CN101361800A - Traditional Chinese medicine for reducing blood fat and enhancing effect and reducing toxin of statins medicine and preparation method thereof - Google Patents
Traditional Chinese medicine for reducing blood fat and enhancing effect and reducing toxin of statins medicine and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese drug for lowering lipid, improving effects and attenuating functions of statins drugs, which is prepared by raw materials according to the proportions as follows: 1000 portions to 3000 portions of star anise powder, 500 portions to 1000 portions of Seleniotatin red yeast rice and 0.01 portions to 0.05 portions of beta-cyclodextrin inclusion selenium. By the comprehensive action of active ingredients extracted from the star anise powder and Seleniotatin red yeast rice, and the beta-cyclodextrin inclusion selenium, the traditional Chinese drug has the lipid management function for controlling cholesterol synthesis, the function for healing and maintaining blood vessel endothelium so as to ensure youthful blood vessel as well as liver protection, spleen strengthening and stomach nourishing, the functions of eliminating free radicals and enhancing the immune function of organism and the like; the traditional Chinese drug has the functions of improving the functions of and attenuating the statins drugs and can solves the fundamental problems of the origins of cardiovascular and cerebrovascular diseases from many aspects without toxic and side effects, therefore the traditional Chinese drug can be used in a comparatively large dose for a comparatively long time, and a new choice is provided for the treatment of hyperlipemia and cardiovascular and cerebrovascular diseases caused by hyperlipemia and atherosclerosis as well as the control of the occurrence of cardiovascular and cerebrovascular events.
Description
Technical field
The present invention relates to a kind ofly effectively to suppress blood fat rising, reparation and maintenance vascular endothelial function, protect the liver strengthening the spleen and nourishing the stomach, antioxidation and removing people's intravital " free radical " medicine, belong to the field of Chinese medicines.
Background technology
Cardiovascular and cerebrovascular disease is first killer of harm humans healthy life in the present worldwide, only just has every year 3000000 people to die from cardiovascular and cerebrovascular disease in China, people's death was arranged, make number one in average per 10 seconds.The main hazard factor that hyperlipemia is that old people's Incidence of CHD increases, myocardial infarction and patients with coronary heart disease case fatality rate increase.Cardiovascular and cerebrovascular disease has the characteristics of high incidence, high mortality, high disability rate, high relapse rate, and the clinical treatment of cardiovascular and cerebrovascular disease is the scheme that adopts repeated drug taking, SM mostly.Statins drops into clinical use and has opened up the new era of transferring the blood-lipoids medicine, be the most effective since the dawn of human civilization, the safest, the product of the control cardiovascular and cerebrovascular disease that the mechanism of action is the clearest and the most definite, but the accent fat effect of statins exists significantly ageing again, dose-effect relationship, be dose dependent therapeutical effect and dose dependent toxic reaction, common main side effects has: hepatic and renal function injure, rhabdomyolysis, gastrointestinal reaction, dizzy, anaphylaxis (erythra) etc., it is relatively poor to have caused patient to take the compliance of statins thus, is reluctant to accept long-time and heavy dose of active and takes.
Summary of the invention
The objective of the invention is under instruction of Chinese Medicine theory, walk the road that Chinese and western medicine combines, the theory characteristic advantage of Chinese medicine is combined with the effect for reducing fat that above-mentioned existing statins can suppress 3-hydroxy-3-methyl glutaryl coenzyme-A reductase, reach and effectively to be in harmonious proportion lipid composition, can alleviate the toxic and side effects of statins again, further realize removing the lipid components of atheromatous plaque, recover tremulous pulse inner wall smooth and elasticity, guarantee unobstructed blood vessel, remove free radical, all many-sided fundamental issues that solves the cardiovascular and cerebrovascular disease source such as enhancing human body immunity function, and can be heavy dose of and the taking of long period, for delaying and providing of cardiovascular and cerebrovascular vessel incident the has been provided a kind of new blood fat reducing and the Chinese medicine of efficacy enhancing and toxicity reducing statins effect.
Another object of the present invention provides this preparation method of Chinese medicine.
Empirical summary draws according to long-term clinical practice for the apolegamy of the raw medicinal material of the Chinese medicine of blood fat reducing of the present invention and the effect of efficacy enhancing and toxicity reducing statins and consumption thereof, and the main effective ingredient in its raw medicinal material all has curative effect preferably in the following weight parts scope: 1000～3000 parts in Fructus Anisi Stellati powder, 500～1000 parts of statin Monas cuspurpureus Went rich in selenium, 0.01～0.05 part on beta-cyclodextrin inclusion compound selenium.
Preferred weight part scope is: 1000～2000 parts of Fructus Anisi Stellatis, 650～750 parts of statin Monas cuspurpureus Went rich in selenium, 0.02～0.05 part on beta-cyclodextrin inclusion compound selenium.
The preferred for preparation method of the Chinese medicine of blood fat reducing of the present invention and the effect of efficacy enhancing and toxicity reducing statins comprises the following steps:
A), take by weighing each crude drug by weight ratio: Fructus Anisi Stellati, statin Monas cuspurpureus Went rich in selenium, beta-cyclodextrin inclusion compound selenium;
B), the Fructus Anisi Stellati with described weight portion proportioning is ground into fine powder, place microwave container, add 70～90% an amount of ethanol, with transferring in the multi-function extractor behind the microwave assistant alcohol extraction, restock adds 70～90% an amount of ethanol, flooded 3-5 days, continuous backflow is extracted 3-6h, filters, collect filtrate, the ethanol that reclaims in the filtrate is concentrated into nothing alcohol flavor, gets Fructus Anisi Stellati extract after the cooling, and is standby;
C), the statin Monas cuspurpureus Went rich in selenium with described weight proportion is ground into fine powder, repeat lixiviate 2 times through 70～90% ethanol lixiviates, filtration, filtering residue reuse 70～90% ethanol again, merge three times filtrate, the ethanol that reclaims in the filtrate does not extremely have the alcohol flavor, get the statin Monas cuspurpureus Went rich in selenium extract after the cooling, standby;
D), with above-mentioned Fructus Anisi Stellati extract, statin Monas cuspurpureus Went rich in selenium extract and the abundant mixing of beta-cyclodextrin inclusion compound selenium, can be made into solid finished dosage forms such as electuary, powder, granule, tablet, capsule after adding the abundant mixing of film-making adjuvant again, promptly get the Chinese medicine preparation of blood fat reducing and the effect of efficacy enhancing and toxicity reducing statins.
In the above-mentioned preparation method of Chinese medicine, the selenic preparation method of described beta-cyclodextrin inclusion compound comprises the steps: that regulating scale in colloid mill start back puts 240 orders, adds the 0.94kg distilled water earlier, adds beta-schardinger dextrin-1.0kg again, grind and evenly become pasty state, and then add sodium selenite fine powder 0.25kg gradually, continue to grind to form underflow, underflow is placed refrigerator cold-storage after, again through centrifugalize, the collecting precipitation thing places 60～70 ℃ of baking ovens to dry, and gets beta-cyclodextrin inclusion compound selenium.
In the above-mentioned preparation method of Chinese medicine, the preparation method of described statin Monas cuspurpureus Went rich in selenium comprises the following steps:
A), the water of filtering after high quality white rice washed, add 5～10mg Na by every 100g rice
2SeO
3Ratio with Na
2SeO
3Aqueous solution evenly admix in the rice that the solid carbon dioxide of filtering divides, rice rich in selenium;
B), with rice rich in selenium with vapour cooking to ripe and sticking, the inoculum concentration by 5～10% is admixed the mould starter strain of liquid high resistant mutant strain CP-626 lovastatin high yield monascus bacterial strain in the refrigerative rice, must inoculate the rice rich in selenium of lovastatin monascus ruber;
C), inoculation there is the rice rich in selenium of lovastatin monascus ruber place culturing room's fermentation culture, through 6～7 days fermentation culture, make the thalline of the lovastatin monascus ruber after a large amount of breedings contain abundant organic selenium element, place 50-60 ℃ of baking oven to dry again, pick out the grain of rice that is popular in and promptly get statin Monas cuspurpureus Went rich in selenium.
In the above-mentioned preparation method of Chinese medicine, the preparation method of the mould starter strain of described high resistant mutant strain CP-626 lovastatin high yield monascus bacterial strain comprise the steps: to select for use high-quality set out monascus starter strain bacterial strain → inclined-plane inoculation back cultivate one the week → add sterilized water to make and embrace a fullness over the chest during pregnancy liquid, dilute 10 times air-dry in aseptic plate, put the implanter target chamber and carry out the pulse injection, ion energy is 100keV, and injecting nitrogen ion dosage is 1 * 10
14N
+/ cm
2After the mutation, the just change bacterial strain that screens on 10 μ g/mL nystatin resistant panel is the mould starter strain of high resistant mutant strain CP-626 lovastatin high yield monascus bacterial strain.
The Chinese medicine of blood fat reducing of the present invention and the effect of efficacy enhancing and toxicity reducing statins, mainly selecting for use the extract of Fructus Anisi Stellati and rich selenium lovastatin Monas cuspurpureus Went to add three kinds of food of beta-cyclodextrin inclusion compound selenium again is that feedstock production forms, wherein the main component that Fructus Anisi Stellati contained is: many kinds of highly oxidized sesquiterpene lactoness of 3O such as volatile compounds such as anisaldehyde, sikimitoxin and derivant thereof, flavones ingredient, shikimic acid (Shikimic acid) magistery relevant with anti-avian influenza, as monarch drug; The contained main component of rich selenium lovastatin Monas cuspurpureus Went is: the native compound of monascus ruber, lovastatin and other Statins structures and organic selenium element, be ministerial drug can with the monarch drug compatibility, play and set upright and eliminating evil and Gu; Beta-cyclodextrin inclusion compound selenium can be the selenium element that human body provides high activity, high security, not only can increase selenium in gastral absorption, simultaneously also can increase selenium in the intravital bioavailability of people, effect and range of application, so can be used as adjuvant drug, assist a ruler in governing a country Fructus Anisi Stellati, rich selenium lovastatin Monas cuspurpureus Went three medicines share, rational proportion, can mend, can attack, mutual restraint between two drugs, restriction or checking relation in five elements, can play benefiting QI and nourishing blood, warming YANG to expel cold, protect the liver strengthening the spleen and nourishing the stomach, blood circulation promoting and blood stasis dispelling, have the effect of potentiation preferably, detoxifcation for statins.
The Chinese medicine of blood fat reducing of the present invention and the effect of efficacy enhancing and toxicity reducing statins mainly has following remarkable function and outstanding substantive distinguishing features:
1, have and suppress the effect of the synthetic accent fat of cholesterol, promptly have purify the blood, the source transfers the fat effect: as can blood fat reducing, triglyceride reducing, cholesterol reducing, fall LDL-C, fall VLDL-C, fall blood viscosity; Rising HDL-C, rising ApoA-1/ApoB ratio.
2, reparation and maintenance vascular endothelial function make the blood vessel rejuvenation: can suppress the propagation of blood vessel endothelium smooth muscle cell and migration, inflammation-inhibiting, stable and remove atherosclerotic plaque composition in the blood vessel, promote angiogenesis, repair vascular endothelial injury, improve the cardiac-cerebral ischemia state, and should effect than effect for reducing blood fat occur more Zao.
3, has the strengthening the spleen and nourishing the stomach of protecting the liver effect: can play potentiation, fall toxic action for statins; Also can cooperate statins to unite use, make patient also can be used as the auxiliary treatment in the cardiovascular and cerebrovascular diseases that causes by hyperlipemia and atherosclerosis with the statins of taking of heavy dose of and long period.
4, the raw materials used natural food composition that is human three meals in a day, not only can not produce toxic and side effects to vitals such as gastrointestinal tract, heart and brain, Liver and kidney, its effective ingredient can also nutrition and the histiocyte of moist internal organs, be highly advantageous to and improve cardiovascular and cerebrovascular vessel patient's immunologic function, the enhancing body resistance, do not rebound after the healing, can clear up maintenance timely blood vessel.
5, adopting mental retardation (100keV) nitrogen ion beam implantation dosage is 1 * 10
14N
+/ cm
2As the biological induced-mutation source, with low energy ion beam biotechnology be applied to the to set out mutagenic breeding of monascus starter strain, have with space breeding the effect that plays the same tune on different musical instruments is arranged, successfully filter out good high resistant mutant strain CP-626 high-yield selenium-rich lovastatin monascus starter strain bacterial strain, it is stable to have heritability, can produce the Monas cuspurpureus Went that is rich in organic selenium and lovastatin, also a kind of safe, efficient, controlled, economic means are provided, for the probability of exploring the gene loci site directed mutagenesis provides new method for increasing gene bank and cultivating biological new varieties.
6, antioxidation and removing free radical ability are strong: people's intravital " free radical " is the root of all evils, the source of all kinds of diseases and ailments, the world today is because natural environment is seriously polluted, ultraviolet radiation, Bad Eating Habit, disease and antibacterial or virus are invaded, the environment pollution of body metabolism is heavy, make the human lives in the peroxidating environment, multiple factor such as rhythm of life is fast in addition, caused free radical accumulation in vivo to grow with each passing day, and human body is removed the ability and day decline of free radical, and therefore removing the intravital free radical of people timely and effectively is the task of top priority.
Because contain that human body easily absorbs in the medicine of the present invention and the Monas cuspurpureus Went rich in selenium mycete that is easy to extensively utilize in " organic selenium " and beta-cyclodextrin inclusion compound selenium element, can work in coordination with intravital " chemical and biological weapons " that contain selenium polyphenoils enzyme system of people and keep the balance generation and the removing of " free radical " in the body jointly, can reach from effectively preventing cardiovascular and cerebrovascular disease, diabetes, cataract, tumor and infection etc. in essence.Be fit to cerebral thrombosis, cerebral infarction, coronary heart disease, myocardial infarction person, dizziness headache, nervous breathe hard, early symptoms such as feeling of oppression and pain in the chest, numbness of hands and feet, hyperlipidemia and the cardiovascular and cerebrovascular disease patient's that causes by hyperlipidemia and atherosclerosis auxiliary treatment, also be fit to the cardiovascular health-care function of middle-aged and elderly people, patients such as hypertension, diabetes take.
The specific embodiment
Chinese medicine of the present invention can be respectively from bulk drugs of following each group effective component extracting make:
1,1000 parts in Fructus Anisi Stellati powder, 500 parts of statin Monas cuspurpureus Went rich in selenium, 0.01 part on beta-cyclodextrin inclusion compound selenium.
2,1000 parts in Fructus Anisi Stellati powder, 1000 parts of statin Monas cuspurpureus Went rich in selenium, 0.02 part on beta-cyclodextrin inclusion compound selenium.
3,1000 parts in Fructus Anisi Stellati powder, 500 parts of statin Monas cuspurpureus Went rich in selenium, 0.03 part on beta-cyclodextrin inclusion compound selenium.
4,1500 parts in Fructus Anisi Stellati powder, 700 parts of statin Monas cuspurpureus Went rich in selenium, 0.03 part on beta-cyclodextrin inclusion compound selenium.
5,2000 parts in Fructus Anisi Stellati powder, 1000 parts of statin Monas cuspurpureus Went rich in selenium, 0.05 part on beta-cyclodextrin inclusion compound selenium.
6,2500 parts in Fructus Anisi Stellati powder, 500 parts of statin Monas cuspurpureus Went rich in selenium, 0.01 part on beta-cyclodextrin inclusion compound selenium.
7,3000 parts in Fructus Anisi Stellati powder, 1000 parts of statin Monas cuspurpureus Went rich in selenium, 0.05 part on beta-cyclodextrin inclusion compound selenium.
8,3000 parts in Fructus Anisi Stellati powder, 500 parts of statin Monas cuspurpureus Went rich in selenium, 0.01 part on beta-cyclodextrin inclusion compound selenium.
9,3000 parts in Fructus Anisi Stellati powder, 1000 parts of statin Monas cuspurpureus Went rich in selenium, 0.05 part on beta-cyclodextrin inclusion compound selenium.
10,2000 parts in Fructus Anisi Stellati powder, 650 parts of statin Monas cuspurpureus Went rich in selenium, 0.02 part on beta-cyclodextrin inclusion compound selenium.
11,1000 parts in Fructus Anisi Stellati powder, 650 parts of statin Monas cuspurpureus Went rich in selenium, 0.03 part on beta-cyclodextrin inclusion compound selenium.
12,1500 parts in Fructus Anisi Stellati powder, 750 parts of statin Monas cuspurpureus Went rich in selenium, 0.05 part on beta-cyclodextrin inclusion compound selenium.
13,1500 parts in Fructus Anisi Stellati powder, 650 parts of statin Monas cuspurpureus Went rich in selenium, 0.04 part on beta-cyclodextrin inclusion compound selenium.
14,2000 parts in Fructus Anisi Stellati powder, 750 parts of statin Monas cuspurpureus Went rich in selenium, 0.05 part on beta-cyclodextrin inclusion compound selenium.
In sum, bulk drugs all is effective between 0.01～0.05 part in 1000～3000 parts in Fructus Anisi Stellati powder, 500～1000 parts of statin Monas cuspurpureus Went rich in selenium, beta-cyclodextrin inclusion compound selenium.
The preferable range of above-mentioned weight portion crude drug is: 1000～2000 parts in Fructus Anisi Stellati powder, 650～750 parts of statin Monas cuspurpureus Went rich in selenium, 0.02～0.05 part on beta-cyclodextrin inclusion compound selenium.
At first the prescription proportion speed according to above-mentioned preferred three kinds of raw medicinal materials takes by weighing each weight portion, press above-mentioned preparation method again with Fructus Anisi Stellati extract, statin Monas cuspurpureus Went rich in selenium extract and the abundant mixing of beta-cyclodextrin inclusion compound selenium, add film-making adjuvant (as pregelatinized Starch, dextrin, 30 POVIDONE K 30 BP/USP-30, magnesium stearate) again and fully can be made into solid finished dosage forms such as electuary, powder, granule, tablet, capsule behind the mixing, promptly get the Chinese medicine preparation of blood fat reducing and the effect of efficacy enhancing and toxicity reducing statins.
Chinese medicine of the present invention is through 6 months long-term toxicity test for animals result of gastric infusion: the SD rat is divided into three groups, and 30 every group, male and female half and half (being divided into high, medium and low three dosage groups, 10 every group) are wherein given normal saline through irritating stomach for first group; Second group is given the dosage of " Chinese medicine of blood fat reducing of the present invention and efficacy enhancing and toxicity reducing statins " through the filling stomach is 0.25g, 2.5g and 12.5g/kg.d; The 3rd group is given the dosage of lovastatin through the filling stomach is 0.5mg, 5.0mg and 50.0mg/kg.d.During administration, the indexs such as clinical manifestation, hematology, blood biochemistry, organ coefficient and histopathology of observing respectively after each treated animal administration are carried out the evaluation of drug toxicity.Result of study: main toxic reaction shows lovastatin 50mg/kg.d and 5.0mg group, shows after the administration that reaction and symptoms such as sialorrhea, hair are fluffy, busy, diarrhoea, step have appearred in the SD rat, total food ration with lose weight; Blood test shows that total white blood cells increases to some extent, erythrocyte sum, content of hemoglobin and platelet count obviously descend (P all＜0.05); Blood biochemistry checking shows that ALT, AST, ALP and CK raise 1～8 times (P all＜0.05), and creatinine and blood urea nitrogen raise (P all＜0.05) to some extent, but do not cause animal dead; Histopathological analysis result shows that lovastatin 50mg/kg.d group has occurred and Liver and kidney pathological change like the physiognomy.And similar reaction does not all appear in normal saline group and " Chinese medicine of blood fat reducing of the present invention and efficacy enhancing and toxicity reducing statins " 0.25g, 2.5g and three dosage groups of 12.5g/kg.d and lovastatin 0.5mg/kg.d dosage group during whole administration, at the convalescent period viewing duration, the symptom that each treated animal of reaction the occurs recovery normal condition that fades away.As seen " Chinese medicine of blood fat reducing of the present invention and efficacy enhancing and toxicity reducing statins " not only can reduce the toxic and side effects of lovastatin, and can make the clinical non-toxic of lovastatin increase to 5～25 times (P all＜0.01).
The result of the clinical trial of Chinese medicine of the present invention: test objective: observe the clinical efficacy that the over-65s gerontal patient takes " Chinese medicine of blood fat reducing of the present invention and efficacy enhancing and toxicity reducing statins " and lovastatin tablet treatment coronary heart disease merging hyperlipidemia patient respectively, inquire into the characteristics and the corresponding atherosclerosis firsts and seconds preventive measure of lipid lowering therapy.Method: 138 routine over-65s are suffered from coronary heart disease merging hyperlipidemia patient be divided into " Chinese medicine of blood fat reducing of the present invention and efficacy enhancing and toxicity reducing statins " treatment group 4.0g/d and lovastatin treatment group 20mg/d at random; Treating for 8 weeks or measuring the Blood Lipid level that it treats front and back more than 8 weeks respectively; Each group is divided into two groups according to degree of danger again, and promptly dangerous group and atheromatosis or diabetic groups are observed its therapeutic effect.The result: the cholesterol (TC) before and after the lovastatin treatment group treatment, triglyceride (TG), low-density lipoprotein cholesterol (LDL-C) level has obvious decline (P＜0.05), and HDL-C level raises to some extent, but diversity not significantly (P〉0.05); (LDL-C) level all has very obviously decline (P＜0.01), and HDL-C level has obvious rising (P＜0.05) for cholesterol (TC) before and after the treatment of " Chinese medicine of blood fat reducing of the present invention and efficacy enhancing and toxicity reducing statins " treatment group, triglyceride (TG), low-density lipoprotein cholesterol.Total compliance rate is 77.06% in 138 examples, wherein dangerous group compliance rate is 88.01%, atheromatosis or diabetic groups compliance rate are 54.02%, the lovastatin treatment is for atherosclerosis or diabetics blood fat reducing in secondary prevention relatively difficulty up to standard, and total blood fat compliance rate of " Chinese medicine of blood fat reducing of the present invention and efficacy enhancing and toxicity reducing statins " treatment group is 68.81%, apparently higher than 36.61% (P＜0.05) of lovastatin group; Conclusion: " Chinese medicine of blood fat reducing of the present invention and the effect of efficacy enhancing and toxicity reducing statins " and lovastatin are for reducing senile hyperlipidemia, treatment atherosclerosis and diabetes all have better curative effect, the clinical efficacy of " but the Chinese medicine of blood fat reducing of the present invention and the effect of efficacy enhancing and toxicity reducing statins " treatment group will obviously be better than lovastatin treatment group, illustrate that the Chinese medicine ingredients in " Chinese medicine of blood fat reducing of the present invention and the effect of efficacy enhancing and toxicity reducing statins " has certain potentiation for statins, Attenuation has proved that also " Chinese medicine of blood fat reducing of the present invention and efficacy enhancing and toxicity reducing statins " can be used as the first-selected kind of blood fat reducing treatment up to standard for atherosclerosis or diabetics in the secondary prevention process simultaneously.
Chinese medicine of the present invention is fit to cerebral thrombosis, cerebral infarction, coronary heart disease, myocardial infarction person, dizziness headache, nervous breathe hard, early symptoms such as feeling of oppression and pain in the chest, numbness of hands and feet, hyperlipidemia and the cardiovascular and cerebrovascular disease patient's that causes by hyperlipidemia and atherosclerosis auxiliary treatment, also be fit to the cardiovascular health-care function of middle-aged and elderly people, patients such as hypertension, diabetes take.
Claims (8)
1, the Chinese medicine of a kind of blood fat reducing and the effect of efficacy enhancing and toxicity reducing statins is characterized in that making that main medicine in the raw material of this pharmaceutically active ingredient is formed and weight portion is: 1000～3000 parts in Fructus Anisi Stellati powder, 500～1000 parts of statin Monas cuspurpureus Went rich in selenium, 0.01～0.05 part on beta-cyclodextrin inclusion compound selenium.
2, the Chinese medicine of blood fat reducing as claimed in claim 1 and the effect of efficacy enhancing and toxicity reducing statins, the consumption that it is characterized in that making the raw material of this pharmaceutically active ingredient is 1000～2000 parts of Fructus Anisi Stellatis, 650～750 parts of statin Monas cuspurpureus Went rich in selenium, 0.02～0.05 part on beta-cyclodextrin inclusion compound selenium.
3, claim 1 or 2 described preparation method of Chinese medicine is characterized in that comprising the following steps:
A), take by weighing each materials of weight proportions medicine: Fructus Anisi Stellati, statin Monas cuspurpureus Went rich in selenium, beta-cyclodextrin inclusion compound selenium;
B), the Fructus Anisi Stellati with described weight proportion is ground into fine powder, place microwave container, add 70～90% an amount of ethanol, with transferring in the multi-function extractor behind the microwave assistant alcohol extraction, restock adds 70～90% an amount of ethanol, flooded 3-5 days, continuous backflow is extracted 3-6h, filters, collect filtrate, the ethanol that reclaims in the filtrate is concentrated into nothing alcohol flavor, gets Fructus Anisi Stellati extract after the cooling, and is standby;
C), the statin Monas cuspurpureus Went rich in selenium with described weight proportion is ground into fine powder, repeat lixiviate 2 times through 70～90% ethanol lixiviates, filtration, filtering residue reuse 70～90% ethanol again, merge three times filtrate, the ethanol that reclaims in the filtrate does not extremely have the alcohol flavor, get the statin Monas cuspurpureus Went rich in selenium extract after the cooling, standby;
D), with above-mentioned Fructus Anisi Stellati extract, statin Monas cuspurpureus Went rich in selenium extract and the abundant mixing of beta-cyclodextrin inclusion compound selenium, can be made into solid finished dosage forms such as electuary, powder, granule, tablet, capsule after adding the abundant mixing of film-making adjuvant again, promptly get the Chinese medicine preparation of blood fat reducing and the effect of efficacy enhancing and toxicity reducing statins.
4, as preparation method of Chinese medicine as described in the claim 3, it is characterized in that the selenic preparation method of described beta-cyclodextrin inclusion compound comprises the steps: that regulating scale in colloid mill start back puts 240 orders, add the 0.94kg distilled water earlier, add beta-schardinger dextrin-1.0kg again, grind and evenly become pasty state, and then add sodium selenite fine powder 0.25kg gradually, continue to grind to form underflow, after underflow placed refrigerator cold-storage, again through centrifugalize, the collecting precipitation thing places 60～70 ℃ of baking ovens to dry, and gets beta-cyclodextrin inclusion compound selenium.
5, as preparation method of Chinese medicine as described in the claim 3, it is characterized in that the preparation method of described statin Monas cuspurpureus Went rich in selenium comprises the following steps:
A), high quality white rice is washed the water of filtering, by every 100g rice adding 5～10mg Na
2SeO
3Ratio with Na
2SeO
3Aqueous solution evenly admix in the rice of the solid carbon dioxide part of filtering, rice rich in selenium;
B), rice rich in selenium is extremely ripe and sticking with vapour cooking, inoculum concentration by 5～10% mixes the liquid mould starter strain of high resistant mutant strain CP-626 lovastatin high yield monascus bacterial strain through mental retardation nitrogen ion beam injection mutation to refrigerative rice, must inoculate the rice rich in selenium of lovastatin monascus ruber;
C), inoculation there is the rice rich in selenium of lovastatin monascus ruber place culturing room's fermentation culture, through 6～7 days fermentation culture, make the thalline of the lovastatin monascus ruber after a large amount of breedings contain abundant organic selenium element, after placing 50～60 ℃ of baking oven oven dry, pick out the grain of rice that is popular in, promptly get statin Monas cuspurpureus Went rich in selenium.
6, as preparation method of Chinese medicine as described in the claim 5, the preparation method that it is characterized in that the mould starter strain of described high resistant mutant strain CP-626 lovastatin high yield monascus bacterial strain comprise the steps: to select for use high-quality set out monascus starter strain bacterial strain → inoculation back, inclined-plane cultivate one the week → add sterilized water to make and embrace a fullness over the chest during pregnancy liquid, dilute 10 times air-dry in aseptic plate, put the implanter target chamber and carry out the pulse injection, selecting ion energy is 100keV, and injecting nitrogen ion dosage is 1 * 10
14N
+/ cm
2After the mutation, the just change bacterial strain that screens on 10 μ g/mL nystatin resistant panel is the mould starter strain of high resistant mutant strain CP-626 lovastatin high yield monascus bacterial strain.
7, as preparation method of Chinese medicine as described in the claim 4, it is characterized in that the preparation method of described statin Monas cuspurpureus Went rich in selenium comprises the following steps:
A), high quality white rice is washed the water of filtering, by every 100g rice adding 5～10mg Na
2SeO
3Ratio with Na
2SeO
3Aqueous solution evenly admix in the rice of the solid carbon dioxide part of filtering, rice rich in selenium;
B), rice rich in selenium is extremely ripe and sticking with vapour cooking, inoculum concentration by 5～10% mixes the liquid mould starter strain of high resistant mutant strain CP-626 lovastatin high yield monascus bacterial strain through mental retardation nitrogen ion beam injection mutation to refrigerative rice, must inoculate the rice rich in selenium of lovastatin monascus ruber;
C), inoculation there is the rice rich in selenium of lovastatin monascus ruber place culturing room's fermentation culture, through 6～7 days fermentation culture, make the thalline of the lovastatin monascus ruber after a large amount of breedings contain abundant organic selenium element, after placing 50～60 ℃ of baking oven oven dry, pick out the grain of rice that is popular in, promptly get statin Monas cuspurpureus Went rich in selenium.
8, as preparation method of Chinese medicine as described in the claim 7, the preparation method that it is characterized in that the mould starter strain of described high resistant mutant strain CP-626 lovastatin high yield monascus bacterial strain comprise the steps: to select for use high-quality set out monascus starter strain bacterial strain → inoculation back, inclined-plane cultivate one the week → add sterilized water to make and embrace a fullness over the chest during pregnancy liquid, dilute 10 times air-dry in aseptic plate, put the implanter target chamber and carry out the pulse injection, selecting ion energy is 100keV, and injecting nitrogen ion dosage is 1 * 10
14N
+/ cm
2After the mutation, the just change bacterial strain that screens on 10 μ g/mL nystatin resistant panel is the mould starter strain of high resistant mutant strain CP-626 lovastatin high yield monascus bacterial strain.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972833A (en) * | 2012-11-26 | 2013-03-20 | 郭纯 | Lipid reducing red yeast rice bagged beverage and preparation method thereof |
| CN104706962A (en) * | 2015-04-02 | 2015-06-17 | 王继波 | Traditional Chinese medicine composite for treating spleen deficiency and dampness-heat type hyperlipidemia |
| CN106800508A (en) * | 2017-01-14 | 2017-06-06 | 华北理工大学 | New benzoic acid derivative and preparation method thereof in wild anise, using and pharmaceutical composition |
| CN108379594A (en) * | 2018-04-30 | 2018-08-10 | 韩长平 | A kind of cyclodextrin inclusion compound and preparation method thereof |
-
2008
- 2008-09-23 CN CNA2008101570613A patent/CN101361800A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102972833A (en) * | 2012-11-26 | 2013-03-20 | 郭纯 | Lipid reducing red yeast rice bagged beverage and preparation method thereof |
| CN104706962A (en) * | 2015-04-02 | 2015-06-17 | 王继波 | Traditional Chinese medicine composite for treating spleen deficiency and dampness-heat type hyperlipidemia |
| CN106800508A (en) * | 2017-01-14 | 2017-06-06 | 华北理工大学 | New benzoic acid derivative and preparation method thereof in wild anise, using and pharmaceutical composition |
| CN106800508B (en) * | 2017-01-14 | 2019-03-12 | 华北理工大学 | New benzoic acid derivative and preparation method thereof, application and pharmaceutical composition in wild illiciumverum |
| CN108379594A (en) * | 2018-04-30 | 2018-08-10 | 韩长平 | A kind of cyclodextrin inclusion compound and preparation method thereof |
| CN108379594B (en) * | 2018-04-30 | 2020-02-14 | 韩长平 | Cyclodextrin inclusion compound and preparation method thereof |
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