CN101371886A - Application of volatile oil of plant belonging to citrus in preparing medicament for treating diabetes - Google Patents
Application of volatile oil of plant belonging to citrus in preparing medicament for treating diabetes Download PDFInfo
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Abstract
The invention relates to the application of citrus plant volatile oil in the preparation of drugs for treating diabetes. The invention relates to new use of the volatile oil of fresh mature fruit skin of Citrus reticulata Blanco and the cultivated varieties, or the fresh mature fruit, fruit skin and seeds of C.aurantium, or the fresh mature fruit skin of Citrus aurantium and the cultivated varieties, or the fresh mature fruit and fruit skin of C.hystrix, or the fresh mature fruit and fruit skin of C.bergamia, as well as traditional Chinese medicine tangerine peel, green tangerine peel, immature bitter orange, bitter orange and the volatile oil in the preparation of the drugs for treating diabetes.
Description
Technical field the present invention relates to the Chinese medicine Pericarpium Citri Reticulatae, Pericarpium Citri Reticulatae Viride, Fructus Aurantii Immaturus, Fructus Aurantii and volatile oil thereof, and the fresh mature peel of rutaceae Fructus Citri tangerinae Citrus reticulata Blanco and variety thereof, or the fresh mature fruit of Fructus Citri sinensis C.aurantium, peel and seed, the perhaps fresh mature peel of Citrus aurantium Linn. Citrus aurantium, perhaps fresh mature fruit and the peel of Mauritius papeda C.hystrix, the perhaps purposes of volatile oil aspect medical science of the fresh mature fruit of oily orange C.bergamia and peel, relate in particular to new purposes, be specifically related to the new purposes aspect the medicine of preparation treatment diabetes at pharmaceutical field.
Above-mentioned Chinese medicine of background technology or plant volatile oil derive from the fresh mature peel or the dry mature skin (Chinese medicine Pericarpium Citri Reticulatae pericarpium Citri Reticulatae) of rue family citrus plant Fructus Citri tangerinae Citrusreticulata Blanco and variety thereof, or the peel of dry young fruit or immature fruit (Chinese medicine Pericarpium Citri Reticulatae Viride pericarpium Citri Reticulatae Viride), or the fresh mature fruit of Fructus Citri sinensis C.aurantium, peel and seed (seed), the perhaps fresh mature peel of Citrus aurantium Linn. Citrus aurantium, the perhaps peel of the dry young fruit of Citrus aurantium Linn. Citrusaurantium and variety thereof or Fructus Citri sinensis C.aurantium (Chinese medicine Fructus Aurantii Immaturus Fructus AurantiiImmaturus), perhaps fresh mature fruit and the peel of Mauritius papeda C.hystrix, perhaps fresh mature fruit and the peel of oily orange C.bergamia, the perhaps immature fruit of Citrus aurantium Linn. Citrus aurantium L. (Chinese medicine Fructus Aurantii Fructus Aurantii) and variety thereof.
Diabetes are diseases of a kind of complexity, it is characterized by hyperglycemia.This disease can be divided into two main subclass: insulin-dependent (IDDM), be also referred to as type 1 diabetes, and non-insulin-depending type (NIDDM), be also referred to as type 2 diabetes mellitus.
1 type diabetes form when the immune system of body has been destroyed beta Cell of islet, and this cell is the cell of the hormone insulin of manufacturing blood sugar regulation unique in body.Type 1 diabetes may account for the 5-10% of the diabetes of all having diagnosed
2 type diabetes may account for the 90-95% of the diabetes of all having diagnosed.It has insulin resistant when beginning usually, a kind of disorder that can not fully utilize insulin with cell.When the needs insulin increased secretion, pancreas was lost the ability that it produces insulin gradually.
1 type and type 2 diabetes mellitus can both cause complication blood capillary, trunk and neuropathy.Diabetes can influence the mass part of body, and can cause serial complication for example to resemble blind, kidney injury and amputation.
2003, Chinese disease prevention and control centre estimated, nearly 4,000 ten thousand diabetes patients of China.2005, Center for Disease Control of national sanitary portion estimated that the U.S. has 2,080 ten thousand people that diabetes are arranged, and accounts for 7.0% of population.2002, in the U.S., the total cost of diabetes was 1,320 hundred million dollars (comprising flat cost and overhead cost), and directly medical expense is 92,000,000,000 dollars, and indirect expense is 40,000,000,000 dollars (comprising permanent disability, disablement and premature dead).European Union estimated in 2005, and Europe has about 2,250 ten thousand people to suffer from type 2 diabetes mellitus.2005, World Health Organization (WHO) estimated that global diabetics is 1.8 hundred million.
At present, the medicine of the type 2 diabetes mellitus of treatment mainly contains following a few class:
1. biguanides.Curative effect is low, and side effect is bigger, for a long time with the normal lactic acidosis that produces.
2. sulphur is urinated the class medicine.Side effect is bigger, for a long time with the normal hypoglycemia that produces.And it is tired that the pancreas island is taken place, even depleted, and endogenic insulin secretion function further reduces, and the medicine secondary failure takes place.
3. acarbose class medicine.Only postpone the absorption of glucose, reduce level of postprandial blood sugar at small intestinal.It can not stimulate secretion of insulin, can not change fasting insulin and C peptide level.
4. above-mentioned these medicines cooperate insulin or make the insulinize type 2 diabetes mellitus separately, and final endogenic insulin secretion function will completely lose, and the dependency of insulin will be come eventually.
Above-mentioned all medicines all have the effect of more intense and persistent blood sugar lowering, but blood sugar lowering level stably.Simultaneously, patient will need to strengthen year by year the therapeutic dose of these medicines.Britain's diabetes perspective study points out that all medicines all can not prevent fasting plasma glucose level persistence to rise, and show that islet cell function carries out sexual exhaustion.
Prior art and knowledge only disclose Chinese medicine Radix Ginseng Radix Ginseng (root of Araliaceae Radix Ginseng Panax ginseng C.A.Mey.), Rhizoma Polygonati Odorati Rhizoma Polygonati Odorati (liliaceous plant Rhizoma Polygonati Odorati Polygonatum odoratum (Mill.) Druce rhizome), Radix Glycyrrhizae Radix Glycyrrhizae (root and rhizome of glycyrrhizic legume Glycyrrhiza uralensis Fisch. or Glycyrrhiza inflata Bat. G.inflate Bat. or Glycyrrhiza glabra L. G..glabra L.), Cortex Lycii Cortex Lycii (root bark of matrimony vine of solanaceae plant Lycium chinense Mill. or lycium barbarum Lycium barbarum L.), Rhizoma Atractylodis Rhizoma Atractylodis (feverfew Atractylodes lancea (Thunb.) DC. Atracylodes lancea (Thunb.) D.C. or Atractylis chinensis A.chinensis (D.C.) Koidz. rhizome), Rhizoma Atractylodis Macrocephalae Rhizoma Atractylodis Macrocephalae (feverfew Rhizoma Atractylodis Macrocephalae Atractylodesmacrocephala Koidz. rhizome), Rhizoma Anemarrhenae Rhizoma Anemarrhenae (liliaceous plant Rhizoma Anemarrhenae Anemarrhena asphodeloides Bunge rhizome), Semen Litchi Semen Litchi (seed of sapindaceous plant Fructus Litchi Litchi chinensis Sonn.), Folium Mori Folium Mori (leaf of moraceae plants Mulberry Morus alba L.), Rhizoma Coptidis Rhizoma Coptidis (cohosh Rhizoma Coptidis Coptis chinensis Franch. or Coptis deltoidea C.Y.Cheng et Hsiao C.deltoidea C.Y.Cheng et Hsiao or Coptis Teeta Wall C.teeta wall. rhizome), Rhizoma Polygonati RhizomaPolygonati (liliaceous plant Yunnan Rhizoma Polygonati Polygonatum kingianum Coll.et Hemsl. or Rhizoma Polygonati P.sibiricum Red. or Polygonatum cyrtonema Hua P.cyrtonema Hua rhizome), Herba Epimedii Herba Epimedii (aerial parts of Berberidaceae plant Herba Epimedii Epimedium brevicornum Maxim. or arrow leaf Herba Epimedii E.sagittatum (Sieb.Et Zucc.) Maxim. or Herba Epimedii E.Koreanum Nakai), Radix Puerariae RadixPuerariae (root of legume pueraria lobata Pueraria lobata (Willd.) Ohwi or Radix Puerariae P.thomsonii Benth.) and Radix Arnebiae (Radix Lithospermi) Radix Amebiae (root of comfrey lithospermum euchromum Royle Arnebia euchroma (Royle) JAohnst. or Radix Arnebiae (Radix Lithospermi) Lithospermum erythrorhizon Sieb.et Zucc.) and extract thereof etc. have the effect of blood sugar lowering.
Summary of the invention the object of the present invention is to provide from the fresh mature fruit of orange and the volatile oil in the peel, and their dry fruit and peel comprise the new medical use of Chinese medicine Pericarpium Citri Reticulatae, Pericarpium Citri Reticulatae Viride, Fructus Aurantii Immaturus and Fructus Aurantii and volatile oil thereof.Be that they are at pharmaceutical field, in the application aspect the medicine of preparation treatment diabetes.The present invention finds by zoopery and clinical observation, above-mentioned plant, and perhaps plant medical herbs or Chinese medicine all have the effect of obviously and strong blood sugar lowering separately.
The present invention finds in clinical observation that at first Chinese medicine Pericarpium Citri Reticulatae etc. has the effect of tangible reduction type 2 diabetes mellitus people post-prandial glycemia.
Further study the volatile oil Oleum Citri Reticulatae of observing the Chinese medicine cutification more powerful blood sugar lowering effect is arranged.By zoopery, find they have simultaneously reduce inductive 1 type of medicine STZ-and and the obvious effect of type 2 diabetes mellitus rat model blood glucose, the therapeutic effect highly significant.
Existing knowledge and technology shows, Citrus, and orange class plant comprises Fructus Citri tangerinae, Fructus Citri sinensis, Citrus aurantium Linn., Mauritius papeda, oily orange belongs to similar fruit, the biochemical property basically identical of their fresh mature fruit and volatile oil in the peel and Oleum Citri Reticulatae.
From fruit, peel and the leaf of rue family citrus plant, extract the method for volatile oil:
Adopt rutaceae Fructus Citri tangerinae Citrus reticulata Blanco and the dry mature skin Pericarpium Citri Reticulatae pericarpium Citri Reticulatae of variety or the peel Pericarpium Citri Reticulatae Viride pericarpiumCitri Reticulatae Viride of dry young fruit or immature fruit, perhaps adopt the peel of the dry young fruit of Citrus aurantium Linn. Citrus aurantium and variety thereof or Fructus Citri sinensis C.aurantium (Chinese medicine Fructus Aurantii Immaturus Fructus Aurantii Immaturus), perhaps adopt the fresh mature peel of rutaceae Fructus Citri tangerinae Citrus reticulata Blanco and variety thereof, or the fresh mature fruit of employing Fructus Citri sinensis C.aurantium, peel and seed (seed), perhaps adopt the fresh mature peel of Citrus aurantium Linn. Citrusaurantium, perhaps fresh mature fruit and the peel of Mauritius papeda C.hystrix, perhaps adopt fresh mature fruit and the peel of oily orange C.bergamia, tear up directly cold pressing of back, through centrifugal or filtration, obtain volatile oil again.
Use the dry fruit skin, it is soft to add 8 parts of blisters with every part of peel, generally steeps 3-4h.
Perhaps, directly adopt fruit to get oil.Select the Citrus of cleaning for use, orange class fruit is sent in the different oil grinding machines and is got oil.Mainly contain FMC technology and Blang's technology (BOE), also have special oil grinding machine in some areas and country.
Perhaps, behind other conventional method separation and purifications, can be with in the present invention.Also can adopt commercially available product, the granule of the Pericarpium Citri Reticulatae of producing as Jiangsu Tian Jiang drugmaker, Pericarpium Citri Reticulatae Viride, Fructus Aurantii Immaturus, Fructus Aurantii.All kept the volatile oil that contains in this kind Chinese medicine crude drug in these granules.
Extracting the method for volatile oil from Chinese medicines such as Pericarpium Citri Reticulatae, Fructus Aurantii Immaturus, Fructus Aurantii, mainly is the resulting and immiscible volatility oily of water composition with steam distillation.
In zoopery of the present invention, this product shows the obvious functions of blood sugar effect to drug-induced diabetes rat.Oral administration gives in laboratory animal Pericarpium Citri Reticulatae and extractive of volatile oil (7-8g/kg/ days) thereof back 0.5-2 hour, and the maximum reduction amplitude of animal blood slurry glucose can reach 50%～100%, and drug effect fades away after 2 hours in medication.
The present invention can reduce medicine (streptozotocin, streptozotocin) fasting glucose and the diabetic complication symptom of the severe type 2 diabetes mellitus rat model due to inducing.Give diabetes rat with Pericarpium Citri Reticulatae and the extractive of volatile oil filling stomach thereof of 7-8g/kg, after 0.5-2 hour, the blood glucose of experimental rat obviously descends.
In clinical experiment, the type 2 diabetes mellitus patient took Pericarpium Citri Reticulatae and extractive of volatile oil thereof, 12 weeks by a definite date after the meal in every day 3.The result shows that this medicine can obviously reduce medication person's blood sugar level after the meal.The speed of blood sugar lowering is quick, stable.
In clinical experiment, the type 2 diabetes mellitus patient took respectively Pericarpium Citri Reticulatae Viride, Fructus Aurantii Immaturus, Fructus Aurantii and extractive of volatile oil thereof, 12 weeks by a definite date after the meal in every day 3.The result shows that these Chinese medicines can obviously reduce medication person's blood sugar level after the meal.The speed of blood sugar lowering is quick, stable, and still, the effect of blood sugar lowering is worse than Pericarpium Citri Reticulatae slightly.
Healthy people takes Oleum Citri Reticulatae and does not then reduce normal blood sugar level.
Zoopery and clinical experiment result show, the new medical usage of above-mentioned a series of plants, plant medical herbs or the Chinese medicine that the present invention relates to, find that said medicine has the effect of stronger blood sugar lowering, and these plants, plant medical herbs or Chinese medicine the effect of blood sugar lowering is arranged is never to report.
Zoopery shows that Oleum Citri Reticulatae can not reduce the normal blood sugar level of healthy rat.
The present invention can contain one or more pharmaceutically acceptable carriers, it is the pharmaceutical carrier of pharmaceutical field routine, as water, starch, cellulose, gelatin, agar, powder-beta-dextrin, fabaceous lecithin or lecithin etc., medicine of the present invention can be by mode administration oral and injection.During oral administration, preferred form is tablet, coated tablet, granule, powder, soft capsule and suppository.Common dose of the present invention can be according to the order of severity of route of administration, conditions of patients, variations such as the type of diabetes, daily dose can be Pericarpium Citri Reticulatae concentrated granule 3-6g (being equivalent to crude drug 18-36g), preferred every day dosage 4-5g.Have meal every day three times, divide and take for three times.The number of times of perhaps taking medicine is identical with the dining number of times.Take in 1-10 minute behind first mouthful of food inlet.
When using Oleum Citri Reticulatae separately, slight xerostomia phenomenon is arranged after part patient's medication; Giddy, weak, cardiopalmus, constipation or diarrhoea, herpes labialis etc. can appear in small number of patients, can disappear in decrement or drug withdrawal afterreaction.But use the Chinese medicine Pericarpium Citri Reticulatae contain capacity volatile oil, perhaps Rhizoma Et Radix Notopterygii etc. does not all have any significant side effects.
Healthy people takes above-mentioned any medicine separately, does not reduce after the meal or fasting blood glucose level.
The several embodiment of specific embodiment various details, but content of the present invention is not limited to this fully.
Detailed description of the invention
Embodiment 1
From above-mentioned fresh peel or dry peel, extract volatile oil:
Adopt the fresh mature peel of Fructus Citri tangerinae or orange, directly send cold pressing in the squeezer after tearing up to, perhaps adopt the dry mature skin Pericarpium Citri Reticulatae pericarpiumCitri Reticulatae of rutaceae Fructus Citri tangerinae Citrus reticulata Blanco and variety thereof, it is soft to add 8 parts of blisters with every part of peel, generally steeps 3-4h.
1. soak
Peel is immersed in 1.5～3.0% the lime water, keeps pH value 10～14, soak 6-10h, peel and lime water ratio are between 1:4～6.The sclerosis peel makes it to be fit to squeezing.Outside the delime, soak and also can use sodium bisulfate, sodium carbonate, sodium sulfate etc.
2. clean
Peel is put into clear water flush away Calx and impurity.
3. squeezing
By squeezer the quintessence oil in the peel is extruded.
4. filter or separation
Oil water mixture from cold pressing expeller or squeezer come out has more peel residue, has indefinite stickiness, need filter and precipitation repeatedly, to alleviate the pressure of centrifugal separator.Filtering residue can reclaim quintessence oil through distillator.
5. centrifugalize
The high speed oil-water separator is adopted in the separation of profit, carries out oil-water separation.Separate finish after, should allow seperator idle running 2-3min, pour clear water in a large number, remaining oil is gone out.In order to impel quintessence oil from fat liquor, to separate, can add about 2% sodium hydroxide and a small amount of sodium sulfate in the water of oil expression usefulness.
6. make with extra care and dewaxing
Slight muddy thick quintessence oil is used anhydrous sodium sulfate dehydration, leaves standstill elimination residue, centrifugalize again behind the clear then.Muddy thick quintessence oil, through staticly settling, the reuse anhydrous sodium sulfate filters earlier.Precipitation residue, centrifugalize again.
Thick quintessence oil placed under the low temperature leave standstill, after waxiness was separated out, the reuse centrifugal separation was removed.Under 8 ℃ of conditions, can separate out about 6d.Under-25 ℃, 2d can separate out.
7. volatile oil is made the tablet that to take
Volatile oil and a certain amount of β-dextrin is mixed in the mode of spraying, add a certain amount of water, keep at a certain temperature, stir 1h, 24h is placed in cold preservation, filters, drains, adding a small amount of ether washing 2 times, is powder in drying below 50 ℃, adds the water of capacity again.The rate of charge of volatile oil and β-CD is 1:9 (ml:g), mixing speed 100r/min, and comprising temperature is 40 ℃, β-dextrin is 1:7 with the ratio that adds entry.
Embodiment 2
2 type diabetes model rat experiments
The foundation of 2 type diabetes rat experimental models.Adopt the model of the similar NIDDM of anaphylactic type.One time capacity gives animal Wistar rat streptozotocin (STZ), causes the B cell to damage in a large number, and the synthetic and secretion of insulin reduces, and causes carbohydrate metabolism disturbance, causes diabetes.STZ with fresh 2% the solution of being mixed with of the minor official sour sodium buffer of the aseptic Chinese holly of 0.1mol/L, is regulated pH value to 4.5, the bacteria filter filtration sterilization.50-65mg/kg tail vein shot solution was pressed in the rat fasting in 18 hours, and 24 hours blood glucose promptly can be used as successfully model more than or equal to 13.0mmol/L (perhaps 16.7mml/L).
The common single dose of this anaphylactic type model reaches the 50mg/kg body weight and the natural remission phenomenon do not occur, and 6-27 days, islets of langerhans had to a certain degree regeneration, and funtion part is recovered, but does not reach normal, still is in hyperglycemia state, is suitable for the correlational study of NIDDM.Streptozotocin is injected in back 10 hours and is given rat feeding 10% glucose, to prevent hypoglycemia.
In relevant experiment, STZ is dissolved into 0.1M citric acid-sodium citrate buffer (PH4.2) to form 60-65mg/kg weight solution, and solution is injected into the tail vein immediately.After 10 days, after determining the abundant height of check blood sugar level, experiment material is given.Treated 7 days, and used canonical procedure to obtain blood from animal, the SureStep blood glucose machine of a blood glucose monitoring system Johson ﹠ Johnson is used in measurement of glucose levels.
In relevant method for breeding, use 7 all big male Wistar rats (weight 210 ,+-.10g) 12 every group.Mouse is raised humidity 50% and illumination-dark each 12 hours (the about 19:00 of 7:00) under room temperature 22 degree Celsius.Ad lib and drinking-water.Every day is in fasting after 4 hours, adds Pericarpium Citri Reticulatae and extractive of volatile oil enters in the mouse body with 5g/kg (being equivalent to crude drug 30g/kg) body wt in the mode of irritating stomach.Irritated behind the stomach 2 hours, and, checked its blood sugar level from the rat tail vein blood drawing.
Experimental result as shown in Table 2, t〉0.001, there are the difference of highly significant, curative effect highly significant in P＜0.01.Illustrate that medicine Pericarpium Citri Reticulatae and volatile oil thereof can obviously reduce the fasting glucose of type 2 diabetes mellitus rat model.
Compare before and after the treatment of table 1:2 type diabetes model rat (x ± S)
| Number of animals | Fasting glucose | Medicine (g/kg) | |
| Before the treatment | 10 | 25.94±2.281 | |
| After the treatment | 10 | 19.15±4.952 | Pericarpium Citri Reticulatae granule dosage 5g |
| t | 3.938 | ||
| p | <0.01 |
Embodiment 3:
The experiment of 1 type diabetes rat
The foundation of 1 type diabetes rat experimental model.Recent years, more people have clearly expressed and have observed the inductive experiment diabetes model of STZ-, though it is 1 pattern type in principle, may be used for as same 2 pattern types.In any case, because the inductive experiment diabetes model of STZ-mouse 1-6 days after morbidity, the similar type 1 diabetes that depends on insulin, further research is to go to detect blood sugar level carefully.
The inductive diabetes rat of STZ-, in injection 3-6 days behind the STZ, every day is in fasting after 4 hours, adds Pericarpium Citri Reticulatae and extractive of volatile oil enters in the mouse body with 7.2g/kg (being equivalent to crude drug 43g/kg) body wt in the mode of irritating stomach.Irritated behind the stomach 2 hours, and, checked its blood sugar level from the rat tail vein blood drawing.
Experimental result shows that Oleum Citri Reticulatae has obvious therapeutic effect to the type 1 diabetes rat model.(table 2 of face as follows)
The comparison of table 2 IDDM group and Pericarpium Citri Reticulatae and extractive of volatile oil treatment group blood sugar level thereof (x ± S)
Embodiment 4
One male patient, 56 years old, type 2 diabetes mellitus.Fasting glucose is in normal range, and post-prandial glycemia is in 15.6mmol/L (281mg/dl) scope.After 4-6 hour after the meal, do not have feed, hypoglycemia often takes place.Treat in preceding 15 days and do not use any medicine, by 7 days treatment, every day breakfast, lunch and dinner, at first mouthful in 5-10 minute after meal, orally give Oleum Citri Reticulatae 1.3ml, blood glucose peak-peak 11.8mmol/L (212mg/dl) is at 1.25h after breakfast.Continue 12 weeks of treatment, blood glucose peak-peak 10.4mmol/L (212mg/dl) is advanced to 0.75h after breakfast.After the dinner, refuse to obey any medicine, post-prandial glycemia is in normal range.After the lunch, do not take medicine post-prandial glycemia or in normal range, perhaps blood sugar level is high slightly.But breakfast still can not drug withdrawal.After 6-8 hour after the meal, there is not feed, no hypoglycemic reaction.Patient keeps the medium diet of appropriateness during this period.
This patient tested preceding 3 months at this, and every meal employing Pericarpium Citri Reticulatae 2g (crude drug 12g) treats and reaches 3 months, reduces post-prandial glycemia more stablely.Lunch and dinner post-prandial glycemia in normal range, only after breakfast blood glucose still more than 11.1mmol/L (200mg/dl).
Embodiment 5
One women, 58 years old,
Historical 15 years of diabetes, fasting glucose is between 8.6-13mmol/L (155-234mg/dl), and post-prandial glycemia is up to 23mmol/L (414mg/dl).By 7 days treatment, every day breakfast, lunch and dinner, at first mouthful in 5-10 minute after meal, orally give Oleum Citri Reticulatae 1.3ml, blood glucose peak-peak 18.7mmol/L (337mg/dl) is at 1.25h after breakfast.Contrast before and after the treatment, blood glucose on average reduces the medium diet that 6.99mmol/L (126mg/dl) patient keeps appropriateness during this period.
Embodiment 6
Healthy volunteer's experiment
One male, 24 years old, healthy no any disease.Fasting glucose is in 4.4-5.3mmol/L (79-96mg/dl) fluctuation, and post-prandial glycemia is between 6-7mmol/L (108-126mg/dl).By 7 days treatment, every day breakfast, lunch and dinner, at first mouthful in 5-10 minute after meal, orally give Oleum Citri Reticulatae 1.2ml, blood glucose peak-peak 5.6-8.0mmol/L (101-144mg/dl) is at 0.5h after breakfast.Contrast before and after the treatment, the basic no change of blood glucose.The experimenter keeps the medium diet of appropriateness during this period.
Embodiment 7
Healthy volunteer's experiment
One women, 46 years old, healthy no any disease.Fasting glucose is in 5.0-5.4mmol/L (79-96mg/dl) fluctuation, and post-prandial glycemia is between 8-12.2mmol/L (144-202mg/dl).The carbohydrate tolerance experiment is unusual.By 7 days treatment, every day breakfast, lunch and dinner, at first mouthful in 5-10 minute after meal, orally give Oleum Citri Reticulatae 1ml, blood glucose peak-peak 10.3-11.7mmol/L (185-211mg/dl) is at 0.5h after breakfast.Contrast before and after the treatment, the basic no change of blood glucose.The experimenter keeps the medium diet of appropriateness during this period.
Industrial applicability
Raw material is mainly from fresh maturate peel or the dry mature skin (Chinese medicine dried orange peel pericarpium Citri Reticulatae) of rutaceae tangerine Citrus reticulata Blanco and variety thereof, or the pericarp of dry young fruit or immature fruit (Chinese medicine rascal pericarpium Citri Reticulatae Viride), or fresh ripening fruits, pericarp and the seed (seed) of sweet orange C.aurantium, the perhaps fresh maturate peel of bitter orange Citrus aurantium, the perhaps pericarp of the dry young fruit of bitter orange Citrus aurantium and variety thereof or sweet orange C.aurantium (traditional Chinese medicine citrus aurantium Fructus Aurantii Immaturus), perhaps fresh ripening fruits and the pericarp of Mauritius papeda C.hystrix, perhaps fresh ripening fruits and the pericarp of oily orange C.bergamia. Raw material sources are abundant and extensive, and output is high.
Secondly, processing method is simple, and the simplest processing method is to obtain Oleum Citri Reticulatae by the mode of squeezing.
Above-mentioned essential oil can carry out oil mixing with water by high-shear emulsion machine easily, makes injection; Perhaps add in the powder-beta-dextrin with spray pattern and make tablet, also can directly make the finish soft capsule. Therefore, be suitable for suitability for industrialized production.
Claims (9)
1. Chinese medicine Pericarpium Citri Reticulatae and volatile oil thereof the application in preparation treatment diabetes medicament.
2. Chinese medicine Pericarpium Citri Reticulatae Viride and volatile oil thereof the application in preparation treatment diabetes medicament.
3. Chinese medicine Fructus Aurantii Immaturus and volatile oil thereof the application in preparation treatment diabetes medicament.
4. Chinese medicine Fructus Aurantii and volatile oil thereof the application in preparation treatment diabetes medicament.
5. the application of the volatile oil of Fructus Citri tangerinae in preparation treatment diabetes medicament.
6. the application of the volatile oil of Fructus Citri sinensis in preparation treatment diabetes medicament.
7. the application of the volatile oil of Citrus aurantium Linn. in preparation treatment diabetes medicament.
8. the application of the volatile oil of Mauritius papeda C.hystrix in preparation treatment diabetes medicament.
9. the application of the volatile oil of oily orange C.bergamia in preparation treatment diabetes medicament.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2009053793A2 (en) * | 2007-08-27 | 2009-04-30 | Gang Shu | Use of volatile oil from plants in preparing medicaments for preventing and treating diabetes |
| CN104940363A (en) * | 2015-06-20 | 2015-09-30 | 浙江大学 | Vitro and vivo hypoglycemic application of trifoliate orange extractive |
| CN108651663A (en) * | 2018-08-13 | 2018-10-16 | 佛山市三水区嘉华化学研究院（普通合伙） | One kind is quenched one's thirst warm kidney health protection tea and preparation method thereof |
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2007
- 2007-08-24 CN CNA2007100125632A patent/CN101371886A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2009053793A2 (en) * | 2007-08-27 | 2009-04-30 | Gang Shu | Use of volatile oil from plants in preparing medicaments for preventing and treating diabetes |
| WO2009053793A3 (en) * | 2007-08-27 | 2009-07-09 | Gang Shu | Use of volatile oil from plants in preparing medicaments for preventing and treating diabetes |
| CN104940363A (en) * | 2015-06-20 | 2015-09-30 | 浙江大学 | Vitro and vivo hypoglycemic application of trifoliate orange extractive |
| CN104940363B (en) * | 2015-06-20 | 2018-04-17 | 浙江大学 | A kind of inside and outside hypoglycemic purposes of trifoliate orange extract |
| CN108651663A (en) * | 2018-08-13 | 2018-10-16 | 佛山市三水区嘉华化学研究院（普通合伙） | One kind is quenched one's thirst warm kidney health protection tea and preparation method thereof |
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