CN101491669A

CN101491669A - Anti-anaemia fish-protein iron peptide capsule

Info

Publication number: CN101491669A
Application number: CNA2008100637508A
Authority: CN
Inventors: 邓尚贵; 裘耀东; 杨立业; 夏志国; 谢超
Original assignee: ZHEJIANG HAILISHENG BIOTECHNOLOGY CO Ltd; Zhejiang Ocean University ZJOU
Current assignee: ZHEJIANG HAILISHENG BIOTECHNOLOGY CO Ltd; Zhejiang Ocean University ZJOU
Priority date: 2008-08-04
Filing date: 2008-08-04
Publication date: 2009-07-29

Abstract

The invention discloses a fish protein iron-peptide capsule (tablet or granule) for preventing iron deficiency anemia, which is prepared by turning low-value marine fish and leftover protein into composite polypeptide through protein enzymatic hydrolysis, modifying the composite polypeptide with ferrous iron so as to obtain fish protein iron-peptide and mixing the fish protein iron-peptide with safflower seed oil, beeswax, phospholipid, starch according to a certain weight ratio. The invention discloses three formulations and one optimum formulation. The chemical nature of the capsule is a chelate product of peptide, amino acid and ferrous iron. The protein content of the capsule is more than or equal to 30 percent, and the total iron content of each capsule is 10+/-0.5 mg. The capsule is a novel functional food for preventing iron deficiency anemia.

Description

A kind of anti-anaemia fish-protein iron peptide capsule

Technical field

The present invention relates to a kind ofly be mixed and processed by fish-protein iron peptide and other material, be fit to baby, teenager, person in middle and old age, especially the women is used to prevent iron deficiency anemia, function of dominant field of food.

Background technology

As everyone knows, ferrum is the human body trace elements necessary, and body ferrum is stocked deficiency or food iron deficiency, all can cause the ferrum malnutrition, iron deficiency anemia occurs, and human body health is worked the mischief; There is 10-30% crowd's iron deficiency in the whole world, the women is because physiological reason will be higher than the male, cause the main cause of body iron deficiency to comprise too much, the Deficiency of Intake of ferrum forfeiture, body is in requirement increase in certain period (as the stage of development), malabsorption, running defective etc., the common functional mass that improves nutritional anemia has ferrous lactate, heme iron, ferrous sulfate and glucose ferrous etc. at present.These functional mass all have certain abnormal smells from the patient, and as ferrous lactate slightly foreign odor and sweet metallic taste, heme iron has special odor, and ferrous sulfate has astringent taste, and the ferrous slightly caramel of glucose abnormal smells from the patient not only uses inconvenience, also can bring the bad problem of mouthfeel; Simultaneously, they also have certain color, as ferrous lactate is green white, heme iron is mulberry or pitchy, ferrous sulfate is a canescence or cream-coloured, glucose is ferrous to be yellow-gray or oyster, tends to change the color of food self during as food additive, influences attractive in appearance and commercial value.Modern science proves, little peptide especially dipeptides and tripeptides can be absorbed by animal economy and enter blood circulation for the histiocyte utilization, and little Toplink promotes the absorption of Ca, Fe, elements such as Cu, Zn to be confirmed; Metallic elements such as little peptide and ferrum combine the form that the five-membered ring that forms or hexatomic ring chemical compound can little peptide and are absorbed by body; Thereby a kind of fish-protein iron peptide that from low value sea water fish and leftover protein, extracts, this fish-protein iron peptide is ferrous carrier with the protein hydrolysate peptide, form white and tasteless peptide and ferrous chelate, color and abnormal smells from the patient problem have been solved simultaneously, make its mouthfeel better, thereby the capsule of being formed with fish-protein iron peptide and other material, make ferrum be in reducing condition by the peptide carrier, can avoid the difficulty of the difficult absorption that factors such as ferrous oxidation or phytic acid cause, be a kind of ideal resistance to iron deficiency anemia goods, purpose of the present invention that Here it is.

Summary of the invention

For overcoming the deficiencies in the prior art, the present invention utilizes the albumen of leftover bits and pieces such as marine low-value Fish and processing back, skin, bone, internal organs to be raw material, after the protein enzyme hydrolysis, become complex polypeptide and obtain the fish-protein iron peptide finished product, be mixed and made into capsule or tablet or granule by certain weight ratio by fish-protein iron peptide and safflower oil, Cera Flava, phospholipid and soluble starch through ferrous modification.

Technical scheme of the present invention is:

Raw material---pre-treatment---combinative enzyme hydrolysis---plate-and-frame filtration---ferrous modification---spray drying---fish-protein iron peptide---preparation---molding---packing---finished product.

Realize that primary technology of the present invention is making of fish-protein iron peptide, fish-protein iron peptide is that the albumen of the leftover bits and pieces of head, skin, bone, internal organs after low value sea water fish and the processing thereof becomes complex polypeptide after the protein enzyme hydrolysis, and after ferrous modification, obtain fish-protein iron peptide, concrete operations are as follows:

(1) raw material: leftover bits and pieces such as fresh low value sea water fish or the head after the processing of aquatic products, bone, skin, bone are dirty;

(2) pre-treatment: raw material cleans the back and pulverizes with pulverizer, adds entry by 1 weight ratio than 2-5, i.e. the water mill of the raw material of 1 part of weight adding 2-5 part raw material weight becomes the meat slurry;

(3) combinative enzyme hydrolysis: adjusting meat slurry PH is 5-7, ratio in vigor 1 to 1 adds papain (Nanning Pang Bo biological product company limited, pressed powder, 400,000 IU) and food flavor enzyme (Nanning Pang Bo biological product company limited, liquid, 2,000,000 IU), the enzyme amount is controlled to be 600-2400IU, is under 40 ℃-60 ℃ the condition limited hydrolysis 2-4 hour in temperature.Hydrolyzed solution is heated to 90 ℃-100 ℃, and keeps killing in 15 minutes the vigor of enzyme;

(4) plate-and-frame filtration: adopt 300 keevil frame filters to filter enzymolysis solution, obtain hydrolyzed solution;

(5) ferrous modification: adjust the PH to 6-8 of hydrolyzed solution, under continuous stirring condition, slowly add 50 parts of hydrolyzed solutions of by weight/volume and add 1 part of solution of ferrous chloride that concentration is 1-5%, and keep killing in 15-60 minute the vigor of enzyme;

(6) spray drying: adopt spray dryer to make the fish-protein iron peptide finished product with (5).

The above-mentioned technology of extracting fish-protein iron peptide from low value sea water fish and leftover protein is together applied for national inventing patent with the application.

(7) preparation; molding and packing: with the fish-protein iron peptide is that major ingredient apolegamy, safflower oil, Cera Flava, phosphide, starch etc. are made tablet, granule or fill capsule for adjuvant after mixing; its prescription is: by weight percentage: fish-protein iron peptide 25-50%; safflower oil 0.01-0.2%; Cera Flava 0.01-0.2%; phosphide 0.01-0.2%,, soluble starch 49.4-74.97%.

The physicochemical characteristics of fish-protein iron peptide capsule:

(a) capsule is a white, red, green or brown;

(b) chemical nature is peptide and aminoacid and ferrous chelate manufactured goods;

(c) protein content 〉=30%, total iron content 10 ± 0.5mg/ capsule.

Leftover bits and pieces aboundresources after marine low-value Fish and the processing thereof, with low cost, its protein is complex polypeptide by the protein enzyme hydrolysis and obtains fish-protein iron peptide through ferrous modification, again through preparation, obtain fish-protein iron peptide capsule, it has opened up the low value fish and following porter expects proteic high-valued development approach, has remarkable resistance to iron deficiency anemia function, be better than anti-anemia agent such as ferrous lactate commonly used at present, ferrous sulfate, both solved food iron content deficiency, absorption and utilization for ferrum provides abundant guarantee again, is the functional food of the novel anti-ferrum anemia of a class.Have not yet to see the relevant report of relevant said products.

Specific implementation method

Embodiment one:

A kind of fish-protein iron peptide capsule (or tablet, granule) by weight percentage, is made up of following component in its capsule: fish-protein iron peptide 25%, safflower oil 0.01%, Cera Flava 0.01%, phosphide 0.01%, soluble starch 74.97%.

Embodiment two:

A kind of fish-protein iron peptide capsule (or tablet, granule) by weight percentage, is made up of following component in its capsule: fish-protein iron peptide 40%, safflower oil 0.1%, Cera Flava 0.1%, phosphide 0.1%, soluble starch 59.7%.

Embodiment three:

A kind of fish-protein iron peptide capsule (or tablet, granule) by weight percentage, is made up of following component in its capsule: fish-protein iron peptide 50%, safflower oil 0.2%, Cera Flava 0.2%, phosphide 0.2%, soluble starch 49.6%.

Most preferred embodiment:

A kind of fish-protein iron peptide capsule (or tablet, granule) by weight percentage, is made up of following component in its capsule: fish-protein iron peptide 35%, safflower oil 0.1%, Cera Flava 0.1%, phosphide 0.1%, soluble starch 64.7%.

Effect:

Experimental technique: select the female ablactation Wistar of body weight 50-60 gram secondary rat, after hanging down the iron base feedstuff and consuming empty 3 weeks, get blood through the tail vein, measure content of hemoglobin, press tail blood content of hemoglobin, animal be divided into the blank group at random, give low dose group (2.5mgFe/kg/d), middle dosage group (5mgFe/kg/d), high dose group (7.5mgFe/kg/d) fish-protein iron peptide capsule group and ferrous lactate matched group, experiment periods was 4 weeks, measured anemia indexs of correlation such as the free former protoporphyrin of animal hematoglobin and erythrocyte.

Table 1 fish-protein iron peptide capsule to the influence of rat hemoglobin (n=10, * ± s) (g/L)

As seen from Table 1, after the experiment, each dosage group of fish-protein iron peptide capsule and ferrous lactate positive controls content of hemoglobin all are significantly higher than low ferrum matched group (p＜0.05).All the be significantly increased effect of low ferrum matched group content of hemoglobin of fish-protein iron peptide capsule and ferrous lactate is described.

Table 2 fish-protein iron peptide capsule to the influence of the free former protoporphyrin content of rat erythrocyte ((n=10, * ± s) (ug/dl)

As seen from Table 2, before the experiment, each free former protoporphyrin content of erythrocyte of organizing rat does not have significant difference ((p＞0.05).After the experiment, the free former protoporphyrin content of the erythrocyte of each additional fish-protein iron peptide capsule all is lower than the ferrum matched group, compare with matched group with high dose fish-protein iron peptide capsule group wherein that there were significant differences (p＜0.05), do not have significant difference with the ferrous lactate positive controls.The same effect that the free former protoporphyrin content of remarkable reduction erythrocyte is arranged of prompting fish-protein iron peptide capsule with ferrous lactate.

According to the experimental result of table 1 and table 2, the deducibility fish-protein iron peptide capsule has the obvious effect that improves iron deficiency anemia.

Claims

1, a kind of anti-anaemia fish-protein iron peptide capsule (or tablet, granule) is made up of following composition:

By weight percentage: fish-protein iron peptide 25-50%, safflower oil 0.01-0.2%, Cera Flava 0.01--0.2%, phosphatidase 10 .01-0.2%, soluble starch 49.4-74.97%, mix and form.

2, the composition of anti-anaemia fish-protein iron peptide according to claim 1 (or tablet, granule) optimum formula is: by weight percentage: fish-protein iron peptide 35%, safflower oil 0.1%, Cera Flava 0.1%, phosphide 0.1%, soluble starch 64.7%, mix and form.