CN101385790B - Intestine moisturizing soft capsules and preparation method and use thereof - Google Patents
Intestine moisturizing soft capsules and preparation method and use thereof Download PDFInfo
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Abstract
The invention discloses an intestine-moistening soft capsule which consists of liquid medicine and a capsule shell, wherein, the liquid medicine is made from a traditional Chinese medicine extract, a solvent, a stabilizer and a wetting agent, and the traditional Chinese medicine extract is extracted from 1-4 parts of peach kernel by weight, 0.5-2 parts of notopterygium root by weight, 0.5-2 parts of rhubarb by weight, 0.5-2 parts of angelica by weight and 1-4 parts of fructus cannabis by weight; and the capsule shell is made from 1 part of gelatin by weight, 0.3-0.5 part of glycerin by weight and 0.8-1.3 parts of water by weight. The invention also discloses a preparation method and the application thereof.
Description
Technical field
The present invention relates to the field of Chinese medicines, be specifically related to a kind of Chinese medicine intestine moistening soft capsule, method for preparing and purposes.
Background technology
Constipation is a kind of disease that people are the most often taken place.Have 50% people once to suffer from constipation according to estimates, cause the factor of constipation to have a lot, as along with growth in the living standard, the surfeit delicious food is given birth to heat, stops in the expectorant food, and the heat-transformation impairment of body fluid loses intestinal and moistens, and can cause constipation; Rhythm of life is accelerated, and operating pressure is excessive, and hypertonicity, fatigue make depression and stagnation of QI, and large intestine conduction dereliction of duty stops to cause constipation or the like in the waste matter.And these factors all are that modern life institute is inevitable.Constipation one disease is prone to treatment, but if lose control, wrong treatment can make constipation increase the weight of, pathogenic heat go into network become sick he, but like the with the passing of time hyperamization stasis of blood of the stagnation of QI, stasis of blood resistance meridians, injury of blood collaterals by heat hemorrhoid occur, has blood in stool, anal fissure.Because feces is long-time retention in intestinal; Fermentation corrupt back a large amount of harmful toxin of generation such as lactic acid, phenol, ammonia, hydrogen sulfide, indole, histamine etc.; A series of self poisoning condition of illness such as dizzy, weak, loss of appetite, abdominal part distension, susceptible to lose temper due to restlessness can be made us, also rectal cancer can be caused.Suffer from the constipation patient of coronary heart disease if defecation firmly can cause myocardial ischemia, the lighter's cardiopalmus, breathe hard, weight person even can die suddenly.The hypertensive can make hypertension, causes cerebral ischemia, dizziness, feeling of fullness in the head, seriously can cause cerebral hemorrhage.The old people can firmly send out apoplexy, angina pectoris, hernia even sudden death because of weak and sickly when constipation, constipation also is the one of the main reasons that causes alzheimer disease.So person " constipation is healthy formidable enemy " is arranged, answers active treatment for constipation.Therefore, develop treatment constipation medicine and will bring huge social benefit and economic benefit.
Summary of the invention
Main purpose of the present invention is to provide a kind of Chinese medicine intestine moistening soft capsule.
Another purpose of the present invention is to provide this above-mentioned Chinese medicine intestine moistening preparation of soft capsule method.
Still a further object of the present invention is to provide the purposes of this above-mentioned Chinese medicine intestine moistening soft capsule.
A kind of Chinese medicine intestine moistening of the present invention soft capsule is made up of medicinal liquid and softgel shell two parts.Wherein, medicinal liquid is made up of Chinese medicine extract, solvent, stabilizing agent and wetting agent; Softgel shell is made up of gelatin, G & W, and its weight proportion is a gelatin: glycerol: water=1: 0.3-0.5: 0.8-1.3 can add an amount of antibacterial in addition.
Solvent in the medicinal liquid according to the invention is a soybean oil.
Stabilizing agent in the medicinal liquid according to the invention can be one or more in Cera Flava, Petiolus Trachycarpi oil, the HPO, is preferably Cera Flava, Petiolus Trachycarpi oil and hydrogenated palm oil mixture.
Wetting agent in the medicinal liquid according to the invention is a soybean phospholipid.
In the medicinal liquid according to the invention, Chinese medicine extract is prepared from through following method:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 1～4 weight portion, Rhizoma Et Radix Notopterygii 0.5～2 weight portion, Radix Et Rhizoma Rhei 0.5～2 weight portion, Radix Angelicae Sinensis 0.5～2 weight portion, Fructus Cannabis 1～4 weight portion;
B. get Semen Persicae, Fructus Cannabis, 70%～90% alcohol reflux 1～3 time, each 1～3 hour, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, 50～70% ethanol percolation is collected liquid, and decompression recycling ethanol is ground into fine powder to doing, and gets the Radix Et Rhizoma Rhei dry extract, and is subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct 1 time again, and decocting time is 0.5～1.5 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
In the said extracted process, among the step a, the weight ratio of Chinese crude drug is preferably: Semen Persicae 2 weight portions, Rhizoma Et Radix Notopterygii 1 weight portion, Radix Et Rhizoma Rhei 1 weight portion, Radix Angelicae Sinensis 1 weight portion, Fructus Cannabis 2 weight portions; Among the step b, Semen Persicae, Fructus Cannabis preferably adopt 80% alcohol reflux 2 times, each 2 hours; Among the step c, preferred 60% ethanol carries out percolation, in the operating process of percolation; Adopt cylindrical or conical percolator, after Radix Et Rhizoma Rhei was suitably pulverized, the moistening of adding ethanol was flooded the percolator of packing into after 5～7 hours and is carried out percolation; Medical material uniformity after in percolator, pulverizing; Get rid of the air in the medical material gap when adding solvent, quantity of solvent will exceed the medical material face, floods to carry out percolation after 1～3 hour; In the steps d, the medicinal residues after the distillation preferably decocted 1 hour.
Among the present invention, the weight/volume proportioning of each component is in the intestine moistening soft capsule medicinal liquid:
25～35 parts of Semen Persicae, Fructus Cannabis extracts
90～110 parts of Radix Et Rhizoma Rhei dry extracts
230～250 parts of Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powders
1 part of Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil
180～200 parts of stabilizing agents
830～850 parts of solvents
40～45 parts of wetting agent
Need to prove: in above-mentioned each composition divided, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil were volume metering units, like milliliter (ml) or liter (L) etc.; Other composition is mass unit, like gram (g) or kilogram (kg) etc.Among the present invention, the proportioning of volume and quality is meant the volatile oil of above-mentioned certain volume and the ratio of other mass component, like ml/g or L/kg etc.Can find out that the former molecule and denominator enlarge 1000 times simultaneously and be the latter, its ratio value can not change.
Preferably, among the present invention, the weight/volume proportioning of each component is in the intestine moistening soft capsule medicinal liquid:
31 parts of Semen Persicae, Fructus Cannabis extracts
102 parts of Radix Et Rhizoma Rhei dry extracts
244 parts of Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powders
1 part of Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil
189 parts of stabilizing agents
843 parts of solvents
43 parts of wetting agent
Likewise, in above-mentioned each composition divided, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil were volume metering units, like milliliter (ml) or liter (L) etc.; Other composition is mass unit, like gram (g) or kilogram (kg) etc.Among the present invention, the proportioning of volume and quality is meant the volatile oil of above-mentioned certain volume and the ratio of other mass component, like ml/g or L/kg etc.Can find out that the former molecule and denominator enlarge 1000 times simultaneously and be the latter, its ratio value can not change.
Active component is fixed in each soft capsule.Wherein, to be equivalent to the crude drug amount be 0.5～1g/ grain to Chinese medicine extract.
In the prescription of softgel shell, the proportioning of gelatin, G & W is difficult to grasp.The amount of glycerol is too many, and softgel shell is softer, than being easier to extrusion; The amount of glycerol very little, softgel shell is harder, crisp, influences capsular quality stability and time.Among the present invention, the gelatin of softgel shell, the proportioning of glycerol are carried out deep experimental study, finally obtained comparatively ideal weight proportion scope.
Compared with prior art, chrysophanic acid content is high in the Chinese medicine intestine moistening soft capsule of the present invention.
The invention provides said Chinese medicine intestine moistening soft capsule preparation method, comprise Chinese medicine extraction processed Chinese medicine extract after, in solvent, process suspension with stabilizing agent, wetting agent mixed dissolution, be pressed into soft capsule, drying promptly gets soft capsule of the present invention.Wherein, softgel shell is made up of gelatin, G & W, and its weight proportion is a gelatin: glycerol: water=1: 0.3-0.5: 0.8-1.3 also has an amount of antibacterial in addition; Solvent in the said medicinal liquid is a soybean oil.Stabilizing agent in the medicinal liquid according to the invention can be one or more in Cera Flava, Petiolus Trachycarpi oil, the HPO, is preferably the mixture of Cera Flava, Petiolus Trachycarpi oil and HPO.Wetting agent in the said medicinal liquid is a soybean phospholipid.
Chinese medicine extract is prepared from through following method:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 1～4 weight portion, Rhizoma Et Radix Notopterygii 0.5～2 weight portion, Radix Et Rhizoma Rhei 0.5～2 weight portion, Radix Angelicae Sinensis 0.5～2 weight portion, Fructus Cannabis 1～4 weight portion;
B. get Semen Persicae, Fructus Cannabis, 70%～90% alcohol reflux 1～3 time, each 1～3 hour, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, 50～70% ethanol percolation is collected liquid, and decompression recycling ethanol is ground into fine powder to doing, and gets the Radix Et Rhizoma Rhei dry extract, and is subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct 1 time again, and decocting time is 0.5～1.5 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
In the said extracted process, among the step a, the weight ratio of Chinese crude drug is preferably: Semen Persicae 2 weight portions, Rhizoma Et Radix Notopterygii 1 weight portion, Radix Et Rhizoma Rhei 1 weight portion, Radix Angelicae Sinensis 1 weight portion, Fructus Cannabis 2 weight portions; Among the step b, Semen Persicae, Fructus Cannabis preferably adopt 80% alcohol reflux 2 times, each 2 hours; Among the step c, preferred 60% alcohol carries out percolation, in the operating process of percolation; Adopt cylindrical or conical percolator, after Radix Et Rhizoma Rhei was suitably pulverized, the moistening of adding ethanol was flooded the percolator of packing into after 5～7 hours and is carried out percolation; Medical material uniformity after in percolator, pulverizing; Get rid of the air in the medical material gap when adding solvent, quantity of solvent will exceed the medical material face, floods to carry out percolation after 1～3 hour; In the steps d, the medicinal residues after the distillation preferably decocted 1 hour.
Among the present invention, the weight/volume proportioning of each component is in the intestine moistening soft capsule medicinal liquid:
25～35 parts of Semen Persicae, Fructus Cannabis extracts
90～110 parts of Radix Et Rhizoma Rhei dry extracts
230～250 parts of Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powders
1 part of Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil
180～200 parts of stabilizing agents
830～850 parts of solvents
40～45 parts of wetting agent
Need to prove: in above-mentioned each composition divided, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil were volume metering units, like milliliter (ml) or liter (L) etc.; Other composition is mass unit, like gram (g) or kilogram (kg) etc.Among the present invention, the proportioning of volume and quality is meant the volatile oil of above-mentioned certain volume and the ratio of other mass component, like ml/g or L/kg etc.Can find out that the former molecule and denominator enlarge 1000 times simultaneously and be the latter, its ratio value can not change.
Preferably, among the present invention, the weight proportion of each component is in the intestine moistening soft capsule medicinal liquid:
31 parts of Semen Persicae, Fructus Cannabis extracts
102 parts of Radix Et Rhizoma Rhei dry extracts
244 parts of Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powders
1 part of Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil
189 parts of stabilizing agents
843 parts of solvents
43 parts of wetting agent
Likewise, in above-mentioned each composition divided, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil were volume metering units, like milliliter (ml) or liter (L) etc.; Other composition is mass unit, like gram (g) or kilogram (kg) etc.Among the present invention, the proportioning of volume and quality is meant the volatile oil of above-mentioned certain volume and the ratio of other mass component, like ml/g or L/kg etc.Can find out that the former molecule and denominator enlarge 1000 times simultaneously and be the latter, its ratio value can not change.
The present invention also provides above-mentioned intestine moistening soft capsule to have the effect of good loosening bowel to relieve constipation.
Chrysophanic acid content is high in the Chinese medicine intestine moistening soft capsule of the present invention.Can obviously promote the mice intestinal to advance, make defecation time in advance, loose stool quantity and loose stool rate obviously increase.Same dosed administration also has tangible progradation to the enterokinesia of mice dryness accumulated in the stomach and intestine type constipation.
Chrysophanic acid Study on content in the Test Example 1 intestine moistening soft capsule of the present invention
Adopt HPLC, intestine moistening soft capsule of the present invention and commercially available pill for smoothing intestines are compared.The result shows that conjunction type chrysophanic acid content is significantly higher than the intestine moistening soft capsule in the soft capsule of the present invention.
1. instrument and reagent
1.1 instrument: Waters 2690 chromatograph of liquid; Waters Symmetry Shield C
184.6mm * 250mm, 5 μ m; Sartorius110 type electronic analytical balance.
1.2 reagent: chrysophanic acid reference substance (self-control), methanol (chromatographically pure), acetic acid (chemistry alcohol) and ultra-pure water.
1.3 sample: according to the method preparation of embodiment 1,2 and 3, lot number is 20050801,20050802,20050803 respectively for 3 batches of intestine moistening soft capsules of the present invention (calling in the following text: " soft capsule "); Pill for smoothing intestines is available from LanZhou FoCi Pharmacy Co., Ltd, and lot number is respectively: 20030403,2005118,2006033 and 200612.
2. method and result
2.1 chromatographic condition: Waters Symmetry Shield C
18Post (250mm * 4.6mm, 5 μ m), flow velocity: 1.0ml/min, detect wavelength: 398nm, column temperature: 30 ℃, mobile phase: methanol-water-acetic acid (70: 30: 1)
2.2 the preparation of reference substance solution: it is an amount of that precision takes by weighing the chrysophanic acid reference substance, adds methanol and process the solution that contains 10 μ g chrysophanic acids among every 1ml, promptly gets.
2.3 the preparation of need testing solution: get these article " soft capsule " content and pill for smoothing intestines 0.45g respectively, accurate claim surely, put in the conical flask, precision adds methanol 20ml, claims decide weight, and supersound process 1 hour is put coldly, supplies weight with methanol, shakes up, and filters, and gets subsequent filtrate.Precision is measured subsequent filtrate 2ml and is put in the 5ml measuring bottle, adds methanol to scale, shakes up, and promptly gets the chrysophanic acid need testing solution that dissociates.Precision is measured above-mentioned subsequent filtrate 2ml and is put in the 20ml tool plug test tube again, puts and boils off methanol in the water-bath, and residue adds water 1.5ml, and 40% liquor ferri trichloridi 0.5ml put in the boiling water bath heating 20 minutes; Add hydrochloric acid 0.2ml, shake up, heating is 30 minutes in boiling water bath, cooling, the precision 10ml that adds diethyl ether; Jolting is left standstill, and precision is measured ether layer 5ml, puts in the tool plug bottle; Fling to ether, the residue precision adds methanol 5ml makes dissolving, filters, and promptly gets total chrysophanic acid need testing solution.
2.4 algoscopy: accurate respectively reference substance solution, free chrysophanic acid and each 10 μ l of total chrysophanic acid need testing solution of drawing, inject chromatograph of liquid respectively, mensuration is calculated total chrysophanic acid and free chrysophanic acid difference is the conjunction type quantitative determination of Rhein.
2.5 result: conjunction type quantitative determination of Rhein in each sample, see table 1.
The conjunction type quantitative determination of Rhein relatively in table 1, each sample
| Project | Free chrysophanic acid (mg/g) | Total chrysophanic acid (mg/g) | Conjunction type chrysophanic acid (mg/g) |
| The kind sample 1 of Lanzhou Buddhist | ?0.55 | ?0.60 | ?0.04 |
| The kind sample 2 of Lanzhou Buddhist | ?0.14 | ?0.35 | ?0.11 |
| The kind sample 3 of Lanzhou Buddhist | ?0.56 | ?0.78 | ?0.22 |
| The kind sample 4 of Lanzhou Buddhist | ?0.14 | ?0.41 | ?0.27 |
| Intestine moistening soft capsule 1 | ?0.69 | ?2.15 | ?1.46 |
| Intestine moistening soft capsule 2 | ?0.74 | ?2.45 | ?1.71 |
| Intestine moistening soft capsule 3 | ?0.68 | ?2.25 | ?1.57 |
3. conclusion
These article loosening bowel to relieve constipation is used for the excess-heat constipation, and Radix Et Rhizoma Rhei is to rush down the main Chinese medicinal materials of heat clearing away effect down in the prescription.Effective ingredient conjunction type chrysophanic acid is an index to rush down down in this test, has investigated the quality of commercially available pill for smoothing intestines and intestine moistening soft capsule of the present invention.The result shows that conjunction type chrysophanic acid content is significantly higher than the intestine moistening soft capsule among the present invention's " soft capsule ".
Test Example 2 soft capsules of the present invention are to the effect research (mice intestinal propulsion trial) of mice loosening bowel to relieve constipation
Observe of the effect of intestine moistening soft capsule through mice intestinal propulsion trial to the mice loosening bowel to relieve constipation
1. animal
60 of Kunming mouses (body weight: 19-21g), male and female half and half, available from Shanghai Si Laike laboratory animal responsibility company limited, production licence number: SCXK (Shanghai) 2003-0003, occupancy permit number: SYXK (Shanghai) 2004-0015.
2. reagent
3 batches of intestine moistening soft capsules of the present invention (calling in the following text: " soft capsule ") are respectively according to the method preparation of embodiment 1,2 and 3; Lot number is 20050801,20050802,20050803, is mixed with suspension with 0.5%CMC: and 0.05 g crude drug/ml (1g crude drug/kg), 0.15g crude drug/ml (3g crude drug/kg), 0.45g crude drug/ml (the 9g crude drug/kg);
Positive control drug: available from LanZhou FoCi Pharmacy Co., Ltd, lot number is respectively the kind pill for smoothing intestines of Buddhist (calling in the following text: " the kind pill for smoothing intestines of Buddhist "): 20030403, be mixed with 0.05g crude drug/ml (suspension of 1g crude drug/kg) with 0.5%CMC; Tang Long pill for smoothing intestines (calling in the following text: " Tang Long pill for smoothing intestines ") is available from Taibao Pharmaceutical Co., Ltd., Lanzhou, lot number: 88060326, be mixed with 0.05g crude drug/ml (suspension of 1g crude drug/kg) with 0.5%CMC.
3. method
Get 60 of mices, be divided into 6 groups, every group 5 male 5 female.Divide into groups: blank group, the kind pill for smoothing intestines group of Buddhist (1g crude drug/kg), Tang Long pill for smoothing intestines group (1g crude drug/kg), intestine moistening soft capsule low dose group (dose groups in 1g crude drug/kg), the intestine moistening soft capsule (3g crude drug/kg), intestine moistening soft capsule high dose group (the 9g crude drug/kg), administration volume: 0.4ml/20g body weight.
All mice fasting are 24 hours before the test, freely drink water.Administration group per os filling stomach respectively gives relative medicine.The blank group gives equal-volume 0.5%CMC.Above medicine all contains 10% burnt black ink.After the administration 20 minutes, take off cervical vertebra and put to death mice, open the abdominal cavity immediately; Separate mesentery, the little intestinal tube of clip pylorus to ileocecus is put on the pallet; Gently pull into straight line, measure small intestinal length overall and carbon powder distance, calculate the carbon powder propelling percentage rate of each group by following formula at enteral propelling front end.The result carries out the t check.
4. result of the test and conclusion
The result sees table 2.
Table 2. intestine moistening soft capsule is to the influence of mouse small intestine progradation
Annotate:
*Compare with the blank group: p＜0.01.
The above results shows that basic, normal, high group of intestine moistening soft capsule and matched group relatively all can obviously promote the progradation of mice intestinal.Compared with prior art, the present invention adopts the Radix Et Rhizoma Rhei among percolation the other side to extract separately, has increased the conjunction type quantitative determination of Rhein, makes it increase the progradation of mice intestinal.
Test Example 3 intestine moistening soft capsules are to the influence of mice excess-heat constipation model
Bring out the mice constipation through diphenoxylate, observe of the effect of intestine moistening soft capsule the mice loosening bowel to relieve constipation.
1. animal
70 of Kunming mouses (body weight: 19-21g), male and female half and half, available from Shanghai Si Laike laboratory animal responsibility company limited, production licence number: SCXK (Shanghai) 2003-0003, occupancy permit number: SYXK (Shanghai) 2004-0015.
2. reagent
3 batches of intestine moistening soft capsules of the present invention (calling in the following text: " soft capsule ") are respectively according to the method preparation of embodiment 1,2 and 3; Lot number is 20050801,20050802,20050803, is mixed with suspension with 0.5%CMC: and 0.05g crude drug/ml (1g crude drug/kg), 0.15g crude drug/ml (3g crude drug/kg), 0.45g crude drug/ml (the 9g crude drug/kg);
Positive control drug: the kind pill for smoothing intestines of Buddhist (calling in the following text: " the kind pill for smoothing intestines of Buddhist ") is purchased white orchid state Fo Ci Pharmacy stock Co., Ltd, and lot number is respectively: 20030403; Tang Long pill for smoothing intestines (calling in the following text: " Tang Long pill for smoothing intestines ") is available from Taibao Pharmaceutical Co., Ltd., Lanzhou; Lot number: 88060326, be mixed with suspension with 0.5%CMC: 0.05g crude drug/ml (1g crude drug/kg), 0.15g crude drug/ml (3g crude drug/kg), 0.45g crude drug/ml (the 9g crude drug/kg).。
The modeling medicine: the diphenoxylate sheet, Kang Pu pharmaceutcal corporation, Ltd in Changzhou produces, lot number: 0608017, specification: 2.5mg/ preparation: the suspension that is mixed with 2.5mg/ml with distilled water.
3. method and result
3.1 method:
Get 70 of mices, be divided into 7 groups, every group 5 male 5 female.Divide into groups: blank group, model group, the kind pill for smoothing intestines group of Buddhist (1g crude drug/kg), Tang Long pill for smoothing intestines group (1g crude drug/kg), intestine moistening soft capsule low dose group (dose groups in 1g crude drug/kg), the intestine moistening soft capsule (3g crude drug/kg), intestine moistening soft capsule high dose group (the 9g crude drug/kg), administration volume: 0.4ml/20g body weight.
Each administration group administration every day 1 time, successive administration 3 days.Blank group and model group give equal-volume 0.5%CMC.Formal test fasting in preceding 24 hours is freely drunk water.Test the same day, except that the blank group, all the other 6 groups equal per os are irritated stomaches and are given 2.5mg/ml diphenoxylate aqueous suspension 0.4ml/20g body weight (50mg/kg).After 30 minutes, each administration group gives relative medicine respectively, and blank group and model group give equal-volume 0.5%CMC.All animals are put into independent little cage respectively, and shop filter paper was observed 12 hours continuously at the bottom of the cage, and the argol that writes down each treated animal is counted, loose stool is counted and do not have the animal of defecation, and result and model group relatively and are carried out sided t and checked.
3.2 result and conclusion
The result sees table 3.
Table 3. intestine moistening soft capsule brings out the influence of excess-heat mice with constipation defecation to diphenoxylate
Annotate:
*Compare with the blank group: p＜0.01
The above results shows: the high, medium and low dose groups of intestine moistening soft capsule can increase significantly all that argol that diphenoxylate brings out mice excess-heat constipation model is counted and loose stool is counted, and result and model group relatively have significant difference.Show that the intestine moistening soft capsule has tangible promotion defecation to the excess-heat mice with constipation.Compared with prior art, the present invention adopts the Radix Et Rhizoma Rhei among percolation the other side to extract separately, has increased the conjunction type quantitative determination of Rhein, has increased it and has promoted the effect of defecation.
The specific embodiment
Below further specify protection scope of the present invention through the specific embodiment, but following examples constitute restriction to the present invention never in any form.
Embodiment 1
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 214g, Rhizoma Et Radix Notopterygii 107g, Radix Et Rhizoma Rhei 107g, Radix Angelicae Sinensis 107g, Fructus Cannabis 214g;
B. get Semen Persicae, Fructus Cannabis, add 80% alcohol reflux 2 times, each 2 hours, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 60% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 10.3g; Radix Et Rhizoma Rhei dry extract 33.69g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 82.52g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml, Petiolus Trachycarpi oil 21.6g HPO 24g Cera Flava 16.8g soybean phospholipid 14.4g soybean oil 278.33g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 40g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Embodiment 2
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 214g, Rhizoma Et Radix Notopterygii 107g, Radix Et Rhizoma Rhei 107g, Radix Angelicae Sinensis 107g, Fructus Cannabis 214g;
B. get Semen Persicae, Fructus Cannabis, add 60% alcohol reflux 2 times, each 1 hour, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 60% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 10.3g; Radix Et Rhizoma Rhei dry extract 33.69g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 82.52g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml, Petiolus Trachycarpi oil 21.6g HPO 24g Cera Flava 16.8g soybean phospholipid 14.4g soybean oil 278.33g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 40g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Embodiment 3
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 214g, Rhizoma Et Radix Notopterygii 107g, Radix Et Rhizoma Rhei 107g, Radix Angelicae Sinensis 107g, Fructus Cannabis 214g;
B. get Semen Persicae, Fructus Cannabis, add 80% alcohol reflux 2 times, each 1.5 hours, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 60% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 10.3g; Radix Et Rhizoma Rhei dry extract 33.69g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 82.52g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml, Petiolus Trachycarpi oil 21.6g HPO 24g Cera Flava 16.8g soybean phospholipid 14.4g soybean oil 278.33g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 40g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Embodiment 4
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 321g, Rhizoma Et Radix Notopterygii 53.5g, Radix Et Rhizoma Rhei 53.5g, Radix Angelicae Sinensis 107g, Fructus Cannabis 214g;
B. get Semen Persicae, Fructus Cannabis, add 80% alcohol reflux 2 times, each 1.5 hours, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 70% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 10.3g; Radix Et Rhizoma Rhei dry extract 33.69g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 82.52g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml, Petiolus Trachycarpi oil 21.6g HPO 24g Cera Flava 16.8g soybean phospholipid 14.4g soybean oil 278.33g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 40g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Embodiment 5
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 107g, Rhizoma Et Radix Notopterygii 160.5g, Radix Et Rhizoma Rhei 160.5g, Radix Angelicae Sinensis 214g, Fructus Cannabis 107g;
B. get Semen Persicae, Fructus Cannabis, add 80% alcohol reflux 2 times, each 1.5 hours, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 70% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 10.3g; Radix Et Rhizoma Rhei dry extract 33.69g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 82.52g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml, Petiolus Trachycarpi oil 21.6g HPO 24g Cera Flava 16.8g soybean phospholipid 14.4g soybean oil 278.33g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 40g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Embodiment 6
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 321g, Rhizoma Et Radix Notopterygii 53.5g, Radix Et Rhizoma Rhei 53.5g, Radix Angelicae Sinensis 53.5g, Fructus Cannabis 267.5g;
B. get Semen Persicae, Fructus Cannabis, add 80% alcohol reflux 2 times, each 1.5 hours, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 70% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 10.3g; Radix Et Rhizoma Rhei dry extract 33.69g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 82.52g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml, Petiolus Trachycarpi oil 21.6g HPO 24g Cera Flava 16.8g soybean phospholipid 14.4g soybean oil 278.33g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 40g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Embodiment 7
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 321g, Rhizoma Et Radix Notopterygii 53.5g, Radix Et Rhizoma Rhei 53.5g, Radix Angelicae Sinensis 53.5g, Fructus Cannabis 267.5g;
B. get Semen Persicae, Fructus Cannabis, add 80% alcohol reflux 2 times, each 1.5 hours, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 70% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 10.3g; Radix Et Rhizoma Rhei dry extract 33.69g, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 82.52g, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml; HPO 62.4g soybean phospholipid 14.4g soybean oil 278.33g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 40g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Embodiment 8
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 321g, Rhizoma Et Radix Notopterygii 53.5g, Radix Et Rhizoma Rhei 53.5g, Radix Angelicae Sinensis 53.5g, Fructus Cannabis 267.5g;
B. get Semen Persicae, Fructus Cannabis, add 80% alcohol reflux 2 times, each 1.5 hours, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 55% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 10.3g, Radix Et Rhizoma Rhei dry extract 33.69g, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 82.52g, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml, Petiolus Trachycarpi oil 62.4g soybean phospholipid 14.4g soybean oil 278.33g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 40g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Embodiment 9
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 321g, Rhizoma Et Radix Notopterygii 53.5g, Radix Et Rhizoma Rhei 53.5g, Radix Angelicae Sinensis 53.5g, Fructus Cannabis 267.5g;
B. get Semen Persicae, Fructus Cannabis, add 80% alcohol reflux 2 times, each 1.5 hours, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 70% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 10.3g; Radix Et Rhizoma Rhei dry extract 33.69g, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 82.52g, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml; HPO 32.4g Cera Flava 30g soybean phospholipid 14.4g soybean oil 278.33g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 40g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Embodiment 10
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 321g, Rhizoma Et Radix Notopterygii 53.5g, Radix Et Rhizoma Rhei 53.5g, Radix Angelicae Sinensis 53.5g, Fructus Cannabis 267.5g;
B. get Semen Persicae, Fructus Cannabis, add 80% alcohol reflux 2 times, each 1.5 hours, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 50% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 10.3g; Radix Et Rhizoma Rhei dry extract 33.69g, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 82.52g, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml; Petiolus Trachycarpi oil 32.4g HPO 30g soybean phospholipid 14.4g soybean oil 278.33g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 40g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Embodiment 11
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 214g, Rhizoma Et Radix Notopterygii 107g, Radix Et Rhizoma Rhei 107g, Radix Angelicae Sinensis 107g, Fructus Cannabis 214g;
B. get Semen Persicae, Fructus Cannabis, add 80% alcohol reflux 2 times, each 2 hours, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 60% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 10.3g; Radix Et Rhizoma Rhei dry extract 33.69g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 82.52g; Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml, Petiolus Trachycarpi oil 21.6g HPO 24g Cera Flava 16.8g soybean phospholipid 14.4g soybean oil 278.33g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 24g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Embodiment 12
The preparation of Chinese medicine extract:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 214g, Rhizoma Et Radix Notopterygii 107g, Radix Et Rhizoma Rhei 107g, Radix Angelicae Sinensis 107g, Fructus Cannabis 214g;
B. get Semen Persicae, Fructus Cannabis, add 80% alcohol reflux 2 times, each 2 hours, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol with 65% flooded 24 hours as solvent, according to the speed of every kilogram of medical material per minute 2ml percolation slowly, collected liquid 1000ml, and decompression recycling ethanol is ground into fine powder to doing, the Radix Et Rhizoma Rhei dry extract, subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with steam distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct, and decocting time is 1 hour, filter, and merging filtrate, concentrating under reduced pressure, drying is ground into fine powder, gets Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, and is subsequent use.
Preparation of soft capsule
Get Semen Persicae, Fructus Cannabis extract 9.88g; Radix Et Rhizoma Rhei dry extract 31.33g, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 89.40g, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil 0.33ml; Petiolus Trachycarpi oil 21.6g HPO 24g Cera Flava 9g soybean phospholipid 12.7g soybean oil 285.20g is mixed with the suspension of soft capsule content.Get gelatin 80g, glycerol 24g, water 80g and prepare cyst membrane, add suspension and be pressed into soft capsule.
Claims (9)
1. intestine moistening soft capsule; Form by medicinal liquid and softgel shell two parts; Its herb liquid is made up of Chinese medicine extract, solvent, stabilizing agent and wetting agent; Chinese medicine extract is by by weight Semen Persicae 1-4 part, and Rhizoma Et Radix Notopterygii 0.5-2 part, Radix Et Rhizoma Rhei 0.5-2 part, Radix Angelicae Sinensis 0.5-2 part, Fructus Cannabis 1-4 part are extracted gained; Softgel shell is made up of by weight 1 part in gelatin, glycerol 0.3-0.5 part and water 0.8-1.3 part; Wherein said Chinese medicine extract is prepared by the following step: (a) get raw medicinal material according to above-mentioned weight ratio, (b) get Semen Persicae, Fructus Cannabis, 70-90% alcohol reflux 1-3 time; Each 1-3 hour, filter merging filtrate; Decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract; (c) get Radix Et Rhizoma Rhei, the ethanol percolation of 50-70% will be collected the liquid decompression recycling ethanol to doing, and be ground into fine powder, get the Radix Et Rhizoma Rhei dry extract; (d) Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with vapor distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct 1 time again, and decocting time is 0.5-1.5 hour, filters, and merging filtrate, is ground into fine powder at concentrating under reduced pressure, drying, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder.
2. soft capsule according to claim 1, wherein said stabilizing agent are one or more in Cera Flava, Petiolus Trachycarpi oil, the HPO.
3. soft capsule according to claim 1, wherein said wetting agent are soybean phospholipid.
4. soft capsule according to claim 1, wherein said solvent are soybean oil.
5. soft capsule according to claim 1, wherein among the step a, weight ratio Semen Persicae 2 weight portions of Chinese crude drug, Rhizoma Et Radix Notopterygii 1 weight portion, Radix Et Rhizoma Rhei 1 weight portion, Radix Angelicae Sinensis 1 weight portion, Fructus Cannabis 2 weight portions.
6. soft capsule according to claim 1, wherein among the step b, Semen Persicae, Fructus Cannabis adopt 80% alcohol reflux 2 times, each 2 hours.
7. soft capsule according to claim 1 wherein among the step c, adopts 60% ethanol to carry out percolation.
8. soft capsule according to claim 1, wherein in the steps d, the medicinal residues after the distillation decocted 1 hour.
9. an intestine moistening preparation of soft capsule method comprises the steps:
A. get raw medicinal material according to the following weight ratio: Semen Persicae 1-4 weight portion, Rhizoma Et Radix Notopterygii 0.5-2 weight portion, Radix Et Rhizoma Rhei 0.5-2 weight portion, Radix Angelicae Sinensis 0.5-2 weight portion, Fructus Cannabis 1-4 weight portion;
B. get Semen Persicae, Fructus Cannabis, 70-90% alcohol reflux 1-3 time, each 1-3 hour, filter, merging filtrate, decompression recycling ethanol gets Semen Persicae, Fructus Cannabis extract, and is subsequent use;
C. get Radix Et Rhizoma Rhei, the ethanol percolation of 50-70% will be collected the liquid decompression recycling ethanol to doing, and be ground into fine powder, get the Radix Et Rhizoma Rhei dry extract, and will be subsequent use;
D. Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii are extracted volatile oil with vapor distillation, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil are subsequent use; Decocting liquid after the distillation filters, and medicinal residues add water and decoct 1 time again, and decocting time is 0.5-1.5 hour, filters, and merging filtrate, is ground into fine powder at concentrating under reduced pressure, drying, Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder, subsequent use;
E. the above-mentioned extract that obtains and stabilizing agent, solvent and wetting agent are mixed and made into suspension according to certain weight ratio, are pressed into soft capsule, drying promptly gets soft capsule;
Wherein, the weight/volume proportioning of each component is in the medicinal liquid:
Semen Persicae, Fructus Cannabis extract 25-35 part
Radix Et Rhizoma Rhei dry extract 90-110 part
Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii extract powder 230-250 part
1 part of Radix Angelicae Sinensis, Rhizoma Et Radix Notopterygii volatile oil
Stabilizing agent 180-200 part
Solvent 830-850 part
Wetting agent 40-45 part.
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| CN104738629A (en) * | 2015-04-10 | 2015-07-01 | 山东安然纳米实业发展有限公司 | Health food with functions of lubricating intestines and relaxing bowels |
| CN108524631A (en) * | 2018-03-12 | 2018-09-14 | 安徽海神寿春药业有限公司 | It is a kind of mix peach kernel, fructus cannabis ease constipation extract powder production method |
| CN111956686A (en) * | 2020-08-20 | 2020-11-20 | 武汉自然萃创新科技有限公司 | Honey enema and preparation method thereof |
| CN113892642B (en) * | 2021-08-25 | 2024-03-08 | 仙乐健康科技股份有限公司 | Probiotic composition for relaxing bowel |
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| CN1742838A (en) * | 2005-09-23 | 2006-03-08 | 北京阜康仁生物制药科技有限公司 | Xishengteng external-use medicine |
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| CN1742838A (en) * | 2005-09-23 | 2006-03-08 | 北京阜康仁生物制药科技有限公司 | Xishengteng external-use medicine |
Non-Patent Citations (2)
| Title |
|---|
| 中华人民共和国卫生部药典委员会.润肠丸（浓缩丸）.《中华人民共和国卫生部药品标准中药成方制剂第十一册》.1996,157. * |
| 中华人民共和国卫生部药典委员会.润肠丸.《中华人民共和国卫生部药品标准中药成方制剂第五册》.1991,146. * |
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