CN101209058B - Composite biological insecticide - Google Patents
Composite biological insecticide Download PDFInfo
- Publication number
- CN101209058B CN101209058B CN2006101600018A CN200610160001A CN101209058B CN 101209058 B CN101209058 B CN 101209058B CN 2006101600018 A CN2006101600018 A CN 2006101600018A CN 200610160001 A CN200610160001 A CN 200610160001A CN 101209058 B CN101209058 B CN 101209058B
- Authority
- CN
- China
- Prior art keywords
- enhancin
- bacillus thuringiensis
- anachoreta
- portions
- granulosis virus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a compound living insecticide, including materials of the following volume portions of 10 to 20 portions of diflubenzuron; 0.01 to 0.1 portion of tryptophan; 1 to 2 portions of dispersant; 2 to 5 portions of glycerin; 72.9 to 86.99 portions of sterile water; the invention also includes clostera anachoreta granulosis virus, Bacillus thuringiensis fungus gemma and ENHANCIN increasing protein. The content of the clostera anachoreta granulosis virus is 0.5 to 2 billion per milliliter; the content of the Bacillus thuringiensis fungus gemma is 2 to 6 billion per milliliter; the content of the ENHANCIN increasing protein is 20 to 50 international units per milliliter. The compound living insecticide has the advantages of reasonable compatibility of medicines, convenient manufacture, broad desinsection spectrum and good desinsection effect. The invention overcomes the defects of wettable powder and emulsion; accessory ingredient is easy to disperse. The invention is more convenient to apply in fields and has no remnant and environment pollution, thus having remarkable superiority in actual application and being suitable for massive application on the forest industry.
Description
Technical field
The invention belongs to the biocide that contains virus, relate in particular to a kind of composite biological insecticide at the willow lepidoptera pest.
Background technology
In recent years, the willow defoliator has caused enormous economic loss for China's production of forestry, and they mainly are to be the lepidoptera pest of representative with poplar caterpillar, Yang Xiaozhou moth, Closteraanastomosis L., poplar looper, gypsymoth and willow moth.These insects are widely distributed in China, and generation overlap mixes the generation breeding, and food leaf amount is big, and along with the influence of global warming and other factors, the generation of willow lepidoptera pest and harm have the trend that increases the weight of year by year.Poplar caterpillar in 2005 and Yang Xiaozhou moth in Henan, province many places outbursts such as Hubei, Hunan, make large stretch of poplar forest harm and serious, limited the development of the willow plant husbandry of China greatly.
Present willow control of insect is mainly by chemical pesticide, but the insect reproduction speed is fast, the annual generation many generations, and chemical pesticide also needs frequent the use, and this just makes poplar caterpillar that the number of chemical agricultural chemicals has been produced serious resistance.In addition, mostly being street tree and shelter forest because the condition of a disaster takes place by serious forest, also is the movable frequent areas of people, animal, uses chemical pesticide certainly will cause pollution to environment in a large number, to the harm of health and livestock and poultry.Therefore, the use of minimizing and restriction chemical insecticide is imperative.
Biological insecticides have to host insect good disinsection effect, host specificity height, to the person poultry harmless, do not injure natural enemy, advantage such as free from environmental pollution, and its development and application prospect is quite wide.But it should be noted that its advantage also becomes the chains that limit it and further develop simultaneously.Making people execute a medicine such as, its high host specificity can only kill a kind of or several insects of only a few; It duplicates its biological decision after must be in pest body and just can make insect show symptom, so just greatly reduces the desinsection speed of agricultural chemicals.In order to overcome these defectives, scientists is actively being sought various approach.Usually can solve these problems from three aspects: the first, utilize genetic engineering means to transform microorganism and product thereof, obtain the agricultural chemicals of efficient, wide spectrum, high poison; Its two, in insecticide, add synergist such as fluorescent whitening agent, chitin etc., improve the insecticidal rate of agricultural chemicals; Its three, the development composite biological insecticide enlarges the insecticidal spectrum and the desinsection speed of agricultural chemicals.At present, the relevant expert is more to the research of preceding two kinds of approach, and that composite biological insecticide is many because of research object, the test is complicated, will combine with actual production etc. is former thereby relatively lag behind.
For biological insecticides, viral insecticide and bacillus thuringiensis,Bt are its two big tap, and be the most deep, most widely used general at the biological control area research.Granulosis virus of Ciostera anachoreta Fabricius belongs to Rhabdoviridae, observes under Electronic Speculum, and the virion volume morphing is an oval, its mean size is 337nm * 170nm, and it is shaft-like that virion is, the blunt circle in two ends, size is 250nm * 46nm, and virion is a monokaryon capsid embedding type.Granulosis virus of Ciostera anachoreta Fabricius to 3 ages above target larva pathogenicity very high, when the generation of poplar caterpillar continuous large-area, when insect density is big, the natural infection rate can be up to 40%～60%, is the key factor that the big generation of fan boat moth is raised in control, has good using value.Bacillus thuringiensis,Bt (Bacillusthuringiensis is called for short Bt) be development time the earliest, most widely used class lepidoptera pest biological insecticides.Its mechanism of action is, Bt is the bacterium of a kind of shaft-like, Gram-positive, formation gemma, and ripe gemma contains does not have active lenticular toxic protein.After insect ate, toxalbumin was activated by alkaline intestinal juice, and is degraded by erepsin, formed less D2 endotoxin molecule, destroyed the enterocyte of insect, infiltered haemocoele and caused septicemia, caused insect death.Initial people think that always insect can not produce resistance to Bt.Studies confirm that if use the Bt preparation 10 every year, after continuous 5 years, the medial lethal dose of insect will double.Though said preparation has special effect to this class lepidoptera pest of control poplar caterpillar, thereby is widely used, its resistance will be along with deeply more and more significantly showing of using.
The young urea that goes out is a kind of insect growth regulator, IGR, belongs to specificity insecticide.After insect was got food or contact medicament, it was chitinous synthetic to suppress epidermis, larva can not normally be casted off a skin and death.Mainly be stomach poison function, certain action of contace poison is also arranged, but absorption in not having.Lepidopterous larvae is had special efficacy, do not kill adult, but can make adult sterile, ovum can not normally be hatched.Toxicity is low, to people, animal and plant safety, natural enemy is killed and wounded little, and drug effect is slower, could show insecticidal action after 2～3 days.
Summary of the invention
The object of the present invention is to provide that a kind of compatibility is reasonable, easy to make, insecticidal spectrum is wide, the composite biological insecticide at the willow lepidoptera pest of good disinsection effect.
In order to achieve the above object, the present invention adopts following technical scheme: a kind of composite biological insecticide, the raw material that comprises following parts by volume: young urea 10～20 goes out, tryptophan 0.01～0.1, dispersant 1～2, glycerine 2～5, sterile water 72.9～86.99, also comprise granulosis virus of Ciostera anachoreta Fabricius, bacillus thuringiensis gemma and ENHANCIN strengthen protein Preparation and form, the content of granulosis virus of Ciostera anachoreta Fabricius is every milliliter 5～2,000,000,000 PIB, the content of bacillus thuringiensis gemma is every milliliter 20～6,000,000,000, and the content that ENHANCIN strengthens albumen is every milliliter 20～50 international unit.
Described dispersant is L602, LH656 or L656.
The preparation method of described composite biological insecticide is: at first granulosis virus of Ciostera anachoreta Fabricius, bacillus thuringiensis, the young urea that goes out, ENHANCIN are strengthened albumen and mix, then successively to wherein adding glycerine, tryptophan, dispersant, sterile water, stir, obtain suspending agent and get final product.
Strengthen the protein that albumen (ENHANCIN) is a kind of metalloprotein enzymatic property of mainly finding in the Granulovirus of Rhabdoviridae.This albumen can improve the infectivity of insect viruses and other pathogens effectively, when cabbage looper PuGV (TnGV) synergistic protein of purifying and granulosis virus of Ciostera anachoreta Fabricius are together fed the poplar caterpillar larva, can make the appeal of granulosis virus of Ciostera anachoreta Fabricius improve 12 times; Mix use with Bt, can make the virulence of Bt improve 2-7 doubly.Therefore, strengthening albumen has broad application prospects improving baculoviral insecticidal efficient and widen aspect such as integrated pest control.
The present invention compared with prior art has the following advantages and effect: granulosis virus of Ciostera anachoreta Fabricius can form epidemic disease for a long time in pest population, guaranteed the aftereffect of this insecticide; The bacillus thuringiensis and the young urea that goes out can be killed most willow lepidoptera pests, have possessed the insecticidal spectrum of broad; It is biological potentiating agent that ENHANCIN strengthens albumen, can promote virus and bacterium to the invasion of insect midgut, has guaranteed higher desinsection speed; Four kinds of main components are combined and can be had complementary advantages, and mutually promote, not only can kill lepidoptera pest fast in 2-4 days, and have kept the advantage of permanent, environmentally safe biological insecticides action time.This insecticide is a suspending agent, and it has overcome the defective of wetting powder and emulsifiable concentrate, and auxiliary agent is easy to disperse, and the field is used more convenient, does not have residual and environmental pollution.This has remarkable advantages in actual applications, is suitable for using in a large number in forestry.
Embodiment
Embodiment 1: select 10 milliliters of the young ureas of going out for use, 0.1 milliliter of tryptophan, 2 milliliters of L602 dispersants, 5 milliliters of glycerine, 82.9 milliliters of sterile waters, granulosis virus of Ciostera anachoreta Fabricius 1,000 hundred million PIB, 4,000 hundred million of bacillus thuringiensis gemma and ENHANCIN strengthen albumen 3500 international units.Dispersant is selected L602 for use.At first granulosis virus of Ciostera anachoreta Fabricius, bacillus thuringiensis, the young urea that goes out, ENHANCIN are strengthened albumen and mix, add glycerine, tryptophan, dispersant, sterile water then successively, stir, obtain suspending agent and get final product.
Embodiment 2: select 200 milliliters of the young ureas of going out for use, and 0.5 milliliter of tryptophan, 10 milliliters of LH656 dispersants, 20 milliliters of glycerine, granulosis virus of Ciostera anachoreta Fabricius 5,000 hundred million PIB, bacillus thuringiensis gemma 60,000 hundred million, ENHANCIN strengthen protein 20 000 international unit.At first granulosis virus of Ciostera anachoreta Fabricius, bacillus thuringiensis, the young urea that goes out, ENHANCIN are strengthened albumen and mix, add glycerine, tryptophan, dispersant then successively, add sterile water constant volume to 1 liter at last, stir, obtain suspending agent and get final product.
Embodiment 3: in the present embodiment, select 150 milliliters of the young ureas of going out for use, 0.1 milliliter of tryptophan, 15 milliliters of dispersants, 40 milliliters of glycerine, 794.9 milliliters of sterile waters, granulosis virus of Ciostera anachoreta Fabricius 15,000 hundred million PIB, bacillus thuringiensis gemma 50,000 hundred million and ENHANCIN strengthen protein 20 000 international unit.Dispersant is selected L656 for use.Other is identical with embodiment 1.
Embodiment 4: select 10 milliliters of the young ureas of going out for use, 0.01 milliliter of tryptophan, 1 milliliter of dispersant, dispersant is L602,2 milliliters of glycerine, 86.99 milliliters of sterile waters, granulosis virus of Ciostera anachoreta Fabricius 50,000,000,000 PIB, bacillus thuringiensis gemma 3,000 hundred million and ENHANCIN strengthen albumen 4500 international units.Other is identical with embodiment 1.
Embodiment 5: select 20 milliliters of the young ureas of going out for use, 0.1 milliliter of tryptophan, 2 milliliters of dispersants, dispersant is L656,5 milliliters of glycerine, 72.9 milliliters of sterile waters, granulosis virus of Ciostera anachoreta Fabricius 2,000 hundred million PIB, bacillus thuringiensis gemma 3,500 hundred million and ENHANCIN strengthen albumen 5000 international units.Other is identical with embodiment 1.
Embodiment 6: select 18 milliliters of the young ureas of going out for use, 0.07 milliliter of tryptophan, 1.5 milliliters of dispersants, dispersant is L656,2.5 milliliters of glycerine, granulosis virus of Ciostera anachoreta Fabricius 1,800 hundred million PIB, bacillus thuringiensis gemma 5,500 hundred million, ENHANCIN strengthen protein 20 00 international unit.At first granulosis virus of Ciostera anachoreta Fabricius, bacillus thuringiensis, the young urea that goes out, ENHANCIN are strengthened albumen and mix, add glycerine, tryptophan, dispersant then successively, add sterile water constant volume to 100 milliliter at last, stir, obtain suspending agent and get final product.
Embodiment 7: select 12 milliliters of the young ureas of going out for use, 0.08 milliliter of tryptophan, 1.3 milliliters of dispersants, dispersant is L656,4.5 milliliters of glycerine, granulosis virus of Ciostera anachoreta Fabricius 2,000 hundred million PIB, bacillus thuringiensis gemma 4,000 hundred million, ENHANCIN strengthen albumen 5000 international units.At first granulosis virus of Ciostera anachoreta Fabricius, bacillus thuringiensis, the young urea that goes out, ENHANCIN are strengthened albumen and mix, add glycerine, tryptophan, dispersant then successively, add sterile water constant volume to 100 milliliter at last, stir, obtain suspending agent and get final product.
Claims (3)
1. composite biological insecticide, it is characterized in that: the raw material that comprises following parts by volume: young urea 10～20 goes out, tryptophan 0.01～0.1, dispersant 1～2, glycerine 2～5, sterile water 72.9～86.99, comprise that also granulosis virus of Ciostera anachoreta Fabricius, bacillus thuringiensis gemma and ENHANCIN strengthen protein Preparation and form, the content of granulosis virus of Ciostera anachoreta Fabricius is every milliliter 5～2,000,000,000 PIB, the content of bacillus thuringiensis gemma is every milliliter 20～6,000,000,000, and the content that ENHANCIN strengthens albumen is every milliliter 20～50 international unit.
2. composite biological insecticide as claimed in claim 1 is characterized in that: described dispersant is L602, LH656 or L656.
3. the preparation method of composite biological insecticide as claimed in claim 1 or 2, it is characterized in that: at first granulosis virus of Ciostera anachoreta Fabricius, bacillus thuringiensis, the young urea that goes out, ENHANCIN are strengthened albumen and mix, then successively to wherein adding glycerine, tryptophan, dispersant, sterile water, stir, obtain suspending agent and get final product.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101600018A CN101209058B (en) | 2006-12-29 | 2006-12-29 | Composite biological insecticide |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2006101600018A CN101209058B (en) | 2006-12-29 | 2006-12-29 | Composite biological insecticide |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN101209058A CN101209058A (en) | 2008-07-02 |
| CN101209058B true CN101209058B (en) | 2011-09-07 |
Family
ID=39609451
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2006101600018A Expired - Fee Related CN101209058B (en) | 2006-12-29 | 2006-12-29 | Composite biological insecticide |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN101209058B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104542710A (en) * | 2014-12-03 | 2015-04-29 | 江西省新龙生物科技有限公司 | Virus insecticidal suspension agent containing insect hormone insecticide |
| CN107709275B (en) | 2015-06-02 | 2019-01-11 | 科氏农艺服务有限责任公司 | Microbial bacterial agent composition and its purposes in agricultural |
| CN106577764A (en) * | 2016-11-11 | 2017-04-26 | 河南农业大学 | Mamestra brassicae nuclear polyhedrosis virus-containing pesticidal suspending agent and preparation method thereof |
| CN112075423A (en) * | 2020-09-25 | 2020-12-15 | 石家庄农悦生物科技有限公司 | Underground pest inhibitor and preparation method and application thereof |
-
2006
- 2006-12-29 CN CN2006101600018A patent/CN101209058B/en not_active Expired - Fee Related
Non-Patent Citations (1)
| Title |
|---|
| 袁哲明等.重组增效蛋白对Bt和氯氰菊酯防治棉铃虫的增效作用.《中国生物防治》.2006,第22卷(第3期),194-197. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN101209058A (en) | 2008-07-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US9320771B2 (en) | TTO-based wide spectrum therapeutics, disinfectants and anesthetics for use in aquaculture | |
| CN101209058B (en) | Composite biological insecticide | |
| CN101317572B (en) | Mixed plant source insecticide | |
| CN103283767A (en) | Spirotetramat-containing pesticidal composition | |
| CN103783030A (en) | Method of controlling pests by combination of insect predator Carabussmaragdinus Fischer and Metarrhiziumanisopliae | |
| CN109221183A (en) | A kind of Pesticidal combination of ethyoxyl modified poly- trisiloxanes and biogenic pesticide | |
| CN107197861A (en) | A kind of CPV and the insecticidal suspending agent of muscardine and preparation method thereof | |
| CN103053551A (en) | Spinosad-containing insecticidal composition | |
| CN101874501B (en) | Insecticidal composition containing thiamethoxam and carbosulfan | |
| CN101796962B (en) | Insecticidal composition containing clothianidin and malathion | |
| JP4052535B2 (en) | Animal drugs and animal feed | |
| CN107320481A (en) | Application of the tannic acid in killing and preventing aquatic livestock vermin | |
| CN104430630A (en) | Agricultural medicine adopting plants as raw materials | |
| CN101019546A (en) | Recompounded pesticide of emamectin benzoate and fipronil | |
| CN103300021A (en) | Chromafenozide-biologically sourced pesticide composition | |
| CN105343879A (en) | Nematode-trapping fungus and vermifuge combined medication box and its application | |
| KR101850697B1 (en) | Composition for preventing or treating scuticociliatosis in fishes, and natural feed having the same | |
| CN101897341A (en) | Insecticidal composition containing thiamethoxam and malathion | |
| CN106234370A (en) | A kind of containing benzo alkene fluorine bacterium azoles and the bactericidal composition of osthole | |
| CN106070260A (en) | A kind of containing avilamycin with the composition pesticide of CGA-173506 | |
| CN101878783A (en) | Compound preparation for killing ticks and preparation method thereof | |
| CN101897337B (en) | Microbial pesticide synergist and microbial pesticide containing same | |
| CN110122503A (en) | A kind of insecticide containing effective cypermethrin and lufenuron | |
| CN106259379B (en) | A kind of nematicidal composition containing trifluorobutene base class compound | |
| RU2433589C1 (en) | Method to control larvae of bloodsucking insects |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20110907 Termination date: 20131229 |