CN101473874A - Method for extracting lipid from naked oats - Google Patents
Method for extracting lipid from naked oats Download PDFInfo
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- CN101473874A CN101473874A CNA2009100664730A CN200910066473A CN101473874A CN 101473874 A CN101473874 A CN 101473874A CN A2009100664730 A CNA2009100664730 A CN A2009100664730A CN 200910066473 A CN200910066473 A CN 200910066473A CN 101473874 A CN101473874 A CN 101473874A
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Abstract
The invention provides a method for extracting grease from avena nuda. Dry avena nuda powder is put in a container; a solvent is added in the powder, wherein, the proportion between the solvent volume (ml) and the mass (g) of the avena nuda powder ranges from 5:1 to 10:1; ultrasonic extraction is carried out, wherein, the extraction temperature is 10-30 DEG C and the extraction time is 1-3h; centrifugal separation is carried out after the extraction is completed, wherein, the parameter of the centrifuge is 6000-10000r/min, the temperature of the centrifugal separation is 15-25 DEG C, and the time of the centrifugal separation is 10-60min; the solvent is eliminated by evaporation to obtain the grease extracted from the avena nuda; and the solvent is n-hexane, ethyl acetate or petroleum ether. The amount of the extracted grease is as 1.4-1.6 times great as the amount of the grease obtained by other extraction methods, the efficiency is improved, the extraction time is greatly reduced and the extraction cost is reduced; besides, the extracted avena nuda grease has excellent DPPH free radical elimination capability and the half elimination rate (IC50) of the free radical is 0.00675g/ml.
Description
Technical field
The present invention relates to from naked oats, extract the method for grease.
Background technology
Naked oats is compared with other cereal, has good nutritive value.Fatty Main Ingredients and Appearance in the naked oats is a linoleic acid.Linoleic acid is the active ingredient of blood lipid-lowering medicine.Have effect hypotensive, triglyceride reducing, can soften capillary, effects such as prevention vascular sclerosis, delaying human body caducity.So how a large amount of grease constituents that extract from naked oats have important researching value and application prospects.
The method of extracting the grease constituents from naked oats that adopts at present has apparatus,Soxhlet's extraction method (Wei Jue etc., food science and technology: 2006 (7): 204-206) and CO
2Supercritical extraction (He Wenbing etc., food industry science and technology: 2007 (4): 97-99).Naked oats and naked oats wheat bran with pulverizing are raw material, with apparatus,Soxhlet's and CO
2The supercritical extract instrument extracts oil compounds from naked oats, equipment is required height, is subjected to instrument restriction extracted amount little, and cost is also high.
Summary of the invention
In order to solve the problem that prior art exists, the invention provides the method for from naked oats, extracting grease.
The step and the condition of the method for extracting grease from naked oats provided by the invention are as follows: the naked oats powder of drying is placed container, by solvent volume ml: oat powder quality g is that the proportioning of 5:1～10:1 adds solvent, carrying out ultrasonic wave extracts, extracting temperature is 10～30 ℃, time is 1～3 hour, extract and finish the back centrifugation, centrifuge parameters is 6000～10000r/min, the centrifugation temperature is 15～25 ℃, the centrifugation time is 10～60min, the grease that extracts that obtains is removed in the solvent evaporation from naked oats; Described solvent is n-hexane, ethyl acetate or benzinum.
The present invention tests with the following oil compounds antioxidation activity method of testing that provides, and it is dry straight.
The present invention is with the following oil compounds antioxidation activity method of testing that provides: (hexichol is for bitter taste diazanyl free radical 2 for DPPH, 2-diphenyl-1-picry-hydrazylradical) free radical be a kind of stable be the proton free radical at center with nitrogen, its ethanolic solution is purple, and at the 515nm place strong absorption is arranged.When having free radical scavenger to exist, free radical scavenger provides the lone pair electrons pairing of an electronics and DPPH, and it is faded, the electronics of fading extent and its acceptance is quantitative relationship, absorbance at the 515nm place diminishes, its intensity of variation and radicals scavenging degree are linear dependence, and promptly the removing free radical ability of free radical scavenger is strong more, and absorbance is more little.Because oil compounds generally is not dissolved in the ethanol, so be difficult to directly utilize the DPPH method to record the antioxidation activity of oil compounds, the present invention adopts n-hexane: ethyl acetate: the methyl alcohol volume ratio is 1:0.5～3:1～5 mixed solvents, the grease constituents that dissolving is extracted, detect proof through DU800 type ultraviolet-visible spectrophotometer, because n-hexane, ethyl acetate and methyl alcohol itself does not remove the ability of DPPH free radical, so this solvent system does not disturb experiment, and the grease constituents that proves the oat that this invention is extracted by experiment has the ability of good removing DPPH free radical, and calculates clearance rate by formula 1;
Formula 1: clearance rate=[1-A
1/ A
0] * 100%
In the formula, A
0Be the DPPH that do not add sample absorbance, A at the 515nm place
1Be the absorbance at non-oxidizability material and the reacted 515nm of DPPH place, and calculate free radical half clearance rate.
Beneficial effect: the present invention saves solvent than conventional method, records the fat oil that is extracted and be the about 1.4-1.6 of other extracting methods doubly, has improved efficient and has significantly reduced extraction time and reduced extraction cost; And the naked oats grease that extracts has the ability of good removing DPPH free radical, free radical half clearance rate (IC
50) be 0.00675g/ml.
Description of drawings
Fig. 1 is the block diagram of the output of the grease that extracts of Different Extraction Method acquisition from naked oats.Its center pillar 1 is a traditional extraction process; Post 2 is for being the ultrasonic extracting method of solvent with ethyl acetate; Post 3 is for being the ultrasonic extracting method of solvent with the n-hexane.Method of the present invention as can be seen can increase substantially the output of the grease that extracts from naked oats.
Fig. 2 is the polymerization kinetics curves of the oil stain removing DPPH free radical that extracts that adopts mixed solvent provided by the invention and record and the negative control of solvent system from naked oats.The grease that extracts from naked oats of the present invention as can be seen has the ability of good removing DPPH free radical from contrast figure.
The specific embodiment
Embodiment 1
(1) from naked oats, extracts grease
With the naked oats powder of drying, cross 60 mesh sieves, accurately weighing 5.0134g is put to 50ml ground triangular flask, gets 3 parallel sample, adds 25ml ethyl acetate respectively, and 15 ℃ of following ultrasonic waves extract 1h.Extract is 8000r/min, centrifugal 30min 17 ℃ the time in high-speed refrigerated centrifuge, and supernatant is crossed 0.45 μ m filter membrane, merges extract in the Rotary Evaporators decompression and solvent recovery, the grease that obtains extracting from naked oats.Scales/electronic balance weighing, sample average weight are 0.1862g.
(2) sample detection
Electronic balance accurately takes by weighing the oil compounds 0.1g that extracts in the naked oats, and ((v): (v): methyl alcohol (v)=1:2:3) in the solution, gets the mixed solution of 0.1g/ml oil compounds to ethyl acetate to n-hexane to be dissolved in the 1ml mixed solvent; Electronic balance accurately takes by weighing DPPH pressed powder 2.5mg, be dissolved in the above-mentioned mixed solvent of 100ml, extracting sample solution 0.05ml and DPPH solution 1.95ml place in the 2ml cuvette, ultraviolet-visible spectrophotometer detects absorbance over time under the 515nm, records the grease that extracts from the naked oats free radical half clearance rate (IC to DPPH
50) be 0.00795g/ml.
(1) from naked oats, extracts grease
The naked oats sample of drying is processed into powder, crosses 60 mesh sieves, accurately weighing 5.0214g is put to 50ml ground triangular flask, gets 3 parallel sample, adds 50ml ethyl acetate respectively, carries out ultrasonic wave and extracts 2h for 25 ℃.Extract is 6000r/min, centrifugal 40min 20 ℃ the time in high-speed refrigerated centrifuge, and supernatant is crossed 0.45 μ m filter membrane, merges extract in the Rotary Evaporators decompression and solvent recovery, the grease that obtains extracting from naked oats.Scales/electronic balance weighing, sample average weight are 0.2278g.
(2) sample detection
Electronic balance accurately takes by weighing the oil compounds 0.1g that extracts in the naked oats, and ((v): (v): methyl alcohol (v)=1:1:3) in the solution, gets the mixed solution of 0.1g/ml oil compounds to ethyl acetate to n-hexane to be dissolved in the 1ml mixed solvent; Electronic balance accurately takes by weighing DPPH pressed powder 2.5mg, be dissolved in the above-mentioned mixed solvent of 100ml, extracting sample solution 0.05ml and DPPH solution 1.95ml place in the 2ml cuvette, ultraviolet-visible spectrophotometer detects absorbance over time under the 515nm, records the grease that extracts from the naked oats free radical half clearance rate (IC to DPPH
50) be 0.00760g/ml.
(1) from naked oats, extracts grease
The naked oats sample of drying is processed into powder, crosses 60 mesh sieves, accurately weighing 5.0179g is put to 50ml ground triangular flask, gets 3 parallel sample, adds the 25ml n-hexane respectively, and 25 ℃ of following ultrasonic waves extract 1h.Extract is 10000r/min, centrifugal 30min 17 ℃ the time in high-speed refrigerated centrifuge, and supernatant is crossed 0.45 μ m filter membrane, merges extract in the Rotary Evaporators decompression and solvent recovery, the grease that obtains extracting from naked oats.Scales/electronic balance weighing, sample average weight are 0.2588g.
(2) sample detection
Electronic balance accurately takes by weighing the oil compounds 0.1g that extracts in the naked oats, and ((v): (v): methyl alcohol (v)=1:1:3) in the solution, gets the mixed solution of 0.1g/ml oil compounds to ethyl acetate to n-hexane to be dissolved in the 1ml mixed solvent; Electronic balance accurately takes by weighing DPPH pressed powder 2.5mg, be dissolved in the above-mentioned mixed solvent of 100ml, extracting sample solution 0.05ml and DPPH solution 1.95ml place in the 2ml cuvette, ultraviolet-visible spectrophotometer detects absorbance over time under the 515nm, records the grease that extracts from the naked oats free radical half clearance rate (IC to DPPH
50) be 0.00765g/ml.
Embodiment 4
(1) from naked oats, extracts grease
The naked oats sample of drying is processed into powder, crosses 60 mesh sieves, accurately weighing 5.0258g is put to 50ml ground triangular flask, gets 3 parallel sample, adds the 40ml n-hexane respectively, and ultrasonic wave extracts 3h under 30 ℃ of low temperature.Extract is 10000r/min, centrifugal 60min 20 ℃ the time in high-speed refrigerated centrifuge, and supernatant is crossed 0.45 μ m filter membrane, merges extract in the Rotary Evaporators decompression and solvent recovery, the grease that obtains extracting from naked oats.Scales/electronic balance weighing, sample average weight are 0.2671g.
(2) sample detection
Electronic balance accurately takes by weighing the oil compounds 0.1g that extracts in the naked oats, and ((v): (v): methyl alcohol (v)=1:1:2) in the solution, gets the mixed solution of 0.1g/ml oil compounds to ethyl acetate to n-hexane to be dissolved in the 1ml mixed solvent; Electronic balance accurately takes by weighing DPPH pressed powder 2.5mg, be dissolved in the above-mentioned mixed solvent of 100ml, extracting sample solution 0.05ml and DPPH solution 1.95ml place in the 2ml cuvette, ultraviolet-visible spectrophotometer detects absorbance over time under the 515nm, records the grease that extracts from the naked oats free radical half clearance rate (IC to DPPH
50) be 0.00675g/ml.
Embodiment 5
(1) from naked oats, extracts grease
The naked oats sample of drying is processed into powder, crosses 60 mesh sieves, accurately weighing 5.0031g is put to 50ml ground triangular flask, gets 3 parallel sample, adds the 50ml benzinum respectively, and 10 ℃ of following ultrasonic waves extract 2h.Extract is 8000r/min, centrifugal 45min 20 ℃ the time in high-speed refrigerated centrifuge, and supernatant is crossed 0.45 μ m filter membrane, merges extract in the Rotary Evaporators decompression and solvent recovery, the grease that obtains extracting from naked oats.Scales/electronic balance weighing, sample average weight are 0.2398g.
(2) sample detection
Electronic balance accurately takes by weighing the oil compounds 0.1g that extracts in the naked oats, and ((v): (v): methyl alcohol (v)=1:1:2.5) in the solution, gets the mixed solution of 0.1g/ml oil compounds to ethyl acetate to n-hexane to be dissolved in the 1ml mixed solvent; Electronic balance accurately takes by weighing DPPH pressed powder 2.5mg, be dissolved in the above-mentioned mixed solvent of 100ml, extracting sample solution 0.05ml and DPPH solution 1.95ml place in the 2ml cuvette, ultraviolet-visible spectrophotometer detects absorbance over time under the 515nm, records the grease that extracts from the naked oats free radical half clearance rate (IC to DPPH
50) be 0.00710g/ml.
Embodiment 6
(1) from naked oats, extracts grease
The naked oats sample of drying is processed into powder, crosses 60 mesh sieves, accurately weighing 5.0011g is put to 50ml ground triangular flask, gets 3 parallel sample, adds the 25ml benzinum respectively, and 20 ℃ of following ultrasonic waves extract 3h.Extract is 6000r/min, centrifugal 45min 30 ℃ the time in high-speed refrigerated centrifuge, and supernatant is crossed 0.45 μ m filter membrane, merges extract in the Rotary Evaporators decompression and solvent recovery, the grease that obtains extracting from naked oats.Scales/electronic balance weighing, sample average weight are 0.1983g.
(2) sample detection
Electronic balance accurately takes by weighing the oil compounds 0.1g that extracts in the naked oats, and ((v): (v): methyl alcohol (v)=1:1:2.5) in the solution, gets the mixed solution of 0.1g/ml oil compounds to ethyl acetate to n-hexane to be dissolved in the 1ml mixed solvent; Electronic balance accurately takes by weighing DPPH pressed powder 2.5mg, be dissolved in the above-mentioned mixed solvent of 100ml, extracting sample solution 0.05ml and DPPH solution 1.95ml place in the 2ml cuvette, ultraviolet-visible spectrophotometer detects absorbance over time under the 515nm, records the grease that extracts from the naked oats free radical half clearance rate (IC to DPPH
50) be 0.00998g/ml.
Claims (1)
1, from naked oats, extracts the method for grease, it is characterized in that step and condition are as follows: the naked oats powder of drying is placed container, by solvent volume ml: oat powder quality g is that the proportioning of 5:1～10:1 adds solvent, carrying out ultrasonic wave extracts, extracting temperature is 10～30 ℃, time is 1～3 hour, extract and finish the back centrifugation, centrifuge parameters is 6000～10000r/min, the centrifugation temperature is 15～25 ℃, the centrifugation time is 10～60min, and the grease that extracts that obtains is removed in the solvent evaporation from naked oats; Described solvent is n-hexane, ethyl acetate or benzinum.
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|---|---|---|---|
| CNA2009100664730A CN101473874A (en) | 2009-01-21 | 2009-01-21 | Method for extracting lipid from naked oats |
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|---|---|---|---|
| CNA2009100664730A CN101473874A (en) | 2009-01-21 | 2009-01-21 | Method for extracting lipid from naked oats |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101846611A (en) * | 2010-06-03 | 2010-09-29 | 西北农林科技大学 | Method for determining oil content of vegetable oil material based on ultrasonic assistant extraction |
| CN101884361A (en) * | 2010-06-18 | 2010-11-17 | 武汉工业学院 | Separated oat protein preparation process |
| CN101649003B (en) * | 2009-09-09 | 2011-06-15 | 中国农业科学院农产品加工研究所 | Method for extracting beta-hyskon, starch, protein and grease from oat |
| CN102613316A (en) * | 2012-04-06 | 2012-08-01 | 无锡德冠生物科技有限公司 | Method for extracting grease from oat bran |
| CN103122273A (en) * | 2013-01-14 | 2013-05-29 | 河南科技大学 | Method for extracting oat oil through microwave pretreatment |
| CN109846795A (en) * | 2019-01-30 | 2019-06-07 | 广州诗美化妆品有限公司 | A kind of Oat Oil Extract and the preparation method and application thereof with reparation skin barrier |
-
2009
- 2009-01-21 CN CNA2009100664730A patent/CN101473874A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101649003B (en) * | 2009-09-09 | 2011-06-15 | 中国农业科学院农产品加工研究所 | Method for extracting beta-hyskon, starch, protein and grease from oat |
| CN101846611A (en) * | 2010-06-03 | 2010-09-29 | 西北农林科技大学 | Method for determining oil content of vegetable oil material based on ultrasonic assistant extraction |
| CN101884361A (en) * | 2010-06-18 | 2010-11-17 | 武汉工业学院 | Separated oat protein preparation process |
| CN102613316A (en) * | 2012-04-06 | 2012-08-01 | 无锡德冠生物科技有限公司 | Method for extracting grease from oat bran |
| CN103122273A (en) * | 2013-01-14 | 2013-05-29 | 河南科技大学 | Method for extracting oat oil through microwave pretreatment |
| CN109846795A (en) * | 2019-01-30 | 2019-06-07 | 广州诗美化妆品有限公司 | A kind of Oat Oil Extract and the preparation method and application thereof with reparation skin barrier |
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Application publication date: 20090708 |