CN101538541A - Culture method of biological cleaning strains in low-concentration volatile mixed organic waste gas - Google Patents

Culture method of biological cleaning strains in low-concentration volatile mixed organic waste gas Download PDF

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CN101538541A
CN101538541A CN200910039089A CN200910039089A CN101538541A CN 101538541 A CN101538541 A CN 101538541A CN 200910039089 A CN200910039089 A CN 200910039089A CN 200910039089 A CN200910039089 A CN 200910039089A CN 101538541 A CN101538541 A CN 101538541A
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bacterial classification
waste gas
strains
organic waste
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CN101538541B (en
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左红影
林晓生
程汉林
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Tiehan Environmental Protection Group Co., Ltd.
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Guangzhou Huanfa Environmental Protection Engineering Co Ltd
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Abstract

The invention relates to a culture method of biological cleaning strains in low-concentration volatile mixed organic waste gas. The method comprises the following steps: a), culturing a first strain; b), carrying out the combined inoculation of the first strain and a second strain and using tap water to prepare the sludge concentration of the mixed strains; and c), using mixed organic matter in the waste gas as a substrate to be cast into an insition culture medium so as to culture and train insitions. The first strain is prepared by culturing and training from activated mud taken from a printing and dyeing sewage treatment plant and is a mixed strain of flavobacterium and Bacillus cereus, and the second strain is prepared by collecting activated mud collected from a secondary sedimentation tank of a petrochemical sewage treatment plant and is a mixed strain comprising the flavobacterium, the Bacillus cereus, xylose oxidase Achromobacter and Pseudomonas stutzeri. The method facilitates the balanced growth of various cultured microorganisms and can simultaneously decompound various target configuration compounds at high efficiency.

Description

A kind of method of cultivation of biological cleaning strains in low-concentration volatile mixed organic waste gas
Technical field
The invention belongs to biological inoculum and cultivate technical field, be specifically related to a kind of method of cultivation of biological cleaning strains in low-concentration volatile mixed organic waste gas.
Background technology
Topsoil is one of at present the most outstanding environmental problem.The industrial gaseous waste of volatile organic compounds is the atmospheric polluting material of discharging general, most species widest in area except that dust.Biological method purification low-concentration industrial waste gas is a kind of novel method of industrial waste gas purifying, the history that extensive studies and application were only had an appointment 20 years.This method be a kind of with microorganism to pollutent have more by force, the characteristics of adaptive faculty faster, it is tamed, making microorganism can be the carbon source and the energy with the target contaminant, and pollutent is converted into harmless, simple material (as carbonic acid gas, water etc.).Microorganism is easier to for the degraded ratio of one matter in the exhaust gas biological purifying system, and microorganism participates in because of the degraded that contains more compound substance then needs multiple microorganism for mix waste gas through taming the degraded of easy adaptation one matter.But the but difficult control of the growth balance between the multiple microorganism has only single certain microorganism growth to preponderate so cause easily.Therefore, for the detergent power of the biological purification system of the multiple organic compound industrial gaseous waste that mixes a little less than.
Summary of the invention
The method of cultivation that the purpose of this invention is to provide a kind of biological cleaning strains in low-concentration volatile mixed organic waste gas makes balanced growth between the multiple microorganism, cultivates the composite biological inoculum of efficient degradation plurality of target component simultaneously.
The cultivation operation of this biological cleaning strains in low-concentration volatile mixed organic waste gas is carried out at normal temperatures and pressures, adopts aerobic vibration flask culture method.It may further comprise the steps:
1) cultivates bacterial classification one;
2) bacterial classification one and bacterial classification two combined inoculations, and the sludge concentration of allocating mixed strains with tap water;
3) be substrate to mix organism in the waste gas, be dosed in the inoculum substratum, inoculum is cultivated domestication;
Wherein, this bacterial classification one is taken from active sludge gained after cultivating domestication of dyeing and printing sewage treatment plant, is the mixed strains of Flavobacterium and bacillus cereus genus; This bacterial classification two is for gathering active sludge in the second pond of taking from petrifaction sewage treatment plant, and it is the mixed strains that comprises Flavobacterium, bacillus cereus genus, Achromobacter xylosoxidans and Pseudomonas stutzeri.
This bacterial classification two is 20: 1 with the volume ratio of bacterial classification one combined inoculation.
Further, cultivating bacterial classification one comprises the steps:
A) liquid nutrient medium of preparation bacterial classification one: get phosphate buffered saline buffer, MgSO 4Solution, CaCl 2Solution, FeCl 3Solution and trace element solution mix, and add distilled water diluting, and adjust pH value, through high pressure steam sterilization, aseptic subpackaged after, add liquid paraxylene and diesel oil as the sole carbon source and the energy, the sealing preservation;
B) domestication: the abundant aeration activation of active sludge intermixture that will take from dyeing and printing sewage treatment plant, then handling with ultrasonator is uniformly dispersed the mud flco, get the mud mixed liquid of handling well and add in the screening domestication substratum, adopt the shaking table test that microorganism is carried out aerobic shaking culture;
C) with the inoculation of screening in the activation enrichment medium, after the aerobic vibration, place supercentrifuge centrifugal, then with phosphate buffered saline buffer clean, centrifugal, the thalline of centrifugal gained is made bacterial classification suspension, promptly get bacterial classification one.
Further, the composition of the liquid nutrient medium of described bacterial classification one is: phosphate buffered saline buffer: K 2HPO 4H 2O 20～25g/L, KH 2PO 46～9g/L, Na 2HPO 412H 2O 30～35g/L, NH 4Cl 3～6g/L; MgSO 4Solution: MgSO 420～25g/L; CaCl 2Solution: CaCl 235～40g/L; FeCl 3Solution: FeCl 30.1～0.3g/L; Trace element solution: MnSO 4H 2O35～40mg/L, ZnSO 4H 2O 40～45mg/L, (NH 4) 6Mn 7O 244H 2O 30～35mg/L.
Further, the activation enrichment culture based component of described cultivation bacterial classification one is extractum carnis 5.0g, peptone 10g, and NaCl 5g, water 1000mL, and with its autoclaving.
Further, the composition to the inoculum substratum of the inoculum of described bacterial classification one and bacterial classification two domestication is: hydrolyzed starch 0.0～1.0g/L, urea 0.5～1g/L, KH 2PO 40.2～0.3g/L, Na 2HPO 40.2～0.3g/L, MgSO 47H 2O 0.1～0.2g/L, CaCl 20.02～0.03g/L, vitamins B 10.05～0.15g/L.
Inoculum is cultivated the domestication initial stage, with hydrolyzed starch concentration be the 1.0g/L substratum as the sole carbon source and the energy, and add N, P, Mg, Ca, Fe nutritive element.After cultivating the end of domestication initial stage, reduce the starch consumption in the substratum gradually, increase exhausted air quantity; Every 48 hours, stop air inlet and vibration, staticly settled 2-3 hour, get rid of supernatant liquor, and add the inoculum substratum.
Balanced growth between the multiple microorganism of cultivating according to the inventive method, efficient degradation plurality of target component mixture simultaneously.
Description of drawings
Fig. 1 is that the present invention mixes organic exhaust gas biological purifying process flow sheet.
Embodiment
The cultivation operation of this light-concentration volatile volatile mixed organic exhaust gas biological cleaning strains is carried out at normal temperatures and pressures, adopts aerobic vibration flask culture method.It comprises preparation bacterial classification one, inoculation operation and cultivates three steps of domestication.
(1) preparation bacterial classification one.It can be divided into three sub-steps.
(a) liquid nutrient medium of preparation bacterial classification one.
Get phosphate buffered saline buffer 5.0mL, MgSO 4Solution 3.0mL, CaCl 2Solution 1.0mL, FeCl 3Solution 1.0mL, trace element solution 1.0mL mixes, and adds distilled water diluting to 1.0L, and the pH value is adjusted to 7.0-7.2.Through high pressure steam sterilization, aseptic subpackaged after, add 0.5g liquid paraxylene and 0.5 diesel oil, as the sole carbon source and the energy, sealing is preserved.
Wherein, the composition of each solution of liquid nutrient medium of this preparation bacterial classification one is: phosphate buffered saline buffer (K 2HPO 4H 2O22.0g/L, KH 2PO 47.6g/L, Na 2HPO 412H 2O31.8g/L, NH 4Cl 4.0g/L); MgSO 422.0g/L; CaCl 236.0g/L; FeCl 30.15g/L; Trace element solution (MnSO 4H 2O 37.0mg/L, ZnSO 4H 2O 41.0mg/L, (NH 4) 6Mn 7O 244H 2O 32.0mg/L.
(b) domestication and screening.
At first will take from the abundant aeration activation of the active sludge intermixture 24h of Guangzhou one cotton spinning company, and then handle 20min the mud flco is uniformly dispersed with ultrasonator.Get the mud mixed liquid 5mL that handles well and add in the screening domestication substratum, adopt the shaking table test microorganism to be carried out aerobic shaking culture, the shaking table temperature: 25～30 ℃, rotating speed 110r/min.Acclimation period is 3 days, and the domestication number of times is 6 times.When each acclimation period finishes, get the 5.0mL nutrient solution and be connected in the fresh screening domestication substratum.
(c) preparation bacterial classification one.
With the screening inoculation in the activation enrichment medium in, behind the aerobic vibration 48h, place the centrifugal 10min of supercentrifuge (8000r/min), clean with phosphate buffered saline buffer then, centrifugal 10min (10000r/min) makes bacterial classification suspension with the thalline of centrifugal gained, is bacterial classification one.This bacterial classification one is that Flavobacterium (Elavobacterium) and bacillus cereus belong to the mixed strains of (Bacilluscereus).
Wherein, activation enrichment culture based formulas is: extractum carnis 5.0g, and peptone 10g, NaCl5g, water 1000mL, and with its autoclaving.
(2) inoculation operation.
From the second pond of petrochemical plant sewage work, gather active sludge, as bacterial classification two.Bacterial classification two is to belong to (Bacillus cereus), Achromobacter xylosoxidans (Achromobacterxylosoxidans) with Flavobacterium (Elavobacterium), bacillus cereus, and Pseudomonas stutzeri (Pseudomonas stutzeri) is main mixed strains.
Wherein, the sludge concentration of bacterial classification two (MLSS) is 15g/L.
Be quantitatively to get bacterial classification two and bacterial classification one at 20: 1 by volume, place in the vibration bottle that making its sludge concentration with tap water allotment mixed strains is 3g/L.
(3) cultivate domestication
To mix organism in the waste gas is substrate, the inoculum substratum of batching, and adopt to increase gradually and mix the organic exhaust gas method to inoculum cultivation domestication.
Cultivate the domestication initial stage (5 days), with hydrolyzed starch concentration be the 1.0g/L substratum as the sole carbon source and the energy, and add nutritive elements such as N, P, Mg, Ca, Fe.
After cultivating the end of domestication initial stage, reduce the starch consumption in the substratum gradually, increase exhausted air quantity.Every 48 hours, stop air inlet and vibration, staticly settled 2-3 hour, get rid of supernatant liquor, add the inoculum substratum.Substratum dosage: 5 ‰ (mass ratioes).
First 5 days, add the substratum that hydrolyzed starch concentration is 0.6g/L, press the 200mg/L air inlet; Second 5 days, add the substratum that hydrolyzed starch concentration is 0.3g/L, press the 600mg/L air inlet; The 3rd 10 days, add the substratum that hydrolyzed starch concentration is 0.0g/L, press the 1200mg/L air inlet.
Wherein, cultivated acclimation period 25 days.
The prescription of described inoculum substratum is: and hydrolyzed starch (0.0g/L, 0.3g/L, 0.6g/L, 1.0g/L), urea (1.0g/L), KH 2PO 4(0.25g/L), Na 2HPO 4(0.25g/L), MgSO 47H 2O (0.15g/L), CaCl 2(0.025g/L), vitamins B 1(0.1g/L).
See also Fig. 1, it is a volatile mixed organic exhaust gas biological purifying process flow diagram.Volatile mixed organic exhaust gas then enters and carries out pre-treatment in the pretreater that contains immobilized photocatalytic reaction device by the gas sampling systematic collection, enters then to mix organic exhaust gas biological purifying device, and this device contains charcoal fiber biological adsorption unit simultaneously.The bacterial classification that present embodiment is cultivated after taming drops in the circulating water channel, and the ON cycle water pump increases actual off gas treatment amount gradually simultaneously, and the results of regular determination biofilm packing is imported and exported waste gas and formed the removal efficient of calculating target processing thing.This test experiments continues 53 days.
Test-results as shown in Table 1.
Table volatile mixed organic exhaust gas biopurification result
Figure A20091003908900071
Annotate: off gas treatment amount Q=0.14m during mensuration 3/ h; Analytical procedure: vapor-phase chromatography.Monitoring department: China Petrochemical Corp. Guangzhou Branch inspection center.
The bacterial classification that the biological cleaning strains in low-concentration volatile mixed organic waste gas method of cultivation is cultivated according to the present invention, batch to and mix in organic exhaust gas biological purifying device, can cultivate in a short time and tame out and can handle the high-performance bio film of multiple volatile organic matter effectively simultaneously, thereby solve the improvement difficult problem of mix waste gas effectively.Adopt this bacterial classification one as inoculum, the mixing organic exhaust gas is efficiently purified, purified gas reaches discharging standards, and wherein benzene,toluene,xylene is removed efficient above 95%, and NMHC is removed efficient and surpassed 90%.This bacterial classification one as inoculum, is mixed organic exhaust gas biological purifying device after a biofilm success, do not need concrete management.This inoculum can resist stronger load impact, has the intermittence work capacity, can be when the organic exhaust gas biological purifying device of mixing to move in 7 days out of service once more, and its efficient is unaffected.After inoculating this inoculum, purification system only needs a small amount of bacterial classification of regular replenishment, N, P nutritive medium, and the purification system material consumption is little, and energy consumption is low, economic operating cost.
The above, it only is preferred embodiment of the present invention, be not that the present invention is done any pro forma restriction, though the present invention discloses as above with preferred embodiment, yet be not in order to limit the present invention, any those skilled in the art, in not breaking away from the technical solution of the present invention scope, when the technology contents that can utilize above-mentioned announcement is made a little change or is modified to the equivalent embodiment of equivalent variations, in every case be not break away from the technical solution of the present invention content, according to technical spirit of the present invention to any simple modification that above embodiment did, equivalent variations and modification all still belong in the scope of technical solution of the present invention.

Claims (10)

1. the method for cultivation of a biological cleaning strains in low-concentration volatile mixed organic waste gas may further comprise the steps:
A) cultivate bacterial classification one;
B) bacterial classification one and bacterial classification two combined inoculations, and the sludge concentration of allocating mixed strains with tap water;
C) be substrate to mix organism in the waste gas, be dosed in the inoculum substratum, inoculum is cultivated domestication;
Wherein, this bacterial classification one is taken from active sludge gained after cultivating domestication of dyeing and printing sewage treatment plant, is the mixed strains of Flavobacterium and bacillus cereus genus;
This bacterial classification two is for to gather active sludge in the second pond of petrifaction sewage treatment plant, it is the mixed strains that comprises Flavobacterium, bacillus cereus genus, Achromobacter xylosoxidans and Pseudomonas stutzeri.
2. the method for cultivation of biological cleaning strains in low-concentration volatile mixed organic waste gas as claimed in claim 1 is characterized in that, cultivates bacterial classification one and comprises the steps:
A) liquid nutrient medium of preparation bacterial classification one: get phosphate buffered saline buffer, MgSO 4Solution, CaCl 2Solution, FeCl 3Solution and trace element solution mix, and add distilled water diluting, and adjust pH value, through high pressure steam sterilization, aseptic subpackaged after, add liquid paraxylene and diesel oil as the sole carbon source and the energy, the sealing preservation;
B) domestication: the abundant aeration activation of active sludge intermixture that will take from dyeing and printing sewage treatment plant, then handling with ultrasonator is uniformly dispersed the mud flco, get the mud mixed liquid of handling well and add in the screening domestication substratum, adopt the shaking table test that microorganism is carried out aerobic shaking culture;
C) with the inoculation of screening in the activation enrichment medium, after the aerobic vibration, place supercentrifuge centrifugal, then with phosphate buffered saline buffer clean, centrifugal, the thalline of centrifugal gained is made bacterial classification suspension, promptly get bacterial classification one.
3. the method for cultivation of biological cleaning strains in low-concentration volatile mixed organic waste gas as claimed in claim 2 is characterized in that, the composition of the liquid nutrient medium of described bacterial classification one is:
Phosphate buffered saline buffer: K 2HPO 4H 2O 20～25g/L, KH 2PO 46～9g/L, Na 2HPO 412H 2O 30～35g/L, NH 4Cl 3～6g/L;
MgSO 4Solution: MgSO 420～25g/L;
CaCl 2Solution: CaCl 235～40g/L;
FeCl 3Solution: FeCl 30.1～0.3g/L;
Trace element solution: MnSO 4H 2O 35～40mg/L, ZnSO 4H 2O 40～45mg/L, (NH 4) 6Mn 7O 244H 2O30～35mg/L.
4. the method for cultivation of biological cleaning strains in low-concentration volatile mixed organic waste gas as claimed in claim 3, it is characterized in that: the optimal components of the liquid nutrient medium of described bacterial classification one is:
Phosphate buffered saline buffer: K 2HPO 4H 2O 22.0g/L, KH 2PO 47.6g/L, Na 2HPO 412H 2O 31.8g/L, NH 4Cl4.0g/L;
MgSO 4Solution: MgSO 422.0g/L;
CaCl 2Solution: CaCl 236.0g/L;
FeCl 3Solution: FeCl 30.15g/L;
Trace element solution: MnSO 4H 2O 37.0mg/L, ZnSO 4H 2O 41.0mg/L, (NH 4) 6Mn 7O 244H 2O32.0mg/L.
5. the method for cultivation of biological cleaning strains in low-concentration volatile mixed organic waste gas as claimed in claim 3, it is characterized in that: described activation enrichment culture based component is extractum carnis 5.0g, peptone 10g, NaCl 5g, water 1000mL, and with its autoclaving.
6. as the method for cultivation of claim 1～3,5 described biological cleaning strains in low-concentration volatile mixed organic waste gas, it is characterized in that, composition to the inoculum substratum of the inoculum of described bacterial classification one and bacterial classification two domestication is: hydrolyzed starch 0.0～1.0g/L, urea 0.5～1g/L, KH 2PO 40.2～0.3g/L, Na 2HPO 40.2～0.3g/L, MgSO 47H 2O 0.1～0.2g/L, CaCl 20.02～0.03g/L, vitamins B 10.05～0.15g/L.
7. the method for cultivation of biological cleaning strains in low-concentration volatile mixed organic waste gas as claimed in claim 6 is characterized in that: this bacterial classification two is 20: 1 with the volume ratio of bacterial classification one combined inoculation.
8. the method for cultivation of biological cleaning strains in low-concentration volatile mixed organic waste gas as claimed in claim 7, it is characterized in that: the optimal components to the inoculum substratum of the inoculum of described bacterial classification one and bacterial classification two domestication is: hydrolyzed starch 1.0g/L, urea 1.0g/L, KH 2PO 40.25g/L, Na 2HPO 40.25g/L, MgSO 47H 2O 0.15g/L, CaCl 20.025g/L, vitamins B 10.1g/L 0.01g/L.
9. the method for cultivation of biological cleaning strains in low-concentration volatile mixed organic waste gas as claimed in claim 8, it is characterized in that: inoculum is cultivated the domestication initial stage, with hydrolyzed starch concentration be the 1.0g/L substratum as the sole carbon source and the energy, and add N, P, Mg, Ca, Fe nutritive element.
10. the method for cultivation of biological cleaning strains in low-concentration volatile mixed organic waste gas as claimed in claim 9 is characterized in that: after cultivating the end of domestication initial stage, reduce the starch consumption in the substratum gradually, increase exhausted air quantity; Every 48 hours, stop air inlet and vibration, staticly settled 2-3 hour, get rid of supernatant liquor, and add the inoculum substratum.
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Cited By (9)

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CN103055670A (en) * 2013-01-11 2013-04-24 凯天环保科技股份有限公司 Method for managing volatile organic waste gas
CN104059864A (en) * 2014-05-26 2014-09-24 绍兴柯桥江滨水处理有限公司 Bacilluscereus as well as preparation method and application thereof
CN104826483A (en) * 2015-05-25 2015-08-12 永康市禄祥环保科技有限公司 Paint spraying waste gas and organic waste gas biological purification method
CN105771625A (en) * 2015-01-09 2016-07-20 现代自动车株式会社 Agent for removing malodor from painting booth, and method of removing malodor
CN106987517A (en) * 2017-05-02 2017-07-28 广东省微生物研究所（广东省微生物分析检测中心） A kind of VOCs degradation floras quickly tame the system and method for enrichment
CN108114593A (en) * 2018-01-18 2018-06-05 浙江大学 Using the method for absorption-degradation segmented biological reaction apparatus processing organic exhaust gas
CN110358721A (en) * 2019-06-19 2019-10-22 广州金鹏环保工程有限公司 A kind of the domestication system and method for degrading volatile organic compounds flora
CN110559840A (en) * 2019-09-19 2019-12-13 清远市益源环保科技有限公司 method and equipment for treating stink and VOCs (volatile organic compounds) through microorganisms
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CN103055670A (en) * 2013-01-11 2013-04-24 凯天环保科技股份有限公司 Method for managing volatile organic waste gas
CN103055670B (en) * 2013-01-11 2015-04-15 凯天环保科技股份有限公司 Method for managing volatile organic waste gas
CN104059864A (en) * 2014-05-26 2014-09-24 绍兴柯桥江滨水处理有限公司 Bacilluscereus as well as preparation method and application thereof
CN105771625A (en) * 2015-01-09 2016-07-20 现代自动车株式会社 Agent for removing malodor from painting booth, and method of removing malodor
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CN104826483A (en) * 2015-05-25 2015-08-12 永康市禄祥环保科技有限公司 Paint spraying waste gas and organic waste gas biological purification method
CN106987517A (en) * 2017-05-02 2017-07-28 广东省微生物研究所（广东省微生物分析检测中心） A kind of VOCs degradation floras quickly tame the system and method for enrichment
CN108114593A (en) * 2018-01-18 2018-06-05 浙江大学 Using the method for absorption-degradation segmented biological reaction apparatus processing organic exhaust gas
CN110358721A (en) * 2019-06-19 2019-10-22 广州金鹏环保工程有限公司 A kind of the domestication system and method for degrading volatile organic compounds flora
CN110559840A (en) * 2019-09-19 2019-12-13 清远市益源环保科技有限公司 method and equipment for treating stink and VOCs (volatile organic compounds) through microorganisms
CN115138350A (en) * 2022-06-27 2022-10-04 安徽师范大学 Method for biological regeneration of activated carbon for adsorbing gaseous toluene

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