CN101434644A - Novel method for efficiently extracting mycoprotein by microfiltration membrane - Google Patents

Abstract

The invention discloses a new method for efficiently extracting mycoprotein on the basis of a micro-filtration membrane method; the key point of the method lies in that fermentation liquid of aminophenol is firstly filtered by a filter to remove long fiber and large grains, then dialyzed cyclically by a ceramic micro-filtration membrane so as to filter out active ingredients fully, and then concentrated, atomized and dried to obtain high-quality protein powder. The method has the advantages that the extraction ratio of the protein is more than 98 percent; the adoption of the micro-filtration membrane for filtration has high automation degree and small labor intensity and greatly decreases the employee numbers and reduces labor cost. The method requires no chemical regents, improves the product quality, reduces production cost, and solves the problem the technical problem that the existing technology has the defects of high energy consumption and low protein extraction ratio, thus having a wide application range in the aminophenol industry for the mycoprotein extraction.

Description

A kind of novel method based on micro-filtration embrane method high efficiency extraction tropina

Technical field the invention belongs to the amino acid production technical field, relates to amino acid and tropina novel method in a kind of high efficiency separation fermented liquid, is specifically related to a kind of novel method based on micro-filtration embrane method high efficiency extraction tropina.

Contain impurity such as tropina and other jellies in the background technology amino acid zymotic fluid, the existence of these impurity influences amino acid whose quality and extraction yield, has also increased certain difficulty for the production of later process.Remove these impurity by pre-treatment both at home and abroad before amino acid extracts, main method is high speed centrifugation, thermal precipitation and flocculation sediment.But there is the technical problem that the high speed centrifugation energy consumption is big, the thermal precipitation effect is relatively poor, the flocculation sediment protein extracting ratio is low in prior art.

At present, domestic common employing flocculation sediment extracts tropina in the fermented liquid, and this method is to have added flocculation agent and flocculating aids in fermented liquid, treats with sheet frame it to be filtered behind the albumen precipitation, though this technology can reach less energy-consumption and effect is better than thermal precipitation technology, there are the following problems:

1. protein extracting ratio is not high.The amino acid yield is not high;

2. use Plate Filtration, labour intensity is big;

3. the post personnel are many, increase cost of labor;

4. in fermented liquid, add flocculation agent and flocculating aids, increased production cost and quality product virtually.

Membrane separation technique is as a kind of novel separation, concentrate, purification techniques, become in recent years and solved the contemporary energy, the important new and high technology of resource and environmental pollution, at present, membrane technique is in sea water desaltination, pure water preparation, liquid liquid separates, concentrate, purify, the trade effluent aspect has obtained significant progress, because membrane process is specially adapted to the processing of heat-sensitive substance, therefore, in food-processing, fields such as medicine and fine chemistry industry have unique adaptability, along with membrane technique is in recent years at home and abroad constantly promoted widely, and the film cost is applied to food also in continuous decline with membrane separation and purification technology, to produce remarkable economic efficiency in the leaching process in medicine downstream.Using membrane technique can not only improve the quality of products, and can also reduce the discharging of waste water, and the protection environment is realized automatization control and cleaner production, reduces production costs, and can also improve workman's Working environment, increases work efficiency.

Summary of the invention the objective of the invention is to have the big and low technical problem of protein extracting ratio of energy consumption in order to solve prior art, provides a kind of novel method based on micro-filtration embrane method high efficiency extraction tropina, to overcome the deficiencies in the prior art.

For achieving the above object, the present invention is based on the novel method of micro-filtration embrane method high efficiency extraction tropina, is that a kind of employing microfiltration membrane is carried out filtration sterilization to fermented liquid, with amino acid and the method for producing the high-quality tropina after tropina separates;

Its main points are to carry out according to following processing step:

1) through the aperture strainer removal macrofiber and the macrobead of 1-4mm with amino acid zymotic fluid earlier;

2), the filtrate with step 1) is the ceramic microfiltration membrane of 10-200nm by the aperture again, temperature 40-90 ℃, advance film pressure 3-8bar, go out film pressure 0-5bar, membrane flux 70-180L/m ².h dialysis circulates under the condition;

3), begin to add the water dialysis when volume multiple reaches 5-12 times, fully leach effective constituent when dialyzate concentrates, amount of water be the concentrated solution volume 1-6 doubly;

4), when elutriant stops when leaching requirement equates filtering with adding, the film system is carried out emptying, clear liquid is mainly effective constituent amino acid, concentrated solution is tropina;

5), concentrated solution carried out spraying drying produce the quality protein powder, clear liquid enters down the road abstraction process.

The invention has the beneficial effects as follows that protein extracting ratio reaches more than 98%.Use micro-filtrate membrane filtration, the level of automation height, labour intensity is little, reduces the post personnel greatly, reduces cost of labor.Need not to add any chemical reagent, improved quality product, reduced production cost.

Amino acid fermentation is a high pollution industry, and its high pollution row shows the pollution aspect of sewage discharge pollution, air.The present invention has solved that protein extracting ratio in the present separating thallus albumen technology is low, labour intensity big, the technical problem of poor product quality, but adopt high efficiency separation fox extracting thallus protein and amino acid after the technology of the present invention, non-wastewater discharge, alleviate environmental protection pressure, had remarkable social benefit and environmental benefit; The tropina of handling the back generation simultaneously is the quality protein powder owing to do not add any chemical substance after the drying, high-valued obvious, has improved economic benefit.

The present invention has following advantage:

1, adopt micro-filtration to remove thalline, the tropina rate of recovery is higher, can realize that 98% reclaims, it is high by about 20% to extract albumen than air supporting method in the traditional technology, need not add flocculating aids or flocculation agent, has improved the quality of tropina by product in the time of compliance with environmental protection requirements, increased the value of by product, it is reported that high-quality tropina price is 2550 yuan/ton, high-valued obvious;

2, save cost, supporting cost is low, need not sheet frame, device such as aseptic wind, and the level of automation height reduces cost, and is easy to operate, and labour intensity is little, saves human cost;

3, work simplification can improve the amino acid yield, and the film device floor space is little, can realize serialization production, and the efficient of production significantly improves, and has improved operating environment, and operation and maintenance are simple.

The flocculation agent that needs in the traditional technology to add is 3 ‰ sodium polyacrylate, presses 7% of material liquid volume and adds, and the market price of sodium polyacrylate is about 7000 yuan/ton, and adopting new technology does not need flocculation agent,

Therefore 1 ton of L-glutamic acid of every production is cost-saved: 10 * 7% * 3 ‰ * 7000=14.7 unit;

Abundant Feng Jituan produces 300000 tons in L-glutamic acid per year, adopt novel process of the present invention after, annual cost-saved: 14.7 * 300,000=4,410,000 yuan;

Need artificial 12 people the Plate Filtration operation every day in the traditional technology, and in the novel process of the present invention every day need artificial 3 people, according to 60 yuan of artificial every days, be 300 days meters 1 year working days, then can save every year manually: (12-3) * ten thousand yuan of 60 * 300=16.2; With abundant Feng Jituan is example, produces 300000 tons in L-glutamic acid per year, adopt novel process of the present invention after, annual cost-saved 457.2 ten thousand yuan.

In a word, but adopt high efficiency separation fox extracting thallus protein and amino acid after this technology, non-wastewater discharge, alleviate environmental protection pressure, reach closed cycle, have remarkable social benefit and environmental benefit, handle the tropina of back generation simultaneously owing to do not add any chemical substance, be the quality protein powder after the drying, high-valued obvious.And add chemical floc in the traditional technology, therefore the tropina that causes extracting can not be used as feedstuff protein, can only be used as fertilizer and sell (550 yuan/ton of market prices), therefore, but adopt production high-quality tropina (2550 yuan/ton of market prices) after the novel process of the present invention, the tropina rate of recovery is higher, can realize that 98% reclaims, it is high by about 20% to extract albumen than air supporting method in the traditional technology, with abundant Feng Jituan is example, produces 300000 tons in L-glutamic acid per year, adopt novel process of the present invention after, bring 2478.6 ten thousand yuan of direct economic benefits, and annual cost-saved 457.2 ten thousand yuan.

Therefore, the present invention has remarkable economic efficiency, social benefit and environmental benefit.

Description of drawings Fig. 1 is the process flow diagram of fox extracting thallus protein among the present invention;

Fig. 2 is a membrane flux change curve in time.

Embodiment the present invention is the technology of tropina in a kind of high efficiency extraction amino acid zymotic fluid, carries out according to following step:

1, fermented liquid 1350L enters the cassette strainer that the aperture is 2mm;

2, enter the micro-filtration ceramic membrane of aperture 50nm again,, advance film pressure 3.5bar, go out film pressure 2bar, average film flux 167L/m 55 ℃ of temperature ².h dialysis circulates under the condition;

3, reach 11.25 times when cycles of concentration, promptly during concentrated solution 120L, begin to add the water dialysis, fully leach effective constituent, amount of water is 2.5 times of concentrated solution volume, i.e. 300L;

4, when elutriant equates with amount of water, stop to filter, the film system is carried out emptying, clear liquid main component Xie Ansuan, concentrated solution is tropina;

5, the concentrated solution tropina enters the spray-drier drying and produces the quality protein powder, and clear liquid enters down the road abstraction process.

By Fig. 3 membrane flux in time change curve as can be seen, membrane flux is basicly stable at 160L/m ².h about, average flux 167L/m ².h.

Effect contrasted before and after table 1 pair micro-filtration filtered.

As can be seen from Table 1, after the 1350L fermented liquid enters the micro-filtration ceramic membrane filter, obtain dialyzate 1530L (comprising the 300L elutriant) and concentrated solution (tropina) 120L, 11.25 times of cycles of concentration, amino acid yield 98.3%, thallus removing rate 98.2%.

Effect comparison table before and after table 1 micro-filtration filters

Remarks:

1, bacteria containing amount measuring method: fermented liquid, filter back clear liquid, the elutriant 50ml that takes a sample respectively puts into whizzer with 6000rpm centrifugation 10min, incline and supernatant liquor, stay throw out, use the raw spirit washed twice again, centrifugation, inclining supernatant liquor, stays throw out, and drying, constant weight, weighing draw wet thallus content.

2, thallus removing rate method of calculation: thalline content * 100% in thallus removing rate=(in the clear liquid of thalline content in the fermented liquid-filter back in thalline content-elutriant thalline content)/fermented liquid

3, yield method of calculation: yield=(filter back clear liquid quality * filter back clear liquid acid content+elutriant quality * elutriant acid content)/(fermented liquid quality * fermented liquid acid content) * 100%

4, cycles of concentration method of calculation: cycles of concentration=filter front volume/filter back volume * 100%

Claims (1)

1, a kind of novel method based on micro-filtration embrane method high efficiency extraction tropina is characterized in that carrying out according to following processing step:

1) through the aperture strainer removal macrofiber and the macrobead of 1-4mm with amino acid zymotic fluid earlier;

2), the filtrate with step 1) is the ceramic microfiltration membrane of 10-200nm by the aperture again, temperature 40-90 ℃, advance film pressure 3-8bar, go out film pressure 0-5bar, membrane flux 70-180L/m ².h dialysis circulates under the condition;

3), begin to add the water dialysis when volume multiple reaches 5-12 times, fully leach effective constituent when dialyzate concentrates, amount of water be the concentrated solution volume 1-6 doubly;

4), when elutriant stops when leaching requirement equates filtering with adding, the film system is carried out emptying, clear liquid is mainly effective constituent amino acid, concentrated solution is tropina;

5), concentrated solution carried out spraying drying produce the quality protein powder, clear liquid enters down the road abstraction process.

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