CN101530456A - Method for extracting antioxidant active substance in clover - Google Patents
Method for extracting antioxidant active substance in clover Download PDFInfo
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- CN101530456A CN101530456A CN200910119136A CN200910119136A CN101530456A CN 101530456 A CN101530456 A CN 101530456A CN 200910119136 A CN200910119136 A CN 200910119136A CN 200910119136 A CN200910119136 A CN 200910119136A CN 101530456 A CN101530456 A CN 101530456A
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Abstract
The invention relates to a method for extracting antioxidant active substance in clover, which includes steps of ethanol extraction, polyamide purifying, eluant condensation and drying for obtaining the extract with strong antioxidant activity, and the method belongs to natural product extraction field. The invention adopts clover as raw material to prepare strong antioxidant active substance, has simple process, low cost and simple device, is suitable for large-scale production, and establishes base for further exploiting antioxidant function foodstuff. The prepared clover antioxidant active substance product is suitable for industries such as medicine, health care product, foodstuff antioxidant and cosmetic, and extract residue can be used as forage and fertilizer after processing, thereby realizing comprehensive utilization.
Description
Technical field
The invention belongs to field of natural product extraction, particularly a kind of method of from Herba Trifolii Pratentis, extracting anti-oxidation active substance.
Background technology
Herba Trifolii Pratentis is the pulse family Clover, draft; Be distributed in temperate zone and subtropical zone, be world-renowned feedstuff and cover plant, can be used for water and soil conservation, herbage, green manure simultaneously; Be rich in flavone compound; The herb hyoscine has the effect of heat clearing away, removing heat from blood.What China's introducing was cultivated has an appointment 7 kinds, and frequent species has butch clover (Trifolium repens Linn.), red Herba Trifolii Pratentis (Trifolium pratense Linn.) etc.
(free radical, FR) damage of pair cell and tissue is its morbific basis to free radical.FR oxidation cell membrane causes the cell membrane degeneration, makes cell can not absorb nutrition, drains refuse; FR attacks the DNA purine bases, causes gene mutation; The FR vasoactive makes its elasticity reduce, break easily, causes the generation of diseases such as hypertension; FR corrodes body tissue, but exciting human discharges various inflammatory factors, causes non-mushroom inflammation; FR corrodes brain cell, the disease of presenile dementia occurs; Lipoprotein causes the deposition of cholesterol to blood vessel wall in the FR oxidation blood, causes heart disease and stroke.In a word, FR can corrode body tissue, disturbs the important physical process, cause the body function disorder.
Herba Trifolii Pratentis is rich in flavone compound, and it is huge to originate, but the extraction and the antioxidation activity in vitro of its polyphenoils (ATR) still lacked research.At present, do not see Herba Trifolii Pratentis antioxidant Study on extraction report.
Summary of the invention
The purpose of this invention is to provide a kind of low cost, be suitable for method industrialization development, that from Herba Trifolii Pratentis, extract anti-oxidation active substance.
The present invention is achieved through the following technical solutions:
A kind of method of from Herba Trifolii Pratentis, extracting anti-oxidation active substance, the step of its extracting method is as follows:
(1) Herba Trifolii Pratentis is dried, pulverizes in the place, cool place, cross 10～50 mesh sieves, form the SANYE grass meal;
(2) adding concentration in the SANYE grass meal is that 60～95% alcoholic solution extracts, and extracts solid-liquid ratio 1:10～1:30 (w/v), and extraction time is 2～6h, extracts 60～85 ℃ of temperature, repeats lixiviate 2～4 times, and extracting solution concentrates the back and goes up polyamide column and carry out purification;
(3) the distilled water eluting of 6～10 times of bed volumes of employing is removed water-solubility impurities such as polysaccharide, albumen;
(4) the alcoholic solution eluting of 60～95% concentration of 3～8 times of bed volumes of employing, eluent obtains anti-oxidation active substance through concentrated, lyophilization.
And the polyamide that purification adopts is 50～400 orders, and last sample loading mode adopts and dynamically goes up sample loading mode.
Advantage of the present invention and beneficial effect are:
1. to adopt Herba Trifolii Pratentis be feedstock production strong anti-oxidation active substance in the present invention, and technology is simple, invests less and equipment simply is suitable for large-scale production, and be a step to be developed as anti-oxidation functional food to lay a good foundation.
2. prepared Herba Trifolii Pratentis anti-oxidation active substance product is applicable to industries such as medicine and health product, food antioxidant, cosmetics, and the extract residue can be used as feedstuff, fertilizer after processing, realizes comprehensive utilization.
The specific embodiment
The present invention is described in further detail by following examples, but the technology contents that present embodiment is narrated is illustrative, rather than determinate, should not limit to protection scope of the present invention with this.
The method step that extracts anti-oxidation active substance from Herba Trifolii Pratentis involved in the present invention is as follows:
(1) Herba Trifolii Pratentis is dried, pulverizes in the place, cool place, cross 40 mesh sieves, form the SANYE grass meal;
(2) take by weighing 100 gram SANYE grass meals, adding 1000mL concentration is 60% alcoholic solution in this SANYE grass meal, 70 ℃ of extraction times are 4h, repeat lixiviate 3 times, extracting solution merges and is concentrated into to be gone up polyamide column behind the 1000mL and carries out purification, polyamide can adopt 100～200 orders, adopts and dynamically goes up sample loading mode; With TLC (thin layer chromatography) monitoring stream fluid, stop to go up sample when beginning to leak;
(3) the distilled water eluting of 8 times of bed volumes of employing is fully to remove water-solubility impurities such as polysaccharide, albumen;
(4) employing 5 times of bed volumes, concentration are 95% alcoholic solution eluting, and the eluent after the merging obtains anti-oxidation active substance through concentrated, lyophilization.
This antioxidation powder-product is mixed with 100ug/mL solution with methanol,, does test of DPPH free radical scavenging and the test of FRAP method oxidation resistance with Vc (vitamin c) and the positive contrast of BHT (2, the 4-di-tertiary butyl methyl phenol) with concentration.The results are shown in Table 1 and table 2 shown in.
Table 1 100ug/mL sample DPPH free radical scavenging activity
*Represent to exist between each sample significant difference (P＜0.01)
Table 2 100ug/mL sample F RAP method oxidation resistance
*Represent to exist between each sample significant difference (P＜0.01)
Claims (2)
1. method of from Herba Trifolii Pratentis, extracting anti-oxidation active substance, it is characterized in that: the step of its extracting method is as follows:
(1) Herba Trifolii Pratentis is dried, pulverizes in the place, cool place, cross 10～50 mesh sieves, form the SANYE grass meal;
(2) adding concentration in the SANYE grass meal is that 60～95% alcoholic solution extracts, and extracts solid-liquid ratio 1:10～1:30 (w/v), and extraction time is 2～6h, extracts 60～85 ℃ of temperature, repeats lixiviate 2～4 times, and extracting solution concentrates the back and goes up polyamide column and carry out purification;
(3) the distilled water eluting of 6～10 times of bed volumes of employing is removed water-solubility impurities such as polysaccharide, albumen;
(4) the alcoholic solution eluting of 60～95% concentration of 3～8 times of bed volumes of employing, eluent obtains anti-oxidation active substance through concentrated, lyophilization.
2. a kind of method of from Herba Trifolii Pratentis, extracting anti-oxidation active substance according to claim 1, it is characterized in that: the polyamide that purification adopts is 50～400 orders, last sample loading mode adopts and dynamically goes up sample.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200910119136A CN101530456A (en) | 2009-03-04 | 2009-03-04 | Method for extracting antioxidant active substance in clover |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910119136A CN101530456A (en) | 2009-03-04 | 2009-03-04 | Method for extracting antioxidant active substance in clover |
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| CN101530456A true CN101530456A (en) | 2009-09-16 |
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| CN200910119136A Pending CN101530456A (en) | 2009-03-04 | 2009-03-04 | Method for extracting antioxidant active substance in clover |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107753389A (en) * | 2016-11-30 | 2018-03-06 | 江苏创铭医疗器械有限公司 | A kind of recombined collagen foaming pectin facial mask and preparation method thereof |
| CN112972321A (en) * | 2019-12-16 | 2021-06-18 | 兰州大学 | Natural antioxidant for cosmetics and preparation method thereof |
-
2009
- 2009-03-04 CN CN200910119136A patent/CN101530456A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107753389A (en) * | 2016-11-30 | 2018-03-06 | 江苏创铭医疗器械有限公司 | A kind of recombined collagen foaming pectin facial mask and preparation method thereof |
| CN112972321A (en) * | 2019-12-16 | 2021-06-18 | 兰州大学 | Natural antioxidant for cosmetics and preparation method thereof |
| KR20210077593A (en) * | 2019-12-16 | 2021-06-25 | 란저우 유니버시티 | Natural antioxidants for use in cosmetics and manufacturing method thereof |
| KR102511379B1 (en) | 2019-12-16 | 2023-03-16 | 란저우 유니버시티 | Natural antioxidants for use in cosmetics and manufacturing method thereof |
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Open date: 20090916 |