CN101243769A - Cultivation method for flax hybridization by using male sterile of flax - Google Patents
Cultivation method for flax hybridization by using male sterile of flax Download PDFInfo
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- CN101243769A CN101243769A CNA2007100802248A CN200710080224A CN101243769A CN 101243769 A CN101243769 A CN 101243769A CN A2007100802248 A CNA2007100802248 A CN A2007100802248A CN 200710080224 A CN200710080224 A CN 200710080224A CN 101243769 A CN101243769 A CN 101243769A
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Abstract
The invention relates to a cultivating method to utilize flax male sterility to hybrid flax, belonging to the field of crop cultivation. Heterosis utilization is an effective way to improve the yield of flax, but the main obstacle in prior suitable heterosis utilization is the lack of excellent flax male sterile line. The invention is characterized in that, excellent temperature sensitive sterile line successfully selected and bred through antibiotic mutation is utilized for hybrid breeding and hybrid production; the cross combination with obvious yield heterosis is bred; the method to produce sterile line at high temperature area and produce hybrid F1 seed together with a restore line at low temperature area is invented. The cultivating method has the advantages of simple propagation, low production cost, wide sterile line restoring sources and obvious heterosis.
Description
Technical field
The present invention relates to the crop genetics field, especially the hybridization breeding method of flax.
Background technology
F1 will be to improve the effective way of flax output for 25-40% even higher hybrid vigour, heterosis utilization are arranged between flax kind.Flax is a self pollination crop, male sterile line is the key of heterosis utilization, nineteen twenty-one Bateson and Cairdner identify flax cytoplasmic male sterile line first, but the petal of this male sterile line can not fully launch, and cross pollination is subjected to obstacle, is difficult to use in the production of hybrid seeds, no direct value on producing, after this, the report (Kumar and Singn, 1970 that also have the fully extended flax cytoplasmic male sterile line of petal; Thomsom, 1977), but on crossbreed utilizes, do not see the report of achieving success.1973, dominant genic male sterile flax was found on China Chen Hong mountain etc., but owing to be difficult to recover and keep, failed to be applied to crossbreed and produce.At present, the shortage of flax male sterile resource of suitable heterosis utilization is the major obstacle of flax heterosis utilization research.
Summary of the invention
The objective of the invention is provides a kind of breeding method that utilizes flax male sterile line to produce cross breeding flax in order to solve flax F1 hybridization mass production problem.
The technical scheme that realizes the foregoing invention purpose is as follows:
Utilize flax male sterile line to produce cross breeding flax F1 kind submethod:
(1) sterile line breeding, by after the antibiotic mutagenesis to male sterile mutant systematic breeding, the conversion of two fertility of seed selection 1s and 5s better, the comprehensive good male sterile line of proterties,
(2) breeding of hybrid combination is hybridized male sterile line and conventional variety in a large number, filters out the hybrid combination of two yield increased group volume increase,
(3) the production utilization of crossbreed is produced male sterile line in the high temperature area, produces hybridization F1 seed together at low temp area and recovery system again.
The main points of technical solution of the present invention are to utilize a kind of low temperature of novel male sterile line to keep fertility, the temperature-sensing property that the high temperature fertility is better recovered, and fertility is controlled by recessive single-gene, heredity is simple, recover easily, recover system extensively, male parent is selected the wide characteristics in field, produce male sterile line in the high temperature area, produce hybridization F1 seed together at low temp area and recovery system again.
Beneficial effect of the present invention
This invention has the following advantages: 1. realized the large-scale production crossbreed.2. sterile line propagation is simple, and production cost is low.3. it is extensive that male sterile line recovers the source, and general kind (being) all can be used as it and recovers system, the combo freedom, and heterosis, hybrid vigor is obvious.
Embodiment
Now technical scheme of the present invention is described further by following embodiment.
After antibiotic mutagenesis, to male sterile mutant systematic breeding.Gansu Province inferior ninth-seeded M1 kind of antibiotic treatment is planted the field, seek male sterile, to the sterile plant individual plant results of finding at the florescence; M2 presses the plant plantation, and the sterile plant of separating is identified, selects good male sterile individual plant, carries out artificial bagging when fertility restorer, self-fertility, individual plant results; M3 presses the plant plantation; M4---M6 identifies at different ecosite (seeing Table 1 and 2) respectively and selects, carry out fertility identifies in the high-temperature region, identify and selection in the capable sterility of low-temperature range, select, it is better to select 1S and two fertility conversions of 5s of breeding, the male sterile line that comprehensive proterties is good (the experimental data row see Table 3).
The basic geographical ecological condition of table 15 test sites
Table 2 fertility and temperature comparison sheet
The economical character of table 3 male sterile line and outcrossing
| Title material | Plant height cm | Technology length cm | Effective branch (individual) | Individual plant fruit number | Thousand kernel weight | Flowering stage | Maturing stage | Outcrossing seed-setting rate |
| Male sterile line | 55～67 | 32～36 | 4～5 | 24 | 7.0g | 5/6 | 20/7 | About 6 |
| Inferior No. 9 of Gansu Province | 53～62 | 31～38 | 4～5 | 25 | 7.1.g | 5/6 | 20/7 |
Male sterile line and conventional variety are hybridized in a large number (seeing Table 4), filtered out assorted 03 hybrid combination that remarkable contrast is increased production with assorted 04 two output.For assorted 03 parental combination is 1S * 9873, recover in this combination be 9873 be a precocity, of short stem, anti-ly fall, the big grain of big fruit, high resistance to wilt good kind, yielding ability is outstanding, with the ripe property of male sterile line basically identical, in crossbreed production, does not need intermission seeding.Male sterile line 1S is selected by the male sterile sudden change system of antibiotic mutagenesis to form, and its fertility is to responsive to temperature, and yielding ability is good, long branch, and individual plant fruit number is many, and precocity is of short stem resistant to lodging, high resistance to wilt good.Assorted 04 parental combination is 1S * 91-197-8, and recovering in this combination is that 91-197-8 is a precocity, high resistance to wilt good kind, and yielding ability is outstanding, and yield stability is good, with the ripe property of male sterile line basically identical, in crossbreed produces, does not need intermission seeding.Male sterile line 1S is selected by the male sterile sudden change system of antibiotic mutagenesis to form, and its fertility is to responsive to temperature, and yielding ability is good, long branch, and individual plant fruit number is many, and precocity is of short stem resistant to lodging, high resistance to wilt good.The production utilization of crossbreed: utilize the low temperature of novel male sterile line to keep fertility, the temperature-sensing property that the high temperature fertility is recovered preferably, and fertility is controlled by recessive single-gene, heredity is simple, recover easily, recover system extensively, male parent is selected the wide characteristics in field, (daily mean temperature is greater than 20 ℃) produce male sterile line in the high temperature area, produce hybridization F1 seed together at low temp area (daily mean temperature is lower than 17 ℃) and recovery system again.
Table 4 hybrid combination output and hybrid vigour performance
Claims (1)
1. breeding method that utilizes flax male sterile cross breeding flax, it is characterized by: (1) sterile line breeding, by after the antibiotic mutagenesis to male sterile mutant systematic breeding, two fertility conversions of seed selection 1s and 5s better, the comprehensive good male sterile line of proterties, (2) breeding of hybrid combination, male sterile line and conventional variety are hybridized in a large number, filter out the hybrid combination of two yield increased group volume increase, (3) the production utilization of crossbreed, produce male sterile line in the high temperature area, produce hybridization F1 seed together at low temp area and recovery system again.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100802248A CN101243769A (en) | 2007-02-14 | 2007-02-14 | Cultivation method for flax hybridization by using male sterile of flax |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007100802248A CN101243769A (en) | 2007-02-14 | 2007-02-14 | Cultivation method for flax hybridization by using male sterile of flax |
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| CN101243769A true CN101243769A (en) | 2008-08-20 |
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| CNA2007100802248A Pending CN101243769A (en) | 2007-02-14 | 2007-02-14 | Cultivation method for flax hybridization by using male sterile of flax |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119662A (en) * | 2010-12-24 | 2011-07-13 | 黑龙江八一农垦大学 | Polyploidy induction method for fiber linum usitatissimum under isolated culture condition |
| CN104472340A (en) * | 2014-11-11 | 2015-04-01 | 甘肃省农业科学院作物研究所 | Breeding method of flax male sterility line with high stigma exertion rate and outcrossing rate |
| CN104663228A (en) * | 2015-03-16 | 2015-06-03 | 甘肃省农业科学院作物研究所 | Production method for core seeds and stock seeds of thermo-sensitive male sterile line 1S of flax |
| CN106332772A (en) * | 2016-08-24 | 2017-01-18 | 张掖市农业科学研究院 | Hybrid seed production method of flax |
| WO2017080119A1 (en) * | 2015-11-13 | 2017-05-18 | 上海师范大学 | Method of culturing photo/thermo-sensitive genic sterile rice |
| CN108575732A (en) * | 2018-05-22 | 2018-09-28 | 黑龙江省农业科学院经济作物研究所 | A kind of method of quick initiative apomixia flex seed |
-
2007
- 2007-02-14 CN CNA2007100802248A patent/CN101243769A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102119662A (en) * | 2010-12-24 | 2011-07-13 | 黑龙江八一农垦大学 | Polyploidy induction method for fiber linum usitatissimum under isolated culture condition |
| CN102119662B (en) * | 2010-12-24 | 2012-06-06 | 黑龙江八一农垦大学 | Polyploidy induction method for fiber linum usitatissimum under isolated culture condition |
| CN104472340A (en) * | 2014-11-11 | 2015-04-01 | 甘肃省农业科学院作物研究所 | Breeding method of flax male sterility line with high stigma exertion rate and outcrossing rate |
| CN104663228A (en) * | 2015-03-16 | 2015-06-03 | 甘肃省农业科学院作物研究所 | Production method for core seeds and stock seeds of thermo-sensitive male sterile line 1S of flax |
| CN104663228B (en) * | 2015-03-16 | 2016-08-03 | 甘肃省农业科学院作物研究所 | One grows flax Thermo-sensitive male sterile line 1S core seeds and the production method of original seed |
| WO2017080119A1 (en) * | 2015-11-13 | 2017-05-18 | 上海师范大学 | Method of culturing photo/thermo-sensitive genic sterile rice |
| CN106332772A (en) * | 2016-08-24 | 2017-01-18 | 张掖市农业科学研究院 | Hybrid seed production method of flax |
| CN108575732A (en) * | 2018-05-22 | 2018-09-28 | 黑龙江省农业科学院经济作物研究所 | A kind of method of quick initiative apomixia flex seed |
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Open date: 20080820 |