CN101366357A - Method for tissue culture and quick propagate technique of reddish blue spider lily - Google Patents
Method for tissue culture and quick propagate technique of reddish blue spider lily Download PDFInfo
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Abstract
The invention discloses a method for the tissue culture of Lycoris haywardii and a rapid propagation technique. The method comprises the following: 1) a step of preparing culture mediums, and components of a basic culture medium and the culture mediums in every stage of tissue culture, as well as the weight of every component contained in each liter are as follows: the basic culture medium comprises MS or 1/2MS, wherein the basic culture medium comprises 20 to 40 g/L of sucrose and 8 g/L of agar and has the pH of 5.8; an induction culture medium comprises MS, 0.2 to 1.0 mg/L of TD and 2 g/L of activated carbon; a proliferation culture medium comprises MS, 3 to 7 mg/L of 6-BA and 0.5 to 2.5 mg/L of NAA; a strong seedling culture medium comprises MS, 0.5 to 2.5 mg/L of 6-BA and 0.5 to 1.5 mg/L of NAA; and a rooting culture medium comprises 1/2MS, 0.5 to 2.5 mg/L of KT, 0.5 to 2.5 mg/L IBA and 2 g/L of activated carbon; 2) a step of selecting and sterilizing explants; 3) a step of carrying out induction culture, proliferation culture, strong seedling culture and rooting culture; and 4) a step of domesticating and transplanting tissue culture seedlings. The method has the advantages that the induction rate of Lycoris haywardii buds reaches over 90 percent; the proliferation rate of each week is over 500 percent; the growth rate of the Lycoris haywardii buds is accelerated; the browning phenomenon of the explants is effectively prevented; and the rooting of the tissue culture seedlings is promoted at the same time when bulblet browning is prevented.
Description
Technical field
The present invention relates to field of plant tissue culture technique, mainly is a kind of tissue culture of red blue stone garlic and the method for quick propagating technology.
Background technology
Lycoris (Lycoris Herb) is universally acknowledged famous flowering bulb, is described as " Chinese tulip ".It is the herbaceos perennial of the underground bulb of a class tool, has abundant colored type and color.This genus whole world has 20 kinds.China's lycoris plants has 15 kinds, accounts for 3/4ths of whole world sum.
Red blue stone garlic (Lycoris haywardii) is a kind of natural crossing offspring in the Lycoris, and occurring in nature distributes seldom, and its flower is for pale purple rose-colored, and the petal top is a navy blue, and color is very beautiful.Flowering time is in 7～September, and the florescence happens autumn in summer high temperature and spends the time less, is a kind of rare high temperature season kind of admiring the beauty of flowers; Its leaf bottle green, vein tool canescence band, tall and straight upright, grow thickly, the likeness in form fragrant thoroughwort, attitude is quiet and tastefully laid out, growing up to from the leafing to the leaf is that the best is seen the leaf phase, still a kind of winter good reward leaf plant.
Short-tube lycoris is at the cultivation history in existing more than 1500 year of China, but never is widely used in afforestation and fresh cut-flowers and payes attention to.Main cause is that lycoris plants is bred based on natural bulb separation, and the bloom mother bulb average of ability of tool is only and is produced 1～2 bulbec, and reproduction coefficient is quite low; The particularly red blue stone garlic of short-tube lycoris seed is difficult for normally solid, and is also not high by the seminal propagation coefficient, and seedling 4～5 years of need from growing to blooming, and this has just greatly restricted the scale development and application of this good flower variety.In recent years, the tissue culture of short-tube lycoris is operated in domestic progressively expansion, but only is confined to short-tube lycoris, Lycoris aurea, in the common kind such as Bulbus Lycoridis longitubae, find no in gardens and the huge red blue stone garlic of fresh cut-flowers field development potentiality carry out the tissue culture of system and quick propagating technology research.
Summary of the invention
The present invention will solve the defective of above-mentioned prior art; a kind of tissue culture of red blue stone garlic and the method for quick propagating technology are provided; by having collected the germ plasm resource of a large amount of red blue stone garlics; pass through modern biotechnology; carry out vegetative propagation with method for plant tissue culture; for protecting red blue stone garlic germ plasm resource, satisfy in gardens simultaneously and the fresh cut-flowers field to its demand that increases day by day.
The present invention solves the technical scheme that its technical problem adopts.The tissue culture of this red blue stone garlic and the method for quick propagating technology, this method is carried out according to the following steps:
1), culture medium preparation, comprise that each component and every liter of contained weight of minimal medium and each stage medium of group training is:
(1) minimal medium: MS, sucrose 20～40g/L wherein, agar 8g/L, pH5.8;
(2) inducing culture: MS+TDZ0.2～1.0mg/L;
(3) proliferated culture medium: MS+6-BA3～7mg/L+NAA0.5～2.5mg/L;
(4) strong seedling culture base: MS+6-BA0.5～2.5mg/L+NAA0.5～1.0mg/L;
(5) root media: 1/2MS+KT0.5～2.5mg/L+IBA0.5～2.5mg/L+ active carbon 2g/L;
2), explant selection and sterilization treatment: select for use the red blue stone garlic bulb of robust growth to carry out sterilization treatment, the explant sampling is placed on for 4～May, and the short-tube lycoris scape was extracted out preceding 2 months; After the sterilization treatment bulb cut the height that stays apart from 0.8～1.3 centimetre of bulb basal disc, each bulb is on average to being cut to 4, after with the inside and outside two-layer incision of every branch, every has 3～5 layers of scale;
3), inducing culture: the explant after the sterilization treatment is placed on suck dry moisture on the filter paper of sterilizing, inserts on the inducing culture and carry out inducing culture, explant induction is cultivated after 3 days scale and is expanded and curl, and as seen grain of rice shape projection was arranged between scale after 12～15 days;
4), enrichment culture: inducing culture is after 15 days, will grow on the inducing culture explant and transfer carry out enrichment culture on enrichment culture; Cultivate and differentiate clump bud after 15～20 days; Every 15～20 days, the clump bud that breaks up is cut into individual plant inoculates on proliferated culture medium, again differentiation clump bud;
5), strong seedling culture: the clump bud that will differentiate is cut into individual plant and is inoculated on the strong seedling culture base, and the budlet base portion expands into clove after 15～20 days, and diameter is 0.3～0.8 centimetre;
6), culture of rootage: the clove after the strong seedling culture is inoculated on the root media, and the clove base portion bore 2～7 root systems in 10～15 days;
7), the domestication of tissue cultivating seedling and transplanting: when culture of rootage in the time of 20 days, the clove that will take root moves under the normal temperature and adapts to 3～5 days, open the cultivation bottle cap and adapt to 3～5 days again, be transplanted to behind the clean root medium spun yarn is housed: peat: perlitic volume ratio is in the cave dish of 2:1:1 matrix, waters permeable.
Described sterilization treatment is that red blue stone garlic bulb is cleaned, excision root and bulb neck, divest the outside 2 layers of scale of brown stain part and bulb, soaked after 10～20 minutes the flowing water flushing 2 hours with liquid detergent, the back is 75% alcohol disinfecting 1 minute with volume ratio, volume ratio is that 0.1% mercuric chloride solution soaked aseptic water washing 4～5 times 10～15 minutes.
Described medium comprises that each component and every liter of contained weight of minimal medium and each stage medium of group training is:
(1) minimal medium: MS or 1/2MS, wherein sucrose 20g or 40g/L, agar 8g/L, pH5.8;
(2) inducing culture: MS+TDZ0.4mg/L+ active carbon 2g/L, sucrose 40g;
(3) proliferated culture medium: MS+6-BA5mg/L+NAA1.0mg/L, sucrose 40g;
(4) strong seedling culture base: MS+6-BA2mg/L+NAA0.5mg/L, sucrose 40g;
(5) root media: 1/2MS+KT1.0mg/L+IBA2mg/L+ active carbon 2g/L, sucrose 20g.
The condition of culture in described each tissue culture stage is, culturing room's temperature is 24 ± 2 ℃, and light application time is that 12h/d, intensity of illumination are 25001x～3000Lx.
The invention has the beneficial effects as follows:
1) method of the red blue stone garlic tissue-culturing rapid propagation of Ti Chuing adopts inducing culture, proliferated culture medium, strong seedling culture base and root media that is fit to red blue stone garlic group training and the method for practicing seedling and transplanting, and the inductivity of red blue stone garlic budlet reaches more than 90%; The rate of increase in each cycle is more than 500%;
2) strong seedling culture through adding is accelerated red blue stone garlic budlet growth rate, and the budlet base portion expands into the clove that diameter is 0.3～0.8cm after 15～20 days, for next step culture of rootage is established solid foundation;
3) add active carbon in the inducing culture stage and effectively prevented the explant browning phenomenon, and add active carbon, when preventing the clove brown stain, also promoted taking root of tissue cultivating seedling, make rooting rate reach 100% in the culture of rootage stage;
4) at the consumption of inducing, propagation, three stages of strong sprout suitably increase sucrose, for the quick growing multiplication of red blue stone garlic group training budlet and clove provides safeguard.
Embodiment
The present invention is described in further detail by following examples, but content of the present invention is not limited thereto.
Embodiment 1: in the present embodiment 1, the technical method of the tissue culture of red blue stone garlic and breeding fast carries out according to the following steps:
1), culture medium preparation, comprise that each component and every liter of contained weight of minimal medium and each stage medium of group training is:
(1) minimal medium: MS, sucrose 20～40g/L wherein, agar 8g/L, pH5.8;
(2) inducing culture: MS+TDZ0.2～1.0mg/L+ active carbon 2g/L;
(3) proliferated culture medium: MS+6-BA3～7mg/L+NAA0.5～2.5mg/L;
(4) strong seedling culture base: MS+6-BA0.5～2.5mg/L+NAA0.5～1.0mg/L;
(5) root media: 1/2MS+KT0.5～2.5mg/L+IBA0.5～2.5mg/L+ active carbon 2g/L;
2), explant selection and sterilization treatment: select for use the red blue stone garlic bulb of robust growth to carry out sterilization treatment, the explant sampling is placed on for 4～May, and the short-tube lycoris scape was extracted out preceding 2 months; Sterilization treatment is that red blue stone garlic bulb is cleaned, excision root and bulb neck, divest the outside 2 layers of scale of brown stain part and bulb, soaked after 10～20 minutes the flowing water flushing 2 hours with liquid detergent, the back is 75% alcohol disinfecting 1 minute with volume ratio, volume ratio is that 0.1% mercuric chloride solution soaked aseptic water washing 4～5 times 10～15 minutes; After the sterilization treatment bulb cut the height that stays apart from 0.8～1.3 centimetre of bulb basal disc, each bulb is on average to being cut to 4, after with the inside and outside two-layer incision of every branch, every has 3～5 layers of scale;
3), inducing culture: the explant after the sterilization treatment is placed on suck dry moisture on the filter paper of sterilizing, inserts on the inducing culture and carry out inducing culture, explant induction is cultivated after 3 days scale and is expanded and curl, and as seen grain of rice shape projection was arranged between scale after 12～15 days;
4), enrichment culture: inducing culture is after 15 days, will grow on the inducing culture explant and transfer carry out enrichment culture on enrichment culture; Cultivate and differentiate clump bud after 15～20 days; Every 15～20 days, the clump bud that breaks up is cut into individual plant inoculates on proliferated culture medium, again differentiation clump bud;
5), strong seedling culture: the clump bud that will differentiate is cut into individual plant and is inoculated on the strong seedling culture base, and the budlet base portion expands into clove after 15～20 days, and diameter is 0.3～0.8 centimetre;
6), culture of rootage: the clove after the strong seedling culture is inoculated on the root media, and the clove base portion bore 2～7 root systems in 10～15 days;
7), the domestication of tissue cultivating seedling and transplanting: when culture of rootage in the time of 20 days, the clove that will take root moves under the normal temperature and adapts to 3～5 days, open the cultivation bottle cap and adapt to 3～5 days again, be transplanted to behind the clean root medium spun yarn is housed: peat: perlitic volume ratio is in the cave dish of 2:1:1 matrix, waters permeable.
The condition of culture in described each tissue culture stage is, culturing room's temperature is 24 ± 2 ℃, and light application time is that 12h/d, intensity of illumination are 25001x～3000Lx.
Embodiment 2: the tissue culture of the red blue stone garlic in the present embodiment 2 and the method for quick propagating technology, carry out according to the following steps:
1), culture medium preparation, comprise that each component and every liter of contained weight of minimal medium and each stage medium of group training is:
(1) minimal medium: MS or 1/2MS, wherein sucrose 20g or 40g/L, agar 8g/L, pH5.8;
(2) inducing culture: MS+TDZ0.4mg/L+ active carbon 2g/L, sucrose 40g;
(3) proliferated culture medium: MS+6-BA5mg/L+NAA1.0mg/L, sucrose 40g;
(4) strong seedling culture base: MS+6-BA2mg/L+NAA0.5mg/L, sucrose 40g;
(5) root media: 1/2MS+KT1.0mg/L+IBA2mg/L+ active carbon 2g/L, sucrose 20g.
2), explant selection and sterilization treatment: select for use the red blue stone garlic bulb of robust growth to carry out sterilization treatment, the explant sampling is placed on for 4～May, and the short-tube lycoris scape was extracted out preceding 2 months; Sterilization treatment is that red blue stone garlic bulb is cleaned, excision root and bulb neck, divest the outside 2 layers of scale of brown stain part and bulb, soaked after 10～20 minutes the flowing water flushing 2 hours with liquid detergent, the back is 75% alcohol disinfecting 1 minute with volume ratio, volume ratio is that 0.1% mercuric chloride solution soaked aseptic water washing 4～5 times 10～15 minutes; After the sterilization treatment bulb cut the height that stays apart from 0.8～1.3 centimetre of bulb basal disc, each bulb is on average to being cut to 4, after with the inside and outside two-layer incision of every branch, every has 3～5 layers of scale;
3), inducing culture: the explant after the sterilization treatment is placed on suck dry moisture on the filter paper of sterilizing, inserts on the inducing culture and carry out inducing culture, explant induction is cultivated after 3 days scale and is expanded and curl, and as seen grain of rice shape projection was arranged between scale after 12～15 days;
4), enrichment culture: inducing culture is after 15 days, will grow on the inducing culture explant and transfer carry out enrichment culture on enrichment culture; Cultivate and differentiate clump bud after 15～20 days; Every 15～20 days, the clump bud that breaks up is cut into individual plant inoculates on proliferated culture medium, again differentiation clump bud;
5), strong seedling culture: the clump bud that will differentiate is cut into individual plant and is inoculated on the strong seedling culture base, and the budlet base portion expands into clove after 15～20 days, and diameter is 0.3～0.8 centimetre;
6), culture of rootage: the clove after the strong seedling culture is inoculated on the root media, and the clove base portion bore 2～7 root systems in 10～15 days;
7), the domestication of tissue cultivating seedling and transplanting: when culture of rootage in the time of 20 days, the clove that will take root moves under the normal temperature and adapts to 3～5 days, open the cultivation bottle cap and adapt to 3～5 days again, be transplanted to behind the clean root medium spun yarn is housed: peat: perlitic volume ratio is in the cave dish of 2:1:1 matrix, waters permeable.
The condition of culture in described each tissue culture stage is, culturing room's temperature is 24 ± 2 ℃, and light application time is that 12h/d, intensity of illumination are 25001x～3000Lx.
Embodiment 3: the tissue culture of the red blue stone garlic in the present embodiment 3 and the method for quick propagating technology, carry out according to the following steps:
1), culture medium preparation, comprise that each component and every liter of contained weight of minimal medium and each stage medium of group training is:
(1) minimal medium: MS or 1/2MS, wherein sucrose 20g or 40g/L, agar 8g/L, pH5.8;
(2) inducing culture: MS+TDZ1.0mg/L+ active carbon 2g/L, sucrose 40g;
(3) proliferated culture medium: MS+6-BA7mg/L+NAA2.0mg/L, sucrose 40g;
(4) strong seedling culture base: MS+6-BA2.5mg/L+NAA0.5mg/L, sucrose 40g;
(5) root media: 1/2MS+KT1.0mg/L+IBA1.5mg/L+ active carbon 2g/L, sucrose 20g.
2), explant selection and sterilization treatment: select for use the red blue stone garlic bulb of robust growth to carry out sterilization treatment, the explant sampling is placed on for 4～May, and the short-tube lycoris scape was extracted out preceding 2 months; Sterilization treatment is that red blue stone garlic bulb is cleaned, excision root and bulb neck, divest the outside 2 layers of scale of brown stain part and bulb, soaked after 10～20 minutes the flowing water flushing 2 hours with liquid detergent, the back is 75% alcohol disinfecting 1 minute with volume ratio, volume ratio is that 0.1% mercuric chloride solution soaked aseptic water washing 4～5 times 10～15 minutes; After the sterilization treatment bulb cut the height that stays apart from 0.8～1.3 centimetre of bulb basal disc, each bulb is on average to being cut to 4, after with the inside and outside two-layer incision of every branch, every has 3～5 layers of scale;
3), inducing culture: the explant after the sterilization treatment is placed on suck dry moisture on the filter paper of sterilizing, inserts on the inducing culture and carry out inducing culture, explant induction is cultivated after 3 days scale and is expanded and curl, and as seen grain of rice shape projection was arranged between scale after 12～15 days;
4), enrichment culture: inducing culture is after 15 days, will grow on the inducing culture explant and transfer carry out enrichment culture on enrichment culture; Cultivate and differentiate clump bud after 15～20 days; Every 15～20 days, the clump bud that breaks up is cut into individual plant inoculates on proliferated culture medium, again differentiation clump bud;
5), strong seedling culture: the clump bud that will differentiate is cut into individual plant and is inoculated on the strong seedling culture base, and the budlet base portion expands into clove after 15～20 days, and diameter is 0.3～0.8 centimetre;
6), culture of rootage: the clove after the strong seedling culture is inoculated on the root media, and the clove base portion bore 2～7 root systems in 10～15 days;
7), the domestication of tissue cultivating seedling and transplanting: when culture of rootage in the time of 20 days, the clove that will take root moves under the normal temperature and adapts to 3～5 days, open the cultivation bottle cap and adapt to 3～5 days again, be transplanted to behind the clean root medium spun yarn is housed: peat: perlitic volume ratio is in the cave dish of 2:1:1 matrix, waters permeable.
The condition of culture in described each tissue culture stage is, culturing room's temperature is 24 ± 2 ℃, and light application time is that 12h/d, intensity of illumination are 25001x～3000Lx.
In addition to the implementation, the present invention can also have other embodiments.All employings are equal to the technical scheme of replacement or equivalent transformation formation, all drop on the protection domain of requirement of the present invention.
Claims (4)
1. the method for the tissue culture of a red blue stone garlic and quick propagating technology, it is characterized in that: this method is carried out according to the following steps:
1), culture medium preparation, comprise that each component and every liter of contained weight of minimal medium and each stage medium of group training is:
(1) minimal medium: MS or 1/2MS, sucrose 20～40g/L wherein, agar 8g/L, pH5.8;
(2) inducing culture: MS+TDZ0.2～1.0mg/L+ active carbon 2g/L;
(3) proliferated culture medium: MS+6-BA3～7mg/L+NAA0.5～2.5mg/L;
(4) strong seedling culture base: MS+6-BA0.5～2.5mg/L+NAA0.5～1.0mg/L;
(5) root media: 1/2MS+KT0.5～2.5mg/L+IBA0.5～2.5mg/L+ active carbon 2g/L;
2), explant selection and sterilization treatment: select for use the red blue stone garlic bulb of robust growth to carry out sterilization treatment, the explant sampling is placed on for 4～May, and the short-tube lycoris scape was extracted out preceding 2 months; After the sterilization treatment bulb cut the height that stays apart from 0.8～1.3 centimetre of bulb basal disc, each bulb is on average to being cut to 4, after with the inside and outside two-layer incision of every branch, every has 3～5 layers of scale;
3), inducing culture: the explant after the sterilization treatment is placed on suck dry moisture on the filter paper of sterilizing, inserts on the inducing culture and carry out inducing culture, explant induction is cultivated after 3 days scale and is expanded and curl, and as seen grain of rice shape projection was arranged between scale after 12～15 days;
4), enrichment culture: inducing culture is after 15 days, will grow on the inducing culture explant and transfer carry out enrichment culture on enrichment culture; Cultivate and differentiate clump bud after 15～20 days; Every 15～20 days, the clump bud that breaks up is cut into individual plant inoculates on proliferated culture medium, again differentiation clump bud;
5), strong seedling culture: the clump bud that will differentiate is cut into individual plant and is inoculated on the strong seedling culture base, and the budlet base portion expands into clove after 15～20 days, and diameter is 0.3～0.8 centimetre;
6), culture of rootage: the clove after the strong seedling culture is inoculated on the root media, and the clove base portion bore 2～7 root systems in 10～15 days;
7), the domestication of tissue cultivating seedling and transplanting: when culture of rootage in the time of 20 days, the clove that will take root moves under the normal temperature and adapts to 3～5 days, open the cultivation bottle cap and adapt to 3～5 days again, be transplanted to behind the clean root medium spun yarn is housed: peat: perlitic volume ratio is in the cave dish of 2:1:1 matrix, waters permeable.
2. the tissue culture of red blue stone garlic according to claim 1 and the method for quick propagating technology, it is characterized in that: described sterilization treatment is that red blue stone garlic bulb is cleaned, excision root and bulb neck, divest the outside 2 layers of scale of brown stain part and bulb, soaked after 10～20 minutes the flowing water flushing 2 hours with liquid detergent, the back is 75% alcohol disinfecting 1 minute with volume ratio, and volume ratio is 0.1% mercuric chloride solution immersion 10～15 minutes, aseptic water washing 4～5 times.
3. the tissue culture of red blue stone garlic according to claim 1 and the method for quick propagating technology is characterized in that: described medium comprises that each component and every liter of contained weight of minimal medium and each stage medium of group training is:
(1) minimal medium: MS or 1/2MS, wherein sucrose 20g or 40g/L, agar 8g/L, pH5.8;
(2) inducing culture: MS+TDZ0.4mg/L+ active carbon 2g/L, sucrose 40g;
(3) proliferated culture medium: MS+6-BA5mg/L+NAA1.0mg/L, sucrose 40g;
(4) strong seedling culture base: MS+6-BA2mg/L+NAA0.5mg/L, sucrose 40g;
(5) root media: 1/2MS+KT1.0mg/L+IBA2mg/L+ active carbon 2g/L, sucrose 20g.
4. according to the tissue culture of claim 1 or 3 described red blue stone garlics and the method for quick propagating technology, it is characterized in that, the condition of culture in described each tissue culture stage is, culturing room's temperature is 24 ± 2 ℃, and light application time is that 12h/d, intensity of illumination are 25001x～3000Lx.
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| CN102144554A (en) * | 2011-01-25 | 2011-08-10 | 江苏九久环境科技有限公司 | Method for producing lycoris by plant tissue culture |
| CN102144554B (en) * | 2011-01-25 | 2012-07-04 | 江苏九久环境科技有限公司 | Method for producing lycoris by plant tissue culture |
| CN102138527A (en) * | 2011-03-25 | 2011-08-03 | 广西相成生物科技有限公司 | Method for culturing tissue culture seedlings of glabrous greenbrier rhizome |
| CN102217540A (en) * | 2011-05-19 | 2011-10-19 | 南京林业大学 | Quick propagation method for lycoris chinensis |
| CN102217540B (en) * | 2011-05-19 | 2012-07-11 | 南京林业大学 | Quick propagation method for lycoris chinensis |
| CN102405841A (en) * | 2011-10-13 | 2012-04-11 | 浙江大学 | Method for inducing callus of lycoris radiate by using flower stalks |
| CN102696479A (en) * | 2012-04-24 | 2012-10-03 | 江苏省中国科学院植物研究所 | Method for propagating stonegarlic quickly and efficiently |
| CN102960243A (en) * | 2012-06-28 | 2013-03-13 | 浙江农林大学 | Method for tissue culture and rapid propagation by basal disc-free scales of Lycoris chinensis |
| CN102972289A (en) * | 2012-06-28 | 2013-03-20 | 浙江农林大学 | Method for tissue culture and rapid propagation by using Lycoris chinensis leaves |
| CN102696488A (en) * | 2012-07-09 | 2012-10-03 | 贵州芊芊园艺新技术发展公司 | Method for rapid propagation of lycoris plant seedlings through hydroponics |
| CN103782787A (en) * | 2014-02-27 | 2014-05-14 | 湖南中医药大学 | GPA planting method of Lycoris aurea |
| CN103782787B (en) * | 2014-02-27 | 2015-07-22 | 湖南中医药大学 | GAP planting method of Lycoris aurea |
| CN105379618A (en) * | 2015-10-20 | 2016-03-09 | 韦丽 | In-vitro rapid propagation method for lycoris radiata |
| CN109329060A (en) * | 2018-11-21 | 2019-02-15 | 江苏省中国科学院植物研究所 | The method for carrying out tissue-culturing rapid propagation as explant to change brocade flower short-tube lycoris plateau |
| CN109618805A (en) * | 2019-02-27 | 2019-04-16 | 湖南春光九汇现代中药有限公司 | A kind of implantation methods of evodia rutaecarpa |
| CN109984039A (en) * | 2019-04-08 | 2019-07-09 | 江苏省中国科学院植物研究所 | A kind of perfume short-tube lycoris method for tissue culture |
| CN109984039B (en) * | 2019-04-08 | 2020-07-28 | 江苏省中国科学院植物研究所 | Lycoris radiata tissue culture method |
| CN110583488A (en) * | 2019-10-24 | 2019-12-20 | 杭州植物园（杭州市园林科学研究院） | Method for establishing tissue culture rapid propagation technical system of new lycoris variety' pink |
| CN112243631A (en) * | 2020-09-14 | 2021-01-22 | 云南省农业科学院花卉研究所 | Method for rapidly breaking dormancy of green flower lily seed bulbs |
| CN112237142A (en) * | 2020-11-02 | 2021-01-19 | 江苏省中国科学院植物研究所 | Tissue culture medium for lycoris, callus culture method and method for establishing lycoris regeneration system |
| CN112237142B (en) * | 2020-11-02 | 2022-04-26 | 江苏省中国科学院植物研究所 | Tissue culture medium for establishing Lycoris chinensis or lycoris aurea regeneration system and method thereof |
| CN112753410A (en) * | 2021-01-13 | 2021-05-07 | 上海科立特农科（集团）有限公司 | Method for improving alkaloid content in lycoris by using exogenous substances |
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