CN101285696B - Volume metering method - Google Patents

Volume metering method Download PDF

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CN101285696B
CN101285696B CN2007100738561A CN200710073856A CN101285696B CN 101285696 B CN101285696 B CN 101285696B CN 2007100738561 A CN2007100738561 A CN 2007100738561A CN 200710073856 A CN200710073856 A CN 200710073856A CN 101285696 B CN101285696 B CN 101285696B
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liquid
volume
vacuum chamber
negative pressure
volume metering
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CN101285696A (en
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赵丙强
甘泉
孙承伟
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Shenzhen Mindray Scientific Co Ltd
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Shenzhen Mindray Bio Medical Electronics Co Ltd
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Abstract

The invention discloses a volume metering method, comprising the steps of generating negative pressure, emptying a volume metering pipe and metering volume, wherein the step of generating negative pressure is to generate negative pressure in a vacuum chamber through an emptying device; the step of emptying the volume metering pipe is to empty the liquid in the volume metering pipe under the action of the negative pressure of the vacuum chamber through the emptying device, and to keep the lower end of the volume metering pipe communicated with negative pressure all the time in the process of emptying the volume metering pipe; the step of metering volume is to meter the volume of the liquid to be metered through a volume metering device. The adoption of the volume metering method in the technical proposal of the invention can effectively prevent residual liquid from gathering on a pipe joint at the lower end of the volume metering pipe and even producing a liquid film in the prior art, thereby ensuring that the volume metering pipe is emptied more thoroughly, so as to avoid producing bubbles in the beginning stage of counting and effectively eliminate the bubbles having adverse effect on measurement results.

Description

A kind of volume metering method
Technical field
The present invention relates to cell detection and analysis technical field, particularly a kind of volume metering method.
Background technology
The utilization impedance method is carried out the blood cell analyzer of cell detection, needs with dilution a certain amount of blood sample to be carried out the dilution of designated ratio earlier, in order to measure WBC (leucocyte), RBC (red blood cell), PLT (blood platelet) and HGB parameters such as (haemoglobins).The diluted sample of WBC, RBC counting chamber is under suction function, and the counting micropore by separately (is inserted in the both sides of counting hole in the liquid with electrode, has set up an electric field environment around the both sides of counting hole respectively.The character non-conducting according to haemocyte, when cell passes through counting hole, to cause the variation of impedance, the size of impedance variation is directly proportional with cell volume), enter the volumescope buret through piping, and by two photoelectric sensors, the liquid measure between these two photoelectric sensors is exactly the specimen amount of instrumental analysis counting.
Existing volume metering mechanism, its liquid road layout as shown in Figure 1.Mainly comprise counting chamber assembly 13, volume metering assembly and emptier.Wherein counting chamber assembly 13 comprises counting chamber forebay 14, counting chamber after-bay 15 and jewel hole 16.Counting chamber forebay 14 connects second liquid pump 17 by the 4th two-way valve 5, is used to prepare the sample to be tested liquid of certain dilution ratio.Counting chamber after-bay 15 is used to store the sample liquid that flows into from the forebay, and counting chamber after-bay 15 also connects dilution liquid pool 18 by the 3rd two-way valve 4.Jewel hole 16 is used to detect cell number and size between counting chamber forebay 14 and counting chamber after-bay 15.The volume metering assembly comprises that volumescope template 9 and setting volume metering thereon begin sensor 10, finish sensor 11 and volumescope buret 12.Volumescope buret 12 vertically be arranged in parallel with volumescope template 9, and two sensors lay respectively at the top and bottom of volumescope buret 9, and the liquid measure between them is exactly the specimen amount that instrumental analysis detects.Emptier comprises vacuum chamber 8, first T-valve 2, second two-way valve 3, the 5th two-way valve 6, first liquid pump 7, air strainer 19, press detection sensor 20 and threeway 21.Wherein vacuum chamber 8 connects first liquid pump 7 by the 5th two-way valve 6; Vacuum chamber 8 also connects the common port of first T-valve 2.The normal-closed end of first T-valve 2 connects threeway 21, and Chang Kaiduan connects the upper end of volume gauge line 12; The upper end of volumescope buret 12 also connects air strainer 19 by second two-way valve 3.Two other port of threeway 21 also connects the lower end and first two-way valve 1 of volume gauge line 12 respectively; An other end connection count pond after-bay 15 of first two-way valve 1.Press detection sensor 20 is arranged on the vacuum chamber 8, is used for the pressure in the vacuum chamber 8 is monitored.
Fig. 2 is counting and volumescope buret evacuation flow journey figure, and as shown in Figure 2, the common counting process of blood cell analyzer comprises: the counting chamber forebay is cleaned, and sample is prepared, and volume metering and counting clean.
(1) forebay, Cleaning count pond: before sample is prepared, clean leucocyte and red blood cell count(RBC) forebay, guarantee that counting chamber cleans up, do not have problems such as carrying pollution; Realize cleaning the discharging of forebay, back waste liquid by operation valve 5, waste drains pump 17.
(2) sample is prepared: the blood sample that will dilute is by a certain percentage squeezed in the counting chamber forebay 14, adds the reagent mixing;
(3) volume metering and counting: in vacuum chamber 8, set up certain negative pressure by operation valve 6, liquid pump 7, make volumescope buret 12 be communicated with vacuum chamber 8 and air strainer 19 then, because the air strainer 19 outer atmosphere that connect, utilize the difference of negative pressure in ambient atmosphere and the vacuum chamber 8 that the liquid in the volumescope buret is discharged into vacuum chamber 8, close first T-valve 2 and second two-way valve 3 then.After 12 emptyings of volumescope buret, in vacuum chamber 8, set up under the prerequisite of stablizing negative pressure; Open first two-way valve 1, make the measurement sample in the counting chamber forebay 14 flow into counting chamber after-bay 15 by jewel hole 16 (detecting the cell number and the impulse magnitude that flow through the jewel hole under the certain volume), after dilution in the counting chamber after-bay 15 mixes, successively flow through first two-way valve 1, volumescope buret 12, (successively detect and finish sensor 11 by volume detection beginning sensor 10, volume from the bottom up) finishes volume metering.
(4) clean: counting finishes, and counting chamber forebay 14, counting chamber after-bay 15, volumescope buret 12 are cleaned, and guarantees can not produce pollution to count next time.
In the prior art, the emptying of volumescope buret comprises: set up negative pressure, evacuation volume gauge line and carry out the process of volume metering.
Detailed process is described below: at first set up negative pressure, open the 5th two-way valve 6, use first liquid pump 7 to set up one negative pressure in vacuum chamber 8.Evacuation volume gauge line then, open first T-valve 2 (making common port C be communicated with) and second two-way valve, 3 certain hours (as 1 second) simultaneously with normal-closed end NC, because the air strainer 19 outer atmosphere that connect, after opening, second two-way valve 3 make vacuum chamber 8 link to each other with ambient atmosphere, in external atmosphere pressure and vacuum chamber 8 under certain suction function, liquid in the volumescope buret 12 is entered vacuum chamber 8 rapidly, close first T-valve 2 and second two-way valve 3 then; For better evacuation volume gauge line 12, switch first T-valve 2 and second two-way valve are more than 3 time repeatedly.Carry out volume metering, open first two-way valve 1, make flow of liquid in the counting chamber after-bay 15 toward volumescope buret 12, liquid level is successively through beginning sensor 10 with finish sensor 11, and then finishes volume metering.Can utilize first liquid pump 7 with outside the liquid discharge machine in the vacuum chamber 8 at last.
Fig. 3 A-3D is the volumescope spirogram in the counting process, the jewel hole 16 that sample flow after a certain amount of dilution is crossed between counting chamber forebay 14 and the counting chamber after-bay 15 is counted, the rear end is measured the liquid volume that flows through jewel hole 16 by a system that is made up of photoelectric sensor and gauge line (volumescope buret), when liquid level passes through the beginning sensor 10 of volumescope buret 12 bottoms, produce an electric signal, counting process begins; When liquid level arrives the end sensor 11 on top, produce an electric signal again, counting process finishes.If in this process, bubble or other abnormal mobility status are arranged in the liquid road, the result of volume metering will be affected, and then have influence on the accuracy of counting; Therefore whether the emptying of volumescope buret is thorough, is the key that guarantees the counting accuracy.
Volumescope buret 12 emptying hydrops problems as shown in Figure 4, the generation of bubble is owing to 12 emptyings of volumescope buret thoroughly do not cause.Adopt after the refluence, emptying is arranged from top to bottom, and counting is meter from the bottom up.After the emptying, the droplet that remains on the tube wall can gather volumescope buret lower end, finally forms liquid film 25 at threeway 21 place.Because emptying does not thoroughly cause the formation liquid film, when the counting beginning, these remaining liquid will form bubble, influence the accuracy of volume metering.
When volume metering began, when solenoid valve was opened, because the sudden change of liquid internal pressure power causes on the liquid and jump that if liquid is piled up at volumescope buret lower end threeway head place, jumping may produce bubble on the liquid so, and in a single day bubble broke, and will have influence on count results; In addition, jumping itself may make liquid directly skip the beginning sensor on the big quantity of fluid, thereby influence counting accuracy.
At present, 12 emptyings of volumescope buret are mainly adopted and are set up a bigger big pressure reduction of negative pressure formation, make that the pressure of at every turn opening electromagnetism first T-valve 2, second two-way valve, 3 evacuation volume gauge lines 12 is bigger.In addition, adopting repeatedly repeatedly evacuation volume gauge line 12 also is a mode.When 12 emptyings of volumescope buret are not thorough, assemble as volumescope buret 12 lower end threeways 21 residual liquid, bubble in jumping on the liquid when possible occurrence count begins, counting, measure the inaccurate problems such as (if begin sensor 10 and finish residual liquid between the sensor 11, that detect sample volume at every turn and will diminish) of liquid volume.In order thoroughly to solve air bubble problem, must be when liquid thoroughly drains and guarantees the counting beginning in the volumescope buret 12 lower end threeways 21 liquid do not go up and jump, we can say that this remains the halfway leftover problem of volumescope buret emptying.
The existing blood cell analyzer that adopts the liquid type of flow from the bottom to top, in the volume metering process, if the emptying of volumescope buret is not thorough, big quantity of fluid is piled up at the place as volumescope buret lower end threeway head, the problem that will occur bubble during the counting beginning, make volume metering inaccurate, and then make the count results variation.Though at present can be by increasing the pressure reduction and the mode of emptying repeatedly, further evacuation volume gauge line, the possibility that produces bubble in the counting process is reduced to a certain degree, but can not thoroughly deals with problems, and prior art can not solve the problem of jumping on the liquid when counting beginning.
Summary of the invention
The objective of the invention is to solve the inaccurate problem of volume metering in the blood cell analyzer, specifically be to solve the emptying of volumescope buret thoroughly not produce bubble, and then solve the inaccurate problem of blood cell analyzer volume metering.
To achieve the above object of the invention, the technical scheme that the present invention takes is: a kind of volume metering method comprises the step that produces negative pressure, evacuation volume gauge line, carries out volume metering; Wherein producing negative pressure is to produce negative pressure by emptier in vacuum chamber; The evacuation volume gauge line is to make by emptier liquid emptying under the effect of vacuum chamber negative pressure in the volumescope buret in evacuation volume gauge line process, to keep volumescope buret lower end to be communicated with negative pressure always; Carry out volume metering and be measuring by the volume that volume metering mechanism is treated metering liquid.
Further, emptying is by the control emptier, makes volumescope buret upper end connect ambient atmosphere, and the lower end connects the negative pressure of vacuum chamber, makes that the liquid in the volumescope buret flows to vacuum chamber under the effect of vacuum chamber negative pressure and external atmosphere pressure.
Further, flow to control, make and treat that metering liquid directly flows to earlier vacuum chamber in the incipient stage of carrying out volume metering, wait to flow through after the pipe adapter of volumescope buret lower end, control emptier again, make it flow to the volumescope buret, to carry out cubing.
Further, flowing to control is by the control emptier, makes negative pressure link to each other with the lower end of volumescope buret earlier, treat that metering liquid flows through after the pipe adapter of volumescope buret lower end, negative pressure is switched to volumescope buret upper end and realization again.
Further, before carrying out volume metering, repeatedly repeat to produce the step of negative pressure and evacuation volume gauge line.
Further again, also comprise the emptying vacuum chamber; By the control emptier, with the liquid emptying in the vacuum chamber.
Adopt a kind of volume metering method of technical solution of the present invention, because in evacuation volume gauge line process, keep volumescope buret lower end to link to each other with negative pressure always, the liquid that makes the pipe adapter place pile up flows to the vacuum chamber of negative pressure, can effectively avoid in the prior art liquid to assemble at pipe adapter place, volumescope buret lower end, even the phenomenon of generation liquid film, this just makes that the emptying of volumescope buret is more thorough, thereby avoid producing bubble, effectively eliminated the bubble that measurement result is had a negative impact in the counting incipient stage.
Owing to, keep volumescope buret lower end and link to each other with the negative pressure of vacuum chamber in the incipient stage of carrying out volume metering, just make and treat that metering liquid flows to vacuum chamber earlier, treat to make it flow to the volumescope buret again after the pipe adapter of its volumescope buret lower end of flowing through; Because pressure jump causes the situation that liquid level is beated, elimination is beated to the adverse effect of measurement result because of liquid level when having avoided solenoid valve to open.
Because before carrying out volume metering, evacuation volume gauge line repeatedly can be better with the hydrops emptying in the volumescope buret.
Because after each volume metering is finished, the process of an emptying vacuum chamber is arranged, the equipment that makes can both have a good original state before each run, reduce rate of breakdown.
Description of drawings
Fig. 1 is the liquid road schematic layout pattern of volume metering mechanism.
Fig. 2 is counting and volume metering process flow diagram.
Fig. 3 is the volumescope spirogram in the counting process.
Wherein:
Fig. 3 A shows counting beginning evacuation volume gauge line;
Fig. 3 B shows that liquid level rises from bottom to top in the counting process;
Fig. 3 C shows liquid level through the beginning sensor, the counting beginning;
Fig. 3 D shows liquid level through finishing sensor, and counting finishes.
Fig. 4 is a volumescope buret hydrops problem synoptic diagram.
Fig. 5 is the volumescope buret evacuation flow journey figure of the specific embodiment of the invention one.
Fig. 6 is the volume metering process flow diagram of the specific embodiment of the invention one.
Fig. 7 is the liquid road schematic layout pattern of the specific embodiment of the invention two.
Fig. 8 is the liquid road schematic layout pattern of the specific embodiment of the invention three.
Embodiment
Embodiment one
The volume metering mechanism of present embodiment, its liquid road layout is referring to shown in Figure 1.The present invention guarantees the thorough emptying of volumescope buret by the householder method of new volumescope buret emptying; Simultaneously, even guarantee that with new counting beginning mode counting has liquid remnants when beginning, this liquid is not also gone up and is jumped, thereby guarantees the performance of no aeration counting in the counting process.
The flow process of concrete evacuation volume gauge line and volume metering comprises the steps: shown in Fig. 5,6
Step 1: set up negative pressure, open the 5th two-way valve 6, first liquid pump 7 is set up certain negative pressure in vacuum chamber 8, press detection sensor 20 is monitored the pressure in the vacuum chamber 8 always;
Step 2: evacuation volume gauge line, open second two-way valve 3, first T-valve 2 (switches to normal-closed end NC, common port C is communicated with Chang Kaiduan NC) certain hour, because the outer atmosphere that connect of air strainer 19, the liquid in the volumescope buret 12 will enter in the vacuum chamber 8 under the effect of the negative pressure of vacuum chamber 8 and external atmosphere pressure.Close second two-way valve 3, and keep first T-valve 2 to open always, make threeway 21 place's residual liquids flow to vacuum chamber 8 through first T-valve 2.
Step 3: emptying repeatedly, promptly step 2 is many times, makes that 12 emptyings of volumescope buret are more thorough, and threeway 21 place do not have abundant residues liquid, thereby guarantee no aeration counting properties in the counting process.
Step 4: volume metering, set up at vacuum chamber under the prerequisite of certain stable negative pressure, at first keep first T-valve 2 to open, promptly the normal-closed end of first T-valve 2 and common port are communicated with; Open first two-way valve 1 then, make the liquid in the counting chamber after-bay 15 under the effect of negative pressure, flow to vacuum chamber 8, treat after its threeway of flowing through volumescope buret 12 lower ends 21, close first T-valve 2, the negative pressure that connects volume gauge line 12 is so just switched to by its lower end of original connection and connects its upper end, liquid to be measured will flow to the volume metering pipe and carry out volume metering under the effect of negative pressure, open counting assembly in the time of volume metering and count.Owing to enter the metering liquid for the treatment of of volumescope buret 12, that has caused when having avoided opening first two-way valve 1 treats the sudden change of metering liquid internal pressure, thereby can not produce on the liquid level and jump.
Press detection sensor 20 is monitored the pressure in the vacuum chamber 8 always, if the negative pressure deficiency, also can be before each repeating step 2 repeating step 1, to guarantee in the vacuum chamber 8 enough negative pressure always being arranged.
The process that can also comprise the emptying vacuum chamber after volume metering is finished utilizes liquid pump 7 with the liquid emptying in the vacuum chamber 8.
The purging method that this is routine, in the process of evacuation volume gauge line, after opening first T-valve 2 and second two-way valve, 3 certain hours, only close second two-way valve 3, rather than adopt the prior art scheme that first T-valve 2 and second two-way valve 3 are all closed, and so just make the negative pressure of lower end one direct sum vacuum chamber 8 of volumescope buret 12 be communicated with, the liquid in the volumescope buret 12 can be mobile to vacuum chamber always, just be not easy to pile up, and then improve the emptying effect of volumescope buret 12 at threeway head place.
In this example,, open after first two-way valve 1 in the incipient stage of carrying out volume metering, because the common port of first two-way valve 2 is communicated with normal-closed end, so just make liquid in the counting chamber after-bay 15, in vacuum chamber 8 under the effect of negative pressure, directly flow to vacuum chamber 8 by threeway 21.The front end face for the treatment of liquid stream is by closing first two-way valve 1 after the threeway 21, the common port of first two-way valve 1 is communicated with normal-closed end, make liquid then flow to volumescope buret 12, and beginning volume metering process, the metering liquid internal pressure for the treatment of that has caused when so just having avoided opening first two-way valve 1 is suddenlyd change, thoroughly solved in the prior art problem of beating at volume metering incipient stage liquid level.
Embodiment two
The liquid road layout of present embodiment is actually and has done certain change on the basis of former Fig. 1 as shown in Figure 7, particularly is to use the 6th two-way valve 22 and the 7th two-way valve 23 to replace first T-valve 2 among Fig. 4.Being vacuum chamber 8 connects the threeway 21 of volume gauge lines 12 lower ends by the 7th two-way valve 23, and vacuum chamber 8 also connects the upper end of volume gauge lines 12 by the 6th two-way valve 22.Under this pipe-line layout, can play the identical effect of the present invention equally.
In like manner, the evacuation procedure of present embodiment volumescope buret 12 comprises the steps:
Step 1: open the 5th two-way valve 6 and first liquid pump 7, vacuum chamber 8 is set up the negative pressure of certain pressure;
Step 2 is opened the 7th two-way valve 23, second two-way valve, 3 certain hours as carrying out the emptying of volumescope buret 12 in 1 to 2 second; Close second two-way valve 3, and keep the 7th two-way valve 23 to open always, guarantee that threeway 21 place's residual liquids are drained.Like this, because the 7th two-way valve 23 is opened, volumescope buret 12 lower ends connect negative pressure, and under the suction function in vacuum chamber 8, the liquid that pile up at threeway 21 place will flow to the 7th two-way valve 23.
Repeating step 1, step 2 many times.Can make that like this emptying of volumescope buret is more thorough, threeway 21 place do not have abundant residues liquid, thereby guarantee no aeration counting properties in the counting process.
Set up and stablize under the prerequisite of negative pressure, when the counting beginning, when opening first two-way valve 1, the 6th two-way valve 22, keep the 7th two-way valve 23 unlatching certain hours, such as 0.5 second.Liquid will flow to the 7th two-way valve 23 from first two-way valve 1, close the 7th two-way valve 23 then, and first two-way valve 1 and the 6th two-way valve 22 is opened always, flow direction volumescope buret 12, beginning volume metering.Like this, the problem of having jumped on the liquid when having solved the counting beginning, thus further guarantee the accuracy of volume metering.
Embodiment three
The liquid road layout of present embodiment is actually and has done certain change on the basis of former Fig. 4 as shown in Figure 8, particularly is to use second T-valve 24 to replace second two-way valve 3 among Fig. 4.The common port of second T-valve 24 connects the upper end of volume gauge line 12, and Chang Kaiduan connects ambient atmosphere by air strainer 19, and normal-closed end is connected with the normal-closed end of first T-valve 2.Under this pipe-line layout, can play the identical effect of the present invention equally.
In like manner, the evacuation procedure of present embodiment volumescope buret 12 comprises the steps:
Step 1: open the 5th two-way valve 6 and first liquid pump 7, vacuum chamber 8 is set up the negative pressure of certain pressure;
Step 2 is opened first T-valve 2 and second T-valve, 24 certain hours (such as 1 to 2 second) carry out the emptying of volumescope buret 12; Close second T-valve 24, and keep first T-valve 2 to open always, make the lower end of volumescope buret 12 connect the negative pressure of vacuum chamber 8, the liquid of threeway 21 a places accumulation will flow to first T-valve 2 like this, helps the emptying of volumescope buret 12.
Repeating step 1, step 2 many times.Can make that like this 12 emptyings of volumescope buret are more thorough, threeway 21 place can not form liquid film, thereby guarantee no aeration counting properties in the counting process.
When the counting beginning, guarantee to open first two-way valve 1 and second T-valve 24 simultaneously under the prerequisite of negative regulation in the vacuum chamber 8, and guarantee that first T-valve 2 is opening, liquid will flow to first T-valve 2 from first two-way valve 1.(such as 0.5 second) closes first T-valve 2 after the certain hour, and first two-way valve 1 and second T-valve 24 are opened always, and liquid will flow to volumescope buret 12, the beginning volume metering.Like this, the problem of having jumped on the liquid when having solved the counting beginning, thus further guarantee the accuracy of volume metering.
The present invention can transport in all blood cell analyzers that adopt volume metering from bottom to top.
Above content be in conjunction with concrete preferred implementation to further describing that the present invention did, can not assert that concrete enforcement of the present invention is confined to these explanations.For the general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, can also make some simple deduction or replace, all should be considered as belonging to protection scope of the present invention.

Claims (10)

1. volume metering method that is used for volume metering mechanism, described volume metering mechanism comprises volume metering assembly and emptier, described volume metering assembly comprises the volumescope buret, described emptier comprises vacuum chamber, described volumescope buret connects vacuum chamber by pipe adapter, and described method comprises the steps:
A, set up negative pressure, refer in vacuum chamber, produce negative pressure by emptier;
B, evacuation volume gauge line refer to make liquid emptying under the effect of negative pressure in the volumescope buret by emptier;
C, carry out volume metering: refer to that the volume for the treatment of metering liquid by the volume metering assembly measures;
It is characterized in that in step B evacuation volume gauge line process, volumescope buret lower end keeps being communicated with negative pressure always, the liquid that makes pipe adapter place, gauge line lower end pile up also flows to vacuum chamber under suction function.
2. a kind of volume metering method according to claim 1, the incipient stage that it is characterized in that step C, treat metering liquid and flow to control, make that liquid to be measured at first flows to vacuum chamber, treat that it flows through after volumescope buret lower end and the pipe adapter that vacuum chamber is connected, makes flow direction volumescope buret to be measured again.
3. a kind of volume metering method according to claim 1, it is characterized in that, the detailed process of step B comprises: make volumescope buret upper end connect ambient atmosphere by emptier, the lower end connects negative pressure, makes that the liquid in the volumescope buret flows to negative pressure under the effect of negative pressure and external atmosphere pressure; Disconnect being connected of volumescope buret upper end and ambient atmosphere then, and its lower end keeps always being communicated with negative pressure.
4. a kind of volume metering method according to claim 3, the incipient stage that it is characterized in that step C, treat metering liquid and flow to control, make that liquid to be measured at first flows to vacuum chamber, treat after its pipe adapter that flows through volumescope buret lower end, make flow direction volumescope buret to be measured again.
5. according to claim 2 or 4 described a kind of volume metering methods, it is characterized in that, the detailed process of described flow direction control comprises: make the lower end of volumescope buret link to each other with negative pressure earlier, treat that metering liquid flows through after the pipe adapter of volumescope buret lower end, again negative pressure is switched to volumescope buret upper end.
6. according to arbitrary described a kind of volume metering method in the claim 1~4, it is characterized in that before step C carried out volume metering, repeatedly repeating step A set up negative pressure, step B evacuation volume gauge line.
7. a kind of volume metering method according to claim 6 is characterized in that, also comprises the step of emptying vacuum chamber; Described emptying vacuum chamber is meant, by the control emptier, with the liquid emptying in the vacuum chamber.
8. according to arbitrary described a kind of volume metering method in the claim 1~4, it is characterized in that, also comprise the step of emptying vacuum chamber; Described emptying vacuum chamber is meant, by the control emptier, with the liquid emptying in the vacuum chamber.
9. a kind of volume metering method according to claim 5 is characterized in that, before step C carried out volume metering, repeatedly repeating step A set up negative pressure, step B evacuation volume gauge line.
10. a kind of volume metering method according to claim 9 is characterized in that, also comprises the step of emptying vacuum chamber; Described emptying vacuum chamber is meant, by the control emptier, with the liquid emptying in the vacuum chamber.
CN2007100738561A 2007-04-11 2007-04-11 Volume metering method Active CN101285696B (en)

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CN102213607B (en) * 2011-04-12 2013-07-10 深圳市蓝韵实业有限公司 Liquid volume metering device
CN106370261A (en) * 2016-08-24 2017-02-01 深圳市有为环境科技有限公司 Photoelectric meter with defoaming function and metering method
CN106404098B (en) * 2016-08-30 2019-11-22 深圳市有为环境科技有限公司 A kind of metering system
CN110243749A (en) * 2019-05-29 2019-09-17 中国科学院苏州生物医学工程技术研究所 A kind of blood analysis chips and blood analysis system

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US20070039978A1 (en) * 2005-08-19 2007-02-22 Erich Scheugenpflug Emptying device

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Publication number Priority date Publication date Assignee Title
EP0341438A3 (en) * 1988-05-13 1990-11-28 Abbott Laboratories Pneumatic sensing system
CN2034155U (en) * 1988-08-25 1989-03-15 林子敬 Liquid filling machine for spraying agent bottle
CN2482062Y (en) * 2001-03-12 2002-03-13 石油大学(华东)石油仪器仪表研究所 Fluid controlled piston small flowmeter
CN1394780A (en) * 2002-08-08 2003-02-05 杨关键 Vacuum locomotive and cleaning dust-removing system of parts and components
US20070039978A1 (en) * 2005-08-19 2007-02-22 Erich Scheugenpflug Emptying device

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