CN101239199A - Self-adhering type chitosan haemostatic membrane and preparation thereof - Google Patents
Self-adhering type chitosan haemostatic membrane and preparation thereof Download PDFInfo
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- CN101239199A CN101239199A CNA2008100611781A CN200810061178A CN101239199A CN 101239199 A CN101239199 A CN 101239199A CN A2008100611781 A CNA2008100611781 A CN A2008100611781A CN 200810061178 A CN200810061178 A CN 200810061178A CN 101239199 A CN101239199 A CN 101239199A
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- chitosan
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- citric acid
- haemostatic membrane
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2400/00—Materials characterised by their function or physical properties
- A61L2400/04—Materials for stopping bleeding
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Abstract
The present invention discloses a self-adhesive type chitosan hemostatic film, which is composed of components by weight percentage: 85-93% chitosan, 5-10% glycerin, 2-5% citric acid. The preparing steps includes: weighing chitosan, glycerin and citric acid, then adding chitosan into acetum with 1.5%-2% mass concentration, mixing to chitosan dissolved, adding glycerin and citric acid again, mixing to mixed solution; pouring said solution into glass mould and self-levelling, drying to form film, and cooling to room temperature; supporting the mould with film in closed container filling with absolute ethyl alcohol, and suffocating 2-3 hours, exposing film from mould. The film has no bacterium, and the self-adhesive surface absorbs moisture and forms gel instantly when contacts with blood or body fluid after is planted into body, the gel has adhesive and has effects of hemostasia and healing. Other side of the film has no adhesive and acts on isolation function with periphery.
Description
Technical field
The present invention relates to haemostatic membrane and preparation method thereof, especially self-adhering type chitosan haemostatic membrane and preparation method thereof.
Background technology
Chitosan has good film property and excellent biological compatibility, so be widely used in manufacture of intraocular skin, barrier film capable of preventing postoperative adhesions etc.Have recently and studies show that more chitosan also has good hemostasia effect, its hemostatic mechanism is to be different from conventional waterfall clotting mechanism fully, chitosan mainly is by erythrocytic absorption and coagulation are realized its anastalsis, and it also has certain influence to platelet, Fibrinogen in addition.And chitosan is again a kind of biodegradable natural macromolecular material, the degraded that can be absorbed by the body that implants, and its catabolite has no side effect to human body, so the absorbable hemostatic material that chitosan is a kind of ideal to implant.But because chitosan has distinctive molecular structure, intermolecular active force is very big, causes chitosan not adopt chitosan-acetic acid solution The tape casting drying at present mostly, to cross in the alkali and technology system film with traditional macromolecular material film technique film forming.This film has two defectives when using as haemostatic membrane, and the first, when alkali liquor is handled strong swelling to take place flexible crossing for this film, causes film uneven, and film properties becomes hard and crisp, can't use.The second, this film uses inconvenience, because of the film no adhesion, can not paste with organizing voluntarily, can't fix in vivo.Therefore chitosan material really to be become haemostatic membrane material in the body with using value clinically,, must have stronger operability simultaneously, be convenient to the doctor and in art, operate except satisfying the clinical requirement at aspects such as haemostatic effect and bio-toxicities.
Summary of the invention
The objective of the invention is to provide a kind of good shell of stickup certainly polysaccharide haemostatic membrane of the haemostatic effect that implants and preparation method thereof that has.
Of the present invention from pasting shell polysaccharide haemostatic membrane, its component and weight percent content thereof are:
Chitosan 85～93%
Glycerol 5～10%
Citric acid 2～5%
Preparation method from pasting shell polysaccharide haemostatic membrane may further comprise the steps:
1) presses the amount of ingredient, get chitosan, glycerol and citric acid, earlier chitosan is joined in mass concentration 1.5%～2% acetum, be stirred to the chitosan dissolving, add glycerol and citric acid again, stir, make mixed solution;
2) above-mentioned mixed liquor is poured in the glass mold from levelling, put into baking oven then, take out mould, be cooled to room temperature at 40～75 ℃ of following drying and forming-films;
3) with step 2) the die frame with film in filling the hermetic container of dehydrated alcohol, contact liq, stifling 2～3 hours, take out mould, take film off, be chitosan haemostatic membrane from adhesive type.
The thickness of haemostatic membrane is by the consumption decision of die area size and mixed solution.
The physicochemical property of the chitosan haemostatic membrane of self-adhering-type of the present invention:
The chitosan haemostatic membrane of self-adhering-type of preparation is apparent evenly, water white transparency, thickness 60 ± 30 μ m, and moisture content≤20% has certain tensile strength and elongation at break; The pH value 6.5～7 of film, film ash (mass percent)≤2%, insoluble matter in the film (mass percent)≤1.5%, heavy metal total content (in Pb)≤10ppm in the film;
The biological assessment of the chitosan haemostatic membrane of self-adhering-type:
Film is aseptic, when implanting, and infective inflammation reaction nothing but, no sensitization of skin reaction, no cytotoxicity reaction, no thermal source and stimulation sensitivity response, hereditary-less toxicity.
The autohension and the hemostasia effect of the chitosan haemostatic membrane of self-adhering-type:
The self-adhesion face of film can absorb moisture immediately when contacting with blood or body fluid and form gel, has viscosity, sticks on the wound voluntarily, and the gel of its chitosan plays erythrocyte absorption and agglutinative effect, has the effect of hemostasis, promotion healing.And the another side of film is fumigated processing through ethanol, and no adhesion has played the shape of keeping film, temporarily keeps film strength, and plays isolated effect with perienchyma.
The specific embodiment
Further specify the present invention below in conjunction with embodiment.
Embodiment 1
Certainly the stickup shell polysaccharide haemostatic membrane of invention contains (by weight)
Chitosan 90%
Glycerol 10%
Citric acid 2%
Preparation method, step is as follows:
1) amount by ingredient takes by weighing chitosan, glycerol and citric acid, earlier chitosan is joined in mass concentration 2% acetum, is stirred to the chitosan dissolving, adds glycerol and citric acid again, stirs, and makes mixed solution;
2) above-mentioned mixed liquor is poured in the glass mold from levelling, put into baking oven then, take out mould, be cooled to room temperature 40 ℃ of down dry 6 hours film forming;
3) with step 2) the die frame with film in filling the hermetic container of dehydrated alcohol, contact liq, stifling 3 hours, take out mould, take film off, be chitosan haemostatic membrane from adhesive type.
The moisture content of haemostatic membrane is 15%, the pH value 6.7 of film, and film ash≤2%, insoluble matter 0.05% in the film, heavy metal total content≤10ppm in the film.
The chitosan haemostatic membrane that makes shows through the animal body experiment, stick on the wound immediately voluntarily when the film one side contacts with body fluid with blood, and good buffer action has played in the another side of film and perienchyma, compares with matched group to have tangible haemostatic effect.
Embodiment 2
Certainly the stickup shell polysaccharide haemostatic membrane of invention contains (by weight)
Chitosan 85%
Glycerol 8%
Citric acid 5%
1) amount by ingredient takes by weighing chitosan, glycerol and citric acid, earlier chitosan is joined in mass concentration 1.5% acetum, is stirred to the chitosan dissolving, adds glycerol and citric acid again, stirs, and makes mixed solution;
2) above-mentioned mixed liquor is poured in the glass mold from levelling, put into baking oven then, take out mould, be cooled to room temperature 75 ℃ of down dry 4 hours film forming;
3) with step 2) the die frame with film in filling the hermetic container of dehydrated alcohol, contact liq, stifling 2 hours, take out mould, take film off, be chitosan haemostatic membrane from adhesive type.
The moisture content of haemostatic membrane is 15%, the pH value 6.5 of film, and film ash≤2%, insoluble matter 0.05% in the film, heavy metal total content≤10ppm in the film.
The chitosan haemostatic membrane that makes shows through the animal body experiment, stick on the wound immediately voluntarily when the film one side contacts with body fluid with blood, and good buffer action has played in the another side of film and perienchyma, compares with matched group to have tangible haemostatic effect.
Embodiment 3
Certainly the stickup shell polysaccharide haemostatic membrane of invention contains (by weight)
Chitosan 93%
Glycerol 5%
Citric acid 3%
1) amount by ingredient takes by weighing chitosan, glycerol and citric acid, earlier chitosan is joined in mass concentration 1.5% acetum, is stirred to the chitosan dissolving, adds glycerol and citric acid again, stirs, and makes mixed solution;
2) above-mentioned mixed liquor is poured in the glass mold from levelling, put into baking oven then, take out mould, be cooled to room temperature 55 ℃ of down dry 5 hours film forming;
3) with step 2) the die frame with film in filling the hermetic container of dehydrated alcohol, contact liq, stifling 2.5 hours, take out mould, take film off, be chitosan haemostatic membrane from adhesive type.
The moisture content of haemostatic membrane is 15%, the pH value 6.6 of film, and film ash≤2%, insoluble matter 0.05% in the film, heavy metal total content≤10ppm in the film.
The chitosan haemostatic membrane that makes shows through the animal body experiment, stick on the wound immediately voluntarily when the film one side contacts with body fluid with blood, and good buffer action has played in the another side of film and perienchyma, compares with matched group to have tangible haemostatic effect.
Claims (2)
1. self-adhering type chitosan haemostatic membrane is characterized in that its component and weight percent content thereof are:
Chitosan 85～93%
Glycerol 5～10%
Citric acid 2～5%
2. the preparation method of self-adhering type chitosan haemostatic membrane according to claim 1 is characterized in that may further comprise the steps:
1) presses the amount of ingredient, get chitosan, glycerol and citric acid, earlier chitosan is joined in mass concentration 1.5%～2% acetum, be stirred to the chitosan dissolving, add glycerol and citric acid again, stir, make mixed solution;
2) above-mentioned mixed liquor is poured in the glass mold from levelling, put into baking oven then, take out mould, be cooled to room temperature at 40～75 ℃ of following drying and forming-films;
3) with step 2) the die frame with film in filling the hermetic container of dehydrated alcohol, contact liq, stifling 2～3 hours, take out mould, take film off, be chitosan haemostatic membrane from adhesive type.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100611781A CN101239199A (en) | 2008-03-18 | 2008-03-18 | Self-adhering type chitosan haemostatic membrane and preparation thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2008100611781A CN101239199A (en) | 2008-03-18 | 2008-03-18 | Self-adhering type chitosan haemostatic membrane and preparation thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN101239199A true CN101239199A (en) | 2008-08-13 |
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| Application Number | Title | Priority Date | Filing Date |
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| CNA2008100611781A Pending CN101239199A (en) | 2008-03-18 | 2008-03-18 | Self-adhering type chitosan haemostatic membrane and preparation thereof |
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Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110151061A1 (en) * | 2009-12-23 | 2011-06-23 | The Ohio State University | Food decoration |
| WO2016120620A1 (en) * | 2015-01-27 | 2016-08-04 | Medtrade Products Limited | Composition for a wound dressing |
| WO2016120621A1 (en) * | 2015-01-27 | 2016-08-04 | Medtrade Products Limited | Composition for a wound dressing |
| GB2537010A (en) * | 2015-01-27 | 2016-10-05 | Medtrade Products Ltd | Composition for a wound dressing |
| CN110152053A (en) * | 2018-01-12 | 2019-08-23 | 浙江舟山群岛新区旅游与健康职业学院 | Anti-inflammatory hemostasis compound and preparation method thereof |
| CN112316202A (en) * | 2020-11-13 | 2021-02-05 | 南通大学 | Shape memory hemostatic material and preparation method and application thereof |
| CN113265178A (en) * | 2021-05-19 | 2021-08-17 | 邓忠贤 | Chitin polysaccharide-containing ecological degradation membrane liquid and preparation method thereof |
-
2008
- 2008-03-18 CN CNA2008100611781A patent/CN101239199A/en active Pending
Cited By (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110151061A1 (en) * | 2009-12-23 | 2011-06-23 | The Ohio State University | Food decoration |
| GB2537008B (en) * | 2015-01-27 | 2019-10-23 | Medtrade Products Ltd | Composition for a wound dressing |
| AU2016210992B2 (en) * | 2015-01-27 | 2020-03-26 | Medtrade Products Limited | Composition for a wound dressing |
| GB2537010B (en) * | 2015-01-27 | 2019-10-16 | Medtrade Products Ltd | Composition for a wound dressing |
| GB2537008A (en) * | 2015-01-27 | 2016-10-05 | Medtrade Products Ltd | Composition for a wound dressing |
| GB2537010A (en) * | 2015-01-27 | 2016-10-05 | Medtrade Products Ltd | Composition for a wound dressing |
| CN107530470A (en) * | 2015-01-27 | 2018-01-02 | 医疗行业产品有限公司 | composition for wound dressing |
| CN107580506A (en) * | 2015-01-27 | 2018-01-12 | 医疗行业产品有限公司 | composition for wound dressing |
| WO2016120620A1 (en) * | 2015-01-27 | 2016-08-04 | Medtrade Products Limited | Composition for a wound dressing |
| GB2537009A (en) * | 2015-01-27 | 2016-10-05 | Medtrade Products Ltd | Composition for a wound dressing |
| US10973691B2 (en) | 2015-01-27 | 2021-04-13 | Medtrade Products Limited | Composition for a wound dressing |
| US10561533B2 (en) | 2015-01-27 | 2020-02-18 | Medtrade Products Limited | Composition for a wound dressing |
| GB2537009B (en) * | 2015-01-27 | 2019-10-23 | Medtrade Products Ltd | Composition for a wound dressing |
| AU2016210991B2 (en) * | 2015-01-27 | 2020-03-12 | Medtrade Products Limited | Composition for a wound dressing |
| WO2016120621A1 (en) * | 2015-01-27 | 2016-08-04 | Medtrade Products Limited | Composition for a wound dressing |
| US10639202B2 (en) | 2015-01-27 | 2020-05-05 | Medtrade Products Limited | Composition for a wound dressing |
| CN110152053A (en) * | 2018-01-12 | 2019-08-23 | 浙江舟山群岛新区旅游与健康职业学院 | Anti-inflammatory hemostasis compound and preparation method thereof |
| CN112316202A (en) * | 2020-11-13 | 2021-02-05 | 南通大学 | Shape memory hemostatic material and preparation method and application thereof |
| CN112316202B (en) * | 2020-11-13 | 2022-04-01 | 南通大学 | Shape memory hemostatic material and preparation method and application thereof |
| CN113265178A (en) * | 2021-05-19 | 2021-08-17 | 邓忠贤 | Chitin polysaccharide-containing ecological degradation membrane liquid and preparation method thereof |
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Open date: 20080813 |