CN101491607A - Total triterpene compound extraction and separation method from apple and use thereof - Google Patents
Total triterpene compound extraction and separation method from apple and use thereof Download PDFInfo
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- CN101491607A CN101491607A CNA2009100212589A CN200910021258A CN101491607A CN 101491607 A CN101491607 A CN 101491607A CN A2009100212589 A CNA2009100212589 A CN A2009100212589A CN 200910021258 A CN200910021258 A CN 200910021258A CN 101491607 A CN101491607 A CN 101491607A
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Abstract
The invention relates to a method for extracting and separating total triterpenoid from an apple and application thereof. For a long time, the apples are used in large quantities for production of fresh foods and fruit juices, and for deep processing and extraction of active ingredients of the apples, the high-temperature boiling and solvent dipping are often adopted; and in markets, the apple polyphenol extracted from the apples by extremely few factories is only a crude extract, and the deep separating purification is not adopted. In the method of the invention, green apples and ripe apples or apple peel residues are fully washed, ground, placed in an ultrasonic device according to the liquid-solid ratio of between 8 and 10:1ml/g, subjected to ultrasonic extraction at a temperature of between 25 and 60 DEG C for 30 to 60 minutes, and then subjected to filtering, condensing, extraction and column chromatography to obtain the total triterpenoid. The method has the advantages of simple process, easy operation, easily obtained materials, and facilitates the preservation of the activity of the total triterpenoid of the apples. The separation and purification are carried out at the normal temperature and under the normal pressure, thereby saving energy; and the solvent is recycled, so that no pollution is caused.
Description
Technical field
The present invention relates to the extraction separation method and the application thereof of total triterpenes chemical compound in a kind of Fructus Mali pumilae.
Background technology
For a long time, Fructus Mali pumilae just is used to eat raw and fruit juice production in a large number, to its profound processing and extracts active ingredients adopt mostly high temperature steaming, solvent impregnated, if adopt its apparatus expensive of supercritical extraction and limitation arranged, though there is ultrasonic wave extraction to fail to be used for production in enormous quantities at present.
Several families of the only a few that occurs on the market extract apple polyphenol from Fructus Mali pumilae also be crude extract, fail deeper separation and purify, simple extract purification just with transport in eat raw, on beverage, health product and the cosmetics, at the bottom of the object technology content, the product emphasis is not outstanding, DeGrain fails really to bring into play the gold value of Fructus Mali pumilae industry.
Summary of the invention
The object of the present invention is to provide a kind of technology simple, raw material is easy to get, and helps keeping the extraction separation method and the application thereof of total triterpenes chemical compound in the active Fructus Mali pumilae of extract.
For achieving the above object, the technical solution used in the present invention is:
The extraction separation method of total triterpenes chemical compound in a kind of Fructus Mali pumilae, described extracting method step is as follows:
With immaturity and sophisticated Fructus Mali pumilae or apple peel, after fully cleaning up, pulverize, by liquid-solid ratio ml/g is 8-10: 1 places in the ultrasonic equipment 25～60 ℃ to extract 30-60min down and carry out ultrasonic extraction, filters then, concentrates, extraction, column chromatography is separated, and gets final product.
Above-mentioned solvent is ethanol, chloroform, ethyl acetate, butanols, acetone or water.
Extract the total triterpenes application of compound in a kind of Fructus Mali pumilae, described total triterpenes chemical compound has significant treatment and strong anti-effect to hepatocarcinoma, esophageal carcinoma, colon cancer and breast cancer cell.
Technology of the present invention is simple, and is easy and simple to handle, and raw material is easy to get, and helps the suitability for industrialized production of Fructus Mali pumilae total triterpenes chemical compound, helps the maintenance of Fructus Mali pumilae total triterpenes compound activity.The extraction separation purification is saved the energy at normal temperatures and pressures, and solvent recovery is not used and polluted.
Description of drawings
Fig. 1 is a process chart of the present invention;
The specific embodiment
With immaturity and sophisticated Fructus Mali pumilae or apple peel, after fully cleaning up, pulverize, by liquid-solid ratio ml/g is 8-10: 1 places in the ultrasonic equipment 25～60 ℃ to extract 30-60min down and carry out ultrasonic extraction, filters then, concentrates, extraction, column chromatography is separated, and gets final product.
Cleaning mainly is in order to keep extract clean hygiene free from admixture, pulverizing is convenient to extract, ultrasonic assisted extraction method is the strong cavitation effect that utilizes the ultrasound wave radiation to produce, disturbance effect, mechanical vibration, high acceleration, smash and multiple effect such as stirring, increased the frequency of molecular motion of material and the penetration power of speed and solvent, entered solvent thereby quicken target component.The using ultrasound ripple is strengthened the effective ingredient that extracts plant, is a kind of physics shattering process.Solution palpus filter and remove residue after the extraction reclaims solvent again, extracts needed object according to specification requirement, and separation and purification obtains the total triterpenes compound products then.
The present invention is a raw material with Fructus Mali pumilae and apple peel, is solvent with ethanol, chloroform, ethyl acetate, butanols, acetone or water, adopts the ultrasound wave normal pressure to extract.Extracting solution after filtration, concentrate and column chromatogram chromatography obtains the total triterpenes chemical compound.When solvent is an ethanol, raw material is a Fructus Mali pumilae when pulverizing material, is to place ultrasonic equipment at 8: 1 by liquid-solid ratio ml/g; When solvent is an ethanol, when raw material is apple peel, be to place ultrasonic equipment at 10: 1 by liquid-solid ratio ml/g.
This triterpenoid compound mainly is present among the epidermis and sarcocarp of Fructus Mali pumilae, very important to health, this compounds is playing an important role aspect prevention cancerous cell and the antioxidation, and hepatocarcinoma, esophageal carcinoma, colon cancer and breast cancer cell are had significant treatment and strong anti-effect.It not only can resist the growth of cancerous cell aspect the prevention cancerous cell, but also can reduce the quantity and the size of rat breast tumor.Aspect antioxidation, it can prevent cell and tissue because the caused infringement of free radical.It is one of most important chemical compound of minimizing and the oxidation of prevention human body cell.
China's Fructus Mali pumilae resource is very abundant, and annual production ranks first in the world, but the suitability for industrialized production of Fructus Mali pumilae triterpenoid compound still is in the starting stage.Therefore, carry out the extraction of Fructus Mali pumilae triterpenoid compound, purification, applied research, for accelerating Fructus Mali pumilae triterpenes business development paces, prolonging the Fructus Mali pumilae processing chain, increase Fructus Mali pumilae processing enterprise profit, increase the peasant economy income, reduce environmental pollution, make up harmonious civilized society, have great realistic significance and social meaning.
The present invention not only prolongs the Fructus Mali pumilae industry chain (supply chain), produces the Fructus Mali pumilae total triterpenes compound products of high added value, can also optimize the medicinal industry structure, the more important thing is the added value that promotes Fructus Mali pumilae, drives the Fructus Mali pumilae industry.
Claims (3)
1, the extraction separation method of total triterpenes chemical compound in a kind of Fructus Mali pumilae, it is characterized in that: described extraction separation step is as follows:
With immaturity and sophisticated Fructus Mali pumilae or apple peel, after fully cleaning up, pulverize, by liquid-solid ratio ml/g is 8-10: 1 places in the ultrasonic equipment 25～60 ℃ to extract 30-60min down and carry out ultrasonic extraction, filters then, concentrates, extraction, column chromatography is separated, and gets final product.
2, the extraction separation method of total triterpenes chemical compound in a kind of Fructus Mali pumilae according to claim 1, it is characterized in that: described solvent is ethanol, chloroform, ethyl acetate, butanols, acetone or water.
3, extract the total triterpenes application of compound in a kind of Fructus Mali pumilae, it is characterized in that: described total triterpenes chemical compound has significant treatment and strong anti-effect to hepatocarcinoma, esophageal carcinoma, colon cancer and breast cancer cell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2009100212589A CN101491607A (en) | 2009-02-24 | 2009-02-24 | Total triterpene compound extraction and separation method from apple and use thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
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| CNA2009100212589A CN101491607A (en) | 2009-02-24 | 2009-02-24 | Total triterpene compound extraction and separation method from apple and use thereof |
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| CN101491607A true CN101491607A (en) | 2009-07-29 |
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| CNA2009100212589A Pending CN101491607A (en) | 2009-02-24 | 2009-02-24 | Total triterpene compound extraction and separation method from apple and use thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692906A (en) * | 2009-10-16 | 2010-04-14 | 国占武 | Apple polyphenol extracting method |
| CN101810696A (en) * | 2010-04-28 | 2010-08-25 | 武汉大学 | Total triterpenoids of apples and method for preparing same |
| CN107141333A (en) * | 2017-07-10 | 2017-09-08 | 桂林融通科技有限公司 | A kind of method that ursolic acid is extracted from apple skin |
-
2009
- 2009-02-24 CN CNA2009100212589A patent/CN101491607A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101692906A (en) * | 2009-10-16 | 2010-04-14 | 国占武 | Apple polyphenol extracting method |
| CN101692906B (en) * | 2009-10-16 | 2013-11-13 | 国占武 | Apple polyphenol extracting method |
| CN101810696A (en) * | 2010-04-28 | 2010-08-25 | 武汉大学 | Total triterpenoids of apples and method for preparing same |
| CN107141333A (en) * | 2017-07-10 | 2017-09-08 | 桂林融通科技有限公司 | A kind of method that ursolic acid is extracted from apple skin |
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Open date: 20090729 |