CN101264096A - 人参皂苷Rg1的生精作用 - Google Patents

人参皂苷Rg1的生精作用 Download PDF

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CN101264096A
CN101264096A CNA2007100643260A CN200710064326A CN101264096A CN 101264096 A CN101264096 A CN 101264096A CN A2007100643260 A CNA2007100643260 A CN A2007100643260A CN 200710064326 A CN200710064326 A CN 200710064326A CN 101264096 A CN101264096 A CN 101264096A
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sperm
gossypol
ginsenoside
group
motility
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CN101264096B (zh
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张均田
楚世峰
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JICUI HEALTH INDUSTRY CO., LTD.
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Institute of Materia Medica of CAMS
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Priority to EP07846012.8A priority patent/EP2133082B8/en
Priority to DK07846012.8T priority patent/DK2133082T3/da
Priority to JP2009552987A priority patent/JP2010520894A/ja
Priority to US12/530,970 priority patent/US8765695B2/en
Priority to PT78460128T priority patent/PT2133082E/pt
Priority to PL07846012T priority patent/PL2133082T3/pl
Priority to CA2680560A priority patent/CA2680560C/en
Priority to PCT/CN2007/003776 priority patent/WO2008110050A1/zh
Priority to ES07846012.8T priority patent/ES2496165T3/es
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    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7028—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages
    • A61K31/7034—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin
    • A61K31/704—Compounds having saccharide radicals attached to non-saccharide compounds by glycosidic linkages attached to a carbocyclic compound, e.g. phloridzin attached to a condensed carbocyclic ring system, e.g. sennosides, thiocolchicosides, escin, daunorubicin
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P15/00—Drugs for genital or sexual disorders; Contraceptives
    • A61P15/10—Drugs for genital or sexual disorders; Contraceptives for impotence

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Veterinary Medicine (AREA)
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  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Organic Chemistry (AREA)
  • Gynecology & Obstetrics (AREA)
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  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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Abstract

本发明公开了人参皂甙Rg1的制备生精产品的用途。本发明还公开了一种生精的产品，其包括人参皂甙Rg1单体及赋形剂或载体。

Description

人参皂苷Rg1的生精作用
技术领域
本发明涉及人参皂甙Rg1的制备生精产品的用途；本发明还涉及一种生精的产品，其包括人参皂甙Rg1单体及赋形剂或载体。
背景技术
据统计，全世界育龄夫妇中，不孕者约占15％，而不孕因素在男方约占40％，20世纪以来，由于环境污染，性病和艾滋病蔓延，过度吸烟吸毒，滥用激素类药物等因素，全球范围内的男性生育能力有明显下降的趋势，在男性不育症中关键因素之一是精子质量(精子数量和活力)的降低，，寻找改善改善精子质量的药物甚为重要。
本发明的发明人过去的研究已证明人参皂苷Rg1能增强雄性动物的性欲，性功能及阴茎勃起。
发明内容
为了解决现有技术中存在的技术问题，本发明提供了如下技术方案：
本发明提供了人参皂甙Rg1的制备生精产品的用途。
本发明还提供了一种生精的产品，其包括人参皂甙Rg1单体及赋形剂或载体。
本发明的发明人经过系统研究现发现Rg1有促进生精的作用。
已知含于棉子油中的棉酚是一种毒剂，它破坏睾丸组织的生精，致精子数量减少，存活率降低，精子活力下降。本发明的发明人给成年大鼠ip醋酸棉酚建立大鼠生精障碍模型。发明人发现大鼠在给棉酚的同时，每天以不同的剂量灌胃人参皂苷Rg1，能够Rg1显著改善棉酚所致的精子数量减少，存活率降低，精子活力下降，证明Rg1可以增加精子数量，提高精子存活率，增强精子活力。
本发明的发明人进一步研究了人参皂苷Rg1促生精作用的机制。棉酚破坏精子功能主要是干扰精子细胞的变态和中晚期精母细胞的发育，导致精子损伤和曲细精管的萎缩。研究发现棉酚对精子的破坏作用主要是由于它在睾丸组织中产生过量的一氧化氮(NO)，NO作为自由基，一方面破坏生精组织和精子细胞，一方面破坏间质细胞，使睾酮(T)的产生和释放降低。发明人用发射免疫法测定检测睾丸组织内一氧化氮(NO)含量，血清中睾酮(T)，促黄体生成素(LH)，和促卵泡生成素(FSH)。检测结果Rg1可显著降低睾丸组织内过量的NO，从而保护精子免受NO的破坏并升高血中T的含量。而Rg1对血中LH和FSH含量无显著影响(P＞0.05)。
具体实施方式
例1人参皂苷Rg1的促生精作用
成年雄性Wister大鼠，每组10只，共5组。1组为空白对照组，另外4组ip.醋酸棉酚50mg/kg，隔天一次，共七次。一组动物只给棉酚，另三组在给棉酚的同时，每天灌胃人参皂苷Rg1，剂量分别为5，10，15mg/kg，每天一次，共十四次。给药两周后处死动物，取出附睾尾，对精子数量，精子存活率何精子活力进行检测，检测结果如下：
表1人参皂苷Rg1对醋酸棉酚所致精子数量下降的影响
组别   附睾尾精子计数(106/ml)x±s P值
  模型组   110.82±28.59
  空白组(未给棉酚组)   139.27±29.27   ＜0.01
  棉酚+Rg15mg/kg   216.80±31.26   ＜0.01
  棉酚+Rg110mg/kg   256.61±53.73   ＜0.01
  棉酚+Rg115mg/kg   235.49±39.71   ＜0.01
表2人参皂苷Rg1对精子存活率的影响
  组别   精子活率(％)x±s   P值
  模型组   44.52±8.8
  空白组(未给棉酚组)   53.09±7.0   ＜0.05
  棉酚+Rg15mg/kg   44.12±4.79
  棉酚+Rg110mg/kg   62.64±9.94   ＜0.05
  棉酚+Rg115mg/kg   56.69±6.48   ＜0.05
表3人参皂苷Rg1对精子活力的影响
  组别  精子活力(a+b级)(％)x±s   P值
  模型组  29.08±5.97
  空白组(未给棉酚组)  35.58±6.08   ＜0.05
  棉酚+Rg15mg/kg  27.60±5.95
  棉酚+Rg110mg/kg  44.97±8.08   ＜0.01
  棉酚+Rg115mg/kg  36.16±6.55   ＜0.05
按WHO的标准，a级精子是指快速前向运动的精子；b级精子是指慢或呆滞的前向运动的精子；c级精子是指非前向运动的精子；d级精子是指不动者
表1-3的结果说明，醋酸棉酚50mg/kg口服7次可造成精子数量减少，精子存活率降低，精子活力下降，Rg1则可以增加精子数量，提高存活率，增强精子活力，具有明显的改善作用。
例2人参皂苷Rg1促生精作用的机制研究
同时用发射免疫法测定血清中睾酮(T)，促黄体生成素(LH)，和促卵泡生成素(FSH)，并采用南京建成NO试剂盒检测睾丸组织内一氧化氮(NO)含量，具体操作见说明书。
表4.人参皂苷Rg1对睾丸组织一氧化氮(NO)含量的影响
  组别   NO含量(μmol/gpro)x±s   P值
  模型组   20.09±9.42
  空白组(未给棉酚组)   9.48±6.21   ＜0.05
  棉酚+Rg1 5mg/kg   16.01±8.86
  棉酚+Rg1 10mg/kg   9.71±6.79   ＜0.05
  棉酚+Rg1 15mg/kg   10.78±4.58   ＜0.01
表5.人参皂苷Rg1对血清睾酮(T)含量的影响
  组别   T含量(ng/ml)x±s   P值
  模型组   0.28±0.17
  空白组(未给棉酚组)   1,18±1.01   ＜0.05
  棉酚+Rg15mg/kg   0.816±0.32   ＜0.05
  棉酚+Rg110mg/kg   0.97±0.48   ＜0.05
  棉酚+Rg115mg/kg   0.82±0.24   ＜0.01
Rg1可显著降低睾丸组织内过量的NO，从而保护精子免受NO的破坏见表4；升高血中T的含量见。
而Rg1对血中LH和FSH含量无显著影响(P＞0.05)。

Claims (2)

1. 人参皂甙Rg1在制备生精产品中用途。
2. 一种生精的产品，其包括人参皂甙Rg1单体及赋形剂或载体。
CN2007100643260A 2007-03-12 2007-03-12 人参皂苷Rg1在制备生精产品中的应用 Active CN101264096B (zh)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CN2007100643260A CN101264096B (zh) 2007-03-12 2007-03-12 人参皂苷Rg1在制备生精产品中的应用
PCT/CN2007/003776 WO2008110050A1 (fr) 2007-03-12 2007-12-25 UTILISATION DE GINSENOSIDE Rg1 ET DE SES MÉTABOLITES GINSENOSIDE Rh1 ET PpT
JP2009552987A JP2010520894A (ja) 2007-03-12 2007-12-25 ジンセノサイドＲｇ１、その代謝産物であるジンセノサイドＲｈ１および／またはＰｐｔの使用
US12/530,970 US8765695B2 (en) 2007-03-12 2007-12-25 Use of ginsenoside RG1, its metabolites ginsenoside RH1 and/or PPT
PT78460128T PT2133082E (pt) 2007-03-12 2007-12-25 Utilização do ginsenósido rg1 e dos seus metabolitos ginsenósidos rh1 e ppt
PL07846012T PL2133082T3 (pl) 2007-03-12 2007-12-25 Stosowanie ginsenozydu Rg1, jego metabolitów ginsenozydu Rh1 i/lub Ppt
EP07846012.8A EP2133082B8 (en) 2007-03-12 2007-12-25 THE USE OF GINSENOSIDE Rg1, ITS METABOLITES GINSENOSIDE Rh1 AND/OR PpT
DK07846012.8T DK2133082T3 (da) 2007-03-12 2007-12-25 Anvendelse af ginsenosid Rg1, dets metabolitter ginsenosid Rh1 og/eller PpT
ES07846012.8T ES2496165T3 (es) 2007-03-12 2007-12-25 El uso del ginsenósido Rg1 y sus metabolitos los ginsenósidos Rh1 y/o PpT
CA2680560A CA2680560C (en) 2007-03-12 2007-12-25 The use of ginsenoside rg1, its metabolites ginsenoside rh1 and/or ppt
JP2013169853A JP2013256519A (ja) 2007-03-12 2013-08-19 ジンセノサイドＲｇ１、その代謝産物であるジンセノサイドＲｈ１および／またはＰｐｔの使用

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ES (1) ES2496165T3 (zh)
PL (1) PL2133082T3 (zh)
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CN112772630A (zh) * 2020-12-30 2021-05-11 西南大学 一种施氏鲟精子低温保存稀释液及其制备方法

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CN102000098B (zh) * 2004-08-11 2015-08-12 中国医学科学院药物研究所 人参皂甙Rg1的用途
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Publication number Priority date Publication date Assignee Title
CN117643592A (zh) * 2023-11-27 2024-03-05 永创恒新生物医学科技(北京)有限公司 人参皂苷20(R)-25-OH-Rg2促进卵巢修复的用途
CN117643592B (zh) * 2023-11-27 2024-05-28 四川花度蜜美医学研究院 人参皂苷20(R)-25-OH-Rg2促进卵巢修复的用途

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CA2680560A1 (en) 2008-09-18
CA2680560C (en) 2015-11-24
JP2010520894A (ja) 2010-06-17
EP2133082A4 (en) 2010-03-24
EP2133082B1 (en) 2014-06-11
EP2133082A1 (en) 2009-12-16
CN101264096B (zh) 2011-11-09
ES2496165T3 (es) 2014-09-18
JP2013256519A (ja) 2013-12-26
EP2133082B8 (en) 2014-07-16
PT2133082E (pt) 2014-09-04
DK2133082T3 (da) 2014-08-25
WO2008110050A1 (fr) 2008-09-18
PL2133082T3 (pl) 2014-11-28
US8765695B2 (en) 2014-07-01
US20110034403A1 (en) 2011-02-10

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