CN101978987B - Application of herba rabdosiae rubescentis extract to preparation of medicament for treating and resisting cerebral ischemia - Google Patents
Application of herba rabdosiae rubescentis extract to preparation of medicament for treating and resisting cerebral ischemia Download PDFInfo
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Abstract
The invention relates to application of herba rabdosiae rubescentis extract to preparation of a medicament for treating and resisting cerebral ischemia and can effectively solve the problems of the preparation of the medicament for resisting cerebral ischemia and the realization of the application of the herba rabdosiae rubescentis extract to preparation of the medicament for treating and resisting cerebral ischemia. The herba rabdosiae rubescentis medicinal materials are soaked in a solvent and then extracted twice, the extract is merged twice, and the mixture is concentrated and dried in a water bath to form fat soluble extract which can be effectively used for preparing the medicament for treating and resisting cerebral ischemia, wherein the solvent is one of 95 volume percent ethanol or methanol, acetone and ethyl acetate. The herba rabdosiae rubescentis fat soluble extract is prepared by the following steps of: soaking the herba rabdosiae rubescentis medicinal materials in the solvent in an amount which is ten times the weight of the herba rabdosiae rubescentis medicinal materials for 30 minutes, extracting, and recovering the extract; adding the solvent in an amount which is 8 times the weight of dregs into the dregs, extracting to obtain dreg extract, merging the extract, recovering the solvent, and concentrating and drying in the water bath. The extract can solve the problem of preparing the medicament for resisting cerebral ischemia, has a simple preparation method, is easy to produce, is innovation in the medicinal value of herba rabdosiae rubescentis and has large economic and social benefit.
Description
One, technical field
The present invention relates to field of medicaments, the application of particularly a kind of Rabdosia rubescens extract in preparation treatment anti-cerebral ischemia drugs.
Two, background technology
Rabdosia rubescens is the crack rice ground herbaceous stem part of fork Rabdosia rubescens (H ems.l) Hara of Labiatae Rabdosia plant, and Henan is the main producing region.The Rabdosia rubescens bitter in the mouth, cold nature, tool heat-clearing and toxic substances removing, the effect of anti-inflammatory analgetic and antitumor.Henan is among the people to be used to treat surplus laryngopharynx swelling and pain, the esophageal carcinoma etc. existing 50 year historically, and its water of clinical report and alcohol extract have certain curative effect to the esophageal carcinoma, carcinoma of gastric cardia, hepatocarcinoma, breast carcinoma.Pharmacological research in recent years proves that fully Rabdosia rubescens has good antiinflammatory, antibiotic, analgesic activity, can suppress first type, beta hemolytic streptococcus, staphylococcus aureus etc. effectively.
Cerebral ischemia belongs to the scope of the traditional Chinese medical science " ischemia apoplexy "; Ischemic cerebrovascular is one of principal disease that threatens human health and existence; It is common ailment of middle-aged and old; Characteristics with high incidence, high prevalence, high mortality, high disability rate, high relapse rate are a kind of diseases of present keypoint control.Angiostenosis or obturation during cerebral infarction; Cause partial ischemia of cerebral tissue or necrosis; Its pathogeny belongs to the traditional Chinese medical science " blood stasis " category; It is that the master's rule of treatment is treated primary disease that the traditional Chinese medical science is always advocated to use with " blood circulation promoting and blood stasis dispelling ", uses the cerebral ischemia of " blood circulation promoting and blood stasis dispelling " decoction for the treatment and has obtained effect preferably, has obtained sure widely.
This institute is the Henan genuine medicinal materials with Rabdosia rubescens, has another name called Rabdosia rubescens (Rabdosia rubescens (Hamst)), is Labiatae Rabdosia plant, sweet-bitter flavor, cold nature.Herb is a medical herbs commonly used among the people, has heat-clearing and toxic substances removing, anti-inflammatory analgetic, effect such as invigorate blood circulation is good for the stomach.The chemical analysis analysis confirms that Rabdosia rubescens contains from a series of materials such as monoterpene, sesquiterpene, diterpene, triterpene, volatile oil, glycoside and polysaccharide.From the extract of Rabdosia rubescens leaf, isolate five kinds of diterpene-kind compounds, promptly Rabdosia rubescens first, second, third, fourth and penta element are its main active anticancer component.Main pharmacological action is that (the Rabdosia rubescens ethanol extract has obvious antibacterial action to staphylococcus aureus and alpha streptococcus for antitumor action (cell proliferation effect, anticancer DNA, RNA and proteinic synthetic and antimutagenic effect), beta-receptor blocking effect and antibacterial and anti-inflammation functions; To colibacillary effect then very a little less than; Staphylococcus albus, group B streptococcus, bacterium flexneri are shown stronger antibacterial action, act on also stronger Bacillus typhi, dysentery bacterium, Bacillus proteus.The water extract of the bright leaf of Rabdosia rubescens has broad-spectrum antibacterial activity, and gram positive bacteria and gram negative bacteria are all had inhibitory action, will compare the height of gram negative bacteria to the antibacterial activity of gram positive bacteria; Bacillus subtilis and bacillus pyocyaneus had killing action.Secondly, the Rabdosia rubescens alcohol extract has inhibitory action to rat granuloma is swollen, and mice is had analgesic activity).
Rabdosia rubescens has certain curative effect to the esophageal carcinoma, carcinoma of gastric cardia, colon cancer, hepatocarcinoma clinically, also can use with chemotherapy drugs in combination commonly used, like PN (Bleomycin A5+nitrocaphanum), cisplatin etc., potentiation is arranged.In addition, Rabdosia rubescens is to suppurative tonsillitis, and acute and chronic pharyngolaryngitis and chronic tracheitis etc. have also favorable effects.Modern study finds that also this chemical compound or preparation have effects such as lasting and demulcent maincenter suppresses, brings high blood pressure down, two-ways regulation.In addition; Rubescensin also can reduce hepatomicrosome cytochrome P-450 content; The inducing action that suppresses sodium phenobarbital and strong carcinogen trichloro biphenyl, P-450 can activate macromolecular substances such as multiple carcinogen (like nitrosamine, flavacin and polycyclic aromatic hydrocarbon class) and nucleic acid and have an effect and cause canceration.
Rabdosia rubescens has excellent activity but hypotoxic anticancer plants as a kind of for a long time, in nearly more than 20 years, receives the concern of Chinese scholars.But, also do not find its any report to cerebral ischemia up at present.
Three, summary of the invention
To above-mentioned situation; For overcoming the defective of prior art; The present invention's purpose just provides the application of a kind of Rabdosia rubescens extract in preparation treatment anti-cerebral ischemia drugs; Can effectively solve the preparation of anti-cerebral ischemia drugs, realize the application problem of Rabdosia rubescens extract in preparation treatment anti-cerebral ischemia drugs, the technical scheme of its solution is:
Get the Rabdosia rubescens medical material, put into solvent and soak, extract twice; Merge extracted twice liquid; The water-bath concentrate drying gets liposoluble extract (or claiming Rabdosia rubescens fat-soluble extractive part), and this extract can be effective to preparation treatment anti-cerebral ischemia; Said solvent is ethanol or methanol, acetone, ethyl acetate a kind of of volumetric concentration 95%, and the method for preparing of this liposoluble extract is:
Get the Rabdosia rubescens medical material, add in the solvent of 10 times of Rabdosia rubescens medical material weight and soak 30min, extract 1h, reclaim extracting solution; Medicinal residues add the solvent of 8 times of Rabdosia rubescens medical material weight again, extract 1h, get the medicinal residues extracting solution; Merge extractive liquid,, behind the recovery solvent, the water-bath concentrate drying; Get the Rabdosia rubescens liposoluble extract, this extract can effectively solve the preparation of anti-cerebral ischemia drugs, and method for preparing is simple; Being easy to produce, is the innovation on the Rabdosia rubescens medical value, and economic and social benefit is huge.
Four, the specific embodiment
Below in conjunction with concrete condition, specific embodiments of the invention elaborates.
Choose the ground herbaceous stem part of Rabdosia rubescens, available from the Jiyuan City, Henan.Get this and get Rabdosia rubescens 20 grams, add the ethanol of 200 grams 95%, soak 30min, extract 1h; Cross and filter extracting solution, medicinal residues add 95% ethanol of 160 grams again, extract 1h, cross and filter the medicinal residues extracting solution; Merge twice extracting solution, behind the recovery ethanol, water-bath concentrates; Drying, Rabdosia rubescens extract, Rabdosia rubescens extract extracts as stated above through many batches of multiple different amounts repeatedly and has all obtained identical or akin result; This method is reliable and stable, and its Rabdosia rubescens extract is effective to prepare the medicine of treating anti-cerebral ischemia, and through many batches of zooperies of repeated multiple times; Proved that fully the Rabdosia rubescens liposoluble extract has treating cerebral ischemia, realized the application of Rabdosia rubescens extract in preparation treatment anti-cerebral ischemia drugs, relevant animal data is following:
1. medicine and reagent
Rabdosia rubescens extract reagent, Rabdosia rubescens are the crack rice ground herbaceous stem part of fork Rabdosia rubescens (Hems.l) Hara of Labiatae Rabdosia plant, available from the Jiyuan City; Get the Rabdosia rubescens medical material, add the ethanol of 10 times of amounts 95%, soak 30min, extract 1h, released liquor; Medicinal residues add the ethanol of 8 times of amounts 95% again, extract 1h; Merge extractive liquid,, water-bath concentrate drying behind the recovery ethanol, dry thing adds water and processes Rabdosia rubescens extract reagent;
Dexamethasone sodium phosphate injection is produced, lot number 0907183; Jiangsu Lianshui Pharmaceutical Co., Ltd. produces; YANGXUE QINGNAO KELI is produced lot number Z10960082 by Tianjin Tasly Pharmaceutical Co., Ltd; The NOS testing cassete, Nanjing is built up bio-engineering research and is provided, lot number 20100626; The NO testing cassete, Nanjing is built up bio-engineering research and is provided, lot number 20100626; The ATP detection kit, building up bio-engineering research by Nanjing provides, lot number 20100114,20100613; The Coomassie brilliant blue testing cassete, Nanjing is built up bio-engineering research and is provided, lot number 20100626; Nimodipine tablet, universal love Pharmaceutical Co in Xi'an provides, lot number 090117; HUATUO ZAIZAO WAN, Qixing Pharmaceutical Co., Ltd., Guangzhou provides, lot number 7438.
1.2 laboratory animal
White mice, cleaning level, the Kunming kind is provided by Hebei province's Experimental Animal Center, 18～22g, male and female half and half, the quality certification numbers 912102; Animal quality certification numbering: 28～32g, male, the quality certification numbers 1006019.
1.3 experimental apparatus
Automatic blood flow graph (LBY-N6B & LBY-N6K), Pulisheng Instruments Co., Ltd., Beijing produces; The UV-2000 ultraviolet-uisible spectrophotometer, UNICO(Shanghai) Instruments Co., Ltd. produces; The TGL-16G desk centrifuge, Anting Scientific Instrument Factory, Shanghai produces; Electronic analytical balance, Ao Haosi (Shanghai) company produces; DZKW-4 type electronic thermostatic water-bath, the bright forever Medical Instruments in Beijing factory produces; DY89-1 type electric driven glass refiner, the new sesame bio tech ltd in Ningbo is produced; Adjustable pipette, Lei Bo Analytical Instrument Co., Ltd in Shanghai produces.
2. experimental technique and result
2.1 influence to mice normal pressure anoxia enduring
Get 50 of Kunming mouses, male and female half and half, body weight 19～22g; Be divided into 5 groups at random, 10 every group, irritate the Rabdosia rubescens ethanol extract suspension (0.2g/ml that obeys large, medium and small dosage respectively; 0.1g/ml, 0.05g/ml; 0.2ml/10g), (0.1g/ml is 0.2ml/10g) and with the normal saline (0.2ml/10g) of volume for the YANGXUE QINGNAO KELI suspension.Administration every day 1 time, successive administration 5 days.After the last administration 1 hour, mice is placed the 150mL wide mouthed bottle of demarcating through capacity, the built-in 10g sodica calx of bottle is in order to absorbing carbon dioxide; Bottle cap is coated with rapidly with the vaseline sealing, makes it air tight, immediately timing; With the respiratory arrest is index, the record time-to-live of mice in airtight port grinding bottle.The result sees table 1.
Table 1 Rabdosia rubescens is to the influence
of mice normal pressure anoxia enduring
* represent with model group than P＜0.05, * * represent with model group than (P＜0.01)
Can find out that from last table with blank group ratio, but the equal significance of large, medium and small dosage Rabdosia rubescens extract group and YANGXUE QINGNAO KELI group improves mice normal pressure hypoxia-bearing capability (P＜0.01), act as excellent with big or middle dosage Rabdosia rubescens extract group.
2.2 influence to the mouse brain ischemia-reperfusion injury model
Get 60 of Kunming mouses, male and female half and half, body weight 19～22g is divided into 6 groups at random, 10 every group; Wherein make the cerebral ischemia re-pouring model for 5 groups, other has 1 group to be the blank group.5 groups of Rabdosia rubescens ethanol extract suspension (0.2g/ml that irritate the large, medium and small dosage of clothes respectively of modeling type; 0.1g/ml, 0.05g/ml, 0.2ml/10g), YANGXUE QINGNAO KELI suspension (0.1g/ml; 0.2ml/10g) and with the normal saline (0.2ml/10g) of volume; The blank group is given the normal saline (0.2ml/10g) with volume, administration every day 1 time, successive administration 5 days.In last administration 1h (water 12h is can't help in fasting), 10% chloral hydrate intraperitoneal injection of anesthesia mice after mice is weighed faces upward and is fixed on the little rat holder; The cervical region sterilization, median incision, passivity is separated; Expose bilateral common carotid arteries, recover to sew up behind the perfusion behind the blocking-up bilateral common carotid arteries blood flow 30min, after each mice recovers to pour into 24h again; Take off cervical vertebra and put to death, get cerebral tissue rapidly and weigh, add 9 times of abundant homogenate of normal saline; High speed low temperature centrifugal machine is centrifugal, gets supernatant (processing sample); Say requirement mensuration cerebral tissue protein content and ATP enzyme activity according to test kit, concrete assay method is referring to attaching 1, attaching 2, and the result sees table 2.
Attaches 1 Coomassie brilliant blue protein determination Operations Sequence Chart
Mixing left standstill 10 minutes, in the 595nm place, and the 1cm optical path, the distilled water zeroing is surveyed and is respectively managed OD value protein content (g/L)=(measuring pipe OD value-blank pipe OD value) * standard pipe concentration ÷ (standard pipe OD value-blank pipe OD value)
Attaches atpase assay Operations Sequence Chart in 2 brain homogenates
(annotate: each test solution is that test kit has)
Mixing, centrifugal 10 minutes of 4000rpm gets supernatant 200 μ l and decides phosphorus
Decide phosphorus
Mixing, 37 ℃ of water-baths 30 minutes are cooled to room temperature, at 660nm, 1cm optical path distilled water zeroing colorimetric
Annotate: the A pipe is control tube; The B pipe is Na
+K
+-ATP enzyme pipe; The C pipe is Mg
++-ATP enzyme pipe; The D pipe is Ca
++-ATP enzyme pipe; Diluted sample multiple (2.5) * 6 ÷ sample protein content (mgprot/ml) in ATP vigor in the tissue (μ molPi/mgprot/hour)=(measuring pipe OD value one control tube OD value) ÷ standard pipe OD value * standard pipe concentration (0.5 μ mol/ml) * reaction system
Table 2 Rabdosia rubescens is to the influence
of mouse brain ischemia model brain homogenate ATP enzyme activity
* represent with model group than (P＜0.01)
Can find out from last table, with blank group ratio, model group Na
+-K
+-ATP, Mg
++-ATP, Ca
++-ATP and Ca
++-Mg
++-ATP vigor significantly reduces (P＜0.01), and the success of modeling type is described.With the model group ratio, but the equal significance of large, medium and small dosage Rabdosia rubescens extract group and YANGXUE QINGNAO KELI group improves mouse brain ischemia model brain homogenate Na
+-K
+ATP, Mg
++-ATP, Ca
++-ATP and Ca
++-Mg
++-ATP vigor (P＜0.01).Act as excellent with big or middle dosage Rabdosia rubescens extract group.
2.3 the mice blood stasis is merged the influence of cerebral ischemia re-pouring model
Get 216 of Kunming mouses, male, body weight 29～32g is divided into 9 groups at random, 24 every group; Wherein 1 group is that blank group, 1 group are that blood stasis model sham operated rats, 1 group are not hemopoietic stasis of blood model surgery group, and the 6 groups of whole hemopoietic stasis of blood of mice models add the operation hemopoietic stasis of blood and merge cerebral ischemic model in addition.The hemopoietic stasis of blood merges 6 groups of cerebral ischemic models; Irritate the Rabdosia rubescens ethanol extract suspension (0.2g/ml of the large, medium and small dosage of clothes respectively; 0.1g/ml, 0.05g/ml, 0.2ml/10g), the HUATUO ZAIZAO WAN suspension (0.1g/ml, 0.2ml/10g), nimodipine suspension (0.02g/ml; 0.2ml/10g) reaching normal saline (0.2ml/10g) with volume, blank group, blood stasis model sham operated rats and not hemopoietic stasis of blood model surgery group are all irritated the normal saline (0.2ml/10g) with volume.Administration every day 1 time, successive administration 16 days; Administration in the 1st day simultaneously, 7 groups of equal back leg intramuscular injection of dexamethasone 0.8mg/kg every day (2 groups of injection equal-volume normal saline of not hemopoietic stasis of blood model) of hemopoietic stasis of blood model, 15d continuously.1h after the 16d administration (water 12h is can't help in fasting), each organizes mice through 10% chloral hydrate 0.033ml/10g intraperitoneal anesthesia, lies on the back and is fixed on the operating-table; The throat median incision, passivity is separated bilateral common carotid arteries, and blank group and blood stasis model sham operated rats are done sham-operation; All the other each all close bilateral common carotid arteries 10min with arteriole folder folder, recover perfusion 10min, reuse arteriole folder folder closes bilateral common carotid arteries 10min; Recover blood supply, sew up mice.Each is organized mice 24h eye socket after operation and gets blood survey hemorheology, and the result sees table 3; Put to death mice then, get cerebral tissue rapidly and weigh, add the abundant homogenate of normal saline, high speed low temperature centrifugal machine is centrifugal, gets supernatant, says according to test kit and surveys the ATP enzyme activity, surveys NO and NOS level.Concrete assay method is referring to attaching 3, attaching 4, and the result sees table 4, table 5.
WBV operating procedure: at first adjust the instrument level.Turn on the power switch subsequently, get into preheat mode, temperature value reaches 37 when spending on the screen to be shown, the beginning application of sample.Get the 1ml whole blood with sample injector, the liquid bath inwall is close in the sample injector outlet is slowly injected downwards, in order to avoid sneak into the aeration measurement result.Click the whole blood test menu, make an experiment, detect finish after, write down that whole blood is high, normal, basic to be cut.Click clear button, after the cleaning, get into next sample test.
Attaches NO mensuration sequence list in 3 brain homogenates
(annotate: each reagent is that test kit has)
NO content in the tissue (μ mol/gprot)=(measuring the pipe absorbance-blank pipe of mensuration absorbance) * standard substance concentration ÷ (the blank pipe of standard pipe absorbance-standard absorbance) ÷ protein content (mgprot/ml)
Attaches NOS mensuration sequence list in 4 brain homogenates
(annotate: each reagent, test solution are that test kit has)
TNOS vigor (U/ml)=(TNOS measures the blank pipe of pipe-TNOS) ÷ colour generation thing nanomole extinction coefficient * (reactant liquor cumulative volume ÷ gets liquid measure) ÷ (colorimetric optical path * response time) ÷ sample protein content (mggrot/L)
INOS vigor (U/ml)=(iNOS measures the blank pipe of pipe-iNOS) ÷ colour generation thing nanomole extinction coefficient * reactant liquor cumulative volume ÷ gets liquid measure * [1 ÷ (colorimetric optical path * response time)] ÷ sample protein content (mggrot/L)
Table 3 Rabdosia rubescens extract merges the influence (
mPS) of cerebral ischemic model whole blood viscosity to the mice blood stasis
▲ ▲Expression and blank group be than P＜0.01, * * represent with model group than P＜0.0l
Can find out from last table; With blank group ratio; Not hemopoietic stasis of blood surgical groups, blood stasis not surgical groups and blood stasis add that the surgical groups blood flow is high, medium and low cuts equal significance and raise (P＜0.01), and it is the most serious to add surgical groups with blood stasis, and it is maximum that death condition also adds surgical groups with blood stasis; Explain and make the brain ischemia-reperfusion injury model and hemopoietic stasis of blood model all can make the mouse blood viscosity significantly increase, both act on stackable.Add the surgical groups ratio with blood stasis; Each administration group dead mouse situation all reduces; Large, medium and small dosage Rabdosia rubescens extract group, HUATUO ZAIZAO WAN group and nimodipine group all can make blood stasis add the high, medium and low index of cutting of hemorheology that operation raises due to the modeling significantly to reduce (P＜0.01), act as excellent with heavy dose of Rabdosia rubescens extract group.
Table 5 Rabdosia rubescens extract merges the influence
of cerebral ischemia re-pouring model brain homogenate NO and NOS level to the mice blood stasis
▲ ▲Expression and blank group be than P＜0.01, * * represent with model group than P＜0.01
Can find out from last table; With blank group ratio; Not hemopoietic stasis of blood surgical groups, blood stasis not surgical groups and blood stasis add the equal significance of surgical groups brain homogenate NO, INOS and TNOS level and raise (P＜0.01), and it is the most serious to add surgical groups with blood stasis, and it is maximum that death condition also adds surgical groups with blood stasis; Explain and make the brain ischemia-reperfusion injury model and hemopoietic stasis of blood model all can make mouse brain homogenate NO, INOS and TNOS level significantly increase, both act on stackable.Add the surgical groups ratio with blood stasis; Each administration group dead mouse situation all reduces; Large, medium and small dosage Rabdosia rubescens extract group, HUATUO ZAIZAO WAN group and nimodipine group all can make blood stasis add that rising brain homogenate NO, INOS and TNOS level significantly reduce (P＜0.01) due to the operation modeling, act as excellent with big or middle dosage Rabdosia rubescens extract group.
Table 4 Rabdosia rubescens extract merges the influence
of cerebral ischemic model brain homogenate ATP enzyme activity to the mice blood stasis
▲ ▲Expression and blank group be than P＜0.01, * * represent with model group than P＜0.01
Can find out that from last table with blank group ratio, not hemopoietic stasis of blood surgical groups, blood stasis not surgical groups and blood stasis add surgical groups blood flow brain homogenate Ca
++-ATP enzyme, Mg
++-ATP enzyme and Ca
++-Mg
++The equal significance of-ATP vigor reduces (P＜0.01); It is the most serious to add surgical groups with blood stasis; It is maximum that death condition also adds surgical groups with blood stasis, explains to make the brain ischemia-reperfusion injury model and hemopoietic stasis of blood model all can make the mouse blood viscosity significantly increase, and both act on stackable.Add the surgical groups ratio with blood stasis, each administration group dead mouse situation all reduces, and large, medium and small dosage Rabdosia rubescens extract group, HUATUO ZAIZAO WAN group and nimodipine group all can make blood stasis add the brain homogenate Ca that operation reduces due to the modeling
++-ATP enzyme, Mg
++-ATP enzyme and Ca
++-Mg
++-ATP vigor significantly raises (P＜0.01), act as excellent with heavy dose of Rabdosia rubescens extract group.
3, brief summary
According to above-mentioned method for preparing and experimental technique; The fat melting property extract that Rabdosia rubescens is made with immersions such as acetone, methanol, ethyl acetate respectively; All obtained identical or akin effect (concrete experimental technique is because of identical with the method for preparing and the experimental technique of ethanol extraction, so no longer repeat).
In a word; Liposoluble extract repeated multiple times experiment through the ethanol of Rabdosia rubescens, acetone, methanol, ethyl acetate; But fully proved Rabdosia rubescens extract significant prolongation mice normal pressure hypoxia-bearing capability; Significantly improve the energy metabolism of mice ischemia-reperfusion injury model brain homogenate, significantly reduce the mice blood stasis and merge the cerebral ischemic model hemorheology and high, medium and lowly cut level, significantly reduce brain homogenate NO, INOS and TNOS level, significantly raise brain homogenate Ca++-ATP enzyme, Mg++-ATP enzyme and Ca++-Mg++-ATP vigor.Explain that Rabdosia rubescens extract has good anti-cerebral ischemia and cerebral ischemia reperfusion injury effect.
Claims (1)
1. the application of Rabdosia rubescens extract in preparation treatment anti-cerebral ischemia drugs, said extract is that Rabdosia rubescens is by 95% alcoholic acid fat melting property extractum.
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| CN103142690A (en) * | 2011-12-07 | 2013-06-12 | 彭莉 | Rabdosia rubescens stem aqueous extract and its preparation method, finger-prints and use as antithrombotic agent |
| CN103142688A (en) * | 2011-12-07 | 2013-06-12 | 彭莉 | Rabdosia rubescens stem aqueous extract and its preparation method, finger-prints and use as anti-inflammatory agent |
| CN104127485A (en) * | 2014-07-14 | 2014-11-05 | 河南中医学院 | Application of Rabdosia rubescens extract in preparation of cerebral ischemia tolerance improving medicines |
| CN104083436A (en) * | 2014-07-24 | 2014-10-08 | 河南中医学院 | Application of rabdosia rubescens extract in preparation of hypoglycemic agent |
| CN112704676B (en) * | 2021-02-10 | 2022-05-31 | 南京医科大学 | Application of oridonin in preparation of medicine for preventing and/or treating cerebral arterial thrombosis |
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