CN101411897A - Method for preparing chitin-containing combined artificial skin in adhesive bandage - Google Patents
Method for preparing chitin-containing combined artificial skin in adhesive bandage Download PDFInfo
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- CN101411897A CN101411897A CNA2008102286493A CN200810228649A CN101411897A CN 101411897 A CN101411897 A CN 101411897A CN A2008102286493 A CNA2008102286493 A CN A2008102286493A CN 200810228649 A CN200810228649 A CN 200810228649A CN 101411897 A CN101411897 A CN 101411897A
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Abstract
The invention relates to a preparation method for chitin combined artificial skin of band-aid. A groove of a packaged substrate is provided with an inunction tube containing liquid artificial skin and the band-aid with an adsorption layer and a film layer. The liquid artificial skin in the inunction tube is prepared by the following steps: step one, preparing solution I by chitosan, acetic acid and distilled water; and soaking collagen in the distilled water, and preparing solution II after heating and dissolving the collagen; and step two, adding chitosan, boric acid and alcohol into the solution II to prepare solution III. The adsorption layer is prepared by the following steps: step one, same as the step one in the preparation of the liquid artificial skin; and step two, adding chitin sulfuric acid ester, glycerol and cuttlebone powder, stirring the mixture evenly, storing and grinding sediment, stirring the sediment in a stirrer, pouring the sediment into a mould after the sediment becomes thin foam, freezing, drying and cutting the sediment into required size. The film layer is prepared by the following steps: step one, same as the step one in the preparation of the liquid artificial skin; and step two, adding chitosan, boric acid and glycerol to prepare solution, after filtering the solution, pouring the sediment on a flat plate, drying the sediment under dustless condition, forming film, and cutting the film into required size.
Description
Technical field
The preparation method of the chitin combination artificial skin that the present invention can paste for a kind of wound.
Background technology
Skin is the vitals of human body, and skin covers whole body, and it makes interior various tissues of body and organ avoid the invasion and attack of physical property, mechanicalness, chemical and pathogenic microorganism.Skin has the barrier action of two aspects: prevent on the one hand moisture in the body, the losing of electrolyte and other materials; Stop the intrusion of extraneous harmful substance on the other hand.Keeping playing important defencive function on human internal environment stable on physiology, skin also participates in the metabolic process of human body simultaneously.
Yet, when people are engaged in vairious activities, inevitably occur damaging, injury such as wound, scald.
The medical research personnel have done number of research projects to artificial skin, it as application number 200480043831.1 patent, it be a kind of be that primary raw material is made artificial skin with collagen, and application number is a kind of artificial skin bacterial fibers wet film preparation method of 200610026708.x, it is the artificial skin made from the cellulose of the deposits yields of supporting one's family, above-mentioned two made artificial skins of application, aspects such as the affinity of its biocompatibility, skin, flexibility, antibiotic, anti-inflammatory analgetic are relatively poor.Application number is 02128433.4 a kind of artificial skin and manufacture method, be that non-woven viscose fabric is united the outer artificial skin that polyethylene glue is made that is coated with chitin fiber water thorn, mechanical strength is bigger, and certain moisture is arranged, but it pastes skin, detumescence property and relatively poor to the aspects such as seal of wound.Application number is 02100072.7, with chitin or derivant is a kind of artificial skin of substrate rack, at rack both sides inoculation epidermal growth factor and fibroblast growth factor, it is a kind of active artificial skin, suit hospital through for a long time, uses that cultivate one's ability of somatomedin under the aseptic condition, suitable to severe skin damage patient; And for go out, tourism etc. is unexpected of minor cut or wound, present adhesive bandage of the artificial skin that can paste immediately, to the temporary wrapping of wound, and long-time wrapping is unfavorable to wound healing.
In sum, need a kind of wounds after, can clear up wound at the scene, carry out the dissipation processing, can paste immediately, can promote the artificial skin of skin regeneration, accelerating wound healing.
Summary of the invention
The objective of the invention is to, solve above-mentioned existing existing problem, provide a kind of and can paste immediately, can promote the preparation method of the chitin combination artificial skin that a kind of wound of skin regeneration, accelerating wound healing can be pasted.
The present invention is achieved in that substrate and protecting film form packing, and the groove of substrate is placed and embrocated pipe, hinders and can paste; The medical cotton ball of putting in embrocating pipe contains the liquid artificial skin, embrocates the tube end sealing, and outer wall is provided with broken line; Wound can be pasted the middle part that is included in base cloth layer and be posted the artificial skin composite bed, and both sides are glue-line.Its particular point is, 1, said liquid artificial skin adopts following preparation method, and (1), chitosan (85% deacetylation) 3-5g, acetic acid 3-5ml, distilled water 400-500ml is dissolved as chitosan solution I, and is standby; (2), get collagen 3-5g and be dipped in the 100ml distilled water swelling 4 hours, join mix homogeneously among the chitosan solution I after being heated to 60 ℃ of dissolvings then, obtain mixed solution I I; (3), get oligochitosan 0.1-1g, boric acid 5g, ethanol (75%) 300-400ml again, residue is distilled water, join and be mixed with the 1000ml solution III among the mixed solution I I, (4), solution III is filtered removal impurity, insoluble matter, promptly become the liquid artificial skin, be filled to then and embrocate in the pipe, sterilize, seal.
2, the artificial skin composite bed during above-mentioned said wound can be pasted comprises that adsorption layer, book rete are composited; Its adsorption layer adopts following preparation method, (1), chitosan (85% deacetylation) .18-20g, and acetic acid 8-10ml, distilled water 700-800ml is dissolved as chitosan solution I, and is standby; (2), collagen 18-20g was dipped in the 150ml distilled water swelling 4 hours, was heated to 60 ℃ then, joined mix homogeneously among the chitosan solution I after the dissolving, obtained mixed solution I I; (3), get chitin sulfuric ester 3-5g, glycerol 8-10ml again, Endoconcha Sepiae powder 1-3g joins among the mixed solution I I and stirs, static then depositing 8-10 hours, stir putting into solution again after the precipitum taking-up grinding, till no precipitum, obtain solution III; (4), then observe the solution III uniformity coefficient, solution is provoked, be and put into blender behind the even thread shape and stir with Glass rod, 2000 rev/mins of its mixing speeds, be poured in the mould after being the Aphron shape, lyophilization under-40 ℃ of conditions is taken out and is cut into required size.
Its book rete adopts following preparation method, (1), chitosan (85% deacetylation) 18-20g, and acetic acid 8-10ml, distilled water 700-800ml is dissolved as chitosan solution I, and is standby; (2), collagen 18-20g was dipped in the 150ml distilled water swelling 4 hours, was heated to 60 ℃ then, joined mix homogeneously among the chitosan solution I after the dissolving, obtained mixed solution I I; (3), again get oligochitosan 0.1-1g, boric acid 8-10g, glycerol 8-10ml, remain to distilled water joins among the mixed solution I I and stir, be mixed with the 1000ml solution III; (4), solution III filter is removed impurity, insoluble matter, be poured on flat board (glass plate or steel plate) or the casting machine, dry under dustless condition, dry back skinning cuts into required size then.
Adsorption layer, book rete encapsulate after sterilizing.
Advantage of the present invention:
1, the effect of liquid artificial skin is, (1), cleaning wound also carry out dissipation to be handled, and when sialorrhea is paved with uneven wound, has moistening book film artificial skin layer, reaches the effect that seals whole wound; (2), acetic acid,diluted solution dissolving chitosan plays the effect of dissipation and secular artificial skin, wherein the oligochitosan of Jia Ruing more has lethality to antibacterial, and the boric acid, the ethanol that add have and sterilizes and anti-corrosion function.(2) the liquid artificial skin is encapsulated in and embrocates when use at the scene in the pipe, opens promptly and uses.
2, the adsorption layer effect is, (1), be the effusive serum albumin of absorption wound that the foam adsorption layer of main material can be more with the chitosan; (2), in chitosan, add the chitin sulfuric ester, also form the blood microcirculation passage anticoagulant; (3) add collagen, glycerol, Endoconcha Sepiae powder, for skin provides the regeneration nutrient source; (4), make up skin regeneration between adsorption layer and wound and the book rete serum and nutrient source are arranged, aseptic self skin regeneration culture environment.
3, the effect of book rete is, (1), make the liquid moistening seal whole wound; (2) make up the skin regeneration environment and between the adsorption layer, promote skin regeneration; (3), after wound attaches, tighten skin and suppress wound swelling, (4), externally play epidermis effect, good permeability; (5), in the wound healing phase, the skin that can suppress to regenerate scabs, and reduces cicatrix.
Description of drawings
Fig. 1 is a sectional structure sketch map of the present invention;
Fig. 2 is a vertical view;
The specific embodiment
Describe in detail below in conjunction with accompanying drawing: as shown in the figure, between substrate 1 and protecting film 2, the groove of substrate is placed and is embrocated pipe 3, hinders and can paste 4, can place the sterilization towel at groove 6 places in addition; Medical cotton ball 5 contains the liquid artificial skin in embrocating pipe 3, embrocates pipe 3 end seals; Embrocate pipe 3 outer walls and be provided with broken line; Wound can be pasted 4 middle parts that are included in base cloth layer and be posted the artificial skin composite bed, and both sides are glue-line.
1, said liquid artificial skin adopts following preparation method, (1), chitosan (85% deacetylation) 3-5g, and acetic acid 3-5ml, distilled water 400-500ml is dissolved as chitosan solution I, and is standby; (2), collagen 3-5g was dipped in the 100ml distilled water swelling 4 hours, joined mix homogeneously among the chitosan solution I after being heated to 60 ℃ of dissolvings then, obtained mixed solution I I; (3), get oligochitosan 0.1-1g, boric acid 5g, ethanol (75%) 300-400ml again, for corruption granulation promoting wounds such as decubital ulcers preferably, add four yellow ethanol 100ml, residue is distilled water, join and be mixed with the 1000ml solution III among the mixed solution I I, (4), solution III filter is removed impurity, insoluble matter promptly become the liquid artificial skin, be filled to then and embrocate in the pipe, sterilize, seal.
Said four yellow ethanol are with Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae, Radix Et Rhizoma Rhei, each 3-5g of Sanguis Draxonis, immerse in the 150ml ethanol (75%), take after 7-8 days.
2, the artificial skin composite bed during above-mentioned said wound can be pasted comprises that adsorption layer, book rete are composited; Its adsorption layer adopts following preparation method, (1), chitosan (85% deacetylation) 18-20g, and acetic acid 8-10ml, distilled water 700-800ml is dissolved as chitosan solution I, and is standby; (2), collagen 18-20g was dipped in the 150ml distilled water swelling 4 hours, was heated to 60 ℃ then, joined mix homogeneously among the chitosan solution I after the dissolving, obtained mixed solution I I; (3), get chitin sulfuric ester 3-5g, glycerol 8-10ml again, Endoconcha Sepiae powder 1-3g joins among the mixed solution I I and stirs, static then depositing 8-10 hour, stir putting into solution again after the precipitum taking-up grinding, till no precipitum, obtain solution III; (4), then observe the solution III uniformity coefficient, solution is provoked, be and put into blender behind the even thread shape and stir with Glass rod, 2000 rev/mins of its mixing speeds, be poured in the mould after being the Aphron shape, lyophilization under-40 ℃ of conditions is taken out and is cut into required size.
Its book rete adopts following preparation method, (1), chitosan (85% deacetylation) 18-20g, and acetic acid 8-10ml, distilled water 700-800ml is dissolved as chitosan solution I, and is standby; (2), collagen 18-20g was dipped in the 150ml distilled water swelling 4 hours, was heated to 60 ℃ then, joined mix homogeneously among the chitosan solution I after the dissolving, obtained mixed solution I I; (3), again get oligochitosan 0.1-1g, boric acid 8-10g, glycerol 8-10ml,, add traditional Chinese medicine liquid 100ml, remain to distilled water joins and stir among the mixed solution I I, be mixed with the 1000ml solution III for corruption such as decubital ulcer preferably granulation promoting wound; (4), solution III filter is removed impurity, insoluble matter, be poured on flat board (glass plate or steel plate) or the casting machine, dry under dustless condition, dry back skinning cuts into required size then.
Above-mentioned (3) step, said traditional Chinese medicine liquid was with Olibanum, each 8-10g of Myrrha, put into 300ml water, boiled 30-60 minute, used chitosan acetic acid (0.5%) solution 5-10ml clarification then; The clarification medicinal liquid is a traditional Chinese medicine liquid.
During use, open protecting film, take out and to embrocate pipe, fracture embrocating pipe 3 outer wall broken line places, promptly available liquid artificial skin is handled wound, after handling well, pastes and hinders and can paste.
Claims (3)
1, the preparation method of a kind of wound chitin combination artificial skin that can paste comprises that substrate and protecting film form packing, and the groove of substrate is placed and embrocated pipe, wound can be pasted; The liquid artificial skin is arranged in embrocating pipe, embrocate the tube end sealing, wound can be pasted the middle part that is included in base cloth layer and be posted the artificial skin composite bed, and both sides are glue-line; It is characterized in that (1), said liquid artificial skin adopt following preparation method, a, chitosan (85% deacetylation) 3-5g, acetic acid 3-5ml, distilled water 400-500ml is dissolved as chitosan solution I, and is standby; B, get collagen 3-5g and be dipped in the 100ml distilled water swelling 4 hours, join mix homogeneously among the chitosan solution I after being heated to 60 ℃ of dissolvings then, obtain mixed solution I I; C, get oligochitosan 0.1-1g, boric acid 5g, ethanol (75%) 300-400ml again, remain and be distilled water, join and be mixed with the 1000ml solution III among the mixed solution I I; D, solution III filter is removed impurity, insoluble matter, promptly become the liquid artificial skin, be filled to then and embrocate in the pipe, sterilize, seal;
(2), the artificial skin composite bed during above-mentioned said wound can be pasted comprises that adsorption layer, book rete are composited; Its adsorption layer adopts following preparation method, a, chitosan (85% deacetylation) 18-20g, and acetic acid 8-10ml, distilled water 700-800ml is dissolved as chitosan solution I, and is standby; B, collagen 18-20g were dipped in the 150ml distilled water swelling 4 hours, were heated to 60 ℃ then, joined mix homogeneously among the chitosan solution I after the dissolving, obtained mixed solution I I; C, get chitin sulfuric ester 3-5g, glycerol 8-10ml again, Endoconcha Sepiae powder 1-3g joins among the mixed solution I I and stirs, static then depositing 8-10 hour, stir putting into solution again after the precipitum taking-up grinding, till no precipitum, obtain solution III; D, observe the solution III uniformity coefficient then, solution is provoked, be and put into blender behind the even thread shape and stir with Glass rod, 2000 rev/mins of its mixing speeds, be poured in the mould after being the Aphron shape, lyophilization under-40 ℃ of conditions is taken out and is cut into required size;
Its book rete adopts following preparation method, a, chitosan (85% deacetylation) 18-20g, and acetic acid 8-10ml, distilled water 700-800ml is dissolved as chitosan solution I, and is standby; B, collagen 18-20g were dipped in the 150ml distilled water swelling 4 hours, were heated to 60 ℃ then, joined mix homogeneously among the chitosan solution I after the dissolving, obtained mixed solution I I; C, get oligochitosan 0.1-1g, boric acid 8-10g, glycerol 8-10ml again, remain to distilled water joins among the mixed solution I I and stir, be mixed with the 1000ml solution III; D, solution III filter is removed impurity, insoluble matter, be poured on flat board (glass plate or steel plate) or the casting machine, dry under dustless condition, dry back skinning cuts into required size then.
2, the chitin that can paste according to the said a kind of wound of claim 1 makes up the preparation method of artificial skin, it is characterized in that, adds four yellow ethanol 100ml at the c of liquid artificial skin preparation method in the step, is mixed with 1000ml solution; Said four yellow ethanol are with Rhizoma Coptidis, Cortex Phellodendri, Radix Scutellariae, Radix Et Rhizoma Rhei, each 3-5g of Sanguis Draxonis, immerse in the 150ml ethanol (75%), take after 7-8 days.
3, the chitin that can paste according to the said a kind of wound of claim 1 makes up the preparation method of artificial skin, it is characterized in that, adds traditional Chinese medicine liquid 100ml at the c of book rete preparation method in the step, remains to be distilled water, is mixed with 1000ml solution; Said traditional Chinese medicine liquid is with Olibanum, each 8-10g of Myrrha, puts into 300ml water, boils 30-60 minute, uses chitosan acetic acid (0.5%) solution 5-10ml clarification then, and the clarification medicinal liquid is a traditional Chinese medicine liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008102286493A CN101411897A (en) | 2008-11-06 | 2008-11-06 | Method for preparing chitin-containing combined artificial skin in adhesive bandage |
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| Application Number | Priority Date | Filing Date | Title |
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| CNA2008102286493A CN101411897A (en) | 2008-11-06 | 2008-11-06 | Method for preparing chitin-containing combined artificial skin in adhesive bandage |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791425A (en) * | 2010-03-30 | 2010-08-04 | 赵雪林 | Antibacterial heal-promoting gel material used for preparing medical wound dressing and preparation method thereof |
| CN102476477A (en) * | 2011-06-29 | 2012-05-30 | 深圳光启高等理工研究院 | Preparation method of metamaterial medium substrate |
-
2008
- 2008-11-06 CN CNA2008102286493A patent/CN101411897A/en active Pending
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101791425A (en) * | 2010-03-30 | 2010-08-04 | 赵雪林 | Antibacterial heal-promoting gel material used for preparing medical wound dressing and preparation method thereof |
| CN101791425B (en) * | 2010-03-30 | 2013-04-10 | 赵雪林 | Antibacterial heal-promoting gel material used for preparing medical wound dressing and preparation method thereof |
| CN102476477A (en) * | 2011-06-29 | 2012-05-30 | 深圳光启高等理工研究院 | Preparation method of metamaterial medium substrate |
| CN102476477B (en) * | 2011-06-29 | 2013-07-03 | 深圳光启高等理工研究院 | Preparation method of metamaterial medium substrate |
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