CN101347523A - Microsphere for injuecting 'Shuang Hualian' for treating viral disease of bird and preparation thereof - Google Patents
Microsphere for injuecting 'Shuang Hualian' for treating viral disease of bird and preparation thereof Download PDFInfo
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Abstract
The invention relates to a microsphere used for injecting Shuanghuanglian which is used for treating poultry viral diseases and a preparation method thereof; the microsphere consists of 2-60% of Chinese medicinal material extracts which are taken as active ingredients and 39-95% of biodegradable medicinal high-molecular excipients which have microsphere weight and have molecular weight ranging from 4,000 to 9,000,000 by weight proportion; wherein, the weight percentage of medicinal materials is as follows: 13-40% of Chinese goldthread, 15-45% of honeysuckle, 25-61% of forsythia and 11-40% of milk veteh. The medicines are prepared into microspheres by emulsifying dispersion method. The beneficial effects of the invention is that: (1) developing a new medicine preparation for preventing and treating poultry viral diseases and increasing disease resistance of the body; (2) reasonable prescription and strong antiviral ability; (3) advanced technique, stable preparation and long storage time; (4) broad medicine sources and no toxicity and side effect, little drug resistance generated and meeting the international sanitary inspection requirements. Especially, the microsphere has significant effect on the treatment of poultry infectious bursal disease, untypical Newcastle disease, infective bronchitis, etc.
Description
Technical field
The present invention relates to treat the field of medicaments of Animal diseases, particularly a kind of 'Shuang Hualian ' injection microsphere and preparation method thereof for the treatment of avian viral diseases.
Background technology
At present, usually based on antibiotic therapy, so not only toxic and side effects is bigger, as the hepatic and renal function to birds certain side effect is arranged, and easily causes the birds drug disposition residual aspect the treatment avian viral diseases; Simultaneously also with environmental pollution, and medical expense is higher.
The Radix Astragali is the dry root of leguminous plant Radix Astagali or Radix Astragali.Its Main Ingredients and Appearance is Russia's class, polysaccharide, aminoacid and trace element.Have enhancing human body immunity power, diuresis, defying age, protect the liver, effect such as blood pressure lowering, heart tonifying, expansion coronary vasodilator and whole body peripheral vessel, anti-mattress, be one of Chinese medicine of using always.
Motherland's medical science thinks that the Radix Astragali both can be used as medicine separately, also can unite use with other medical material, to reach clinical required curative effect.SHUANGHUANLIAN is a kind of Chinese medicine preparation, and Main Ingredients and Appearance is Flos Lonicerae, Rhizoma Coptidis, Fructus Forsythiae.Flos Lonicerae is exactly a Flos Lonicerae, but sweet in the mouth cold in nature, abnormal smells from the patient awns perfume (or spice), both fresh breeze using warming therapy heat can be separated the poison in the blood again, are applicable to epidemic febrile disease heating, macule, pharyngalgia.The Rhizoma Coptidis bitter in the mouth is cold in nature, in the removing pathogen in the heart after the fire, damp and hot in the bowel relieving, be applicable to excessive fire in heart, damp and hot, the damp and hot pestilent toxicity of the intestines and stomach.Fructus Forsythiae bitter in the mouth cool in nature, clearing heart fire goes the various heat symptom-complexs of the part of the body cavity above the diaphragm housing the heart and lungs, is applicable to epidemic febrile disease from the beginning of, headache, heating, excessive thirst, and the carbuncle skin ulcer that pyretic toxicity pent-up causes swells and ache, erysipelas, macule etc.
If adopt raw material of Chinese medicine to make tablet, powder etc., because the interior half-life of the Chinese crude drug water-soluble active ingredient body that has is shorter, need frequent drug administration, therefore use inconvenience; Assimilation effect is not good enough simultaneously, and therapeutic effect is not ideal enough.
Therefore, how providing a kind of prevention effect is remarkable, technology is simply treated avian viral diseases 'Shuang Hualian ' injection with microsphere and preparation method thereof, is one of this technical field scientific research personnel new problem of being badly in need of developing.
Summary of the invention
The object of the invention is to provide the 'Shuang Hualian ' injection microsphere of the treatment avian viral diseases of a kind of brand-new slow release, high bioavailability.
Another object of the present invention is to provide the preparation method of the 'Shuang Hualian ' injection of this treatment avian viral diseases with microsphere.
The present invention is achieved through the following technical solutions:
A kind of 'Shuang Hualian ' injection microsphere for the treatment of avian viral diseases, it is characterized in that comprising that 2～60% the extract by Chinese crude drug is that 39～95% molecular weight ranges of active component and microsphere weight is 4,000～9000, biodegradable medicinal high polymer adjuvant between 000 is formed, the composition of its Chinese crude drug % proportioning by weight is: Rhizoma Coptidis 13%～40%, Flos Lonicerae 15%～45%, Fructus Forsythiae 25%～61%, the Radix Astragali 11%～40%.
The preferred weight percentage composition of described active component crude drug is: Rhizoma Coptidis 24.7%, Flos Lonicerae 26.7%, Fructus Forsythiae 26.3%, the Radix Astragali 22.3%.
Described medicinal high polymer adjuvant is selected from polylactide-co-glycolide, polylactic acid, polyglycolic acid, poly--the 3-butyric ester, polylactic acid-polyglycolic acid, polylactic acid. glycolic, poly-adjacent ester, polylactone, polyanhydride, poly butyric ester-hydroxyl pentanoate copolymer, polyglycolic acid, polypropylene glucosan, hydroxyacetic acid, polylactic acid-polyglycol, polyglycolic acid. and Polyethylene Glycol one or more mixing wherein.
Excellent polylactide-co-glycolide, polylactic acid, the polylactic acid of being selected from of described medicinal high polymer adjuvant. one or more mixing wherein of polyglycolic acid, polylactic acid-glycollic acid, poly butyric ester-hydroxyl pentanoate copolymer.
Described medicinal high polymer adjuvant is a polylactide-co-glycolide; Its molecular weight of described polylactide-co-glycolide is between 9000～13000 dalton; Lactide and Acetic acid, hydroxy-, bimol. cyclic ester polymerization ratio are between 40: 60～60: 40 in the polylactide-co-glycolide.
The said medicine composition proportion by the weight % proportioning of use crude drug, be made into preparation need to described Rhizoma Coptidis, Flos Lonicerae, Fructus Forsythiae, the Radix Astragali etc. further separately or mixed extraction be processed into extract and make gel film with suitable adjuvant.Extracting method wherein generally includes: water extraction method, decoction and alcohol sedimentation technique or microwave extraction method etc.For the ease of understanding, the existing extracting method in present technique field is described below for example:
Rhizoma Coptidis microwave extraction method: get after Rhizoma Coptidis pulverizes, the flask of setting fire, and this flask put into microwave reactor, alcohol reflux is adjusted microwave power and is extracted, and collects extracting solution, Rhizoma Coptidis extract.
The Flos Lonicerae water extraction: the extracting honeysuckle medical material, 20 mesh sieves are pulverized, twice of extracting in water, the water logging bubble that adds for the first time 9～10 times of amounts, heating extraction 1.5 hours adds 5～7 times of water gagings, heating extraction 1 hour for the second time, merge extractive liquid,, suitably concentrate, add 95% ethanol, make to contain alcohol amount arrival 50-70%, concentrate and reclaim ethanol, get Flos Lonicerae extract.
The ethanol extraction method of Fructus Forsythiae: get the Fructus Forsythiae medical material, 20 mesh sieves are pulverized, and add the ethanol extraction three times that 7-9 doubly measures 50% concentration, reflux 8 hours, merge extractive liquid, concentrates and reclaims ethanol, Fructus Forsythiae extract.
The Radix Astragali water extraction alcohol sedimentation method: get the Radix Astragali, 20 mesh sieves are pulverized, and extracting in water filters, suitably concentrate the back and adopt ethanol to precipitate, precipitate with ethanol again after supernatant suitably concentrates, supernatant reclaims ethanol, concentrate Radix Astragali extract.
Except that the independent extraction of above-mentioned medical material, described Rhizoma Coptidis, Flos Lonicerae, all right mixed extraction of Fructus Forsythiae are mainly: Rhizoma Coptidis, Flos Lonicerae, the Fructus Forsythiae medical material of learning from else's experience and pulverizing, and boiling water boils to be carried, filter, merging filtrate, and filtrate is suitably concentrated; Add ethanol and carry out precipitate with ethanol in concentrated solution, leave standstill, supernatant reclaims ethanol, is condensed into extractum, gets medicinal substances extract.
'Shuang Hualian ' injection microsphere diameter of the present invention is 1～250 μ m, and 4,000～9000, the biodegradable medicinal high polymer adjuvant between 000 is formed by the molecular weight ranges of microsphere gross weight 39～95%.
Microsphere of the present invention can adopt the conventional preparation method of microsphere to make, promptly adopt the emulsifying dispersion method to make, implementation step comprises: use dichloromethane, chloroform, ethyl acetate, dioxane, ether, acetone, oxolane, glacial acetic acid and the mixed acid be made up of them are medicinal substances extract and the dissolving of biodegradable pharmaceutic adjuvant, wherein the bulking value percent of pharmaceutic adjuvant in organic solvent is 1～30%, the emulsifying agent that organic facies adopts is the nonionic emulsifier of HLB=4.5～6.0, use polyvinyl alcohol, polyvinyl pyrrolidone, consumption are 0.01～12% of organic facies; Sodium polymethacrylate, sodium polyacrylate, sodium carboxymethyl cellulose solution in addition, prepare continuous water, wherein they are 0.01～10.0 in the percetage by weight of aqueous phase, the emulsifying agent of aqueous phase is the nonionic emulsifier of HLB=14.0～15.5, consumption is 0.01～12% of a water, and the volume ratio of organic facies and water is 1: 4～100; Decentralized photo slowly is injected in the continuous phase by tubule, fully emulsified after, the formed microsphere of sieving separating is drying to obtain.
SHUANGHUANLIAN microsphere of the present invention be will make the microsphere sterilized powder earlier in aseptic 0.9% normal saline solvent suspendible evenly the back intramuscular administration treat.The drug release process of most of conventional dosage forms is all undertaken by first order kinetics, and the blood drug level of its medicine might be lower than minimum effective blood drug concentration and hold time short shortcoming in treatment concentration.For reaching therapeutic purposes and reducing side effect and often take repeatedly medicining mode, the compliance that makes the patient take medicine like this descends.Slow releasing preparation has overcome the deficiency of conventional formulation just, and steady, persistent blood drug level is provided.
The invention has the beneficial effects as follows: Rhizoma Coptidis, Flos Lonicerae, Fructus Forsythiae, Milkvetch Root are extracted the back make microsphere, can reach non-lentamente constant release, reduce and take number of times, improve the purpose of patient's compliance with suitable adjuvant.The extraction process of uses advanced is separated effective monomer in the medical material, extracts the back and makes microsphere with suitable adjuvant.Effective ingredient in the Chinese herbal medicine is slowly discharged.The effective ingredient that extracts in the prescription is the formation of blocking virus nucleic acid directly, suppress duplicating and breeding of virus, but and the humoral immunization of enhancing body and cellular immunization, this invention medicine has the body of inducing generation interferon simultaneously, enhancing antibody forms, strengthen the phagocytosis of macrophage to pathogen, thus effective kill virus.
Medicine of the present invention is external to have direct killing action to bursal disease virus of chickens; Model to artificial challenge's bursal disease virus of chickens also has significant protection and therapeutical effect.Clinical research confirmation medicine of the present invention has excellent curative to bursal disease virus of chickens, especially can remove heating that viral infection causes rapidly, improve symptom, promote sick fowl to recover to search for food, promote laying rate and egg product matter to recover, second day clinical symptoms alleviated rapidly after the general medication, recovery from illness in 3～5 days, the curative effect highly significant.
This medicine is human body immunity improving power effectively, and anti-virus ability is strong, the clinical viral diseases such as treatment poultry infectious bursal disease, atypical newcastle disease, infectious bronchitis that are mainly used in.Reasonable recipe, the variation of heat clearing away approach has taken into full account the thermotaxic characteristics of birds, and the pestilence of dispelling the heat, dispel alleviates the sense of discomfort of body, thereby alleviates the symptom of disease to a great extent, increases disease fowl appetite, promotes the rehabilitation of disease fowl.
Be summarized as follows:
(1) goes out a kind of novel formulation of preventing and treating avian viral diseases, improving body disease-resistant power for clinical development.
(2) reasonable recipe, the absorption rate height, the slow release lasting medicine, anti-virus ability is strong, and selected medicine is the efficient medicine of treatment avian viral diseases.
(3) technology advanced person, preparation is simple, preparation stabilization, lasting medicine, storage period is long.
(4) medicament sources is extensive, cost is low, safely, have no side effect, is difficult for producing drug resistance, meets the international hygiene touchstone.It is remarkable to be used in particular for treating effects such as birds infectious bursal disease, atypical newcastle disease, infectious bronchitis.
The specific embodiment
Below in conjunction with preferred embodiment, to details are as follows according to the specific embodiment provided by the invention:
Embodiment 1
Get Rhizoma Coptidis 247g, Flos Lonicerae 267g, Fructus Forsythiae 263g, Radix Astragali 223g will be through ground honeysuckle, Fructus Forsythiae, and decocting boils 2 times, add 7 times of water gagings at every turn, each 2 hours, merge decoction liquor, filter, filtrate is concentrated into 900ml, adds 95% ethanol, make determining alcohol reach 70%, left standstill 12～24 hours, and filtered, reclaim ethanol; Continue to be condensed into the Flos Lonicerae Fructus Forsythiae extract; To decoct with water 2 times through the Radix Astragali of pulverizing, add 6 times of water gagings at every turn, extracted successively 2 hours, 1 hour, merging filtrate is when being concentrated into the 1500ml left and right sides, adding 95% ethanol, to make determining alcohol be 60%, left standstill 12～24 hours, filters, filtrate recycling ethanol, when being concentrated into the 400ml left and right sides, adding 95% ethanol, to make determining alcohol be 80%, left standstill 12～24 hours, filter, filtrate recycling ethanol, and continue to be condensed into Radix Astragali extract; Add 5 times of water gagings by measuring Rhizoma Coptidis, reflux, extract, 5 hours is collected volatile oil.
Comprise that 2～60% the extract by Chinese crude drug is that 39～95% molecular weight ranges of active component and microsphere weight is 4,000～9000, biodegradable medicinal high polymer adjuvant between 000 dalton is formed, and described medicinal high polymer adjuvant is selected from polylactide-co-glycolide, polylactic acid, polyglycolic acid, poly--the 3-butyric ester, polylactic acid-polyglycolic acid, polylactic acid. glycolic, poly-adjacent ester, polylactone, polyanhydride, poly butyric ester-hydroxyl pentanoate copolymer, polyglycolic acid, the polypropylene glucosan, hydroxyacetic acid, polylactic acid-polyglycol, polyglycolic acid. Polyethylene Glycol one or more mixing wherein.
The preparation of microsphere: above-mentioned 100mg medicinal substances extract, 200mg polylactide-co-glycolide are dissolved in the 3ml dichloromethane, select its molecular weight of polylactide-co-glycolide between 9000～13000 dalton; Lactide and Acetic acid, hydroxy-, bimol. cyclic ester polymerization ratio are between 40: 60～60: 40 in the polylactide-co-glycolide; With syringe under high degree of agitation with it to being equipped with in the three-necked bottle of 10%PVA that 300ml includes the 3.5Ghlb=14 emulsifying agent, after fully emulsified 1 hour, under the pressure of 30 ℃ of 0.03MPa, solvent flashing 1.5 hours, centrifugalize, wash reactant three times with distillation, in 30 ℃ of vacuum drying ovens, dry, 30 ℃ of sterilizations 48 hours, guarantee that residual ethylene oxide content is below 5ppm with oxirane, fill adopts the emulsifying dispersion method to make microsphere; Record particle diameter 1～50 μ m, mean diameter 13.26 μ m, sterilization, packing.
Embodiment 2
Get Rhizoma Coptidis 149g, Flos Lonicerae 422g, Fructus Forsythiae 264g, Radix Astragali 165g will be through ground honeysuckle, Fructus Forsythiae, and decocting boils 2 times, add 7 times of water gagings at every turn, each 2 hours, merge decoction liquor, filter, filtrate is concentrated into 900ml, adds 95% ethanol, make determining alcohol reach 70%, left standstill 12～24 hours, and filtered, reclaim ethanol; Continue to be condensed into the Flos Lonicerae Fructus Forsythiae extract; To decoct with water 2 times through the Radix Astragali of pulverizing, add 6 times of water gagings at every turn, extracted successively 2 hours, 1 hour, merging filtrate is when being concentrated into the 1500ml left and right sides, adding 95% ethanol, to make determining alcohol be 60%, left standstill 12～24 hours, filters, filtrate recycling ethanol, when being concentrated into the 400ml left and right sides, adding 95% ethanol, to make determining alcohol be 80%, left standstill 12～24 hours, filter, filtrate recycling ethanol, and continue to be condensed into Radix Astragali extract; Add 5 times of water gagings by measuring Rhizoma Coptidis, reflux, extract, 5 hours is collected volatile oil.
The preparation of microsphere: with above-mentioned 100mg medicinal substances extract, the 200mg polylactide-co-glycolide is dissolved in the 3ml dichloromethane, with syringe under high degree of agitation with it to being equipped with in the three-necked bottle of 10%PVA that 300ml includes the 3.5Ghlb=14 emulsifying agent, after fully emulsified 1 hour, under the pressure of 30 ℃ of 0.03MPa, solvent flashing 1.5 hours, centrifugalize, wash reactant three times with distillation, in 30 ℃ of vacuum drying ovens, dry, sterilized 48 hours at 30 ℃ with oxirane, guarantee residual ethylene oxide content below 5ppm, fill adopts the emulsifying dispersion method to make microsphere; Record particle diameter 1～50 μ m, mean diameter 13.26 μ m, sterilization, packing.
Other is with embodiment 1.
Embodiment 3
Get Rhizoma Coptidis 331g, Flos Lonicerae 165g, Fructus Forsythiae 312g, Radix Astragali 192g will be through ground honeysuckle, Fructus Forsythiae, and decocting boils 2 times, add 7 times of water gagings at every turn, each 2 hours, merge decoction liquor, filter, filtrate is concentrated into 900ml, adds 95% ethanol, make determining alcohol reach 70%, left standstill 12～24 hours, and filtered, reclaim ethanol; Continue to be condensed into the Flos Lonicerae Fructus Forsythiae extract; To decoct with water 2 times through the Radix Astragali of pulverizing, add 6 times of water gagings at every turn, extracted successively 2 hours, 1 hour, merging filtrate is when being concentrated into the 1500ml left and right sides, adding 95% ethanol, to make determining alcohol be 60%, left standstill 12～24 hours, filters, filtrate recycling ethanol, when being concentrated into the 400ml left and right sides, adding 95% ethanol, to make determining alcohol be 80%, left standstill 12～24 hours, filter, filtrate recycling ethanol, and continue to be condensed into Radix Astragali extract; Add 5 times of water gagings by measuring Rhizoma Coptidis, reflux, extract, 5 hours is collected volatile oil.
The preparation of microsphere: with above-mentioned 100mg medicinal substances extract, the 200mg polylactide-co-glycolide is dissolved in the 3ml dichloromethane, with syringe under high degree of agitation with it to being equipped with in the three-necked bottle of 10%PVA that 300ml includes the 3.5Ghlb=14 emulsifying agent, after fully emulsified 1 hour, under the pressure of 30 ℃ of 0.03MPa, solvent flashing 1.5 hours, centrifugalize, wash reactant three times with distillation, in 30 ℃ of vacuum drying ovens, dry, sterilized 48 hours at 30 ℃ with oxirane, guarantee residual ethylene oxide content below 5ppm, fill adopts the emulsifying dispersion method to make microsphere; Record particle diameter 1～50 μ m, mean diameter 13.26 μ m, sterilization, packing.
Other is with embodiment 1.
Application example
200 25 age in days broiler (aseptic through physical examination) are divided into totally 5 groups of A, B, C, D, E, test behind B, C, the strong malicious counteracting toxic substances of D, E group process artificial challenge's 0.5ml bursal disease virus of chickens.A group is healthy group, infective virus not, not administration; B organizes negative control group, infective virus, not administration; The C group is the pharmaceutical composition low dose group, presses 0.2g/ chicken/sky injection; The D group is the pharmaceutical composition high dose group, presses 0.4g/ chicken/sky injection; The E group is fed by 0.3g/ chicken/sky spice for astragalin injection group (Dalian three instrument company limiteies).The result shows, adopts 'Shuang Hualian ' injection of the present invention with microsphere treatment chicken bursal disease viral disease tangible curative effect to be arranged.
Existing drug study result is as follows in medicine injectable microsphere contrast of the present invention:
| Numbering | Group | Mortality rate % | Cure rate % | Effective percentage % |
| A | Healthy group | 0 | 100.0(40/40) | 100.0(40/40) |
| B | Negative control group | 62.5(25/40) | 5.0(2/40) | 32.5.0(13/40) |
| C | Low dose therapy group of the present invention | 15.0(6/40) | 50.0(20/40) | 80.0(32/40) |
| D | High-dose therapy group of the present invention | 7.5(3/40) | 75.0(30/40) | 87.5(35/40) |
| E | " astragalin injection " treatment group | 20.0(8/40) | 37.5(15/40) | 67.5(27/40) |
This result of the test shows, pharmaceutical composition is low, high-dose therapy group and matched group difference highly significant (P＜0.01), illustrates that pharmaceutical composition of the present invention has significant therapeutical effect to the chicken bursal disease viral disease that virus causes; Particularly high dose is used and obviously is better than " astragalin injection " treatment group curative effect (P＜0.01).
Above-mentioned detailed description of the 'Shuang Hualian ' injection for the treatment of avian viral diseases being carried out with microsphere and preparation method thereof with reference to embodiment; be illustrative rather than determinate; can list several embodiment according to institute's limited range; therefore in the variation and the modification that do not break away under the general plotting of the present invention, should belong within protection scope of the present invention.
Claims (6)
1, a kind of 'Shuang Hualian ' injection microsphere for the treatment of avian viral diseases, it is characterized in that comprising that 2～60% the extract by Chinese crude drug is that 39～95% molecular weight ranges of active component and microsphere weight is 4,000～9000, biodegradable medicinal high polymer adjuvant between 000 is formed, the composition of its Chinese crude drug % proportioning by weight is: Rhizoma Coptidis 13%～40%, Flos Lonicerae 15%～45%, Fructus Forsythiae 25%～61%, the Radix Astragali 11%～40%.
2, the 'Shuang Hualian ' injection microsphere of treatment avian viral diseases according to claim 1 is characterized in that the preferred weight percentage composition of described active component crude drug is: Rhizoma Coptidis 24.7%, Flos Lonicerae 26.7%, Fructus Forsythiae 26.3%, the Radix Astragali 22.3%.
3, the 'Shuang Hualian ' injection microsphere of treatment avian viral diseases according to claim 1 is characterized in that described medicinal high polymer adjuvant is selected from polylactide-co-glycolide, polylactic acid, polyglycolic acid, poly--the 3-butyric ester, polylactic acid-polyglycolic acid, polylactic acid. glycolic, poly-adjacent ester, polylactone, polyanhydride, poly butyric ester-hydroxyl pentanoate copolymer, polyglycolic acid, polypropylene glucosan, hydroxyacetic acid, polylactic acid-polyglycol, polyglycolic acid. and Polyethylene Glycol one or more mixing wherein.
4, the 'Shuang Hualian ' injection microsphere of treatment avian viral diseases according to claim 1 is characterized in that excellent polylactide-co-glycolide, polylactic acid, the polylactic acid of being selected from of described medicinal high polymer adjuvant. one or more mixing wherein of polyglycolic acid, polylactic acid-glycollic acid, poly butyric ester-hydroxyl pentanoate copolymer.
5, the 'Shuang Hualian ' injection microsphere of treatment avian viral diseases according to claim 1 is characterized in that described medicinal high polymer adjuvant is a polylactide-co-glycolide; Its molecular weight of described polylactide-co-glycolide is between 9000～13000 dalton; Lactide and Acetic acid, hydroxy-, bimol. cyclic ester polymerization ratio are between 40: 60～60: 40 in the polylactide-co-glycolide.
6, a kind of 'Shuang Hualian ' injection of treatment avian viral diseases according to claim 1 preparation method of microsphere, promptly adopt the emulsifying dispersion method to make, it is characterized in that implementation step comprises: use dichloromethane, chloroform, ethyl acetate, dioxane, ether, acetone, oxolane, glacial acetic acid and the mixed acid be made up of them are medicinal substances extract and the dissolving of biodegradable pharmaceutic adjuvant, wherein the bulking value percent of pharmaceutic adjuvant in organic solvent is 1～30%, the emulsifying agent that organic facies adopts is the nonionic emulsifier of HLB=4.5～6.0, use polyvinyl alcohol, polyvinyl pyrrolidone, consumption are 0.01～12% of organic facies; Sodium polymethacrylate, sodium polyacrylate, sodium carboxymethyl cellulose solution in addition, prepare continuous water, wherein they are 0.01～10.0 in the percetage by weight of aqueous phase, the emulsifying agent of aqueous phase is the nonionic emulsifier of HLB=14.0～15.5, consumption is 0.01～12% of a water, and the volume ratio of organic facies and water is 1: 4～100; Decentralized photo slowly is injected in the continuous phase by tubule, and emulsifying is sieved then, is drying to obtain.
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| CN102225103A (en) * | 2011-05-30 | 2011-10-26 | 河南黑马动物药业有限公司 | Formulation and production process of traditional Chinese medicinal injection solution for controlling Newcastle disease |
| CN102949462A (en) * | 2011-08-30 | 2013-03-06 | 河南金尔康动物药业有限公司 | Veterinary Shuanghuanglian preparation and preparation method for same |
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| CN102225103A (en) * | 2011-05-30 | 2011-10-26 | 河南黑马动物药业有限公司 | Formulation and production process of traditional Chinese medicinal injection solution for controlling Newcastle disease |
| CN102949462A (en) * | 2011-08-30 | 2013-03-06 | 河南金尔康动物药业有限公司 | Veterinary Shuanghuanglian preparation and preparation method for same |
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| CN102988490A (en) * | 2012-11-26 | 2013-03-27 | 河南新正好生物工程有限公司 | Application of Shuanghuanglian oral liquor in preparation of medicine for treating and preventing poultry virosis |
| CN103463225A (en) * | 2013-09-11 | 2013-12-25 | 丁玉兰 | Traditional Chinese medicine micro-ecologic preparation for preventing and treating newcastle disease and preparation method thereof |
| CN113200849A (en) * | 2021-04-27 | 2021-08-03 | 香港理工大学 | Separation and purification method of synthesized 3-hydroxybutyrate oligomer or oligomer and application of synthesized 3-hydroxybutyrate oligomer or oligomer in antibacterial and antiviral products |
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