CN101589678A - The hypsizygus marmoreus produced by applying liquid strains original hase elicits method - Google Patents
The hypsizygus marmoreus produced by applying liquid strains original hase elicits method Download PDFInfo
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- CN101589678A CN101589678A CNA2009100575281A CN200910057528A CN101589678A CN 101589678 A CN101589678 A CN 101589678A CN A2009100575281 A CNA2009100575281 A CN A2009100575281A CN 200910057528 A CN200910057528 A CN 200910057528A CN 101589678 A CN101589678 A CN 101589678A
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Abstract
The present invention elicits method for a kind of hypsizygus marmoreus produced by applying liquid strains original hase, may further comprise the steps: 1) Hypsizygus marmoreus reaches the physical and chemical index parameter and the cultivation cycle of physiological ripening; 2) carry out low temperature stimulation before the optional step 1 described Hypsizygus marmoreus culture bottle that reaches physiological ripening, mycelium stimulation or behind the mycelium stimulation, the fertility chamber is put in mycelium stimulation, water filling into; 3) will recover the environmental management in stage according to mycelia through the culture bottle of step 2 processing; 4) annesl and original hase form the environmental management in stage; 5) the sprout environmental management in stage.The adjusting of the logical envirment factor of state of the present invention has promoted the formation of culture bottle charge level original hase effectively, broken the convention of the old bacterium piece of traditional main dependence fruiting, solved the problem of using liquid spawn fruiting difficulty, for the application of liquid spawn in hypsizigus marmoreus in factory production provides solid technical foundation.
Description
Technical field
The present invention relates to a kind of hypsizigus marmoreus in factory production new technology, particularly disclose a kind of hypsizygus marmoreus produced by applying liquid strains original hase and elicit method, be to use liquid spawn to carry out one of hypsizigus marmoreus in factory production key technology.
Background technology
Solid spawn is used in conventional hypsizigus marmoreus in factory production, relies on old bacterium piece fruiting, original hase (the kink body that the mycelia before the edible mushroom forms, turn to the dress of reproduction to indicate by nourishing and growing) on old bacterial classification, form, the original hase differentiation condition is relatively easy, and the technology of comparative maturity is arranged.But be to use after the liquid spawn, do not have old bacterium piece, original hase need directly form on the cultivate material surface, adopts conventional inductive technology, and original hase forms difficulty.
Summary of the invention
The objective of the invention is adjusting, directly induce cultivate material surface original hase to form, solve the problem of hypsizigus marmoreus in factory production application liquid spawn fruiting difficulty by envirment factor.
The present invention is achieved in that a kind of hypsizygus marmoreus produced by applying liquid strains original hase elicits method, may further comprise the steps:
1, Hypsizygus marmoreus reaches the physical and chemical index parameter and the cultivation cycle of physiological ripening:
Culture bottle bottle wall color yellow fraction, the dense yellow fraction of composts or fertilisers of cultivating surface aerial hyphae, the composts or fertilisers of cultivating color is yellowish, quality is soft, dense mycelia fragrance is arranged, and the pH value is 5.2～5.7, and water content is 72～74%, and cultivation cycle is 50～80 days;
2, carry out 12～24 hours, 5～10 ℃ low temperature stimulations before the optional step 1 described Hypsizygus marmoreus culture bottle that reaches physiological ripening, mycelium stimulation or behind the mycelium stimulation, expect that warm fall is more than 10 ℃, scratch the mycoderma on surface, water filling 10～15ml puts the fertility chamber into;
3, will according to following way to manage, impel mycelia to recover through the culture bottle of step 2 processing:
The 1st～5 day: 16～17 ℃ of temperature, air humidity 98～100%, gas concentration lwevel is less than 2000ppm, dark culturing;
Through after the processing in above-mentioned stage, the charge level mycelia recovers pure white but does not have aerial hyphae;
4, annesl and original hase form the environmental management in stage:
The 6th～9 day: 15～16 ℃ of temperature, air humidity 95～98%, gas concentration lwevel is less than 1500ppm,
Illumination is used: open 12～10 hours, 6～10 hours illumination in pass and dark alternately management.
Intensity of illumination: 500～1000lx,
Illumination type: fluorescent lamp;
Through after the above-mentioned processing, the charge level color becomes grey, and the mycelia kink forms the tiny globule of original hase justacrine;
5, the sprout environmental management in stage:
The 10th～13 day: 13～15 ℃ of temperature, air humidity 90～95%, gas concentration lwevel is less than 2000ppm,
Illumination is used: open 6～10 hours, 18～14 hours illumination in pass and dark alternately management,
Intensity of illumination: 500～1000lx,
Illumination type: fluorescent lamp;
Through after the above-mentioned processing, culture bottle charge level small mushroom bud forms.After this regulate the envirment factor of fertility chamber according to normal management of producing mushroom.
The invention has the beneficial effects as follows: adopt low temperature stimulation to promote that mycelia transforms to reproductive growth by nourishing and growing, promote mycelia to recover by dark culturing, high light promotes charge level annesl and original hase to form with dark alternately the stimulation, the temperature that suits, humidity and ozone, make the cultivation charge level finish annesl in 8～10 days and form original hase, charge level formed small mushroom bud in 12～13 days.The present invention has promoted the formation of culture bottle charge level original hase effectively by the adjusting of envirment factor, broken the convention of the old bacterium piece of traditional main dependence fruiting, solved the problem of using liquid spawn fruiting difficulty, for the application of liquid spawn in hypsizigus marmoreus in factory production provides reliable guarantee.
Embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment 1:
1, Hypsizygus marmoreus reaches the physical and chemical index parameter and the cultivation cycle of physiological ripening:
Culture bottle bottle wall color yellow fraction, the dense yellow fraction of composts or fertilisers of cultivating surface aerial hyphae, the composts or fertilisers of cultivating color is yellowish, quality is soft, dense mycelia fragrance is arranged, and the pH value is 5.2～5.7, and water content is 72～74%.Cultivation cycle is 65 days (a 850ml culture bottle).
2, the optional step 1 described Hypsizygus marmoreus culture bottle that reaches physiological ripening, after placing 12～24 hours under 5～10 ℃ the environment, the material temperature descends and surpasses 10 ℃, scratches the mycoderma on surface, water filling 10～15ml.(mycelium stimulation behind the first low temperature stimulation)
3, will according to following way to manage, impel mycelia to recover through the culture bottle of step 2 processing:
The 1st～5 day: 16～17 ℃ of temperature, air humidity 98～100%, gas concentration lwevel is less than 2000ppm, dark culturing.
Through after the processing in above-mentioned stage, the charge level mycelia recovers pure white but does not have aerial hyphae.
4, annesl and original hase form the environmental management in stage:
The 6th～9 day: 15～16 ℃ of temperature, air humidity 95～98%, gas concentration lwevel is less than 1500ppm.
Illumination is used: open 12～10 hours, 6～10 hours illumination in pass and dark alternately management.
Intensity of illumination: 500～1000lx.
Illumination type: fluorescent lamp.
Through after the above-mentioned processing, the charge level color becomes grey, and the mycelia kink forms the tiny globule of original hase justacrine.
5, the sprout environmental management in stage:
The 10th～13 day: 13～15 ℃ of temperature, air humidity 90～95%, gas concentration lwevel is less than 2000ppm.
Illumination is used: open 6～10 hours, 18～14 hours illumination in pass and dark alternately management.
Intensity of illumination: 500～1000lx.
Illumination type: fluorescent lamp.
Through after the above-mentioned processing, culture bottle charge level small mushroom bud forms.After this according to normal fruiting.
Embodiment 2:
1, Hypsizygus marmoreus reaches the physical and chemical index parameter and the cultivation cycle of physiological ripening:
Culture bottle bottle wall color yellow fraction, the dense yellow fraction of composts or fertilisers of cultivating surface aerial hyphae, the composts or fertilisers of cultivating color is yellowish, quality is soft, dense mycelia fragrance is arranged, and the pH value is 5.2～5.7, and water content is 72～74%.Cultivation cycle is 70 days (a 1100ml culture bottle).
2, select the 1 described Hypsizygus marmoreus culture bottle that reaches physiological ripening for use, scratch the mycoderma on surface, water filling 10～15ml puts the fertility chamber into, within 12～24 hours temperature is being controlled at 5～10 ℃ (low temperature stimulations behind the first mycelium stimulation);
3, will be through 2 culture bottles of handling, composts or fertilisers of cultivating material temperature descends before than mycelium stimulation and surpasses more than 10 ℃, according to following way to manage, impels mycelia to recover:
The 1st～5 day: 16～17 ℃ of temperature, air humidity 98～100%, gas concentration lwevel is less than 2000ppm, dark culturing.
Through after the processing in above-mentioned stage, the charge level mycelia recovers pure white but does not have aerial hyphae.
4, annesl and original hase form the environmental management in stage:
The 6th～9 day: 15～16 ℃ of temperature, air humidity 95～98%, gas concentration lwevel is less than 1500ppm.
Illumination is used: open 12～10 hours, 6～10 hours illumination in pass and dark alternately management.
Intensity of illumination: 500～1000lx.
Illumination type: fluorescent lamp.
Through after the above-mentioned processing, the charge level color becomes grey, and the mycelia kink forms the tiny globule of original hase justacrine.
5, the sprout environmental management in stage:
The 10th～13 day: 13～15 ℃ of temperature, air humidity 90～95%, gas concentration lwevel is less than 2000ppm.
Illumination is used: open 6～10 hours, 18～14 hours illumination in pass and dark alternately management.
Intensity of illumination: 500～1000lx.
Illumination type: fluorescent lamp.
Through after the above-mentioned processing, culture bottle charge level small mushroom bud forms.After this regulate the envirment factor of fertility chamber according to normal management of producing mushroom.
Claims (1)
1. a hypsizygus marmoreus produced by applying liquid strains original hase elicits method, may further comprise the steps:
1) Hypsizygus marmoreus reaches the physical and chemical index parameter and the cultivation cycle of physiological ripening:
Culture bottle bottle wall color yellow fraction, the dense yellow fraction of composts or fertilisers of cultivating surface aerial hyphae, the composts or fertilisers of cultivating color is yellowish, quality is soft, dense mycelia fragrance is arranged, and the pH value is 5.2～5.7, and water content is 72～74%, and cultivation cycle is 50～80 days;
2) carry out 12～24 hours, 5～10 ℃ low temperature stimulations before the optional step 1 described Hypsizygus marmoreus culture bottle that reaches physiological ripening, mycelium stimulation or behind the mycelium stimulation, expect that warm fall is more than 10 ℃, scratch the mycoderma on surface, water filling 10～15ml puts the fertility chamber into;
3) will according to following way to manage, impel mycelia to recover through the culture bottle of step 2 processing:
The 1st～5 day: 16～17 ℃ of temperature, air humidity 98～100%, gas concentration lwevel is less than 2000ppm, dark culturing;
Through after the processing in above-mentioned stage, the charge level mycelia recovers pure white but does not have aerial hyphae;
4) annesl and original hase form the environmental management in stage:
The 6th～9 day: 15～16 ℃ of temperature, air humidity 95～98%, gas concentration lwevel is less than 1500ppm,
Illumination is used: open 12～10 hours, 6～10 hours illumination in pass and dark alternately management,
Intensity of illumination: 500～1000lx,
Illumination type: fluorescent lamp;
Through after the above-mentioned processing, the charge level color becomes grey, and the mycelia kink forms the tiny globule of original hase justacrine;
5) the sprout environmental management in stage:
The 10th～13 day: 13～15 ℃ of temperature, air humidity 90～95%, gas concentration lwevel is less than 2000ppm,
Illumination is used: open 6～10 hours, 18～14 hours illumination in pass and dark alternately management,
Intensity of illumination: 500～1000lx,
Illumination type: fluorescent lamp;
Through after the above-mentioned processing, culture bottle charge level small mushroom bud forms, and after this regulates the envirment factor of fertility chamber according to normal management of producing mushroom.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2009100575281A CN101589678B (en) | 2009-07-01 | 2009-07-01 | Method for inducing primordium of hypsizygus marmoreus produced by applying liquid strains |
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| CN2009100575281A CN101589678B (en) | 2009-07-01 | 2009-07-01 | Method for inducing primordium of hypsizygus marmoreus produced by applying liquid strains |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104316478A (en) * | 2014-10-28 | 2015-01-28 | 福建农林大学 | Determination method of hypsizigus marmoreus fungi pack ripeness |
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| CN100431402C (en) * | 2005-09-29 | 2008-11-12 | 上海丰科生物科技股份有限公司 | Cultivation of brown crab-flavor mushroom |
| CN101293791A (en) * | 2008-06-13 | 2008-10-29 | 上海浦东天厨菇业有限公司 | Liquid bacterial culture medium for industrial preparation of hypsizygus marmoreus and preparation method thereof |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104316478A (en) * | 2014-10-28 | 2015-01-28 | 福建农林大学 | Determination method of hypsizigus marmoreus fungi pack ripeness |
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