CN101439093A - Use of flavones substance extracted from leaves of Broussonetia papyrifera in preparing medicament for resisting fungal infection of skin - Google Patents
Use of flavones substance extracted from leaves of Broussonetia papyrifera in preparing medicament for resisting fungal infection of skin Download PDFInfo
- Publication number
- CN101439093A CN101439093A CN 200810231595 CN200810231595A CN101439093A CN 101439093 A CN101439093 A CN 101439093A CN 200810231595 CN200810231595 CN 200810231595 CN 200810231595 A CN200810231595 A CN 200810231595A CN 101439093 A CN101439093 A CN 101439093A
- Authority
- CN
- China
- Prior art keywords
- broussonetia papyrifera
- leaf
- vent
- flavonoid
- flavonoid substances
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the application of flavonoid extracted from broussonetia papyrifera leaves in preparing an anti-skin mycotic infection medicine. The flavonoid can be effectively used in preparing the anti-skin mycotic infection medicine and solves the medicine problem of anti-skin mycotic infection. The invention has the following technical proposal to solved that broussonetia papyrifera leaves are taken, crashed, whipped into coarse meal, added with ethanol, heated and refluxed so as to extract extracting solution; solvent is decompressed, concentrated, and recycled until being free of ethanol taste; the obtained concentrated solution is sequentially extracted with aether, ethyl acetate, normal butanol, and water; the position of the normal butanol is taken and the normal butanol is recycled, thus obtaining broussonetia papyrifera leave flavonoid extractum, namely, obtaining the flavonoid of anti-skin mycotic infection, the yield of which is 0.2%; the flavonoid is dissolved in a defined amount water, added with tween-80 and uniformly dissolved to obtain solution liquid which contains 2g of flavonoid in every milliliter. The method of the invention is simple; the obtained flavonoid has high purity and good stability, is effectively used in preparing anti-skin mycotic infection medicine, a greater innovation in traditional Chinese medicine and pharmacology and brings benefits to human being.
Description
One, technical field
The present invention relates to field of medicaments, a kind of especially application of from leaf of Broussonetia papyrifera (L.) L.Her.ex Vent., extracting a kind of Flavonoid substances at preparation anti-dermatophyte infection medicine.
Two, technical background
Leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. derives from the blade of Moraceae structure platymiscium Broussonetia papyrifera (Broussonetia papyrifera (L1) Vent.), and leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. has another name called: structure leaf, Broussonetia papyrifera leaf, and nature and flavor: sweet, cool, effect: clearing away heat and cooling blood, convergence dampness removing.Be generally used for epistaxis, spit blood, metrorrhagia, enteritis, dysentery, nebulousurine, eczema, successive dynasties book on Chinese herbal medicine and medication among the people show that all it has the treatment dermatosis, as tinea, pruritus due to damp pathogen, effects such as tinea skin ulcer, the Compendium of Material Medica cloud ": curing rheumatism swelling ...; the tinea skin ulcer ", cutaneous fungal infection is a kind of common disease, someone discovers, the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. ethanol extraction has the effect of anti-fungal infection, alkaloid in the prompting leaf of Broussonetia papyrifera (L.) L.Her.ex Vent., glycoside and aglycon, the material that the terpenoid isopolarity is stronger has antibacterial activity, variation and chemical sproof enhancing along with bacterial strain, the side effect of chemicals itself in addition, the application of chemicals is restricted, thereby makes numerous scholars carry out big quantity research, but do not see so far to have and from leaf of Broussonetia papyrifera (L.) L.Her.ex Vent., extract the report that Flavonoid substances is used to prepare the anti-dermatophyte infection medicine the Chinese herbal medicine antifungal.Broussonetia papyrifera distributes very extensive in China, the crude drug source abundance, can from leaf of Broussonetia papyrifera (L.) L.Her.ex Vent., extract the medicine that is used to develop the anti-dermatophyte infection that makes new advances so? if can be successful, not only can make full use of resource, reduce the misery of dermatosis patient, can also create more large economy benefit and social benefit.
Three, summary of the invention
At above-mentioned situation, the present invention's purpose just provides a kind of application of extracting a kind of Flavonoid substances at preparation anti-dermatophyte infection medicine from leaf of Broussonetia papyrifera (L.) L.Her.ex Vent., can be effective to prepare the medicine that anti-dermatophyte infects, solve the medicine problem that anti-dermatophyte infects, the technical scheme of its solution is, get leaf of Broussonetia papyrifera (L.) L.Her.ex Vent., pulverize, break into coarse powder, add mass concentration that 10-8 doubly measures leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder bulking value and be 70% ethanol, heating and refluxing extraction 2 times, each two hours, merge twice extracting solution, concentrating under reduced pressure reclaims solvent to there not being the alcohol flavor, the gained concentrated solution, use ether successively, ethyl acetate, n-butyl alcohol, n-butanol portion is got in the water extraction, reclaims n-butyl alcohol, get broussonetia papyrifera leaf flavonoids class extractum, promptly get the Flavonoid substances that anti-dermatophyte infects, yield is 0.2%, is dissolved in it in suitable quantity of water and adds tween 80 dissolving evenly, add tween 80 3g dissolving in the Flavonoid substances that every 200g leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder extracts, get lysate, make every milliliter of lysate contain the 2g Flavonoid substances, as medicinal liquid, medicinal liquid is through 100 ℃, the 45min sterilization, 4 ℃ of preservations are standby then; Said bulking value is meant that solid matter (leaf of Broussonetia papyrifera (L.) L.Her.ex Vent.) uses g, and liquid (ethanol) is used ml, and as the ethanol of 10 times of amounts of leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. bulking value, promptly the 1g leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. adds 10ml ethanol, with this roughly the same.The inventive method is easy, and gained Flavonoid substances purity is up to 95%, good stability is effective to prepare the medicine that anti-dermatophyte infects, and has opened up the frontier that leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. prepares medicine, is that one on the Chinese medicine and pharmacy innovated greatly, brings benefit to the mankind.
Four, the specific embodiment
Below in conjunction with practical situation the specific embodiment of the present invention is elaborated.
In concrete enforcement, the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder 200g after the desirable pulverizing of the present invention, the mass concentration that adds 10 times of amount leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder bulking values is 70% ethanol 2000ml, heating and refluxing extraction two hours is standby; Get mass concentration that the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder adds 8 times of amount leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder bulking values again and be 70% ethanol 1600ml, heating and refluxing extraction two hours, merge twice extracting solution, concentrating under reduced pressure reclaims solvent to not having alcohol flavor, the concentrated solution that gained extracts, use ether successively, ethyl acetate, n-butyl alcohol, the water extraction, get n-butanol portion, reclaim n-butyl alcohol, get broussonetia papyrifera leaf flavonoids class extractum, be dissolved in it in suitable quantity of water and add tween 80 3g, be dissolved to 100ml, get lysate, make lysate become every milliliter of medicinal liquid that contains the 2g Flavonoid substances, medicinal liquid is through 100 ℃, the 45min sterilization, 4 ℃ of preservations, standby.
Flavonoid substances of the present invention is effective to prepare the medicine that anti-dermatophyte infects, and is proved by test, and relevant testing data is as follows:
Through following contrast test, the result shows that the present invention is more stable to various fungus inhibitory action:
1, the making of pharmaceutical culture medium: medicinal liquid 2ml of the present invention is added 8ml sabouraud's agar (peptone 10g, 1000 milliliters in water, agar 15g, glucose 40g) mixing in, making becomes the pharmaceutical culture medium that final concentration is 400mg/ml, and the packing Boiling tube also does to wait doubly dilution, promptly first pipe to the, eight pipes are followed successively by 400mg/ml, 200mg/ml, 100mg/ml, 50mg/ml, 25mg/ml, 12.5mg/ml, 6.25mg/ml, the concentration of 3.125mg/ml.
2, the preparation of contrast medicinal liquid: get miconazole nitrate ointment (every gram contains 20 milligrams of miconazole nitrates) 10g, be dissolved in the suitable quantity of water and add several tween 80 solubilisings, be settled to 100ml at last, making miconazole nitrate concentration is 0.2g/ml.100 ℃, 45min sterilization back cryopreservation.
3, the making of control medium: miconazole nitrate culture medium concentration is for containing miconazole nitrate 20mg/ml; Other establishes the blank culture medium that does not contain any antimicrobial component.
4, for trying strain: trichophyton (Trichophyton rubrum), gram Rou Shi candidiasis (Candidiasiskrusei), phialophora compactum (Fonsecaea compactum), trichophyton gypseum (Trichophytonmentagrophytes), phialophora dermatitis (Exophiala dermatitides), sporotrichum schenckii (Sporothrix schenckii), smoking color aspergillosis (Aspergillus fumigatus), Candida albicans (Candidiasis albicans), Cryptococcus histolyticus (Cryptococcus neoformans), Oidium tropicale (Candidiasis tropicalis).
Get each culture medium test tube, be divided into Flavonoid substances group of the present invention, matched group, blank group, put into high-pressure sterilizing pot and sterilize, must be after the sterilization at 37 ℃ of following constant temperature culture 24h, determine asepsis growth, can use, inoculate corresponding strain, put in 28 ℃ of incubators and cultivate with the inclined-plane inocalation method, observe after 5 days, do not see the minimal inhibitory concentration (MIC) of the least concentration of colony growth for this position with naked eyes, by observing, the fungistatic effect of leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. different solvents extract flavone class material is as follows:
The minimum inhibitory concentration of broussonetia papyrifera leaf flavonoids extractum (mg/ml)
+++expression colony growth is vigorous; ++ the expression bacterium colony is obvious, but it is vigorous to be not so good as blank group; + expression has fragmentary or a small amount of bacterium colony to exist; Fungus is suppressed in-expression.And through test of many times (more than 20 times), and, all obtained identical or similar result to the test of different skin fungal infection, show that anti-dermatophyte of the present invention infects stable performance, reliable, has clinical meaning.
In sum, broussonetia papyrifera leaf flavonoids class extract all has inhibitory action to the sick pathomycete of various skin.By above test, flavone extract is 173.85 to the standard deviation of 10 kinds of pathomycete MIC values as calculated, not only antimicrobial spectrum is wider to show in the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. flavone compound, and it is more stable to various fungus inhibitory action, so it is comparatively desirable that it suppresses dermatosis pathomycete effect, because it derives from natural plant composition, toxic and side effects is less relatively, has the potentiality that exploitation becomes extensive pedigree antibiotic.
Claims (3)
1, a kind ofly from leaf of Broussonetia papyrifera (L.) L.Her.ex Vent., extract a kind of Flavonoid substances, it is characterized in that, said this Flavonoid substances is by leaf of Broussonetia papyrifera (L.) L.Her.ex Vent., pulverize, break into coarse powder, add mass concentration that 10-8 doubly measures leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder bulking value and be 70% ethanol, heating and refluxing extraction 2 times, each two hours, merge twice extracting solution, concentrating under reduced pressure, reclaim solvent to there not being the alcohol flavor, the gained concentrated solution is used ether successively, ethyl acetate, n-butyl alcohol, n-butanol portion is got in the water extraction, reclaim n-butyl alcohol, get broussonetia papyrifera leaf flavonoids class extractum, promptly get the Flavonoid substances that anti-dermatophyte infects, yield is 0.2%, be dissolved in it in suitable quantity of water and add tween 80 and dissolve evenly, add tween 80 3g dissolving in the Flavonoid substances that every 200g leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder extracts, get lysate, lysate contains the 2g Flavonoid substances for every milliliter.
2, according to claim 1ly from leaf of Broussonetia papyrifera (L.) L.Her.ex Vent., extract a kind of Flavonoid substances, it is characterized in that, said this Flavonoid substances is by leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder 200g, the mass concentration that adds 10 times of amount leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder bulking values is 70% ethanol 2000ml, heating and refluxing extraction two hours, standby; Get mass concentration that the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder adds 8 times of amount leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. coarse powder bulking values again and be 70% ethanol 1600ml, heating and refluxing extraction two hours, merge twice extracting solution, concentrating under reduced pressure, reclaim solvent to there not being the alcohol flavor, the concentrated solution that gained extracts, successively with ether, ethyl acetate, n-butyl alcohol, water extraction, get n-butanol portion, reclaim n-butyl alcohol, get broussonetia papyrifera leaf flavonoids class extractum, be dissolved in it in suitable quantity of water and add tween 80 3g, be dissolved to 100ml, get lysate, lysate contains the 2g Flavonoid substances for every milliliter.
3, claim 1 or 2 is describedly extracted a kind of Flavonoid substances, the application that is to prepare the anti-dermatophyte infection medicine from leaf of Broussonetia papyrifera (L.) L.Her.ex Vent..
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810231595 CN101439093A (en) | 2008-12-31 | 2008-12-31 | Use of flavones substance extracted from leaves of Broussonetia papyrifera in preparing medicament for resisting fungal infection of skin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810231595 CN101439093A (en) | 2008-12-31 | 2008-12-31 | Use of flavones substance extracted from leaves of Broussonetia papyrifera in preparing medicament for resisting fungal infection of skin |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101439093A true CN101439093A (en) | 2009-05-27 |
Family
ID=40723791
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200810231595 Pending CN101439093A (en) | 2008-12-31 | 2008-12-31 | Use of flavones substance extracted from leaves of Broussonetia papyrifera in preparing medicament for resisting fungal infection of skin |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101439093A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103037880A (en) * | 2010-07-02 | 2013-04-10 | 株式会社爱茉莉太平洋 | Composition containing paper mulberry extracts |
| CN105198945A (en) * | 2015-10-30 | 2015-12-30 | 河南中医学院 | Novel broussonetia papyrifera lactone B with antitumor effect as well as preparation method and application thereof |
| CN107307006A (en) * | 2017-07-13 | 2017-11-03 | 徐星桥 | Suppress the method for aspergillus versicolor using paper mulberry milk manufactured goods |
-
2008
- 2008-12-31 CN CN 200810231595 patent/CN101439093A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103037880A (en) * | 2010-07-02 | 2013-04-10 | 株式会社爱茉莉太平洋 | Composition containing paper mulberry extracts |
| CN105198945A (en) * | 2015-10-30 | 2015-12-30 | 河南中医学院 | Novel broussonetia papyrifera lactone B with antitumor effect as well as preparation method and application thereof |
| CN107307006A (en) * | 2017-07-13 | 2017-11-03 | 徐星桥 | Suppress the method for aspergillus versicolor using paper mulberry milk manufactured goods |
| CN107307006B (en) * | 2017-07-13 | 2020-04-10 | 徐星桥 | Method for inhibiting aspergillus versicolor by using paper mulberry milk product |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Van Wyk | A broad review of commercially important southern African medicinal plants | |
| Mamadalieva et al. | Recent advances in genus Mentha: Phytochemistry, antimicrobial effects, and food applications | |
| Touré et al. | Phytochemical screening and in vitro antifungal activities of extracts of leaves of Morinda morindoides (Morinda, Rubiaceae) | |
| CN102579525A (en) | Ilex rotunda thunb extract and preparation method and application thereof | |
| Tsouh Fokou et al. | Update on medicinal plants with potency on Mycobacterium ulcerans | |
| Muschietti et al. | In vitro antifungal assay of traditional Argentine medicinal plants | |
| CN101978987B (en) | Application of herba rabdosiae rubescentis extract to preparation of medicament for treating and resisting cerebral ischemia | |
| Biabiany et al. | Antifungal activity of 10 Guadeloupean plants | |
| Sepahvand et al. | Phytotherapy in fungi and fungal disease: a review of effective medicinal plants on important fungal strains and diseases | |
| CN101439093A (en) | Use of flavones substance extracted from leaves of Broussonetia papyrifera in preparing medicament for resisting fungal infection of skin | |
| Delices et al. | Anadenanthera colubrina (Vell) Brenan: Ethnobotanical, phytochemical, pharmacological and toxicological aspects | |
| Jeruto et al. | In vitro antifungal activity of methanolic extracts of different senna didymobotrya (fresen.) HS Irwin & barneby plant parts | |
| Mpofu et al. | Interactive efficacies of Elephantorrhiza elephantina and Pentanisia prunelloides extracts and isolated compounds against gastrointestinal bacteria | |
| CN102716188A (en) | Method for extracting extract from mangnolia officinalis fruit and antibacterial application thereof | |
| CN103816210A (en) | Pueraria lobata extract and application thereof | |
| Luka et al. | Phytochemical screening and in vitro evaluation of antibacterial activity of aqueous and ethanolic extracts of root and stem bark of Bridelia ferruginea. Benth.(Euphorbiaceae) | |
| Javaid et al. | Antifungal activity of Senna occidentalis root extract against Macrophomina phaseolina and its GC-MS analysis. | |
| CN103767980A (en) | Natural plant compound prescription with preservative effect | |
| CN102188483A (en) | Extract for treating pharyngolaryngitis and preparation method thereof | |
| CN112426446A (en) | Sophora japonica extract, preparation method of pharmaceutical composition of extract and antimalarial application of extract | |
| CN105770099A (en) | Externally applied traditional Chinese medicine for treating tinea corporis and cruris and tinea manus and pedis | |
| CN101732541B (en) | Chinese medicinal sterilizing liquor for treating skin tinea and eczema and preparation method thereof | |
| Tamilselvi et al. | Studies on estimation of berberine and antimicrobial activity of different extracts of berberis aristata DC | |
| Rao et al. | Phytochemical analysis and in vitro efficacy of two edible Cassia species on selected human pathogens | |
| Saraswathi et al. | Systemic Review on Jasminum Polyanthum: https://doi. org/10.54037/WJPS. 2022.100117 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Open date: 20090527 |