CN101199465A - Collagen and production promoter for hyaluronic acid - Google Patents
Collagen and production promoter for hyaluronic acid Download PDFInfo
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- CN101199465A CN101199465A CNA2007101679927A CN200710167992A CN101199465A CN 101199465 A CN101199465 A CN 101199465A CN A2007101679927 A CNA2007101679927 A CN A2007101679927A CN 200710167992 A CN200710167992 A CN 200710167992A CN 101199465 A CN101199465 A CN 101199465A
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Abstract
The invention relates to a medicament which has an anxo-action for producing collagen or/and hyaluronic acid (collagen or/and hyaluronic acid producing accelerant), skin external agent or food which also has the anxo-action for producing collagen or/and hyaluronic acid. At least one sort of pollen, pollinarium and extractive of both is selected to use as the active ingredient of the collagen or/and hyaluronic acid producing accelerant. In addition, the roduced depressor is used as the active ingredient of the skin external agent, collagen or/and hyaluronic acid producing accelerant food.
Description
Technical field
The present invention relates to collagen and/or hyaluronic generation promoter.And the present invention relates to contain the skin preparations for extenal use and the food of above-mentioned generation promoter.
Background technology
Because various pollen such as willow shirt, artemisiifolia become the cause of pollinosis, think that at present pollen is allergen.And, because the pollen stimulating immune system, so also studying pollen to immune pharmacological action.
Disclose the culture that pollen is obtained by fermentations such as lactobacillus, bacillus bifidus or yeast in the patent documentation 1 and had skin regeneration effect, crease-resistant effect etc., it has been mixed in pharmaceuticals, the cosmetics.
The oil body that can obtain from Pollen Brassicae campestris is disclosed in the patent documentation 2.
Disclosing in the patent documentation 3 with the pollen of herbage, corn, hazel, rye (Secale cereale L.), Salix gracilistyla, Cortex Populi dividianae, French chrysanthemum or pinaster is the pollen extract soft capsule of raw material, and this capsule has the inhibition effect to prostatitis, hyperplasia of prostate.
Patent documentation 1: Japanese Unexamined Patent Application Publication 2001-504510 communique
Patent documentation 2: Japanese Unexamined Patent Application Publication 2002-503268 communique
Patent documentation 3: Japanese kokai publication hei 9-278665 communique
Non-patent literature 1: " bee science " 7 (1): 9-12 Honeybee Science (1986)
Summary of the invention
The objective of the invention is to,, provide their new purposes based at pollen, pollen load (pollen lotus) or their the newfound physiologically active of extract.Particularly, its purpose is, provides as collagen or/and the purposes of hyaluronic generation promoter and the cosmetic or the food that contain this generation promoter.
The inventor studies for the physiological activity of pollen, pollen load or their extract, found that extract at the pollen load of frostweed, tea, Brassica campestris L and Semen Fagopyri Esculenti, particularly in the extract of water or pure isopolarity solvent, have former generation facilitation of strong rubber or hyaluronic acid and produce facilitation.
The present invention is found to be the basis and finishes with above-mentioned, provides following collagen production promoter, hyaluronic acid to produce promoter, skin preparations for extenal use and food.
I. collagen is or/and hyaluronic generation promoter
(I-1) the generation promoter of at least one in a kind of collagen or the hyaluronic acid is characterized in that, comprises to be selected from least a in the group of being made up of pollen, pollen load and their extract.
(I-2) according to (I-1) described generation promoter, wherein, above-mentioned pollen or pollen load are from belonging to the plant that is selected from least one section in the group of being made up of Cruciferae, Polygonaceae, Theaceae and Cistaceae.
(I-3) according to (I-2) described generation promoter, wherein, above-mentioned plant is to be selected from least a in the group of being made up of frostweed, tea, Brassica campestris L and Semen Fagopyri Esculenti.
(I-4) according to each described generation promoter in (I-1)～(I-3), wherein, the said extracted thing is the extract that is selected from water, ethanol or their mixture of the pollen of at least a plant in the group of being made up of frostweed, tea, Brassica campestris L and Semen Fagopyri Esculenti or pollen load.
(I-5) a kind of collagen production promoter is characterized in that, comprises the pollen that is selected from least a plant in the group of being made up of frostweed, tea and Brassica campestris L or pollen load or any extract in them.
(I-6) a kind of hyaluronic acid promoter is characterized in that, comprises the pollen that is selected from least a plant in the group of being made up of Brassica campestris L and Semen Fagopyri Esculenti or pollen load or any extract in them.
II. skin preparations for extenal use
(II-1) a kind of skin preparations for extenal use has wherein mixed each described generation inhibitor in above-mentioned (I-1)～(I-4).
In addition, this skin preparations for extenal use also can be as described below.
(II-a) a kind of skin preparations for extenal use is characterized in that, comprises to be selected from least a in the group of being made up of pollen, pollen load and their extract.
(II-b) according to (II-a) described skin preparations for extenal use, wherein, above-mentioned pollen or pollen load are from belonging to the plant that is selected from least one section in the group of being made up of Cruciferae, Polygonaceae, Theaceae and Cistaceae.
(II-c) according to (II-b) described skin preparations for extenal use, wherein, above-mentioned plant is to be selected from least a in the group of being made up of frostweed, tea, Brassica campestris L and Semen Fagopyri Esculenti.
(II-d) according to each described skin preparations for extenal use in (II-a)～(II-c), wherein, the said extracted thing is the extract that is selected from water, ethanol or their mixture of the pollen of at least a plant in the group of being made up of frostweed, tea, Brassica campestris L and Semen Fagopyri Esculenti or pollen load.
III. food
(III-1) a kind of food has wherein mixed the generation inhibitor that contains above-mentioned (I-4) described extract.
In addition, this food also can be as described below.
(III-a) a kind of food is characterized in that, comprises the extract of pollen or pollen load.
(III-b) according to (III-a) described food, wherein, above-mentioned pollen or pollen load are from belonging to the plant that is selected from least one section in the group of being made up of Cruciferae, Polygonaceae, Theaceae and Cistaceae.
(III-c) according to (III-b) described food, wherein, above-mentioned plant is to be selected from least a in the group of being made up of frostweed, tea, Brassica campestris L and Semen Fagopyri Esculenti.
(III-d) according to each described food in (III-a)～(III-c), wherein, the said extracted thing is the extract that is selected from water, ethanol or their mixture of the pollen of at least a plant in the group of being made up of frostweed, tea, Brassica campestris L and Semen Fagopyri Esculenti or pollen load.
Aspect at least a generation of any of these food in being specifically designed to promotion collagen or hyaluronic acid, can distinguish mutually with common food.Particularly contain the food that Brassica campestris L etc. belongs to the extract of the pollen of plant of Cruciferae or pollen load, aspect the generation that is used to promote collagen and hyaluronic acid, can distinguish mutually with common food.
The invention effect
Test shown in the example as described later, because pollen, pollen load or their extract increase collagen and hyaluronic generation, have the skin of giving tension force and moisten, preserve moisture, beautify skin, aging resistance (improving wrinkle etc.), (the improving the skin dimness) effect of etc.ing of whitening, can clearly learn the use of its raw material that can be used as skin preparations for extenal use (particularly cosmetic) and food.Particularly the water extract of rape flower powder ball is simultaneously powerful promotes collagen and hyaluronic generation, therefore as the effective ingredient of skin preparations for extenal use (particularly cosmetic), or very good as raw-food material.
The specific embodiment
(I) collagen is or/and hyaluronic generation promoter
Collagen of the present invention or/and hyaluronic generation promoter be characterised in that, with pollen, pollen load or their extract as effective ingredient.
Wherein, to be Apis solidify pollen and obtain with honey pollen load, is also referred to as pollen dumpling (pollen だ ん ご).This pollen load and Mel together are stored in the honeycomb.This pollen load is easy to reclaim.
Among the present invention, can use any in pollen and the pollen load, but, preferably use pollen load owing to above-mentionedly conveniently obtain.And pollen and pollen load not only can use separately separately, also can mix both and use.
Or/and the source of pollen load (following only be designated as " pollen/pollen load "), section's name of preferred plant, belong to kind of a name, Chinese name (title) shown in following table 1～table 3 as pollen of the present invention.
[table 1]
| Section | Belong to and planting | Chinese name |
| Rubiaceae (Rubiaceae) | Gardenia jasminoides | Fructus Gardeniae |
| Coffea arabica L. C.benghalensis Roxb C.canephora Pierr ex Froeh C.congensis Froeh C.liberica Bull ex Hiem. C.stenophylla G.Don | Coffee | |
| Oenotheraceae (Oenotheraceae (Onagraceae)) | Oenothera biennis | Radix Oenotherae erythrosepalae |
| Epilobium angustifolium L. | Willow herb | |
| Cruciferae (Cruciferae) | Matthiola incana R.Br. | Violet |
| Brassica campestris L. | Brassica campestris L | |
| Orychophragmus violaceus | Eryuelan | |
| Urticaceae (Uruticaceae) | Urtica thunbergiana | Sting the people Herba Urticae Cannabinae |
| Araliaceae (Araliaceae) | Panax Ginseng | Radix Ginseng |
| Fatsia japonica Decne.et Planch. | Rhizoma et Radix Dysosmae Veitchii | |
| Cucurbitaceae (Cucurbitaceae) | Cucurbita pepo | Cucurbita pepo L. |
| Cucumis sativus L. | Fructus Cucumidis sativi | |
| Cucumis melo var.conomon | Snake melon | |
| Citrullus lanatus(＝C.vulgaris) | Citrullus vulgaris | |
| Momordica charantia | Fructus Momordicae charantiae | |
| Sechim edule | Fructus Sechii edulis | |
| Lagenaria siceraria var.gourda | Calabash | |
| Luffa aegyptiaca | The water melon | |
| Lagenaria siceraria var.hispida | Calabash courd | |
| Anacardiaceae (Anacardiaceae) | Rhus succedanea L. | The haze tallow tree |
| Mangifera indica L. | Fructus Mangifera Indicae | |
| Ebenaceae (Ebenaceae) | Diospyros kaki Thunb. | Fructus Kaki |
| Betula platyphylla var.japonica | Betula platyphylla Suk. | |
| Compositae (Compositae) | Cirsium | Ji |
| Cosmos bipinnatus Cav. | Coreopsis | |
| Taraxacum platycarpum Dahlst. | Herba Taraxaci | |
| Farfugium japonicum Kitam. | Coniogramme japonica (Thunb.) Diels | |
| Helianthus annuus L. | Helianthi | |
| Carthamus tinctorius | Flos Carthami | |
| Centaurea cyanus | Centaurea cyanus | |
| Rhamnaceae (Rhamnaceae) | Ziziphus jujube | Fructus Jujubae |
| Scrophulariaceae (Scrophulariaceae) | Veronica persica Poir. | The Persian Herba veronicae |
| Labiatae (Labiatae) | Salvia splendens S.officinalis S.verbenacea | Salvia japonica Thunb. |
| Perilla frutescens | Folium Perillae | |
| Salvia officinalis | Fructus Ananadis comosi Mus grass | |
| Mentha x piperita | Herba Menthae | |
| Lavandula angustifolia Mill. | Lavandula angustifolia |
[table 2]
| Section | Belong to and planting | Chinese name |
| Labiatae (Labiatae) | Rosmarinus officinalis | Herba Rosmarini Officinalis |
| Thymus serpyllum | Herba thymi vulgaris | |
| Tiliaceae (Tiliceae) | Tilia maximowicziana Shirasawa | The Liao Dynasty Hibiscus syriacus |
| Tilia japonica Simk. | Bodhi tree | |
| Tilia miqueliana Maxim. | Tilia miqueliana | |
| Caprifoliaceae (Caprifoliaceae) | Lonicera japonica | Flos Lonicerae |
| Weigela coraeensis Thunb. | Weigela coraeensis | |
| Violaceae (Violaceae) | Viola mandshurica W.Becker | Common violet |
| Meliaceae (Meliaceae) | Melia azedarach | Chinaberry |
| Polygonaceae (Polygonaceae) | Fagopyrum esculerrtum Moench | Semen Fagopyri Esculenti |
| Ericaceae (Ericaceae) | V.angustifolium Ait. V.corymbosum L. V.australe Small V.ashei Reade | Blue berry |
| Theaceae (Theaceae) | Camellia sasanqua Thunb. | Camellia sasanqua Thunb. |
| Camellia sinensis(L.)O.Kuntze | Tea | |
| Stewartia pseudo-camellia | Summer Chinese toon | |
| Camellia japonica L. | Flos Camelliae Japonicae | |
| Hippocastanaceae (Hippocastanaceae) | Aesculus turbinata Blume | Aesculus chinensis Bunge |
| Aesculus hippocastanum | Aesculus hippocastanum L. | |
| Rosaceae (Rosaceae) | Pruns armeniaca | Fructus Pruni |
| Fragaria ananassa Duch. | Fructus Fragariae Ananssae | |
| Prunus mume Sieb.Et Zucc. | Prunus mume (sieb.) sieb.et zucc. | |
| Prunus x yedoensis | Cherry | |
| Crataegus cuneata | Fructus Crataegi | |
| Pyrus pyrifolia var.culta Rehder | Japanese pear | |
| Pyrus ussuriensis Mayim var. sinensis Kikuchi | China's pears | |
| Pyrus communis L.var.sativa DC. | European pear | |
| Prunus zippeliana Miq. | The Da Ye cherry | |
| Eriobotrya japonica Lindl. | Semen Ricini | |
| Rubus fruticosus | Blackberry | |
| Prunus persica Batsch | Peach | |
| Rubus idaeus | Fructus Rubi | |
| Malus pumila var.domestica | Fructus Mali pumilae | |
| Cistaceae (Cistaceae) | Cistus X purpureus(C.creticus X C.ladanifer) | Frostweed |
| Mang cattle Seedling section (Geraniaceae) | Geranium nepalense Sweet | Nepal cranesbill |
| Vitaceae (Vitaceae) | Vitis spp. | Fructus Vitis viniferae |
| Cayratia japonica(Thunb.)Gagnep. | Crow liana prunus mume (sieb.) sieb.et zucc. | |
| Myrtaceae (Myrtaceae) | Leptospermum scoparium | Leptospermum scoparium |
| Eucalyptus | Eucalyptus globulus | |
| Eucalyptus marginata | Eucalyptus eamalducensis Dehnd | |
| Eucalyptus camaldulensis | The American Red Eucalyptus | |
| Fagaceae (Fagaceae) | Castanea crenata Sieb.Et Zucc. | Japanese chestnut |
| Actinidiaceae (Actinidiaceae) | Actinidia chinensis Planch. | Fructus actinidiae chinensis |
[table 3]
| Section | Belong to and planting | Chinese name |
| Pulse family (Leguminosae) | Acacia | Red Chinese scholartree |
| Medicago sativa L. | Alfalfa | |
| Pisum sativum | Semen Pisi sativi | |
| Pueraria lobata(Willd.)Ohwi | Pueraria lobota | |
| Trifolium pratense L. T.repens L. T.hybridum L. | Herba Trifolii Pratentis | |
| Robinia pseudo-Acacia L. | Robinia pseudoacacia L. | |
| Astragalus sinicus L. | Herba Astragali Melilotoidis (Herba Astragali Sinici) | |
| Rutaceae (Rutaceae) | Citrus sinensis Osbeck | Fructus Citri sinensis |
| Evodia danielli Hemsl. | Evodia daneillii (Benn) Hemsl | |
| Zanthoxylum ailanthoides | Fructus Zanthoxyli Ailanthoidis | |
| Phellodendron amurense Ruprecht | Cortex Phellodendri | |
| Cirus aurantium L. | Citrus aurantium Linn. | |
| Citrus unshiu Marc. | Satsuma mandarin | |
| Citrus limon Burm.f. | Fructus Citri Limoniae | |
| Citrus paradisi Macf. | Grapefruit | |
| Citrus kinokuni Hort.ex Tanaka | The money Fructus Citri tangerinae | |
| Citrus tachibana Tanaka | Upright flower Fructus Citri tangerinae | |
| Poncirus trifoliata Raf. | Poncirus trifoliata Raf. | |
| Thousand Qu Lai sections (Lythraceae) | Lagerstroemia indica L. | Lagerstroemia indica L. |
| Sapindaceae (Sapindaceae) | Sapindus mukorossi Gaertn. | Fructus Sapindi Mukouossi |
| Litchi chinensis | Fructus Litchi | |
| Euphonia longana Lam. | Arillus Longan | |
| Cistus section (Oleaceae) | Ligustrum japonicum Thunb. | Ligustrum japonicum Thunb. |
| Magnoliaceae (Magnoliaceae) | Liriodendron tulipifera L. | Liriodendron |
| Aquifoliaceae (Aquifoliaceae) | Ilex rotunda Thunb. | Ilex rotunda Thunb. |
| Ilex pedunculosa | Tool handle Ilicis Purpureae | |
| Salicaceae (Salicaceae) | Salix gracilistyla | Salix gracilistyla |
| Salix | Willow | |
| Salix bakko | The leopard cat willow | |
| Gentianaceae (Gentianaceae) | Swertia japonica | Japan's Herba Swertiae bimaculatae |
As the preferred plant of employed pollen/pollen load origin among the present invention, can enumerate Cruciferae, Polygonaceae, Theaceae and Cistaceae.Specifically,, preferably enumerate violet, Brassica campestris L and eryuelan, preferred especially Brassica campestris L as crucifer.As polygonaceae plant, preferably enumerate Semen Fagopyri Esculenti.As the Theaceae plant, preferably enumerate Camellia sasanqua Thunb., tea, summer Chinese toon and Flos Camelliae Japonicae, preferred especially tea.As the Cistaceae plant, preferably enumerate frostweed.
And, as the effective ingredient of collagen production promoter, can preferably enumerate Cistaceae phytolith Flos Rosae Multiflorae, Theaceae tea plant and crucifer Brassica campestris L.In addition, produce the effective ingredient of promoter, can preferably enumerate crucifer Brassica campestris L and polygonaceae plant Semen Fagopyri Esculenti as hyaluronic acid.
Pollen/pollen load can use with its former form, also can be prepared into the form use of extract with appropriate solvent.
Wherein, the extraction of pollen/pollen load can be undertaken by water, aqueous solvent, organic solvent equal solvent.Specifically, can enumerate water, lower alcohol (methanol, ethanol, normal propyl alcohol, isopropyl alcohol etc.), ketone (acetone, butanone, methyl butyl ketone etc.), esters (methyl acetate, ethyl acetate etc.), ethers (dimethyl ether, Di Iso Propyl Ether etc.), aromatic hydrocarbon (toluene, dimethylbenzene etc.), halogenated hydrocarbons (dichloromethane, chloroform etc.), glycols (ethylene glycol, propylene glycol), glycol ethers (methyl cellosolve, 1,2-dimethoxy-ethane etc.), glycerol, oxolane, acetonitrile, acetic acid, propanoic acid, acetamide, dimethyl formamide, dimethyl sulfoxide.In addition, extract solvent and can use a kind of in these, also can use the mixed liquor of combination in any more than 2 kinds.Mixed liquor can preferably be enumerated the aqueous alcohol of water-containing organic solvent, particularly aquiferous ethanol, aqueous methanol and moisture propanol etc.As extracting solvent, preferred water, ethanol and their mixed liquor (aquiferous ethanol).
Extraction can easily followingly be carried out: pollen/pollen load is added in the solvent, at 0 ℃ of boiling temperature to solvent, be generally under the temperature below 100 ℃, extracted the removal insoluble matter to about 24 hours dipping through about 30 minutes.
The extracting solution of the above-mentioned pollen/pollen load among the present invention, can be directly as collagen production promoter or/and hyaluronic acid produces promoter use, but also can use as required, the dry product of the lyophilization thing, spray drying thing etc. of concentrated extracting solution gained liquid (concentrated solution) or extracting solution.
In order to remove color, taste or stink, as required, also can decolour, shelter or processing such as deodorization the above-mentioned extract that obtains.As such processing, can enumerate the adsorption treatment of utilizing activated carbon etc., the purification process of utilizing column chromatography and ion exchange chromatography etc.
In the extract of the pollen load of each kind of plant (table 1～3), the extract (preferred water extract) of the extract (preferred water or ethanol extraction) of the pollen load of the extract of the pollen load of Cistaceae phytolith Flos Rosae Multiflorae (preferred water or ethanol extraction), Theaceae tea plant and the pollen load of crucifer Brassica campestris L has good collagen and produces facilitation, and the effective ingredient that can be suitable as collagen production promoter uses.
In addition, in the extract of the pollen load of each kind of plant (table 1～3), the extract (preferred alcohol extract) of the pollen load of pollen load extract of crucifer Brassica campestris L (preferred water extract) and polygonaceae plant Semen Fagopyri Esculenti has good hyaluronic acid and produces facilitation, can be suitable as the effective ingredient use that hyaluronic acid produces promoter.
At collagen production promoter of the present invention or/and hyaluronic acid produce in the promoter, the combined amount of pollen/pollen load or their extract, in dry thing, can enumerate 0.0001～100 weight %, preferred 0.001～50 weight %, more preferably 0.01～25 weight %.
Collagen production promoter of the present invention is or/and hyaluronic acid produces promoter, can be preferably as the additive of cosmetics, perhaps as having collagen or/and the beauty treatment that hyaluronic acid produces facilitation with or can ameliorate osteoarthritis, the medical or functional food of symptoms such as gingivitis, rheumatism uses.
Hyaluronic acid as object of the present invention is by the synthetic acid mucopolysaccharide of the hyaluronic acid synthetase in the film that is present in dermal fibroblast (HAS) (J.Invest.Dermatol. such as Y.Sugiyama, 110,116 (1998)).Hyaluronic acid help the moisture of intercellular substance keep, based on the lubricity of keeping, organizing and the flexibility (BIOINDUSTRY, Vol 8,346 (1991)) of gluey substrate formed cell in the tissue.Disclose hyaluronic acid and increased and to reduce Aging such as (, 1,47 (1989)) K.Matsuoka with the age, or degeneration (J.Invest.Dermatol. such as L.A.M.Meyer, 102,385 (1994)), such variation is to cause xerosis cutis, wrinkle, lax an etc. reason.
In addition, the collagen as object of the present invention is the important component that constitutes dermal matrix.Tropocollagen molecule by fibroblast synthetic and be secreted into the extracellular after, rule is crosslinked, keeps the hardness of connective tissue, gives skin tension and elasticity.Disclose skin owing to be exposed in the ultraviolet all the year round, the collagen on corium upper strata reduces J.Invest.Dermatol. such as (, 84,272 (1985)) L.H.Kligman.The collagen fiber degeneration of corium Abstract such as (, 20th IFSCC Congress Cannus, 1,117 (1998)) Y.Nishimori in addition, is also disclosed because uv light exposure.
(2) skin preparations for extenal use, preferred cosmetic
Contain collagen production promoter of the present invention or/and hyaluronic acid produces the skin preparations for extenal use of promoter, collagen based on this collagen production promoter produces facilitation or/and hyaluronic acid produces the hyaluronic acid facilitation of promoter, have good collagen to produce facilitation or/and hyaluronic acid produces facilitation to people's skin respectively, thus can give skin tension force and moisten, prevent and treat skin ageing, eliminate or alleviate wrinkle, make skin water tender, make speckle, dimly show, have the effect of beautifying skin, can keep healthy skin.Therefore, this skin preparations for extenal use, particularly as skin-whitening cosmetics, aging resistance cosmetic, beautify skin cosmetic or skin nursing cosmetic etc. cosmetic useful.So, in the said skin preparations for extenal use of the present invention, comprise purposes as these cosmetics.
The combined amount of pollen/pollen load in this skin preparations for extenal use or their extract in the skin preparations for extenal use total amount, is counted 0.0005～20 weight % with dry thing, preferred 0.001～10 weight %.If it is a lot of to be lower than 0.0005 weight %, the tendency that has effect of the present invention not give full play to if it is a lot of to surpass 20 weight %, then has the tendency of preparation change difficulty.In addition, preferred especially pollen/pollen load or their extract cooperate with the concentration of 0.01～about 1 weight % and are applied to skin.
In the skin preparations for extenal use of the present invention, except pollen/pollen load or their extract, can suitably mix as required and be generally used for skin preparations for extenal use, the composition of cosmetics particularly, for example, whitening agent, wetting agent, antioxidant, oiliness composition, UV absorbent, surfactant, thickening agent, alcohols, powder composition, pigment, water composition, water, various skin-nourishing agent etc.
The dosage form of skin preparations for extenal use of the present invention can adopt dosage form widely such as aqueous solution type, solubilising type, oil-in-water type, powder-type, fluid type, gel-type, ointment type, aerosol-type, water-oily 2 facies patterns and water-oil-powder 3 facies patterns.Be under the situation of basic cosmetics for example,, can enumerate cleansing milk, astringent, emulsion, unguentum, gel, quintessence oil, beautifying liquid, Pack (パ Star Network), facial film etc. as its form at skin preparations for extenal use.In addition, skin preparations for extenal use be face's cosmetic cosmetics (メ one Network ア Star プ) also can, its form can be enumerated foundation cream, lipstick, rouge, eye shadow, eyeliner, mascara etc.Other form can enumerate cleansing milk, massage with agent, clean skin with agent, Hu Houshui, the water that shaves, shaving cream, bath gel, soap, shampoo, hair care element, hair conditioner, typing cream, hair oil, hair growth agent, antiperspirant, hygienic article etc.
(3) food
Being characterized as of food of the present invention, mix having of being put down in writing in (1) collagen produce facilitation or/and the extract of pollen/pollen load that hyaluronic acid produces facilitation as collagen production promoter or/and hyaluronic acid produces promoter.Therefore, in the food of the present invention, based on their effect, having comprised with whiten, beautify skin, preserve moisture, aging resistance etc. is that the beauty treatment of purpose is the food that purpose is used with the diet product or with the symptom that reduces the hypofunction disease (for example arthritis, gingivitis, gum tooth ulcer, rheumatism etc.) that causes owing to collagen/hyaluronic acid that alleviates joint, gingiva etc.
In this food, specifically, can enumerate potus class (drink), beverage class (refreshment drink, milk beverage, lactobacillus beverage, boruga, soda pop, drinks etc.), tablespread, beans, cake, Japanese cake, ice cream, tinned food, instant food, bottled and canned food, gel-like foodstuff, flavouring agent, meat soup, bee product (Mel, Lac regis apis, propolis etc.), milk product, processed fruit, vegetable for processing, the frumentum processed food, souse, the souse raw material, fish product, the poultry meat products, rare delicacies, salted Brassicae siccus, prepared food, frozen food, fatty foods etc.In addition, food of the present invention also can for, add, mixing as collagen production promoter or/and hyaluronic acid produce and can make behind the extract of pollen/pollen load described in (1) of promoter, so-called health food, functional food, dietary supplement, additive, specific health food, patient's food and patient's combined food (MHLW, a kind of food of specific use) or old people's food (MHLW, a kind of food of specific use) form, in this case, can be uncoated tablets, Film coated tablets, coated tablet, granule, powder, tablet, capsule (hard capsule and soft capsule include), masticatory pattern, the syrup type, potus type etc.The extract that adds and mix pollen/pollen load involved in the present invention can use known method to carry out as collagen production promoter or/and hyaluronic acid produces the preparation of the food of promoter.
Embodiment
Below, utilize embodiment, test example and Formulation Example that the present invention is elaborated, but the invention is not restricted to these embodiment.
Embodiment 1: the preparation of the ethanol extraction of pollen load
Add 30ml ethanol in each 10 gram of pollen load (frostweed, tea, Brassica campestris L, Semen Fagopyri Esculenti), mortar grinds, and moves into other containers, and mixes 16～20 hours in the room temperature vibration.Rotating speed is centrifugalize 5 minutes under the 1500rpm, obtains supernatant and precipitation.In the gained precipitate, add the ethanol of 2 times of amounts, grind with mortar once more, move into other containers, and mixed 2 hours in the room temperature vibration.Rotating speed is centrifugalize 5 minutes under the 1500rpm, obtains supernatant.This and the supernatant that obtains are before merged, use rotary evaporation machine (EYELA system) to concentrate, obtain the ethanol extraction of the pollen load of each plant (frostweed, tea, Brassica campestris L, Semen Fagopyri Esculenti).
Embodiment 2: the preparation of the water extract of pollen load
In the resulting final precipitate of the ethanol extraction of embodiment 1, add the water of 3 times of amounts, grind, and at room temperature vibrated 16～20 hours.Then, be centrifugalize 10 minutes under the 2000rpm at rotating speed, and lyophilization gained supernatant.With its water extract, be used for following test example as the pollen load of each plant (frostweed, tea, Brassica campestris L, Semen Fagopyri Esculenti).
The test example
In embodiment 1 and 2 resulting extracts, the water extract of the water extract of the water extract of the frostweed pollen load of the extract of fully being measured in the experiment and ethanol extraction, Flos Camelliae Japonicae powder ball and ethanol extraction and rape flower powder ball, carry out collagen and produced the promotion test, in addition, carry out hyaluronic acid generation promotion test for the water extract of rape flower powder ball and the ethanol extraction of Buckwheat Flower powder ball.
Test example 1 collagen produces and promotes test
In the RPMI-1640 culture medium that contains 10%FCS (hyclone), mediator's normal fibroblast strain system (Trees stands strain) WI-38 cell is 1 * 10
5Cell/ml (cell suspending liquid) adds 100 μ l cell suspending liquids in every hole.Then, with this plate in 37 ℃, 5%CO
2Overnight incubation in-95% air, mixture with RPMI-1640 culture medium 150 μ L and PBS (-) 40 μ L carries out the culture medium exchange, in each hole, add the pollen load extract of embodiment 1 and 2 preparations, in the 5%CO2-95% air, cultivating 48 hours under 37 ℃.With 1500rpm centrifugalize gained culture, obtain supernatant then.
(1) preparation of test sample
(1-1) pepsin of the collagen in the culture supernatant
(i) dissolving pepsin (MIKUNI chemical industry (strain) system) is in the aqueous acetic acid (Northeast chemistry (strain) system) of 150mM, and making its concentration is 0.6mg/mL.
(ii) in 0.2mL cultured cell supernatant, add the above-mentioned pepsin solution of 0.1mL, utilize to put upside down to mix fully and stir.
(iii) place a night down for 4 ℃.
(iv) add and contain the 200mM Tris of 0.1mL and the aqueous solution of 150mM NaCl neutralizes, put upside down to mix fully and stir, with gained liquid as the mensuration collagen solution.
(1-2) as the pepsin of the sedimentary collagen of extracellular matrix
(i) dissolving pepsin (MIKUNI chemical industry (strain) system) is in the aqueous acetic acid (Northeast chemistry (strain) system) of 50mM, and making its concentration is 0.1mg/mL.
After (ii) from culture dish, removing culture fluid, peel cell, in culture dish, add pepsin solution with culture fluid equivalent with the microcell curette.
(iii) 4 ℃ vibrate a night down.
Supernatant is reclaimed in (iv) centrifugalize 10 minutes under 10000 * g.
(v) add the aqueous solutions that contain 200mM Tris and 150mM NaCl that institute adds 1/3rd amounts of pepsin solution, utilize to put upside down and mix fully stirring, with gained liquid as the mensuration collagen solution.
(1-3) preparation of test sample
Mixing by above-mentioned (1-1) and (1-2) preparation mensuration obtain working sample with collagen solution.
(2) measure
As object, end user's collagen type 1ELISA test kit (ACBio corporate system) is measured collagen and is produced the promotion rate with the working sample that made by said method.Assay method is according to the operation instructions of test kit.The result is as shown in table 4.
In addition, the collagen quantity under the situation of each extract that adds 10 μ g water replacement pollen load is set at 100%, calculates collagen and produce promotion rate (increment rate).
Table 4
Collagen produces and promotes result of the test
| Sample | Sample (μ g/ml) | Collagen concentration (μ g/ml) | Increment rate (%) |
| Frostweed pollen load water extract | 100 | 0.301 | 461 |
| Frostweed pollen load ethanol extraction | 100 | 0.413 | 632 |
| Flos Camelliae Japonicae powder ball extract | 100 | 0.444 | 680 |
| Flos Camelliae Japonicae powder ball ethanol extraction | 200 | 1.065 | 1630 |
| The Pollen Brassicae campestris water extract | 100 | 0.927 | 1419 |
Test example 2 hyaluronic acids produce and promote test
In the RPMI-1640 culture medium that contains 10%FCS (hyclone), mediator's normal fibroblast strain system (Trees stands strain) WI-38 cell is 1 * 10
5Cell/ml (cell suspending liquid) adds 100 μ l cell suspending liquids in every hole.Then, with this plate in 37 ℃, 5%CO
2Overnight incubation in-95% air, mixture with RPMI-1640 culture medium 150 μ L and PBS (-) 40 μ L carries out the culture medium exchange, in each hole, add the pollen load extract of embodiment 1 and 2 preparations, in the 5%CO2-95% air, cultivating 48 hours under 37 ℃.Then, centrifugalize gained culture is 15 minutes under 1500rpm, obtains supernatant.Use commercially available hyaluronic acid to measure test kit (Hyaluronan Assay Kit: biochemical industry (strain) system) measure the hyaluronic acid amount in the gained supernatant.
Hyaluronic acid amount under the situation of each extract that adds 10 μ g water replacement pollen load is set at 100%, calculates hyaluronic acid and produce promotion rate (increment rate).The result is as shown in table 5.
Table 5
Hyaluronic acid produces and promotes result of the test
| Sample | Sample (μ g/ml) | Collagen concentration (μ g/ml) | Increment rate (%) |
| Rape flower powder ball water extract | 100 | 0.361 | 131 |
| Buckwheat Flower powder ball ethanol extraction | 100 | 0.392 | 143 |
By The above results as can be known, as the pollen load extract of object of the present invention, have good collagen and produce facilitation or hyaluronic acid generation facilitation.Particularly clearly known, the collagen of rape flower powder ball water extract produces facilitation and hyaluronic acid generation facilitation is all very strong, and is useful as cosmetic material and food material with various effects such as beautifying skin effect, aging resistance effect, whitening function, moisture-keeping function.
Formulation Example 1: astringent
Use each composition as shown in table 6 below, make astringent according to well-established law.
Table 6
| Composition | Composite rate (weight %) |
| Ethanol | 5.0 |
| Glycerol | 4.0 |
| 1,3 butylene glycol | 4.0 |
| Rape flower powder ball water extract | 0.5 |
| Citric acid | In right amount |
| Sodium citrate | In right amount |
| Purified water | Remaining |
Formulation Example 2: beautifying liquid
Use each composition as shown in table 7 below, make beautifying liquid according to well-established law.
Table 7
| Composition | Composite rate (weight %) |
| Xanthan gum | 0.4 |
| Hydroxyethyl-cellulose | 0.1 |
| CVP Carbopol ETD2050 | 0.1 |
| 1,3 butylene glycol | 5.0 |
| Potassium hydroxide | 2.5 |
| Ethanol | 3.0 |
| Rape flower powder ball water extract | 1.0 |
| Purified water | Remaining |
Formulation Example 3: emulsion
Use each composition as shown in table 8 below, make emulsion according to well-established law.
Table 8
| Composition | Composite rate (weight %) |
| Squalane | 12.0 |
| CVP Carbopol ETD2050 | 0.1 |
| Xanthan gum | 0.2 |
| Triethanolamine | 0.1 |
| 1,3 butylene glycol | 5.0 |
| Rape flower powder ball water extract | 1.0 |
| Purified water | Remaining |
Claims (7)
1. the generation promoter of at least one in collagen or the hyaluronic acid is characterized in that, comprises to be selected from least a in the group of being made up of pollen, pollen load and their extract.
2. generation promoter according to claim 1, wherein, described pollen or pollen load are from belonging to the plant that is selected from least one section in the group of being made up of Cruciferae, Polygonaceae, Theaceae and Cistaceae.
3. generation promoter according to claim 2, wherein, described plant is to be selected from least a in the group of being made up of frostweed, tea, Brassica campestris L and Semen Fagopyri Esculenti.
4. generation promoter according to claim 1, wherein, described extract is the extract that is selected from water, ethanol or their mixture of the pollen of at least a plant in the group of being made up of frostweed, tea, Brassica campestris L and Semen Fagopyri Esculenti or pollen load.
5. a skin preparations for extenal use wherein is mixed with each described generation inhibitor in the claim 1～4.
6. a food wherein is mixed with the generation inhibitor that contains the extract described in the claim 4.
7. food according to claim 6, it is for promoting the functional food of the generation of at least one in collagen or the hyaluronic acid.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102477410A (en) * | 2010-11-30 | 2012-05-30 | 株式会社爱茉莉太平洋 | Composition containing bifidobacterium extract for stem cell performance increasing of fat source stem cell and for skin cell proliferation |
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2007
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102477410A (en) * | 2010-11-30 | 2012-05-30 | 株式会社爱茉莉太平洋 | Composition containing bifidobacterium extract for stem cell performance increasing of fat source stem cell and for skin cell proliferation |
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