CN109329927A - A kind of nanometer grade liposome encapsulating ubiquinone10Preparation and its sterile large-scale preparation method - Google Patents
A kind of nanometer grade liposome encapsulating ubiquinone10Preparation and its sterile large-scale preparation method Download PDFInfo
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- CN109329927A CN109329927A CN201810939311.2A CN201810939311A CN109329927A CN 109329927 A CN109329927 A CN 109329927A CN 201810939311 A CN201810939311 A CN 201810939311A CN 109329927 A CN109329927 A CN 109329927A
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- ubiquinone
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- 238000002360 preparation method Methods 0.000 title claims abstract description 66
- 239000002502 liposome Substances 0.000 title claims abstract description 51
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 62
- 235000019441 ethanol Nutrition 0.000 claims abstract description 34
- 238000001704 evaporation Methods 0.000 claims abstract description 22
- ACTIUHUUMQJHFO-UPTCCGCDSA-N coenzyme Q10 Chemical compound COC1=C(OC)C(=O)C(C\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CC\C=C(/C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UPTCCGCDSA-N 0.000 claims abstract description 21
- GVJHHUAWPYXKBD-UHFFFAOYSA-N (±)-α-Tocopherol Chemical compound OC1=C(C)C(C)=C2OC(CCCC(C)CCCC(C)CCCC(C)C)(C)CCC2=C1C GVJHHUAWPYXKBD-UHFFFAOYSA-N 0.000 claims abstract description 18
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims abstract description 16
- 230000008020 evaporation Effects 0.000 claims abstract description 14
- 238000000034 method Methods 0.000 claims abstract description 14
- JLPULHDHAOZNQI-ZTIMHPMXSA-N 1-hexadecanoyl-2-(9Z,12Z-octadecadienoyl)-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCCCCCC\C=C/C\C=C/CCCCC JLPULHDHAOZNQI-ZTIMHPMXSA-N 0.000 claims abstract description 9
- 229930003427 Vitamin E Natural products 0.000 claims abstract description 9
- WIGCFUFOHFEKBI-UHFFFAOYSA-N gamma-tocopherol Natural products CC(C)CCCC(C)CCCC(C)CCCC1CCC2C(C)C(O)C(C)C(C)C2O1 WIGCFUFOHFEKBI-UHFFFAOYSA-N 0.000 claims abstract description 9
- 229940083466 soybean lecithin Drugs 0.000 claims abstract description 9
- 235000019165 vitamin E Nutrition 0.000 claims abstract description 9
- 229940046009 vitamin E Drugs 0.000 claims abstract description 9
- 239000011709 vitamin E Substances 0.000 claims abstract description 9
- 235000012000 cholesterol Nutrition 0.000 claims abstract description 8
- ACTIUHUUMQJHFO-UHFFFAOYSA-N Coenzym Q10 Natural products COC1=C(OC)C(=O)C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)=C(C)C1=O ACTIUHUUMQJHFO-UHFFFAOYSA-N 0.000 claims description 18
- 235000017471 coenzyme Q10 Nutrition 0.000 claims description 18
- NPCOQXAVBJJZBQ-UHFFFAOYSA-N reduced coenzyme Q9 Natural products COC1=C(O)C(C)=C(CC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)CCC=C(C)C)C(O)=C1OC NPCOQXAVBJJZBQ-UHFFFAOYSA-N 0.000 claims description 16
- 229940035936 ubiquinone Drugs 0.000 claims description 16
- 239000000203 mixture Substances 0.000 claims description 15
- 239000007788 liquid Substances 0.000 claims description 14
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 claims description 11
- 229910000403 monosodium phosphate Inorganic materials 0.000 claims description 11
- 235000019799 monosodium phosphate Nutrition 0.000 claims description 11
- AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000002390 rotary evaporation Methods 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 abstract description 8
- 230000008901 benefit Effects 0.000 abstract description 6
- 230000003647 oxidation Effects 0.000 abstract description 6
- 238000007254 oxidation reaction Methods 0.000 abstract description 6
- 238000005538 encapsulation Methods 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 5
- 239000002537 cosmetic Substances 0.000 abstract description 3
- 235000013305 food Nutrition 0.000 abstract description 3
- 230000008569 process Effects 0.000 abstract description 3
- 229940107161 cholesterol Drugs 0.000 abstract 1
- 239000003814 drug Substances 0.000 description 6
- 229940079593 drug Drugs 0.000 description 5
- 239000005515 coenzyme Substances 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 230000007812 deficiency Effects 0.000 description 2
- 206010054949 Metaplasia Diseases 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000000232 gallbladder Anatomy 0.000 description 1
- 230000015689 metaplastic ossification Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 150000003904 phospholipids Chemical class 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 230000009467 reduction Effects 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000008685 targeting Effects 0.000 description 1
- 230000010148 water-pollination Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/15—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23P—SHAPING OR WORKING OF FOODSTUFFS, NOT FULLY COVERED BY A SINGLE OTHER SUBCLASS
- A23P10/00—Shaping or working of foodstuffs characterised by the products
- A23P10/30—Encapsulation of particles, e.g. foodstuff additives
- A23P10/35—Encapsulation of particles, e.g. foodstuff additives with oils, lipids, monoglycerides or diglycerides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/02—Cosmetics or similar toiletry preparations characterised by special physical form
- A61K8/14—Liposomes; Vesicles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/33—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds containing oxygen
- A61K8/35—Ketones, e.g. benzophenone
- A61K8/355—Quinones
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/55—Phosphorus compounds
- A61K8/553—Phospholipids, e.g. lecithin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/63—Steroids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/67—Vitamins
- A61K8/678—Tocopherol, i.e. vitamin E
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
Abstract
The present invention relates to food, cosmetics and preparation technique fields, and in particular to a kind of nanometer grade liposome encapsulating ubiquinone10Preparation and its sterile large-scale preparation method.The nanometer grade liposome encapsulates ubiquinone10Preparation, due to by soybean lecithin, cholesterol, vitamin E, ubiquinone10, ethyl alcohol and buffer be made, and define that the pH value of buffer is 6.0~7.0 so that the nanometer grade liposome encapsulates ubiquinone10Preparation can be avoided preparation leakage and lipid oxidation, and have the advantages that encapsulation rate is high.The nanometer grade liposome encapsulates ubiquinone10Preparation sterile large-scale preparation method, due to by multiple homogeneous and repeatedly evaporation, and combine and buffer substep be added, and then obtained nanometer grade liposome is made to encapsulate ubiquinone10Preparation can be avoided preparation leakage and lipid oxidation, and have the advantages that encapsulation rate is high, and the preparation method has the characteristics that simple process, production cost are low.
Description
Technical field
The present invention relates to food, cosmetics and preparation technique fields, and in particular to a kind of nanometer grade liposome encapsulating coenzyme
Q10Preparation and its sterile large-scale preparation method.
Background technique
Liposome is the hydrophily vesica being made of phospholipid bilayer, has and improves the stability for being encapsulated drug, promotees
Into Drug Percutaneous Absorption, extend drug treating time and targeting and reduction poisonous side effect of medicine to local diseased region
The features such as.Therefore, liposome is widely used to food, cosmetics and drug as carrier.
Liposome and carrier lipid body application prospect are very good at present, but the production technology of liposome is answered in the prior art
Miscellaneous and expensive, unreasonable formula and technique easily causes the fused of liposome and causes drug leakage, lipid oxidation, encapsulation rate
Low defect, and complicated technique will also result in the defect of operating difficulties, and drawbacks described above is that current restriction liposome is extensive
The primary bottleneck that metaplasia produces, and the difficulty that enterprise is faced at present.
Summary of the invention
It is an object of the present invention in view of the deficiencies of the prior art, provide a kind of nanometer grade liposome encapsulating coenzyme
The preparation of the preparation of Q10, nanometer grade liposome encapsulating Co-Q10 can be avoided preparation leakage and lipid oxidation, and have packet
The high advantage of envelope rate.
The second object of the present invention is in view of the deficiencies of the prior art, to provide a kind of nanometer grade liposome encapsulating coenzyme
The sterile large-scale preparation method of the preparation of Q10, which has simple process, production cost low, and is avoided that preparation
The advantages of leakage and lipid oxidation.
One of to achieve the goals above, the present invention adopts the following technical scheme:
A kind of nanometer grade liposome encapsulating ubiquinone is provided10Preparation, it is made of the component of following weight percent:
Surplus is ethyl alcohol；
The buffer is made of the component of following parts by weight:
5 parts~10 parts of disodium hydrogen phosphate
1.5 parts~5.5 parts of sodium dihydrogen phosphate
900 parts~1100 parts of water；
Wherein, the pH value of the buffer is 6.5~7.0.
Preferably, a kind of nanometer grade liposome encapsulates ubiquinone10Preparation, it by following weight percent group
Part is made:
Surplus is ethyl alcohol；
The buffer is made of the component of following parts by weight:
6 parts~10 parts of disodium hydrogen phosphate
1.5 parts~5 parts of sodium dihydrogen phosphate
950 parts~1050 parts of water；
Wherein, the pH value of the buffer is 6.6~7.0.
More preferably, a kind of nanometer grade liposome encapsulates ubiquinone10Preparation, it is by following weight percent
Component be made:
Surplus is ethyl alcohol；
The buffer is made of the component of following parts by weight:
7.16 parts of disodium hydrogen phosphate
3.3 parts of sodium dihydrogen phosphate
1000 parts of water；
Wherein, the pH value of the buffer is 6.8~7.0.
To achieve the goals above two, the present invention adopts the following technical scheme:
A kind of nanometer grade liposome encapsulating ubiquinone is provided10Preparation sterile large-scale preparation method, it includes following step
It is rapid:
Step 1: mixing and evaporating: by the soybean lecithin of formula ratio, cholesterol, vitamin E, ubiquinone10It is set with ethyl alcohol
It in container and is uniformly mixed, then under certain temperature, and carries out rotary evaporation ethyl alcohol under certain revolving speed, it is dry to obtain evaporation
The phosphatide ubiquinone of film10；
Step 2: buffer and homogeneous is added: the phosphatide ubiquinone for the evaporation dry film that step 1 is obtained10With in formula one
Partial buffer mixes, and then carries out homogeneous, obtains the first homogeneous object；
Step 3: second evaporates and buffer is added: being evaporated second again to the first homogeneous object that step 2 obtains
Then alcohol is added remaining buffer in formula ratio, then be stirred, obtains mixture until liquid level is still；
Step 4: second of homogeneous: the mixture that step 3 is obtained carries out homogeneous, obtains the second homogeneous object；
Step 5: third time is evaporated: the second homogeneous object that step 4 is obtained is evaporated still to liquid level, that is, makes
Obtain the preparation of the nanometer grade liposome encapsulating Co-Q10.
In above-mentioned technical proposal, in the step 1, at 30 DEG C~40 DEG C, and in 40rpm/min~60rpm/min's
Rotary evaporation ethyl alcohol 1.5h~3h is carried out under revolving speed.
In above-mentioned technical proposal, in the step 2, the homogenizing time is 2min~5min, and the revolving speed of the homogeneous is
10000rpm/min~18000rpm/min.
In above-mentioned technical proposal, in the step 3, the time of the stirring is 2h~4h.
In above-mentioned technical proposal, the remaining buffer of the buffer of a part of the step 2 and the step 3
Volume ratio is 1~3:5~30.
In above-mentioned technical proposal, in the step 4, the time of the homogeneous is 10min~20min, and the homogeneous turns
Speed is 10000rpm/min~18000rpm/min.
Compared with prior art, beneficial effect is the present invention:
(1) a kind of nanometer grade liposome provided by the invention encapsulates ubiquinone10Preparation, due to solid by soybean lecithin, gallbladder
Alcohol, vitamin E, ubiquinone10, ethyl alcohol and buffer be made, and define that the pH value of buffer is 6.5~7.0 so that should
Nanometer grade liposome encapsulates ubiquinone10Preparation can be avoided preparation leakage and lipid oxidation, and have the advantages that encapsulation rate is high.
(2) a kind of nanometer grade liposome provided by the invention encapsulates ubiquinone10Preparation sterile large-scale preparation method,
It include: Step 1: mixing and evaporating；Step 2: buffer and homogeneous is added；Step 3: second evaporates and is added buffer；
Step 4: second of homogeneous；Step 5: third time is evaporated.Due to being evaporated by multiple homogeneous with multiple, and combine buffer
Substep is added, so that obtained nanometer grade liposome encapsulates ubiquinone10Preparation can be avoided preparation leakage and lipid oxygen
Change, and have the advantages that encapsulation rate is high, and the preparation method has simple process, production cost low, and adapts to extensive
The characteristics of production.
Specific embodiment
In order to which the technical problems, technical solutions and beneficial effects solved by the present invention is more clearly understood, below in conjunction with
Embodiment, the present invention will be described in further detail.It should be appreciated that specific embodiment described herein is only used to explain
The present invention is not intended to limit the present invention.
Embodiment 1
A kind of nanometer grade liposome encapsulating ubiquinone10Preparation, it is made of the component of following weight percent:
Surplus is ethyl alcohol；
The buffer is made of the component of following parts by weight:
7.16 parts of disodium hydrogen phosphate
3.3 parts of sodium dihydrogen phosphate
1000 parts of water；
Wherein, the pH value of the buffer is 6.8~7.0.
A kind of above-mentioned nanometer grade liposome encapsulates ubiquinone10Preparation sterile large-scale preparation method, it includes following step
It is rapid:
Step 1: mixing and evaporating: by the soybean lecithin of formula ratio, cholesterol, vitamin E, ubiquinone10It is set with ethyl alcohol
It in container and is uniformly mixed, then at 35 DEG C, and carries out rotary evaporation ethyl alcohol 2h under the revolving speed of 50rpm/min, obtain
Evaporate the phosphatide ubiquinone of dry film10；
Step 2: buffer and homogeneous is added: the phosphatide ubiquinone for the evaporation dry film that step 1 is obtained10With in formula one
Partial buffer mixes, and then carries out homogeneous, obtains the first homogeneous object；In the present embodiment, homogenizing time 3min, homogeneous
Revolving speed is 13000rpm/min；
Step 3: second evaporates and buffer is added: being evaporated second again to the first homogeneous object that step 2 obtains
Then alcohol is added remaining buffer in formula ratio, then be stirred 3h, obtains mixture until liquid level is still；
Step 4: second of homogeneous: the mixture that step 3 is obtained carries out homogeneous, obtains the second homogeneous object；This implementation
In example, the time of homogeneous is 15min, and the revolving speed of homogeneous is 14000rpm/min；
Step 5: third time is evaporated: the second homogeneous object that step 4 is obtained is evaporated still to liquid level, that is, makes
It obtains the nanometer grade liposome and encapsulates ubiquinone10Preparation.
In the present embodiment, the volume ratio of the remaining buffer of the buffer and step 3 of a part of step 2 is 2:
20。
Embodiment 2
A kind of nanometer grade liposome encapsulating ubiquinone10Preparation, it is made of the component of following weight percent:
Surplus is ethyl alcohol；
The buffer is made of the component of following parts by weight:
5 parts of disodium hydrogen phosphate
1.5 parts of parts of sodium dihydrogen phosphate
900 parts of water；
Wherein, the pH value of the buffer is 6.5~6.8.
A kind of above-mentioned nanometer grade liposome encapsulates ubiquinone10Preparation sterile large-scale preparation method, it includes following step
It is rapid:
Step 1: mixing and evaporating: by the soybean lecithin of formula ratio, cholesterol, vitamin E, ubiquinone10It is set with ethyl alcohol
It in container and is uniformly mixed, then at 30 DEG C, and carries out rotary evaporation ethyl alcohol 1.5h under the revolving speed of 60rpm/min, obtain
To the phosphatide ubiquinone of evaporation dry film10；
Step 2: buffer and homogeneous is added: the phosphatide ubiquinone for the evaporation dry film that step 1 is obtained10With in formula one
Partial buffer mixes, and then carries out homogeneous, obtains the first homogeneous object；In the present embodiment, homogenizing time 2min, homogeneous
Revolving speed is 10000rpm/min；
Step 3: second evaporates and buffer is added: being evaporated second again to the first homogeneous object that step 2 obtains
Then alcohol is added remaining buffer in formula ratio, then be stirred 2h, obtains mixture until liquid level is still；
Step 4: second of homogeneous: the mixture that step 3 is obtained carries out homogeneous, obtains the second homogeneous object；This implementation
In example, the time of homogeneous is 10min, and the revolving speed of homogeneous is 10000rpm/min；
Step 5: third time is evaporated: the second homogeneous object that step 4 is obtained is evaporated still to liquid level, that is, makes
It obtains the nanometer grade liposome and encapsulates ubiquinone10Preparation.
In the present embodiment, the volume ratio of the remaining buffer of the buffer and step 3 of a part of step 2 is 1:5.
Embodiment 3
A kind of nanometer grade liposome encapsulating ubiquinone10Preparation, it is made of the component of following weight percent:
Surplus is ethyl alcohol；
The buffer is made of the component of following parts by weight:
10 parts of disodium hydrogen phosphate
5.5 parts of sodium dihydrogen phosphate
1100 parts of water；
Wherein, the pH value of the buffer is 6.6~6.8.
A kind of above-mentioned nanometer grade liposome encapsulates ubiquinone10Preparation sterile large-scale preparation method, it includes following step
It is rapid:
Step 1: mixing and evaporating: by the soybean lecithin of formula ratio, cholesterol, vitamin E, ubiquinone10It is set with ethyl alcohol
It in container and is uniformly mixed, then at 40 DEG C, and carries out rotary evaporation ethyl alcohol 3h under the revolving speed of 40rpm/min, obtain
Evaporate the phosphatide ubiquinone of dry film10；
Step 2: buffer and homogeneous is added: the phosphatide ubiquinone for the evaporation dry film that step 1 is obtained10With in formula one
Partial buffer mixes, and then carries out homogeneous, obtains the first homogeneous object；In the present embodiment, homogenizing time 5min, homogeneous
Revolving speed is 18000rpm/min；
Step 3: second evaporates and buffer is added: being evaporated second again to the first homogeneous object that step 2 obtains
Then alcohol is added remaining buffer in formula ratio, then be stirred 4h, obtains mixture until liquid level is still；
Step 4: second of homogeneous: the mixture that step 3 is obtained carries out homogeneous, obtains the second homogeneous object；This implementation
In example, the time of homogeneous is 20min, and the revolving speed of homogeneous is 18000rpm/min；
Step 5: third time is evaporated: the second homogeneous object that step 4 is obtained is evaporated still to liquid level, that is, makes
It obtains the nanometer grade liposome and encapsulates ubiquinone10Preparation.
In the present embodiment, the volume ratio of the remaining buffer of the buffer and step 3 of a part of step 2 is 3:
30。
Embodiment 4
A kind of nanometer grade liposome encapsulating ubiquinone10Preparation, it is made of the component of following weight percent:
Surplus is ethyl alcohol；
The buffer is made of the component of following parts by weight:
6 parts of disodium hydrogen phosphate
2.5 parts of sodium dihydrogen phosphate
950 parts of water；
Wherein, the pH value of the buffer is 6.5~6.7.
A kind of above-mentioned nanometer grade liposome encapsulates ubiquinone10Preparation sterile large-scale preparation method, it includes following step
It is rapid:
Step 1: mixing and evaporating: by the soybean lecithin of formula ratio, cholesterol, vitamin E, ubiquinone10It is set with ethyl alcohol
It in container and is uniformly mixed, then at 32 DEG C, and carries out rotary evaporation ethyl alcohol 1.8h under the revolving speed of 55rpm/min, obtain
To the phosphatide ubiquinone of evaporation dry film10；
Step 2: buffer and homogeneous is added: the phosphatide ubiquinone for the evaporation dry film that step 1 is obtained10With in formula one
Partial buffer mixes, and then carries out homogeneous, obtains the first homogeneous object；In the present embodiment, homogenizing time 4min, homogeneous
Revolving speed is 12000rpm/min；
Step 3: second evaporates and buffer is added: being evaporated second again to the first homogeneous object that step 2 obtains
Then alcohol is added remaining buffer in formula ratio, then be stirred 2h, obtains mixture until liquid level is still；
Step 4: second of homogeneous: the mixture that step 3 is obtained carries out homogeneous, obtains the second homogeneous object；This implementation
In example, the time of homogeneous is 12min, and the revolving speed of homogeneous is 13000rpm/min；
Step 5: third time is evaporated: the second homogeneous object that step 4 is obtained is evaporated still to liquid level, that is, makes
It obtains the nanometer grade liposome and encapsulates ubiquinone10Preparation.
In the present embodiment, the volume ratio of the remaining buffer of the buffer and step 3 of a part of step 2 is 2:
15。
Embodiment 5
A kind of nanometer grade liposome encapsulating ubiquinone10Preparation, it is made of the component of following weight percent:
Surplus is ethyl alcohol；
The buffer is made of the component of following parts by weight:
8 parts of disodium hydrogen phosphate
4.5 parts of sodium dihydrogen phosphate
1050 parts of water；
Wherein, the pH value of the buffer is 6.7~6.9.
A kind of above-mentioned nanometer grade liposome encapsulates ubiquinone10Preparation sterile large-scale preparation method, it includes following step
It is rapid:
Step 1: mixing and evaporating: by the soybean lecithin of formula ratio, cholesterol, vitamin E, ubiquinone10It is set with ethyl alcohol
It in container and is uniformly mixed, then at 38 DEG C, and carries out rotary evaporation ethyl alcohol 2.5h under the revolving speed of 45rpm/min, obtain
To the phosphatide ubiquinone of evaporation dry film10；
Step 2: buffer and homogeneous is added: the phosphatide ubiquinone for the evaporation dry film that step 1 is obtained10With in formula one
Partial buffer mixes, and then carries out homogeneous, obtains the first homogeneous object；In the present embodiment, homogenizing time 3min, homogeneous
Revolving speed is 17000rpm/min；
Step 3: second evaporates and buffer is added: being evaporated second again to the first homogeneous object that step 2 obtains
Then alcohol is added remaining buffer in formula ratio, then be stirred 4h, obtains mixture until liquid level is still；
Step 4: second of homogeneous: the mixture that step 3 is obtained carries out homogeneous, obtains the second homogeneous object；This implementation
In example, the time of homogeneous is 18min, and the revolving speed of homogeneous is 17000rpm/min；
Step 5: third time is evaporated: the second homogeneous object that step 4 is obtained is evaporated still to liquid level, that is, makes
It obtains the nanometer grade liposome and encapsulates ubiquinone10Preparation.
In the present embodiment, the volume ratio of the remaining buffer of the buffer and step 3 of a part of step 2 is 3:
25。
The above embodiments are merely illustrative of the technical solutions of the present invention, rather than limiting the scope of the invention, although
The present invention is explained in detail referring to preferred embodiment, those skilled in the art should understand that, it can be to this hair
Bright technical solution is modified or replaced equivalently, without departing from the spirit and scope of technical solution of the present invention.
Claims (9)
1. a kind of nanometer grade liposome encapsulates ubiquinone10Preparation, it is characterised in that: it by following weight percent component system
At:
The buffer is made of the component of following parts by weight:
5 parts~10 parts of disodium hydrogen phosphate
1.5 parts~5.5 parts of sodium dihydrogen phosphate
900 parts~1100 parts of water；
Wherein, the pH value of the buffer is 6.5~7.0.
2. a kind of nanometer grade liposome according to claim 1 encapsulates ubiquinone10Preparation, it is characterised in that: it is by following
The component of weight percent is made:
The buffer is made of the component of following parts by weight:
6 parts~10 parts of disodium hydrogen phosphate
1.5 parts~5 parts of sodium dihydrogen phosphate
950 parts~1050 parts of water；
Wherein, the pH value of the buffer is 6.6~7.0.
3. a kind of nanometer grade liposome according to claim 1 encapsulates ubiquinone10Preparation, it is characterised in that: it is by following
The component of weight percent is made:
The buffer is made of the component of following parts by weight:
7.16 parts of disodium hydrogen phosphate
3.3 parts of sodium dihydrogen phosphate
1000 parts of water；
Wherein, the pH value of the buffer is 6.8~7.0.
4. a kind of nanometer grade liposome encapsulating ubiquinone described in claims 1 to 3 any one10Preparation sterile scale
Preparation method, it is characterised in that: it the following steps are included:
Step 1: mixing and evaporating: by the soybean lecithin of formula ratio, cholesterol, vitamin E, ubiquinone10Appearance is placed in ethyl alcohol
It in device and is uniformly mixed, then under certain temperature, and carries out rotary evaporation ethyl alcohol under certain revolving speed, obtain evaporation dry film
Phosphatide ubiquinone10；
Step 2: buffer and homogeneous is added: the phosphatide ubiquinone for the evaporation dry film that step 1 is obtained10With a part in formula
Buffer mix, then carry out homogeneous, obtain the first homogeneous object；
Step 3: second evaporates and buffer is added: carrying out ethanol evaporation again to the first homogeneous object that step 2 obtains, directly
Until liquid level is still, remaining buffer in formula ratio is then added, then be stirred, obtains mixture；
Step 4: second of homogeneous: the mixture that step 3 is obtained carries out homogeneous, obtains the second homogeneous object；
Step 5: third time is evaporated: the second homogeneous object that step 4 is obtained is evaporated still to liquid level, obtains institute
State nanometer grade liposome encapsulating ubiquinone10Preparation.
5. a kind of nanometer grade liposome according to claim 4 encapsulates ubiquinone10Preparation sterile prepare with scale side
Method, it is characterised in that: in the step 1, at 30 DEG C~40 DEG C, and under the revolving speed of 40rpm/min~60rpm/min into
Row rotary evaporation ethyl alcohol 1.5h~3h.
6. a kind of nanometer grade liposome according to claim 4 encapsulates ubiquinone10Preparation sterile prepare with scale side
Method, it is characterised in that: in the step 2, the homogenizing time is 2min~5min, and the revolving speed of the homogeneous is 10000rpm/
Min~18000rpm/min.
7. a kind of nanometer grade liposome according to claim 4 encapsulates ubiquinone10Preparation sterile prepare with scale side
Method, it is characterised in that: in the step 3, the time of the stirring is 2h~4h.
8. a kind of nanometer grade liposome according to claim 4 encapsulates ubiquinone10Preparation sterile prepare with scale side
Method, it is characterised in that: the volume ratio of the buffer of a part of the step 2 and the remaining buffer of the step 3 is 1
~3:5~30.
9. a kind of nanometer grade liposome according to claim 4 encapsulates ubiquinone10Preparation sterile prepare with scale side
Method, it is characterised in that: in the step 4, the time of the homogeneous is 10min~20min, and the revolving speed of the homogeneous is
10000rpm/min~18000rpm/min.
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