CN101469307B - Cloning apparatus, and segmentation and acquisition mechanism thereof - Google Patents
Cloning apparatus, and segmentation and acquisition mechanism thereof Download PDFInfo
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- CN101469307B CN101469307B CN2007103054554A CN200710305455A CN101469307B CN 101469307 B CN101469307 B CN 101469307B CN 2007103054554 A CN2007103054554 A CN 2007103054554A CN 200710305455 A CN200710305455 A CN 200710305455A CN 101469307 B CN101469307 B CN 101469307B
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Abstract
The invention discloses a cloning device and a splitting and acquiring mechanism. The cloning device is used for cloning cells or tissue agglomerates, and comprises a stand, a feed mechanism and the splitting and acquiring mechanism, wherein the stand is provided with a platform and is used for laying the cells or the tissue agglomerates; the feed mechanism is arranged on the stand and can move relative to the platform; the splitting and acquiring mechanism is arranged on the feed mechanism and comprises a first cutter and a second cutter; and the second cutter is connected with the first cutter and can perform reciprocating movement between a first position and a second position along the direction of the first cutter. The feed mechanism drives the splitting and acquiring mechanism to move on the platform and align to one part of a cell or a tissue agglomerate; and after the feed mechanism drives the first cutter to cut into the part, the second cutter moves from the first position to the second position, and the part is limited between the first cutter and the second cutter.
Description
Technical field
The present invention relates to a kind of cloning apparatus, particularly a kind of cloning apparatus that utilizes gripping formula cutter cutting and choose the cell or tissue agglomerate.
Background technology
The cultivation of human embryonic stem cells is that cell mass is cultivated on the trophocyte, and when carrying out succeeding transfer culture, cell mass must cut earlier, and the part cell mass of again the desire choosing being grown is transplanted to new trophocyte and is upward continued to cultivate.Present transplanting operation major part still is dependent on manual work and is carried out, yet it is not only consuming time highly to rely on manual work, and also causes cytodifferentiation and pollution.
Known also have utilize the mechanical type instrument to select the operation of growing; Cell branch as the TaiWan, China patented claim is disclosed for No. 93141042 is grown device; Be to utilize cutting mechanism earlier cell mass to be cut apart; To transplant again in the mechanism acquisition to the accurate cell mass of having cut apart one of them, to draw this cell mass.
Above-mentioned design must again by choosing transplanting in the cell mass that has cut, can't directly be carried out the choosing of privileged site and grow earlier with the cutting of cell mass array, and above-mentioned cutting mechanism separates operation separately with transplanting mechanism, can't cut and choose cell mass simultaneously.
Summary of the invention
The present invention provides a kind of cloning apparatus, in order to select cell colonization or to organize agglomerate, comprises support, feed mechanism and cuts apart acquisition mechanism.Support has platform, in order to put the cell or tissue agglomerate.Feed mechanism is arranged on the support, and can move with respect to platform.Cut apart acquisition mechanism and be arranged on the feed mechanism, comprise first cutter and second cutter, second cutter is connected with first cutter and can between the first location and the second position, the direction towards first cutter moves back and forth.It is mobile in the platform top that acquisition mechanism is cut apart in the feed mechanism drive; Make and cut apart the part that acquisition mechanism is aimed at the cell or tissue agglomerate; And drive first cutter after this part incision; Second cutter moves to the second position by first location, and this part is limited between first cutter and second cutter, after feed mechanism drives and to cut apart acquisition mechanism and move to another desired location (be set at usually aim at another cultivate carrier); Second cutter moves to first location towards the reverse direction of first cutter by the second position; Cell mass is separated with first cutter and second cutter, and fall into another petridish, accomplish the cell or tissue agglomerate fast with the robotization mode and capture accurately fast, move and put operation.
The present invention provides a kind of acquisition mechanism of cutting apart, and in order to cut apart acquisition cell or tissue agglomerate, comprises first cutter and second cutter.First cutter comprises first blade and second blade, and first blade and second blade laterally arrange.Second cutter is connected with first cutter, and can court rotate relatively with this first blade direction and first cutter parallel with second blade.Wherein the dual-side of second cutter respectively with first blade and the second blade butt.
For letting above-mentioned and other purposes of the present invention, characteristic and the advantage can be more obviously understandable, the hereinafter spy enumerates preferred embodiment, and conjunction with figs., elaborates as follows:
Description of drawings
Fig. 1 shows the synoptic diagram of cloning apparatus of the present invention.
The synoptic diagram of acquisition mechanism is cut apart in Fig. 2 A, 2B demonstration the present invention.
Fig. 3 A show among Fig. 2 A along A-A ' tangent line sectional view.
Fig. 3 B shows the sectional view of cutting apart acquisition mechanism one variant among Fig. 2 A.
The synoptic diagram of another embodiment of acquisition mechanism is cut apart in Fig. 4 A, 4B demonstration the present invention.
Fig. 5 A show among Fig. 4 A along B-B ' tangent line sectional view.
Fig. 5 B shows the sectional view of cutting apart acquisition mechanism one variant among Fig. 4 A.
Description of reference numerals
10 cloning apparatus, 20 supports
22 feed mechanisms 23,23 ' are cut apart acquisition mechanism
The 23A first cutter 23B second cutter
30 optics camera assembly A1, first blade
The A2 second blade A3 the 3rd blade
B1 blade B1 ' blade part
B4 four blade sword B5 the 5th blade
B14, B15 blade B14 ', B15 ' blade part
The part of C cell or tissue agglomerate CP cell or tissue agglomerate
D cell or tissue agglomerate carrier P platform
Embodiment
Referring to Fig. 1, Fig. 2 A and Fig. 2 B; The present invention provides a kind of cloning apparatus 10; In order to select cell colonization or to organize agglomerate C, like human embryonic stem cells, comprise support 20, feed mechanism 22, cut apart acquisition mechanism 23 and optics camera assembly 30 in order to what cut apart acquisition cell or tissue agglomerate C.
Support 20 has transparent platform P; Carrier (petridish, the slide etc.) D of cell or tissue agglomerate C is housed in order to placement; Optics camera assembly 30 is arranged in the support 20; And being positioned at the below of platform P, the available platform P of seeing through picked-up is provided with the image of cell mass C on it, is about the 1-3 millimeter like human embryonic stem cells diameter.Feed mechanism 22 is arranged on the support 20, and can move with respect to platform P in directions X, Y direction and Z direction.Cut apart acquisition mechanism 23 and be arranged on the feed mechanism 22, when feed mechanism 22 moves with respect to platform P, drive simultaneously and cut apart acquisition mechanism 23 and move.
Behind the image of optics camera assembly 30 acquisition cell mass C, see through the computing compare of analysis of a unit, controllable feeding mechanism 22 moves, and drives and cuts apart a part of CP that acquisition mechanism 23 is aimed at cell mass C exactly, the position that desire choosing is just grown.
Cut apart acquisition mechanism 23 and comprise the first cutter 23A and the second cutter 23B (shown in Fig. 2 A, 2B).The second cutter 23B is located in the first cutter 23A; Its blade part can back and forth rotate between the first location (shown in Fig. 2 A) and the second position (shown in Fig. 2 B) towards the direction of the first cutter 23A; With the portion C P of cutting and the selected cell mass of gripping, be that the example size is about the 0.01-1 square millimeter with human embryonic stem cells.
Referring to Fig. 2 A, 2B and Fig. 3 A, the first cutter 23A comprises the first blade A1, the second blade A2 and the 3rd blade A3 simultaneously, and wherein the first blade A1 and the second blade A2 laterally arrange and be interconnected with one another through the 3rd blade A3.The second cutter 23B comprises blade B1 and blade part B1 ', both sides and the first blade A1 and the second blade A2 butt of blade part B1 ' and blade part B1 ' vertical with the B1 of blade portion, and the shape of blade part B1 ' is identical or complementary with the 3rd blade A3.
It should be noted that in present embodiment, the blade part B1 ' of the 3rd blade A3 and the second cutter 23B is linear, but is not limited thereto, shown in Fig. 3 B, the blade part B1 ' of the 3rd blade A3 and the second blade 23B can also be complementary arc or other shapes.
Consult Fig. 2 A, 2B once more; Aimed at the portion C P of cell mass C when cutting apart acquisition mechanism 23; Feed mechanism 22 moves on the Y axle, drives the first cutter 23A of cutting apart acquisition mechanism 23 and cuts this portion C P towards the direction of this cell mass C, and then the second cutter 23B rotates to the second position (shown in Fig. 2 B) by first location; Utilize the continuation of blade part B1 ' to rotate after making blade part B1 ' incision cell mass C; This portion C P of cell mass C is scooped up, this portion C P of cell mass C is separated with petridish D, and this portion C P restriction is located between the first cutter 23A and the second cutter 23B; And; Since the dual-side of the second cutter 23B respectively with the first blade A1 and the second blade A2 butt, in the second cutter 23B towards the first cutter 23A in the process of rotation, can make this portion C P of the cell mass C that desire transplants complete scooped up (shown in Fig. 2 B) by the second cutter 23B.
Referring to Fig. 4 A, 4B and 5A, 5B figure, in another embodiment of the present invention, the first cutter 23A of cutting apart acquisition mechanism 23 ' can comprise the first blade A1 and the second blade A2, and the second cutter 23B can comprise four blade sword B4 and the 5th blade B5.Four blade sword B4 and the 5th blade B5 are located in the first cutter 23A respectively; And comprise blade B14, B15 and blade part B14 ', B15 ' respectively; The blade part B14 ' of four blade sword B4 and the 5th blade B5, B15 ' are vertical with blade B14, B15 all respectively; And the dual-side of blade part B14 ', B15 ' also respectively with the first blade A1 and the second blade A2 butt, the blade part B14 ' of four blade sword B4 is identical or complementary with the shape of the blade part B15 ' of the 5th blade B5.
It should be noted; In present embodiment, blade part B14 ', the B15 ' of four blade sword B4 and the 5th blade B5 are linear, but are not limited thereto; Shown in Fig. 5 B, blade part B14 ', the B15 ' of four blade sword B4 and the 5th blade B5 can also be complementary arc or other shapes.
Consult Fig. 4 A, 4B once more; Aimed at the portion C P of cell mass C when cutting apart acquisition mechanism 23; Feed mechanism 22 moves on the Y axle, drives the first cutter 23A of cutting apart acquisition mechanism 23 and cuts this portion C P towards the direction of this cell mass C, and then four blade sword B4 and the 5th blade B5 of the second cutter 23B rotate to the second position (shown in Fig. 4 B) by first location; Utilize the continuation of blade part B14 ', B15 ' to rotate after making blade part B14 ', B15 ' incision cell mass C respectively; This portion C P of cell mass C is scooped up, this portion C P of cell mass C is separated with petridish D, and this portion C P restriction is located between the first cutter 23A and the second cutter 23B; And; Since the dual-side of four blade sword B4 and the 5th blade B5 respectively with the first blade A1 and the second blade A2 butt, in the second cutter 23B towards the first cutter 23A in the process of rotation, can make this portion C P of cell mass C of desire transplanting complete scooped up (shown in Fig. 4 B) by the second cutter 23B.
Narration cell mass C is by the action of cutting apart acquisition mechanism 23,23 ' cutting and gripping of different aspects in the foregoing description; After this portion C P of cell mass C is located between the first cutter 23A and the second cutter 23B; After feed mechanism 22 drives and cuts apart acquisition mechanism 23,23 ' and move to another desired location (be set at usually and aim at another petridish); The second cutter 23B moves to first location towards the reverse direction of the first cutter 23A by second position rotation; The portion C P of cell mass C is separated with the first cutter 23A and the second cutter 23B, and fall into another petridish.
In cloning apparatus of the present invention, the cutter of cutting apart acquisition mechanism all can adjust according to the agglomerate cut lengths, and the part of the arbitrary desire acquisition of aligning cell mass is transplanted; Get rid of other unnecessary cell masses; Can effectively utilize each part of cell, in addition, move the design of two mechanisms compared to known needs; The present invention design one can move separately cut apart acquisition mechanism; Dwindle the overall volume of cell cloning apparatus, and improve the cell mass rate travel, and can reduce the contaminated probability of cell.
Though the present invention discloses as above with preferred embodiment; Right its is not in order to limit the present invention; Those skilled in the art are not breaking away from the spirit and scope of the present invention, and when can doing a little change and retouching, so protection scope of the present invention is when looking being as the criterion that accompanying Claim defines.
Claims (25)
1. cloning apparatus in order to select cell colonization or to organize agglomerate, comprising:
Support has platform, in order to put this cell or tissue agglomerate:
Feed mechanism is arranged on this support, and can move with respect to this platform;
Cut apart acquisition mechanism, be arranged on this feed mechanism, comprising:
First cutter; And
Second cutter, being connected with this first cutter also can be towards being somebody's turn to do between the first location and the second position
The direction of first cutter moves back and forth;
Wherein this feed mechanism drives this and cuts apart acquisition mechanism and move in this platform top; Make this cut apart the part that acquisition mechanism is aimed at this cell or tissue agglomerate; And drive this first cutter after this part incision of this cell or tissue agglomerate; Second cutter moves to this second position by this first location, can make complete being scooped up by this second cutter of this part of the cell mass that desire transplants, and this part of this cell or tissue agglomerate is limited between this first cutter and this second cutter.
2. cloning apparatus as claimed in claim 1 also comprises the optics camera assembly, is arranged at this support.
3. cloning apparatus as claimed in claim 2; Wherein this this cell or tissue of optics camera assembly fechtable is organized the image of agglomerate; And see through the computing compare of analysis of a unit; To control this feed mechanism and this cutting acquisition mechanism, accurately cutting captures the part of this cell or tissue agglomerate.
4. cloning apparatus as claimed in claim 1, wherein this feed mechanism can move with respect to this platform on directions X, Y direction and Z direction, drives this and cuts apart acquisition mechanism and move with respect to this cell or tissue agglomerate.
5. cloning apparatus as claimed in claim 1; Wherein this second cutter shaft is located on this first cutter; This this cell or tissue agglomerate of first cutter incision; This second cutter rotates towards this first cutter, makes this part of this cell or tissue agglomerate opened, separate and be located in by shovel between this first cutter and this second cutter.
6. cloning apparatus as claimed in claim 5, wherein the dual-side of this second cutter respectively with this first cutter butt.
7. cloning apparatus as claimed in claim 5, wherein this first cutter comprises first blade, second blade and the 3rd blade, this first blade is parallel with this second blade and be connected with the 3rd blade respectively.
8. cloning apparatus as claimed in claim 7, wherein the dual-side of this second cutter respectively with this first blade and this second blade butt.
9. cloning apparatus as claimed in claim 7, wherein this second cutter comprises blade and blade part, and the shape complementarity of this blade part of this second cutter and the 3rd blade.
10. cloning apparatus as claimed in claim 5; Wherein this first cutter comprises first blade, second blade; And this first blade is parallel with this second blade, and this second cutter comprises four blade sword and the 5th blade, is arranged between this first blade and this second blade; And this four blade sword and the 5th blade can be located between this first blade, this second blade, this four blade sword and the 5th blade this part of this cell or tissue agglomerate towards direction rotation against each other.
11. cloning apparatus as claimed in claim 10, wherein the dual-side of this four blade sword and the 5th blade respectively with this first blade and this second blade butt.
12. cloning apparatus as claimed in claim 10, wherein this four blade sword and the 5th blade comprise blade and blade part respectively, and this blade part is vertical with this blade.
13. cloning apparatus as claimed in claim 12, the wherein shape complementarity of this blade part of this blade part of this four blade sword and the 5th blade.
14. cloning apparatus as claimed in claim 1, wherein this second cutter comprises blade and blade part, and this blade part is vertical with this blade.
15. cloning apparatus as claimed in claim 14, wherein this blade part of this second cutter and this first cutter are complementary.
16. cloning apparatus as claimed in claim 1, wherein when this second cutter when the reverse direction of this first cutter moves to this first location by this second position, this cell or tissue agglomerate and this first cutter with and/or this second cutter separate.
17. cut apart acquisition mechanism for one kind,, comprising in order to cut apart acquisition cell or tissue agglomerate:
First cutter comprises first blade and second blade, and this first blade and this second blade laterally arrange; And
Second cutter is connected with this first cutter, and can court rotate relatively with this first blade direction and this first cutter parallel with this second blade, can make complete being scooped up by this second cutter of part of the cell mass of desire transplanting;
Wherein the dual-side of this second cutter respectively with this first blade and this second blade butt.
18. the acquisition mechanism of cutting apart as claimed in claim 17, wherein this first cutter also comprises the 3rd blade, is connected with this first blade and this second blade respectively, and this second cutter is arranged between this first blade and this second blade.
19. the acquisition mechanism of cutting apart as claimed in claim 18, wherein this second cutter comprises blade and blade part, and this blade part is vertical with this blade.
20. the acquisition mechanism of cutting apart as claimed in claim 19, the wherein shape complementarity of this blade part of this second cutter and the 3rd blade.
21. the acquisition mechanism of cutting apart as claimed in claim 17, wherein and this second cutter also comprise four blade sword and the 5th blade, and the dual-side of this four blade sword and the 5th blade respectively with this first blade and this second blade butt.
22. the acquisition mechanism of cutting apart as claimed in claim 21, wherein this four blade sword and the 5th blade can rotate towards direction each other relatively.
23. the acquisition mechanism of cutting apart as claimed in claim 21, wherein this four blade sword and the 5th blade comprise blade and blade part respectively, and this blade part is vertical with this blade.
24. the acquisition mechanism of cutting apart as claimed in claim 21, the wherein shape complementarity of this blade part of this blade part of this four blade sword and the 5th blade.
25. the acquisition mechanism of cutting apart as claimed in claim 17, wherein this second cutter comprises blade and blade part, and this blade part is vertical with this blade.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007103054554A CN101469307B (en) | 2007-12-28 | 2007-12-28 | Cloning apparatus, and segmentation and acquisition mechanism thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2007103054554A CN101469307B (en) | 2007-12-28 | 2007-12-28 | Cloning apparatus, and segmentation and acquisition mechanism thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101469307A CN101469307A (en) | 2009-07-01 |
| CN101469307B true CN101469307B (en) | 2012-07-04 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2007103054554A Expired - Fee Related CN101469307B (en) | 2007-12-28 | 2007-12-28 | Cloning apparatus, and segmentation and acquisition mechanism thereof |
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| Country | Link |
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| CN (1) | CN101469307B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102206574B (en) * | 2011-04-27 | 2013-01-09 | 中国人民解放军第四军医大学 | Automatic cutting device used for primary culture of cells and lysis of tissues |
| CN107758262B (en) * | 2017-10-10 | 2024-05-24 | 重庆飞象智能装备科技有限公司 | Can realize cyclic automated production line of carrier |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1796543A (en) * | 2004-12-29 | 2006-07-05 | 财团法人工业技术研究院 | Device for micro cutting tissue of biological cells |
-
2007
- 2007-12-28 CN CN2007103054554A patent/CN101469307B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1796543A (en) * | 2004-12-29 | 2006-07-05 | 财团法人工业技术研究院 | Device for micro cutting tissue of biological cells |
Non-Patent Citations (1)
| Title |
|---|
| JP特开平5－237120A 1993.09.17 |
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| CN101469307A (en) | 2009-07-01 |
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