CN101229315A - Method for producing salty rhizoma anemarrhenae - Google Patents
Method for producing salty rhizoma anemarrhenae Download PDFInfo
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- CN101229315A CN101229315A CNA2007101920010A CN200710192001A CN101229315A CN 101229315 A CN101229315 A CN 101229315A CN A2007101920010 A CNA2007101920010 A CN A2007101920010A CN 200710192001 A CN200710192001 A CN 200710192001A CN 101229315 A CN101229315 A CN 101229315A
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Abstract
The invention relates to a processing method of saline common anemarrhena rhizome, which efficiently solves the preparation of rhizome anemarrhenae into saline anemarrhenae decoction pieces, guarantees the high quality and stabilization of the quality, and has the specific technical proposal: taking rhizome anemarrhenae, decompressing and moist processing for a period of time, cutting into sections, drying in low temperature, adding salt water, stuffy running to the fully absorption of saline water, firing in the stir-fry vessel, putting to be cool, sealing and obtaining. The invention is simple in method, saving in time, easy in operation, and even in color of the prepared saline anemarrhenae tablets. Besides, compared with the traditional anemarrhenae processing method, the invention has the advantages of controlling the moisture and ash content within the scope regulated in the pharmacopeia, lowering the loss of the effective components sarsapogenin and mangiferin, preserving the efficacy, satisfying efficiently the need of the clinic to saline anemarrhenae decoction pieces, improving clinical efficacy, and providing specific technique parameter for the processing of the saline anemarrhenae.
Description
Technical field
The invention belongs to technical field of Chinese medicines, specifically relate to the concocting method of the Chinese medicine Rhizoma Anemarrhenae.
Technical background
The Rhizoma Anemarrhenae is the liliaceous plant Rhizoma Anemarrhenae (Anemarrhena asphodeloides) dry rhizome, is a kind of conventional Chinese medicine.Clinical common decoction pieces has the living Rhizoma Anemarrhenae, stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution.It is sliding sharp to give birth to Rhizoma Anemarrhenae bitter cold, is longer than clearing away heat-fire, promotes the production of body fluid and moisturizes, and the water of eliminating the pathogens from the lung, stomach should be given birth to usefulness especially.Be used for epidemic febrile disease high fever excessive thirst, cough due to lung-heat or cough due to yin deficiency are quenched one's thirst, constipation due to dry stool more.It is descending that but salt is processed priming, specially in going into kidney, can strengthen the effect of nourishing YIN to lower pathogenic fire, kind clearind deficient heat.Be usually used in hepatic and renal YIN deficiency, the osteopyrexia and fever due to the hyperactivity of deficient fire, night sweat seminal emission, spinal column are completed pain.The concocting method of the version Pharmacopoeia of the People's Republic of China regulation Rhizoma Anemarrhenae in 2005 has: the Rhizoma Anemarrhenae: remove impurity, clean, run through, cut sheet, drying, unhairing bits.Stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution: get Rhizoma Anemarrhenae sheet, fry according to the saline method of processing and do.The rational technic index of its concocting method neither one, the profit system time, brinish amount, parch time and temperature or the like are not all controlled, and wherein moisten the system time to be approximately 2～3 days, and consuming time longer, technology is loaded down with trivial details relatively, is not suitable for industrialized great production.Therefore, but the Rhizoma Anemarrhenae processing procedure parameter of the practical quantified controlling of formulation, and reasonably prepared slice quality is investigated index, and is imperative.
Summary of the invention
At the requirement of above-mentioned situation and country, the present invention is directed to the technological parameter that each link is concocted in stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution processing, carried out orthogonal optimum seeking, obtained the optimum process condition of preparation stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution.
The object of the present invention is to provide a kind of concocting method of stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution.
The object of the invention realizes by following technical scheme:
1, a kind of concocting method of stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution, it is as follows to it is characterized in that concocting step:
(1) get rhizoma ane marrhenae, remove hair and impurity, clean, decompression profit system is cut into the sheet that thickness is 2～4mm;
(2) 1～5% salt that will be equivalent to Rhizoma Anemarrhenae sheet weight adds the saline that solid salt weight 4-5 water doubly is made into, and adds in the Rhizoma Anemarrhenae sheet and mixes thoroughly, and moistening to saline is exhausted by medical material;
(3) put in the parch container, 110～200 ℃ of parches 4～20 minutes, taking-up cooled,
Decompression profit system method described in the above-mentioned steps (1) is that vacuum adds gentle method for making or decompression cold-maceration.
It was that medical material is put in the hermetic container that above-mentioned vacuum adds gentle method for making, is decompressed to 0.04～0.10mpa, feeds steam, in 40～100 ℃ of insulations 3-8 hour.
Above-mentioned decompression cold-maceration is that medical material is put in the hermetic container, is decompressed to 0.04～0.10mpa, water is injected flood medical material, recovers normal pressure again, merceration 4-9 hour.
Parch container described in the above-mentioned steps (3) is pan formula or and cylinder type medicine roasting machine.
Beneficial effect of the present invention is as follows:
This concocting method is simple, easy operating, the stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution color and luster that makes, moisture is all within the scope of pharmacopeia regulation, compare with traditional concocting method, be fit to big production operation, the more important thing is that the loss of effective ingredient Sarsasapogenin and chimonin is little, improve 11.41% and 18.93% respectively with traditional handicraft comparison content, improved the effect of Rhizoma Anemarrhenae nourishing YIN and clearing away heat, can effectively satisfy clinical needs, improved clinical efficacy the stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution decoction pieces, the process of preparing Chinese medicine for stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution simultaneously provides concrete technological parameter, and carries out the authentication code system for the prepared slices of Chinese crude drugs foundation is provided.
The specific embodiment
Below in conjunction with embodiment the specific embodiment of the present invention is elaborated.
Embodiment 1.
Get rhizoma ane marrhenae, remove hair and impurity, clean, put in the hermetic container that is decompressed to 0.07mpa, 60 ℃ of profit systems of logical steam are after 5 hours, and manual cutting is the decoction pieces of 3mm specification, after 60 ℃ of following dryings, take out, cool, get Rhizoma Anemarrhenae sheet 500g, other gets 15g salt and adds 75ml water and be made into saline, adds in the Rhizoma Anemarrhenae sheet and mixes thoroughly, moistening to saline is exhausted by Rhizoma Anemarrhenae sheet, puts in the cylinder type medicine roasting machine 180 ℃ of parches 8 minutes, taking-up cools, and packs, promptly.
Embodiment 2.
Get rhizoma ane marrhenae, remove hair and impurity, clean, put in the hermetic container that is decompressed to 0.07mpa, 60 ℃ of profit systems of logical steam are after 5 hours, and rotary medicine cutter cutting is the decoction pieces of 3mm specification, after 60 ℃ of following dryings, take out, cool, get Rhizoma Anemarrhenae sheet 10kg, other gets 300g salt and adds 1500ml water and be made into saline, adds in the Rhizoma Anemarrhenae sheet and mixes thoroughly, moistening to saline is exhausted by medical material, in the horizontalization cooking-pot type medicine roasting machine, and 180 ℃ of parches 8 minutes, taking-up cools, and packs, promptly.
Embodiment 3.
Get rhizoma ane marrhenae, remove hair and impurity, clean, put in the hermetic container that is decompressed to 0.05mpa, add 60 ℃ of profit systems of entry after 8 hours, rotary medicine cutter cutting is the decoction pieces of 3mm specification, after 60 ℃ of following dryings, take out, cool, get Rhizoma Anemarrhenae sheet 10kg, other gets 300g salt and adds 1500ml water and be made into saline, adds in the Rhizoma Anemarrhenae sheet and mixes thoroughly, moistening to saline is exhausted by medical material, puts in the cylinder type medicine roasting machine 190 ℃ of parches 6 minutes, taking-up cools, and packs, promptly.
Embodiment 4.
Get rhizoma ane marrhenae, remove hair and impurity, clean, put in the hermetic container that is decompressed to 0.1mpa, add 60 ℃ of profit systems of entry after 8 hours, manual cutting is the decoction pieces of 3mm specification, after 60 ℃ of following dryings, take out, cool, get Rhizoma Anemarrhenae sheet 500g, other gets 15g salt and adds 75ml water and be made into saline, adds in the Rhizoma Anemarrhenae sheet and mixes thoroughly, moistening to saline is exhausted by the Rhizoma Anemarrhenae, puts in the heat pot 160 ℃ of parches 10 minutes, taking-up cools, and packs, promptly.
The comparative test of active constituent content in the different processed products of embodiment 5. Rhizoma Anemarrhenaes
We have compared the living Rhizoma Anemarrhenae, the content of effective ingredient Sarsasapogenin and chimonin in the Rhizoma Anemarrhenae that stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution that traditional method is concocted and method provided by the invention are concocted, the content that is the Rhizoma Anemarrhenae that method provided by the invention concocts is higher, and compared the effect of three's nourishing YIN and clearing away heat, all the effect of the Rhizoma Anemarrhenae of concocting with method provided by the invention is better.Concrete experimental result is as follows:
The content of Sarsasapogenin and chimonin relatively in the different processed products of the Rhizoma Anemarrhenae
The Sarsasapogenin content assaying method: every duplicate samples is got 0.1g (parallel five times), and accurate the title decides, and puts in the tool plug conical flask, the accurate ethanol 25ml that adds claims to decide weight, soaked overnight, supersound process (power 200W, frequency 30kHz) 40 minutes is put cold, claim again to decide weight, supply the weight that subtracts mistake, shake up with ethanol, filter, precision is measured subsequent filtrate 10ml, evaporate to dryness, add water 10ml, reflux 2 hours is taken out, and is chilled to room temperature, jolting limit, limit drip 40% sodium hydroxide solution to solution colour by orange become orange red, extract 2 times with the chloroform jolting, each 30ml merges chloroform liquid, evaporate to dryness, residue adds dissolve with methanol, is transferred in the 10ml measuring bottle, and is diluted to scale, shake up, get 10 μ l and inject C
18In the chromatographic column; Methanol: water (95: 5) is mobile phase; Flow velocity is 1.0ml/min; 30 ℃ of column temperatures; Detect with evaporation light dispersion dispersion, 85 ℃ of drift tube temperatures are measured peak area, calculate content.The results are shown in Table 1
The chimonin content assaying method: every duplicate samples is got 0.1g (parallel five times), accurate claims surely, adds 50% ethanol ultrasonic extraction 3 times, each 30min, each 15ml solvent filters, and volatilizes solvent, with 50% methanol constant volume in the 5ml volumetric flask.Get 10 μ l and inject C
18In the chromatographic column; Water: acetonitrile: 0.85% phosphoric acid (100: 25: 5) is mobile phase; Flow velocity is 0.6ml/min; 30 ℃ of column temperatures; The detection wavelength is 258nm, measures peak area, calculates content.The results are shown in Table 1
The content (n=5) of Sarsasapogenin and chimonin in the different processed products of table 1 Rhizoma Anemarrhenae
| Group | Sarsasapogenin content (%) | Chimonin content (%) |
| Give birth to the new stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution of the Rhizoma Anemarrhenae traditional salt Rhizoma Anemarrhenae | 1.3514 1.3021 1.4507 | 1.5735 1.4396 1.7122 |
The result shows the Rhizoma Anemarrhenae of concocting with method provided by the invention, its Sarsasapogenin and chimonin content are with the living Rhizoma Anemarrhenae of the traditional method process of preparing Chinese medicine and the content height of stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution, content improves 11.41% and 18.93% respectively, and prompting is little by the Rhizoma Anemarrhenae loss of effective components of concocting with method provided by the invention.
The pharmacological testing of the different processed products of embodiment 6. Rhizoma Anemarrhenaes
1, the different processed product nourishing yin effects of the Rhizoma Anemarrhenae relatively
50 of female Kunming mouses, body weight 18～22g is divided into five groups at random, 10 every group, establishes the normal control group; Model group; Give birth to Rhizoma Anemarrhenae group; Traditional salt Rhizoma Anemarrhenae group; New stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution group.Each organizes gastric infusion 1 time every day, continuous six days, first administration the 3rd day, remove every group of subcutaneous injection thyroxine of blank group 0.4mg/ days, and continuous four days, irritated stomach the 7th day, the single volume that places is filling in the 50g sodica calx wide mouthed bottle of 250ml, to cover tightly, and respectively organize the time-to-live with the stopwatch record immediately, data are carried out statistical procedures.The results are shown in Table 2.
The different processed products of table 2 Rhizoma Anemarrhenae to the nourishing yin effect of mice (
N=10)
| Group | Dosage (g/kg) | Time-to-live (min) |
| Blank group model group is given birth to the new stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution group of Rhizoma Anemarrhenae group traditional salt Rhizoma Anemarrhenae group | - - 5 5 5 | 41.9±20.3 24.5±9.5Δ 33.6±9.2** 35.8±5.6** 40.0±10.1** |
Relatively compare * * p＜0.01 with model group in Δ p＜0.05 with the blank group
Hyperthyroidism (hyperthyroidism), it is dialectical to press tcm clinical practice, and the overwhelming majority is the disease of the deficiency of YIN, an important symptom of hyperthyroidism is oxygen consumption to be increased, and hypoxia-bearing capability reduces, and therefore duplicates hyperthyroidism " deficiency of YIN " model with white mice, with the hypoxia-bearing capability is index, observes the nourishing yin effect of the Rhizoma Anemarrhenae.The result shows, with the mice time-to-live be index, relatively there were significant differences for model group and normal saline group, thyroxine modeling success is described, mice has obvious hyperthyroidism symptom, living Rhizoma Anemarrhenae group, traditional salt Rhizoma Anemarrhenae group are compared with model group with new stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution group all significant difference, illustrates that the living Rhizoma Anemarrhenae and stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution all have certain nourishing yin effect, can find out that by numerical value the Rhizoma Anemarrhenae nourishing yin effect of the method process of preparing Chinese medicine provided by the invention is the strongest.
2, the different processed product heat clearing away of Rhizoma Anemarrhenae effect relatively
50 of Wistar rats are divided into five groups at random, 10 every group, establish the normal control group; The thyroxine group; Give birth to Rhizoma Anemarrhenae group; Traditional salt Rhizoma Anemarrhenae group; New stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution group.Each is organized and irritates the corresponding extracting solution of stomach every day 1 time, remove the normal control group in addition, gavage thyroxine 2.5mg every group of every day, experimentizes after three weeks, dissects rat, get liver, mucous membrane of small intestine, the renal tissue piece of constant weight, with cold normal saline rinsing, remove blood, filter paper is dried, weigh, put into small beaker.The normal saline of 9 times of amounts of volume ratio by weight of measuring pre-cooling with pipet is cut for a short time with ophthalmology and to be shredded piece of tissue as early as possible in beaker, grinds up and down with high-shear emulsion machine 10000r/min and makes 10% tissue homogenate.With centrifugal 5 minutes of 1000 rev/mins of low speed centrifuges, stay supernatant to abandon following precipitation centrifugal good homogenate 10% homogenate for preparing.Get supernatant 0.2ml and add the 0.8ml normal saline to be diluted to 2% homogenate to be measured, measure 2% tissue homogenate albumen with Coomassie brilliant blue reagent simultaneously.The results are shown in Table 3
Na in the tissue
+, K
+-ATP enzyme activity=(measuring pipe OD value-control tube OD value) ÷ standard pipe OD value * standard pipe concentration * 2.5 * 6 ÷ homogenate protein concentrations
The different processed products of table 3 Rhizoma Anemarrhenae are to Na in each internal organs of rat
+, K
+-atpase activity influence (x ± s n=10)
| Group | Hepatocyte | Mucous membrane of small intestine | Kidney |
| Normal control group thyroxine group is given birth to Rhizoma Anemarrhenae group traditional salt Rhizoma Anemarrhenae group | 0.57±0.22 1.24±0.20 0.99±0.43 0.72±0.35* | 2.19±1.07 4.65±0.54 2.94±1.49 2.24±0.80* | 9.62±4.03 15.86±2.96 12.08±4.17 9.86±1.63 |
| New stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution group | 0.50±0.32* | 2.40±0.75* | 9.40±4.21 |
Liver is a parenchymal viscera maximum in the body, Na in the liver cell
+, K
+The variation of-ATP enzyme can influence whole heat with cold, and the nourishing YIN to lower pathogenic fire medicine is suppressing Na
+, K
+-atpase activity aspect has certain effect, constipation with dry stool during the soluble interior-heat caused by deficiency of YIN of the variation of this enzyme in kidney and the intestinal mucosa cells simultaneously, oliguria with reddish urine.The result shows that the equal tool of the different processed products of the Rhizoma Anemarrhenae necessarily suppresses Na
+, K
+The effect of-atpase activity.Wherein the stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution made from concocting method provided by the invention suppress in the liver cell and the Na+ of kidney, K+-ATP enzymatic activity on sample than other strong, shown heat clearing away effect preferably.
The used new stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution of above-mentioned pharmacological testing is embodiment 1 gained.
Claims (5)
1. the concocting method of a stir-baked RHIZOMA ANEMARRHENAE before sprinking salt solution, it is as follows to it is characterized in that concocting step:
(1) get rhizoma ane marrhenae, remove hair and impurity, clean, decompression profit system is cut into the sheet that thickness is 2～4mm;
(2) 1～5% salt that will be equivalent to Rhizoma Anemarrhenae sheet weight adds the saline that solid salt weight 4-5 water doubly is made into, and adds in the Rhizoma Anemarrhenae sheet and mixes thoroughly, and moistening to saline is exhausted by medical material;
(3) put in the parch container, 110～200 ℃ of parches 4～20 minutes, taking-up cooled, and packed, promptly.
2. according to the described concocting method of claim 1, it is characterized in that in the profit of the decompression described in the step (1) system method being that vacuum adds gentle method for making or decompression cold-maceration.
3. according to the described concocting method of claim 1, it is characterized in that at the parch container described in the step (3) be pan formula or cylinder type medicine roasting machine.
4. according to the described concocting method of claim 2, it is characterized in that it is that medical material is placed hermetic container that described vacuum adds gentle method for making, be decompressed to 0.04～0.10mpa, feed steam, in 40～100 ℃ of insulations 3-8 hour.
5. according to the described concocting method of claim 2, it is characterized in that described decompression cold-maceration for being that medical material is placed hermetic container, is decompressed to 0.04～0.10mpa, water is injected flood medical material, recover normal pressure again, merceration 4-9 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101920010A CN101229315A (en) | 2007-12-28 | 2007-12-28 | Method for producing salty rhizoma anemarrhenae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNA2007101920010A CN101229315A (en) | 2007-12-28 | 2007-12-28 | Method for producing salty rhizoma anemarrhenae |
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| Publication Number | Publication Date |
|---|---|
| CN101229315A true CN101229315A (en) | 2008-07-30 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CNA2007101920010A Pending CN101229315A (en) | 2007-12-28 | 2007-12-28 | Method for producing salty rhizoma anemarrhenae |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102755492A (en) * | 2012-06-26 | 2012-10-31 | 亳州市长生中药饮片有限公司 | Method for decocting common anemarrhena |
| CN103845475A (en) * | 2012-11-30 | 2014-06-11 | 中国医学科学院药用植物研究所 | Salted tangerine seed processing method |
-
2007
- 2007-12-28 CN CNA2007101920010A patent/CN101229315A/en active Pending
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102755492A (en) * | 2012-06-26 | 2012-10-31 | 亳州市长生中药饮片有限公司 | Method for decocting common anemarrhena |
| CN103845475A (en) * | 2012-11-30 | 2014-06-11 | 中国医学科学院药用植物研究所 | Salted tangerine seed processing method |
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Open date: 20080730 |