
- 238000005516 engineering process Methods 0.000 abstract description 4
- 238000001914 filtration Methods 0.000 abstract description 3
- 239000002574 poison Substances 0.000 abstract description 3
- 231100000614 poison Toxicity 0.000 abstract description 3
- 238000002791 soaking Methods 0.000 abstract description 3
- 230000002045 lasting effect Effects 0.000 abstract description 2
- 244000005700 microbiome Species 0.000 description 16
- 244000235858 Acetobacter xylinum Species 0.000 description 13
- HEMHJVSKTPXQMS-UHFFFAOYSA-M sodium hydroxide Inorganic materials [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 11
- 230000000694 effects Effects 0.000 description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 9
- 239000002245 particle Substances 0.000 description 8
- 241000588724 Escherichia coli Species 0.000 description 7
- 241000186779 Listeria monocytogenes Species 0.000 description 7
- 241000191967 Staphylococcus aureus Species 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 244000000010 microbial pathogen Species 0.000 description 7
- IAYPIBMASNFSPL-UHFFFAOYSA-N Ethylene oxide Chemical compound C1CO1 IAYPIBMASNFSPL-UHFFFAOYSA-N 0.000 description 6
- 235000020415 coconut juice Nutrition 0.000 description 6
- 229910021641 deionized water Inorganic materials 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 5
- 241000222122 Candida albicans Species 0.000 description 4
- 241000607142 Salmonella Species 0.000 description 4
- 229940095731 candida albicans Drugs 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 239000002609 medium Substances 0.000 description 4
- 239000002994 raw material Substances 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- OTMSDBZUPAUEDD-UHFFFAOYSA-N Ethane Chemical compound CC OTMSDBZUPAUEDD-UHFFFAOYSA-N 0.000 description 3
- 239000004599 antimicrobial Substances 0.000 description 3
- 230000015572 biosynthetic process Effects 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000004060 metabolic process Effects 0.000 description 3
- 238000005554 pickling Methods 0.000 description 3
- 230000002265 prevention Effects 0.000 description 3
- 239000011347 resin Substances 0.000 description 3
- 229920005989 resin Polymers 0.000 description 3
- 230000000241 respiratory effect Effects 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- 238000012935 Averaging Methods 0.000 description 1
- 206010019345 Heat stroke Diseases 0.000 description 1
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 239000000370 acceptor Substances 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 238000004220 aggregation Methods 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004891 communication Methods 0.000 description 1
- 230000001276 controlling effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000003795 desorption Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000027721 electron transport chain Effects 0.000 description 1
- 230000001815 facial effect Effects 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 206010022000 influenza Diseases 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 238000010297 mechanical methods and process Methods 0.000 description 1
- 230000005226 mechanical processes and functions Effects 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000000505 pernicious effect Effects 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 210000002345 respiratory system Anatomy 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- -1 silver ions Chemical class 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000035900 sweating Effects 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
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- Filtering Materials (AREA)
- Apparatus For Disinfection Or Sterilisation (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of air filter type bacterial cellulose mask and production method thereof, wherein mouth mask be with through the bacteria cellulose of super-dry as the mouth mask frame material through being made; Its preparation method is to choose acetobacter xylinum as type strain, makes the bacteria cellulose diaphragm through cultivating; Diaphragm is washed behind acid solution, dipping by lye to percentage of admixture＜0.5%, be infiltrated on again in the Nano silver solution, adopt cryodesiccated mode to carry out drying then, cross gas filtering type bacterial cellulose mask through the compound air for preparing again.Preparation technology of the present invention is simple; with the bacteria cellulose is that material can improve the mouth mask strainability effectively; by soaking into Nano Silver the sterilization poison resistance is strengthened; have characteristics such as production cost is low, high adsorption capacity, bactericidal activity height, lasting long sterilizing time; can reduce resource consumption effectively, preserve the ecological environment.
Description
Technical field
The present invention relates to a kind of air filter type bacterial cellulose mask and production method thereof, belong to technical field of bioengineering.
Background technology
The mouth mask Prosthesis that to be the material that a kind ofly can be covered in human body face, stop specific standard and attribute directly contact with human respiratory.
As everyone knows, in the multiple disease that the mankind suffer from air as communication media, the example that spreads, infects as the route of transmission with respiratory apparatus such as mouth and nose can be found everywhere, its consequence that causes makes us so tragic that one cannot bear to look at it, and only the big influenza of Spain's outburst in 1918 has just caused nearly 5,000 ten thousand people's death.We know that this is caused by the outburst of the madness of microorganism now.Based on this, people recognize wear mouth mask effectively prevention and control some via airborne disease.
But because the difference of the kind of microorganism, the prevention and control different microorganisms just has different requirements to the material and the structure of mouth mask.The mouth mask that widely people utilized at present is the air filter type mouth mask, and its operation principle is to be sucked by human body after making the filter course purification filtering of air by mouth mask that contains harmful substance again.It is huge to it is found that present widely used mouth mask is limited to the difference on effect of its prevention and control disease of the aperture of material, adsorption capacity and sterilizing ability, and in the process of using if hot gas or the saliva breathed out are got wet, its effect that intercepts germ will reduce greatly.
Attempt to adopt the methods such as interdiction capability that increase the compound number of plies, strengthen filter course to improve in order to improve filter efficiency, people, though these methods have improved barriering effect to a certain extent, its side effect is more obvious.
1, the increase of the filtering layer number net weight that not only strengthened mouth mask also can cause mouth mask air mass transfer ability drop, and it is too high to cause facial local temperature easily, occurs sweating easilier, pernicious situation such as unsmooth breath, anoxic, heatstroke.
2, the process of filter course absorption impurity is a reversible absorption a--desorption process, after adsorbance acquires a certain degree, though its adsorption capacity does not have significant change, but its analytic ability can increase greatly simultaneously, so the impurity that desorb this moment is got off causes secondary pollution with regard to probably enter the respiratory system of human body by filtrate.
3, can continuous enrichment get up attached to the various microorganisms on the sorbing material, still there is not obvious influence at the mouth mask initial stage of using, but along with the microorganism total amount of enrichment is more and more, when reaching a certain critical value, mouth mask will become breed " base " of microorganism, makes it become the new infection sources.
Though have the people to propose to adopt modes such as loading the medicine sheath to attempt at present, all be difficult to the marketization because of cost is too high to the improvement of mouth mask.
Summary of the invention
The objective of the invention is provides a kind of air filter type bacterial cellulose mask at the deficiencies in the prior art; with the bacteria cellulose is that material can improve the mouth mask strainability effectively; by soaking into Nano Silver the sterilization poison resistance is strengthened; have characteristics such as production cost is low, high adsorption capacity; can reduce resource consumption effectively, preserve the ecological environment.
Another object of the present invention provides a kind of preparation method of above-mentioned air filter type bacterial cellulose mask.
By selecting suitable type strain for use, suitable culture condition and processing method being provided, produce suitable bacteria cellulose material, again bacteria cellulose material is combined the final air filter type bacterial cellulose mask that forms after plastic working with the sterilization counterpoison.
The technology used in the present invention principle:
1, utilize acetobacter xylinum can metabolism under the condition that has oxygen to exist to produce the characteristic of bacteria cellulose, metabolism produces the bacteria cellulose material that quality is even, moral character is good on the gas-liquid two-phase interface of culture medium.
Thickness, porosity, compactness extent and the architectural feature of new green material all can be controlled by adjusting the controllable parameters such as unit intensity, unit pressure and release time that discharge oxygen.
2, utilize silver ion particularly Nano Silver have the characteristic of strong effect wide-spectrum antibacterial ability, ionic state Nano Silver base-material homogeneous is soaked into bacteria cellulose material, be the mouth mask filter course more after drying and incorporate effective antimicrobial component.In respiratory, steam soaks into behind the mouth mask can dissolving part nano-Ag particles, discharges enough antimicrobial silver ions, therefore can effectively kill the microorganisms such as bacterium, virus of enrichment, protects from infection.
Because nano-Ag particles character is very active, easily by high-temperature oxydation, be difficult to keep the bactericidal activity of Nano Silver,, it is combined closely with bacteria cellulose material under the state of stable homogeneous and keep its high activity so adopt cryodesiccated mode that nano-Ag particles is fixed.
The sterilizing mechanisms of Nano Silver is as follows:
1, the oxygen in can ionized air produces ionic state oxygen, and the strong oxidability of ionic state oxygen is killing microorganisms effectively.
2, Nano Silver reactivity height is easy to combined with protein under normal conditions, has very strong adsorpting aggregation ability, and synthetic process is destroyed the purpose that its normal physiology course reaches sterilization thereby the while Nano Silver can participate in the metabolism of microorganism.
3, the Nano Silver electron transport chain structural system that can enter into microorganism is gone, and electron acceptors such as emulative replacement oxygen interrupt the energetic supersession process of microorganism, impel thalline dead fast.
The technical scheme that the present invention is adopted for its technical problem of solution is as follows:
A kind of air filter type bacterial cellulose mask, be with through the bacteria cellulose of super-dry as the mouth mask frame material through being made.
Above-mentioned air filter type bacterial cellulose mask is sewed up and is made by carrying out flanging after compound through cryodesiccated three layers of bacteria cellulose diaphragm, and wherein two skins are the bacteria cellulose filter course, and the intermediate layer is the bacteria cellulose stenlizing layer; Described bacteria cellulose stenlizing layer is to be infiltrated on nano silver-group solution after freeze drying and making by the bacteria cellulose diaphragm; Described nano silver-group solution is meant and the Nano Silver base-material is dissolved in the water to form concentration be the solution of 0.001～0.050mol/L; Described Nano Silver base-material is meant silver nitrate, silver chlorate, silver citrate or silver oxalate.
The thickness of described bacteria cellulose layer is 0.1～3.0mm.
A kind of production method of above-mentioned air filter type bacterial cellulose mask, its concrete steps are as follows:
1, apolegamy bacterial classification
Choose the cellulosic bacterial classification of energy secreting bacteria as type strain, the type strain activation is prepared into the seed mash, throw in the seed mash then and to fermentation medium, make the initial zymotic fluid that contains active bacterial strain.
Described bacterial classification is that acetobacter xylinum belongs to bacterial strain.
It is gluconate pyracetobacillus 323 that described acetobacter xylinum belongs to bacterial strain.
The concentration of bacterial strain is 0.5%～95% in the described seed mash.
2, logical aerobe fermentation
The initial zymotic fluid that will contain active bacterial strain is inserted in the culture tank, feeds oxygen-enriched air in liquid surface, makes the acetobacter xylinum that is positioned at the gas-liquid two-phase interface breed the justacrine bacteria cellulose in a large number, obtains the bacteria cellulose diaphragm.Can control the parameters such as porosity, thickness of bacteria cellulose material by concentration, fermentation time, the oxygen partial pressure of regulating type strain, form the bacteria cellulose diaphragm of different size.
3, purification process
The bacteria cellulose diaphragm that makes is spent deionised water respectively to percentage of admixture＜0.5% behind acid solution, dipping by lye, after packaged sterile, be stored in again in 2～4 ℃ of refrigerators preserve standby.
4, preparation stenlizing layer
The Nano Silver base-material is dissolved in the water, and to form Nano Silver concentration be the nano silver-group solution of 0.001～0.050mol/L, to be infiltrated in the nano silver-group solution behind the Kaifeng of the bacteria cellulose diaphragm after the packaged sterile again, when Nano Silver concentration reaches 0.001～0.050mol/L in the diaphragm, stop to soak into, diaphragm is obtained the bacteria cellulose stenlizing layer through freeze drying.Described Nano Silver base-material is meant silver nitrate, silver chlorate, silver citrate or silver oxalate.
5, drying
Carry out preserving in the rearmounted sterile bag of freeze drying the bacteria cellulose diaphragm that does not soak into nano silver-group solution stand-by.
6, mouth mask preparation
Will be compound through cryodesiccated two layers of bacteria cellulose diaphragm and one deck bacteria cellulose stenlizing layer be three layers, wherein two skins are the bacteria cellulose diaphragm, the intermediate layer is the bacteria cellulose stenlizing layer, carry out flanging then and be stitched into one, make the mouth mask rope, make air filter type bacterial cellulose mask.
The silver nitrate substrate nano silver particle diameter that the present invention adopts is about 20 nanometers, and the bacterial cellulose mask that is fixed with nano-Ag particles after tested has good disinfection effect to multiple microorganism, and each sterilizing value is got the multiple averaging value, sees following table for details:
| Pathogenic microorganisms | Escherichia coli | Staphylococcus aureus | Listeria monocytogenes | Candida albicans | Salmonella |
| The microorganism fatal rate | ＞97％ | ＞98％ | ＞96％ | ＞96％ | ＞98％ |
Above-mentioned every sterilization index is all far above national standard.
Preparation technology of the present invention is simple; with the bacteria cellulose is that material can improve the mouth mask strainability effectively; by soaking into Nano Silver the sterilization poison resistance is strengthened; have characteristics such as production cost is low, high adsorption capacity, bactericidal activity height, lasting long sterilizing time; can reduce resource consumption effectively, preserve the ecological environment.
The specific embodiment
Below in conjunction with non-limiting embodiment the present invention is described in further detail.
Embodiment one
1) apolegamy bacterial classification
Choose gluconate pyracetobacillus 323 as type strain, it is 5% acetobacter xylinum seed mash that type strain activation is prepared into concentration, acetobacter xylinum seed mash is seeded to makes the initial zymotic fluid that contains the acetobacter xylinum active bacterial strain in the coconut water by fermentation culture medium.
2) logical aerobe fermentation
It is in the culture tank of 30mm that initial zymotic fluid is inserted coconut water by fermentation liquid medium layer height, inserts 29 ℃ constant incubator together with culture vessel and cultivates 2d.Open the oxygen supply valve simultaneously, continuing to feed pressure to the culture tank top is the medical oxygen of 0.075MP, and results thickness is about the bacteria cellulose diaphragm of 0.2mm.
3) purification process
Above-mentioned bacteria cellulose diaphragm is soaked 1h, 0.1mol chlorohydric acid pickling 0.5h with the 0.5mol hydrochloric acid solution; Soak 1h with the 0.5mol sodium hydroxide solution again; Then soak 1h, wash repeatedly to percentage of admixture＜0.5% with deionized water then with deionized water, be stored in after the ethane via epoxyethane packaged sterile again preserve in 2 ℃ of refrigerators standby.
4) preparation stenlizing layer
Select for use silver nitrate as the Nano Silver base-material, with its dissolving and formation Nano Silver concentration is the nano silver-group solution of 0.02mol/L, to be infiltrated in the nano silver-group solution behind the Kaifeng of the bacteria cellulose diaphragm after the packaged sterile again, when Nano Silver concentration reaches 0.02mol/L in the material, stop to soak into, diaphragm is obtained the bacteria cellulose stenlizing layer through freeze drying.
5) drying
With the bacteria cellulose diaphragm that does not soak into nano silver-group solution place preserve in the rearmounted sterile bags of-80 ℃ of freeze dryer freeze dryings stand-by.
6) mouth mask preparation
The bacteria cellulose diaphragm that does not soak into nano silver-group solution with drying is that raw material is made the film bag, the bacteria cellulose stenlizing layer that carries nano silver antimicrobials of drying is packed in the film bag, carry out flanging again and be stitched into one, make the mouth mask rope, make air filter type bacterial cellulose mask.
7) sterilization
The air filter type bacterial cellulose mask that makes is put 800 ℃, and 20 atmospheric pressure are handled 1h down, the oxirane sterilization, and the rearmounted 4 ℃ of refrigerators of medical resin container vacuum sealed package are preserved standby.
8) bacteriostasis property test
Choose typical pathogenic microorganisms such as Escherichia coli, staphylococcus aureus, Listeria monocytogenes and do the bactericidal property test
The silver nitrate substrate nano silver particle diameter that the present invention adopts is about 20 nanometers, and disinfection effect sees the following form:
| Pathogenic microorganisms | Escherichia coli | Staphylococcus aureus | Listeria monocytogenes | Candida albicans | Salmonella |
| The microorganism fatal rate | 97.56％ | 98.20％ | 96.88％ | 96.59％ | 98.97％ |
Embodiment two
1) apolegamy bacterial classification
Choose gluconate pyracetobacillus 323 as type strain, it is 5% acetobacter xylinum seed mash that type strain activation is prepared into concentration, acetobacter xylinum seed mash is seeded to makes the initial zymotic fluid that contains the acetobacter xylinum active bacterial strain in the coconut water by fermentation culture medium.
2) logical aerobe fermentation
It is in the culture tank of 30mm that initial zymotic fluid is inserted coconut water by fermentation liquid medium layer height, inserts 30 ℃ constant incubator together with culture vessel and cultivates 2d.Open the oxygen supply valve simultaneously, continuing to feed pressure to the culture tank top is the medical oxygen of 0.075MP, and results thickness is about the bacteria cellulose diaphragm of 0.5mm.
3) purification process
Above-mentioned bacteria cellulose diaphragm is soaked 1h, 0.1mol chlorohydric acid pickling 0.5h with the 0.5mol hydrochloric acid solution; Soak 1h with the 0.5mol sodium hydroxide solution again; Then soak 1h, wash repeatedly to percentage of admixture＜0.5% with deionized water then with deionized water, be stored in after the ethane via epoxyethane packaged sterile again preserve in 2 ℃ of refrigerators standby.
4) preparation stenlizing layer
Select for use silver citrate as the Nano Silver base-material, with its dissolving and formation Nano Silver concentration is the solution of 0.03mol/L, with the bacteria cellulose diaphragm after packaged sterile Kaifeng and be infiltrated in the silver citrate solution, when Nano Silver concentration reaches 0.03mol/L in the material, stop to soak into, diaphragm is obtained the bacteria cellulose stenlizing layer through freeze drying.
5) freeze drying
With the bacteria cellulose diaphragm that does not soak into nano silver-group solution place preserve in the rearmounted sterile bags of-80 ℃ of freeze dryer freeze dryings stand-by.
6) mouth mask preparation
The bacteria cellulose diaphragm that does not soak into nano silver-group solution with drying is that raw material is made the film bag, the bacteria cellulose stenlizing layer that carries nano silver antimicrobials of drying is packed in the film bag, carry out flanging again and be stitched into one, make the mouth mask rope, make air filter type bacterial cellulose mask.
7) sterilization
The air filter type bacterial cellulose mask that makes is put 800 ℃, and 20 atmospheric pressure are handled 1h down, the oxirane sterilization, and the rearmounted 3 ℃ of refrigerators of medical resin container vacuum sealed package are preserved standby.
10) bacteriostasis property test
Choose typical pathogenic microorganisms such as Escherichia coli, staphylococcus aureus, Listeria monocytogenes and do the bactericidal property test
The silver citrate substrate nano silver particle diameter that the present invention adopts is about 20 nanometers, and disinfection effect sees the following form:
| Pathogenic microorganisms | Escherichia coli | Staphylococcus aureus | Listeria monocytogenes | Candida albicans | Salmonella |
| The microorganism fatal rate | 97.89％ | 98.18％ | 96.54％ | 96.17％ | 98.20％ |
Embodiment three
1) type strain
Choose gluconate pyracetobacillus 323 as type strain, it is 5% acetobacter xylinum seed mash that type strain activation is prepared into concentration, acetobacter xylinum seed mash is seeded to makes the initial zymotic fluid that contains the acetobacter xylinum active bacterial strain in the coconut water by fermentation culture medium.
2) fermented and cultured
It is in the culture tank of 30mm that initial zymotic fluid is inserted coconut water by fermentation liquid medium layer height, inserts 28 ℃ constant incubator together with culture vessel and cultivates 2d.Open the oxygen supply valve simultaneously, continuing to feed pressure to the culture tank top is the medical oxygen of 0.075MP, and results thickness is about the bacteria cellulose diaphragm of 1mm.
3) purification process
Above-mentioned bacteria cellulose diaphragm is soaked 1h, 0.1mol chlorohydric acid pickling 0.5h with the 0.5mol hydrochloric acid solution; Soak 1h with the 0.5mol sodium hydroxide solution again; Then soak 1h, wash repeatedly to percentage of admixture＜0.5% with deionized water then with deionized water, be stored in after the ethane via epoxyethane packaged sterile again preserve in 2～4 ℃ of refrigerators standby.
4) preparation stenlizing layer
Select for use silver chlorate as the Nano Silver base-material, with its dissolving and formation Nano Silver concentration is the solution of 0.05mol/L, bacteria cellulose diaphragm after packaged sterile Kaifeng is infiltrated in the nano silver-group solution, when Nano Silver concentration reaches 0.05mol/L in the material, stop to soak into, diaphragm is obtained the bacteria cellulose stenlizing layer through freeze drying.
5) freeze drying
With the bacteria cellulose diaphragm that does not soak into nano silver-group solution place preserve in the rearmounted sterile bags of-80 ℃ of freeze dryer freeze dryings stand-by.
6) mouth mask preparation
The bacteria cellulose diaphragm that does not soak into nano silver-group solution with drying is that raw material is made the film bag, the bacteria cellulose stenlizing layer that carries nano silver antimicrobials of drying is packed in the film bag, carry out flanging again and be stitched into one, make the mouth mask rope, make air filter type bacterial cellulose mask.
7) sterilization
The air filter type bacterial cellulose mask that makes is put 800 ℃, and 20 atmospheric pressure are handled 1h down, the oxirane sterilization, and the rearmounted 3 ℃ of refrigerators of medical resin container vacuum sealed package are preserved standby.
10) bacteriostasis property test
Choose typical pathogenic microorganisms such as Escherichia coli, staphylococcus aureus, Listeria monocytogenes and do the bactericidal property test
The silver chlorate substrate nano silver particle diameter that the present invention adopts is about 20 nanometers, and disinfection effect sees the following form:
| Pathogenic microorganisms | Escherichia coli | Staphylococcus aureus | Listeria monocytogenes | Candida albicans | Salmonella |
| The microorganism fatal rate | 97.15％ | 98.37％ | 96.96％ | 96.06％ | 98.52％ |
In the foregoing description, can adjust parameters such as bacterial classification concentration, fermentation time, the liquid layer degree of depth, oxygen partial pressure according to the variation of each index in the actual mechanical process and set technological parameters such as controlling the raw-material porosity of bacteria cellulose, structural strength and thickness.
Should be pointed out that above only is non-limiting examples of the present invention.Obviously, technical scheme of the present invention is not limited to the foregoing description, and many distortion can also be arranged.All distortion that those of ordinary skill in the art can directly derive or associate from content disclosed by the invention all should be thought protection scope of the present invention.
Claims (7)
1, a kind of air filter type bacterial cellulose mask is characterized in that: be with through the bacteria cellulose of super-dry as the mouth mask frame material through being made.
2, air filter type bacterial cellulose mask according to claim 1, it is characterized in that: described mouth mask is to sew up and make by carrying out flanging after compound through cryodesiccated three layers of bacteria cellulose diaphragm, wherein two skins are the bacteria cellulose filter course, and the intermediate layer is the bacteria cellulose stenlizing layer; Described bacteria cellulose stenlizing layer is to be infiltrated on nano silver-group solution after freeze drying and making by the bacteria cellulose diaphragm; Described nano silver-group solution is meant and the Nano Silver base-material is dissolved in the water to form concentration be the solution of 0.001～0.050mol/L; Described Nano Silver base-material is meant silver nitrate, silver chlorate, silver citrate or silver oxalate.
3, air filter type bacterial cellulose mask according to claim 2 is characterized in that: the thickness of described bacteria cellulose layer is 0.1～3.0mm.
4, the production method of the described air filter type bacterial cellulose mask of a kind of claim 2, it is characterized in that: concrete steps are as follows:
1), apolegamy bacterial classification
Choose the cellulosic bacterial classification of energy secreting bacteria as type strain, the type strain activation is prepared into the seed mash, throw in the seed mash then and to fermentation medium, make the initial zymotic fluid that contains active bacterial strain;
2), logical aerobe fermentation
The initial zymotic fluid that will contain active bacterial strain is inserted in the culture tank, feeds oxygen-enriched air in liquid surface, makes the acetobacter xylinum that is positioned at the gas-liquid two-phase interface breed the justacrine bacteria cellulose in a large number, obtains the bacteria cellulose diaphragm;
3), purification process
The bacteria cellulose diaphragm that makes is spent deionised water respectively to percentage of admixture＜0.5% behind acid solution, dipping by lye, after packaged sterile, be stored in again in 2～4 ℃ of refrigerators preserve standby;
4), preparation stenlizing layer
The Nano Silver base-material is dissolved in the water, and to form Nano Silver concentration be the nano silver-group solution of 0.001～0.050mol/L, to be infiltrated in the nano silver-group solution behind the Kaifeng of the bacteria cellulose diaphragm after the packaged sterile again, when Nano Silver concentration reaches 0.001～0.050mol/L in the diaphragm, stop to soak into, diaphragm is obtained the bacteria cellulose stenlizing layer through freeze drying.Described Nano Silver base-material is meant silver nitrate, silver chlorate, silver citrate or silver oxalate;
5), drying
Carry out preserving in the rearmounted sterile bag of freeze drying the bacteria cellulose diaphragm that does not soak into nano silver-group solution stand-by;
6), mouth mask preparation
Will be compound through cryodesiccated two layers of bacteria cellulose diaphragm and one deck bacteria cellulose stenlizing layer be three layers, wherein two skins are the bacteria cellulose diaphragm, the intermediate layer is the bacteria cellulose stenlizing layer, carry out flanging then and be stitched into one, make the mouth mask rope, make air filter type bacterial cellulose mask.
5, the production method of air filter type bacterial cellulose mask according to claim 4 is characterized in that: described bacterial classification is that acetobacter xylinum belongs to bacterial strain.
6, the production method of air filter type bacterial cellulose mask according to claim 4 is characterized in that: it is gluconate pyracetobacillus 323 that described acetobacter xylinum belongs to bacterial strain.
7, the production method of air filter type bacterial cellulose mask according to claim 4 is characterized in that: the concentration of bacterial strain is 0.5%～95% in the described seed mash.
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