CN101336955B - Gynostemma pentaphylla extract and preparation method thereof and use thereof in preparing medicine for preventing and treating fatty liver and sub-health - Google Patents
Gynostemma pentaphylla extract and preparation method thereof and use thereof in preparing medicine for preventing and treating fatty liver and sub-health Download PDFInfo
- Publication number
- CN101336955B CN101336955B CN2008101457108A CN200810145710A CN101336955B CN 101336955 B CN101336955 B CN 101336955B CN 2008101457108 A CN2008101457108 A CN 2008101457108A CN 200810145710 A CN200810145710 A CN 200810145710A CN 101336955 B CN101336955 B CN 101336955B
- Authority
- CN
- China
- Prior art keywords
- herb gynostemmae
- gynostemmae pentaphylli
- group
- total glycosides
- extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention discloses a Gynostemma pentaphyllum extract containing Gynostemma pentaphyllum polysaccharides and Gynostemma pentaphyllum total saponins at a weight ratio of 1:(1-5). The invention also discloses an application thereof in preparing medicaments for preventing and treating fatty liver and sub-health, and a preparation method thereof. The Gynostemma pentaphyllum extract has good effects of preventing and treating fatty liver and sub-health.
Description
Technical field
The present invention relates to the extract of Herb Gynostemmae Pentaphylli, belong to field of medicaments.
Background technology
The main pharmacodynamics composition of Herb Gynostemmae Pentaphylli is a Herb Gynostemmae Pentaphylli total glycosides, has the activity similar to the ginsenoside, and does not have toxicity, and character is steady.Herb Gynostemmae Pentaphylli by its blood fat reducing, immunomodulating and antioxidation, is improved Abwehrkraft des Koepers as edible Chinese medicine health care product, reaches its resisting fatigue and anti-aging effects then.
Herb Gynostemmae Pentaphylli total glycosides can improve the oxidation resistance of body, effectively suppresses lipid peroxidation product and forms; Dense, the sticking state of the blood that can improve hyperlipidemia rats.Can significantly improve immune organ thymus, spleen weight, improve obviously superoxide dismutase activity improving of immune function of mice and energy, suppress malonaldehyde and generate, NOS, NO content also obviously reduce.
Herb Gynostemmae Pentaphylli polysaccharides has significant cellular immunization facilitation, and has effects such as blood sugar lowering, antioxidation, defying age, antitumor, Cure for insomnia.
Gypenoside has ginsenoside's physiologically active, and the Herb Gynostemmae Pentaphylli polysaccharides composition also is one of effective ingredient of Herb Gynostemmae Pentaphylli, therefore can consider comprehensive utilization when the exploitation Herb Gynostemmae Pentaphylli.Though and nearly 20 years a lot of to the research of Herb Gynostemmae Pentaphylli, effectively the research of proportioning is used and is not seen and publish between Herb Gynostemmae Pentaphylli total glycosides and Herb Gynostemmae Pentaphylli polysaccharides.
Summary of the invention
First purpose of the present invention is to provide a kind of Herb Gynostemmae Pentaphylli extract, is formed by the Herb Gynostemmae Pentaphylli total glycosides and the Herb Gynostemmae Pentaphylli polysaccharides proportioning of special ratios, can be used for prevention and treatment fatty liver and sub-health state.
The present invention adopts following technical scheme: a kind of Herb Gynostemmae Pentaphylli extract, contain Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides, and the weight ratio of Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides is 1: 1-5.
The present invention also provides a kind of new medical usage of Herb Gynostemmae Pentaphylli extract, the application of Herb Gynostemmae Pentaphylli extract in prevention and treatment fatty liver medicine, and described Herb Gynostemmae Pentaphylli extract is 1 by Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides by weight: the ratio of 1-5 is formed.
The application of Herb Gynostemmae Pentaphylli extract in prevention and treatment sub-health state medicine, described Herb Gynostemmae Pentaphylli extract is 1 by Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides by weight: the ratio of 1-5 is formed.
The present invention also provides the preparation method of above-mentioned Herb Gynostemmae Pentaphylli extract, comprises following process:
(1) get the clean medical material extracting in water of Herb Gynostemmae Pentaphylli, and to regulate pH with the calcium carbonate fine powder is 8-10, after the filtration extracting solution carried out vacuum concentration;
(2) add edible ethanol in concentrated solution, the limit edged is stirred to and contains alcohol amount and reach 59～61% by volume, leaves standstill, and gets supernatant; Precipitate washs with edible ethanol, collects cleaning mixture and supernatant and merges standby;
(3) after supernatant is put and is reclaimed ethanol in the alcohol distillation column, by macroporous resin column D101, with volumetric concentration be 30%～70% edible ethanol be washed till eluent colourless after, collect eluent decompression recycling ethanol and concentrated, the relative density that concentrates back extractum is 1.20～1.25, with getting yellow powder behind the extract dry, be Herb Gynostemmae Pentaphylli total glycosides;
(4) precipitate after the washing volatilizes ethanol in the step (2), uses the distilled water reflux, extract,, gets the centrifugal precipitation of removing of supernatant, add ethanol behind the supernatant concentrating under reduced pressure, make to contain alcohol amount and reach 79～81% by volume, leave standstill, centrifugal, the precipitate drying gets Herb Gynostemmae Pentaphylli polysaccharides;
(5) Herb Gynostemmae Pentaphylli polysaccharides that extraction is obtained and Herb Gynostemmae Pentaphylli total glycosides are 1 by weight: the ratio proportioning of 1-5.
Studies show that the effect that Herb Gynostemmae Pentaphylli has invigorating the spleen and benefiting QI, heat-clearing and toxic substances removing, braces up energy and delay senility, have two-way immunomodulating, resist myocardial ischemia and improve multiple physiologically actives such as cardiac function, slow down aging, antitumor.Herb Gynostemmae Pentaphylli has been improved Abwehrkraft des Koepers by above physiologically active, brings into play its resisting fatigue and anti-aging effects then, is used to prevent and treat sub-health state based on chronic fatigue syndrome, fatty liver etc.Show by test, its mechanism of action of Herb Gynostemmae Pentaphylli extract of the present invention can be organized free-radical contents by removing, improve the oxidation resistance of body, reduce hepatic tissue TG, TC, FFA content, improve that the athero of liver realizes, have the effect of preventing and treating fatty liver and sub-health state preferably.
Description of drawings
Fig. 1 is blank group hepatic tissue pathology section HE dyeing 400 * microphotograph,
Fig. 2 is model group hepatic tissue pathology section HE dyeing 400 * microphotograph,
Fig. 3 is lovastatin group hepatic tissue pathology section HE dyeing 400 * microphotograph,
Fig. 4 is Herb Gynostemmae Pentaphylli total glycosides group hepatic tissue pathology section HE dyeing 400 * microphotograph,
Fig. 5 is the new B component group of Herb Gynostemmae Pentaphylli hepatic tissue pathology section HE dyeing 400 * microphotograph,
Fig. 6 is the new component A group of Herb Gynostemmae Pentaphylli hepatic tissue pathology section HE dyeing 400 * microphotograph.
The specific embodiment
The invention will be further described below in conjunction with specific embodiment.
Embodiment 1
Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides are to adopt following step to extract among the embodiment 2-6:
(1) getting the clean medical material of Herb Gynostemmae Pentaphylli drops in the ultrasound wave Chinese medicine extraction jar, add 0.5% calcium carbonate fine powder to regulate pH=8-10, extracting in water 2 times, filter the back and in two effect concentration evaporators, carry out vacuum concentration, the vacuum concentration effective temperature is controlled at 80～85 ℃, vacuum degree control is at-0.06～0.07MPa, and the simmer down to relative density is 1.15～1.20 (80 ℃ of heat are surveyed), cools.
(2) concentrated solution adds 95% (v/v) edible ethanol, and the limit edged is stirred to and contains the alcohol amount and reach 59～61% (v/v), leaves standstill 12 hours, gets supernatant; Precipitate is collected cleaning mixture and supernatant and is merged standby with 60% (v/v) edible ethanol washing 2 times.
(3) after supernatant is put and is reclaimed ethanol in the alcohol distillation column, by macroporous resin column D101, with 40%～60% (v/v) edible ethanol be washed till eluent colourless after, collect eluent decompression recycling ethanol and concentrated, the relative density that concentrates back extractum is 1.20～1.25, yellow powder (Herb Gynostemmae Pentaphylli total glycosides, standby) will be got behind the extract dry.
(4) precipitate after the washing in the step (2) is volatilized ethanol, with distilled water reflux, extract, 2 times, each 10 minutes, merge supernatant, the centrifugal precipitation of removing, the supernatant concentrating under reduced pressure adds 95% (v/v) ethanol, makes to contain the alcohol amount and reach 79～81% (v/v), low temperature spends the night, centrifugal, the precipitate cold drying gets Herb Gynostemmae Pentaphylli polysaccharides.
(5) Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides are prepared in proportion.
Embodiment 2 Herb Gynostemmae Pentaphylli total glycosides sheets
Prescription: Herb Gynostemmae Pentaphylli total glycosides 20g starch 180
Get the method mixing that above-mentioned raw materials adopts equivalent to increase progressively in blender, full pressed powder, every heavy 200mg contains Herb Gynostemmae Pentaphylli total glycosides 20mg.
The new component A of embodiment 3. Herb Gynostemmae Pentaphylli
Prescription: Herb Gynostemmae Pentaphylli total glycosides 20g Herb Gynostemmae Pentaphylli polysaccharides 5g starch 175g
Get the method mixing that above-mentioned raw materials Herb Gynostemmae Pentaphylli total glycosides and Herb Gynostemmae Pentaphylli polysaccharides adopt equivalent to increase progressively in blender, full pressed powder, every 200mg, every contains Herb Gynostemmae Pentaphylli total glycosides 20mg, Herb Gynostemmae Pentaphylli polysaccharides 5mg.
The new component A1 of embodiment 4. Herb Gynostemmae Pentaphylli
Prescription: Herb Gynostemmae Pentaphylli total glycosides 20g Herb Gynostemmae Pentaphylli polysaccharides 20g starch 160g
Get the method mixing that above-mentioned raw materials gynostemma pentaphyllum total soap and Herb Gynostemmae Pentaphylli polysaccharides adopt equivalent to increase progressively in blender, full pressed powder, every 200mg, every contains Herb Gynostemmae Pentaphylli total glycosides 20mg, Herb Gynostemmae Pentaphylli polysaccharides 20mg.
The new B component of embodiment 5. Herb Gynostemmae Pentaphylli
Prescription: Herb Gynostemmae Pentaphylli total glycosides 20g Herb Gynostemmae Pentaphylli polysaccharides 3g starch 160g
Get the method mixing that above-mentioned raw materials Herb Gynostemmae Pentaphylli total glycosides and Herb Gynostemmae Pentaphylli polysaccharides adopt equivalent to increase progressively in blender, full pressed powder, every 200mg, every contains Herb Gynostemmae Pentaphylli total glycosides 20mg, Herb Gynostemmae Pentaphylli polysaccharides 3mg.
The new component C of embodiment 6. Herb Gynostemmae Pentaphylli
Prescription: Herb Gynostemmae Pentaphylli total glycosides 20g Herb Gynostemmae Pentaphylli polysaccharides 25g starch 155g
Get the method mixing that above-mentioned raw materials Herb Gynostemmae Pentaphylli total glycosides and Herb Gynostemmae Pentaphylli polysaccharides adopt equivalent to increase progressively in blender, full pressed powder, every 200mg, every contains Herb Gynostemmae Pentaphylli total glycosides 20mg, Herb Gynostemmae Pentaphylli polysaccharides 25mg.
Below by the elaboration of the new component of Herb Gynostemmae Pentaphylli being carried out animal experiment study and clinical research, the effect of the present invention aspect treatment fatty liver, fat blood fat reducing is described, and confirmed that the new component of Herb Gynostemmae Pentaphylli is to being the preventive and therapeutic effect of the sub-health state of cardinal symptom with the confirmed fatigue.
(1) Herb Gynostemmae Pentaphylli extract is to the experimentation of metabolic disease influence
1. the influence of antioxidation
1.1 method: 40 of rats, male, body weight 240g ± 12g is divided into 4 groups at random:
The A group: normal group, give distilled water;
The B group: model group, give CCl
4(150mg/kg);
C group: CCl
4Add Herb Gynostemmae Pentaphylli total glycosides sheet (15mg/kg);
D group: CCl
4Add the new component A of Herb Gynostemmae Pentaphylli (15mg/kg).
Herb Gynostemmae Pentaphylli and distilled water group be gastric infusion (1ml/100g) once a day, irritates the preceding fasting 16h of stomach, continuously 10d.CCl
4In the 1st, the 3d intraperitoneal injection.24h puts to death animal after the last administration, taking out liver immediately puts into the floating blood of 4 ℃ of normal saline flush awaies, divests fat and connective tissue, wipe dry, weigh, shred, press the 1g tissue and add 9ml 0.25mol/Lsucrose-0.01mol/L Tris-HCl buffer, homogenate.Get that the part liver homogenate is centrifugal through 500g, after 20min removes the karyon fragment, through the centrifugal 10min of 104g, precipitate is a mitochondrion.Get supernatant through the centrifugal 65min of 105g, precipitate is a microsome.Liver homogenate, microsome, mitochondrial lipid peroxidation thing (LPO) content TBA colorimetric method for determining; Glutathion peroxidase (GSH-Px) activity dtnb assay.With enzymatic assays serum glutamic pyruvic transminase (ALT).Protein content is measured with the Lowry method.Add up with the one factor analysis of variance method.
1.2 result:
1.2.1 the Herb Gynostemmae Pentaphylli active component significantly is lower than the B group to the active C of influence of rat ALT, D group ALT, but still is higher than the A group, sees Table 1, shows that Herb Gynostemmae Pentaphylli extract can weaken CCl
4To the infringement of liver function, and the new component A of Herb Gynostemmae Pentaphylli that organizes with D is excellent (P＜0.01).
1.2.2 the new component A of Herb Gynostemmae Pentaphylli is to the active influence of rat liver LPO, GSH-Px
C, D group LPO is starkly lower than B group, and the GSH-Px activity is higher than the B group, though C, D group LPO and GSH-Px do not return to A group level fully, compares with the B group, significant difference (P＜0.05), and be excellent with the new component A of Herb Gynostemmae Pentaphylli.See Table 2.
The table 1 pair active influence of rat ALT (X ± S, n=10)
Annotate: compare * P＜0.05, * * P＜0.01 with model group;
The table 2 pair active influence of rat liver LPO, GSH-Px (X ± S, n=10)
Annotate: compare * P＜0.05, * * P＜0.01 with model group
Conclusion: Herb Gynostemmae Pentaphylli total glycosides and Herb Gynostemmae Pentaphylli polysaccharides can improve the oxidation resistance of body by the compositions that special ratios of the present invention forms, and can suppress lipid peroxidation product formation; And the free radical of the impaired rat of energy the liver protecting is dezymotized the vigor that is, weakens the lipid peroxidation that free radical acts on liver plasma membrane, thereby has weakened CCl to a certain extent
4To the damage of liver, and be better than simple Herb Gynostemmae Pentaphylli total glycosides.
The influence of the 2 pairs of obese rat body weight, fat content in abdominal cavity, serum total cholesterol, triglyceride
2.1 method: select 56 of body weight 150～180g healthy rats for use, male and female half and half are divided into 7 groups at random:
A group: normal group
B group: model group
C group: Herb Gynostemmae Pentaphylli total glycosides sheet group
D group: the new component A group of Herb Gynostemmae Pentaphylli
E group: the new component B group of Herb Gynostemmae Pentaphylli
F group: the new component C group of Herb Gynostemmae Pentaphylli
G group: the new component A1 group of Herb Gynostemmae Pentaphylli
A group (normal group) is fed normal diet, and B group (model group), C-G group Herb Gynostemmae Pentaphylli extract group are all fed high lipid food (including 2% cholesterol, 10% Adeps Sus domestica, 0.5% sodium cholate, 4% white sugar, 83.5% normal feedstuff).A group and B organize every morning and press 2ml/100g to tap water, C-F group gastric infusion 0.03g/kg, 2ml/100g, continuous 40 days, cut the tail blood sampling after empty stomach was weighed in the 41st day and survey blood fat, continue administration and raise to the 44th day, take a blood sample with pentobarbital sodium (40mg/kg) intraperitoneal injection of anesthesia rear neck artery, anticoagulant heparin is surveyed hemorheology, and cuts open and get liver observation hepatic steatosis situation, measures fat content in abdominal cavity.
2.2 result:
2.2.1 influence and B group (model group) to the obese rat blood fat compare, C group (Herb Gynostemmae Pentaphylli total glycosides group), D group (the new component A group of Herb Gynostemmae Pentaphylli), G group (the new component A1 group of Herb Gynostemmae Pentaphylli) TC, TG level descend obviously, there were significant differences, HDL-c there is rising trend, LDL-c, AI significantly descend, and effect for reducing blood fat is with D group (the new component A group of Herb Gynostemmae Pentaphylli) effect more excellent (P＜0.01).E group (the new B component of Herb Gynostemmae Pentaphylli) is organized, part index number also has effect to F group (the new component C group of Herb Gynostemmae Pentaphylli), but effect is relatively poor, sees Table 3.
2.2.2 influence and B group (model group) to obese rat hemorheology, body weight increment, liver fatty liver incidence rate and fat content in abdominal cavity compare, C group (Herb Gynostemmae Pentaphylli total glycosides group), D group (the new component A group of Herb Gynostemmae Pentaphylli), G group (the new component A1 group of Herb Gynostemmae Pentaphylli) whole blood contrast viscosity, packed cell volume, body weight increment and fat content in abdominal cavity obviously reduce, B group fat accounts for 4.26% of its body weight, C group, D group, G organize 4.1% and 3.79% of abdominal cavity fat percentage of liveweight, 3.87%, E group and F group are respectively 4.2% and 4.15%, effect is relatively poor, sees Table 4.
The influence of table 3 pair obese rat blood fat (X ± S, n=8)
Annotate: compare * P＜0.05, * * P＜0.01 with model group.
Table 4 pair obese rat hemorheology, body weight increment, liver fatty liver incidence rate and abdominal cavity fat
The influence of content (X ± S, n=8)
Annotate: compare * P＜0.05, * * P＜0.01 with model group.
Conclusion: Herb Gynostemmae Pentaphylli total glycosides and dense, the sticking state of blood that all can improve hyperlipidemia rats by the compositions that Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides are formed, slow down body weight, suppress the generation of fatty liver, has the minimizing lipidosis, slimming effect is with the new component A of Herb Gynostemmae Pentaphylli, the new component A1 good effect of Herb Gynostemmae Pentaphylli.
(2) Herb Gynostemmae Pentaphylli total glycosides and compositions are studied the effect of mixed type Prevention and Management of Fatty Liver
1.1. laboratory animal
104 of healthy SPF level SD male rats (body weight 170 ± 20g) is provided by Guangdong Province's Experimental Animal Center, animal quality certification SCXK (Guangdong) 2003-0002, and word 2007A003 checks and affirm in Guangdong.
1.2. medicine
Herb Gynostemmae Pentaphylli total glycosides sheet (embodiment 2), the new component A of Herb Gynostemmae Pentaphylli (embodiment 3), the new B component of Herb Gynostemmae Pentaphylli (embodiment 5), the new component C of Herb Gynostemmae Pentaphylli (embodiment 6), the preparation of the new component A1 of Herb Gynostemmae Pentaphylli (embodiment 4) Guangzhou Baiyunshan Heji Huangpu Chinese Medicine Co., Ltd..
Lovastatin tablet (lot number: 20060901) BeiJing WanSheng Pharmacy Co., Ltd.
Edible ethanol (Red Star strong, colourless liquor distilled from sorghum, lot number: GB/T10781.2-89, alcoholic strength 55%VOL) Hongxing Co., Ltd. Beijing's (hereinafter to be referred as ethanol).
1.3. instrument
Its woods Bel instrument Manufacturing Co., Ltd of Haimen City, vortex mixer Jiangsu
LAC-110.4 type electronic analytical balance Germany Sai Duolisi (ACCULAB) joint-stock company
TDL80-2B centrifuge Anting Scientific Instrument Factory, Shanghai
TGL-16G type High speed refrigerated centrifuge Anting Scientific Instrument Factory, Shanghai
Cut Potter-Elvehjem Tissue Grinders West Zhejiang Province machinery plant in the ZS-831 type
The grand experimental facilities company limited of Nereid on the DK-S22 type electric-heated thermostatic water bath
Refrigerated centrifuge Sigma company
The general all purpose instrument company limited of analysing in TU-1800 ultraviolet-visible spectrophotometer Beijing
1.4. experimental technique
The rat adaptability was raised for 1 week, is divided into 10 groups at random:
A group: blank group;
B group: model group;
C group: lovastatin group;
D1 group: Herb Gynostemmae Pentaphylli total glycosides sheet low dose group;
D2 group: Herb Gynostemmae Pentaphylli total glycosides sheet high dose group;
E1 group: the new component A low dose group of Herb Gynostemmae Pentaphylli;
E2 group: the new component A high dose group of Herb Gynostemmae Pentaphylli;
F group: the new B component high dose group of Herb Gynostemmae Pentaphylli;
G group: the new component C high dose group of Herb Gynostemmae Pentaphylli;
H group: the new component A1 high dose group of Herb Gynostemmae Pentaphylli.
The positive medicine group of lovastatin group of C group wherein.
Every day, the observed and recorded rat situation of ingesting (was pressed 50g/kgd
-1The corresponding feedstuff an of/feed), weigh in the end weekly, changes corresponding adjustment administration and irritate 55 ° of Red Star strong, colourless liquor distilled from sorghum of stomach (medicine: lovastatin group 2mg/kgd according to rat body weight
-1/ only, Herb Gynostemmae Pentaphylli total glycosides sheet group and the new component of each Herb Gynostemmae Pentaphylli are low, high dose group is respectively 15mg/kgd
-1/ only (be equivalent to be grown up clinical consumption 5 times), 30mg/kgd
-1/ only (be equivalent to be grown up clinical consumption 10 times).Ethanol: 10ml/kgd
-1/ only).Every morning, blank group gave normal diet, model group and the new component group of each Herb Gynostemmae Pentaphylli give high lipid food (81% normal feedstuff+10% Adeps Sus domestica+0.5% sodium cholate+1.5% cholesterol+2% yolk powder+5% sugar, provide by Guangdong Province's Experimental Animal Center), model group and medicine group are irritated stomach ethanol simultaneously, and blank group waits the capacity normal saline.Every afternoon, the medicine group was irritated the medicine of stomach corresponding dosage, and blank group, model group give the equivalent normal saline.Observe the general situation (hair, diet, figure's activity, feces) of rat when irritate stomach every day.Slaughter animal and test detection accordingly the 10th weekend:
(1) observes food ration, body weight gain situation, calculating liver index.
(2) activity of the content of TC, TG, FFA, HDL-C, LDL-C, MDA and ALT, AST, SOD in the detection rat blood serum.
(3) detect content and ALF, the AST of TC, TG, FFA, MDA in the liver tissues of rats homogenate,, the activity of SOD.
(4) observing hepatic tissue pathology changes.
1.5 experimental result
1.5.1 general situation is observed
A group (blank group) activities in rats state, its hair is glossy, and the figure is active, and reaction is quick, and appetite, Excreta are all normal.There is drowsiness situation B group (model group) rat oral gavage ethanol rear section, and its hair is owed gloss, the movable minimizing behind the filling stomach ethanol, and it is more to sleep, rare soft during feces; The model group rat body weight of feed high lipid food increases blank relatively group performance obviously.
1.5.2 the new component of Herb Gynostemmae Pentaphylli total glycosides sheet and Herb Gynostemmae Pentaphylli is to fatty liver rat body weight and the exponential influence of liver
Table 5 is respectively organized rat body weight situation of change (χ ± s)
Table 5 continues rat body weight situation of change (χ ± s)
Annotate: compare with model group
*P＜0.05,
*P＜0.01.
Table 5 shows: each is organized rat body weight and increases very fast, wherein B group (model group) increases the fastest, when 7 weeks, begin apparently higher than the blank group, organize significance more statistically with blank during 8 weeks, with blank group the highly significant meaning is arranged more statistically when 9 weeks, 10 weeks, illustrate that the model group rat body weight of feed high lipid food increases blank relatively group performance obviously.And each medicine group body weight gain is slow than model group, there was no significant difference.
Table 6 Herb Gynostemmae Pentaphylli total glycosides and compositions thereof are to the exponential influence of rats'liver (χ ± s)
Annotate: compare with model group
*P＜0.05,
*P＜0.01
Table 6 shows: B group (model group) liver index has highly significant meaning (P＜0.01) apparently higher than A group (blank group) on the statistics.Low, the high dose group of the new component A group of Herb Gynostemmae Pentaphylli total glycosides and Herb Gynostemmae Pentaphylli, the new component A1 high dose group of Herb Gynostemmae Pentaphylli liver index is starkly lower than model group, with model group highly significant meaning (P＜0.01) is arranged more statistically, be lower than the lovastatin group, illustrate that the effect that Herb Gynostemmae Pentaphylli total glycosides and new component A organize, new component A1 group suppresses the liver hypertrophy is obvious.
1.5.3 gynostemma pentaphyllum total and new component are to the influence of fatty liver rat fat
Table 7 Herb Gynostemmae Pentaphylli total glycosides and compositions thereof are to the influence of rat blood serum TG, TC, FFA content (χ ± s)
Annotate: compare with model group
*P＜0.05,
*P＜0.01; Compare with the blank group
△P＜0.05,
△ △P＜0.01.
Table 7 shows: Herb Gynostemmae Pentaphylli total glycosides sheet and Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides compositions can reduce the content of rat blood serum TG, TC, FFA.
Table 8 Herb Gynostemmae Pentaphylli total glycosides and compositions thereof are to the influence of rat blood serum HDL-C, LDL-C content (χ ± s)
Annotate: compare with model group
*P＜0.05,
*P＜0.01; Compare with the blank group
△P＜0.05,
△ △P＜0.01.
Table 8 shows: Herb Gynostemmae Pentaphylli total glycosides and can reduce rat blood serum LDL-C content with compositions that Herb Gynostemmae Pentaphylli polysaccharides is formed, and rising HDL-C content, and composition effect is more obvious.
1.5.4. gynostemma pentaphyllum total and new component thereof are to the influence of fatty liver rats'liver fat
Table 9 Herb Gynostemmae Pentaphylli total glycosides and compositions thereof are to the influence of liver tissues of rats TG, TC, FFA content (χ ± s)
Annotate: compare with model group
*P＜0.05,
*P＜0.01; Compare with the blank group
△P＜0.05,
△ △P＜0.01.
Table 9 shows: Herb Gynostemmae Pentaphylli total glycosides and can reduce liver tissues of rats TG, TC, FFA content with compositions that Herb Gynostemmae Pentaphylli polysaccharides forms
1.5.5 Herb Gynostemmae Pentaphylli total glycosides and component thereof are to the influence of fatty liver rats'liver function
Table 10 Herb Gynostemmae Pentaphylli total glycosides and new component thereof are to the influence of rat blood serum and hepatic tissue ALT, AST content (χ ± s)
Annotate: compare with model group
*P＜0.05,
*P＜0.01; Compare with the blank group
△P＜0.05,
△ △P＜0.01.
Table 10 shows: Herb Gynostemmae Pentaphylli total glycosides and have the effect of the liver protecting and ALT lowering with the compositions that Herb Gynostemmae Pentaphylli polysaccharides constitutes, but curative effect with the new component A of Herb Gynostemmae Pentaphylli for well.
1.5.6 Herb Gynostemmae Pentaphylli total glycosides and combination thereof are cut into slices to mixed type fatty liver hepatic tissue pathology
Section is as Fig. 1-shown in Figure 6.As seen from Figure 1, Combination fatty liver rat model hepatic tissue HE dyeing pathology picture shows blank treated animal hepatocyte structural integrity, and nuclear is placed in the middle, the hepatic cords marshalling.As seen from Figure 2, the fat drop that the appearance of model group hepatocyte is differed in size widely, quantity differs, nucleus is pushed obvious off normal by fat drop, and fat becomes performance obviously (arrow indication among the figure).As seen from Figure 3, lovastatin group zones of different hepatocyte occurs differing in size on every side, quantity differs fat drop.By Fig. 4 and Fig. 5 as can be seen, Herb Gynostemmae Pentaphylli total glycosides group, the new B component group of Herb Gynostemmae Pentaphylli also have fat to drip, and the parts of fine karyon is pressed against a side, and the new component C group of Herb Gynostemmae Pentaphylli is consistent with Fig. 5.As seen from Figure 6, the new component A group of Herb Gynostemmae Pentaphylli fat becomes the lightest, and fat drips less, and nuclear is placed in the middle, and the hepatocyte structure is more complete.From the therapeutic effect of mixed type fatty liver is analyzed, the curative effect of Herb Gynostemmae Pentaphylli total glycosides is 45%, the curative effect of the new component A of Herb Gynostemmae Pentaphylli is 87%, the curative effect of the new component A1 of Herb Gynostemmae Pentaphylli is 72%, the curative effect of the new B component of Herb Gynostemmae Pentaphylli is 42%, and the curative effect of the new component C of Herb Gynostemmae Pentaphylli is 37%, and visible Herb Gynostemmae Pentaphylli total glycosides and the new component of Herb Gynostemmae Pentaphylli all have the treatment crop to the mixed type fatty liver, but the new component A of Herb Gynostemmae Pentaphylli, the curative effect of the new component A1 of Herb Gynostemmae Pentaphylli obviously is better than other each group (P＜0.01).
(3) to preventing and treating the experimentation of sub-health state
The influence of the 1 pair of mouse immune organ thymus, spleen weight
1.1 method: get body weight 18-20g healthy mice, male and female half and half, random packet.Except that the blank group, all the other respectively organize equal subcutaneous injection cyclophosphamide 40mg/kg, once a day, for three days on end, cause the immunologic hypofunction model, blank group subcutaneous injection isometric(al) normal saline.From modeling gastric infusion on the same day, blank group and model group gavage the isometric(al) normal saline, once a day, and continuous 8 days.24h puts to death animal after the last administration, weighs, and wins thymus and spleen is weighed, with thymus or SBR as thymus or index and spleen index.
1.2 result: Herb Gynostemmae Pentaphylli can significantly improve immune organ thymus, spleen weight, improves immune function of mice, and with new component A of Herb Gynostemmae Pentaphylli and A1 effect optimum (P＜0.01).See Table 11.
Table 11 Herb Gynostemmae Pentaphylli is to the influence of mouse immune organ thymus, spleen weight (X ± S)
Annotate: compare * P＜0.05, * * P＜0.01 with model group.
2 experimental study about anti-senility
2.1 method: experiment is with male rat 220～250g, by the body weight random packet.Except that normal group, all the other respectively organize subcutaneous injection D-galactose (125mg/kg/d, filtration sterilization), after the injection, and not administration of model group, the Herb Gynostemmae Pentaphylli group is given the 100mg/kg.d gastric infusion, and the normal saline group gavages 1mL/kg/d isometric(al) normal saline, continuously 40d.The sacrificed by decapitation rat is taken out the inboard substrate tissue of hypothalamus rapidly then, weighs to be placed in the homogenizer and grinds, and homogenate is the centrifugal 30min of 2800r/min in the time of 4 ℃, gets supernatant-20 ℃ preservation, and is stand-by.Detect explanation according to test kit and detect wherein SOD, MDA, NOS, NO content.
2.2 result: aging model group SOD is active obviously to be reduced, and the MDA level obviously increases, and NOS, NO content all obviously increase, and with the normal control group significant difference (P＜0.05) are arranged relatively.After using the Herb Gynostemmae Pentaphylli treatment, the SOD activity obviously increases, and the MDA level obviously reduces, and NOS, NO content also obviously reduce.More all there were significant differences (P＜0.05 or P＜0.01) with normal control group and aging model group.Illustrate that Herb Gynostemmae Pentaphylli has delaying senility function.Its mechanism may be that the oxidation resistance of raising body realizes by removing cerebral tissue free-radical contents.See Table 12.
Subacute old and feeble rat hypothalamus SOD due to the table 12 pair D-galactose, MDA, NOS, NO content influence (X ± S)
Annotate: compare * P＜0.05, * * P＜0.01 with normal group; Compare #P＜0.05, ##P＜0.01 with model group.
The experimentation of 3 anti-stress effects
(1) mice anoxia enduring experiment
1.1 method: select healthy mice, body weight 18-20g, male and female half and half.Random packet.Gastric infusion 1 time every day (matched group isometric(al) distilled water), successive administration 5 days, 1h after the last administration puts into 50ml syringe (1 mice) with animal, with sealing compound two ends is shut, and makes it air tight.Timing immediately is an index with the respiratory arrest, and the observation mice causes anoxia and the dead time.
1.2 Herb Gynostemmae Pentaphylli and BUZHONG YIQI WAN all can obviously prolong the mice anoxia enduring time-to-live (P＜0.05) as a result.See Table 13.
Table 13 Herb Gynostemmae Pentaphylli is to the influence of anoxia in mice time-to-live (X ± S)
Annotate: compare * P＜0.05 with matched group
(2) mice endurance experiment (mice swimming test)
2.1 method: select healthy mice, body weight 18-22g, male and female half and half.Random packet, gastric infusion every day once (matched group isometric(al) distilled water), every treated animal was at the heavy heavy burden thing of afterbody bundle one 2g in the 7th day, animal is put into glass jar swims, the depth of water is to 20cm, and water temperature remains on 20 ℃ ± 0.5 ℃, and 10s can not emerge when the mouse head submerged, be muscle power and exhaust that timing at once is the mice swimming time.
2.2 each group of Herb Gynostemmae Pentaphylli and BUZHONG YIQI WAN all can prolong mice swimming time (P＜0.05) as a result.See Table 14.
Table 14 Herb Gynostemmae Pentaphylli is to the influence of mice swimming time (X ± S)
Annotate: compare * P＜0.05 with matched group
In sum, the new component of Herb Gynostemmae Pentaphylli total glycosides and Herb Gynostemmae Pentaphylli, protect the liver, fall aspects such as enzyme in prophylactic treatment fatty liver, obesity, blood fat reducing better action is arranged, and to being that the sub-health state of cardinal symptom and lipotropic effect are better than conventional Herb Gynostemmae Pentaphylli of extracting with the confirmed fatigue, and be excellent with new component A of Herb Gynostemmae Pentaphylli and A1 curative effect.
Claims (5)
1. Herb Gynostemmae Pentaphylli extract, it is characterized in that: active component is made up of Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides, and the weight ratio of Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides is 1: 1-5.
2. a Herb Gynostemmae Pentaphylli extract is as the application of unique active component in preparation prevention and treatment fatty liver medicine, and described Herb Gynostemmae Pentaphylli extract is 1 by Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides by weight: the ratio of 1-5 is formed.
3. a Herb Gynostemmae Pentaphylli extract is as the application of unique active component in preparing the sub-health state medicine that prevention and treatment are cardinal symptom with the confirmed fatigue, and described Herb Gynostemmae Pentaphylli extract is 1 by Herb Gynostemmae Pentaphylli polysaccharides and Herb Gynostemmae Pentaphylli total glycosides by weight: the ratio of 1-5 is formed.
4. the preparation method of a Herb Gynostemmae Pentaphylli extract comprises following process:
(1) get the clean medical material extracting in water of Herb Gynostemmae Pentaphylli, and to regulate pH with the calcium carbonate fine powder is 8-10, after the filtration extracting solution carried out vacuum concentration;
(2) add edible ethanol in concentrated solution, the limit edged is stirred to and contains alcohol amount and reach 59～61% by volume, leaves standstill, and gets supernatant; Precipitate washs with edible ethanol, collects cleaning mixture and supernatant and merges standby;
(3) with behind the reserve liquid recovery ethanol after step (2) cleaning mixture and the supernatant merging, by macroporous resin column D101, with volumetric concentration be 30%～70% edible ethanol be washed till eluent colourless after, collect eluent decompression recycling ethanol and concentrated, the relative density that concentrates back extractum is 1.20～1.25, with getting yellow powder behind the extract dry, be Herb Gynostemmae Pentaphylli total glycosides;
(4) precipitate after the washing volatilizes ethanol in the step (2), uses the distilled water reflux, extract,, gets the centrifugal precipitation of removing of supernatant, add ethanol behind the supernatant concentrating under reduced pressure, make to contain alcohol amount and reach 79～81% by volume, leave standstill, centrifugal, the precipitate drying gets Herb Gynostemmae Pentaphylli polysaccharides;
(5) Herb Gynostemmae Pentaphylli polysaccharides that extraction is obtained and Herb Gynostemmae Pentaphylli total glycosides are 1 by weight: the ratio proportioning of 1-5.
5. preparation method as claimed in claim 4 is characterized in that: the vacuum concentration effective temperature is 80～85 ℃ in the described step (1), vacuum is-0.06～-0.07MPa, the simmer down to relative density is 1.15～1.20 extractum.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101457108A CN101336955B (en) | 2008-08-11 | 2008-08-11 | Gynostemma pentaphylla extract and preparation method thereof and use thereof in preparing medicine for preventing and treating fatty liver and sub-health |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2008101457108A CN101336955B (en) | 2008-08-11 | 2008-08-11 | Gynostemma pentaphylla extract and preparation method thereof and use thereof in preparing medicine for preventing and treating fatty liver and sub-health |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101336955A CN101336955A (en) | 2009-01-07 |
| CN101336955B true CN101336955B (en) | 2011-11-16 |
Family
ID=40211220
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2008101457108A Active CN101336955B (en) | 2008-08-11 | 2008-08-11 | Gynostemma pentaphylla extract and preparation method thereof and use thereof in preparing medicine for preventing and treating fatty liver and sub-health |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101336955B (en) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103191172A (en) * | 2012-01-06 | 2013-07-10 | 上海家化联合股份有限公司 | Gynostemma pentaphylla extract, and preparation method and application thereof |
| CN111135212A (en) * | 2020-01-17 | 2020-05-12 | 安徽农业大学 | A black mulberry tea with liver protecting effect and its preparation method |
| CN113509477A (en) * | 2021-09-13 | 2021-10-19 | 徐世明 | Application and preparation of gypenoside B in antagonizing side effects of radiotherapy, chemotherapy and glucocorticoid medicine |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1098298A (en) * | 1993-08-05 | 1995-02-08 | 裴留成 | Herb Gynostemmae Pentaphylli total glycosides and polysaccharide formulation and preparation method |
-
2008
- 2008-08-11 CN CN2008101457108A patent/CN101336955B/en active Active
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1098298A (en) * | 1993-08-05 | 1995-02-08 | 裴留成 | Herb Gynostemmae Pentaphylli total glycosides and polysaccharide formulation and preparation method |
Non-Patent Citations (2)
| Title |
|---|
| JP昭60-43358A 1985.03.07 |
| 陈珏.植物药绞股兰在日本的研究概况.《浙江药学》.1986,第3卷(第4期),33-36. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101336955A (en) | 2009-01-07 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102273531B (en) | Radix hedysari and sealwort health preserving tea and health preserving capsule | |
| CN102771868A (en) | Dendrobium candidum solution | |
| CN102526478B (en) | Formula of health-care medicine with functions of strengthening immunity and reducing blood sugar | |
| CN107114787B (en) | Composition for preventing heat absorption | |
| CN106244371A (en) | A kind of blood sugar lowering Rhizoma Polygonati grape health care wine and production technology thereof | |
| CN101336955B (en) | Gynostemma pentaphylla extract and preparation method thereof and use thereof in preparing medicine for preventing and treating fatty liver and sub-health | |
| CN102258679A (en) | Traditional Chinese medicine composition and preparation method and application thereof | |
| CN103749818B (en) | A kind of sealwort hypoglycemic health protection tea and preparation method thereof | |
| CN106619782A (en) | Herba rhodiolae extract and healthy product containing herba rhodiolae extract | |
| CN104173451B (en) | The application in blood sugar lowering medicine and health food of a kind of natural drug composition | |
| CN1558768A (en) | A pharmaceutical composition made from Chinese traditional medicine and preparation method thereof | |
| CN100345566C (en) | Medicine composition used for reducing weight, prepn. method and use thereof | |
| CN101554406B (en) | Extraction process and formulation of mulberry cortex hypoglycemic medicinal active material | |
| CN101283761A (en) | Smallanthus sonchifolius fruit extract and extracting method and application | |
| CN110478398A (en) | A kind of pair of alcoholic liver injury has the Chinese medicine composition and preparation method of defencive function | |
| CN112704717A (en) | Stomach-invigorating medicinal and edible composition for assisting treatment of advanced digestive tract tumor | |
| CN105079532A (en) | Healthcare green tea capable of reducing blood fat and loosing weight and preparation method of healthcare green tea | |
| CN101336721A (en) | Health beverage and its preparation method | |
| CN109349477A (en) | A kind of dendrobium nobile compound drink and preparation method thereof | |
| CN104286263A (en) | Healthy longan herb tea drink and preparation method thereof | |
| CN103461600A (en) | Diet health-care weight-reducing tea granule | |
| CN108271964A (en) | A kind of Xuancheng's pawpaw beverage and its preparation method and application of shield stomach | |
| CN107441332A (en) | The ginseng composition and preparation method of auxiliary adjustment endocrine metabolism and anti-cancer and cancer-preventing | |
| CN106754057A (en) | A kind of health care red starter glutinous wine and preparation method thereof | |
| CN105169187B (en) | A kind of composition and its application, preparation method and drug, food containing the composition |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant |