CN101311272A - Process for preparing acetone, butanol and ethanol by tapioca fermentation - Google Patents
Process for preparing acetone, butanol and ethanol by tapioca fermentation Download PDFInfo
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- CN101311272A CN101311272A CNA2007100412162A CN200710041216A CN101311272A CN 101311272 A CN101311272 A CN 101311272A CN A2007100412162 A CNA2007100412162 A CN A2007100412162A CN 200710041216 A CN200710041216 A CN 200710041216A CN 101311272 A CN101311272 A CN 101311272A
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- tapioca starch
- ammonium acetate
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Abstract
The invention discloses a cassava meal medium and a method for producing acetone, butanol and ethanol by fermentation. The medium of the invention adds ammonium acetate to the cassava meal, so as to improve more than once of the total solvent concentration of products to reach the fermentation level of taking pure cornmeal as raw material. Under the condition of keeping the gross amount and the proportion of the solvent products, the method not only reduces material cost greatly, but also needs no change for the original processing technique, so the method is more suitable for industrial application to the production of the acetone, the ethanol and the butanol.
Description
Technical field
The invention belongs to fermentation technical field, be specifically related to the Tapioca Starch be the Tapioca Starch substratum of raw material and utilize this substratum fermentation production of acetone, butanols, alcoholic acid method.
Background technology
Acetone, butanols and ethanol all are important organic solvent and industrial chemicals, are widely used in spraying paint, explosive, plastics, pharmacy, the plant extracting is got and industry such as synthetic glass, synthetic rubber.Acetone can be used as the solvent of making cellulose acetate film film, plastics and coating, can be used for producing Chemicals such as methylacrylic acid methyl esters (MMA), dihydroxyphenyl propane, aldol(s) again.Butanols is the biofuel of a new generation, compare with prior biological fuel, the ratio of mixture of butanols and gasoline is higher, need not vehicle is transformed, has significant environmental benefit simultaneously, can reduce the discharging of greenhouse gases, therefore, butanols will occupy important proportion in the transport fuel structure in future.Ethanol is good organic solvent and sterilizing agent, and its germicidal action is very fast, and sterilisation effect is reliable, and is little to people's pungency, nontoxic, harmless to article, is used for the clinical sterilization of skin degerming and medicine equipment more.
Usually, and acetone, butanols, ethanol (Acetone-butanol-ethanol, production ABE) is to utilize microbial fermentation, reclaims tunning respectively by distillation then, this fermentation is called the ABE fermentation.The bacterial classification that is used for the ABE fermentation mainly is a clostridium, fermenting raw materials used is grain amyloid raw material, as pure Semen Maydis powder, pure wheat-flour etc., their nutrition is than horn of plenty, need not add any allogenic material in the fermenting process, but cost of material such as corn, wheat is higher, is used for fermentative production ABE solvent not only to have restricted the competitiveness of product in market, also runs counter to the grain security strategy of China.Because in the total cost of ABE fermentative production, raw materials cost occupies important proportion (about 60%), be to influence the key factor that the ABE solvent is produced, therefore, excavate other cheap alternative materials energetically and carry out ABE production, significant for competitive power, the realization Sustainable utilization of resources of raising ABE fermentation industry.
Cassava (Cassava) is a kind of perennial undershrub, is called cassava, wooden sweet potato again, and underground part knot potato is one of the world's three yampi classes (potato, sweet potato, cassava), is the important non-grain class cash crop of China.Tapioca root dry matter content 30～40%, bright potato is starch-containing 32～35%, protein 1～2%, fat 0.3～4.3%, Mierocrystalline cellulose 1～2%, ash content 1%.Tapioca (flour) content height, adaptability is strong, and is water-fast, drought-enduring, can both grow in any soil property, is a kind of rising fermentation raw material of not striving ground with grain.When existing Tapioca Starch substratum is used for fermentative production ABE solvent, yield is far below maize powder medium, this is because concentrations of nutrient such as the protein of cassava, fat are fewer, be unfavorable for microbial growth, therefore can not be directly used in the fermentative production of ABE solvent, must add nutritive substance, but this certainly will will increase raw materials cost, be difficult to adapt to industrial needs, so thereby it is the prerequisite of producing the ABE solvent that the key factor in the fermentation of searching cassava is optimized substratum.
Summary of the invention
The object of the invention is, to existingly be used for fermentation production of acetone, butanols, (Acetone-butanol-ethanol, Tapioca Starch substratum ABE) is optimized ethanol, makes it be suitable for fermentative production ABE solvent.
Another object of the present invention is exactly on the basis of above-mentioned optimization substratum, and providing a kind of is the method that fermenting raw materials is produced the ABE solvent with the Tapioca Starch.
Optimization substratum of the present invention is to add ammonium acetate in the Tapioca Starch substratum.
According to the present invention, the addition of ammonium acetate is 0.05-0.40% in the described substratum, preferably 0.15%.
According to the present invention, the content of Tapioca Starch is 8-10% in the described substratum.
According to the present invention, Tapioca Starch preferably adopts particle diameter less than 100 purpose Tapioca Starchs in the described substratum.
The method that with the Tapioca Starch is fermenting raw materials is produced the ABE solvent of the present invention may further comprise the steps:
(a) with the Tapioca Starch be the preparation of raw material substratum, and add ammonium acetate, boil gelatinization then;
(b) substratum inserts ABE and produces bacterial strain after sterilizing, and cultivates 48-50 hour under 35-40 ℃ condition.
According to method of the present invention, the addition of described ammonium acetate is 0.05-0.40%, preferably 0.15%.
According to method of the present invention, the content of described Tapioca Starch is 8-10%.
According to method of the present invention, described Tapioca Starch adopts particle diameter less than 100 purpose Tapioca Starchs.
According to method of the present invention, it is clostridium acetobutylicum (Clostridium.acetobutylicum) EA 2018 (CCTCC M94061) that described ABE produces bacterial strain.
After measured, adopt method of the present invention, in the substratum that with the Tapioca Starch is raw material, add an amount of ammonium acetate, acetone, butanols, total ethanol solvent strength that the gained fermentation generates can be by being increased to about 18g/L about original 9g/L, yield doubles, and reaches the level that corn fermentation generates solvent; Compare with pure fermentation of corn starch, substratum of the present invention greatly reduces raw materials cost, and does not need original production process is carried out any change, is easier to be adopted by large-scale industrial production.
Embodiment
Below in conjunction with specific embodiment, the invention will be further described.Should be understood that following examples only are used to the present invention is described but not are used to limit scope of the present invention.
According to the present invention, described ABE is meant the abbreviation of acetone, butanols, ethanol (Acetone-butanol-ethanol).
Related percentage concentration in following examples except that indicating especially, is weight percent concentration (w/w).
Embodiment 1, fermentation generates the influence of ABE solvent to Tapioca Starch to add ammonium acetate
Preparation 200ml Tapioca Starch (the south China series cassava that Chinese Academy of Tropical Agricultural Sciences provides) fermention medium, substratum is formed: Tapioca Starch 8-10% (the Tapioca Starch particle diameter is less than 100 orders), ammonium acetate 0.15%.The Tapioca Starch fermention medium for preparing is boiled gelatinization 0.5h, change 500ml over to and shake in the bottle autoclaving.Treat that substratum is cooled to below 40 ℃, insert clostridium acetobutylicum (Clostridium.acetobutylicum) EA 2018 (CCTCC M94061) the bacterium liquid of activated 24h, inoculum size 5%, 35-40 ℃ of fermentation culture.
Simultaneously with the Tapioca Starch fermention medium that do not add ammonium acetate in contrast, under similarity condition, carry out fermentation culture.
To the fermented liquid sampling, sample is centrifugal 10min under the 5000rpm rotating speed at set intervals, collects supernatant liquor.
ABE solvent composition in the supernatant liquor is carried out gas-chromatography (GC) analysis, and chromatographic operational condition is as follows:
Separator column: Alltech EC
TM-WAX (3.0m * 0.32mm * 0.25 μ m)
Column temperature: 85 ℃
Carrier gas: nitrogen
Flow velocity: 5ml/min
Pressure: 0.07MPa
Sample size: 0.5 μ l
Detector: hydrogen flame detector
Two groups of samples are analyzed through gas-chromatography (GC), and wherein the measurement result of acetone, ethanol, butanols and total solvent is as shown in table 1.
Table 1 adds the influence of ammonium acetate to Tapioca Starch fermentation generation ABE solvent
By table 1 result as can be known, when not adding ammonium acetate, speed and output that clostridium acetobutylicum clostridium (Clostridium.acetobutylicum) EA 2018 fermentation Tapioca Starchs generate the ABE solvent are all lower, and the total solvent concentration during fermentation 50h is 9.8g/l; After adding 0.15% ammonium acetate in the Tapioca Starch substratum, total solvent concentration during fermentation 50h reaches and is up to 18.1g/l, double when not adding ammonium acetate, with existing with the Semen Maydis powder to be that fermenting raw materials is produced the yield of ABE suitable, illustrate that adding ammonium acetate has obvious facilitation to Tapioca Starch fermentation generation ABE solvent, can replace existing method, thereby significantly reduce the production cost of ABE with fermenting raw materials such as Semen Maydis powder, wheat-flour production ABE.
Embodiment 2, the ammonium acetate addition generates the influence of ABE solvent to Tapioca Starch fermentation
For further studying of the influence of ammonium acetate addition to Tapioca Starch fermentation generation ABE solvent, press embodiment 1 described method preparation Tapioca Starch substratum, to the ammonium acetate that wherein adds the Different Weight ratio, fermentation condition and product are measured with embodiment 1, experimental result shows that solvent strength peaked when fermentation time was 50h, and the measurement result of acetone, ethanol, butanols and total solvent is as shown in table 2 at this moment.
Table 2 ammonium acetate addition is to the influence of Tapioca Starch fermentation generation ABE solvent
aFermentation time refers to that the product solvent strength peaks the required time.
By table 2 result as can be known, in the ammonium acetate addition is 0.05～0.40% scope, the concentration that Tapioca Starch fermentation generates the ABE total solvent present increase earlier after downward trend to some extent, significantly improve but the concentration that generates solvent in this scope all generates the concentration of solvent when not adding ammonium acetate.Consolidated statement 2 data, Tapioca Starch fermentative production ABE solvent as can be seen, ammonium acetate addition are to reach the highest at 0.15% o'clock, and at this moment, total solvent concentration can reach 18.1g/l, double when not adding ammonium acetate.
Embodiment 3:The cost that Tapioca Starch is optimized substratum and corn culture medium fermentative production ABE solvent compares
Adopting pure Semen Maydis powder is that fermenting raw materials is produced the ABE solvent, and fermentation condition and product are measured with embodiment 1, is that fermenting raw materials production ABE solvent production is made comparisons with fermentative production ABE solvent production and with Tapioca Starch (interpolation ammonium acetate), and the result is as shown in table 3.
The rate ratio of table 3, Tapioca Starch optimization substratum and maize powder medium fermentative production ABE solvent
aFermentation time refers to that the product solvent strength peaks the required time.
By table 3 result as can be known, after in the Tapioca Starch substratum, adding ammonium acetate, the concentration that fermentation generates the ABE total solvent remains basically stable with adopting the concentration with pure maize powder medium fermentation gained solvent, and all about 18g/l, wherein the ratio of three kinds of main component acetone, ethanol and butanols is also approaching.Therefore, the difference of Tapioca Starch and fermentation of corn starch production ABE solvent mainly is the cost of culture medium raw material, compares the price of two kinds of culture medium raw materials, and the result is as shown in table 4.
The cost of table 4, the required cassava of solvent per ton and corn culture medium relatively
By table 4 result as can be known, the employing Tapioca Starch is a fermentation raw material, adding under the situation of ammonium acetate, cost than present be low approximately 600 yuan of/ton solvents of fermentation raw material with pure Semen Maydis powder, greatly reduce raw materials cost.
In a word, it is the ammonium acetate of 0.05-0.40% that the present invention only needs to add weight ratio in the Tapioca Starch substratum, the solvent strength of tunning can be doubled, and reaching with pure Semen Maydis powder is the fermentation level of raw material.Adopt this method, under the situation that keeps product solvent total amount and proportion of products, not only greatly reduce raw materials cost, and do not need original production process is carried out any change, be suitable for the industrial applications of fermentative Production acetone, ethanol, butanols more.
Claims (11)
1, a kind of Tapioca Starch substratum is used for fermentation production of acetone, butanols, ethanol, it is characterized in that, has added ammonium acetate in the Tapioca Starch substratum.
2, substratum as claimed in claim 1 is characterized in that, the content of described ammonium acetate is 0.05-0.40%.
3, substratum as claimed in claim 2 is characterized in that, the content of described ammonium acetate is 0.15%.
4, substratum as claimed in claim 1 is characterized in that, the content of described Tapioca Starch is 8-10%.
5, substratum as claimed in claim 1 is characterized in that, described Tapioca Starch powder footpath is less than 100 orders.
6, a kind of fermentation production of acetone, butanols, alcoholic acid method is characterized in that, this method key step comprises:
(a) with the Tapioca Starch be the preparation of raw material substratum, and add ammonium acetate, boil gelatinization then;
(b) substratum inserts ABE and produces bacterial strain after sterilizing, and cultivates 48-50 hour under 35-40 ℃ condition.
7, as fermentation production of acetone, butanols, alcoholic acid method as described in the claim 6, it is characterized in that the content of described ammonium acetate is 0.05-0.40%.
8, as fermentation production of acetone, butanols, alcoholic acid method as described in the claim 7, it is characterized in that the content of described ammonium acetate is 0.15%.
9, as fermentation production of acetone, butanols, alcoholic acid method as described in the claim 6, it is characterized in that the content of described Tapioca Starch is 8-10%.
As fermentation production of acetone, butanols, alcoholic acid method as described in the claim 6, it is characterized in that 10, described Tapioca Starch powder footpath is less than 100 orders.
As fermentation production of acetone, butanols, alcoholic acid method as described in the claim 6, it is characterized in that 11, it is clostridium acetobutylicum (Clostridium.Acetobutylicum) EA 2018 that described ABE produces bacterial strain.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2007100412162A CN101311272B (en) | 2007-05-24 | 2007-05-24 | Process for preparing acetone, butanol and ethanol by tapioca fermentation |
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| CN2007100412162A CN101311272B (en) | 2007-05-24 | 2007-05-24 | Process for preparing acetone, butanol and ethanol by tapioca fermentation |
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| CN101311272B CN101311272B (en) | 2011-06-22 |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101250562B (en) * | 2008-04-09 | 2011-09-14 | 上海天之冠可再生能源有限公司 | Method for improving yield of acetone, butanol and ethanol produced by fermentation of manioc materials |
| CN102439159A (en) * | 2009-03-09 | 2012-05-02 | 奎特罗斯公司 | Production of fermentive end products from clostridium sp. |
| CN102471780A (en) * | 2009-06-29 | 2012-05-23 | 奎特罗斯公司 | Compositions and methods for improved saccharification of biomass |
| CN101446579B (en) * | 2008-12-26 | 2012-08-08 | 中国科学院微生物研究所 | Method for detecting solvent production by solvent-producing bacteria in high-throughput manner |
| CN102653777A (en) * | 2012-05-08 | 2012-09-05 | 上海中科高等研究院 | Method for producing acetone-butanol-ethanol (ABE) by virtue of fermentation of wheat bran |
| CN102732572A (en) * | 2011-04-01 | 2012-10-17 | 中国科学院过程工程研究所 | Method for producing acetone butanol from fermented cassava |
| CN103255184A (en) * | 2012-02-15 | 2013-08-21 | 百奥泰生物科技(广州)有限公司 | Fermentation medium for producing ansamitocin |
| CN106367349A (en) * | 2016-08-31 | 2017-02-01 | 江苏兴海环保科技有限公司 | Preparation method for cultivating microbial nutrient medium |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100384992C (en) * | 2005-05-12 | 2008-04-30 | 江南大学 | Method for improving stability of alkaline pectase in cotton spinning and refining process |
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2007
- 2007-05-24 CN CN2007100412162A patent/CN101311272B/en not_active Expired - Fee Related
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101250562B (en) * | 2008-04-09 | 2011-09-14 | 上海天之冠可再生能源有限公司 | Method for improving yield of acetone, butanol and ethanol produced by fermentation of manioc materials |
| CN101446579B (en) * | 2008-12-26 | 2012-08-08 | 中国科学院微生物研究所 | Method for detecting solvent production by solvent-producing bacteria in high-throughput manner |
| CN102439159A (en) * | 2009-03-09 | 2012-05-02 | 奎特罗斯公司 | Production of fermentive end products from clostridium sp. |
| CN102471780A (en) * | 2009-06-29 | 2012-05-23 | 奎特罗斯公司 | Compositions and methods for improved saccharification of biomass |
| CN102732572A (en) * | 2011-04-01 | 2012-10-17 | 中国科学院过程工程研究所 | Method for producing acetone butanol from fermented cassava |
| CN103255184A (en) * | 2012-02-15 | 2013-08-21 | 百奥泰生物科技(广州)有限公司 | Fermentation medium for producing ansamitocin |
| CN103255184B (en) * | 2012-02-15 | 2016-01-27 | 百奥泰生物科技(广州)有限公司 | Produce the fermention medium of ansamitocin |
| CN102653777A (en) * | 2012-05-08 | 2012-09-05 | 上海中科高等研究院 | Method for producing acetone-butanol-ethanol (ABE) by virtue of fermentation of wheat bran |
| CN106367349A (en) * | 2016-08-31 | 2017-02-01 | 江苏兴海环保科技有限公司 | Preparation method for cultivating microbial nutrient medium |
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| CN101311272B (en) | 2011-06-22 |
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