CN101255228A

CN101255228A - Method for synthesizing polyhydroxyalkanoates by employing organic sewage water

Info

Publication number: CN101255228A
Application number: CNA2008100527207A
Authority: CN
Inventors: 邵胜学; 崔秋凯
Original assignee: Individual
Current assignee: Individual
Priority date: 2008-04-14
Filing date: 2008-04-14
Publication date: 2008-09-03

Abstract

The invention discloses a method for synthesizing polyhydroxyalkanoate using organic sewage, comprising depositing and collecting microbe during treating the organic sewage and conveying the microbe into a container for extracting polyhydroxyalkanoate; adjusting the pH at 4-12 and adding petroleum ether or chloroform, tetrachloromethane or solvent oil in proportion of 1:1 and intensively mixing the solvent at 10-80 degree; extracting organic phase after layering; laying the extracted organic phase in an airtight drying apparatus to dry and recovery the solvent; finally obtaining dry product, namely polyhydroxyalkanoate. The organic sewage is treated by the method to obtain polyhydroxyalkanoate and it is needless to subject specially cultivating and domesticating to the microbe concerned with the treatment of the organic sewage, the microbe can be obtained by general method for culturing microbe and it is unnecessary to add the other microbe during production process. The method has low cost and the operation is simple.

Description

Utilize the method for organic sewage synthesizing polyhydroxyalkanoateby

Technical field

The invention relates to the method for utilizing the organic sewage synthesizing polyhydroxyalkanoateby.

Background technology

In various forms of environmental pollutions, water pollution is the most deep to human lives's influence, and therefore, in the improvement of environmental pollution, sewage disposal also just receives much attention naturally.Nearly three in a decade or so, the sewage disposal technology of China not only is widely used, and obtained swift and violent development, for China suppresses environmental pollution, improve the people's quality of life and made huge contribution, but, we can not deny, the application of our sewage disposal technology, all be to realize to sacrifice huge economic profit, and a large amount of refuses that sewage treatment process produces have become our difficult problem in the process of curbing environmental pollution again, especially a large amount of excess sludges of organic sewage treating processes generation weaken our achievement obtained aspect water pollution control just more and more significantly.

For a long time, be engaged in the engineering technical personnel of organic sewage Processing Technology Research and popularization, all how in sewage treatment process, reduce innoxious, the minimizing and the stabilization of the treatment and disposal of the output of excess sludge and excess sludge in research hardy always, and released a large amount of achievements in research in recent years, and these achievements all are faced with huge resistance on promoting, and reason is exactly to promote the running cost that these technology have all further increased the organic sewage treating processes to a great extent.

In traditional organic sewage treatment process, all be by adjusting the organism in the organic sewage, the ratio of ammonia nitrogen and phosphorus, keep nutrition and the energy balance of microorganism, promote the breeding of microorganism, strengthen the active of pollution substance in microorganism absorption and the degraded organic sewage, as seen, the purification of organic sewage, from realizing by producing a large amount of microorganisms in essence, and a large amount of microbies that purification organic sewage process produces, just remaining active sludge, and these remaining active sludge are mishandling, might cause the pollution of another kind of form again, therefore, new technology in that a lot of organic sewages of releasing are in recent years handled all is placed on the generation that how to reduce residual active sludge to focus, does not influence again in the research of wastewater treatment efficiency, although the achievement of releasing is a lot, but the effect of practical application is limited, and a large amount of generations of residual active sludge still become a burden of organic sewage treating processes.In view of the situation, we have to examine closely again the speciality of the vital movement of microorganism in participating in the organic sewage treating processes.

Summary of the invention

Technical problem to be solved by this invention is, a kind of method of utilizing the organic sewage synthesizing polyhydroxyalkanoateby is provided, feature according to the vital movement that participates in the microorganism that organic sewage handles proposes existing organic sewage treatment process is carried out an improved scheme.In utilizing microbiological treatment organic sewage process, by the control reaction conditions, improve the semi-invariant of PHA in the microorganism cells body, the treating processes of organic sewage, no longer be to be purpose merely just to dispose of sewage, but the process that organic sewage is handled regarded as utilize organic sewage to do raw material, adopt microorganism synthetic means to reach the purpose of synthesizing polyhydroxyalkanoateby.

For achieving the above object, the present invention adopts following technical scheme to implement: a kind of method of utilizing the organic sewage synthesizing polyhydroxyalkanoateby, to in the process that organic sewage is handled, precipitate and collect the microbe obtain and be input to the container that is used to extract PHA, regulate the pH value in the 4-12 scope, add sherwood oil or chloroform, tetracol phenixin, the water-fast organic solvent of solvent wet goods in 1: 1 ratio, under 10-80 ℃ of condition of insulation, it is static to layering fully to mix the back, extracts organic phase; The organic phase of extracting is placed airtight drying plant drying and reclaims solvent, and the desciccate that obtains is PHA.

In sum, effect of the present invention is as follows: the present invention is a kind of microbiological treatment scheme of follow-on organic sewage, it is again a kind of method of utilizing organic sewage to produce PHA, the enforcement of this scheme, make the environment protection treating work of organic sewage no longer regard the organic substance in the sewage as burden, but organic sewage is considered as a kind of raw material of producing PHA, be the scheme that a cover can be realized the organic sewage recycling treatment to a great extent.Remaining residue after the extraction organic phase, it is so-called microbial cell fragment, because the intravital PHA of cell is suggested, the protein content of these microorganism cells fragments can obviously improve, can be used as the raw material that extracts aminoacids complex, also can by after the fermentation slaking as the high-quality fertilizer material.

Utilize this method to handle organic sewage, not only obtain PHA, and need not carry out special cultivation and domestication to the microbial strains that participates in the organic sewage processing, bacterial classification according to the method gained of general microbiological treatment organic sewage culturing micro-organisms gets final product, production process need not be added other bacterial classifications, make the production cost of biological process synthesizing polyhydroxyalkanoateby cheaper, operate simpler.

Embodiment

Below in conjunction with embodiment the present invention is described in further detail: a kind of method of utilizing the organic sewage synthesizing polyhydroxyalkanoateby, precipitation is collected the microbe that obtains be input to the container that is used to extract PHA, regulate the pH value in the 4-12 scope, in 1: 1 or add sherwood oil or chloroform, tetracol phenixin, the water-fast organic solvent of solvent wet goods greater than 1: 1 ratio, under 10-80 ℃ of condition of insulation, it is static to layering fully to mix the back, extracts organic phase; The organic phase of extracting is placed airtight drying plant drying and reclaims solvent, and the desciccate that obtains is PHA.

Generally speaking, the microorganism that participates in the organic sewage processing belongs to unicellular microorganism mostly, these microbies contain amounts of protein, accounting for about 50% of cell paste dry weight, secondly is the ester class, accounts for about 20% of cell paste dry weight, ester class wherein is the intravital energy storage material of microorganism, many forms with PHA exist, and have data to prove, this material has good bio-compatibility, biodegradability, biocompatibility and optical property etc.Simultaneously, a large amount of experimental results show that, participate in the microorganism that organic sewage is handled, when the nutritional condition nonequilibrium state, the cumulative PHA can reach more than 70% in the microorganism cells body, so, if in utilizing microbiological treatment organic sewage process, can be by suitably controlling reaction conditions, improve the semi-invariant of PHA in the microorganism cells body, the treating processes of organic sewage is being not a purpose to dispose of sewage merely just, but have reason fully the process that organic sewage is handled regarded as and utilize organic sewage to do raw material, adopt the method for microorganism synthetic means synthesizing polyhydroxyalkanoateby.

By microbiological treatment organic sewage synthesizing polyhydroxyalkanoateby, key is the reaction conditions of controlling microbial processing organic sewage process, make the ratio of carbon in the sewage, nitrogen, phosphorus no longer satisfy 100: 5: 1 relation, make wherein nitrogen, phosphorus content more on the low side as far as possible, just can reach the purpose that improves PHA semi-invariant in the microorganism cells body.A large amount of experimental results show that, in the microbiological treatment process of organic sewage, the time that organic substance in the microorganism cells body absorption sewage needs is very of short duration, all the other mosts of the time all are the time that microorganism is converted into the organic substance that adsorbs the life Metabolic activity of cell paste, therefore, if what the microbiological treatment process of organic sewage adopted is sequence batch (production, in control nutritional imbalance condition, control the suitable reaction times again, detect the interior PHA semi-invariant of microorganism cells body and reach best, the requirement of sewage disposal index conformance with standard, promptly can be with the microbe quiescent setting, the supernatant discharging, get the microorganism cells body and function that partly precipitated gets off again and extract PHA, remaining microorganism cells body is used to handle the next batch organic sewage as bacterial classification; If what the microbiological treatment process of organic sewage adopted is continous way production, then can drain partially mixed liquid at the middle suitable position of whole microbial reaction system, behind the quiescent setting supernatant is returned Sewage treatment systems, precipitation is used for extracting PHA.

Claims

1. method of utilizing the organic sewage synthesizing polyhydroxyalkanoateby, it is characterized in that, to in the process that organic sewage is handled, precipitate and collect the microbe obtain and be input to the container that is used to extract PHA, regulate the pH value in the 4-12 scope, add sherwood oil or chloroform, tetracol phenixin, the water-fast organic solvent of solvent wet goods in 1: 1 ratio, under 10-80 ℃ of condition of insulation, static after fully mixing to layering, extract organic phase; The organic phase of extracting is placed airtight drying plant drying and reclaims solvent, and the desciccate that obtains is PHA.