CN101244093A - Method of preparing shiitake fungus polyoses oral liquid - Google Patents
Method of preparing shiitake fungus polyoses oral liquid Download PDFInfo
- Publication number
- CN101244093A CN101244093A CNA2008100338084A CN200810033808A CN101244093A CN 101244093 A CN101244093 A CN 101244093A CN A2008100338084 A CNA2008100338084 A CN A2008100338084A CN 200810033808 A CN200810033808 A CN 200810033808A CN 101244093 A CN101244093 A CN 101244093A
- Authority
- CN
- China
- Prior art keywords
- oral liquid
- concentrated
- shiitake fungus
- supernatant
- polyoses oral
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Landscapes
- Medicines Containing Plant Substances (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Abstract
The invention relates to a preparation method of mushroom polysaccharide oral liquid, which comprises the following steps: slicing mushroom, heating extraction, concentration, alcohol precipitation, high-speed centrifugation, concentration and getting small molecular weight mushroom polysaccharide oral liquid. The oral liquid is characterized in that the obtained mushroom polysaccharide oral liquid removes big molecular structure unsuitable for absorption, therefore, the preparation method has the advantages of preventing gastrointestinal inflation, promoting gastrointestinal absorption, enhancing effect of the product and generating no side effect, facilitating the use, and guaranteeing the quality.
Description
Technical field
The present invention relates to a kind of preparation method of shiitake fungus polyoses oral liquid, the treatment that can be used as cancer-resisting and improve immunity belongs to technical field of Chinese medicines.
Background technology
Lentinus Edodes is the traditional famous edible fungi of China, artificial domesticating cultivation the earliest in the world, Lentinus Edodes is nutritious, delicious flavour, be regarded as " king in the mushroom ", contain protein 18.64%, fat 4.8%, carbohydrate 71% in the dried thin mushroom, in ten several amino acids that Lentinus Edodes contains, the aminoacid that 7 kinds of needed by human such as isoleucine, lysine, phenylalanine, methionine, threonine, valine are arranged also contains vitamin D, B1, B2, PP and mineral salt and crude fibre etc.Lentinus Edodes has very high medical value, as far back as the medical value of oneself record Lentinus Edodes of China " daily vegetation " in 1329, points out that Lentinus Edodes has the merit of " QI invigorating is not hungry, controls the wind removing blood stasis ".Japan did more research to the therapeutical effect of Lentinus Edodes afterwards, proposed the growth that Lentinus Edodes can help child's skeleton and tooth, and control baby rickets promotes the health normal development.In the middle period 70 year last century, scientific and technological circle have set foot on the track that lentinan is carried out modern scientific research.
Modern study shows, contained lentinan and double stranded RNA in the Lentinus Edodes extract has stronger anti-cancer and cancer-preventing effect, is applicable to the food therapy of the multiple malignant tumor that comprises leukemia.
The shiitake fungus polyoses oral liquid that conventional preparation method obtains contains the macromolecular substances that more human body intestines and stomach is difficult for absorption, and effect is not remarkable after taking, and has stomach malaise symptom.
Summary of the invention
The purpose of this invention is to provide a kind of preparation method that is easy to the shiitake fungus polyoses oral liquid of human body the intestines and stomach absorption.For realizing above purpose, technical scheme of the present invention provides a kind of preparation method of shiitake fungus polyoses oral liquid, its
Be characterised in that, Lentinus Edodes through section, heating extraction, concentrate, precipitate with ethanol, high speed centrifugation, concentrate and obtain the small-molecular weight shiitake fungus polyoses oral liquid, the steps include:
The first step. get clean mushroom fruiting body, be ground into the fritter grain,, throw to extracting still through tap water flushing dust, add the deionized water that Lentinus Edodes weight 15-20 doubly measures, heat up and be heated to 100 ℃ of extractions, extraction stops after 3-5 hour, be cooled to room temperature, discharging, liquid-solid separation;
Second step. clear liquid is delivered to concentrated post and is concentrated into Lentinus Edodes weight 3-4 doubly, and filtering residue adds the deionized water that 10-15 doubly measures again, repeats the said extracted condition and extracts 4 times, and filtering residue is abandoned it;
The 3rd step. supernatant merges the 3-4 times of concentrated solution that is concentrated into mushroom fruiting body weight, under room temperature, stirring condition, add concentrated solution volume 1: the concentration of 0.4-1 is 95% medicinal alcohol, continue to stir 15-30 minute, static 6-24 hour, high speed centrifugation separates supernatant, and rotating speed is 10000-20000r/min, and filtering residue is abandoned it;
The 4th step. supernatant send concentrated, be concentrated in the scope of 2/5-3/7 of supernatant volume, this shiitake fungus polyoses oral liquid.
Preparation method utilization deionized water of the present invention makes extraction effectively fully, and by precipitate with ethanol, will be not easy to macromolecular substances and the removal of some soluble impurities that intestines and stomach absorbs.
Advantage of the present invention is the absorption that resulting shiitake fungus polyoses oral liquid is easy to the human body intestines and stomach, has strengthened the curative effect of this product, and have no side effect, method is easy, quality is guaranteed.
The specific embodiment
The invention will be further described below in conjunction with embodiment.
Embodiment
Get clean mushroom fruiting body, be ground into the fritter grain, through tap water flushing dust, throw to extracting still, add the deionized water of 20 times of amounts of Lentinus Edodes weight, intensification is heated to 100 ℃ of extractions, extraction stops after 5 hours, is cooled to room temperature, discharging, liquid-solid separation, clear liquid is delivered to concentrated post and is concentrated into 4 times of Lentinus Edodes weight, and filtering residue adds the deionized water of 12 times of amounts again, repeats the said extracted condition and extracts 4 times, filtering residue is abandoned it, supernatant merges 4 times of concentrated solutions that are concentrated into mushroom fruiting body weight, at room temperature, under the stirring condition, 1: 1 amount concentration of adding concentrated solution volume is 95% medicinal alcohol, continue to stir 30 minutes, after static 8 hours, high speed centrifugation separates supernatant, and rotating speed is 16000r/min, filtering residue is abandoned it, supernatant send concentrated, be concentrated in 3/7 the scope of supernatant volume, this lentinan syrup, press the general preparation method of oral liquid, canned, seal, sterilization promptly gets oral liquid.
The test of pesticide effectiveness that the shiitake fungus polyoses oral liquid that utilizes this preparation method to make is done
The tumor suppression test: get 4-5 Kunming mouse in age in week, body weight 20 ± 5g, the male and female dual-purpose, random packet, 12 every group, with S
180It is 5 * 10 that tumor liquid is diluted to cell concentration with normal saline
6The cell suspension of/0.2ml, ip injects.Irritate stomach respectively and give the shiitake fungus polyoses oral liquid (1g/kg) that makes with general technology shiitake fungus polyoses oral liquid (1g/kg) and this preparation method.Once a day; Three weeks of logotype.Observe of the influence of three kinds of medicines to the tumor-bearing mice life span.The result shows that the oral liquid life span that this preparation method makes obviously is longer than the general technology shiitake fungus polyoses oral liquid.
Oral liquid of the present invention is to the influence of tumor-bearing mice life span
| The animal grouping | Mean survival time (n=14) |
| The general technology shiitake fungus polyoses oral liquid | 11.5 my god |
| This preparation method shiitake fungus polyoses oral liquid | 17 days |
This technology makes shiitake fungus polyoses oral liquid relatively with general technology
| Title | This preparation method shiitake fungus polyoses oral liquid | General technology makes shiitake fungus polyoses oral liquid |
| Molecular weight ranges | 2-100 ten thousand dalton | ＞100 ten thousand dalton are main |
| Active substance content | With | Low |
| Side effect | Do not have | Have |
| Impurity | Few | Many |
Claims (1)
1. the preparation method of a shiitake fungus polyoses oral liquid is characterized in that, Lentinus Edodes through section, heating extraction, concentrate, precipitate with ethanol, high speed centrifugation, concentrate and obtain the small-molecular weight shiitake fungus polyoses oral liquid, the steps include:
The first step. get clean mushroom fruiting body, be ground into the fritter grain,, throw to extracting still through tap water flushing dust, add the deionized water that Lentinus Edodes weight 15-20 doubly measures, heat up and be heated to 100 ℃ of extractions, extraction stops after 3-5 hour, be cooled to room temperature, discharging, liquid-solid separation;
Second step. clear liquid is delivered to concentrated post and is concentrated into Lentinus Edodes weight 3-4 doubly, and filtering residue adds the deionized water that 10-15 doubly measures again, repeats the said extracted condition and extracts 4 times, and filtering residue is abandoned it;
The 3rd step. supernatant merges the 3-4 times of concentrated solution that is concentrated into mushroom fruiting body weight, under room temperature, stirring condition, add concentrated solution volume 1: the concentration of 0.4-1 is 95% medicinal alcohol, continue to stir 15-30 minute, static 6-24 hour, high speed centrifugation separates supernatant, and rotating speed is 10000-20000r/min, and filtering residue is abandoned it;
The 4th step. supernatant send concentrated, be concentrated in the scope of 2/5-3/7 of supernatant volume, this shiitake fungus polyoses oral liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100338084A CN101244093A (en) | 2008-02-22 | 2008-02-22 | Method of preparing shiitake fungus polyoses oral liquid |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100338084A CN101244093A (en) | 2008-02-22 | 2008-02-22 | Method of preparing shiitake fungus polyoses oral liquid |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101244093A true CN101244093A (en) | 2008-08-20 |
Family
ID=39944912
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2008100338084A Pending CN101244093A (en) | 2008-02-22 | 2008-02-22 | Method of preparing shiitake fungus polyoses oral liquid |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101244093A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103948621A (en) * | 2014-04-24 | 2014-07-30 | 上海慈瑞医药科技有限公司 | Lentinan oral preparation and preparation method thereof |
-
2008
- 2008-02-22 CN CNA2008100338084A patent/CN101244093A/en active Pending
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103948621A (en) * | 2014-04-24 | 2014-07-30 | 上海慈瑞医药科技有限公司 | Lentinan oral preparation and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102640829B (en) | Tea paste with myocardial ischemia function improving function and preparation method of tea paste | |
| CN102960789A (en) | Isatis root and red date concentrated slurry product | |
| CN107184966A (en) | A kind of pharmaceutical composition for preventing and treating diabetes and its complication | |
| CN109601795A (en) | Using penthorum chinense pursh as composition alcohol-decomposing beverage of primary raw material and preparation method thereof | |
| CN108813501B (en) | Health-preserving honey paste with functions of clearing heat, moistening lung, relieving cough, reducing phlegm, relieving asthma and regulating human body functions | |
| KR20170116532A (en) | Maca and date palms, Ganoderma lucidum, perform exercises containing fermented antler extract as an active ingredient skills enhancement and promotion fatigue complex composition | |
| CN110075266A (en) | One breeding kidney reinforces the kidney the formula to fill blood and preparation method | |
| CN103725555A (en) | Wild banana and oxhorn banana health-care wine for assisting blood pressure reduction and preparation method thereof | |
| CN102604802B (en) | Preparation method of originally ecological bamboo-germanium nutritional wine | |
| KR102659842B1 (en) | Health functional food composition for improvement of bowel function and relief of contipation comprising psyllium husk and complex extract, and manufacturing method thereof | |
| CN100467592C (en) | Preparation of highland barley SOD an conditioning food containing highland barley SOD | |
| CN105192820A (en) | Blood-fat-reducing cordyceps militaris polysaccharide beverage and preparing method thereof | |
| CN102119939A (en) | Angiosperm bee pollen water extract for treating fatty liver and preparation method and application thereof | |
| KR102235563B1 (en) | Composition for fatigue recovery and sexual function improvement containing high content of arginine | |
| CN102613648B (en) | Preparation method of original ecological bamboo germanium nutrient solution | |
| KR101088711B1 (en) | A composition for prevention or alleviation of obesity containing Astragalus membranaceus extract manufactured by using enzymatic hydrolysis | |
| KR102433586B1 (en) | Food composition for relieving hangover and improving hepatic function | |
| CN101244093A (en) | Method of preparing shiitake fungus polyoses oral liquid | |
| CN108157545A (en) | A kind of guava leaf liquid beverage and preparation method thereof | |
| CN103652923A (en) | Preparation method of cordyceps sinensis lozenge | |
| CN113519737A (en) | Polypeptide beverage for dispelling effects of alcohol and protecting liver and preparation method thereof | |
| CN101036509A (en) | Physalis alkekengi can | |
| CN106668332B (en) | Passion fruit buccal tablet and preparation method thereof | |
| KR20090098254A (en) | Method for producing artemisia extract by fermentation | |
| CN109876053A (en) | A kind of acne-removing inflammation-diminishing, removing toxic substances of clearing liver prescription and its preparation method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20080820 |