CN101484462A - 通过定向进化产生催化优化的嵌合锌指重组酶 - Google Patents

通过定向进化产生催化优化的嵌合锌指重组酶 Download PDF

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CN101484462A
CN101484462A CNA2007800254608A CN200780025460A CN101484462A CN 101484462 A CN101484462 A CN 101484462A CN A2007800254608 A CNA2007800254608 A CN A2007800254608A CN 200780025460 A CN200780025460 A CN 200780025460A CN 101484462 A CN101484462 A CN 101484462A
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seq
recombinase
sequence
chimeric
site
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卡洛斯·F·巴巴斯三世
拉塞尔·M·高德雷
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Scripps Research Institute
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P21/00—Drugs for disorders of the muscular or neuromuscular system
    • A61P21/04—Drugs for disorders of the muscular or neuromuscular system for myasthenia gravis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P3/00—Drugs for disorders of the metabolism
    • A61P3/06—Antihyperlipidemics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P7/00—Drugs for disorders of the blood or the extracellular fluid
    • A61P7/06—Antianaemics
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1082—Preparation or screening gene libraries by chromosomal integration of polynucleotide sequences, HR-, site-specific-recombination, transposons, viral vectors
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/05—Animals comprising random inserted nucleic acids (transgenic)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/80—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor
    • C07K2319/81—Fusion polypeptide containing a DNA binding domain, e.g. Lacl or Tet-repressor containing a Zn-finger domain for DNA binding

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  • Biomedical Technology (AREA)
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  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
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  • Physical Education & Sports Medicine (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Neurology (AREA)
  • Obesity (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
CNA2007800254608A 2006-07-05 2007-07-05 通过定向进化产生催化优化的嵌合锌指重组酶 Pending CN101484462A (zh)

Applications Claiming Priority (2)

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US81890806P 2006-07-05 2006-07-05
US60/818,908 2006-07-05

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CN101484462A true CN101484462A (zh) 2009-07-15

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Country Link
US (1) US8685687B2 (enExample)
EP (1) EP2046806A4 (enExample)
JP (1) JP2009542228A (enExample)
KR (1) KR20090031938A (enExample)
CN (1) CN101484462A (enExample)
AU (1) AU2007269048A1 (enExample)
CA (1) CA2667974A1 (enExample)
WO (1) WO2008006028A2 (enExample)

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104781404A (zh) * 2012-09-04 2015-07-15 斯克利普斯研究院 具有靶向结合特异性的嵌合多肽
CN104789555A (zh) * 2015-04-29 2015-07-22 江南大学 一种基于合成单链dna文库进化基因表达调控元件的方法
CN105814573A (zh) * 2013-09-27 2016-07-27 科德克希思公司 基于结构的预测性建模
CN106232827A (zh) * 2014-02-21 2016-12-14 哈佛学院董事及会员团体 变构蛋白的从头设计
CN108138164A (zh) * 2015-08-21 2018-06-08 孟山都技术公司 基因组基因座的增强的重组
US10696964B2 (en) 2013-09-27 2020-06-30 Codexis, Inc. Automated screening of enzyme variants

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2822302A1 (en) 2006-02-13 2007-08-23 Alethia Biotherapeutics Inc. Methods of impairing osteoclast differentiation
US8168181B2 (en) 2006-02-13 2012-05-01 Alethia Biotherapeutics, Inc. Methods of impairing osteoclast differentiation using antibodies that bind siglec-15
MY160435A (en) * 2008-06-12 2017-03-15 Univ Putra Malaysia A novel antiviral peptide against avian influenza virus h9n2
WO2010042894A1 (en) * 2008-10-09 2010-04-15 The Regents Of The University Of California NOVEL NUCLEIC ACID CONSTRUCTS CONTAINING ORTHOGONAL SITE SELECTIVE RECOMBINASES (OSSRs)
GB2467167B (en) * 2009-01-26 2013-09-11 Algentech Sas Gene targeting in plants
NZ598457A (en) 2009-08-03 2014-06-27 Recombinetics Inc Methods and compositions for targeted gene modification
US20130005590A1 (en) * 2011-06-06 2013-01-03 The Regents Of The University Of California Synthetic biology tools
WO2013163628A2 (en) * 2012-04-27 2013-10-31 Duke University Genetic correction of mutated genes
US9738879B2 (en) 2012-04-27 2017-08-22 Duke University Genetic correction of mutated genes
WO2014012165A1 (en) 2012-07-19 2014-01-23 Alethia Biotherapeutics Inc. Anti-siglec-15 antibodies
US10793867B2 (en) * 2013-03-15 2020-10-06 Monsanto Technology, Llc Methods for targeted transgene-integration using custom site-specific DNA recombinases
US9828582B2 (en) 2013-03-19 2017-11-28 Duke University Compositions and methods for the induction and tuning of gene expression
JP7085716B2 (ja) 2013-06-05 2022-06-17 デューク ユニバーシティ Ｒｎａガイド遺伝子編集及び遺伝子調節
JP6303195B2 (ja) * 2013-11-20 2018-04-04 国立研究開発法人産業技術総合研究所 細菌による機能的外来タンパク質の製造方法
WO2016081924A1 (en) 2014-11-20 2016-05-26 Duke University Compositions, systems and methods for cell therapy
EP3256487A4 (en) 2015-02-09 2018-07-18 Duke University Compositions and methods for epigenome editing
JP6905755B2 (ja) 2015-08-25 2021-07-21 デューク ユニバーシティ Ｒｎａガイド型エンドヌクレアーゼを使用してゲノム工学における特異性を改善する組成物および方法
EP4089175A1 (en) 2015-10-13 2022-11-16 Duke University Genome engineering with type i crispr systems in eukaryotic cells
MX2018005377A (es) 2015-11-30 2018-11-09 Univ Duke Dianas terapeuticas para la correccion del gen de la distrofina humana por edicion genica y metodos de uso.
CN106011162A (zh) * 2016-01-27 2016-10-12 浙江大学 大肠杆菌细胞内原位进化目标蛋白质的新方法
WO2017180915A2 (en) 2016-04-13 2017-10-19 Duke University Crispr/cas9-based repressors for silencing gene targets in vivo and methods of use
EP3487523B1 (en) 2016-07-19 2023-09-06 Duke University Therapeutic applications of cpf1-based genome editing
CN111727259B (zh) 2017-12-01 2024-04-19 编码治疗公司 工程化的dna结合蛋白
CN109979591B (zh) * 2019-03-12 2021-01-01 众安信息技术服务有限公司 一种基于图神经网络分析斑块进展因子的方法及装置
CN114174520B (zh) * 2019-05-29 2025-07-08 编码治疗公司 用于选择性基因调节的组合物和方法
US11987791B2 (en) 2019-09-23 2024-05-21 Omega Therapeutics, Inc. Compositions and methods for modulating hepatocyte nuclear factor 4-alpha (HNF4α) gene expression
CN110868598B (zh) * 2019-10-17 2021-06-22 上海交通大学 基于对抗生成网络的视频内容替换方法及系统
TW202132565A (zh) * 2019-11-01 2021-09-01 美商聖加莫治療股份有限公司 Ｇｉｎ重組酶變異體
WO2025006468A2 (en) 2023-06-26 2025-01-02 University Of Hawaii Evolved integrases and methods of using the same for genome editing

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1303930A (zh) * 1999-10-27 2001-07-18 上海博容基因开发有限公司 一种新的多肽——锌指蛋白57和编码这种多肽的多核苷酸
WO2005107790A1 (en) * 2004-04-30 2005-11-17 Poetic Genetics, Llc Hybrid recombinases for genome manipulation

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB0222229D0 (en) * 2002-09-25 2002-10-30 Univ Glasgow Mutant recombinases
WO2006078431A2 (en) * 2004-12-22 2006-07-27 The University Of Iowa Research Foundation Compositions and methods related to modified retroviral vectors for restricted, site specific integration

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1303930A (zh) * 1999-10-27 2001-07-18 上海博容基因开发有限公司 一种新的多肽——锌指蛋白57和编码这种多肽的多核苷酸
WO2005107790A1 (en) * 2004-04-30 2005-11-17 Poetic Genetics, Llc Hybrid recombinases for genome manipulation

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104781404A (zh) * 2012-09-04 2015-07-15 斯克利普斯研究院 具有靶向结合特异性的嵌合多肽
CN104781404B (zh) * 2012-09-04 2018-10-26 斯克利普斯研究院 具有靶向结合特异性的嵌合多肽
CN109517068A (zh) * 2012-09-04 2019-03-26 斯克利普斯研究院 具有靶向结合特异性的嵌合多肽
US10696964B2 (en) 2013-09-27 2020-06-30 Codexis, Inc. Automated screening of enzyme variants
US11535845B2 (en) 2013-09-27 2022-12-27 Codexis, Inc. Automated screening of enzyme variants
CN105814573A (zh) * 2013-09-27 2016-07-27 科德克希思公司 基于结构的预测性建模
US11342046B2 (en) 2013-09-27 2022-05-24 Codexis, Inc. Methods and systems for engineering biomolecules
CN106232827A (zh) * 2014-02-21 2016-12-14 哈佛学院董事及会员团体 变构蛋白的从头设计
US10920217B2 (en) 2014-02-21 2021-02-16 President And Fellows Of Harvard College De novo design of allosteric proteins
CN104789555A (zh) * 2015-04-29 2015-07-22 江南大学 一种基于合成单链dna文库进化基因表达调控元件的方法
CN108138164B (zh) * 2015-08-21 2022-05-10 孟山都技术公司 基因组基因座的增强的重组
CN108138164A (zh) * 2015-08-21 2018-06-08 孟山都技术公司 基因组基因座的增强的重组
US12460217B2 (en) 2015-08-21 2025-11-04 Monsanto Technology Llc Enhanced recombination of genomic loci

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AU2007269048A1 (en) 2008-01-10
WO2008006028A2 (en) 2008-01-10
JP2009542228A (ja) 2009-12-03
KR20090031938A (ko) 2009-03-30
US8685687B2 (en) 2014-04-01
EP2046806A2 (en) 2009-04-15
EP2046806A4 (en) 2009-10-21
CA2667974A1 (en) 2008-01-10
WO2008006028A3 (en) 2008-12-24
US20100086532A1 (en) 2010-04-08

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Application publication date: 20090715