CN101177700B - 生产具有预定的翻译后修饰的蛋白质的方法和手段 - Google Patents

生产具有预定的翻译后修饰的蛋白质的方法和手段 Download PDF

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CN101177700B
CN101177700B CN2007101494988A CN200710149498A CN101177700B CN 101177700 B CN101177700 B CN 101177700B CN 2007101494988 A CN2007101494988 A CN 2007101494988A CN 200710149498 A CN200710149498 A CN 200710149498A CN 101177700 B CN101177700 B CN 101177700B
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epo
cell
per
protein
molecule
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CN101177700A (zh
Inventor
迪尔克·扬·埃尔贝特斯·奥普斯泰尔滕
约翰·克里斯蒂安·卡普坦
彼德鲁斯·克里斯蒂安纳斯·约翰内斯·约瑟夫斯·帕西耶
罗纳德·亨德里克·彼得·布鲁斯
亚伯拉罕·布特
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Janssen Vaccines and Prevention BV
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Crucell Holand BV
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Priority claimed from PCT/NL2002/000257 external-priority patent/WO2003089468A1/en
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    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/005—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from viruses
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/505—Erythropoietin [EPO]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10322—New viral proteins or individual genes, new structural or functional aspects of known viral proteins or genes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
    • C12N2710/00011—Details
    • C12N2710/10011—Adenoviridae
    • C12N2710/10311—Mastadenovirus, e.g. human or simian adenoviruses
    • C12N2710/10341—Use of virus, viral particle or viral elements as a vector
    • C12N2710/10343—Use of virus, viral particle or viral elements as a vector viral genome or elements thereof as genetic vector

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Hematology (AREA)
  • Physics & Mathematics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Food Science & Technology (AREA)
  • Toxicology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Virology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
CN2007101494988A 2001-10-29 2002-10-29 生产具有预定的翻译后修饰的蛋白质的方法和手段 Expired - Fee Related CN101177700B (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
NLPCT/NL01/00792 2001-10-29
PCT/NL2001/000792 WO2003050286A1 (en) 2001-10-29 2001-10-29 Methods and means for producing proteins with predetermined post-translational modifications
PCT/NL2002/000257 WO2003089468A1 (en) 2002-04-19 2002-04-19 Methods and means for producing proteins with predetermined post-translational modifications
NLPCT/NL02/00257 2002-04-19

Related Parent Applications (1)

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CNB028216903A Division CN100347306C (zh) 2001-10-29 2002-10-29 生产具有预定的翻译后修饰的蛋白质的方法和手段

Publications (2)

Publication Number Publication Date
CN101177700A CN101177700A (zh) 2008-05-14
CN101177700B true CN101177700B (zh) 2012-03-21

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CN2007101494988A Expired - Fee Related CN101177700B (zh) 2001-10-29 2002-10-29 生产具有预定的翻译后修饰的蛋白质的方法和手段

Country Status (7)

Country Link
KR (3) KR100692784B1 (ko)
CN (1) CN101177700B (ko)
AU (1) AU2002224199A1 (ko)
IL (1) IL161674A0 (ko)
MX (1) MXPA04003940A (ko)
WO (1) WO2003050286A1 (ko)
ZA (1) ZA200403209B (ko)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9244235B2 (en) 2008-10-17 2016-01-26 Foro Energy, Inc. Systems and assemblies for transferring high power laser energy through a rotating junction
TWI532495B (zh) * 2009-10-05 2016-05-11 菲瑞茵國際中心股份有限公司 藥學製劑
CN102818896B (zh) * 2011-06-09 2014-12-17 北京华大蛋白质研发中心有限公司 一种基于特异抗体的硝基化修饰位点的检测方法及特异识别scot硝基化位点的抗体
EP3382014A1 (en) * 2017-03-29 2018-10-03 CEVEC Pharmaceuticals GmbH Recombinant glycoproteins with reduced antennary fucosylation
US20230331798A1 (en) * 2020-09-22 2023-10-19 Jecho Laboratories, Inc. Glycosylation-modified erythopoietin and use thereof

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5789247A (en) * 1994-04-01 1998-08-04 Ballay; Annick Expression in non-tumoral human lymphoblastoid lines with an integrative vector
WO1998044141A2 (en) * 1997-03-27 1998-10-08 The University Of British Columbia Insect expression vectors
WO2000063403A2 (en) * 1999-04-15 2000-10-26 Crucell Holland B.V. Recombinant protein production in a human cell using sequences encoding adenovirus e1 protein

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5789247A (en) * 1994-04-01 1998-08-04 Ballay; Annick Expression in non-tumoral human lymphoblastoid lines with an integrative vector
WO1998044141A2 (en) * 1997-03-27 1998-10-08 The University Of British Columbia Insect expression vectors
WO2000063403A2 (en) * 1999-04-15 2000-10-26 Crucell Holland B.V. Recombinant protein production in a human cell using sequences encoding adenovirus e1 protein

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
John E. Cronan.Biotination of Proteins in vivo.The Journal of Biological Chemistry265 18.1990,265(8),全文.
John E. Cronan.Biotination of Proteins in vivo.The Journal of Biological Chemistry265 18.1990,265(8),全文. *
John H. Pazur et al.Oligosaccharides as Immunodeterminants of Erythropoietin forTwo Sets of Anti-Carbohydrate Antibodies.Journal of Protein Chemistry19 8.2000,265(8),全文.
John H. Pazur et al.Oligosaccharides as Immunodeterminants of Erythropoietin forTwo Sets of Anti-Carbohydrate Antibodies.Journal of Protein Chemistry19 8.2000,265(8),全文. *

Also Published As

Publication number Publication date
KR20060057648A (ko) 2006-05-26
KR100692784B1 (ko) 2007-03-12
KR100737639B1 (ko) 2007-07-09
AU2002224199A1 (en) 2003-06-23
MXPA04003940A (es) 2004-11-29
IL161674A0 (en) 2004-09-27
ZA200403209B (en) 2005-01-14
WO2003050286A1 (en) 2003-06-19
KR20050040824A (ko) 2005-05-03
CN101177700A (zh) 2008-05-14
KR100602772B1 (ko) 2006-07-20
KR20060057647A (ko) 2006-05-26

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