CN101390562A - Southernwood dregs biology protein and preparation method thereof - Google Patents
Southernwood dregs biology protein and preparation method thereof Download PDFInfo
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- CN101390562A CN101390562A CNA2007100927120A CN200710092712A CN101390562A CN 101390562 A CN101390562 A CN 101390562A CN A2007100927120 A CNA2007100927120 A CN A2007100927120A CN 200710092712 A CN200710092712 A CN 200710092712A CN 101390562 A CN101390562 A CN 101390562A
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Abstract
The invention relates to an artemisia residue biological protein and a preparation method; the main components of the artemisia residue biological protein comprise bacterial protein, enzymes, vitamins and amino acids; the preparation method for the artemisia residue biological protein has the following steps: based on weight percentage, 70-80% of artemisia residue, 10-20% of rice bran and 5-10% of nitrogen source are mixed and made into the fermentation medium; the initial moisture content of the fermentation medium is adjusted to 50-60%; the complex microbial agent at least comprising candida and trichoderma viride is inoculated in the fermentation medium which is treated through high-pressure sterilization and activation and cooled; the inoculation amount accounts for 8-9% of the fermentation medium by weight percentage; the inoculated compound is fermented for 36 hours at a temperature of 29 DEG C and at the pH value of 6.5-7.5, so as to obtain the artemisia residue biological protein; the invention adopts the cheap artemisia residue as the main material to prepare protein feed through multi-strain mixing solid state fermentation to improve the utilization rate of the artemisia residue with simple process.
Description
Technical field
The present invention relates to a kind of bioprotein and preparation method thereof, relate in particular to a kind of southernwood dregs biology protein and preparation method thereof.
Background technology
Qinghaosu is as the main component of preparation anti-malaria medicaments, and since eighties of last century was found, sweet wormwood was also more and more big in the cultivated area in the whole nation at present, and the plantation exploitation of sweet wormwood has become an industry.The sweet wormwood slag is that qinghaosu extracts the accessory substance that the back produces, qinghaosu has produced a large amount of sweet wormwood slags at the wilderness demand of preparation anti-malaria medicaments, shows according to the result of related detection analysis: contain compositions such as thick protein (about 21%), crude fibre (about 11%) and amino acid in the sweet wormwood slag.At present, the processing method of sweet wormwood slag mostly is direct burning or directly is used as the feed livestock and poultry of feeding, and directly burning causes certain pollution to environment; With the sweet wormwood slag as feed directly feed livestock and poultry also exist protein content not high, absorb problem such as bad, palatability difference, utilization rate is not high.Along with the fast development of China's animal husbandry, the raw material sources of feed begin to go short of and shortage, and especially the deficiency of protein feed has become global problem.The protein feeds that China adopts mainly is grouts and fish meal, cost an arm and a leg, and China is the country of fish meal resource shortage, and all will rely on a large amount of imports every year.So the exploitation of protein feeds has very high economic worth and practical value.Utilize the sweet wormwood slag that is rich in nutritional labelings such as protein to become problem anxious to be solved on the market better.
Summary of the invention
In view of this, the object of the present invention is to provide a kind of southernwood dregs biology protein.For realizing purpose of the present invention, southernwood dregs biology protein of the present invention mainly is made up of crude protein, crude fibre, biology enzyme, vitamin, amino acid, by weight percentage, wherein the content of crude protein greater than 50%, coarse-fibred content is less than 8%.
Another object of the present invention is to provide the method for the above-mentioned southernwood dregs biology protein of preparation, may further comprise the steps:
A. prepare fermentation medium
By weight percentage with the sweet wormwood slag: 70～80%, rice bran: 10～20%, inorganic salts: make fermentation medium after 5%～10% mixing, and the initial water content of culture medium is adjusted to 50-60% by weight percentage, it is stand-by that the autoclaving activation processing is cooled off the back more again;
B. inoculation mixes
To contain the inoculation of Candida, Trichoderma viride composite bacteria agent capable at least and be mixed in the fermentation medium, by weight percentage, the inoculum concentration of compound bacteria is the 8-9% of fermentation medium
C. fermentation
With above-mentioned inoculated mixture be in temperature that 29 ℃, Ph value are 6.5～7.5, water supply in media content is the condition bottom fermentation of 48-52% 36 hours by weight percentage, can obtain southernwood dregs biology protein;
D. dry, quality inspection
The resulting southernwood dregs biology protein in back that will ferment carries out drying to be handled, and can be used as biological protein feedstuff after the quality inspection.
Further, the nitrogenous source among the described step a is selected from a kind of in ammonium sulfate, urea, the ammoniacal liquor;
Further, the composite bacteria agent capable among the described step b also comprises bacillus, aspergillus;
Further, inoculated mixture is 29 ℃ in temperature among the described step c, and water supply in media content is 50% condition bottom fermentation 36 hours by weight percentage.
The invention has the beneficial effects as follows, adopting cheap sweet wormwood slag is primary raw material, prepare protein feeds by many bacterial classifications mixed solid fermentation, technology is simple, can effectively reduce the materials such as cellulose, hemicellulose and ANFs in the sweet wormwood slag, make it to be converted into beneficiating ingredients such as corresponding mycoprotein, crude fat, amino acid, vitamin, to substitute the part grain feed, to reduce aquaculture cost significant.
Other advantage of the present invention, target and feature will be set forth to a certain extent in the following description, and to a certain extent, based on being conspicuous to those skilled in the art, perhaps can obtain instruction from the practice of the present invention to investigating hereinafter.
Description of drawings
The present invention is described in further detail below in conjunction with accompanying drawing.
Accompanying drawing is the process chart of the inventive method
The specific embodiment
Below in conjunction with accompanying drawing, the preferred embodiments of the present invention are described in detail, rather than restriction the present invention.
Embodiment 1
Get 80 kilograms of sweet wormwood slags, 15 kilograms in rice bran, after 5 kilograms of mixing of ammonium sulfate are mixed thoroughly, make fermentation medium, the fermentation medium initial water content is adjusted to 50% by weight percentage, carry out the autoclaving activation processing again and cool off stand-by, to contain Candida at least, Trichoderma viride, bacillus, the composite bacteria agent capable of aspergillus is inoculated in the fermentation medium, by weight percentage, the inoculum concentration of compound bacteria is 8 kilograms, temperature is controlled at 29 ℃ then, the Ph value of fermentation medium is 6.5～7.5, water supply in media content is that 48% condition bottom fermentation promptly obtains required southernwood dregs biology protein product after 36 hours by weight percentage, and the resulting southernwood dregs biology protein in back that will ferment carries out drying processing, can be used as biological protein feedstuff after the quality inspection.
Embodiment 2
Get 75 kilograms of sweet wormwood slags, 15 kilograms in rice bran, after 5 kilograms of mixing of ammonium sulfate are mixed thoroughly, make fermentation medium, the fermentation medium initial water content is adjusted to 55% by weight percentage, to contain Candida at least, the composite bacteria agent capable of Trichoderma viride is inoculated in the cooled again fermentation medium of autoclaving activation processing, by weight percentage, the inoculum concentration of compound bacteria is 8.5 kilograms, temperature is controlled at 29 ℃ then, the Ph value of fermentation medium is 6.5～7.5, water supply in media content is that 50% condition bottom fermentation promptly obtains required southernwood dregs biology protein product after 36 hours by weight percentage, the resulting southernwood dregs biology protein in back that will ferment carries out drying to be handled, and can be used as biological protein feedstuff after the quality inspection.
Embodiment 3
Get 80 kilograms of sweet wormwood slags, 10 kilograms in rice bran, after 10 kilograms of mixing of ammonium sulfate are mixed thoroughly, make fermentation medium, the fermentation medium initial water content is adjusted to 60% by weight percentage, to contain Candida, Trichoderma viride, bacillus, the composite bacteria agent capable of aspergillus is inoculated in to plant after the autoclaving activation processing is cooled off again and is mixed in the fermentation medium, by weight percentage, the inoculum concentration of compound bacteria is 9 kilograms, temperature is controlled at 29 ℃ then, the Ph value of fermentation medium is 6.5～7.5, water supply in media content is that 60% condition bottom fermentation promptly obtains required southernwood dregs biology protein product after 36 hours by weight percentage, the resulting southernwood dregs biology protein in back that will ferment carries out drying to be handled, and can be used as biological protein feedstuff after the quality inspection.
In culture medium, add a certain amount of rice bran, can increase gap between matrix, be beneficial to and ventilate and oxygen supply.Water supply in media content should be controlled at fermented bacterium and can grow and be lower than the needed moisture of bacterial growth during the fermentation.In this sweat, moisture should carry out moisture and replenish owing to factors such as evaporation, bacterial metabolism activity and ventilation reduces, and adopts logical humid air or carries out adding for twice mode such as water and solve when turning over song.Because the variations in temperature of solid state fermentation is subjected to directly influencing of system radiating effect, can be by the temperature of cooling and the constant temperature recirculating cooling water system control solid state fermentation that ventilates.When temperature is higher than when temperature required, can increase ventilation speed or ventilation frequency, cool off by the pump recirculated cooling water simultaneously.When temperature is lower than when temperature required, can reduce ventilation speed or ventilation frequency, circulation constant temperature water supply system heats up.
Using method: be the protein feeds finished product after the product drying quality inspection packing after the fermentation, can be used as the protein feeds of various livestock and poultry, be used to dispose grain-saving type, low-cost animal and fowl fodder.
Toxicity test
Choosing body weight is 20 of 18～22 mouse that restrain, first fasting 16 hours, not water restriction, the southernwood dregs biology protein that allows animal free choice feeding the inventive method prepare then, after 30 days, from hematology and the blood biochemical index of being tried the abdominal aorta blood sampling chemical examination of mouse, check to be tried mouse.The hematological examination project has: hemoglobin (HGB), blood platelet (PLT), red blood cell count(RBC) (RBC), reticulocyte count, white blood cell count(WBC) (WBC), leukocyte differential count (DC), clotting time.Blood biochemical is learned inspection item to be had: asparatate aminotransferase (AST), ALT (ALT), alkaline phosphatase (ALP), urea nitrogen (BUN), total protein (TP), albumin (ALB), blood sugar (GLU), total bilirubin (T-BIL), creatinine (Grea), T-CHOL (T-CHO).Hematology and blood biochemical are learned the index no abnormality seen, to be tried mouse and be put to death the back dissection, check main organs lesion tissue situation, histoorgans such as the heart, liver,kidney,spleen, lung, adrenal gland, thyroid gland, thymus gland, testis, epididymis, ovary, uterus, brain there is no unusually.
Above-mentioned experimental result shows, this product is to livestock and poultry nonhazardous effect, uses this product livestock and poultry of feeding, and its meat does not contain harmful material.
Although by reference some preferred embodiment of the present invention, invention has been described, but those of ordinary skill in the art is to be understood that, can make various changes to it in the form and details, and the spirit and scope of the present invention that do not depart from appended claims and limited.
Claims (5)
1. southernwood dregs biology protein is characterized in that: mainly form by crude protein, crude fibre, biology enzyme, vitamin, amino acid, by weight percentage, wherein the content of crude protein greater than 50%, coarse-fibred content is less than 8%.
2. the preparation method who prepares southernwood dregs biology protein as claimed in claim 1 is characterized in that may further comprise the steps:
A. prepare fermentation medium
By weight percentage with the sweet wormwood slag: 70～80%, rice bran: 10～20%, nitrogenous source: 5%～10% makes fermentation medium after mixing, and the initial water content of culture medium is adjusted to 50%-60% by weight percentage, again autoclaving activation processing and cool off stand-by;
B. inoculation mixes
At least the composite bacteria agent capable that contains Candida, Trichoderma viride is inoculated in the above-mentioned fermentation medium, and by weight percentage, the inoculum concentration of compound bacteria is the 8-9% of fermentation medium;
C. fermentation
With above-mentioned inoculated mixture be in temperature that 29 ℃, Ph value are 6.5～7.5, water supply in media content is the condition bottom fermentation of 48-52% 36 hours by weight percentage, can obtain southernwood dregs biology protein;
D. dry, quality inspection
The resulting southernwood dregs biology protein in back that will ferment carries out drying to be handled, and can be used as biological protein feedstuff after the quality inspection.
3. the preparation method of southernwood dregs biology protein as claimed in claim 2 is characterized in that: the nitrogenous source among the described step a is selected from a kind of in ammonium sulfate, urea, the ammoniacal liquor.
4. the preparation method of southernwood dregs biology protein as claimed in claim 2, it is characterized in that: the composite bacteria agent capable among the described step b also comprises: bacillus, aspergillus.
5, the preparation method of southernwood dregs biology protein as claimed in claim 2 is characterized in that: inoculated mixture is that 29 ℃ of water supply in media content are 50% condition bottom fermentation 36 hours by weight percentage in temperature among the described step c.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2007100927120A CN101390562B (en) | 2007-09-18 | 2007-09-18 | Southernwood dregs biology protein and preparation method thereof |
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| CN2007100927120A CN101390562B (en) | 2007-09-18 | 2007-09-18 | Southernwood dregs biology protein and preparation method thereof |
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| CN101390562A true CN101390562A (en) | 2009-03-25 |
| CN101390562B CN101390562B (en) | 2011-11-02 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511650A (en) * | 2011-12-07 | 2012-06-27 | 河南科技大学 | Method for preparing protein feed by using Jerusalem artichoke residues |
| CN102920749A (en) * | 2012-11-05 | 2013-02-13 | 广西仙草堂制药有限责任公司 | Method for preparing fully-effective ganoderma lucidum health care product |
| CN103145509A (en) * | 2013-04-11 | 2013-06-12 | 曾品涛 | Edible fungus culture medium using sweet wormwood waste residue as main material and preparation method of edible fungus culture medium |
| CN103540536A (en) * | 2013-10-21 | 2014-01-29 | 甘肃省农业科学院土壤肥料与节水农业研究所 | Protein-producing microbial agent as well as preparation method and application thereof |
| CN111802511A (en) * | 2020-07-22 | 2020-10-23 | 中国中医科学院中药研究所 | Functional traditional Chinese medicine residue feed additive, preparation method and application |
-
2007
- 2007-09-18 CN CN2007100927120A patent/CN101390562B/en not_active Expired - Fee Related
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102511650A (en) * | 2011-12-07 | 2012-06-27 | 河南科技大学 | Method for preparing protein feed by using Jerusalem artichoke residues |
| CN102920749A (en) * | 2012-11-05 | 2013-02-13 | 广西仙草堂制药有限责任公司 | Method for preparing fully-effective ganoderma lucidum health care product |
| CN103145509A (en) * | 2013-04-11 | 2013-06-12 | 曾品涛 | Edible fungus culture medium using sweet wormwood waste residue as main material and preparation method of edible fungus culture medium |
| CN103540536A (en) * | 2013-10-21 | 2014-01-29 | 甘肃省农业科学院土壤肥料与节水农业研究所 | Protein-producing microbial agent as well as preparation method and application thereof |
| CN103540536B (en) * | 2013-10-21 | 2016-06-08 | 甘肃省农业科学院土壤肥料与节水农业研究所 | One is laid eggs white microbiobacterial agent and its preparation method and application |
| CN111802511A (en) * | 2020-07-22 | 2020-10-23 | 中国中医科学院中药研究所 | Functional traditional Chinese medicine residue feed additive, preparation method and application |
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| Publication number | Publication date |
|---|---|
| CN101390562B (en) | 2011-11-02 |
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Granted publication date: 20111102 Termination date: 20120918 |