CN101327168A - Method for extracting sea mud active component - Google Patents
Method for extracting sea mud active component Download PDFInfo
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- CN101327168A CN101327168A CNA200810063429XA CN200810063429A CN101327168A CN 101327168 A CN101327168 A CN 101327168A CN A200810063429X A CNA200810063429X A CN A200810063429XA CN 200810063429 A CN200810063429 A CN 200810063429A CN 101327168 A CN101327168 A CN 101327168A
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- 238000000034 method Methods 0.000 title claims abstract description 24
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- 239000001632 sodium acetate Substances 0.000 claims abstract description 6
- 235000017281 sodium acetate Nutrition 0.000 claims abstract description 6
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 5
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- 238000000605 extraction Methods 0.000 claims description 13
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- 239000013067 intermediate product Substances 0.000 claims description 11
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- 238000001914 filtration Methods 0.000 claims description 9
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- ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 description 1
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- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
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- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
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- 239000008280 blood Substances 0.000 description 1
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- GDTBXPJZTBHREO-UHFFFAOYSA-N bromine Substances BrBr GDTBXPJZTBHREO-UHFFFAOYSA-N 0.000 description 1
- 229910052794 bromium Inorganic materials 0.000 description 1
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- 230000005764 inhibitory process Effects 0.000 description 1
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- PNDPGZBMCMUPRI-UHFFFAOYSA-N iodine Chemical compound II PNDPGZBMCMUPRI-UHFFFAOYSA-N 0.000 description 1
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- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
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- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The present invention discloses a sea mud active component extracting method which comprises the steps of smashing, leaching, centrifugating and condensing. The centrifugating step comprises vaporizing and condensing and obtains sea mud active component extracting solution. The smashing step uses a high pressure airflow smashing form. The leaching step uses pH regulating agent which comprises 1 percent of sodium acetate solution in weight percentage. The condensing step is decompression rotating vaporizing. Compared with the prior art, the method has the advantages of low technical cost, simple operation, low requirement on equipment, and the like, and the sea mud active extracting solution extracted by the method of the present invention has good storage stability and solves the problem of the prior art that active component is hard for retaining for a long time.
Description
Technical field
The present invention relates to a kind of extraction process of active component, relate in particular to a kind of extracting method that is applied to sea mud active component.
Background technology
The composition of sea mud is very complicated, is rich in mineral and trace element such as potassium, sodium, calcium, magnesium, ferrum, chlorine, iodine, bromine and the sulfate of needed by human body and carbonate; Contain a large amount of soil microorganisms and the active metabolite of generation thereof; Also contain vegeto-animal remains such as algae and shell-fish,, form organic substances such as the useful protein of human body skin, aminoacid and polysaccharide through alluvial for many years.
Existing sea mud active component extractive technique adopts the method for organic solvent extraction more, this method adopts the organic solvent bigger to the human body skin zest, easily produce skin allergy, and this method cost is higher, complex process, effective ingredient is difficult to shortcomings such as lasting preservation in the extracting solution.
Summary of the invention
The object of the present invention is to provide a kind of simple and effective method for extracting sea mud active component, have advantages such as cost is low, simple to operate, equipment requirements is low, and through the active extracting solution of the sea mud of this method extraction, have better storage stability, solved the prior art effective ingredient and be difficult to the lasting problem that keeps.
Above-mentioned purpose of the present invention is achieved through the following technical solutions:
A kind of method for extracting sea mud active component may further comprise the steps:
Pulverising step: choose sea mud, it is carried out drying; After the desciccate coarse filtration, be crushed to 50 μ m to 150 μ m, obtain intermediate product;
The lixiviate step: the intermediate product of pulverising step gained is dissolved in the distilled water, and extraction temperature is 45 ℃ to 85 ℃, and adding pH regulator agent adjusting extracting solution pH is 3 to 10, and extraction time is 5min to 60min, obtains the sea mud extracting solution;
Centrifugation step: the sea mud extracting solution of lixiviate step gained is carried out centrifugalize, and the rotating speed of centrifugalize is 3000r/min to 12000r/min, and centrifugation time is 5min to 15min, and keeping centrifuging temperature is 25 ℃ to 35 ℃, obtains the sea mud aqueous solution;
Concentration step: the sea mud aqueous solution of centrifugal gained is carried out evaporation and concentration, obtain the sea mud active component aqueous extract.
Pulverizing the mode that is adopted is that high pressure draught is pulverized.The pH regulator agent comprises that at least mass fraction is 1% sodium acetate solution.Evaporation and concentration is the decompression rotary evaporation.
Because the present invention adopted sea mud, through super-dry and coarse filtration, can remove impurity such as gravel, will filter then sea mud pulverize and obtain the sea mud powder, its exquisite quality; The sea mud powder of pulverizing gained places reactor, adds the distilled water lixiviate through pH regulator, obtains the sea mud crude extract; The sea mud aqueous solution that crude extract is carried out the centrifugalize gained carries out evaporation and concentration, obtains the sea mud active component aqueous extract.
The sea mud active component aqueous extract that makes is carried out the effective ingredient active testing, has good antioxidant activity and the activity of whitening, compared with prior art have advantages such as the technology cost is low, simple to operate, equipment requirements is low, and through the active extracting solution of the sea mud of the present invention's extraction, have better storage stability, solved the prior art effective ingredient and be difficult to the lasting problem that keeps.
Description of drawings
Fig. 1 is a process chart of the present invention.
The specific embodiment
Below in conjunction with embodiment the present invention is described in further detail.
Embodiment 1: with reference to Fig. 1, a kind of method for extracting sea mud active component may further comprise the steps:
Pulverising step: choose sea mud, it is carried out the natural air drying drying; After the desciccate coarse filtration, adopt the high pressure draught grinding mode to be crushed to 50 μ m, obtain intermediate product; The sea mud that picks up from beach or shallow-layer seabeach carries out coarse filtration with modes such as filter clothes after super-dry, can remove impurity such as gravel, will filter then sea mud pulverize and obtain the sea mud powder, its exquisite quality.
The lixiviate step: the intermediate product of pulverising step gained is dissolved in the distilled water, and extraction temperature is 65 ℃, and adopting 1% sodium acetate solution adjusting extracting solution pH is 8, and extraction time is 40min, obtains the sea mud extracting solution;
Centrifugation step: the sea mud extracting solution of lixiviate step gained is carried out centrifugalize, and the rotating speed of centrifugalize is 9000r/min, and centrifugation time is 15min, and keeping centrifuging temperature is 35 ℃, obtains the sea mud aqueous solution;
Concentration step: the sea mud aqueous solution of the centrifugation step gained rotary evaporation that reduces pressure is concentrated, obtain the sea mud active component aqueous extract.
The sea mud active component aqueous extract that makes is carried out the effective ingredient active testing, have good antioxidant activity and the activity of whitening.The plunge into the commercial sea Trolox equivalent of muddy water extracting solution of this condition is 6.50 ± 0.24 μ mol/L, and tyrosinase inhibition rate is 17.72 ± 0.67%.
Table 1 is that the sea mud extract is to Ruditapes philippinarum and mud snail SOD, the activity influence of GSH-Px, result of the test shows, the sea mud active matter that adopts the inventive method to obtain raises 47.43% and 24.40% than matched group respectively to the SOD of the Ruditapes philippinarum muscle of beach growth and the activity of GSH-Px, has significant difference ((P＜0.05), the SOD of the mud snail muscle of beach sea mud growth and the activity of GSH-Px raise 62.59% and 40.38% than matched group respectively, significant difference (P＜0.05) illustrates that the beach sea mud can induce the activity of the SOD of shellfish muscle and GSH-Px to raise.
Table 1 sea mud extract is to the activity influence of Ruditapes philippinarum and mud snail SOD, GSH-Px
The secular deposition of washing away of sea water makes the beach sea mud contain the abundant all kinds of nutrient substance useful to biology.
At first, sea mud is rich in abundant antioxidant vitamins as vitamin A, vitamin E and vitamin C.Studies show that, there are two kinds of antioxidant systems under the biological normal condition, one class is a non-enzyme anti-oxidative defense system, comprises vitamin E and vitamin C, and vitamin E is a kind of very strong antioxidant, be positioned on the cell membrane, before superoxide radical R00 works to the PUFA of mitochondrial membrane with free radical capture and remove, thereby stop peroxide ROOH to generate, the protection cell is avoided infringement, also can prevent the oxidation of vitamin A, vitamin C and ATP simultaneously, guarantee their normal functions at body; Vitamin C is a kind of very strong Reducing agent, catches the strongest hydroxyl free radical OH of peroxidation outside cell membrane, prevents free radical infringement cell, to protect the not oxidized destruction of other materials such as vitamin A, vitamin E and vitamin B.In addition, the precursor substance carotenoid of vitamin A also has certain anti-oxidation function, and it can resist singlet oxygen
1O
2And lipid free radical.Another kind of is enzyme anti-oxidative defense system, and it mainly comprises SOD and GSH-Px, and the former effect is with superoxide radical O
2 -Be converted into hydrogen peroxide H
2O
2, to eliminate the infringement of free radical; The latter can prevent that peroxide further is hydrolyzed to the harmful substance malonaldehyde, and it is reduced to alcohol and water, prevents that cell is damaged by peroxidating.Hence one can see that, and this two classes system of defense realizes antioxidation, the defying age of body, the effect of mutation jointly, and their the synergism anti-oxidation efficacy of enhancing body greatly.
Secondly, sea mud also contains very abundant inorganic mineral element Mg, Sr, Ge, Fe, Zn, Ca.Studies show that these elements have the obvious synergistic effect to the anti-oxidation efficacy of body mostly, Fe is the important component part of hemoglobin, plays a part core in the conveying of oxygen and electronics, also is the important activity composition of Fe-SOD; Zn participates in the catalytic action of the multiple important enzyme of body, and wherein Zn-SOD is one of most important antioxidase in the body; Ge (organic germanium) and polysaccharide are incorporated into human blood, and erythrocytic oxygen carrying capacity is strengthened, and have removing through effects such as free radical, anti peroxidation of lipid, inducement interferon, anticancer, antiviral; Sr has functions such as defying age, carcinogenesis, enhancing radioprotective; Ca and Mg are the important activator of many protease.Therefore the activity of SOD and GSH-Px is very likely obviously strengthened under the influence of the contained mineral element of sea mud.
Once more, sea mud total protease active higher constitutes the stronger antioxidation environment of sea mud under the combined effect of these nutrient substance, and the water-soluble phase system of sea mud has stronger antioxygenic property.
Embodiment 2: a kind of method for extracting sea mud active component may further comprise the steps:
Pulverising step: choose sea mud, it is carried out the natural air drying drying; After the desciccate coarse filtration, adopt the high pressure draught grinding mode to be crushed to 150 μ m, obtain intermediate product; The sea mud that picks up from beach or shallow-layer seabeach carries out coarse filtration with modes such as filter clothes after super-dry, can remove impurity such as gravel, will filter then sea mud pulverize and obtain the sea mud powder, its exquisite quality.
The lixiviate step: the intermediate product of pulverising step gained is dissolved in the distilled water, and extraction temperature is 45 ℃, and adopting 1% sodium acetate solution adjusting extracting solution pH is 8, and extraction time is 45min, obtains the sea mud extracting solution;
Centrifugation step: the sea mud extracting solution of lixiviate step gained is carried out centrifugalize, and the rotating speed of centrifugalize is 10000r/min, and centrifugation time is 15min, and keeping centrifuging temperature is 30 ℃, obtains the sea mud aqueous solution;
Concentration step: the sea mud aqueous solution of the centrifugation step gained rotary evaporation that reduces pressure is concentrated, obtain the sea mud active component aqueous extract.
The sea mud active component aqueous extract that makes is carried out the effective ingredient active testing, have good antioxidant activity and the activity of whitening.
Embodiment 3: a kind of method for extracting sea mud active component may further comprise the steps:
Pulverising step: choose sea mud, it is carried out drying; After the desciccate coarse filtration, adopt the high pressure draught grinding mode to be crushed to 50 μ m, obtain intermediate product; The sea mud that picks up from beach or shallow-layer seabeach carries out coarse filtration with modes such as filter clothes after super-dry, can remove impurity such as gravel, will filter then sea mud pulverize and obtain the sea mud powder, its exquisite quality.
The lixiviate step: the intermediate product of pulverising step gained is dissolved in the distilled water, and extraction temperature is 55 ℃, adopts NaOH and hydrochloric acid to regulate extracting solution pH to 8 and regulates, and extraction time is 60min, obtains the sea mud extracting solution;
Centrifugation step: the sea mud extracting solution of lixiviate step gained is carried out centrifugalize, and the rotating speed of centrifugalize is 12000r/min, and centrifugation time is 5min, and keeping centrifuging temperature is 25 ℃, obtains the sea mud aqueous solution;
Concentration step: the sea mud aqueous solution of the centrifugation step gained rotary evaporation that reduces pressure is concentrated, obtain the sea mud active component aqueous extract.
The sea mud active component aqueous extract that makes is carried out the effective ingredient active testing, have good antioxidant activity and the activity of whitening.
Though the present invention illustrates and describes by the reference preferred embodiment,, those skilled in the art should understand, and all can not depart from the scope of the present invention the design's form and the various variation on the details.
Claims (4)
1, a kind of method for extracting sea mud active component is characterized in that said method comprising the steps of:
Pulverising step: choose sea mud, it is carried out drying; After the desciccate coarse filtration, be crushed to 50 μ m to 150 μ m, obtain intermediate product;
The lixiviate step: the intermediate product of pulverising step gained is dissolved in the distilled water, and extraction temperature is 45 ℃ to 85 ℃, and adding pH regulator agent adjusting extracting solution pH is 3 to 10, and extraction time is 5min to 60min, obtains the sea mud extracting solution;
Centrifugation step: the sea mud extracting solution of lixiviate step gained is carried out centrifugalize, and the rotating speed of centrifugalize is 3000r/min to 12000r/min, and centrifugation time is 5min to 15min, and keeping centrifuging temperature is 25 ℃ to 35 ℃, obtains the sea mud aqueous solution;
Concentration step: the sea mud aqueous solution of centrifugal gained is carried out evaporation and concentration, obtain the sea mud active component aqueous extract.
2, method for extracting sea mud active component as claimed in claim 1 is characterized in that the mode that described pulverizing is adopted is that high pressure draught is pulverized.
3, method for extracting sea mud active component as claimed in claim 1 is characterized in that described pH regulator agent comprises sodium acetate solution at least, and described sodium acetate to account for described intermediate product mass fraction be 1%.
4, method for extracting sea mud active component as claimed in claim 1 is characterized in that described evaporation and concentration is the decompression rotary evaporation.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN200810063429XA CN101327168B (en) | 2008-07-31 | 2008-07-31 | Method for extracting sea mud active component |
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| Application Number | Priority Date | Filing Date | Title |
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| CN200810063429XA CN101327168B (en) | 2008-07-31 | 2008-07-31 | Method for extracting sea mud active component |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103181869A (en) * | 2013-03-28 | 2013-07-03 | 浙江大学 | Multiple-effect skin care lotion prepared from all-natural sea mud extraction and preparation method thereof |
| US20150030558A1 (en) * | 2012-03-01 | 2015-01-29 | Syrinx ZA Intenational Limited | Skincare compositions |
| CN106727241A (en) * | 2016-12-28 | 2017-05-31 | 天津市澧沛佳海水娱乐工程有限公司 | A kind of shampoo prescription containing ooze dissolved matter |
| CN111249767A (en) * | 2020-04-10 | 2020-06-09 | 黄淮学院 | Method for extracting active ingredients of food |
-
2008
- 2008-07-31 CN CN200810063429XA patent/CN101327168B/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150030558A1 (en) * | 2012-03-01 | 2015-01-29 | Syrinx ZA Intenational Limited | Skincare compositions |
| CN103181869A (en) * | 2013-03-28 | 2013-07-03 | 浙江大学 | Multiple-effect skin care lotion prepared from all-natural sea mud extraction and preparation method thereof |
| CN106727241A (en) * | 2016-12-28 | 2017-05-31 | 天津市澧沛佳海水娱乐工程有限公司 | A kind of shampoo prescription containing ooze dissolved matter |
| CN111249767A (en) * | 2020-04-10 | 2020-06-09 | 黄淮学院 | Method for extracting active ingredients of food |
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| Publication number | Publication date |
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| CN101327168B (en) | 2011-08-03 |
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