CN101294975B - Grouping toxicology analyzer - Google Patents
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- CN101294975B CN101294975B CN 200810115220 CN200810115220A CN101294975B CN 101294975 B CN101294975 B CN 101294975B CN 200810115220 CN200810115220 CN 200810115220 CN 200810115220 A CN200810115220 A CN 200810115220A CN 101294975 B CN101294975 B CN 101294975B
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Abstract
The invention belongs to the analytical and testing instrument field and relates to a development of a grouping toxicology analyzer; the device consists of six modules of a component separating module, a component collecting module, an automatic mechanical arm, a solution processing platform, a compound measuring module and a data processing and control system, wherein, the component collecting module, the automatic mechanical arm and the solution processing platform are arranged in a superclean bench so as to keep the sterile environment; the device is a new analytical system, can complete the relative complex work of three parts of sample application of sample separation, component analysis and bioactivity analysis with high flux on line, thus reducing the dependence on operators greatly, improving the related work efficiency and the reliability of evaluation results at the same time and having wide application prospect in the aspects of the development of toxicology of environmental pollutants, the development of compound metabolites, the toxicity evaluation of industrial products, the screening of drugs and the analysis of effective components, etc.
Description
Technical field
Patent of the present invention belongs to the analytical and testing instrument field, relates to a kind of development of grouping toxicology analyzer.This device can be completed separation evaluation and the evaluated biological activity that respectively forms compound in complex system by automatic on-line, has wide practical use at the aspects such as toxicity assessment, drug screening and analysis of effective component of the toxicologic study of environmental contaminants, the research of compound metabolic product, industrial goods.
Background technology
At present, biologically active and the complex effect evaluation thereof that respectively forms compound in complex system generally comprised three steps: (1) is divided into a plurality of components according to the difference of physics or chemical property with combined pollutant.Differentiate the toxicity of each component with bioassay method, find out and have bioactive component; (2) further separate having bioactive component, screening has bioactive concrete component or compound, and it is carried out qualitative and quantitative analysis; (3) comprehensive organism is measured and chemical analysis results, and the intoxicating factor is quantitatively reached correlation analysis.This scheme operation is very loaded down with trivial details, and mostly is off-line operation, not only wastes time and energy, and also higher to operating personnel's skill level and experience requirement, the different operating person may draw different results.Therefore, we have developed " grouping toxicology analyzer ", this device high flux is online completed the relative numerous and diverse work of point sample three parts of sample separation, component analysis and Analysis on Biological Activity, greatly alleviated the dependence to operating personnel, improve simultaneously the efficient of related work and the reliability of evaluation result, also there is no at present similar specialized equipment both at home and abroad.
Summary of the invention
The purpose of patent of the present invention is to provide a kind of grouping toxicology analyzer, and this device high flux is online completed the relative numerous and diverse work of point sample three parts of the separation that respectively forms compound in complex system, component analysis and evaluated biological activity.
Patent of the present invention realizes by following technical proposals.It comprises six main modular: component separation module 1, component collection module 2, robotic arm 3, solution-treated platform 4, compound determination module 5, data are processed and control system 6.Wherein component collection module 2, robotic arm 3 and solution-treated platform 4 are placed in superclean bench 7, to keep aseptic environment.8 is special test tube rack (12.8cm length * 8.6cm is wide), and its size is identical with common 96 orifice plates, is convenient to automation mechanized operation, places the test tube of 10mL in test tube rack 8.9 are the storage grillage, in order to deposit standby or to handle 96 orifice plates well, and also can holding test tubes frame 8.Chromatographic resolution module 1 is comprised of high-pressure pump, preparative chromatography post, analysis chromatographic column, proportional valve, transfer valve, mainly completes the separation of multiple compounds.Connect by polyfluortetraethylene pipe between separation module 1 and collection module 2, the component solution that collection module 2 is collected is transferred to solution-treated module 4 by robotic arm and carries out point sample.Compound determination module 5 is comprised of triple quadrupole bar tandem mass spectrum (MS/MS) detecting device and liquid chromatography UV-detector, be connected with separation module 1 by chromatogram PEEK (polyetheretherketone) pipe, connect by diverting valve between two detecting devices, solution can enter respectively two detecting devices according to a certain percentage.Separation module 1, component collection module 2, robotic arm 3, solution-treated platform 4 and compound determination module 5 are processed with control system 6 by data and are controlled, whole system can be completed operations automatically according to preset program, and resulting data are processed with control system 6 by data and recorded and process.
The advantage of patent of the present invention is: (1) high flux online completes the loaded down with trivial details operation of point sample three parts of sample separation, component analysis and evaluated biological activity, and work efficiency improves greatly, has also avoided the error of bringing due to manual operation simultaneously; (2) " intelligence " by computer-controlled component is collected and point sample, can in the situation that significantly do not increase and give birth to test sample product quantity, realize the evaluation of compound interphase interaction; (3) whole device is comprised of disparate modules, can carry out multiple combination, realizes the function of various complexity.Also can make up as required and configure separation module 1 and mensuration module 5.(4) component collection module 2, robotic arm 3 and solution-treated platform 4 are placed in superclean bench, and whole operating under aseptic environment completed, and the evaluated biological activity result is more reliable.
Description of drawings
Accompanying drawing is the grouping toxicology analyzer structural representation.
Embodiment
Now by reference to the accompanying drawings the embodiment of patent of the present invention is described in detail.At first sample to be analyzed is a plurality of components through the rough segmentation of preparative chromatography post in component separation module 1, and each component is collected module 2 and is collected in the 10mL test tube and (is placed in special test tube rack 8).Test tube rack 8 is transferred on solution-treated platform 4 by robotic arm 3, is placed in advance on the plate position of design.Then, robotic arm 3 takes out 96 blank orifice plates to solution-treated platform 4 from storage grillage 9, and solution-treated platform 4 is completed distribution and the point sample of solution, cell etc.Put back to after 96 orifice plates after point sample is completed are added a cover by robotic arm 3 on storage grillage 9 again, carry out biological activity determination by artificial unified the taking-up after program is completed.Remaining component solution is also put back in component separation module 2 by robotic arm, for bioactive component is arranged, switches by valve the stratographic analysis post that enters in separation module 1 and separates with compound determination module 5 and identify.If these components are still potpourri, can take these components as sample, repeat above-mentioned process according to requirement of experiment and actual conditions, component is further segmented.To the research of component interphase interaction, except the design orthogonal experiment, can also according to existing result of study and Relational database, control sample injector, to the sample composition independent assortment in each hole of culture plate.
Claims (3)
1. a grouping toxicology analyzer, is characterized in that, processed with the large module of control system six by component separation module, component collection module, robotic arm, solution-treated platform, compound determination module, data to form; The component separation module is comprised of high-pressure pump, preparative chromatography post, analysis chromatographic column, proportional valve, transfer valve, mainly completes the separation of multiple compounds; Connect by polyfluortetraethylene pipe between component separation module and component collection module, the component solution that the component collection module is collected is transferred to the solution-treated platform by mechanical arm and carries out point sample, the compound determination module is comprised of triple quadrupole rods tandem mass spectrometry detecting devices and liquid chromatography UV-detector, be connected with the component separation module by chromatogram PEEK pipe, connect by diverting valve between triple quadrupole rods tandem mass spectrometry detecting devices and liquid chromatography UV-detector, solution enters respectively two detecting devices according to a certain percentage; Component separation module, component collection module, robotic arm, solution-treated platform and compound determination module are processed with control system by data and are controlled, whole system is completed operations automatically according to preset program, and resulting data are processed with control system by data and recorded and process.
2. according to the described device of right 1, it is characterized in that, the test tube rack of employing, long 12.8cm, wide 8.6cm, the test tube of built-in 10mL is collected the component solution that separation module separates.
3. according to the described device of right 1, it is characterized in that, component collection module, robotic arm and solution-treated platform are placed in superclean bench, keep aseptic environment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200810115220 CN101294975B (en) | 2008-06-19 | 2008-06-19 | Grouping toxicology analyzer |
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| Application Number | Priority Date | Filing Date | Title |
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| CN 200810115220 CN101294975B (en) | 2008-06-19 | 2008-06-19 | Grouping toxicology analyzer |
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| CN101294975A CN101294975A (en) | 2008-10-29 |
| CN101294975B true CN101294975B (en) | 2013-06-05 |
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| CN 200810115220 Active CN101294975B (en) | 2008-06-19 | 2008-06-19 | Grouping toxicology analyzer |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103487531B (en) * | 2013-01-28 | 2015-11-25 | 中国药科大学 | A kind of compound library high efficiency preparation method being applicable to natural products high flux screening |
| CN107505439A (en) * | 2017-07-27 | 2017-12-22 | 马鹏飞 | A kind of medicine sterile detector based on robot motion's technology |
| CA3109016A1 (en) * | 2019-07-17 | 2021-01-21 | Research Center For Eco-Environmental Sciences, Chinese Academy Of Sciences | Integrated machines and methods for performing fully-automated biological evaluation and chemical analysis |
| CN110333364B (en) * | 2019-07-17 | 2020-05-12 | 中国科学院生态环境研究中心 | Full-automatic biological evaluation and chemical analysis all-in-one machine and method |
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| CN1289922A (en) * | 2000-11-21 | 2001-04-04 | 中国人民解放军军事医学科学院放射医学研究所 | Biochemical Process for preparing liquid-phase chromatograph |
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| CN1598568A (en) * | 2003-09-19 | 2005-03-23 | 深圳清华大学研究院 | Array continuous preparing chromatography system and using method thereof |
| JP3864876B2 (en) * | 2002-09-04 | 2007-01-10 | 株式会社島津製作所 | Preparative device and preparative liquid chromatograph |
| CN1912627A (en) * | 2006-08-23 | 2007-02-14 | 陕西西大北美基因股份有限公司 | Blood treatment working station based on micronano magnetic particle and its control method |
| JP2007183173A (en) * | 2006-01-06 | 2007-07-19 | Shimadzu Corp | Preparative chromatograph device |
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| CN1289922A (en) * | 2000-11-21 | 2001-04-04 | 中国人民解放军军事医学科学院放射医学研究所 | Biochemical Process for preparing liquid-phase chromatograph |
| CN2476022Y (en) * | 2001-04-20 | 2002-02-06 | 阎超 | Multichannel pressure capillary tube electrochromatographic biological sample analyser |
| JP3864876B2 (en) * | 2002-09-04 | 2007-01-10 | 株式会社島津製作所 | Preparative device and preparative liquid chromatograph |
| CN1598568A (en) * | 2003-09-19 | 2005-03-23 | 深圳清华大学研究院 | Array continuous preparing chromatography system and using method thereof |
| JP2007183173A (en) * | 2006-01-06 | 2007-07-19 | Shimadzu Corp | Preparative chromatograph device |
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| 史建波, 廖春阳, 王亚伟, 江桂斌.气相色谱和原子荧光联用测定生物和沉积物样品中甲基汞.《光谱学与光谱分析》.2006,第26卷(第2期), * |
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