CN101269095A - Supercritical combined processing method of ganoderma lucidum and ganoderma lucidum spore - Google Patents
Supercritical combined processing method of ganoderma lucidum and ganoderma lucidum spore Download PDFInfo
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- CN101269095A CN101269095A CNA2008100238349A CN200810023834A CN101269095A CN 101269095 A CN101269095 A CN 101269095A CN A2008100238349 A CNA2008100238349 A CN A2008100238349A CN 200810023834 A CN200810023834 A CN 200810023834A CN 101269095 A CN101269095 A CN 101269095A
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Abstract
The invention relates to a supercritical composite processing method of ganoderma-ganoderma spore, which adopts an advanced supercritical CO2 fluid chemical separation technique and prepares ganoderma fruit powder and broken ganoderma spore powder into a composite powder. Inert gas CO2 is utilized for an extraction in a supercritical state and simultaneously, entrainer water of 30 to 50 percent and ethanol are infiltrated to form a composite processing method. The supercritical composite processing method of ganoderma-ganoderma spore has simple and effective process and the composite powder relates to cost reduction and quality improvement and is favorable for the smoothness and safety of the CO2 fluid during the extraction. The introduction of the entrainer is beneficial for extracting water-soluble and fat-soluble substances in ganoderma and obtaining a supercritical ganoderma spore oil of high enrichment, high grade and high efficacy.
Description
Technical field
The present invention relates to a kind of novel advanced chemical separation technology, specifically Ganoderma and sporoderm-broken Ganoderma spore are made a kind of composite powder, in a kind of extraction process that utilizes supercritical fluid, penetrating agent such as continuous water leaking-in, ethanol, a kind of combined processing method that extracts the Ganoderma spore oil of high-purity, high concentration, high effect.
Summary of the invention
Ganoderma is as health care, and it is with a long history in China to cure the disease, ancient times, people liken Ganoderma to " Herba mesonae chinensis ", " elixir ", the record of Li Shizhen (1518-1593 A.D.) Compendium of Material Medica, Ganoderma " Gan Wen, nontoxic; cure mainly deafness; sharp joint protects kidney, beneficial vital essence, hard muscles and bones; good color; clothes are made light of one's life by commiting suicide for a long time, and are not old, prolong life ".Ganoderma is the Chinese medicine treasure.
Modern times have been carried out the pharmacology and the medicine research of system to Ganoderma both at home and abroad, and the confirmation Ganoderma can be improved body vitality and resistance against diseases, promotes the damaging cells rehabilitation, suppress tumor growth, the human body immunity improving function is detoxified, protects the liver, is calmed the nerves, and improves superoxide dismutase content etc.Cooperating other drug to use has potentiation, and body is had no adverse reaction.
Studies confirm that simultaneously the effective ingredient that Ganoderma spore is contained and the composition of Ganoderma sporophore are basic identical, but Ganodenic acid institute content does not have the Ganoderma entity abundant like that, the price of opposite Ganoderma spore powder with cellular wall broken exceeds the price of Ganoderma sporophore far away.Both Chinese medicine ingredients have ganoderan, Ganodenic acid, adenosine, and phytosterin compound generates alkali etc.
From Ganoderma or Ganoderma spore effective component extracting processing method, two kinds of modes commonly used: (1) ethanol extract from water precipitation (2) decoction and alcohol sedimentation technique.The former uses ethanol extraction earlier, and aqueous precipitation is made Ganoderma extractum in extracting solution, and the latter uses earlier water extraction, and the back adds ethanol precipitation, makes Ganoderma extractum.These two kinds of traditional methods, complex process, cost is higher relatively, extraction temperature height has some effective active compositions in the Ganoderma, can sustain damage and destroy, to use a large amount of chemical reagent in the extraction process, easily remain in the Ganoderma extractum, influence product quality and medical effect, the contaminated environment that two kinds of extracting method can be to a certain degree.
Domestic also useful supercritical extracts Ganoderma spore oil from Ganoderma spore now, is a much progress on the extractive technique.But it is because extremely trickle through the spore powder with crushed sporoderm of superfine grinding, under supercriticality, powerful air pressure very easily makes spore powder stop up extracting system and causes safety problem, therefore general the employing increases complicated pre-treating technology, the Ganoderma spore powder water is added gelatin, binding agents such as starch are modulated the extrusion modling of heating again earlier, and dry back is standby.This method though can prevent to stop up, has also influenced fluid and the tight of spore powder and has contacted, thereby influences the concentration of row yielding and spore oil effective ingredient.
Summary of the invention
The objective of the invention is to seek the supercritical combined processing method of a kind of Ganoderma and sporoderm-broken Ganoderma spore, contact, improve the concentration of row yielding and spore oil effective ingredient to impel fluid and the tight of spore powder.
According to technical scheme provided by the invention, earlier Ganoderma sporophore is ground into 80～100 purpose REISHs, with the synthetic Ganoderma of Sporoderm-broken Ganoderma Lucidum Spore powder-Ganoderma spore composite powder, described composite powder ratio is: Ganoderma powder 30%～70%, Sporoderm-broken Ganoderma Lucidum Spore powder 70%～30% then; Then above-mentioned composite powder is packed into and utilize CO in the extraction kettle
2Fluid extracts, and constantly infiltrates entrainer when extraction, forms compound fluid; The addition of described entrainer is 5%～50% of a composite powder, 2～24 hours extraction time, extracting pressure 20～45mpa, 35 ℃～55 ℃ of extraction temperature.
After extraction, compound fluid is decompressed to send in the separating still behind 3～10mpa separates, to extract the effective ingredient in the composite powder.The compound fluid that will be decompressed to once more behind 3～10mpa is sent in the separating still, carries out secondary separation, with the effective ingredient in the further extraction composite powder.
Configurating filtered net or filter bag in the gas outlet of extraction kettle in order to avoid stop up the gas outlet, guarantee CO
2Fluid is unimpeded.Described entrainer is water, ethanol, ethyl acetate or glycerol.In described composite powder, also comprise propolis powder, Fructus Perillae powder, linseed meal, one or more in the Fructus Momordicae charantiae powder.Described effective ingredient comprises Ganoderma oil.
Innovation of the present invention and advantage: with traditional supercritical CO of using
2The single extracting process of the Ganoderma spore that fluid generally adopts is modified into the compound extraction of composite powder, and advantage is that the effective ingredient that will be dissolved in water and the ethanol also can extract.
The present invention is better than traditional diamond-making technique, also is better than existing supercritical processing method, and advantage is as follows: 1, simplified pretreatment procedure, do not needed traditional complicated procedures of forming of heat drying again that Ganoderma spore added after additive, the extrusion modling; 2, once can extract the effective active composition of Ganoderma object more than two kinds; 3, improve yield rate; 4, improve the active constituent content of health-care hospital in the product; 5, improve comprehensive utilization ratio; 6, reduce environmental pollution; 7, can obtain high concentration, high effect, high Ganodenic acid, high-quality Ganoderma spore oil; 8, reduce raw material and processing cost.
Comprehensive above advantage, the favourable lifting Chinese medicine of processing method of the present invention treasure Ganoderma is in the competitiveness of international market.
Description of drawings
Fig. 1 is a process chart of the present invention.
The specific embodiment
1, Ganoderma sporophore is ground into bigger powdered granule and the ultra-fine Ganoderma spore powder of breaking cellular wall in 80～100 staring blanklies footpath, is uniformly mixed into Ganoderma-Ganoderma spore composite powder;
2, with Ganoderma after compound and Ganoderma spore composite powder, be incorporated with in the extraction kettle of special fine and closely woven metal screen and worry plate, because the support of bulky grain Ganoderma powder and filter screen add, guaranteed not stop up CO
2Fluidic unimpeded and safety, extraction smoothly.35 ℃～55 ℃ of extraction temperature, extracting pressure 20～45mpa, 2～24 hours extraction time.
3, in extraction kettle, CO
2When fluid extracts, constantly with water or ethanol as penetrating agent, inject extraction kettle and carry out the combined type extraction.
4, in the extraction kettle, be dissolved in CO
2The Ganoderma in the fluid and the effective ingredient such as Ganodenic acid of Ganoderma spore, and CO
2Fluid enters separating still, first separating pressure, 4～10mpa, second separating pressure, 1.5～4mpa, CO through the air relief valve decompression together
2After fluid loses supercriticality, separate out compound supercritical Ganoderma Lucidum spore oil.
5, after the extraction, remain in Ganoderma and Ganoderma spore composite powder in the extraction kettle, also have certain medical value, can fully utilize.
Embodiment 1, with the clean Ganoderma sporophore in selected back, is crushed to about fineness 80～100 orders with pulverizer, selects sporoderm-broken rate, reaches the Ganoderma spore powder more than 75～95%, with the two Ganoderma powder 40% Ganoderma spore powder 60% in proportion.Stirring is made uniform Ganoderma-Ganoderma spore composite powder weighing and is packed in the extraction kettle, utilizes CO
2Gas extracts in extraction kettle.CO
2Gas is at 31.1 ℃, and pressure is at 75.2kg/cm
2The time, just be in critical state.CO
2After gas enters supercriticality, increase in density, solvability strengthens, and colourless, tasteless, nontoxic is ideal solvent.
This example extracting pressure 45mpa, 55 ℃ of extraction temperature are at CO
2In the time of fluid extraction, constantly infiltrate entrainer water, 75%～95% ethanol, infiltration capacity is 30% of a composite powder, in Ganoderma-Ganoderma spore, and the polysaccharide in the water-soluble and ethanol, fat-soluble a lot of effective ingredient extracts simultaneously, 8 hours extraction time, the supercritical CO after the extraction
2Fluid, decompression enters first separating still, and separating pressure 10mpa enters second separating still again, separating pressure 3mpa, extraction finishes to obtain required supercritical Ganoderma-Ganoderma spore extract respectively at two separating stills.
Embodiment 2, Ganoderma powder 50% and Ganoderma spore powder 50%, extracting pressure 40mpa, 50 ℃ of extraction temperature, entrainer infiltration capacity 25%, 10 hours extraction time, the first separating pressure 10mpa, the second separating pressure 3mpa.
Embodiment 3, Ganoderma powder 40% and Ganoderma spore powder 60%, extracting pressure 35mpa, 45 ℃ of extraction temperature, entrainer infiltration capacity 20%, 14 hours extraction time, the first separating pressure 10mpa, the second separating pressure 3mpa.
Embodiment 4, Ganoderma powder 45% and Ganoderma spore powder 55%, extracting pressure 30mpa, 40 ℃ of extraction temperature, entrainer infiltration capacity 15%, 18 hours extraction time, the first separating pressure 10mpa, the second separating pressure 3mpa.
Embodiment 5, Ganoderma powder 55% and Ganoderma spore powder 45%, extracting pressure 25mpa, 35 ℃ of extraction temperature, entrainer infiltration capacity 10%, 21 hours extraction time, the first separating pressure 10mpa, the second separating pressure 3mpa.
Embodiment 6, Ganoderma powder 60% and Ganoderma spore powder 40%, extracting pressure 20mpa, 35 ℃ of extraction temperature, entrainer infiltration capacity 8%, 24 hours extraction time, the first separating pressure 10mpa, the second separating pressure 3mpa.
The present invention makes supercritical CO
2The method of extracting ganoderma spore obtains further improving and improving, and has more practicality and science, and reducing cost has promoted product quality and effect.
1, the main effective component Ganoderma terpenoid that is rich in the Ganoderma spore is liposoluble substance, and is water insoluble, is dissolved in ethanol, CO
2Under supercriticality, the suitable liposoluble substance that extracts is aided with ethanol again and infiltrates, and can improve 8%～10% manufactured goods.
2, polyoses content is less in the Ganoderma spore, and ganoderan is a water-soluble substances, and is water-soluble and be insoluble to ethanol, also is difficult for by supercritical CO
2Extract, the present invention is auxiliary with the method for water leaking-in, has increased ganoderan component content in the spore oil.
3, scientific experiments proves, the composition that active ingredient that Ganoderma spore contained and Ganoderma sporophore are contained is basic identical, and containing more ganoderan in the Ganoderma sporophore is the effective constituent that mainly contains of Ganoderma.Have and strengthen and the body vitality, the human body immunity improving function, defying age, life-saving suppresses effects such as tumor growth, the present invention adopts the composite powder of Ganoderma spore and Ganoderma sporophore, has increased the favourable lifting product quality of polysaccharide composition and the effect of Ganoderma spore oil.
4, the employing of Ganoderma sporophore can reduce product cost greatly, because the price of Ganoderma spore powder is approximately higher than Ganoderma sporophore more than ten times.
5, technology is simple, and easy operating does not need complicated pretreatment procedure, and is pollution-free, do not have discharging, the favorable environment protection.
The present invention is compared as follows with original method
Claims (7)
1. Ganoderma, Ganoderma spore supercritical combined processing method, it is characterized in that: earlier Ganoderma sporophore is ground into 80～100 purpose REISHs, then with the synthetic Ganoderma of Sporoderm-broken Ganoderma Lucidum Spore powder-Ganoderma spore composite powder, described composite powder ratio is: Ganoderma powder 30%～70%, Sporoderm-broken Ganoderma Lucidum Spore powder 70%～30%; Then above-mentioned composite powder is packed into and utilize CO in the extraction kettle
2Fluid extracts, and constantly infiltrates entrainer when extraction, forms compound fluid; The addition of described entrainer is 5%～30% of a composite powder, 2～24 hours extraction time, extracting pressure 20～45mpa, 35 ℃～55 ℃ of extraction temperature.
2. according to claims 1 described method, it is characterized in that: after extraction, compound fluid is decompressed to send in the separating still behind 3～10mpa separates, to extract the effective ingredient in the composite powder.
3. according to claims 2 described methods, it is characterized in that: the compound fluid that will be decompressed to once more behind 3～10mpa is sent in the separating still, carries out secondary separation, with the effective ingredient in the further extraction composite powder.
4. according to right 1 described method, it is characterized in that: configurating filtered net or filter bag in the gas outlet of extraction kettle in order to avoid stop up the gas outlet, guarantee CO
2Fluid is unimpeded.
5. according to right 1 described method, it is characterized in that: described entrainer is water, ethanol, ethyl acetate or glycerol.
6. according to right 1 described method, it is characterized in that: in described composite powder, also comprise propolis powder, Fructus Perillae powder, linseed meal, one or more in the Fructus Momordicae charantiae powder.
7. according to right 1 described method, it is characterized in that: described effective ingredient comprises Ganoderma oil.
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831351A (en) * | 2010-06-03 | 2010-09-15 | 吉林大学 | Method for extracting ganoderma oil with supercritical carbon dioxide fluid |
| CN101933540A (en) * | 2010-09-17 | 2011-01-05 | 香格里拉县圣宝食品进出口有限责任公司 | Edible wild fungus oil |
| CN103652837A (en) * | 2013-12-03 | 2014-03-26 | 湖北省农业科学院农产品加工与核农技术研究所 | Cell wall broken Ganoderma Lucidum spore powder-Perilla herb oil composite softgel and preparation technology thereof |
| CN104274498A (en) * | 2014-09-18 | 2015-01-14 | 开平健之源保健食品有限公司 | Efficient antineoplastic lucid ganoderma extract and preparation method thereof |
| CN105754714A (en) * | 2016-03-10 | 2016-07-13 | 广东粤微食用菌技术有限公司 | Method for extracting ganoderma spore oil without granulation supercritical CO2 |
| CN113150867A (en) * | 2020-12-01 | 2021-07-23 | 中科健康产业集团股份有限公司 | Preparation method of ganoderma lucidum extract oil rich in ganoderma lucidum triterpenes |
| CN114736259A (en) * | 2022-03-03 | 2022-07-12 | 苏州猫尔科技有限公司 | Supercritical CO2Method for extracting ganoderma lucidum adenosine |
| CN115006874A (en) * | 2022-06-14 | 2022-09-06 | 中科健康产业集团股份有限公司 | Extraction process of ganoderma lucidum and ganoderma lucidum spores rich in ganoderic acid components |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1094766C (en) * | 1999-11-19 | 2002-11-27 | 中国科学院南京地理与湖泊研究所科技开发公司 | Supercritical processing method of ganoderma spore |
-
2008
- 2008-04-17 CN CN2008100238349A patent/CN101269095B/en not_active Expired - Fee Related
Cited By (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101831351A (en) * | 2010-06-03 | 2010-09-15 | 吉林大学 | Method for extracting ganoderma oil with supercritical carbon dioxide fluid |
| CN101933540A (en) * | 2010-09-17 | 2011-01-05 | 香格里拉县圣宝食品进出口有限责任公司 | Edible wild fungus oil |
| CN103652837A (en) * | 2013-12-03 | 2014-03-26 | 湖北省农业科学院农产品加工与核农技术研究所 | Cell wall broken Ganoderma Lucidum spore powder-Perilla herb oil composite softgel and preparation technology thereof |
| CN104274498A (en) * | 2014-09-18 | 2015-01-14 | 开平健之源保健食品有限公司 | Efficient antineoplastic lucid ganoderma extract and preparation method thereof |
| CN104274498B (en) * | 2014-09-18 | 2018-05-15 | 开平健之源保健食品有限公司 | A kind of high efficiency anti-tumor Ganodenna Lucidum P.E and preparation method thereof |
| CN105754714A (en) * | 2016-03-10 | 2016-07-13 | 广东粤微食用菌技术有限公司 | Method for extracting ganoderma spore oil without granulation supercritical CO2 |
| CN113150867A (en) * | 2020-12-01 | 2021-07-23 | 中科健康产业集团股份有限公司 | Preparation method of ganoderma lucidum extract oil rich in ganoderma lucidum triterpenes |
| CN114736259A (en) * | 2022-03-03 | 2022-07-12 | 苏州猫尔科技有限公司 | Supercritical CO2Method for extracting ganoderma lucidum adenosine |
| CN114736259B (en) * | 2022-03-03 | 2023-11-10 | 苏州猫尔科技有限公司 | Supercritical CO 2 Method for extracting ganoderma lucidum adenosine |
| CN115006874A (en) * | 2022-06-14 | 2022-09-06 | 中科健康产业集团股份有限公司 | Extraction process of ganoderma lucidum and ganoderma lucidum spores rich in ganoderic acid components |
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