CN101269098A - Sanqi fermentation liquor with effect of inhibiting cancer cell and preparation method thereof - Google Patents
Sanqi fermentation liquor with effect of inhibiting cancer cell and preparation method thereof Download PDFInfo
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Abstract
The invention relates to a notoginseng zymotic fluid and the preparation method thereof. The method of fermenting notoginseng is as follows: firstly, lactobacillus is activated; secondly, lactobacillus is inoculated to a notoginseng culture fluid for fermentation to obtain a zymotic fluid; thirdly, the zymotic fluid is centrifugated and a supernatant fluid is obtained. The supernatant fluid obtained through centrifugation is the notoginseng zymotic fluid in the invention. The notoginseng zymotic fluid has an effect on the inhibition of cancer cells.
Description
Technical field
The present invention relates to a kind of anti-cancer composition and preparation method thereof, relate in particular to a kind of anticancer Radix Notoginseng streptococcus acidi lactici fermented solution and preparation method thereof.
Background technology
Cancer is the second largest disease that the whole world is only second to cardiovascular disease, up to now, except the minority cancer, can pass through surgical resection as rectal cancer and breast carcinoma, or leukemia can obtain outside certain therapeutic effect by chemicals, and most cancer is still felt simply helpless when cancer cell metastasis.At present domestic cancer mortality just had a people to die from cancer in average per 14 minutes and 07 seconds up to 26 eight percent.The Therapeutic Method of hepatocarcinoma mainly contains surgical operation, X-ray therapy, immunotherapy, chemical medicinal treatment and ethanol local injection etc. at present.Most of antitumor drug have preferable toxic effect for quick outgrowth cancerous cell (as: leukemia, lymphatic cancer) in human body, but the therapeutic effect of cancer (as: hepatocarcinoma, pulmonary carcinoma) is then not good more slowly for development; In addition, many antitumor drug pair cell selectivitys are relatively poor, and therefore adverse side effects such as the immunosuppressant of merging are often arranged.Therefore, seeking the natural plant composition with active anticancer from traditional Chinese herbal medicine, serves as the new direction of studying at present with auxiliary or replacement Western medicine.
Liver is the organ of intraperitoneal maximum, also is the body of gland of human body inner volume maximum, and its weight accounts for into 2.5% of body weight for humans, has multi-functionals such as metabolism, storage, detoxifcation and dextran albumen.The functional unit of liver is lobule (lobule), is made up of hepatic parenchymal cells and nonparenchymal cell or Interstitial cell.Hepatic parenchymal cells (parenchymal cells) generally is meant hepatocyte (hepatocytes), and nonparenchymal cell then comprises hole endotheliocyte (sinusoids endothelial cells), sternzellen (stellate cells) and depot fat cell (fat-storingcells) etc.Wherein, hepatocyte accounts for the 60-65% of total hepatocyte number, and the hepatocyte cell volume is bigger, so hepatocyte accounts for the 70-80% of liver cumulative volume.
Hepatocarcinoma is the malignant tumor of liver, generally can be divided into two kinds: one for stemming from liver, is called primary hepatocarcinoma, and wherein 60% is to be changed by liver cirrhosis; Two are diffused into the secondary liver cancer of liver for the health other parts.Divide according to morphology, hepatocarcinoma can be divided three classes: block type, nodular type and diffuse type are wherein maximum with nodular type.Divide according to the histology, hepatocarcinoma can be divided into (1) hepatocarcinoma (Hepatocellular carcinoma), promptly is used to the hepatocarcinoma that claims, accounts for 80% of hepatocarcinoma incidence rate; (2) cancer of biliary duct (Cholanglocarcinoma) accounts for 20% of hepatocarcinoma incidence rate, is mainly in the old man; (3) hepatocyte cancer of biliary duct (Mix hepatocholangiocarcinoma) is comparatively rare; (4) liver archeocyte cancer (Hepatoblastoma) is comparatively rare, is mainly in the child.
The method for the treatment of hepatocarcinoma clinically can be divided into: excision (surgical resection) or locality treatment, as alinjection in the percutaneous puncture tumor (percutaneous ethanol injection), radio frequency burn treatment (radiofrequency ablation), cryotherapy (cryotherapy), and microwave solidification treatment (percutaneousmicrowave coagulation) (Carr, 2004; Labont? et al., 2000), the method effect of most of treatment is not obvious.The success rate of operation of liver cancer excision at present has only 10-20%, and the survival rate in 5 years of postoperative is between 20-50%.Even less than 2 centimeters hepatocarcinoma, the probability that recurred in back 5 years in operation also is absolutely.In addition, aspect the use of medicines resistant to liver cancer, the existing most toxicity of medicine are big, specificity is little, and ill effect such as tool immunosuppressant mostly.Doxorubicin (Adriamycin) uses one of medicine for chemotherapy, belong to antibiotics, side effect is for increasing probability (Bristow et al., 1978 of suffering from cardiomyopathy (cardiomyopathy) or heart failure (congestive heartfailure); Minow, 1975), feel sick, vomiting, alopecia, then may cause surrounding tissue local necrosis phenomenon if leak outside.Therefore, except the new medicine of the less side effect of active development, then be to study how to assign to reach the purpose of the liver protecting health, prophylaxis of cancer generation on the other hand by absorbing natural diet one-tenth.
Radix Notoginseng (Panax notoginseng (Burk.) F.H.Chen); the Araliaceae Panax; warm in nature, acrid in the mouth; tradition is used for dissipating blood stasis hemostasis, subduing swelling and relieving pain etc.; be one of traditional rare medicinal herbs of China, be proved at present have analgesia, physiological function such as hepatoprotective, anti-inflammatory, antithrombotic, blood pressure lowering, protection cardiac muscle and antitumor.Its main agents area is a dried roots, You Cheng Ginseng Radix Notoginseng, Radix Notoginseng, Radix Stephaniae Sinicae (Radix Stephaniae Dielsianae) etc.Radix Notoginseng has antithrombotic, blood pressure lowering, protection cardiac muscle, analgesia, anti-inflammatory, resisting fatigue and effect such as protects the liver, and its active substance comprises saponin (dammarane), Radix Notoginseng triglyceride (trilinolein), Radix Notoginseng amino acid (dencichine), Radix Notoginseng polysaccharide (sanchinan-A), ferment (xylanase) and flavonoid.
Radix Notoginseng have hepatoprotective, the treatment hepatopathy effect, in recent years Japan and in state-owned many health promoting products be Main Ingredients and Appearance with the Radix Notoginseng.But the saponin in the Radix Notoginseng can be subjected to the metabolism and the inhalation effects of digestive system, and influences its effect.Therefore, need provide a kind of and can promote that Radix Notoginseng protects the liver, the method for anti-cancer ability.
Summary of the invention
The object of the present invention is to provide a kind of good anticancer compound and preparation method thereof.
For achieving the above object, the invention provides the method for a kind of Radix Notoginseng of fermenting, it comprises: with culture medium activation lactobacillus; Lactobacillus inoculum is fermented to the Radix Notoginseng culture fluid, to obtain fermentation liquid; Centrifugal fermentation liquid is obtained supernatant.
For achieving the above object, the present invention also provides a kind of Radix Notoginseng streptococcus acidi lactici fermented solution, and by method for preparing, it has the ability of anticancer growth.
For achieving the above object, the present invention provides a kind of medical composition that prevents and/or treats cancer in addition, and it comprises: the Radix Notoginseng streptococcus acidi lactici fermented solution by method for preparing of effective dose, and a kind of pharmaceutically acceptable carrier or excipient.
Description of drawings
In order to allow goal of the invention of the present invention, feature and advantage become apparent, in conjunction with the specific embodiment and accompanying drawing, the present invention is further elaborated now, wherein:
Fig. 1 a-1b is depicted as all Radix Notoginseng lactic fermenting products and has the ability that suppresses Hep 3B or Hep G2 cell.
Fig. 2 a-2b is depicted as the contrast that mixes with single lactobacillus anticancer effect.
When Fig. 3 a-3b is depicted as fermentation temperature and is 32 ℃, the anticancer effect of tunning.
When Fig. 3 c-3d is depicted as fermentation temperature and is 37 ℃, the anticancer effect of tunning.
When Fig. 3 e-3f is depicted as fermentation temperature and is 42 ℃, the anticancer effect of tunning.
It is 3.5 o'clock that Fig. 4 a-4b is depicted as pH value, the anticancer effect of tunning.
It is 4.5 o'clock that Fig. 4 c-4d is depicted as pH value, the anticancer effect of tunning.
It is 5.5 o'clock that Fig. 4 e-4f is depicted as pH value, the anticancer effect of tunning.
Fig. 5 shows that Semen sojae atricolor essence gets liquid and can increase antioxidant effect.
Fig. 6 a-6b shows that heat treatment Radix Notoginseng culture fluid can strengthen anticancer effect in advance.
Figure 7 shows that Radix Notoginseng fermented solution is to normal hepatocellular influence.
Figure 8 shows that the influence of Radix Notoginseng fermented solution to the hepatitis B surface antigen.
Figure 9 shows that zooperal result.
The specific embodiment
The invention provides the method for a kind of Radix Notoginseng of fermenting, it comprises: the activation lactobacillus; Lactobacillus inoculum is fermented to the Radix Notoginseng culture fluid, to obtain fermentation liquid; Centrifugal fermentation liquid is obtained supernatant.
At first with culture medium activation lactobacillus, culture medium can be general existing culture medium to fermentation process of the present invention, as MRS or digestible protein-yeast extract (peptone-yeast extract base) etc.Lactobacillus can be streptococcus salivarius thermophilus (Streptococcus salivarius subsp.Thermophilus (BCRC12268)), Switzerland's lactobacillus (Lactobacillus helveticus (BCRC14092)), thunder Man lactobacillus (Lactobacillus rhamnosus GG. (BCRC16000)), lactobacillus acidoilus (Lactobacillusacidophilus (BCRC10695)), luxuriant and rich with fragrance De Shi-Long root fungus (Bifidobacterium longum (BCRC14602)), chain bacillus bifidus (Bifidobacterium catenulatum (BCRC14667)), short in non-moral Salmonella (Bifidobacterium breve (BCRC11846)), two fork lactobacilluss (Bifidobacteriumbifidum (BCRC14615)) or its combination.
Then, above-mentioned at least a lactobacillus inoculum is fermented in the Radix Notoginseng culture fluid.In the present invention, can use single lactobacillus or mixing lactic acid bacteria, be preferably mixing lactic acid bacteria.The composition of Radix Notoginseng culture fluid is the Notoginseng Root of 10-30% (w/w) and the distilled water of 70-90% (w/w), is preferably the Notoginseng Root of 10-15% (w/w) and the distilled water of 85-90% (w/w).Radix Notoginseng is divided into 20,40 or 60 according to the big I of its root wart, in the present invention, preferably can use the Radix Notoginseng more than 40.Fermentation time was preferably 1 to 3 day between 1 to 7 day.Fermentation temperature is preferably 30 to 40 ℃ between 25 to 42 ℃, the best is 35 to 37 ℃.PH value during fermentation is preferably between 3.5 to 4.5 between 3.0 to 5.0.Should be noted in the discussion above that if pH value more than 5.5, then tunning does not just have anticancer effect.In addition, in the process of fermentation, but the agitation as appropriate culture fluid, to shorten fermentation time.
After fermentation is finished, with the high temperature sterilize stuck fermentation, and centrifugal filtration thalline and carbon nitrogen source solid content, obtain tunning of the present invention.
In an embodiment of the invention, can add other active substance (as Semen sojae atricolor or Radix Bupleuri extract etc.) to the Radix Notoginseng culture fluid, increasing the biological activity of tunning, as oxidation resistance etc.
In an embodiment of the invention, heat treatment Radix Notoginseng culture fluid in advance is to increase the anticancer effect of tunning.Heat treatment temperature is preferably in more than 100 ℃ more than 75 ℃.Heat treatment time is preferably 20 to 180 minutes more than 10 minutes.
The present invention also provides a kind of Radix Notoginseng streptococcus acidi lactici fermented solution, is made by above-mentioned fermentation flow process.Radix Notoginseng streptococcus acidi lactici fermented solution of the present invention has the ability of anticancer growth, and suppression ratio reaches more than 60%.Wherein cancerous cell can be: breast cancer cell, prostate gland cancer cell, blood cell, rectum cancer cell, uterus carcinoma cell, ovarian cancer cell, endometrial carcinoma cell, cervical cancer cell, testicular cancer cell, lymphocytic cancer cell, human rhabdomyosarcoma cells, neuroblastoma cell, pancreatic cancer cell, lung carcinoma cell, brain tumor cell, skin cancer cell, stomach cancer cell, hepatoma carcinoma cell, renal cancer cell or nasopharyngeal carcinoma cell are preferably hepatoma carcinoma cell.
Compositions of the present invention has tight security, but the growth of Radix Notoginseng lactic fermentation liquid anticancer of the present invention, and less to Normocellular toxicity.Radix Notoginseng fermented solution of the present invention is to mice hepatocellular LD of first generation
50More than 900 μ g/ml, be preferably in more than the 970 μ g/ml.
But except the growth of anticancer, Radix Notoginseng lactic fermentation liquid of the present invention also can suppress hepatitis B surface antigen (HBsAg), and it suppresses effect increases along with fermentation time, and suppression ratio can reach more than 80%.
In addition, the present invention provides a kind of medical composition that prevents and/or treats cancer in addition, and it comprises Radix Notoginseng streptococcus acidi lactici fermented solution and a kind of pharmaceutically acceptable carrier or the excipient of effective dose.Wherein, cancer can be breast carcinoma, carcinoma of prostate, leukemia, rectal cancer, uterus carcinoma, ovarian cancer, carcinoma of endometrium, cervical cancer, carcinoma of testis, lymphatic cancer, rhabdomyosarcoma, neuroblastoma, pancreatic cancer, pulmonary carcinoma, brain tumor, skin carcinoma, gastric cancer, hepatocarcinoma, renal cancer or nasopharyngeal carcinoma, is preferably hepatocarcinoma.Medical composition of the present invention has the ability of anticancer growth, and suppression ratio reaches more than 60%, and medical composition of the present invention can be oral or injection type.
Medical composition of the present invention has the effect of the cancer of preventing and/or treating, and therefore can be administered to the cancer patient, accept chemotherapy or radiotherapy patient, cancer high-risk group etc.Compositions of the present invention has tight security, can not impact human body, can be for a long time as dietary supplement.
The invention provides a kind of Radix Notoginseng lactic fermentation liquid and preparation method thereof, Radix Notoginseng streptococcus acidi lactici fermented solution of the present invention has the ability of anticancer, hepatitis B surface antigen (HBsAg), and less to Normocellular toxicity.In addition, also can additionally add other material, increasing the biological activity of tunning of the present invention, as oxidation resistance etc.
[embodiment]
1, the preparation of Radix Notoginseng culture fluid
After Radix Notoginseng ground, obtain the Radix Notoginseng powder, the 10g Notoginseng Root is added in the 90ml water,, obtain the Radix Notoginseng culture fluid with high temperature sterilize still sterilization 20 minutes with 120 screen clothes.
2, with the lactic acid bacteria fermentation Radix Notoginseng
With lactobacillus inoculum to the Radix Notoginseng culture fluid of embodiment 1, lactobacillus is respectively streptococcus salivarius thermophilus (Streptococcus salivarius subsp.thermophilus (BCRC12268)), Switzerland's lactobacillus (Lactobacillus helveticus (BCRC14092)), thunder Man lactobacillus (Lactobacillus rhamnosusGG. (BCRC16000)), lactobacillus acidoilus (Lactobacillus acidophilus (BCRC10695)), luxuriant and rich with fragrance De Shi-Long root fungus (Bifidobacterium longum (BCRC14602)), chain bacillus bifidus (Bifidobacteriumcatenulatum (BCRC14667)), short in non-moral Salmonella (Bifidobacterium breve (BCRC11846)), initial bacterium number is 104cfu/ml, after cultivating 24 or 120 hours under 37 ℃, with the high temperature sterilize stuck fermentation, centrifugal filtration thalline and carbon nitrogen source solid content, obtain tunning, and test of the influence of the tunning of 1000 μ g/ml Hep 3B and Hep G2 cell.Organize in contrast with unleavened Radix Notoginseng culture fluid.Please refer to Fig. 1 a-1b, all Radix Notoginseng lactic fermenting products all have the ability that suppresses Hep 3B or Hep G2 cell, and are wherein preferable with the effect of Switzerland's lactobacillus (Lactobacillus helveticus).
3, the contrast of single lactobacillus and mixed vaccine
The fermentation flow process of present embodiment is similar to Example 2, but increase two fork lactobacilluss (Bifidobacteriumbifidum (BCRC14615)) and 8 kinds of mixing lactic acid bacteria fermentations, and test of the influence of the tunning of 200 μ g/ml Hep 3B and Hep G2 cell.Please refer to Fig. 2 a-2b, the more single lactobacillus height of the inhibition ability of mixing lactic acid bacteria.
4, fermentation temperature is to the influence of anticancer
The fermentation flow process of present embodiment is similar to Example 2, only changes fermentation temperature.Fermentation temperature is respectively 32 ℃, 37 ℃, 42 ℃, and the tunning (100 μ g/ml, 200 μ g/ml, 300 μ g/ml) of test variable concentrations is to the influence of Hep 3B and Hep 2G cell.Please refer to Fig. 3 a-3b, when fermentation temperature was 32 ℃, the fermented product that fermented 2 days had optimum efficiency; Please refer to Fig. 3 c-3d, when fermentation temperature was 37 ℃, the fermented product that fermented 3 days had optimum efficiency; Please refer to Fig. 3 e-3f, when fermentation temperature was 42 ℃, the fermented product that fermented 3 days had optimum efficiency.
5, pH-value is to the influence of anticancer
The fermentation flow process of present embodiment is identical with embodiment 2, the pH-value when only changing fermentation.The fermentation pH-value is respectively pH3.5, pH4.5, pH5.5, and the tunning (100 μ g/ml, 200 μ g/ml, 300 μ g/ml) of test variable concentrations is to the influence of Hep 3B and Hep 2G cell.Please refer to Fig. 4 a-4d, when pH value was 3.5 and 4.5, the fermented product that fermented 2 days had optimum efficiency.Please refer to Fig. 4 e-4f, when pH value was 5.5, fermented product did not then have any anticancer effect.
6, Semen sojae atricolor essence is got liquid to antioxidation effect
The fermentation flow process of present embodiment is identical with embodiment 2, only changes the composition of Radix Notoginseng culture fluid.Composition is respectively 1/4 Radix Notoginseng powder+3/4 Semen sojae atricolor powder, 1/2 Radix Notoginseng powder+1/2 Semen sojae atricolor powder, 3/4 Radix Notoginseng powder+1/4 Semen sojae atricolor powder, 4/4 Radix Notoginseng powder, and tests the influence of tunning to Hep 3B and Hep 2G cell, and the oxidation resistance of vitamin E is set at 100.Please refer to shown in Figure 5ly, fermented product antioxidative ability increases along with the increase of Semen sojae atricolor powder.
7, heat treatment Radix Notoginseng culture fluid is to the influence of anticancer
The fermentation flow process of present embodiment is identical with embodiment 2, only in advance the Radix Notoginseng culture fluid is heated before fermentation.With 121 ℃ of heating 20,60,120,180 minutes, test the influence of tunning respectively to Hep 3B and Hep 2G cell, and not heat as matched group.Please refer to shown in Fig. 6 a and the 6b, heating can increase the anticancer effect of fermented product.
8, Radix Notoginseng fermented solution is to normal hepatocellular influence
The fermentation flow process of present embodiment is identical with embodiment 2, and the fermentation natural law is 1 day.Handle the first of mice with tunning for hepatocyte (primary hepatocyte), and with MTT assay cell survival rate.Please refer to Fig. 7, if when the concentration of fermented product is 200 μ g/ml, just hepatocellular survival rate is more than 80% generation, and LD
50Be 976 μ g/ml.
9, Radix Notoginseng fermented solution is to the influence of hepatitis B surface antigen
The fermentation flow process of present embodiment is identical with embodiment 2, gets 1,3,5,7 day Radix Notoginseng fermented solution of fermentation respectively and handles Hep 3B cell, and measure the inhibition effect of tunning to the hepatitis B surface antigen of Hep 3B.Please refer to Fig. 8, suppressing effect increases along with the increase of fermentation time, and the Radix Notoginseng fermented solution that fermented 7 days has the suppression ratio more than 80%.
10, zoopery
Get the SCID mice of 18-22 gram, be divided into 5 groups, 10 every group.At first, inoculation Hep 3B/T2 cancerous cell is followed the Radix Notoginseng fermented product of feeding various dose in the underarm region of mice, continuous 35 days, measured a tumor size, and after stopping feeding, measure heart, liver, spleen, lungs, kidney and the tumor weight of mice respectively in every 3-4 days.
The Radix Notoginseng fermented product preparation condition of this embodiment is: the distilled water of the Radix Notoginseng powder and 90% (w/w) of 10% (w/w), and with above-mentioned 8 kinds of mixed vaccine, 37 ℃ of bottom fermentations 48 hours.The dosage of Radix Notoginseng fermented product is respectively 1000 μ g/ml (high dose group), 400 μ g/ml (middle dosage group) and 200 μ g/ml (low dose group), the positive control group is lumbar injection 5-FU, the negative control group is the feeding phosphate buffer, and matched group is not for inoculating Hep 3B/T2 cell.
Please refer to Fig. 9, the tumor minimum of positive matched group (5-FU) is high dose group (1000 μ g/ml) secondly, and the Radix Notoginseng fermented product of expression high dose has the effect that delays the tumor generation.In addition, the tumor of positive matched group (5-FU) is the lightest, is high dose group (1000 μ g/ml) secondly, and detailed results is as shown in table 1.
The weight of the heart of table 1 mice, liver, spleen, lungs, kidney and tumor (mg)
| Matched group | The negative control group | Low dose group | Middle dosage group | High dose group | The positive control group | |
| Heart | 149.0±19.7 | 116.8±17.8 | 111.8±9.5 | 107.9±5.5 | 122.7±11.9 | 143.2±14.6 |
| Liver | 1355±123.4 | 1114.5±143.4 | 1160.5±130.6 | 1211.5±130.6 | 1110.6±65.7 | 1171.3±103.0 |
| Spleen | 100.2±12.4 | 59.1±11.7 | 60.4±3.3 | 60.4±11.7 | 66.3±8.3 | 118.7±35.6 |
| Lungs | 190.6±8.4 | 178.8±14.2 | 178.5±31.2 | 185.3±13.6 | 191.9±19.5 | 215.8±23 |
| Kidney | 411.4±13.3 | 277.5±45 | 321.3±45.5 | 325.2±45.3 | 282.2±22.6 | 328.2±26.2 |
| Tumor | 1316.2±403.3 | 1169.9±310.9 | 1084.2±344.3 | 537.2±391.9 | 376.1±232.9 |
Though the present invention discloses as above in conjunction with the specific embodiment and accompanying drawing, it is not in order to limit the present invention.Any those skilled in the art without departing from the spirit and scope of the present invention, can modify or change the present invention.Therefore, protection scope of the present invention is as the criterion when defining with claim.
Claims (21)
1. the method for the Radix Notoginseng of fermenting, it comprises: with culture medium activation lactobacillus; Lactobacillus inoculum is fermented to the Radix Notoginseng culture fluid, to obtain fermentation liquid; Centrifugal fermentation liquid is obtained supernatant.
2. the method for fermentation Radix Notoginseng according to claim 1 is characterized in that: described method further comprises adds the Semen sojae atricolor extract in described Radix Notoginseng culture fluid.
3. the method for fermentation Radix Notoginseng according to claim 1, it is characterized in that: described method further comprises the described Radix Notoginseng culture fluid of heat treatment in advance.
4. the method for fermentation Radix Notoginseng according to claim 3, it is characterized in that: described heat treatment temperature is more than 75 ℃.
5. the method for fermentation Radix Notoginseng according to claim 3, it is characterized in that: described heat treatment time is more than 10 minutes.
6. the method for fermentation Radix Notoginseng according to claim 1, it is characterized in that: described lactobacillus is streptococcus salivarius thermophilus (Streptococcus salivarius subsp.Thermophilus (BCRC12268)), Switzerland's lactobacillus (Lactobacillus helveticus (BCRC14092)), thunder Man lactobacillus (Lactobacillusrhamnosus GG.(BCRC16000)), lactobacillus acidoilus (Lactobacillus acidophilus (BCRC10695)), luxuriant and rich with fragrance De Shi-Long root fungus (Bifidobacterium longum (BCRC14602)), chain bacillus bifidus (Bifidobacterium catenulatum (BCRC14667)), short than non-moral Salmonella (Bifidobacteriumbreve (BCRC 11846)), two fork lactobacillus (Bifidobacterium bifidum (BCRC14615)) or its combination.
7. the method for fermentation Radix Notoginseng according to claim 1, it is characterized in that: described culture medium is MRS or digestible protein-yeast extract (peptone-yeast extract base) culture medium.
8. the method for fermentation Radix Notoginseng according to claim 1, it is characterized in that: described Radix Notoginseng culture fluid comprises the Notoginseng Root of 10-15% (w/w) and the water of 85-90% (w/w).
9. the method for fermentation Radix Notoginseng according to claim 1, it is characterized in that: described fermentation time is 1 to 7 day.
10. the method for fermentation Radix Notoginseng according to claim 1, it is characterized in that: the temperature of described fermentation is at 25 to 40 ℃.
11. the method for fermentation Radix Notoginseng according to claim 1, it is characterized in that: the pH value of described fermentation is between 3 to 5.
12. a Radix Notoginseng streptococcus acidi lactici fermented solution by the described method preparation of claim 1, has the ability of anticancer growth.
13. Radix Notoginseng streptococcus acidi lactici fermented solution according to claim 12 is characterized in that: described Radix Notoginseng streptococcus acidi lactici fermented solution has the ability that suppresses the hepatitis B surface antigen.
14. Radix Notoginseng streptococcus acidi lactici fermented solution according to claim 12 is characterized in that: described cancerous cell comprises breast cancer cell, prostate gland cancer cell, blood cell, rectum cancer cell, uterus carcinoma cell, ovarian cancer cell, endometrial carcinoma cell, cervical cancer cell, testicular cancer cell, lymphocytic cancer cell, human rhabdomyosarcoma cells, neuroblastoma cell, pancreatic cancer cell, lung carcinoma cell, brain tumor cell, skin cancer cell, stomach cancer cell, hepatoma carcinoma cell, renal cancer cell or nasopharyngeal carcinoma cell.
15. Radix Notoginseng streptococcus acidi lactici fermented solution according to claim 12 is characterized in that: described cancerous cell is a hepatoma carcinoma cell.
16. Radix Notoginseng streptococcus acidi lactici fermented solution according to claim 12 is characterized in that: described Radix Notoginseng streptococcus acidi lactici fermented solution has the anticancer effect more than 60%.
17. a medical composition that prevents and/or treats cancer, it comprises:
The Radix Notoginseng streptococcus acidi lactici fermented solution of effective dose is by the described method preparation of claim 1; And
A kind of pharmaceutically acceptable carrier or excipient.
18. the medical composition that prevents and/or treats cancer according to claim 17 is characterized in that: described cancer comprises breast carcinoma, carcinoma of prostate, leukemia, rectal cancer, uterus carcinoma, ovarian cancer, carcinoma of endometrium, cervical cancer, carcinoma of testis, lymphatic cancer, rhabdomyosarcoma, neuroblastoma, pancreatic cancer, pulmonary carcinoma, brain tumor, skin carcinoma, gastric cancer, hepatocarcinoma, renal cancer or nasopharyngeal carcinoma.
19. the medical composition that prevents and/or treats cancer according to claim 17 is characterized in that: described cancer is a hepatocarcinoma.
20. according to the claim the 17 described medical compositions that prevent and/or treat cancer, it is characterized in that: described medical composition has the anticancer effect more than 60%.
21. the medical composition that prevents and/or treats cancer according to claim 17 is characterized in that: described medical composition is oral or injection type.
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| CN102696819A (en) * | 2011-05-11 | 2012-10-03 | 谢永林 | Preparation method of sanqi (panax notoginseng) tea |
| CN103520216A (en) * | 2013-04-08 | 2014-01-22 | 柳志信 | Probiotic human injection |
| CN104825383A (en) * | 2015-05-28 | 2015-08-12 | 云南白药集团股份有限公司 | Pseudo-ginseng yeast extraction method and application |
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| CN102696819A (en) * | 2011-05-11 | 2012-10-03 | 谢永林 | Preparation method of sanqi (panax notoginseng) tea |
| CN103520216A (en) * | 2013-04-08 | 2014-01-22 | 柳志信 | Probiotic human injection |
| CN104825383A (en) * | 2015-05-28 | 2015-08-12 | 云南白药集团股份有限公司 | Pseudo-ginseng yeast extraction method and application |
| CN108434222A (en) * | 2018-06-12 | 2018-08-24 | 黄淮学院 | A kind of compound Chinese medicinal preparation that treating ovarian neoplasm and its application |
| CN108434222B (en) * | 2018-06-12 | 2020-12-18 | 黄淮学院 | Traditional Chinese medicine compound preparation for treating ovarian tumor and application thereof |
| CN112608949A (en) * | 2020-12-18 | 2021-04-06 | 西南林业大学 | Preparation method and application of pseudo-ginseng flower extract |
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