CN106491719A - A kind of extracting method of leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract and its application - Google Patents
A kind of extracting method of leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract and its application Download PDFInfo
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- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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Abstract
The invention belongs to the field of Chinese medicines, more particularly to a kind of new extracting method of Broussonetia papyrifera heaf total phenolic acid and its application.Described extracting method is specially：The leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. of appropriate natural drying is taken, powder is ground into；Leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. powder is put into supercritical CO2In extraction kettle, first with 85% ethanol as entrainer, extraction time is 1.5 h, and extracting pressure is 50.0MPa, and extraction temperature is 50 DEG C, CO2Flow is 10 kg h‑1, separating pressure is 5.8 MPa, and separation temperature is 40 DEG C；The ethanol of gained after extraction, through concentrating under reduced pressure, drying under reduced pressure, obtains leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract.The leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract that extracting method of the present invention is obtained, method are easy, low cost, and purity is high, is that its medicine for being developed into anti-parkinson is laid a good foundation.
Description
Technical field
The invention belongs to the field of Chinese medicines, more particularly to a kind of new extracting method of Broussonetia papyrifera heaf total phenolic acid and its application.
Background technology
Broussonetia papyrifera（Broussonetia papyrifera）For Moraceae Broussonetia papyrifera platymiscium, China the Yellow River, the Changjiang river, pearl is distributed in
River basin, is also distributed in states such as Japan, Vietnam, India, resource very abundant.The mature fruit of Broussonetia papyrifera, referred to as " Fructus Broussonetiae ",
Record in going through version《Chinese Pharmacopoeia》, it is common Chinese medicine, have functions that kidney tonifying clearing liver, improving eyesight diuresis.Leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. is also called Folium Broussonetiae,
Sweet in the mouth cool in nature, nontoxic, can clearing away heat-damp and promoting diuresis, cooling blood for hemostasis, parasite killing detoxify, can be used for treat haematemesis, epistaxis, metrorrhagia, traumatic hemorrhage,
The disease such as edema, hernia, dysentery and tinea skin ulcer, climacteric syndrome, antiviral and prostatitis.
From the eighties in 20th century, people start the chemistry to Broussonetia papyrifera and pharmacology is studied.For example：From the whole of Broussonetia papyrifera
Isolated multiple flavone compounds in strain plant（Lee D, Bhat K, Fong H et al. Aromatase
Inhibitors from Broussonetia papyrifera [J] .J. Nat. Prod, 2001,64 (10): 1286-
1293）；Ethanol extraction to leaf of Broussonetia papyrifera (L.) L.Her.ex Vent.（BPAE）With total flavonoid glycoside（BPF）The effect of rabbit and Isolated Atrium of Guinea Pigs is carried out
Research（Gao Yunsheng, Qiu Yufang, Gao Ling etc. leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. alcohol extracting thing and inhibitory action [J] of the flavonoid glycoside to isolated atria.
Chinese Pharmacological Bulletin, 1988,4 (2)：122-123）, it is found that BPAE and BPF has the work for suppressing that rabbit and guinea-pig atrial shrink
With, and the potency of BPF be much larger than BPAE, it was confirmed that BPF be Broussonetia papyrifera suppress atrial systole principle active component, leaf of Broussonetia papyrifera (L.) L.Her.ex Vent.
There is the effect of similar calcium antagonist.In leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. in addition to flavone, also contain phenolic hydroxyl group material, such as lignanoid etc. containing other,
These materials are referred to as phenolic acid（Ran Xiao-Ku, Wang Xiao-Tong,Liu Pei-Pei, Chi Yu-Xin,
Wang Bo-Jia, Dou De-Qiang, Kang Ting-Guo, Xiong Wei.Cytotoxic constituents
from the leaves of Broussonetia papyrifera.Chinese Journal of Natural
Medicines 2013,11(3):269-273.）.In the research to leaf of Broussonetia papyrifera (L.) L.Her.ex Vent., it is found that there is total phenolics preferable anticancer to make
With（A kind of leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extracts of CN102319291B and its application in cancer therapy drug is prepared）, non-goodbye has other
Medicinal application research relevant report.
Parkinson disease（PD）It is the nervus centraliss with substantia nigra of midbrain dopaminergic neuron progressive death as pathological characters
System degenerative disease.At present treatment meanss are mainly based on Western medicine, but Western medicine only improves symptom, it is impossible to control its process, long
Phase application curative effect weaken and side effect increase, and Chinese medicine be by adjust body integral status and work, mild in medicine property and, poison
Small side effects.Chinese medicine is long-term in aspect achievements in research such as protection neurocyte, inhibited oxidation stress, anti-excitatory toxicities
Treatment parkinson disease, effective control parkinson disease process haves laid a good foundation, in recent years, treatment by Chinese herbs parkinson disease into
For one of clinical essential therapeutic arsenals.Also, if patient takes merely Chinese medicine energy effective control Early Parkinson's disease symptom, both
The toxic and side effects of Western medicine are avoided that, the compliance of patient's medication can be greatly enhanced again；Therefore, anti-Parkinson is found from Chinese medicine
Effective site, and carry out new drug development, it appears particularly important.
Content of the invention
For the problems referred to above, the present invention provides a kind of new process for extracting of leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract, and the extraction
Thing is used for preparing the new application in antiparkinsonism drug, and the medicine is with strong points, has no toxic side effect.
For achieving the above object, the present invention provides a kind of new process for extracting of leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract, specifically includes
Following steps：The leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. of appropriate natural drying is taken, powder is ground into；Leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. powder is put into supercritical CO2In extraction kettle,
First with 85% ethanol as entrainer, extraction time is 1.5 h, and extracting pressure is 40.0 MPa, and extraction temperature is 50 DEG C, CO2
Flow is 10 kg h-1, separating pressure is 5.8 MPa, and separation temperature is 40 DEG C；The ethanol of gained after extraction, through decompression
Concentration, drying under reduced pressure obtain leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract.
In described leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract, total phenols acid content is more than 50%, cosmos in heaf total phenolic acid extract
Glycosides content 3-7%, 3,5,4 '-trihydroxy-Diphenethyl -3-O- β-D-Glucose glycosides (3,5,4 '-trihydroxy-
Bibenzyl-3-O- β-D-glucopyranside) it is 1-3%.
Described leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract is used for preparing anti-Parkinson medicine.
Beneficial effects of the present invention.
The present invention obtains the total phenolics of high level, leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract from leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. using supercritical fluid
Total phenolics assay the content of total phenolics is measured using the colorimetry of following document reports：Shi Yuyuan, He Fan, Lee
Sparkling, Dou Deqiang, Kang Tingguo, Dong Feng. the assay of total phenolics in different sources Ya Gongye. Chinese Chinese medicine academic periodical, 2010,
28（7）：1475-1477.We have found that in anti-parkinson Effective Component of Chinese Medicine screening process Broussonetia papyrifera heaf total phenolic acid has
The effect of good anti-parkinson, evaluates through in vivo test and serum pharmacological and find Broussonetia papyrifera heaf total phenolic acid with preferably
Anti-Parkinson is acted on.The leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract that extracting method of the present invention is obtained, method are easy, low cost, and purity is high,
Lay a good foundation for its medicine for being developed into anti-parkinson.
Description of the drawings
Fig. 1 leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. sample high effective liquid chromatography for measuring chromatograms；Wherein I is cosmosiin, II is 3,5,4 '-
Trihydroxy-Diphenethyl -3-O- β-D-Glucose glycosides.
Specific embodiment
With reference to specific embodiment, the present invention is further illustrated.
Embodiment 1.
Prepare leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract：Leaf of Broussonetia papyrifera (L.) L.Her.ex Vent.（Including petiole）, after natural drying, it is ground into the powder of 20 mesh；Claim
200 grams of leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. powder is taken, supercritical CO is put into2In extraction kettle（INSTRUMENT MODEL：HSCE-50-1, Dalian Zhuo Er high-tech are limited
Company produces）；Extracted according to following condition：85% ethanol（The volume ratio of second alcohol and water）For entrainer, extraction time is
1.5 h, extracting pressure are 50.0 MPa, and extraction temperature is 50 DEG C；CO2Flow is 10 kg h-1, separating pressure is 5.8
MPa, separation temperature are 40 DEG C；The ethanol of gained after extraction, obtains extractum through concentrating under reduced pressure, further through drying under reduced pressure, obtains structure
Leaveves total phenolic acid extract.
Total phenolic acid extract weight is 16.2g, measures total phenolics for 8.93g, accounts for the dried total phenolic acid extract
Percentage by weight be 55.1%, account for leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. raw medicinal material gross weight percentage ratio be 4.5%；In total phenolic acid extract, big Persian
Echinacoside content is 5.21%, 3,5, and 4 '-trihydroxy-Diphenethyl -3-O- β-D-Glucose glycosides content is 1.78%.
1. the assay method of total phenolics, specific operation process are as follows.
Precision weighs the total phenolic acid extract of 10mg in 10 milliliters of volumetric flasks, plus methanol ultrasonic dissolution, constant volume, prepares and supplies
Test sample solution.Precision measures need testing solution 1ml, in 25ml measuring bottles, plus methanol 5ml, plus 0.3% sodium lauryl sulphate 2ml
Afterwards, then plus 0.6% FeCl3.6H2O：0.9%K3[Fe(CN)6] it is 1：1（Volume ratio) mixed solution 2ml, place in the dark
5min, plus 0.1mol/l HCl solutions are to scale, place 20min in the dark, with developer as blank, with chlorogenic acid be to product,
Mensuration absorbance at the 746nm.Calibration curve equation Y=145.2X+0.0303 (r=0.9997, the range of linearity according to chlorogenic acid
0.002 -0.005 mg/mL.Wherein Y is absorbance, and X is reference substance solution concentration (mg/mL)), carry out quantitative analyses.
2. the measure of cosmosiin and 3,5,4 '-trihydroxy-Diphenethyl -3-O- β-D-Glucose glycosides content is adopted
High performance liquid chromatography, chromatographic condition are as follows：Instrument is 1100 type high performance liquid chromatographs of Agilent（U.S.'s Agilent is public
Department）, chromatographic column：Kromasil C18 100A (5μm, 250×4.6mm)；With methanol as mobile phase A, with 0.5% phosphoric acid water
For Mobile phase B, gradient elution：It is within 0 minute 35%A, is within 25 minutes 45%A, Detection wavelength is 265 nm；Column temperature is 30 DEG C；Sample introduction
Measure as 10 μ L；Run time 25min, measurement result are shown in Fig. 1.
Prepared by need testing solution：Precision weighs the total phenolic acid extract of 10mg in 10 milliliters of volumetric flasks, plus methanol ultrasound
Dissolving, constant volume, prepare need testing solution.With 0.45 μm of membrane filtration before sample introduction, standby.
The preparation of standard solution：Precision weigh cosmosiin and 3,5,4 '-trihydroxy-Diphenethyl -3-O- β -
D-Glucose glycosides sample（Self-control, purity are more than 98%）5mg, methanol constant volume is in 20ml volumetric flasks.
3. leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract anti-Parkinson Effect study.
3.1. experiment material.
C57BL/6 mices are purchased from Dalian Medical Univ's animal experimental center, and leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract used is employing
Prepared by said method.
3.2. animal packet.
50 C57BL/6 mices are randomly divided into 4 groups：Blank group, model group, positive drug group（Benserazide piece（U.S. many
Fragrant plant）, Shanghai company limited of Roche Group）With total phenolics high and low dose group, 10 per group.
3.3. modeling and medication.
Model group, positive drug group, Broussonetia papyrifera heaf total phenolic acid height and low dose group subcutaneous injection MPTP（Sigma companies are purchased from,
Lot number：1001054803）25 mg/kg, once a day, continuous 4 days.Blank group is replaced with lumbar injection normal saline
MPTP, once a day, continuous 4 days.5th day starts, and continues subcutaneous injection MPTP, while Broussonetia papyrifera heaf total phenolic acid high and low dose group
150mg/kg, 75mg/kg is administered respectively, is administered once a day, successive administration 9 days.Positive drug group gives many of 50mg/kg daily
Bar silk hydrazine piece, blank group and model group give the normal saline of equivalent daily.Carry out Animal Behavior Science experiment daily to examine to mice
Look into.
3.4. behavioristicss' examination.
3.4.1 experiment is hung.
Purpose is detection C57BL/6 mouse limb motor coordination situations.Give the 7th, 8 and 9 days after Broussonetia papyrifera heaf total phenolic acid,
Test mice is hung in a horizontal wire, the diameter of wire is about 0.2mm.Electric wire is caught to start timing from the double fore paws of mice,
Mice drops, and timing terminates.6 points of the note of more than 25 seconds；The 20-25 seconds remember 5 points；The 15-20 seconds remember 4 points；The 10-15 seconds remember 3 points；5-
10s remembers 2 points；0-5s remembers 1 point.Scoring event, and take statistics credit analysis are finally calculated.Score value is higher, and mice catches electric wire more firm
Gu；Illustrate that mouse limb motor coordination situation is better, conversely, then explanation mice tremble, myotonia situations such as serious.Experimental result
It is shown in Table 1.
Table 1 hangs experiment scores（±s）.
Note：△ is compared with blank group, variant（p<0.05）；△ △ are compared with blank group, significant difference（p<0.01）；▲
Compare with model group, variant（p<0.05）, ▲ ▲ is compared with model group, and there were significant differences（p<0.01）.
3.4.2 Grasping clubglass test.
Purpose is detection C57BL/6 mouse limb motor coordination situations.In giving the 7th, 8 and 9 days of Broussonetia papyrifera heaf total phenolic acid,
The cork ball of a diameter of 2.5 cm is fixed on long 50 cm, the rod top of 1 cm of diameter, be wrapped with gauze on rod in case
Skid, then tested mice is put on bead, and records the time that mice has been climbed required for pole.Then remember by following standard
Point：6 points of the note of above-mentioned action is completed in the 0-3 seconds；5 points of the note completed in the 3-6 seconds；4 points of the note completed in the 6-9 seconds；The 9-12 seconds
3 points of the note for inside completing；2 points of the note completed in the 12-15 seconds；1 point of the note of more than 15 seconds.Scoring event is finally calculated, and is taken statistics
Credit is analysed.Score value is higher, and the time that mice has been climbed used by the whole process of pole is fewer, illustrates that mouse limb motor coordination situation is got over
Good, also explanation mice is more and more skilled to Grasping clubglass test, and experimental result is shown in Table 2.
2 Grasping clubglass test scores of table（±s）.
Note：△ is compared with blank group, variant（p<0.05）；△ △ are compared with blank group, significant difference（p<0.01）；▲
Compare with model group, variant（p<0.05）, ▲ ▲ is compared with model group, and there were significant differences（p<0.01）.
3.4.3 swimming test.
Purpose is detection C57BL/6 mouse limb motor coordination situations.In giving the 7th, the 8 of leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract
With 9 days, test mice is put in the water tank of 20 cm × 30cm × 20 cm specifications, water temperature be 22 DEG C -25 DEG C.
The time of mice floating in record 1min.Standards of grading are as follows：In 1min, 6 points of note of the flotation time in 10s；In 20s
5 points of note；4 points of note in 30s；3 points of note in 40s；2 points of note in 50s；1 point of the note beyond 50s.Score feelings are finally calculated
Condition, and the credit analysis that takes statistics.Score value is higher, and the time of mice swimming is more；Illustrate that mouse limb motor coordination situation is better, instead
It, then explanation mice tremble, myotonia situations such as serious, the results are shown in Table 3.
3 swimming test scores of table（±s）.
Note：△ is compared with blank group, variant（p<0.05）；△ △ are compared with blank group, significant difference（p<0.01）；▲
Compare with model group, variant（p<0.05）；▲ ▲ compare with model group, there were significant differences（p<0.01）.
Behaviors survey result shows that leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. Extract can be remarkably reinforced suspension index, pole-climbing index and the trip of mice
Swimming index, with the effect for significantly improving MPTP MODEL C 57BL/6 mice behaviors.
4. the serum pharmacological research of Broussonetia papyrifera heaf total phenolic acid anti-parkinson.
4.1. experiment material.
, using SD rats, male and female half and half, 200 ± 20g of body weight, purchased from Dalian Medical Univ for method.Leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. used is total
Phenolic acid is to be made by oneself using above-mentioned technique.Weigh leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract appropriate, be configured to the aqueous solution of 5 mg/mL, stand-by.
4.2. experimental technique.
4.2.1 the preparation of drug serum.
Rat adaptability is raised 3 days, and free water is taken food.It is randomly divided into blank group and Broussonetia papyrifera heaf total phenolic acid high and low dose
Group.High and low dose group is administered 150mg/kg, 75mg/kg respectively, daily gastric infusion 2 times, successive administration 7 days；Blank group gives
Normal saline, continuous to 7 days.Anaesthetize after 0.5 h after last dose, pluck eyeball and take blood, after standing 0.5 h, 3000
Rpm/min is centrifuged 10 min, separates serum, and then 56 DEG C of inactivation 30min to be to remove bioactive substance that may be present, and 0.22
μm filtering with microporous membrane is degerming, is placed in -20 DEG C of Refrigerator stores standby after sealing.
4.2.2 cell packet and process.
People's mother's neurocytoma system cell（SH-SY5Y cells, are purchased from Chinese Academy of Sciences's Shanghai cell bank）.With containing 10% inactivation tire
Ox blood serum, penicillin 100U mL-1, the DMEM F-12 culture fluid culture SH-SY5Y cells of 100 μ g mL-1 of streptomycin,
Every other day change culture fluid, cultivate to cell monolayer about 80% converge when, Secondary Culture is tested with the cell of exponential phase.
Adjustment cell density is 1 × 105It is inoculated in 96 orifice plates, per 100 μ L of hole, is placed in 37 DEG C, in the incubator of 5%CO2, culture
16 h treat cell attachment.Cell is randomly divided into 4 groups：Blank serum group, model group, the high low dose group of experiment, set 3 again per group
Hole.Experimental group is separately added into the complete medium of 2.1 lower high and low dose groups containing volume fraction 5%, 10% and 20%, blank blood
Clear group blank group blood serum medium only plus under 2.1 of respective volume fraction；Model group gives 2.1 lower moulds of response volume
The culture medium of type group serum.After 24 h are cultivated in 37 DEG C, the incubator of 5%CO2, experimental group and model group are simultaneously introduced 1mM
MPP+（It is purchased from sigma companies, lot number：72111）, continue 48 h of culture.Model group and Broussonetia papyrifera heaf total phenolic acid high and low dose
Group, cell per well add 5 mg/mL MTT, 10 μ L, cultivate 4 h, suck culture medium in hole, add 100 μ L of DMSO per hole, wait to tie
Brilliant fully dissolving, microplate reader detect the absorption value in each hole at 492 nm（OD values）（620 nm of reference wavelength）, calculate cell survival
Rate.
Cell survival rate %=experimental grouies OD values/blank group OD value × 100%.
Statistical procedures data are with mean ± standard deviation（±s）Represent, use one factor analysis of variance（one-way
ANOVA）Carry out statistical procedures.
4.3. experimental result.
Compared with blank serum group, model group cell survival rate is respectively（42.24% ± 1.71%）、（42.53% ±
1.09%）With（37.01% ± 0.97%）；The survival rate for being previously added the Broussonetia papyrifera heaf total phenolic acid Contained Serum group of high and low dose shows
Writing increases, and is statistically significant with model group, is shown in Table 4.As a result show, the Broussonetia papyrifera heaf total phenolic acid group of certain volume fraction contains
Medicine serum has significant protective effect to the SH-SY5Y cells that MPP+ is induced.
4 different volumes fraction of table Contained Serum antagonism MPP+ cytotoxicities after SH-SY5Y cell survival rates (±
s).
Note：* compare with model group, variant（p<0.05）；* is compared with model group, and there were significant differences（p<0.01）, * * *
Compare with model group, have pole significant difference（p<0.001）.
The specific embodiment of the present invention is above are only, but the design concept of the present invention is not limited thereto, all using this structure
Think the change that unsubstantiality is carried out to the present invention, all should belong to the behavior for invading the scope of the present invention.
Claims (4)
1. leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract is used for preparing antiparkinsonism drug.
2. Broussonetia papyrifera heaf total phenolic acid as claimed in claim 1, it is characterised in that described total phenolic acid extract total phenols acid content is big
In 50%, in heaf total phenolic acid extract cosmosiin content be 3-7,3,5,4 '-trihydroxy-Diphenethyl -3-O- β-D- Portugals
Polyglycoside is 1-3.
3. leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract as claimed in claim 1, it is characterised in that described total phenolic acid extract total phenolics
Content is preferably 55.1%.
4. the leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract as described in claim 1-3, it is characterised in that take the Broussonetia papyrifera of appropriate natural drying
Leaf, is ground into powder；Leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. powder is put into supercritical CO2In extraction kettle, first with 85% ethanol as entrainer, during extraction
Between be 1.5 h, extracting pressure is 50.0MPa, and extraction temperature is 50 DEG C, O2Flow is 10 kg h-1, and separating pressure is
5.8 MPa, separation temperature are 40 DEG C；The ethanol of gained after extraction, through concentrating under reduced pressure, drying under reduced pressure, obtains Broussonetia papyrifera heaf total phenolic acid
Extract.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107998685A (en) * | 2017-11-26 | 2018-05-08 | 长沙无道工业设计有限公司 | Chinese herbal medicine extracting process |
| CN115252676A (en) * | 2022-08-24 | 2022-11-01 | 刘连英 | Medicine for treating complications caused by prostatic hyperplasia and preparation method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN101450127A (en) * | 2009-01-12 | 2009-06-10 | 中国人民解放军第二军医大学 | Pawpaw total phenolic acid extract and use thereof |
| CN102319291A (en) * | 2011-08-19 | 2012-01-18 | 大连中植环境生物科技有限公司 | A kind of leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract and the application in the preparation cancer therapy drug thereof |
| CN102631388A (en) * | 2012-04-02 | 2012-08-15 | 辽宁中医药大学 | Protopanaxatriol composition and application thereof to preparation of Parkinson resisting drugs |
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|---|---|---|---|---|
| CN101450127A (en) * | 2009-01-12 | 2009-06-10 | 中国人民解放军第二军医大学 | Pawpaw total phenolic acid extract and use thereof |
| CN102319291A (en) * | 2011-08-19 | 2012-01-18 | 大连中植环境生物科技有限公司 | A kind of leaf of Broussonetia papyrifera (L.) L.Her.ex Vent. total phenolic acid extract and the application in the preparation cancer therapy drug thereof |
| CN102631388A (en) * | 2012-04-02 | 2012-08-15 | 辽宁中医药大学 | Protopanaxatriol composition and application thereof to preparation of Parkinson resisting drugs |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN107998685A (en) * | 2017-11-26 | 2018-05-08 | 长沙无道工业设计有限公司 | Chinese herbal medicine extracting process |
| CN115252676A (en) * | 2022-08-24 | 2022-11-01 | 刘连英 | Medicine for treating complications caused by prostatic hyperplasia and preparation method |
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