CN101584585A - Experimental system for the determination of white rat behavior - Google Patents
Experimental system for the determination of white rat behavior Download PDFInfo
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Abstract
The invention discloses an experimental system for the determination of white rat behavior, which is composed of two parts including a hardware device and a white rat video tracking determination system consisting of system hardware and system software, the hardware device comprises a box body, an animal movement case arranged within the box body, an illumination device arranged on the top of the box body and an outdoor air system connected with the box body; the system hardware comprises a video input unit, an image acquisition card and a computer, wherein, the video input unit is connected with the computer via a video line and is arranged on the top of the box body, the image acquisition card and the system software are installed on the computer, the system software comprises the following modules: image acquisition, trace tracking, background modeling, computation of characteristic data, and formatted display and storage of result. The experimental system implements the acquisition of behavior data of the white rat on conditions of low noise and low illumination, causing no disturbance for normal activity of the white rat and improving accuracy of experimental result; the invention, in combination with the white rat video tracking determination system, performs the determination flexibly and results from diversified test parameters.
Description
Technical field
The present invention relates to animal behavior determination experiment system, be specifically related to a kind of white mouse behavior determination experimental system.
Background technology
Drug dependence is a kind of chronic recurrent encephalopathy, mainly shows as the uncontrollability that forces drug taking behavior and dosage to addictive drug.Cause the reason of addiction to comprise positive reinforcement factor (as sense of euphoria, rewarding effect etc.), negative reinforcement factor (as trying to escape reality, alleviate withdrawal symptom etc.) and conditional reinforcement factor.Research at present thinks that rewarding effect that addictive drug brings is to bring out drug dependence, cause mandatory drug-seeking behavior and one of the main reason of reverting to take drugs.Be anthropomorphic dummy's drug-seeking behavior from different perspectives, the positive reinforcement effect of reaction medicine, the animal behavior pharmacology model of at present existing multiple evaluation drug dependence, develop the comparatively sophisticated self administration (self-administration that mainly comprises, SA), drug discrimination (drug discrimination, DD), (locomotor activity LA) measures for conditioned place preference (conditioned place preference is called for short CPP) experiment and locomotor activity.Therefore, can carry out the foundation of the experimental system of behavior determination to white mouse automatically, research has extremely important assosting effect to drug dependence.
Yet at present the white mouse behavior determination experimental system of domestic and international application generally adopts the photocell robot scaler, and the information index that exists experiment to obtain is single, can not observe defective such as white mouse activity situation in the experimentation.
Along with the development of digital media technology and computer vision technique, make that adopting computer vision technique that the animal behavior in the video input unit visual field is discerned automatically with mensuration becomes possibility.But how to make up and realize high accuracy, high automation, Computationally efficient and can satisfy the white mouse behavior determination system of white mouse biotic experiment environment specific (special) requirements, be current technological difficulties and design difficulty always.For example, how to eliminate the influence to behavior determination of urine that the white mouse activity stays, how the position when how accurately to follow the tracks of the different behavior state of white mouse keeps the low noise of experimental situation and low-light (level) etc.
Summary of the invention
The invention provides a kind of white mouse behavior determination experimental system, adopt hardware unit and software two parts to combine, can under low noise, low light conditions, carry out the conditioned place preference experiment, the experimental period of this experimental system is controlled, even have urine also can accurately obtain multinomial white mouse characteristic, and complete monitoring white mouse movement track.
A kind of white mouse behavior determination experimental system, mainly measure system's two parts by hardware unit and white mouse video tracking and form, described hardware unit comprises casing, places the intravital animal activity box of case, places the illuminator at casing top and the VMC (Ventilation Mechanical Control System) that is connected with casing; Described white mouse video tracking is measured system and is made up of system hardware and systems soft ware, system hardware comprises video input unit, image pick-up card and computer, video input unit connects computer and places the casing top by video line, image pick-up card, systems soft ware are installed on computers, and systems soft ware comprises following module: image acquisition, parameter setting, trajectory track, background modeling, characteristic are calculated, the result formats and shows and preservation.
Described casing is the sound insulation casing, can adopt the thick baffle of 1～4mm to prepare the sound insulation casing, such as a kind of model by the butterfly deadener company limited production of Shenzhen China is the efficient sound insulating sheet material of macromolecule of ep100, it has efficient sound insulation value, water-fast, ageing-resistant, weather-proof, anti-ozone, 2 grades of the awkward combustions of fire prevention level.
Described VMC (Ventilation Mechanical Control System) can be selected the general existing product in this area for use, and the preferred background noise can be controlled in 30 decibels (dB) following VMC (Ventilation Mechanical Control System), so that for animal provides the normal environment of an activation, avoid the interference of noise.
Described illuminator can be existing array LED (LED) or other illuminator that is used to throw light on; Array LED can be evenly distributed on around the video input unit during as illuminator, brightness by controlled array LED control animal activity box, can be experimental actual needs regulate average illumination＜20 luxs (Lux) of the animal activity box that places box bottom, for animal provides the normal environment of an activation.
Described animal activity box places in the image acquisition scope of video input unit and get final product, be preferably placed at video input unit under, be convenient to gather the Sports Series image of interior each animal of animal activity box.
Described animal activity box is made up of several boxes that shuttle back and forth, be provided with an active dividing plate in the middle of each box that shuttles back and forth, this dividing plate training stage is the enclosed type dividing plate, test phase is the channel-style dividing plate, the box that will shuttle back and forth is divided into first box and second box, and wherein around first box and bottom surface is white, and the bottom surface is smooth, around second box and bottom surface is black, and grid is engraved in the bottom surface.
The described box that shuttles back and forth can select for use polrvinyl chloride (PVC) material to prepare.
Described video input unit, image pick-up card, computer and video input line all can adopt the standardization device of directly purchasing.
Described white mouse video tracking is measured the systems soft ware of system for to measure software automatically towards behavioristics's video of white mouse, is applicable to the mensuration of the white mouse behavior video of dedicated experiments case.
The animal movement image series that the white mouse behavior determination experimental stage gathers can adopt white mouse video tracking mensuration system to measure the indexs such as position preference, locomotor activity and exploratory behaviour of being tried white mouse, and these indexs can show by the characteristic of being tried white mouse.
The step that white mouse video tracking mensuration system carries out white mouse behavior videometry is as follows:
(I) system hardware that the white mouse video tracking is measured system is installed;
(II) systems soft ware that the white mouse video tracking is measured system is installed, the program of desire being carried out white mouse behavior videometry systems soft ware deposits in the program storage of computer;
(III) start the working procedure that the white mouse video tracking is measured system, the initialization of the use video input unit that this working procedure provides according to equipment producer, image pick-up card, computer requires to set initialization values, working procedure carries out initialization to the system hardware of white mouse video tracking mensuration system respectively according to these initialization values, comprises the initialization of video input unit, image pick-up card, computer; The videometry visual field that the white mouse video tracking is measured system is set in being initialized as of video input unit, sets the sampling period that the white mouse video tracking is measured system;
Described white mouse video tracking is measured the zone of the videometry visual field of system for several box places of shuttling back and forth;
(IV) the white mouse video tracking video input unit of measuring system was gathered the Sports Series image that the animal in the videometry visual field produces in chronological order and is write down corresponding acquisition time according to the predefined sampling period, and video input unit is input to image pick-up card with the Sports Series view data that collects;
(V) image pick-up card carries out analog digital conversion in chronological order to the Sports Series view data, makes video signal become Digital Image Data, and Digital Image Data is transferred to computer;
The mode that white mouse video tracking mensuration system carries out white mouse behavior videometry comprises: the black area background modeling is measured mode and not modeling mensuration mode, carry out before the white mouse behavior videometry Sports Series view data that collects being estimated, when on estimating the Sports Series view data, urine being arranged, the artificial appointment adopted black area background modeling mensuration mode, this moment background preserve to take place and only occur in follow the trail of white mouse as a result the position in the white area, background is preserved back execution in step (VI); When on estimating the Sports Series view data, not having urine, artificial appointment is adopted the black area background modeling is measured mode or adopted not modeling to measure mode, background was preserved back execution in step (VI) when employing was measured mode to the black area background modeling, direct execution in step (VI) when adopting not modeling to measure mode;
(VI) tracing point of animal position in each frame of digital image calculates in white mouse video tracking mensuration system by the white mouse track algorithm;
(VII) the white mouse video tracking is measured the tracing point connection of system with white mouse position in each frame of digital image, generates the movement track figure of white mouse;
(VIII) experimental actual needs, the working procedure of white mouse video tracking mensuration system can calculate the characteristic of white mouse according to the movement track figure of white mouse, and the every trade of going forward side by side is for measuring;
(IX) computer shows movement sequence image, movement track figure and the characteristic of white mouse and be stored in the Computer Database, is convenient to consult at any time.
When the systems soft ware that described white mouse video tracking is measured system specifically uses, need be provided with in advance, comprise: design white mouse behavior videometry systems soft ware outward appearance, create white mouse behavior video videometry systems soft ware class, definition of user's interface etc.
After the setting that described white mouse video tracking is measured the systems soft ware of system is finished, adopt computer that Digital Image Data is measured, this determination step is as follows:
Set and measure the zone, measure the zone and be the zone corresponding on the Digital Image Data with the box that shuttles back and forth;
It is newly-built that the zone is carried out in the mensuration zone of opening setting, the zone is divided into two sub-districts along the drop front upper limb with each tracing area (on the tracing area Digital Image Data with a corresponding zone of the box that shuttles back and forth) when newly-built: white area and black area, the white area is zone corresponding with first box on the Digital Image Data, and the black area is zone corresponding with second box on the Digital Image Data; Each sub-district delimited and be central circular and two parts zone, neighboring area;
Carry out the parameter setting, comprising: it is a certain tracing area that the zoning is set, the setting area width, and its width with the box that shuttles back and forth is consistent and gets final product; Respectively corresponding threshold of black area and white area is set, systems soft ware according to the black area threshold value that is provided with and white area threshold value with each frame of digital image binaryzation; The area ratio of central circular area and the sub-district gross area is set, can selects according to actual needs this area ratio is set at 1/2,1/4 or 1/8; Concrete start-stop frame number is set, and the start-stop frame number that can calculate is as required selected.
According to black area threshold value that sets and white area threshold value, black area and white area binaryzation with each frame of digital image, some black connected regions (two kinds of pixels of only remaining black and white after the binaryzation) in labelling black area and the white area then, in some black connected regions of above-mentioned labelling, find the black connected region of area maximum again, and get center of gravity and be the tracing point of white mouse at this frame of digital image.
The sub-district at white mouse tracing point place in each frame of digital image of location determination: successively adjacent tracing point is two sub-districts, judges that then white mouse shuttles back and forth once at the box that shuttles back and forth, and accumulative total obtains the shuttle back and forth number of times of white mouse at this box that shuttles back and forth; To the digital picture of successively adjacent tracing point in same sub-district, add up time and distance between two successively adjacent tracing points of two sub-districts respectively, obtain white mouse in the total residence time of each sub-district and the distance of pacing up and down.
Described characteristic is the operating range of white mouse, white mouse is in time that first box (being white box) always stays, white mouse is in time that second box (being black box) always stays, the time that white mouse stays in each central area, the shuttle back and forth number of times of white mouse in the box that shuttles back and forth, the distances of pacing up and down of white mouse etc. characterize the characteristic of white mouse behavior, to measure the behavior of white mouse.
Through judging, specify and adopt not modeling to measure mode, directly during execution in step (VI), the white mouse track algorithm of execution is as follows:
To each frame of digital image:
A. select tracing area;
B. according to using black area threshold value and white area threshold value always with black area in the tracing area and white area binaryzation;
Definite process of black area threshold value commonly used and white area threshold value is as follows:
By manually rule of thumb determining black area initial threshold and white area initial threshold, the systems soft ware that the white mouse video tracking is measured system carries out binaryzation according to black area initial threshold and white area initial threshold to each frame of digital image in advance;
During binaryzation, extract the blue component and the comparison of white area initial threshold of Pixel of Digital Image color in the white area, if blue component than white area initial threshold greatly then two-value turns to black, if blue component is littler then two-value turns to white than white area initial threshold;
0.1 red component that extracts the Pixel of Digital Image color in the black area adds 0.9 green component, compare with the black area initial threshold, if it is 0.1 red component adds 0.9 green component than black area initial threshold greatly then two-value turns to white, littler then two-value turns to black than black area initial threshold if 0.1 red component adds 0.9 green component;
Behind each frame of digital pictorial display binaryzation result, if the corresponding black picture element of white mouse do not occur, need reduce the white area threshold value or increase the black area threshold value or increase black area threshold value when reducing the white area threshold value, until showing the black picture element corresponding with the white mouse position, to track white mouse, this moment, threshold value was white area optimal threshold and black area optimal threshold, and the white area optimal threshold is defined as the white area threshold value, and the black area optimal threshold is defined as black area commonly used threshold value;
C. adopt the black connected region in (algorithm that Rosenfeld and Pfaltz proposed in 1966) labelling black area of labeling algorithm line by line and white area;
D. find out the maximum black connected region in black area and the white area, and calculate center of gravity, center of gravity is the tracing point of white mouse position;
Through judging, specify to adopt the black area background modeling is measured mode, when background was preserved back execution in step (VI), with the position that accurate tracking is tried white mouse, the white mouse track algorithm of execution was as follows:
To each frame of digital image:
A. select tracing area;
Tried white mouse and when the white area, the black area background is preserved automatically if track in this frame of digital image, when handling the black area of next frame digital picture, the pixel value of being had a few on this frame of digital picture black is deducted the pixel value of corresponding point on the previous frame digital picture black area, obtain the new digital picture black area of this frame;
B. according to background modeling black area threshold value and white area threshold value with black area in the tracing area and white area binaryzation;
Definite process of background modeling black area threshold value and white area threshold value is as follows:
Background modeling black area threshold value can set up on their own, and setting background modeling black area threshold value generally speaking is 5, also can adjust according to actual needs.0.1 red component that in the new digital picture black area of every frame, extracts the Pixel of Digital Image color add 0.9 green component as the RGB component and with 5 relatively, when the RGB component and greater than 5 the time two-value turn to white pixel, when the RGB component be black picture element less than 5 the time.
Mode is measured in this moment white area threshold value and described not modeling of employing, and directly the white area threshold value in the white mouse track algorithm of execution is identical during execution in step (VI).
C. adopt the black connected region of labeling algorithm labelling black area and white area line by line;
D. find out the maximum black connected region in black area and the white area, and calculate center of gravity, center of gravity is the tracing point of white mouse position;
Described video white mouse track algorithm adopts false code to realize.
Described tracing area be on the Digital Image Data with a zone that the box that shuttles back and forth is corresponding.
The present invention has following advantage:
(1) being captured under low noise, the low light conditions of white mouse behavioral data carried out, and do not disturb the normal activity of white mouse, improves the accuracy of experimental result;
(2) hardware unit adopts the standardization industrial design, and is sturdy and durable;
(3) adopt white mouse video tracking technology, the automatization that has realized experimentation has avoided the subjective error of artificial counting introducing and to testing the interference of white mouse, has increased the true and reliable property of experimental result;
(4) movement sequence image and the measurement result of white mouse are noted and are kept in the Computer Database, the experimental data data is true, reliable, but the inspection of artificial school is carried out in playback;
(5) Ling Xian core algorithm is the white mouse track algorithm, effectively guarantees the anti-interference and the accuracy of identification;
(6) adopt open, modularized design, the system expandability is strong, can be external other mensuration module, the data and the index result that calculate according to the movement track figure of white mouse can import to Excel, be convenient to the user and do further to measure processing in statistical software;
(7) the mensuration mode is flexible, supports the period to measure, and supports regularly to stop and artificial the termination, and has an abundant display mode, motion conditions to white mouse can adopt multiple display mode performance such as trajectory diagram, parameter index, curve, rectangular histogram, and can generate complete report, for printout;
(8) this systems soft ware has been realized white mouse physiology behavior characteristics data computing, comprise calculating to residence time, the distance of pacing up and down and the number of times that shuttles back and forth, also can show the tracing point that is tried the every frame position of white mouse, its algorithm is simply effective, finding speed is fast, and can record simultaneously and measure many white mouse in the black area, the time of staying, the activity distance in white area, a plurality of characteristics such as number of times and movement locus of shuttling back and forth.
(9) adopt the experiment hardware condition to distinguish background down and (add 0.9 green component as adopting 0.1 red component in the black area with best RGB component that is tried white mouse and weight, second threshold setting is 5 etc.), in conjunction with existing labeling algorithm line by line, accurately to follow the trail of and tried white mouse, algorithm is simple fast effectively.
Description of drawings
Fig. 1 is the structural representation of white mouse behavior determination experimental system of the present invention;
Fig. 2 is the structural representation of animal activity box in the experimental system of the present invention;
Fig. 3 carries out the FB(flow block) of white mouse behavior videometry for the present invention adopts white mouse video tracking mensuration system.
The specific embodiment
As shown in Figure 1 and Figure 2, white mouse behavior determination experimental system of the present invention, mainly measure system's two parts by hardware unit and white mouse video tracking and forms, hardware unit comprises casing 1, place the interior animal activity box 2 of casing 1, place the array LED 6 at casing 1 top and the FV-15PE2C of the Panasonic VMC (Ventilation Mechanical Control System) 5 that is connected with casing 1; The white mouse video tracking is measured system and is made up of system hardware and systems soft ware, system hardware comprises video input unit 3, image pick-up card and computer 4, video input unit 3 connects computer 4 and places casing 1 top by video line, image pick-up card, systems soft ware are installed on the computer 4, and systems soft ware comprises following module: image acquisition, parameter setting, trajectory track, background modeling, characteristic are calculated, the result formats and shows and preservation.
The sound insulation casing that the baffle that casing 1 is ep100 for model thick with 4mm, Shenzhen magnificent butterfly deadener company limited production is made, casing 1 specification is long 160cm, wide 110cm, high 110cm.
The FV-15PE2C of Panasonic VMC (Ventilation Mechanical Control System) 5 comprises that exhaust fan, main frame, ventilation shaft, exhaust outlet and window advance device, the background noise＜30dB of this VMC (Ventilation Mechanical Control System) 5.
Array LED 6 is evenly distributed on around the video input unit 3, by the brightness of controlled array LED control animal activity box 2, regulates the average illumination＜20Lux of the animal activity box 2 that places casing 1 bottom.
Animal activity box 2 be positioned at video input unit 3 under, its specification is long 45cm, wide 45cm, high 15cm, form by 4 polrvinyl chloride box 7 that shuttles back and forth, polrvinyl chloride shuttles back and forth the specification of box 7 for long 30cm, wide 15cm, high 15cm, each polrvinyl chloride shuttles back and forth has an active dividing plate in the middle of the box 7, this dividing plate training stage is the enclosed type dividing plate, and test phase is the channel-style dividing plate, and the polrvinyl chloride box 7 that shuttles back and forth is divided into first box 8 and second box 9, the specification of first box 8 and second box 9 is long 15cm, wide 15cm, high 15cm, wherein around first box 8 and bottom surface is white, and the bottom surface is smooth, around second box 9 and bottom surface is black, and grid is engraved in the bottom surface, and grid is the square of 0.5cm * 0.5cm.
Conditioned place preference with morphine induction is an example, carries out following test:
1, natural preference test
Adopt the channel-style dividing plate that first box 8 and second box 9 are separated during test, white mouse is not made the intersection that any processing is positioned over first box 8 and second box 9, be allowed to condition at free movable 15min in first box 8 and second box 9, gather white mouse Sports Series image by video input unit 3, so that measure its natural preference situation.Retest three times, each test interval 24h.
2, the foundation and the expression of the experiment of morphine induction white mouse behavior determination
Before the training beginning all white mouse are carried out natural preference test, step is with 1.According to natural preference result and pertinent literature (Pharmacol Biochem Behav, 1989,33 (4): 765-775; Pharmacol Biochem Behav, 1988,30 (4): 889-893), natural preference box is difficult for setting up CPP, so select white box as companion's medicine box.Totally 9 days experimental period of whole C PP, comprise following two stages:
The establishment stage of white mouse behavior determination experiment: training period adopts the enclosed type dividing plate that first box 8 in the animal activity box 2 and second box 9 are isolated fully, 16 white mouse are divided into normal saline matched group (NS) and morphine group (Mor) at random, each organized 8, and morphine group (Mor) white mouse carried out lumbar injection morphine hydrochloride (5mg/kg) back respectively and places first box 8 (being white box) training 50min at the 1st day, the 3rd day, the 5th day, the 7th day; Normal saline matched group (NS) white mouse carried out intraperitoneal injection of saline (0.5ml/kg) back respectively and places second box 9 (being black box) training 50min at the 2nd day, the 4th day, the 6th day, the 8th day.The matched group white mouse all gives normal saline at every turn, and other is handled with morphine group mice.
The expression phase of white mouse behavior determination experiment: tested the 9th day, adopt the channel-style dividing plate between first box 8 and second box 9, white mouse is not done any processing be positioned over two box intersections, be allowed to condition at free movable 15min in two boxes, gather white mouse Sports Series image by video input unit 3.
3, to measure the step that white mouse behavior video that system gathers video input unit 3 measures as follows for the white mouse video tracking:
After correct installation white mouse video tracking was measured the system hardware and systems soft ware of system, the program of desire being carried out white mouse behavior videometry systems soft ware deposited in the program storage of computer 4;
Start the white mouse video tracking and measure the working procedure of system, the initialization of the use video input unit 3 that this working procedure provides according to equipment producer, image pick-up card, computer 4 requires to set initialization values, working procedure carries out initialization to the system hardware of white mouse video tracking mensuration system respectively according to these initialization values, comprises the initialization of video input unit 3, image pick-up card, computer 4; The videometry visual field that the white mouse video tracking is measured system that is initialized as of video input unit 3 is set at the shuttle back and forth zone at box 7 places of 4 polrvinyl chloride, sets the sampling period that the white mouse video tracking is measured system simultaneously;
Form the waiting period that the sampling period being by sampling period and sampling when natural preference was tested, wherein sampling period is 15min, is 24h waiting period of sampling; The sampling period is 15min during the expression phase of white mouse behavior determination experiment;
Video input unit 3 according to predefined sampling period gather 4 polrvinyl chloride shuttle back and forth white mouse in the zone at box 7 places the Sports Series image and write down corresponding acquisition time, video input unit 3 is input to image pick-up card with the white mouse Sports Series view data that collects;
Image pick-up card carries out analog digital conversion to each frame white mouse Sports Series view data, becomes Digital Image Data, and Digital Image Data is transferred to computer 4;
Referring to Fig. 3, the systems soft ware of the white mouse video tracking being measured system is provided with, and comprising: design white mouse behavior videometry systems soft ware outward appearance, establishment white mouse behavior video videometry systems soft ware class, definition of user's interface etc.;
Adopt 4 pairs of Digital Image Data of computer to measure then, determination step is as follows:
Set to measure the zone: tracing area α, tracing area β, tracing area γ and tracing area δ, tracing area α, tracing area β, tracing area γ and tracing area δ are respectively on the Digital Image Data with box 7 that 4 polrvinyl chloride are shuttled back and forth and carry out α, β, γ, δ numbering in the direction of the clock and corresponding zone;
It is newly-built that the corresponding Digital Image Data in mensuration zone of opening and setting is carried out the zone, the zone will be divided into two sub-districts respectively with each polrvinyl chloride corresponding zone (tracing area α, tracing area β, tracing area γ and tracing area δ) of box 7 of shuttling back and forth on the Digital Image Data along the drop front upper limb when newly-built: white area and black area, the white area is zone corresponding with first box 8 on the Digital Image Data, the black area is zone corresponding with second box 9 on the Digital Image Data, and each sub-district delimited and is central circular and neighboring area two parts;
Carry out the parameter setting, comprising: it is tracing area α, tracing area β, tracing area γ or tracing area δ that the zoning is set, and the setting area width is 15cm (itself and a polrvinyl chloride shuttle back and forth the width of box 7 be consistent);
The central circular area that each sub-district is set is 1/8 of the sub-district gross area;
Concrete start-stop frame number is set: as the 0th frame to the 22500 frames;
Carry out before the white mouse behavior videometry Sports Series view data that collects being estimated, when on estimating the Sports Series view data, urine being arranged, the artificial appointment adopted black area background modeling mensuration mode, this moment background preserve to take place and only occur in follow the trail of white mouse as a result the position in the white area, background is preserved back white mouse video tracking and is measured system and calculate the tracing point of white mouse position in each frame of digital image by the white mouse track algorithm that adopts false code to realize;
When on estimating the Sports Series view data, not having urine, artificial appointment is adopted the black area background modeling is measured mode or adopted not modeling mensuration mode, and background is preserved back white mouse video tracking mensuration system and calculated the tracing point of white mouse position in each frame of digital image by the white mouse track algorithm that adopts false code to realize when adopting black area background modeling mensuration mode; Directly carry out white mouse video tracking mensuration system when adopting not modeling to measure mode and calculate the tracing point of white mouse position in each frame of digital image by the white mouse track algorithm that adopts false code to realize;
Through judging, to specify when adopting not modeling to measure mode, the white mouse track algorithm of execution is as follows:
To each frame of digital image:
A. select tracing area α, tracing area β, tracing area γ and tracing area δ respectively;
B. according to the black area commonly used threshold value that is provided with and white area threshold value with black area in each tracing area and white area binaryzation;
Definite process of black area threshold value commonly used and white area threshold value is as follows:
By manually rule of thumb determining black area initial threshold and white area initial threshold, the systems soft ware that the white mouse video tracking is measured system carries out binaryzation according to black area initial threshold and white area initial threshold to each frame of digital image in advance;
During binaryzation, extract the blue component and the comparison of white area initial threshold of Pixel of Digital Image color in the white area, if blue component than white area initial threshold greatly then two-value turns to black, if blue component is littler then two-value turns to white than white area initial threshold;
0.1 red component that extracts the Pixel of Digital Image color in the black area adds 0.9 green component, compare with the black area initial threshold, if it is 0.1 red component adds 0.9 green component than black area initial threshold greatly then two-value turns to white, littler then two-value turns to black than black area initial threshold if 0.1 red component adds 0.9 green component;
Behind each frame of digital pictorial display binaryzation result, if the corresponding black picture element of white mouse do not occur, need reduce the white area threshold value or increase the black area threshold value or increase black area threshold value when reducing the white area threshold value, until showing the black picture element corresponding with the white mouse position, to track white mouse, this moment, threshold value was white area optimal threshold and black area optimal threshold, and the white area optimal threshold is defined as the white area threshold value, and the black area optimal threshold is defined as black area commonly used threshold value;
C. adopt Rosenfeld and Pfaltz in the labeling algorithm line by line that proposed in 1966 some black connected regions in black area and white area in the selected tracing area of labelling respectively, be some black connected regions in interior black area of tracing area α and white area, some black connected regions in black area and white area in the tracing area β, some black connected regions in interior black area of some black connected regions in black area and white area and tracing area δ and white area in the tracing area γ;
D. find out the maximum black connected region in interior black area of tracing area α, tracing area β, tracing area γ and tracing area δ and the white area respectively, and calculate center of gravity respectively, each center of gravity is the tracing point of white mouse in each tracing area position;
Through judging, to specify when adopting black area background modeling mensuration mode, the white mouse track algorithm of execution is as follows:
To each frame of digital image:
A. select tracing area α, tracing area β, tracing area γ and tracing area δ respectively;
Tried white mouse and when the white area, the black area background is preserved automatically if track in this frame of digital image, when handling the black area of next frame digital picture, the pixel value of being had a few on this frame of digital picture black is deducted the pixel value of corresponding point on the previous frame digital picture black area, obtain the new digital picture black area of this frame;
B. according to the background modeling black area threshold value that is provided with and white area threshold value with black area in each tracing area and white area binaryzation;
Definite process of background modeling black area threshold value and white area threshold value is as follows:
Background modeling black area threshold value can set up on their own, and setting background modeling black area threshold value generally speaking is 5, also can adjust according to actual needs.0.1 red component that in the new digital picture black area of every frame, extracts the Pixel of Digital Image color add 0.9 green component as the RGB component and with 5 relatively, when the RGB component and greater than 5 the time two-value turn to white pixel, when the RGB component be black picture element less than 5 the time.
White area threshold value in the white mouse track algorithm of carrying out when the white area threshold value is measured mode with described not modeling of employing this moment is identical.
C. adopt Rosenfeld and Pfaltz in the labeling algorithm line by line that proposed in 1966 some black connected regions in black area and white area in the selected tracing area of labelling respectively, be some black connected regions in interior black area of tracing area α and white area, some black connected regions in black area and white area in the tracing area β, some black connected regions in interior black area of some black connected regions in black area and white area and tracing area δ and white area in the tracing area γ;
D. find out the maximum black connected region in interior black area of tracing area α, tracing area β, tracing area γ and tracing area δ and the white area respectively, and calculate center of gravity respectively, each center of gravity is the tracing point of white mouse in each tracing area position;
The white mouse video tracking is measured the tracing point connection of system with white mouse position in each frame of digital image, generates the movement track figure of white mouse;
The working procedure of white mouse video tracking mensuration system calculates the characteristic of the white mouse in each tracing area according to the tracing point of white mouse in n frame to the n+m frame, characteristic comprises: the operating range of white mouse, white mouse is in time that first box 8 (being white box) always stays, white mouse is in time that second box 9 (being black box) always stays, the time that white mouse stays in each central area, the shuttle back and forth number of times of white mouse in the box 7 that shuttles back and forth, characteristics such as the distance of pacing up and down of white mouse obtain the characteristic measurement result; Wherein, n, m are positive integer;
Simultaneously the behavior of white mouse is measured the sub-district at white mouse tracing point place in each frame of digital image of location determination: successively adjacent tracing point is two sub-districts, judges that then white mouse shuttles back and forth once in polrvinyl chloride shuttles back and forth box 7, and accumulative total obtains the number of times that shuttles back and forth.To the digital picture of successively adjacent tracing point in same sub-district, add up time and distance between two successively adjacent tracing points of two sub-districts respectively, obtain white mouse in the total residence time of each sub-district and the distance of pacing up and down.
Computer 4 shows movement sequence image, movement track figure and the characteristic measurement result of white mouse and be stored among computer 4 data base on computer 4 display screens, is convenient to consult at any time.
Claims (9)
1, a kind of white mouse behavior determination experimental system, measure system's two parts by hardware unit and white mouse video tracking and form, it is characterized in that: described hardware unit comprises casing (1), places the interior animal activity box (2) of casing (1), places the illuminator at casing (1) top and the VMC (Ventilation Mechanical Control System) (5) that is communicated with casing (1); Described white mouse video tracking is measured system and is made up of system hardware and systems soft ware, system hardware comprises video input unit (3), image pick-up card and computer (4), video input unit (3) connects computer (4) and places casing (1) top by video line, image pick-up card, systems soft ware are installed on the computer (4), and systems soft ware comprises following module: image acquisition, parameter setting, trajectory track, background modeling, characteristic are calculated, the result formats and shows and preservation.
2, white mouse behavior determination experimental system as claimed in claim 1 is characterized in that: described casing (1) is the sound insulation casing.
3, white mouse behavior determination experimental system as claimed in claim 1 is characterized in that: described illuminator is array LED (6).
4, white mouse behavior determination experimental system as claimed in claim 3, it is characterized in that: described array LED (6) is evenly distributed on video input unit (3) all around.
5, white mouse behavior determination experimental system as claimed in claim 1 is characterized in that: described animal activity box (2) be positioned at video input unit (3) under.
6, white mouse behavior determination experimental system as claimed in claim 1 is characterized in that: described animal activity box (2) is made up of several boxes that shuttle back and forth (7).
7, white mouse behavior determination experimental system as claimed in claim 6, it is characterized in that: be provided with an active dividing plate in the middle of the described box that shuttles back and forth (7), the box (7) that will shuttle back and forth is divided into first box (8) and second box (9), wherein first box (8) around and the bottom surface be white, and the bottom surface is smooth, second box (9) is black with the bottom surface all around, and grid is engraved in the bottom surface.
8, as claim 1 or 7 described white mouse behavior determination experimental systems, it is characterized in that:
The step that described white mouse video tracking mensuration system carries out white mouse behavior videometry is as follows:
(I) system hardware that the white mouse video tracking is measured system is installed;
(II) systems soft ware that the white mouse video tracking is measured system is installed, the program of desire being carried out white mouse behavior videometry systems soft ware deposits in the program storage of computer (4);
(III) start the working procedure that the white mouse video tracking is measured system, the system hardware of the white mouse video tracking being measured system carries out initialization; Comprise the initialization of video input unit (3), image pick-up card, computer (4); The videometry visual field that the white mouse video tracking is measured system is set in being initialized as of video input unit (3), sets the sampling period that the white mouse video tracking is measured system;
(IV) video input unit (3) was gathered the Sports Series image in the videometry visual field and is write down corresponding acquisition time according to the sampling period of setting, and video input unit (3) is input to image pick-up card with the Sports Series view data that collects;
(V) image pick-up card carries out analog digital conversion to the Sports Series view data, becomes Digital Image Data, and Digital Image Data is transferred to computer (4);
The mode that white mouse video tracking mensuration system carries out white mouse behavior videometry comprises: the black area background modeling is measured mode and not modeling mensuration mode, carry out before the white mouse behavior videometry Sports Series view data that collects being estimated, when on estimating the Sports Series view data, urine being arranged, the artificial appointment adopted black area background modeling mensuration mode, this moment background preserve to take place and only occur in follow the trail of white mouse as a result the position in the white area, background is preserved back execution in step (VI); When on estimating the Sports Series view data, not having urine, artificial appointment is adopted the black area background modeling is measured mode or adopted not modeling to measure mode, background was preserved back execution in step (VI) when employing was measured mode to the black area background modeling, direct execution in step (VI) when adopting not modeling to measure mode;
(VI) tracing point of animal position in each frame of digital image calculates in white mouse video tracking mensuration system by the white mouse track algorithm;
(VII) the white mouse video tracking is measured the tracing point connection of system with white mouse position in each frame of digital image, generates the movement track figure of white mouse;
(VIII) the white mouse video tracking working procedure of measuring system calculates the characteristic of white mouse according to the movement track figure of white mouse, and the every trade of going forward side by side is for measuring;
(IX) computer (4) shows movement sequence image, movement track figure and the characteristic of white mouse and be stored among computer (4) data base.
Described white area be on the Digital Image Data with the corresponding zone of first box (8), described black area be on the Digital Image Data with the corresponding zone of second box (9).
9, white mouse behavior determination experimental system as claimed in claim 8, it is characterized in that: described white mouse track algorithm comprises the steps:
To each frame of digital image:
A. select tracing area;
B. according to the black area threshold value of setting and white area threshold value with black area in the tracing area and white area binaryzation;
C. adopt the black connected region of labeling algorithm labelling black area and white area line by line;
D. find out the maximum black connected region in black area and the white area, and calculate center of gravity, center of gravity is the tracing point of white mouse position;
Wherein, adopt the black area background modeling is measured mode, the black area threshold value was set to background modeling black area threshold value when background was preserved back execution in step (VI); Adopt not modeling to measure mode, directly during execution in step (VI), the black area threshold value is set to black area commonly used threshold value.
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