CN101341252B - 啮齿动物细胞中的蛋白质表达 - Google Patents

啮齿动物细胞中的蛋白质表达 Download PDF

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Publication number
CN101341252B
CN101341252B CN200680040367XA CN200680040367A CN101341252B CN 101341252 B CN101341252 B CN 101341252B CN 200680040367X A CN200680040367X A CN 200680040367XA CN 200680040367 A CN200680040367 A CN 200680040367A CN 101341252 B CN101341252 B CN 101341252B
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China
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chinese hamster
hamster ovary
heterologous polypeptide
ovary celi
plasmid
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Expired - Fee Related
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CN200680040367XA
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Chinese (zh)
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CN101341252A (zh
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U·格普费特
E·科佩特茨基
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F Hoffmann La Roche AG
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F Hoffmann La Roche AG
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/16011—Herpesviridae
    • C12N2710/16211—Lymphocryptovirus, e.g. human herpesvirus 4, Epstein-Barr Virus
    • C12N2710/16251—Methods of production or purification of viral material
    • C12N2710/16252—Methods of production or purification of viral material relating to complementing cells and packaging systems for producing virus or viral particles
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/10—Plasmid DNA
    • C12N2800/108—Plasmid DNA episomal vectors

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
CN200680040367XA 2005-10-28 2006-10-26 啮齿动物细胞中的蛋白质表达 Expired - Fee Related CN101341252B (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP05023611 2005-10-28
EP05023611.6 2005-10-28
PCT/EP2006/010308 WO2007048601A1 (en) 2005-10-28 2006-10-26 Protein expression in rodent cells

Publications (2)

Publication Number Publication Date
CN101341252A CN101341252A (zh) 2009-01-07
CN101341252B true CN101341252B (zh) 2011-08-17

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CN200680040367XA Expired - Fee Related CN101341252B (zh) 2005-10-28 2006-10-26 啮齿动物细胞中的蛋白质表达

Country Status (7)

Country Link
US (1) US7892790B2 (https=)
EP (1) EP1945781B1 (https=)
JP (2) JP5666776B2 (https=)
CN (1) CN101341252B (https=)
CA (1) CA2624685C (https=)
ES (1) ES2461591T3 (https=)
WO (1) WO2007048601A1 (https=)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8530233B2 (en) * 2007-10-02 2013-09-10 Genetai Inc Expression vector with enhanced gene expression capacity and method for using the same
CA2722835C (en) 2008-05-15 2018-11-27 National Research Council Of Canada Process, vectors and engineered cell lines for enhanced large-scale transfection
US8822214B2 (en) * 2009-09-15 2014-09-02 Medimmune Limited Cells for transient expression and uses thereof
CN102453729B (zh) * 2010-10-29 2016-03-02 宁波安柯生物技术有限公司 一种快速高通量生产重组蛋白的平台
WO2017032834A1 (en) * 2015-08-25 2017-03-02 Lonza Biologics Plc. Mammalian expression system
US10464840B2 (en) * 2016-10-05 2019-11-05 Corning Incorporated Near infrared shielding and laser-resistant window
CN114174540A (zh) * 2019-07-25 2022-03-11 豪夫迈·罗氏有限公司 用于检测爱泼斯坦巴尔病毒(ebv)的组合物和方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4686186A (en) 1984-09-26 1987-08-11 Wisconsin Alumni Research Foundation Recombinant vector and eukaryotic host transformed thereby
IE913929A1 (en) 1990-11-13 1992-05-20 Immunex Corp Bifunctional selectable fusion genes
AU6953394A (en) 1993-05-21 1994-12-20 Targeted Genetics Corporation Bifunctional selectable fusion genes based on the cytosine deaminase (cd) gene
EP0760861A1 (en) 1994-05-20 1997-03-12 The Board Of Trustees Of The Leland Stanford Junior University An autonomous replication system for mammalian cells
US6479279B2 (en) 1995-12-29 2002-11-12 Estonian Biocentre Episomal vectors and uses thereof
US5888809A (en) 1997-05-01 1999-03-30 Icos Corporation Hamster EF-1α transcriptional regulatory DNA
US5976807A (en) 1998-03-18 1999-11-02 Pharmacopeia, Inc. Eukaryotic cells stably expressing genes from multiple transfected episomes
MY139948A (en) 2000-09-28 2009-11-30 Bayer Corp Enhanced transfection system
AU2002257440A1 (en) 2001-05-07 2002-11-18 National Research Council Of Canada Enhanced production of recombinant proteins by transient transfection of suspension-growing mammalian cells
WO2004018506A2 (en) 2002-08-22 2004-03-04 Cytos Biotechnology Ag Inducible alphaviral/orip based gene expression system
AU2003283174A1 (en) 2002-12-11 2004-06-30 Cytos Biotechnology Ag Method for protein production
AR046071A1 (es) 2003-07-10 2005-11-23 Hoffmann La Roche Anticuerpos contra el receptor i del factor de crecimiento de tipo insulinico y los usos de los mismos
US7919270B2 (en) * 2003-09-09 2011-04-05 Acyte Biotech Pty Ltd Mammalian expression system

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
H Mizuguchi等.Long-term replication of Epstein-Barr virus-derived episomal vectors in the rodent cells.《FEBS Letters》.2000,第472卷173-178. *
K-I Tomiyasu等.Gene transfer in vitro and in vivo with Epstein-Barr virus-based episomal vector results in markedly high transient expression in rodent cells.《Biochemical and biophysical research communications》.1998,第253卷(第3期),733-738. *

Also Published As

Publication number Publication date
WO2007048601A1 (en) 2007-05-03
EP1945781B1 (en) 2014-03-05
US7892790B2 (en) 2011-02-22
US20100021965A1 (en) 2010-01-28
JP5666776B2 (ja) 2015-02-12
CA2624685A1 (en) 2007-05-03
ES2461591T3 (es) 2014-05-20
JP2009513117A (ja) 2009-04-02
CN101341252A (zh) 2009-01-07
CA2624685C (en) 2014-06-03
EP1945781A1 (en) 2008-07-23
JP2012090633A (ja) 2012-05-17

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Granted publication date: 20110817

Termination date: 20211026