CN101502326B - Method for preparing vitamin E nano liposome - Google Patents
Method for preparing vitamin E nano liposome Download PDFInfo
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- CN101502326B CN101502326B CN2009100251935A CN200910025193A CN101502326B CN 101502326 B CN101502326 B CN 101502326B CN 2009100251935 A CN2009100251935 A CN 2009100251935A CN 200910025193 A CN200910025193 A CN 200910025193A CN 101502326 B CN101502326 B CN 101502326B
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Abstract
The invention provides a preparation method of a vitamin E nano liposome, belonging to the functional health food technology field. The vitamin E is used as core material and lecithin, cholesterol and Tween 80 are used as wall material and vitamin E common liposome is prepared using an ethanol injection method and then subjected to ultra-high pressure homogeneity, thus transparent or semi-transparent vitamin E nano liposome with average diameter of les than 100nm and encapsulation rate of higher than 90% is obtained. The preparation method is suitable for continuous production and solves problem of saled vitamin E in aspects of stability, water dispersibility and oral absorbency and is widely applied on preparation method of functional water based food.
Description
Technical field
A kind of preparation method of vitamin E nano liposome belongs to the functional health-care food technical field.
Background technology
Vitamin E is fat-soluble natural, suppresses lipid peroxidation through the active of passivation peroxylradicals or with the lipid peroxidation radical reaction, helps to improve immunity and disease preventing and treating, influences gene transcription and expression.That vitamin E also has is anti-ageing, improve immunity of organisms, the effect of the disorderly various diseases that causes of prevention Radical Metabolism and prevention presenile senile dementia, antitumor, the aspects such as radiation injury that prevent DNA, is the focus that people study always.At present, start one natural VE heat abroad, consume 2500～3000 tons every year, and with annual 10% speed increment, the replenishers of become widely used disease-prevention health in American-European countries's mid-aged population, promoting longevity.But commercially available vitamin E exist such as poorly water-soluble, responsive to light and heat, be prone to oxidation, do not have the slowly-releasing effect, many problems such as bioavilability is low.
Many researchers have been attempted vitamin E is carried out microencapsulation embedding protection; The domestic hydrophily wall material of mostly selecting carries out embedding, but this type wall material can not provide effective slow releasing function, after the wall material is met water (like saliva); Promptly dissolved, thus a large amount of releases of core caused.And liposome is as a kind of authorized by state formulation, has been incorporated in 2005 editions and " in the Chinese pharmacopoeia, earned widespread respect and use.Present existing liposome preparation technology is a lot of, and common has tens kinds, and these methods respectively have characteristics, generally selects according to the character and the experiment purpose of core.The technology of preparing of nano liposomes also has some special requirements, often needs to adopt certain means such as ultrasonic, homogeneous or extruding etc. that the liposome of conventional size is crushed to suitable dimensions.Film-ultrasonic method is prepared in laboratory nano liposomes a kind of method the most commonly used, but amplifies difficulty.Ethanol injects and combines homogeneous (or extruding) technology is the method that is suitable for large-scale production; Not only can avoid the use of toxic solvent (like chloroform, methyl alcohol) and other too controversial raw material; And can control the particle diameter of liposome, simple and easy to do, favorable reproducibility.
Adopt nano liposomes embedding vitamin E can strengthen the stability of vitamin E; Prevent oxidative degradation; And can improve that it is water-soluble, improve bioavailability, thereby promote absorption of human body; Can it be made an addition to aqueous food as nutrition fortifier, also will have broad application prospects in field of health care food.
Summary of the invention
The purpose of this invention is to provide the method that a kind of ethanol injection-super-high-pressure homogenization prepares vitamin E liposome; The protection and the slowly-releasing of vitamin E have been realized; Improved the water-soluble of vitamin E; Improve its bioavilability, can realize safe, the serialization production of vitamin E nano liposome simultaneously.
Technical scheme of the present invention: a kind of preparation method of vitamin E nano liposome; With the vitamin E is core, and lecithin, cholesterol and Tween 80 are the wall material, realizes the nano liposomesization of vitamin E through alcohol injection and super-high-pressure homogenization; Be applicable to serialization production, preparation technology is:
(1) adopt alcohol injection to prepare the conventional liposome of vitamin E: main wall material adopts lecithin; The supplementary wall material is cholesterol and Tween 80; Consumption is a vitamin E: lecithin: cholesterol: the mass ratio of Tween 80 is 4～20: 25: 4: 18, it was 0.1-0.2g/mL that the adding absolute ethyl alcohol makes the concentration of lecithin; In 55 ℃ of water-baths to add material dissolution; Be injected into fast in doubly aquation medium pH 7.4 of absolute ethyl alcohol volume 10-15, the 0.01mol/L phosphate buffer with syringe while stirring; Stir aquation 30min, rotary evaporation is removed ethanol (55 ℃, vacuum 0.1MPa); Cooling promptly gets vitamin E liposome rapidly;
Said lecithin is selected a kind of in egg yolk lecithin or the soybean lecithin for use;
(2) adopt the super-high-pressure homogenization method with the vitamin E liposome nanometerization: the vitamin E liposome that makes is handled through super-high-pressure homogenization, and pressure 20000～25000PSI circulates 1～3 time, promptly gets vitamin E nano liposome.
The mensuration of particle diameter: adopt the dynamic light scattering particle size appearance to measure, measure 25 ℃ of temperature.
The mensuration of envelop rate: adopt high-performance liquid chromatogram determination.
Useful achievement of the present invention: with the vitamin E is core, and lecithin, cholesterol are the film material, are aided with Tween 80; Ethanol injection-super-high-pressure homogenization method obtains outward appearance and is transparent or semitransparent, average grain diameter less than 100nm; Envelop rate is higher than 90% vitamin E nano liposome, has realized the protection and the slowly-releasing of vitamin E, has improved the water-soluble of vitamin E; Improve its bioavilability, can realize safe, the serialization production of vitamin E nano liposome simultaneously.
Description of drawings
Fig. 1 vitamin E nano liposome particle diameter distributes.
The specific embodiment
Accurately take by weighing vitamin E 0.06g, egg yolk lecithin 0.25g, cholesterol 0.04g and Tween 80 0.18g, add the 2mL absolute ethyl alcohol and extremely dissolve, be injected into 20mLpH 7.4 fast with syringe while stirring in 55 ℃ of water-baths; 0.01mol/L in the phosphate buffer, stir aquation 30min, rotary evaporation is removed (55 ℃ of ethanol; Vacuum 0.1MPa), cooling rapidly, super-high-pressure homogenization (25000PSI; Circulate 1 time), obtain vitamin E nano liposome, after charge into N
2Sealing places refrigerator cold-storage to preserve.Recording average grain diameter is 81.25nm, and polydispersity index is 0.164, and envelop rate is 92.7%.
Embodiment 2
Accurately take by weighing vitamin E 0.36g, soybean lecithin 0.5g, cholesterol 0.08g and Tween 80 0.36g, add the 3mL absolute ethyl alcohol and extremely dissolve, be injected into 40mLpH 7.4 fast with syringe while stirring in 55 ℃ of water-baths; 0.01mol/L in the phosphate buffer, stir aquation 30min, rotary evaporation is removed (55 ℃ of ethanol; Vacuum 0.1MPa), cooling rapidly, high-pressure homogeneous (20000PSI; Circulate 3 times), obtain vitamin E nano liposome, after charge into N
2Sealing places refrigerator cold-storage to preserve.Recording average grain diameter is 88.07nm, and polydispersity index is 0.114, and envelop rate is 92.8%.
Claims (1)
1. the preparation method of a vitamin E nano liposome; It is characterized in that with the vitamin E being core, lecithin, cholesterol and Tween 80 are the wall material, realize the nano liposomesization of vitamin E through alcohol injection and super-high-pressure homogenization; Be applicable to serialization production, preparation technology is:
(1) adopt alcohol injection to prepare the conventional liposome of vitamin E: main wall material adopts lecithin; The supplementary wall material is cholesterol and Tween 80; Consumption is a vitamin E: lecithin: cholesterol: the mass ratio of Tween 80 is 4～20: 25: 4: 18, it was 0.1-0.2g/mL that the adding absolute ethyl alcohol makes the concentration of lecithin; In 55 ℃ of water-baths to add material dissolution; Be injected into fast in doubly aquation medium pH 7.4 of absolute ethyl alcohol volume 10-15, the 0.01mol/L phosphate buffer with syringe while stirring, stir aquation 30min, rotary evaporation is removed ethanol; Cooling promptly gets vitamin E liposome rapidly;
Said lecithin is selected a kind of in egg yolk lecithin or the soybean lecithin for use;
(2) adopt the super-high-pressure homogenization method with the vitamin E liposome nanometerization: the vitamin E liposome that makes is handled through super-high-pressure homogenization, and pressure 20000～25000PSI circulates 1～3 time, promptly gets vitamin E nano liposome.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2009100251935A CN101502326B (en) | 2009-02-23 | 2009-02-23 | Method for preparing vitamin E nano liposome |
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| CN2009100251935A CN101502326B (en) | 2009-02-23 | 2009-02-23 | Method for preparing vitamin E nano liposome |
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| CN101502326A CN101502326A (en) | 2009-08-12 |
| CN101502326B true CN101502326B (en) | 2012-06-27 |
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Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101732251B (en) * | 2009-12-25 | 2012-09-26 | 海南美兰史克制药有限公司 | Oxiracetam liposome injection |
| CN101780041A (en) * | 2010-03-23 | 2010-07-21 | 南昌大学 | Polymer coated vitamin E liposome and preparation method thereof |
| CN101803741B (en) * | 2010-04-20 | 2012-05-02 | 浙江新和成股份有限公司 | Method for preparing nano vitamin E water dispersing powder preparation by using supergravity method |
| CN101822320B (en) * | 2010-05-22 | 2013-07-03 | 鼎正动物药业（天津）有限公司 | Vitamin water aqua and preparation method thereof |
| CN106031711A (en) * | 2015-03-18 | 2016-10-19 | 邓学峰 | Preparation method and applications of ilaprazole sodium liposome |
| CN106031733A (en) * | 2015-03-18 | 2016-10-19 | 邓学峰 | Preparation method and applications of rabeprazole sodium liposome |
| CN104785178A (en) * | 2015-03-26 | 2015-07-22 | 江南大学 | Thermosensitive liposome as well as preparation method and application thereof |
| CN105287405A (en) * | 2015-10-30 | 2016-02-03 | 新疆医科大学第一附属医院 | Albendazole nano-liposomes freeze-dried powder and preparation method thereof |
| CN107252118A (en) * | 2017-06-29 | 2017-10-17 | 安徽康博特保健食品有限公司 | A kind of preparation method of sugar-free vitamin C lozenges |
| CN107996834A (en) * | 2017-11-30 | 2018-05-08 | 味氏（广东）生物科技股份有限公司 | Feed addictive, its preparation method and include its feed |
| CN108576779B (en) * | 2018-02-11 | 2021-11-12 | 湖北工业大学 | Konjac glucomannan-liposome composite nano food delivery system and preparation method and application thereof |
| CN109329927A (en) * | 2018-08-17 | 2019-02-15 | 刘翠兰 | A kind of nanometer grade liposome encapsulating ubiquinone10Preparation and its sterile large-scale preparation method |
| CN109589278A (en) * | 2018-10-10 | 2019-04-09 | 昆明贝泰妮生物科技有限公司 | The preparation process of high stable prinsepia utilis royle oil liposome and its application in cosmetics |
| CN109480215A (en) * | 2018-11-05 | 2019-03-19 | 浙江工业大学 | A kind of surimi product and preparation method thereof |
| CN109463751B (en) * | 2018-12-25 | 2022-02-08 | 江苏艾兰得营养品有限公司 | Preparation method of coenzyme Q10 liposome health food |
| CN110679953A (en) * | 2019-10-23 | 2020-01-14 | 吉林大学 | Preparation method of nano liposome embedded with egg white source active peptide |
| CN110835587A (en) * | 2019-11-20 | 2020-02-25 | 茗汲(浙江)生物科技有限公司 | White tea antibacterial handmade soap and preparation method thereof |
| CN114847488A (en) * | 2022-05-17 | 2022-08-05 | 江南大学 | Preparation method of orange-flavored vitamin A-vitamin D nano-composite carrier with high stability and bioavailability |
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| CN1546016A (en) * | 2003-12-04 | 2004-11-17 | 东南大学 | Nanometer preparation of natural vitamin E and its preparation |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1546016A (en) * | 2003-12-04 | 2004-11-17 | 东南大学 | Nanometer preparation of natural vitamin E and its preparation |
| CN101214219A (en) * | 2007-12-28 | 2008-07-09 | 江南大学 | Method for preparing vitamin A and vitamin E nano-sphere/microsphere double-embedding system |
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| Title |
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