CN101579015B - Chromium compound nutrient yoghourt powdered milk and preparation method - Google Patents
Chromium compound nutrient yoghourt powdered milk and preparation method Download PDFInfo
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Abstract
The invention relates to chromium compound nutrient yoghourt powdered milk which has good adjuvant therapy effect on diabetes mellitus patients and comprises chromium yoghourt powdered milk powdered milk, solubility dietary fiber and xylitol. Compared with the existing hypoglycemia yogurt, the chromium compound nutrient yoghourt powdered milk powdered milk is convenient to store, transport, and carry, can be eaten at any time, and also keeps the advantages of the intrinsic hypoglycemia yogurt.
Description
Technical field
The present invention relates to a kind of dairy products, particularly a kind of suitable diabetic uses, diabetes are had good prevention and adjuvant treatment effect, have the intrinsic biological characteristics of active probiotic again simultaneously and are convenient to the chromium compound nutrient yoghourt powdered milk that stores, transport and carry.
Background technology
Diabetes are a kind of common endocrine metabolism diseases that have a strong impact on human health.Because nutrition treatment is the basic measures for the treatment of diabetes, therefore, make great efforts to explore the certain diabetes functional food of effect is the important topic that people study always.We had once developed a kind of hypoglycemic sour milk, and obtained national inventing patent, but liquid sour milk exist the storage time short, freight is high and carry shortcomings such as inconvenience, therefore, develop a kind of chromium compound nutrient yoghourt powdered milk of storing, carry, transporting be convenient to, make the nutraceutical of described milk powder as the diabetes patient, edible convenient.
Summary of the invention
The purpose of this invention is to provide a kind of chromium compound nutrient yoghourt powdered milk, this chromium compound nutrient yoghourt powdered milk is compared with existing hypoglycemic sour milk, be convenient to store, transport, carry and edible at any time, and kept the advantage of original hypoglycemic sour milk in addition.
Realize that technical scheme of the present invention is:
Be used to prepare the sour milk of rich chromic acid milk milk powder, it comprises raw milk, ferment agent for sour milk and additive, expression by weight percentage, and each constituent is as follows:
Raw milk 83.0～93.5%;
Ferment agent for sour milk 2.5～7.0%;
Additive:
Cobastab
10.01～0.02%;
Cobastab
20.01～0.02%;
Niacinamide 0.02～0.035%;
Calcium pantothenate 0.005～0.01%;
Chrome green 0.000913～0.00183%;
FOS 4～10%.
Be used to prepare the sour milk of rich chromic acid milk milk powder, technical scheme is preferably, and it is as follows that raw milk, ferment agent for sour milk and additive are respectively formed percentage by weight:
Raw milk 86.932383%;
Ferment agent for sour milk 5%;
Additive:
Cobastab
10.015%;
Cobastab
20.015%;
Niacinamide 0.030%;
Calcium pantothenate 0.00625%;
Chrome green 0.001367%;
FOS 8%.
Described raw milk is that milk or goat milk or milk powder are reduced to liquid milk.
Adopt the method for the rich chromic acid milk of above-mentioned yogurt production milk powder, following steps are arranged; Take by weighing 40～60 parts of rich chromic acid milk, 80～90 parts of whole milk powders mix; After 35 ℃～45 ℃ vacuum decompression dryings, it is 100 parts with the whole milk powder ad pond om again.
Prepare the method for rich chromic acid milk milk powder, method is preferably: take by weighing 45 parts of rich chromic acid milk, 90 parts of whole milk powders mix; After 42 ℃ of vacuum decompression dryings, it is 100 parts with the whole milk powder ad pond om again.
Chromium compound nutrient yoghourt powdered milk, it comprises rich chromic acid milk milk powder, soluble dietary fiber, xylitol, each constituent is as follows by weight percentage:
Rich chromic acid milk milk powder 75%-85%;
Soluble dietary fiber 10%-18%;
Xylitol 5%-7%.
Chromium compound nutrient yoghourt powdered milk, technical scheme is described rich chromic acid milk milk powder, soluble dietary fiber, xylitol preferably, each constituent is as follows by weight percentage:
Rich chromic acid milk drying powdered milk 80%;
Soluble dietary fiber 14%;
Xylitol 6%.
Cobastab
1, Cobastab
2, niacinamide, calcium pantothenate, Cr
2O
3, FOS all adopts Beijing Bright Eye Drops Food Co., Ltd everyday, can certainly adopt the commercially available product of using for food additives of the same name of other companies.
Soluble dietary fiber, xylitol adopt far-reaching Food Co., Ltd to produce, and also can adopt the commercially available product of the same name that supplies food additives to use of other companies.
Xylitol also can adopt other to allow the confectionery additive that uses.
The content of soluble dietary fiber is 80.0～100.0% by weight percentage in the soluble dietary fiber.
The lactobacillus inoculation (this bacterial classification is streptococcus thermophilus, 1: 1 freeze-dried mixed bacterial classification of Bulgarian lactic acid bacterium) that ferment agent for sour milk adopts the strong phase of Anhui Province's Huaibei City sunlight mountain dairy industry company to produce, bacterial classification is culture medium with milk, gets ferment agent for sour milk (according to product description activation culture bacterial classification) after the activated cultivation.With the ferment agent for sour milk that other lactobacillus inoculation (comprising Bifidobacterium Bifidum) obtains, prepare the sour milk that is used to prepare rich chromic acid milk milk powder of the present invention, can reach the described effect of invention equally.
Beneficial effect of the present invention
1. this chromium compound nutrient yoghourt powdered milk has the intrinsic biological characteristics of active probiotic, and concrete experimentation and result are as follows:
(1) different baking temperatures prepare chromium compound nutrient yoghourt powdered milk: get rich chromic acid milk 40g, whole milk powder 90g, mixing, by with 3 parts of quadrat method preparations, respectively in vacuum drying chamber with 30 ℃, 42 ℃, 80 ℃ drying under reduced pressure to moisture less than 6% (30 ℃, 42 ℃, 80 ℃ drying under reduced pressure to moistures need 13h, 7h and 4h respectively less than 6%).Respectively with whole milk powder ad pond om 100g, be 30 ℃, 42 ℃, 80 ℃ rich chromic acid milk milk powder again.Prepare chromium compound nutrient yoghourt powdered milk again, that is: get rich chromic acid milk milk powder 80g, soluble dietary fiber 14g, the xylitol 6g of above-mentioned different baking temperature preparations respectively, be different baking temperature chromium compound nutrient yoghourt powdered milks after the mixing.
(2) the chromium compound nutrient yoghourt powdered milk viable count testing result of different baking temperature preparations
The comparison (cfu/g) of the chromium compound nutrient yoghourt powdered milk viable count of the different baking temperature preparations of table 1
Annotate: physics and chemistry and sanitary index detect and meet the GB19644 requirement
(3) chromium compound nutrient yoghourt powdered milk with different baking temperature preparations directly prepares rich chromium composite yoghourt: the chromium compound nutrient yoghourt powdered milk of getting different baking temperature preparations mixes with 1: 6 weight with water (40-45 ℃ of warm boiling water), prepares rich chromium composite yoghourt according to 42 ℃ of direct fermentations of common sour milk fermentation condition.Basic condition sees Table 2;
The chromium compound nutrient yoghourt powdered milk of the different baking temperature preparations of table 2 directly prepares the result of rich chromium composite yoghourt
| 30 ℃ of dry chromium compound nutrient yoghourt powdered milks prepare rich chromium composite yoghourt | 42 ℃ of dry chromium compound nutrient yoghourt powdered milks prepare rich chromium composite yoghourt | 80 ℃ of dry chromium compound nutrient yoghourt powdered milks prepare rich chromium composite yoghourt | |
| Fermentation time | 10h | 6h | 10h |
| Whether form solidification type yoghourt | Be | Be | Not |
| The viable lactic acid bacteria number | 1.3×10 7/g | 1.1×10 9/g | Do not detect |
| Lactic acid content | 0.7％ | 0.96％ | Do not detect |
This chromium compound nutrient yoghourt powdered milk have the composite liquefied sour milk of Fu Ge same improve the diabetic sugar metabolism, reason is the chrome green that chromium compound nutrient yoghourt powdered milk adds, and can assist insulin to keep normal glycometabolism; The Cobastab that adds
1, Cobastab
2, niacinamide, calcium pantothenate etc. be several important coenzyme compositions of pyruvate dehydrogenase complex that play a significant role in the breakdown of glucose metabolism; Contain high-activity lactic acid bacteria, FOS and soluble dietary fiber in the chromium compound nutrient yoghourt powdered milk and good action is arranged keeping normal probio of diabetic's enteron aisle and function of intestinal canal.
Because 42 ℃ of dry required times of dry chromium compound nutrient yoghourt powdered milk are shorter, viable lactic acid bacteria is counted height, directly the required fermentation time of preparation sour milk is short, for this reason, we have carried out the experimental study of 42 ℃ of dry chromium compound nutrient yoghourt powdered milks (hereinafter to be referred as chromium compound nutrient yoghourt powdered milk) to the influence of diabetes rat glycometabolism, and main result is as follows:
(1) animal grouping and nursing:
168 of Wistar rats are got 24 at random by body weight and are normal control group (I group, male and female half and half), and all the other 144 is model observation group (wherein 24 are the prophylactic treatment group, i.e. the VI group).Feed on the basis at conventional feed, prophylactic treatment group rat gives chromium compound nutrient yoghourt powdered milk solution and irritates stomach, other rat oral gavage distilled water, once a day, behind the continuous irrigation stomach 14 days, model observation group rat tail vein is injected fresh alloxan solution 50mg/ (k.w), makes diabetes model, normal control group tail vein injection isotonic saline solution.Measured fasting blood-glucose behind the injection alloxan on the 7th day, with the rat of blood glucose value 〉=13.8mmol/L as the diabetes model rat.Getting 8 at random except that the prophylactic treatment group becomes the mould rat, the one-tenth mould rat of other model observation group of non-prophylactic treatment group is divided into diabetes control group (II group), rich Cr materials formula milk group (III group), rich chromium composite yoghourt group (IV group), rich chromium composite yoghourt drying powdered milk group (V group), 8 every group at random by blood sugar.On the animal ingestion conventional feed and the basis of freely drinking water, the I treated animal is irritated stomach distilled water, the II treated animal is irritated stomach active probiotic common sour milk milk power solution, the III treated animal is irritated the rich Cr materials formula milk of stomach solution, the IV treated animal is irritated the rich chromium composite yoghourt of stomach, and the V treated animal is irritated the rich chromium composite yoghourt of stomach low temperature drying milk power solution, and the VI treated animal continues to irritate the rich chromium composite yoghourt of stomach drying powdered milk solution, once a day, the continuous irrigation stomach is 90 days.
(2) zoopery MAIN OUTCOME MEASURES
1. the mensuration of blood sugar level: measure respectively at carrying out fasting blood-glucose in the 37th day, the 67th day, the 97th day behind the injection ALX, carried out two hours carbohydrate tolerance tests behind the injection ALX in the 39th day, the 99th day.
2. experimental rat is respectively organized in execution in the 101st day behind injection ALX, and it is centrifugal to get whole blood, carries out serum levels of triglyceride, T-CHOL, low-density lipoprotein, HDL, urea nitrogen, creatinine, GSP, MDA content and T-AOC, SOD determination of activity.
(3) experimental result
1. inject behind the ALX the 7th day, the 37th day, the 67th day, the 97th day result of blood sugar on an empty stomach:
Behind the injection ALX the 37th, 67,97 day, II-VI group was respectively organized rat fasting blood-glucose all more than 13.8mmol/L, and highly significant is higher than I group (P＜0.01); III-VI group rat fasting blood-glucose all is lower than the II group, and there is significant difference (P＜0.05) in 37th, 67 days, 97 days VI group between organizing with II, the 97th day, IV, V group rat fasting blood-glucose also significantly or highly significant be lower than II group (P＜0.05 or P＜0.01).The results are shown in Table 3.
The measurement result of table 3 rat fasting blood-glucose (mmol/L X ± S)
Compare * * P＜0.01, * P＜0.05 with the I group; Compare with the II group ▲ ▲ P＜0.01, ▲ P＜0.05;
Compare #P＜0.05 with the III group
2. inject the 39th day, the 99th day two hours carbohydrate tolerance test results behind the ALX:
See Table 1 as seen, behind the injection ALX the 39th day II-VI group respectively organize rat sugar tolerance 0.5h, 1.0h, 1.5h, 2.0h each the time picture point blood sugar detection value all highly significant be higher than I group (P＜0.01), though the III-VI treated animal above each the time picture point blood sugar detection value be lower than the II group, have remarkable or highly significant difference (P＜0.05 or P＜0.01) between the blood sugar detection value of rarely seen VI treated animal picture point when picture point and IV treated animal are at 1.5h when 0.5h, 1.5h and the II group.
The 99th day, IV-VI group rat above-mentioned each the time picture point blood sugar detection value also significantly or highly significant be lower than II, III group (P＜0.05 or P＜0.01).
Table 4 is respectively organized measurement result (the mmol/L X ± S) of rat sugar tolerance
The 99th day two hours carbohydrate tolerance test results behind the injection ALX
Compare * * P＜0.01, * P＜0.05 with the I group; Compare with the II group ▲ ▲ P＜0.01, ▲ P＜0.05
Compare ##P＜0.01, #P＜0.05 with the III group
3. rat saccharification haemocyanin level determination result:
When experiment finishes the II-VI group respectively organize rat GSP content all highly significant be higher than I group (P＜0.01), but IV-VI group rat significantly or highly significant be lower than II, III group (P＜0.05 or P＜0.01).The results are shown in Table 5.
Table 5 is respectively organized measurement result (the mg/ml X ± S) of rat saccharification haemocyanin
Compare * * P＜0.01, * P＜0.05 with the I group; Compare with the II group ▲ ▲ P＜0.01, ▲ P＜0.05;
Compare ##P＜0.01, #P＜0.05 with the III group
4. rat blood serum TG, TC, LDL, HDL, BUN, CREA measurement result
Table 4 as seen, III when experiment finishes～VI group rat blood serum TG, TC, LDL, BUN, CREA content all are lower than the II group, HDL content is higher than the II group, and exists significantly or highly significant difference (P＜0.05 or P＜0.01) between IV-VI group rat blood serum TG, TC, HDL and the II group.Exist significantly or highly significant difference (P＜0.05 or P＜0.01) between IV-VI group rat blood serum CREA and the II group.
Table 6 is respectively organized rat fat and renal function measurement result (mmol/L X ± S)
Compare * * P＜0.01, * P＜0.05 with the I group; Compare with the II group ▲ ▲ P＜0.01, ▲ P＜0.05;
Compare ##P＜0.01, #P＜0.05 with the III group
5. rat blood serum T-AOC, SOD vigor and MDA assay result:
When experiment finishes the II-VI group respectively organize rat blood serum T-AOC, SOD vigor all significantly or highly significant be lower than I group (P＜0.05 or P＜0.01), but IV group, V group, VI group significantly or highly significant be higher than II group (P＜0.05 or P＜0.01); II-VI group Content of MDA all is higher than the I group, and deposits significantly or highly significant difference (P＜0.05 or P＜0.01) between II-V group and the I group; Though III-VI group Content of MDA all is lower than the II group, does not have significant difference.The results are shown in Table 7.
Table 7 each experimental group rat blood serum T-AOC, SOD and MDA measurement result (X ± S)
Compare * * P＜0.01, * P＜0.05 with the I group; Compare with the II group ▲ ▲ P＜0.01, ▲ P＜0.05;
Conclusion:
(1) takes in chromium compound nutrient yoghourt powdered milk in advance and can alleviate the damaging action of alloxan, and have the latent effect of certain prevention diabetes rat.
(2) as seen by rat fasting blood-glucose, sugar tolerance, saccharification haemocyanin measurement result, chromium compound nutrient yoghourt powdered milk is the same with chromium compound nutrient yoghourt, can both significantly improve diabetes rat glycometabolism due to the alloxan, it is unusual to improve due to the antioxidant ability of organism of diabetes model rat and the alloxan diabetes model rat fat, and rich Cr materials formula milk does not have positive effect.Show that the above-mentioned effect of adding in chromium compound nutrient yoghourt and the chromium compound nutrient yoghourt powdered milk prescription that multiple components produced is relevant with probiotics fermention.
(3) because chromium compound nutrient yoghourt powdered milk contains the probio of high bioactivity, have the same local flavor characteristics of chromium compound nutrient yoghourt.
The present invention has the multiple eating method, promptly can directly take after mixing it with water according to the eating method of ordinary powdered milk, also can make sour milk.When making sour milk, do not need the bacterial classification of special equipment and fermentation condition and fermentation, only need chromium compound nutrient yoghourt powdered milk and water to remain on 35～45 ℃ according to must ratio mixing, the 3～6h that ferments can be made into solidification type yoghourt.Therefore compare with existing hypoglycemic sour milk, have and be convenient to store, conveniently transport and carry, the advantage that can eat at any time.In addition, in transportation and storage process, with respect to sour milk, its transportation volume reduces greatly, therefore also has transportation and the low advantage of storage cost.
The present invention adopts the sour milk that is exclusively used in the rich chromic acid milk of preparation milk powder to prepare the rich chromic acid milk of chromium compound nutrient yoghourt powdered milk milk powder; can guarantee that chromium compound nutrient yoghourt powdered milk can keep the advantage of original hypoglycemic sour milk; have the effect of protecting islet function, improving glycometabolism and reduction blood pressure and blood lipoid equally, the diabetic is had the effect of good supplemental treatment.
The specific embodiment
1. preparation is used to prepare the sour milk of rich chromic acid milk powder
The sour milk that is exclusively used in the rich chromic acid milk powder of preparation of embodiment 1-4, each constituent content is as shown in table 8:
Each constituent content (unit: gram) of the rich chromic acid milk of table 8
| Embodiment | Milk (g) | Cobastab 1(g) | Cobastab 2(g) | Niacinamide (g) | Calcium pantothenate (g) | Chrome green (g) | FOS (g) | Lactobacillus yogurt leavening (g) |
| 1 | 934.549087 | 0.100 | 0.100 | 0.200 | 0.050 | 0.000913 | 40.0 | 25 |
| 2 | 904.423304 | 0.125 | 0.125 | 0.250 | 0.065 | 0.011696 | 60.0 | 35 |
| 3 | 869.32383 | 0.150 | 0.150 | 0.300 | 0.063 | 0.013670 | 80.0 | 50 |
| 4 | 839.131700 | 0.200 | 0.200 | 0.350 | 0.100 | 0.018300 | 100.0 | 60 |
Take in the raw material of the sour milk of the rich chromic acid milk powder of preparation by table 8 prescription, prepare according to following method: get milk (or add water by 1: 6 ratio be recovered to liquid milk) filtration with the special-purpose milk powder of producing sour milk, 80 ℃～100 ℃ sterilizations 5～15 minutes, add described other each nutritional labeling of table 8, sterilization is 2～5 minutes again; Batching milk after the above-mentioned sterilization is cooled to 38 ℃～45 ℃, adds lactic acid bacteria fermenting agent, stir evenly, 35 ℃～45 ℃ ferment to curdy, promptly get the sour milk that is used to prepare rich chromic acid milk powder of the present invention.
2. prepare rich chromic acid milk milk powder:
Rich chromic acid milk each constituent content of milk powder of table 9 and preparation
| Embodiment | Rich chromic acid milk (g) | Whole milk powder (g) | Baking temperature (℃) | Dry back adds to (g) with whole milk powder |
| 5 | 40 | 90 | 35、42、80 | 100 |
| 6 | 50 | 90 | 35、42、80 | 100 |
| 7 | 60 | 90 | 35、42、80 | 100 |
Annotate: baking temperature: three kinds of baking temperatures of each embodiment are respectively 35 ℃, 42 ℃, 80 ℃.
Take by weighing rich chromic acid milk, whole milk powder according to table 9, mix; After 35～42 ℃ of vacuum decompression dryings, be 100 parts (grams) with the whole milk powder ad pond om again.
3. get the described rich chromic acid milk milk powder of step 2, get rich chromic acid milk milk powder, xylitol, soluble dietary fiber, xylitol by the described proportioning of table 10, mixing promptly gets rich chromic acid milk milk powder of the present invention.
Each constituent content of the rich chromic acid milk of table 10 milk powder
| Embodiment | Rich chromic acid milk milk powder (g) | Soluble dietary fiber (g) | Xylitol (g) |
| 1 | 75 | 18 | 7 |
| 2 | 80 | 14 | 6 |
| 3 | 85 | 10 | 5 |
Need when edible,, get milk powder and add a certain proportion of boiling water and directly take after mixing it with water and get final product according to eater's hobby.
When needing to eat sour milk, get chromium compound nutrient yoghourt powdered milk 15～20g of the present invention, add about 42 ℃ warm boiling water to cumulative volume 100mL, press the Yoghourt fermentation condition at 35～45 ℃, fermentation 3～6h can be made into the rich chromium composite yoghourt of coagulating type.
Chromium compound nutrient yoghourt powdered milk of the present invention is suitable for diabetic to take, and diabetes patient's amount every day is 30-50g, and good adjuvant treatment effect is arranged.
Claims (4)
1. the preparation method of a rich chromic acid milk milk powder is characterized in that: the sour milk that is used to prepare rich chromic acid milk milk powder prepares with the whole milk powder vacuum decompression is dry, and following steps are arranged:
Take by weighing the sour milk and the whole milk powder that are used to prepare rich chromic acid milk milk powder,
Be used to prepare 40～60 parts of the sour milks of rich chromic acid milk milk powder
80～90 parts of whole milk powders
Mix; After 35 ℃～45 ℃ vacuum decompression dryings, it is 100 parts with the whole milk powder ad pond om again;
The described sour milk that is used to prepare rich chromic acid milk milk powder comprises raw milk, ferment agent for sour milk and additive, expression by weight percentage, and each constituent is as follows: raw milk 83.0～93.5%; Ferment agent for sour milk 2.5～7.0%; Additive: Cobastab
10.01～0.02%; Cobastab
20.01～0.02%; Niacinamide 0.02～0.035%; Calcium pantothenate 0.005～0.01%; Chrome green 0.000913～0.00183%; FOS 4～10%.
2. according to right 1 described method, it is characterized in that: take by weighing the sour milk and the whole milk powder that are used to prepare rich chromic acid milk milk powder,
Be used to prepare 45 parts of the sour milks of rich chromic acid milk milk powder, 90 parts of whole milk powders
Mix; After 42 ℃ of vacuum decompression dryings, it is 100 parts with the whole milk powder ad pond om again.
3. chromium compound nutrient yoghourt powdered milk is characterized in that it is made up of soluble dietary fiber, xylitol, the described rich chromic acid milk milk powder of claim 1, and each constituent is as follows by weight percentage:
Rich chromic acid milk milk powder 75%-85%;
Soluble dietary fiber 10%-18%;
Xylitol 5%-7%.
4. chromium compound nutrient yoghourt powdered milk according to claim 3 is characterized in that described rich chromic acid milk milk powder, soluble dietary fiber, xylitol, and each constituent is as follows by weight percentage:
Rich chromic acid milk milk powder 80%;
Soluble dietary fiber 14%;
Xylitol 6%.
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