CN104982516B - A kind of the green ecological preservation method and antistaling agent of citrus - Google Patents

A kind of the green ecological preservation method and antistaling agent of citrus Download PDF

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CN104982516B
CN104982516B CN201510331130.8A CN201510331130A CN104982516B CN 104982516 B CN104982516 B CN 104982516B CN 201510331130 A CN201510331130 A CN 201510331130A CN 104982516 B CN104982516 B CN 104982516B
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fruit
citrus
bacillus subtilis
fresh
fruits
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CN104982516A (en
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吴日章
袁永雁
王勇
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Zhenluse Technology Co Ltd Zhuhai City
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Zhenluse Technology Co Ltd Zhuhai City
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Abstract

The present invention relates to the green ecological preservation method and antistaling agent of a kind of citrus, belongs to and adopt rear fruits and vegetables preservation field.Fruit is used in screening storage first;It is immersed in the solution 1-2min disinfection treatment containing polyaminopropyl biguanide;By the citrus fruit after disinfection treatment, it is immersed in the biological preservation liquid of the R31 containing bacillus subtilis and handles 1-2min;Individual packing is carried out to fruit with Polythene Bag.Utilize the fresh-keeping citrus of this method, it can reach the fresh-keeping purpose of green ecological of citrus, guaranteeing effect suitable with chemical bactericide, the rotting rate of citrus is reduced, fruit quality is kept and prolongs storage period, guarantees to avoid, reduction residual fresh-keeping using chemical sterilization medicament while fruit commodity value improves fresh-keeping commodity market competitiveness, reduce food safety risk, reduce chemical bactericide to use, reduces the influence to ecology, environment, health green, ecological, environmental protective.This method is easy to industrialized production and promotes and applies, and has good market promotion prospect.

Description

A kind of the green ecological preservation method and antistaling agent of citrus
Technical field
The invention belongs to adopt rear fruits and vegetables preservation field, it is related to that citrus is fresh-keeping, in particular to the green ecological of a kind of citrus is protected Fresh method and antistaling agent.
Background technique
Citrus is the second largest fruit in China, and yield is big, and distribution is wide, and postharvest decay problem is serious, affects citrus industry It develops in a healthy way.Citrus postharvest decay is because pathologic disease and physiological disturbance cause.Physiological disturbance have damage to plants caused by sudden drop in temperature, oil vacuole disease, The diseases such as brown spot, oedema and dry scar.Pathologic disease mainly includes penicilliosis, green mould, black rot, blossom-end rot (packet It is rotten to include brown base of a fruit corruption and the black base of a fruit), sour rot and anthracnose etc..
Adopt the fresh-keeping means of rear citrus storage at present, with chemical bactericide it is fresh-keeping based on, main ingredient have Prochloraz, suppression it is mould Azoles, bellkute, probenazole etc., technology also stay in the chemical sterilization fresh-keeping stage.These compositions itself are also to adopt preceding cultivation of fruit tree The common drug of phase uses throughout the year, generates resistance, preventive effect decline;It is re-used as post-harvest fresh-keeping medication, residual quantity is a problem;It protects The safety of citrus is relatively difficult to guarantee after fresh, and there are food safety hazards;The waste water being discharged after use influences ecology, environment.
The fresh-keeping industry of citrus also has and the way that sodium hypochlorite is used as disinfection, such as Chinese patent application is added in wash phase Number: it mentions in technical solution disclosed in 201410242839.6 and being carried out disinfection processing using sodium hypochlorite.Sodium hypochlorite is unstable, Light-exposed easy decomposition has chlorine flavor, uses in freezer, will affect indoor air quality;There is sensitization, contacted through common hand, Palm is largely perspired, and nail is thinning, the adverse effect such as trichomadesis, in disinfecting process, human body may be often in contact with disinfection In the environment of liquid, sodium hypochlorite is not a good selection.
Polyaminopropyl biguanide is one kind of biguanides, molecular formula: (C5H14N6) X, it is a kind of high-efficiency broad spectrum safety Mould proof fungi-proofing substance.Substance is stablized, and the characteristic of cation superpolymer makes it optionally be adsorbed in pathogenic microorganisms surface, Cell membrane is acted on, film and eucaryotic cell structure are destroyed.Research shows that polyaminopropyl biguanide belongs to practical nontoxic grade, polyurethane/cationic third Base biguanides is there are also purifying water body effect, and the polyaminopropyl biguanide of 3-4ppm is perfectly safe to aquatile, to environment without dirt Dye, energy is natural or by biological decomposition, can be by microbial digestion catabolism after high dilution.Data at home and abroad report, poly- ammonia Propyl biguanides be mainly used in cosmetics, medicine, food processing surface sterilization, separately have number of patent application 201010542279.8 Biguanides are used for citrus disease prevention and treatment.Disclose 0.05%~0.6% hexamethylene and 0.05% ~0.2% imidazoles fungicide, benzimidazole germicide compounding antistaling agent, i.e., with imidazoles fungicide, benzimidazole Bactericide compounded, technology still stays in the chemical sterilization fresh-keeping stage, inevitably exist chemical bactericide it is fresh-keeping it is unfavorable it Place.
Bacillus subtillis is prevalent in soil, in fruits and vegetables and the surface of plant and tissue, no pollution to the environment, to people It is nontoxic, harmless with animal.It is more in the feasibility of disease control, research and the relevant report of application about Bacillus subtillis, Research especially for disease prevention and control during planting, Related product (" the standing grain clear water township of such as Wuhan Tian favour of industrialization (100,000,000,000 spores/gram bacillus subtilis ");Report such as Zhan's happiness, history phoenix jade etc. of fruit post-harvest fresh-keeping application, Yang Zhen etc., The research of Zhao Yan etc., relate generally to Bacillus subtillis to adopt the control efficiency of rear fruit disease, to the fresh-keeping i.e. quality of fruit Holding effect, the research of biocontrol mechanism, especially banana, pears, grape, tomato post-harvest fresh-keeping, show Ko subtilis bar Pseudomonas bacterium is for adopting rear fruit disease prevention and treatment and fresh-keeping feasibility.
101870959 B of Chinese invention patent CN is further centrifuged using the fermentation liquid that fermentation of bacillus subtilis obtains After go supernatant to obtain wet thallus, wet thallus with etc. the calcium chloride of weight be uniformly mixed, be dried to powdered, obtain dry bacteria, Every gram of dry bacteria has 1 × 1011A gemma is used for black rot of pear, grape grey mould, watermelon blight.Relate to original powder (dry powder Microbial inoculum) production, but original powder directly uses that there are the following problems: when (i) diluting, dissolubility is poor in water;(ii) after diluting, bacterium It is unprotected, it is affected by external environmental condition, such as the effect of light, influences the performance of bacterium fresh-keeping effect;(iii) the adhesion of original powder Property it is poor, be unfavorable for bacterium powder crop and fruit surface, reduce bacterium in crop and the effective content of fruit surface, reduce fresh-keeping effect.
In summary the fresh-keeping industry technology status of citrus storage, from effectively reduce after fruit is adopted lose, reduce chemical sterilization Usage amount improves the foodsafety of fresh-keeping fruit, solves medicament residue problem, changes the fresh-keeping Industry of citrus, opens The exploration of citrus green ecological preservation method is opened up.
Therefore it provides a kind of rotting rate that can effectively reduce citrus, keep fruit quality and prolonging storage period, green, peace Entirely, it is difficult just to become technical field technology urgently to be solved for the prevention and treatment of environmentally friendly citrus disease, the method for preservation and freshness and antistaling agent Topic.
Summary of the invention
The first purpose of this invention is to provide a kind of rotting rate that can effectively reduce citrus, keeps fruit quality and prolong Long freshness date, green, safe and environment-friendly citrus disease prevention and treatment, preservation and freshness method.
The purpose of foregoing invention of the invention, reaches by the following technical programs:
A kind of green ecological preservation method of citrus, its step are as follows:
(1) fruit is used in screening storage: selecting without mechanical wounding, no disease and pests harm, without rotten citrus fruit, as fresh-retaining preserving With fruit, cleaning is for use;
(2) it disinfects: by citrus fruit stand-by obtained by step (1), being immersed in polyaminopropyl biguanide disinfection anticorrosion dissolving Liquid is handled 1-2 minutes, to remove fresh citrus fruit remained on surface dirt, surface pathogenic microorganisms, act on the latent cause in part Germ, it is drained and standby;
(3) biological preservation is handled: the citrus fruit after disinfection treatment obtained by step (2) is immersed in containing bacillus subtilis The biological preservation liquid of bacterium R31 is handled 1-2 minutes, and processing is placed in basket, is placed in disinfection storehouse ventilation airing;
(4) packing processes: step (3) treated citrus fruit chooses machinery wound rotten fruit after placing 3-5 days, and with gathering Vinyl bag individual packing is stored after filling basket in suitable condition, such as room temperature storage or refrigeration or the storage of ventilation library.
The disinfection listerine of polyaminopropyl biguanide described in the step (2) is produced by Zhuhai Zhenlvse Technology Co., Ltd 40 weight % polyaminopropyl biguanide aqua product, diluted by 500 times~1000 multiples.
The specific formula of the polyaminopropyl biguanide aqua of the 40 weight % is (weight ratio): polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
The biological preservation liquid of the R31 containing bacillus subtilis described in the step (3) by the R31 containing bacillus subtilis mandarin orange Tangerine bio-preservative is diluted by 200 times~800 times multiples.
Second goal of the invention of the invention is to provide the citrus bio-preservative of R31 containing bacillus subtilis a kind of.
The purpose of foregoing invention of the invention, reaches by the following technical programs:
1, the preparation method of bacillus subtilis R31 original powder, includes the following steps:
(1) activated spawn
Activate bacillus subtilis R31 bacterial strain (bacillus subtilis R31, it is characterized in that " patent CN 101760438B institute Patented strain " bacillus subtilis R31 " in the broad-spectrum antifungal plant endophytic bacillus subtillis stated and its application "), To bacterium colony milky of the bacillus subtilis R31 bacterial strain on NA solid medium, the tiny circle of bacterium colony, surface is smooth sticky, Without obvious protrusion, partial folds;
(2) preparation of primary seed solution
It uses NA fluid nutrient medium as primary-seed medium, chooses the single colonie on NA solid medium, be inoculated in one In grade seed flask, first order seed shaking flask is cultivated 9 hours at 35 DEG C, 200r/min, obtains primary seed solution;
(3) preparation of secondary seed solution
Culture medium in seeding tank is still NA fluid nutrient medium, and at 121 DEG C, sterilize 20min, and primary seed solution is accessed kind It is cultivated in sub- tank, secondary seed solution is obtained at the end of culture;
(4) bacillus subtilis R31 ferments
By the secondary seed solution of bacillus subtilis R31 obtained by step (3), accessed in fermentor according to inoculum concentration for 1%, It is cultivated under fermentation medium, obtains zymocyte liquid (predominantly gemma);
(5) bacillus subtilis R31 bacterium original powder produces
Step (4) resulting zymocyte liquid is added precipitated calcium carbonate, makes concentrated fermenting liquid after disk plate centrifuge is concentrated Weight ratio with precipitated calcium carbonate is 100:3, obtains liquid-solid mixture, after which is stirred 35min, utilizes conveying Liquid-solid mixture is sent into drying in atomizer tower by pump, obtains bacillus subtilis R31 bacterium original powder.
NA culture medium prescription (g/L) described in the step (1): peptone 5g, beef extract 3g, glucose 2.5g, agar 15g, solvent are water, 1000mL, pH7.2, and 1.5% agar is not added in fluid nutrient medium;
The inoculum concentration of seeding tank described in the step (3) is 1% (V/V);Condition of culture are as follows: ventilation ratio 1:1, stirring turn Speed is 200r/min, and 35 DEG C of cultivation temperature, tank presses 0.05Mpa, is cultivated 11 hours;The viable bacteria content of the secondary seed solution is 2- 6×108Cfu/mL, OD value are greater than 0.85;
The formula (weight percent) of fermentation medium described in the step (4): cornstarch 2%, bean cake powder 1.5%, yeast extract 0.8%, sodium chloride 0.25%, magnesium sulfate 0.4%, dipotassium hydrogen phosphate 0.3%, pH7.2,121 DEG C sterilizing 30min;Condition of culture are as follows: ventilation ratio 1:0.7, speed of agitator 200r/min, tank pressure 0.05MPa, 35 DEG C of cultivation temperature, training The time is supported as 48h, defoaming agent is added according to foam situation in fermentation process;The lower tank time will be discharged with sampling 90% or more microscopy gemma Subject to, the living spores content of bacillus subtilis R31 bacterium original powder is 40~60 × 10 in fermentation liquid8cfu/mL;
The drying condition of atomizer tower described in the step (5) are as follows: intake air temperature is 180-200 DEG C, outlet air Mouth temperature is 80-85 DEG C, and water content is greater than 2000 × 10 less than 5% (W/W), living spores content in bacterium original powder8cfu/g。
2, the citrus bio-preservative of a kind of R31 containing bacillus subtilis, product forms are that wettable powder or oil are outstanding Floating agent.
A kind of preparation of the citrus bio-preservative wettable powder of the R31 containing bacillus subtilis, its step are as follows:
(1) bacillus subtilis R31 original powder, chitosan oligosaccharide, wetting agent, dispersing agent, a certain amount of carrier, in high speed ten thousand are weighed Co-grinding 1.5 hours in energy pulverizer;
(2) on the basis of step (1), stabilizer is added, and supply carrier, continues to crush 1 hour, obtains containing bacillus subtilis The wettable powder of bacterium R31 living spores.
The component and proportion of the citrus bio-preservative wettable powder of gained R31 containing bacillus subtilis are as follows:
The wetting agent, dispersing agent, stabilizer, carrier are conventional surfactant.
The wetting agent is fatty alcohol polyoxyethylene ether, pull open powder, lauryl alcohol polyethylene glycol oxide base ether sodium sulfate, dodecane One of base benzene sulfonic acid sodium salt, calcium dodecyl benzene sulfonate, sldium lauryl sulfate and ethoxylated dodecyl alcohol sodium sulfonate are several The mixing of kind arbitrary proportion.
The dispersing agent is dispersing agent MF, lignosulfonates, naphthalenesulfonic acid-formaldehyde condensate (NN0), polyethylene pyrrole The mixing of one or more of pyrrolidone, polyethylene glycol and polyvinyl alcohol arbitrary proportion.
The stabilizer is one or more of sucrose, sodium chloride, potassium sorbate and ascorbic acid arbitrary proportion Mixing.
The mixing of the carrier such as one or more of precipitated calcium carbonate, kaolin and diatomite arbitrary proportion.
A kind of preparation of the citrus bio-preservative oil-suspending agent of the R31 containing bacillus subtilis, its step are as follows:
(1) quantitative bacillus subtilis R31 original powder is weighed, chitosan oligosaccharide, Component Vectors, emulsifier mixes in mixing kettle Close stirring 2 hours;
(2) stabilizer is added into the mixing kettle after step (1) stirring 2 hours, and supplies carrier to 100%, continue to stir It mixes 0.5 hour, the oil-suspending agent of the stable living spores of R31 containing bacillus subtilis can be formed.
The component and proportion of the citrus bio-preservative oil-suspending agent of gained R31 containing bacillus subtilis are as follows:
The floating agent stabilizer, carrier are conventional surfactant or chemical component.
The emulsifier is polyoxyethylene sorbitol acid anhydride monopalmitate (polysorbate40), polyoxyethylene sorbitol acid anhydride list is hard Resin acid ester (polysorbate60), Tween-80 (Tween 80), fatty alcohol polyoxyethylene ether, oleic alcohol polyoxyethylene The mixing of one or more of ether and cithrol arbitrary proportion.
The stabilizer is one or more of any ratios in potassium sorbate, sodium benzoate, sodium chloride, sucrose and sodium acetate The mixing of example.
One or more of arbitrary proportions of the carrier such as soybean oil, rapeseed oil, epoxidized soybean oil and corn oil etc. Mixing.
The present invention has following advantages and effect:
The present invention is the preservation method of a kind of citrus green, ecology, and disinfection used, bio-preservative are all practical nontoxic Substance, nontoxic, safe to people, animal and environment, whole fresh-keeping effect simultaneously, improves fresh-keeping fruit after guaranteeing to adopt citrus Foodsafety, solve medicament residue problem, reduce chemical sterilization medicament and use, change the fresh-keeping Industry of citrus;Subtract Influence of the small waste water to ecology, environment, treated, and water also helps soil disease prevention and treatment, purifies water, free from environmental pollution, ring It protects, ecology;Ensure that citrus green ecological preservation method of the invention achievees the purpose that food safety, environmental-friendly, green ecological.
The preservation method of citrus green, ecology of the invention, have obvious characteristic: certain density polyaminopropyl biguanide is to mandarin orange Tangerine storage disease has broad-spectrum antibacterial, can effectively clear fruit surface microorganism, plays detoxicating to the microorganism on surface and wound, removes Bacterium effect, and can partial action in the germ of latent dip dyeing, reduce the generation of disease, but weaker to physiology quality regulating and controlling effect, The easy deterioration of anaphase storage physiology, Disease management is not good enough.Bacillus subtilis R31 be existing patented strain (patent No.: 200910214430.2), having the active bacterial strain of broad-spectrum disease resistance fungal pathogens, experiment shows the validity that disease is infected to citrus, The induced resistance and secondary metabolite of bacterium can effectively adjust citrus post-harvest physiology, improve fruit disease resistance, physiology is delayed to decline Always, storage quality is kept, guarantees storage and shelf life, but the anti-microbial inoculum control efficiency of biology need further to be promoted, and is stored Environment influences big.
The method of the present invention makes full use of complementation between the two, using sterilizing that most of germ is clear to the greatest extent It removes, bacteria agent is recycled to control remaining pathogenic bacteria, induced resistance, physiological regulatory action improve fruit disease resistance, delay Physiological aging keeps storage quality.Poly- ammonia third a small amount of using the effect degradation of biological bacteria secondary metabolites and bacterium itself simultaneously Base biguanides reduces chemical substance in the residual in fruit face.Therefore it can effectively reduce rotting for citrus using the method for the present invention Citrus Fruits In Storage Rate keeps fruit quality and prolongs storage period, and guarantees fruit commodity value, the safety of food, green character improve fresh-keeping commodity The market competitiveness.
Below by specific embodiment, the present invention will be further described, but is not meant to the scope of the present invention Limitation.
Specific embodiment
Reference examples 1
It is Germicidal efficacy holding conditions based on room temperature: 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%.
It selects without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine, as fresh-retaining preserving with fruit, cleaning is stand-by.
(1) by stand-by granulated sugar tangerine, 500 times of dilute solutions processing 1min of 25% Prochloraz, place Preservation Treatment: are immersed in Vacant room after being placed in disinfection after reason, windowing ventilation.
(2) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(3) result: it is shown in Table 1.
The 25% Prochloraz missible oil, is produced by Zhuhai Zhenlvse Technology Co., Ltd.
Embodiment 1
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) cleaning and sterilizing is handled: by stand-by granulated sugar tangerine, being immersed in 500 times of dilutions of 40 weight % polyaminopropyl biguanides Solution is handled 2 minutes, is removed fresh fruit remained on surface dirt, surface pathogenic microorganisms, is acted on the latent pathogenic bacteria in part, drip It does spare;
(3) biological preservation is handled: by the granulated sugar tangerine after disinfection treatment, being immersed in 70% bacillus subtilis R31 biology guarantor Fresh dose of 200 times of dilute solutions are handled 1 minute, the vacant room after disinfection is placed in after processing, windowing ventilation;
(4) packing processes: choosing machinery wound rotten fruit after 3 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(5) result: it is shown in Table 1.
The formula (weight ratio) of the 40% polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, Excess water.
The citrus bio-preservative of the 70% bacillus subtilis R31 is every gram containing 1.4 × 1011The withered grass bud of cfu The microbial bacterial agent product of spore bacillus R31 gemma.
1, the preparation method of bacillus subtilis R31 original powder, includes the following steps:
(1), activated spawn
Activate bacillus subtilis R31 bacterial strain (bacillus subtilis R31, it is characterized in that " patent CN 101760438B institute Patented strain " bacillus subtilis R31 " in the broad-spectrum antifungal plant endophytic bacillus subtillis stated and its application "), To bacterium colony milky of the bacillus subtilis R31 bacterial strain on NA solid medium, the tiny circle of bacterium colony, surface is smooth sticky, Without obvious protrusion, partial folds;
(2), the preparation of primary seed solution
It uses NA fluid nutrient medium as primary-seed medium, chooses the single colonie on NA solid medium, be inoculated in one In grade seed flask, first order seed shaking flask is cultivated 9 hours at 35 DEG C, 200r/min, obtains primary seed solution;
(3), the preparation of secondary seed solution
Culture medium in seeding tank is still NA fluid nutrient medium, and at 121 DEG C, sterilize 20min, and primary seed solution is accessed kind It is cultivated in sub- tank, secondary seed solution is obtained at the end of culture;
(4), bacillus subtilis R31 ferments
By the secondary seed solution of bacillus subtilis R31 obtained by step (3), accessed in fermentor according to inoculum concentration for 1%, It is cultivated under fermentation medium, obtains zymocyte liquid (predominantly gemma);
(5), bacillus subtilis R31 bacterium original powder produces
Step (4) resulting zymocyte liquid is added precipitated calcium carbonate, makes concentrated fermenting liquid after disk plate centrifuge is concentrated Weight ratio with precipitated calcium carbonate is 100:3, obtains liquid-solid mixture, after which is stirred 35min, utilizes conveying Liquid-solid mixture is sent into drying in atomizer tower by pump, obtains bacillus subtilis R31 bacterium original powder.
NA culture medium prescription (g/L) described in the step (1): peptone 5g, beef extract 3g, glucose 2.5g, agar 15g, solvent are water, 1000mL, pH7.2, and 1.5% agar is not added in fluid nutrient medium;
The inoculum concentration of seeding tank described in the step (3) is 1% (V/V);Condition of culture are as follows: ventilation ratio 1:1, stirring turn Speed is 200r/min, and 35 DEG C of cultivation temperature, tank presses 0.05Mpa, is cultivated 11 hours;The viable bacteria content of the secondary seed solution is 2- 6×108Cfu/mL, OD value are greater than 0.85;
The formula (weight percent) of fermentation medium described in the step (4): cornstarch 2%, bean cake powder 1.5%, yeast extract 0.8%, sodium chloride 0.25%, magnesium sulfate 0.4%, dipotassium hydrogen phosphate 0.3%, pH7.2,121 DEG C sterilizing 30min;Condition of culture are as follows: ventilation ratio 1:0.7, speed of agitator 200r/min, tank pressure 0.05MPa, 35 DEG C of cultivation temperature, training The time is supported as 48h, defoaming agent is added according to foam situation in fermentation process;The lower tank time will be discharged with sampling 90% or more microscopy gemma Subject to, the living spores content of bacillus subtilis R31 bacterium original powder is 40~60 × 10 in fermentation liquid8cfu/mL;
The drying condition of atomizer tower described in the step (5) are as follows: intake air temperature is 180-200 DEG C, outlet air Mouth temperature is 80-85 DEG C, and water content is greater than 2000 × 10 less than 5% (W/W), living spores content in bacterium original powder8cfu/g。
A kind of preparation of the citrus bio-preservative wettable powder of the R31 containing bacillus subtilis, its step are as follows:
(1) bacillus subtilis R31 original powder, chitosan oligosaccharide, such as calcium dodecyl benzene sulfonate, lignin sulfonic acid are weighed in proportion Salt, diffusant (MF), co-grinding 1.5 hours in high speed Universal pulverizer;
(2) on the basis of step (1), sucrose, ascorbic acid is added, and supply precipitated calcium carbonate, continues to crush 1 hour, The Microbial wettable powder of bacillus subtilis R31 living spores must be contained.
It is formed and proportion is as follows:
(1) 70 grams of bacillus subtilis R31 original powder is weighed, 10 grams of chitosan oligosaccharide, 2 grams of calcium dodecyl benzene sulfonate, lignin 2.5 grams of sulfonate, 2 grams of dispersing agent MF, co-grinding 1.5 hours in high speed Universal pulverizer;
(2) on the basis of step (1), 1 gram of sucrose of addition, 2 grams of ascorbic acid, and 10.5 grams of precipitated calcium carbonate are supplied, after Continued powder broken 1 hour, obtain the Microbial wettable powder of the living spores of R31 containing bacillus subtilis.
Reference examples 2
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) cleaning and sterilizing is handled: by stand-by granulated sugar tangerine, being immersed in 500 times of dilutions of 40 weight % polyaminopropyl biguanides Solution is handled 2 minutes, is removed fresh fruit remained on surface dirt, surface pathogenic microorganisms, is acted on the latent pathogenic bacteria in part, drip It is dry, ventilation;
(3) packing processes: choosing machinery wound rotten fruit after 3 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(4) result: it is shown in Table 1.
The formula (weight ratio) of the 40% polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, Excess water.
Reference examples 3
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) biological preservation is handled: by stand-by granulated sugar tangerine, being immersed in 200 times of 70% bacillus subtilis R31 antistaling agent Dilute solution is handled 1 minute, the vacant room after disinfection is placed in after processing, windowing ventilation;
(3) packing processes: choosing machinery wound rotten fruit after 3 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(4) result: it is shown in Table 1.
The citrus bio-preservative of the 70% bacillus subtilis R31 is every gram containing 1.4 × 1011The withered grass bud of cfu The microbial bacterial agent product of spore bacillus R31 gemma.
Reach particular by following technical scheme: for 100 grams of total weight, composition and proportion are as follows:
(1) 70 grams of bacillus subtilis R31 original powder is weighed, 10 grams of chitosan oligosaccharide, 2 grams of calcium dodecyl benzene sulfonate, lignin 2.5 grams of sulfonate, 2 grams of dispersing agent MF, co-grinding 1.5 hours in high speed Universal pulverizer;
(2) on the basis of step (1), 1 gram of sucrose of addition, 2 grams of ascorbic acid, and 10.5 grams of precipitated calcium carbonate are supplied, after Continued powder broken 1 hour, obtain the Microbial wettable powder of the living spores of R31 containing bacillus subtilis.
Embodiment 2
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) cleaning and sterilizing is handled: by stand-by granulated sugar tangerine, being immersed in 1000 times of dilute solutions of 40% polyaminopropyl biguanide Processing 1-2 minutes removes fresh fruit remained on surface dirt, surface pathogenic microorganisms, acts on the latent pathogenic bacteria in part, drains It is spare;
(3) biological preservation is handled: by the granulated sugar tangerine after disinfection treatment, being immersed in 10% bacillus subtilis R31 biology guarantor Fresh dose of 1000 times of dilute solutions are handled 1 minute, the vacant room after disinfection is placed in after processing, windowing ventilation;
(4) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(5) result: it is shown in Table 1.
The formula (weight ratio) of the 40% polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, Excess water.
The citrus bio-preservative of the 10% bacillus subtilis R31 is every gram containing 2 × 1010The withered grass gemma of cfu The microbial bacterial agent product of bacillus R31 gemma.
The formula of the citrus bio-preservative of 10% bacillus subtilis R31 of the invention is as follows:
10 grams of bacillus subtilis R31 original powder (preparation method is same as Example 1)
(1) 10 grams of bacillus subtilis R31 original powder is weighed, 10 grams of chitosan oligosaccharide, 4 grams of fatty alcohol polyoxyethylene ether, pull open powder 2 Gram, 3 grams of polyvinylpyrrolidone, co-grinding 1.5 hours in high speed Universal pulverizer;
(2) on the basis of step (1), it is added 1 gram of sodium chloride, and supply 70 grams of precipitated calcium carbonate, continues to crush 1 hour, The Microbial wettable powder of bacillus subtilis R31 living spores must be contained.
Reference examples 4
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) cleaning and sterilizing is handled: by stand-by granulated sugar tangerine, being immersed in 1000 times of dilute solutions of 40% polyaminopropyl biguanide 1-2min is handled, fresh fruit remained on surface dirt, surface pathogenic microorganisms is removed, acts on the latent pathogenic bacteria in part, drain, Ventilation;
(3) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(4) result: it is shown in Table 1.
The formula (weight ratio) of the 40% polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, Excess water.
Reference examples 5
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) biological preservation is handled: by stand-by granulated sugar tangerine, being immersed in 10% bacillus subtilis R31 bio-preservative 1000 times of dilute solutions handle 1min, the vacant room after disinfection is placed in after processing, windowing ventilation;
(3) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(4) result: it is shown in Table 1.
The citrus bio-preservative of the 10% bacillus subtilis R31 is every gram containing 2 × 1010The withered grass gemma of cfu The microbial bacterial agent product of bacillus R31 gemma.
The formula of the citrus bio-preservative of 10% bacillus subtilis R31 of the invention is as follows:
(1) 10 grams of bacillus subtilis R31 original powder is weighed, 70 grams of chitosan oligosaccharide, 4 grams of fatty alcohol polyoxyethylene ether, pull open powder 2 Gram, 3 grams of polyvinylpyrrolidone, co-grinding 1.5 hours in high speed Universal pulverizer;
(2) on the basis of step (1), it is added 1 gram of sodium chloride, and supply 10 grams of precipitated calcium carbonate, continues to crush 1 hour, The Microbial wettable powder of bacillus subtilis R31 living spores must be contained.
Embodiment 3
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) cleaning and sterilizing is handled: by stand-by granulated sugar tangerine, being immersed in 800 times of dilute solutions of 40% polyaminopropyl biguanide 1min is handled, fresh fruit remained on surface dirt, surface pathogenic microorganisms is removed, acts on the latent pathogenic bacteria in part, drain standby With;
(3) biological preservation is handled: by the granulated sugar tangerine after disinfection treatment, being immersed in 50% bacillus subtilis R31 biology guarantor Fresh dose of 800 times of dilute solutions handle 1min, the vacant room after disinfection is placed in after processing, windowing ventilation;
(4) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(5) result: it is shown in Table 1.
The formula (weight ratio) of the 40% polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, Excess water.
The citrus bio-preservative of the 50% bacillus subtilis R31 is every gram containing 1 × 1011The withered grass gemma of cfu The microbial bacterial agent product of bacillus R31 gemma.
The composition and proportion of the citrus bio-preservative of 50% bacillus subtilis R31 of the invention are as follows:
(1) 50 grams of bacillus subtilis R31 original powder is weighed, 25 grams of chitosan oligosaccharide, 4 grams of neopelex, NN01 grams, 2 grams of lignosulfonates, co-grinding 2 hours in high speed Universal pulverizer;
(2) on the basis of step (1), it is added 1 gram of potassium sorbate, and supply 16.5 grams of kaolin, continues to crush 1 hour, The Microbial wettable powder of bacillus subtilis R31 living spores must be contained.
Reference examples 6
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) cleaning and sterilizing is handled: by stand-by granulated sugar tangerine, being immersed in 800 times of dilute solutions of 40% polyaminopropyl biguanide 1min is handled, fresh fruit remained on surface dirt, surface pathogenic microorganisms is removed, acts on the latent pathogenic bacteria in part, drain, lead to Wind;
(3) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(4) result: it is shown in Table 1.
The formula (weight ratio) of the 40% polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, Excess water.
Reference examples 7
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) biological preservation is handled: by stand-by granulated sugar tangerine, being immersed in 800 times of 25% bacillus subtilis R31 antistaling agent Dilute solution handles 1min, the vacant room after disinfection is placed in after processing, windowing ventilation;
(3) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(4) result: it is shown in Table 1.
The citrus bio-preservative of the 50% bacillus subtilis R31 is every gram containing 1 × 1011The withered grass gemma of cfu The microbial bacterial agent product of bacillus R31 gemma.
The composition and proportion of the citrus bio-preservative of 50% bacillus subtilis R31 of the invention are as follows:
(1) 50 grams of bacillus subtilis R31 original powder is weighed, 25 grams of chitosan oligosaccharide, 4 grams of neopelex, NN01 grams, 2 grams of lignosulfonates, co-grinding 2 hours in high speed Universal pulverizer;
(2) on the basis of step (1), it is added 1 gram of potassium sorbate, and supply 16.5 grams of kaolin, continues to crush 1 hour, The Microbial wettable powder of bacillus subtilis R31 living spores must be contained.
Embodiment 4
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) cleaning and sterilizing is handled: by stand-by granulated sugar tangerine, being immersed in 600 times of dilute solutions of 40% polyaminopropyl biguanide 1min is handled, fresh fruit remained on surface dirt, surface pathogenic microorganisms is removed, acts on the latent pathogenic bacteria in part, drain standby With;
(3) biological preservation is handled: by the granulated sugar tangerine after disinfection treatment, being immersed in 30% bacillus subtilis R31 biology guarantor Fresh dose of 600 times of dilute solutions handle 1min, the vacant room after disinfection is placed in after processing, windowing ventilation;
(4) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(5) result: it is shown in Table 1.
The formula (weight ratio) of the 40% polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, Excess water.
The citrus bio-preservative of the 30% bacillus subtilis R31 is every gram containing 6 × 1010The withered grass gemma of cfu The microbial bacterial agent product of bacillus R31 gemma.
The composition and proportion of the citrus bio-preservative of 30% bacillus subtilis R31 of the invention are as follows:
(1) 30 grams of bacillus subtilis R31 original powder is weighed, 15 grams of chitosan oligosaccharide, 20 grams of soybean oil, fatty alcohol polyoxyethylene ether It 12 grams, 8 grams of cithrol, is mixed 2 hours in mixing kettle;
(2) 1 gram of sodium benzoate of the addition into the mixing kettle after step (1) stirring 2 hours, 3 grams of sodium chloride, 0.5 gram of sucrose, And soybean oil is supplied to 100%, continue stirring 0.5 hour, the oil of the stable living spores of R31 containing bacillus subtilis can be formed Suspending agent.
Reference examples 8
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) cleaning and sterilizing is handled: by stand-by granulated sugar tangerine, being immersed in 600 times of dilute solutions of 40% polyaminopropyl biguanide 1min is handled, fresh fruit remained on surface dirt, surface pathogenic microorganisms is removed, acts on the latent pathogenic bacteria in part, drain, lead to Wind;
(3) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(4) result: it is shown in Table 1.
The formula (weight ratio) of the 40% polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, Excess water.
Reference examples 9
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) biological preservation is handled: by stand-by granulated sugar tangerine, being immersed in 600 times of 30% bacillus subtilis R31 antistaling agent Dilute solution handles 1min, the vacant room after disinfection is placed in after processing, windowing ventilation;
(3) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(4) result: it is shown in Table 1.
The citrus bio-preservative of the 30% bacillus subtilis R31 is every gram containing 6 × 1010The withered grass gemma of cfu The microbial bacterial agent product of bacillus R31 gemma.
The composition and proportion of the citrus bio-preservative of 30% bacillus subtilis R31 of the invention are as follows:
(1) 30 grams of bacillus subtilis R31 original powder is weighed, 15 grams of chitosan oligosaccharide, 20 grams of soybean oil, fatty alcohol polyoxyethylene ether It 12 grams, 8 grams of cithrol, is mixed 2 hours in mixing kettle;
(2) 1 gram of sodium benzoate of the addition into the mixing kettle after step (1) stirring 2 hours, 3 grams of sodium chloride, 0.5 gram of sucrose, And soybean oil is supplied to 100%, continue stirring 0.5 hour, the oil of the stable living spores of R31 containing bacillus subtilis can be formed Suspending agent.
Embodiment 5
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) cleaning and sterilizing is handled: by stand-by granulated sugar tangerine, being immersed in 1000 times of dilute solutions of 40% polyaminopropyl biguanide 1min is handled, fresh fruit remained on surface dirt, surface pathogenic microorganisms is removed, acts on the latent pathogenic bacteria in part, drain standby With;
(3) biological preservation is handled: by the granulated sugar tangerine after disinfection treatment, being immersed in 15% bacillus subtilis R31 biology guarantor Fresh dose of 300 times of dilute solutions handle 2min, the vacant room after disinfection is placed in after processing, windowing ventilation;
(4) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(5) result: it is shown in Table 1.
The formula (weight ratio) of the 40% polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, Excess water.
The citrus bio-preservative of the 15% bacillus subtilis R31 is every gram containing 3 × 1010The withered grass gemma of cfu The microbial bacterial agent product of bacillus R31 gemma.
The composition and proportion of the citrus bio-preservative of 15% bacillus subtilis R31 of the present invention are as follows:
(1) 15 grams of bacillus subtilis R31 original powder is weighed, 30 grams of chitosan oligosaccharide, 20 grams of rapeseed oil, Brij 9 Gram, 10 grams of Tween-80 (Tween 80) is mixed 2 hours in mixing kettle;
(2) 1.5 grams of potassium sorbate of the addition into the mixing kettle after step (1) stirring 2 hours, 2 grams of sodium acetate, 1 gram of sucrose, And rapeseed oil is supplied to 100%, continue stirring 0.5 hour, the oil of the stable living spores of R31 containing bacillus subtilis can be formed Suspending agent.
Reference examples 10
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) cleaning and sterilizing is handled: by stand-by granulated sugar tangerine, being immersed in 1000 times of dilute solutions of 40% polyaminopropyl biguanide 1min is handled, fresh fruit remained on surface dirt, surface pathogenic microorganisms is removed, acts on the latent pathogenic bacteria in part, drain, lead to Wind;
(3) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(4) result: it is shown in Table 1.
The formula (weight ratio) of the 40% polyaminopropyl biguanide aqua: polyaminopropyl biguanide 40%, ethylene glycol 4%, Excess water.
Reference examples 11
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten granulated sugar tangerine as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) biological preservation is handled: by stand-by granulated sugar tangerine, being immersed in 300 times of 15% bacillus subtilis R31 antistaling agent Dilute solution handles 2min, the vacant room after disinfection is placed in after processing, windowing ventilation;
(3) packing processes: choosing machinery wound rotten fruit after 5 days, and with the Polythene Bag individual packing with a thickness of 0.03mm, Dress basket is placed in 20-25 DEG C, stores under the conditions of relative humidity (RH) 75-90%, observation morbidity Decay, 180 fruits of every processing It is real.Disease incidence/%=(hair the number of sick fruits/inspection the total number of fruits) × 100, commodity rate/%=[(check the total number of fruits-hair the number of sick fruits- The dogberries such as physiological disease)/check the total number of fruits] × 100
(4) result: it is shown in Table 1.
The citrus bio-preservative of the 15% bacillus subtilis R31 is every gram containing 3 × 1010The withered grass gemma of cfu The microbial bacterial agent product of bacillus R31 gemma.
The composition and proportion of the citrus bio-preservative of 15% bacillus subtilis R31 of the present invention are as follows:
(1) 15 grams of bacillus subtilis R31 original powder is weighed, 30 grams of chitosan oligosaccharide, 20 grams of rapeseed oil, Brij 9 Gram, 10 grams of Tween-80 (Tween 80) is mixed 2 hours in mixing kettle;
(2) 1.5 grams of potassium sorbate of the addition into the mixing kettle after step (1) stirring 2 hours, 2 grams of sodium acetate, 1 gram of sucrose, And rapeseed oil is supplied to 100%, continue stirring 0.5 hour, the oil of the stable living spores of R31 containing bacillus subtilis can be formed Suspending agent.
Influence of the different embodiments to sugar orange rotting rate and commodity rate see the table below 1.
Influence of 1 Preservation Treatment of table to sugar orange rotting rate and commodity rate
20-25 DEG C of room temperature, store 30 days under the conditions of relative humidity (RH) 75-90%, the sugar orange of each processing group rot and Commodity rate situation is as described in Table 1.As can be known from the results: citrus green ecological preservation method of the invention can effectively reduce sugar orange Rotting rate improves commodity rate, with the effect of chemical bactericide Prochloraz (reference examples 1) quite even preferably effect;But it is a kind of Citrus green ecological preservation method, disinfection treatment are complementary organic wholes with biological preservation processing, are only used simultaneously In the case of, it can just guarantee individually to make with effect similar in chemical bactericide regardless of disinfection treatment or biological preservation are handled With the commodity rate that cannot be all effectively ensured in sugar orange storage period.

Claims (2)

1. a kind of green ecological preservation method of citrus, its step are as follows:
(1) fruit is used in screening storage: selecting to use without mechanical wounding, no disease and pests harm, without rotten citrus fruit as fresh-retaining preserving Fruit, cleaning are stand-by;
(2) it disinfects: citrus fruit stand-by obtained by step (1) is immersed at polyaminopropyl biguanide disinfection listerine Reason 1-2 minutes, to remove fresh citrus fruit remained on surface dirt, surface pathogenic microorganisms, act on the latent pathogenic bacteria in part, It is drained and standby;
(3) biological preservation is handled: by the citrus fruit after step (2) disinfection treatment, being immersed in the life of the R31 containing bacillus subtilis Object fresh-keeping liquid is handled 1-2 minutes, and processing is placed in basket, is placed in disinfection storehouse ventilation airing;
(4) packing processes: choosing machinery wound rotten fruit after step (3) treated citrus fruit is placed 3-5 days, and with poly- second Alkene bag individual packing is stored after filling basket in suitable condition;It is logical that polyaminopropyl biguanide described in the step (2) sterilizes listerine The polyaminopropyl biguanide aqua product for crossing 40 weight % is diluted by 500 times~1000 multiples;The 40 weight %'s is poly- The formula of aminopropyl biguanides aqua are as follows: polyaminopropyl biguanide 40%, ethylene glycol 4%, excess water.
2. the green ecological preservation method of citrus according to claim 1, it is characterised in that: described in the step (3) The biological preservation liquid of the R31 containing bacillus subtilis by the R31 containing bacillus subtilis citrus bio-preservative, by 200 times~800 Times multiple dilutes, and the citrus bio-preservative of the R31 containing bacillus subtilis is wettable powder or oil-suspending agent.
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