CN101176472B - Biological source agricultural bactericide and uses thereof - Google Patents
Biological source agricultural bactericide and uses thereof Download PDFInfo
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- CN101176472B CN101176472B CN2007101570286A CN200710157028A CN101176472B CN 101176472 B CN101176472 B CN 101176472B CN 2007101570286 A CN2007101570286 A CN 2007101570286A CN 200710157028 A CN200710157028 A CN 200710157028A CN 101176472 B CN101176472 B CN 101176472B
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Abstract
The invention relates to a biogenic agricultural fungicide and the application, belonging to the pesticide formulation and the application technical field. The invention is characterized in containing 5%-60% of biomass gasification tar, which is bamboo tar or straw tar. As a biogenic agricultural fungicide, the invention has advantages of good agriculture fungicidal activities, good pesticide result, as well as high control result. With the biomass gasification tar coming from nature biomass material, the produced agricultural fungicide is highly environmental friendly and plays a great important role in the safe production of agriculture products. At the same time, the utilization of biogenic agricultural fungicide belongs to the waste re-utilization of low cost, and the consumption cost is about 0.2 RMB per mu, far lower than the existing agricultural fungicide of same kind, enabling the invention to facilitate the product industrialization and the market promotion.
Description
Technical field
The invention belongs to pesticidal preparations and uses thereof technical field, be specially kind of biological source agricultural bactericide and uses thereof.
Background technology
Biomass gasification tar is the pitchy oily mater that is condensed at normal temperatures by the gas that collection after vegetable material and the industrial wood waste pyrolysis thereof obtains, mainly contain bamboo tar, wood tar and careless tar at present, bamboo tar is the accessory substance that bamboo surplus material of processing obtains through high temperature pyrolysis, wood tar is the accessory substance that wood machining residues and shell obtain through high temperature pyrolysis, and careless tar is meant the accessory substance that herbaceous plant, crop stalk and crops husk obtain through high temperature pyrolysis.Be processed in the process of corresponding bamboo charcoal, charcoal and the peat composed of rotten mosses at bamboo wood, timber and straw, owing to adopt modern machine to carry out High-efficient Production, accessory substance-the tar of Chan Shenging in process of production, never find its purposes and become refuse, be difficult to again carry out environmental protection treatment, so become the technical barrier of modern production charcoal.Simultaneously, closely for many years, biopesticide is developed to has market-oriented kind seldom, does not satisfy the needs of the production of modern green and organic farm products far away, especially bactericide.
Application number: 00117725.7, name is called: the patent application document of using the production method of preparing tar-acetic and composite emulsion using timber dry distilling, disclose the main technique flow process: timber is pulverized drying-destructive distillation-separation dry distillation liquid, obtain therefrom that wood tar and pyroligneous liquor-medicine processes raw material, promptly wood tar is carried out emulsification, pyroligneous liquor is carried out oxidation-proportioning mixes, promptly choose various medicaments in proportion and mix-finished product according to the requirement of product.Application number: 00117726.5, name is called: the application documents of tar-acetic acid composite emulsion as multipurpose pesticide, disclose coal tar and wood tar former medicine composition as agricultural chemicals, use acetic acid as synergist, with ethanol as solvent, coal tar and wood tar are emulsified into the missible oil manufacturing and agricultural chemicals, its purposes for suppress virus, kill mould, coordinate plant growth grows.Yet the product that adopts above-mentioned disclosed technical scheme to make mainly is to suppress virus and kill mould, does not study and tests at serious fungal disease takes place on the agricultural.
Summary of the invention
For overcoming problems of the prior art, the object of the present invention is to provide the technical scheme of a kind of biological source agricultural bactericide and uses thereof, this bactericide production cost is low, bactericidal effect good and little to environmental influence.
Described a kind of biological source agricultural bactericide is characterized in that containing biomass gasification tar 15-60%, and described biomass gasification tar is bamboo tar or careless tar.
Described a kind of biological source agricultural bactericide is characterized in that containing biomass gasification tar 20-55%.
Described a kind of biological source agricultural bactericide is characterized in that containing biomass gasification tar 25-50%.
Described a kind of biological source agricultural bactericide, it is characterized in that containing biomass gasification tar 15-28%, emulsifier 25-35%, surplus is an organic solvent, the formulation of making is a soluble liquid, described emulsifier is a farm chemical emulgent 602, and described organic solvent is the mixed solvent of dimethyl formamide and absolute ethyl alcohol.
Described a kind of biological source agricultural bactericide, it is characterized in that containing biomass gasification tar 29-60%, emulsifier 15-40%, surplus is an organic solvent, the formulation of making is a cream preparation, described emulsifier is the blended emulsifier of OP-10, calcium dodecyl benzene sulfonate and dimethyl formamide, and described organic solvent is the dimethyl methyl amide.
Described kind of biological source agricultural bactericide is characterized in that biomass gasification tar is bamboo tar or careless tar.
Described a kind of biological source agricultural bactericide is characterized in that containing biomass gasification tar 24-26%, emulsifier 28-32%, and surplus is that solvent can be made the water-soluble liquid preparation.
Described a kind of biological source agricultural bactericide is characterized in that containing biomass gasification tar 30-60%, emulsifier 25-40%, and surplus is that solvent can be made cream preparation.
Described a kind of biological source agricultural bactericide is prevented and treated early blight of tomato (Alternaria Solani) in preparation, pear scab (pear scab, venturia nashicola), rice sheath blight disease rice sheath blight (Rhizoctonia solani K ü hn), corn southern leaf blight (Bipolaris maydis, Cochliobolusheterostrophus), the corn northern leaf blight (Exsero turcicum, Trichometasphaeriaturcical), cotton wilt (Fsarium oxysporum f.sp.vasinfectum (Atk.) Synderand Hanen), cucumber fusarium axysporum (Cucumber Fusarium Wilt), graw mold of tomato (tomato graymold), cucumber anthracnose (Cucumber anthracnose), wheat scab (Wheat Breeding), apple canker (Valsa mali Miyabe et Yamada), anthracnose (Anthracnose), rice blast (riceblast), Brown Spot of Pine Needles (Brown spot needle blight fungus), powdery mildew of cucumber (Cucumberpowdery mildew), barley head blight (Fusarium Head Blight in Barley), sclerotinia rot of colza (rape sclertiniose), Alternaria (Alternaria sp.), rhizoctonia (Rhizoctonia solaniKuhn), stem rot of cucumber (Cucumber Sclerotinia rot), anthracnose of grape (Elsinoe ampelina (De B.) Shear.), Botrytis cinerea (Botrytis cinerea), sickle-like bacteria (Fusarium), application in rice green smut (Rice False Smut) or rice sheath blight disease (the Rhizoctonia solani K ü hn) agricultural chemicals.
Described a kind of biological source agricultural bactericide can be mixed with formulations such as missible oil, soluble liquid, wetting powder and sustained release agent.
Above-mentioned a kind of biological source agricultural bactericide has good agriculture bactericidal activity, good drug efficacy, anti-efficient height; Because biomass gasification tar derives from the natural biological material, disinfectant use in agriculture that makes and Environmental compatibility are good, and the safety in production of agricultural product is had important effect; Simultaneously, use biomass gasification tar to belong to twice laid, it is cheap, and use cost is about 0.2 yuan/mu, far below existing similar disinfectant use in agriculture, and the industrialization and the marketization easily.
Percentage composition is the weight percentage of pure material in the present specification.
Embodiment
Now, further specify beneficial effect of the present invention in conjunction with the test of pesticide effectiveness of the present invention.
Embodiment 1: input 180kg emulsifier op-10,120kg emulsifier calcium dodecyl benzene sulfonate, 12kg dimethyl formamide are stirred into compound emulsifying agent in the 500L reactor, and be standby; In the 1000L reactor, drop into bamboo tar or careless tar 250kg, dimethyl formamide 150kg and stir, after whole dissolvings, slowly drop into the standby compound emulsifying agent of 100kg again, stir, finished product 50% bamboo tar or careless tar missible oil.
Embodiment 2: drop into dimethyl formamide 200kg, absolute ethyl alcohol 250kg in the 1500L reactor, constantly slowly drop into 250kg bamboo tar or careless tar under the condition of stirring after dissolving fully, drop into 300kg farm chemical emulgent 602 again, and fully stir, final products 25% bamboo tar or careless tar soluble liquid.
Embodiment 3: input 180kg emulsifier op-10,120kg emulsifier calcium dodecyl benzene sulfonate, 12kg dimethyl formamide are stirred into compound emulsifying agent in the 500L reactor, and be standby; In the 1000L reactor, drop into careless tar or bamboo tar 200kg, dimethyl formamide 150kg and stir, after whole dissolvings, slowly drop into the standby compound emulsifying agent of 150kg again, stir, finished product 40% careless tar or bamboo tar missible oil.
The control efficiency of above-mentioned disinfectant use in agriculture below is described by test.
1. rice banded sclerotial blight:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.1205X+1.8804 | ?0.890 | ?0.047 | ?10.58998664 |
| Embodiment 2 | ?Y＝0.1361X+1.9777 | ?0.967 | ?0.017 | ?19.26043143 |
| Embodiment 3 | ?Y＝0.1351X+1.9233 | ?0.995 | ?0.002 | ?26.58511351 |
2. barley is red mould:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.5897X+5.9811 | ?0.964 | ?0.018 | ?91.90766447 |
| Embodiment 2 | ?Y＝0.1331X+1.8654 | ?0.939 | ?0.007 | ?35.05990839 |
| Embodiment 3 | ?Y＝0.1597X+1.9516 | ?0.967 | ?0.003 | ?112.8396365 |
3. apple decay:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.1401X+2.0608 | ?0.999 | ?0.001 | ?14.51085 |
| Embodiment 2 | ?Y＝0.1173X+1.9089 | ?0.981 | ?0.001 | ?6.076493873 |
| Embodiment 3 | ?Y＝0.1112X+1.8245 | ?0.953 | ?0.004 | ?6.716312944 |
4. gibberella saubinetii:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.2970X+3.0705 | ?0.996 | ?0.002 | ?174.2739355 |
| Embodiment 2 | ?Y＝0.3299X+3.2242 | ?0.965 | ?0.003 | ?259.2665605 |
| Embodiment 3 | ?Y＝0.2134X+2.3408 | ?0.950 | ?0.005 | ?179.3709845 |
5. rape sclerotium:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.1908X+2.3129 | ?0.964 | ?0.003 | ?74.73423 |
| Embodiment 2 | ?Y＝0.2044X+2.2747 | ?0.969 | ?0.002 | ?169.5292 |
| Embodiment 3 | ?Y＝0.1718X+2.0049 | ?0.942 | ?0.006 | ?156.9467172 |
6. anthrax:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.5434X+4.9195 | ?0.904 | ?0.013 | ?293.6708 |
| Embodiment 2 | ?Y＝0.3066X+3.1323 | ?0.991 | ?0.005 | ?186.8035 |
| Embodiment 3 | ?Y＝0.2090X+2.3724 | ?0.978 | ?0.011 | ?128.5938365 |
7. paddy rice rice blast:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.2459X+2.9553 | ?0.858 | ?0.074 | ?46.08821719 |
| Embodiment 2 | ?Y＝0.1604X+2.0501 | ?0.959 | ?0.021 | ?63.53211148 |
| Embodiment 3 | ?Y＝0.1555X+1.9136 | ?0.919 | ?0.041 | ?112.7119351 |
8. the big spot of corn:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.2359X+2.5576 | ?0.943 | ?0.006 | ?162.9055 |
| Embodiment 2 | ?Y＝0.2220X+2.3623 | ?0.950 | ?0.005 | ?227.4139 |
| Embodiment 3 | ?Y＝0.2419X+2.5322 | ?0.936 | ?0.033 | ?224.6443337 |
9. tomato epidemic disease early:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.2820X+3.0512 | ?0.887 | ?0.058 | ?117.7663 |
| Embodiment 2 | ?Y＝0.2204X+2.3832 | ?0.902 | ?0.013 | ?194.6194 |
| Embodiment 3 | ?Y＝0.3567X+3.5007 | ?0.847 | ?0.256 | ?222.0981 |
10. cucumber anthrax:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.4055X+4.0140 | ?0.966 | ?0.017 | ?172.3739 |
| Embodiment 2 | ?Y＝0.2103X+2.3210 | ?0.987 | ?0.001 | ?173.5476 |
| Embodiment 3 | ?Y＝0.2088X+2.2794 | ?0.966 | ?0.003 | ?199.0348 |
11. cucumber is withered:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.2074X+2.3776 | ?0.896 | ?0.004 | ?117.035 |
| Embodiment 2 | ?Y＝0.2103X+2.2899 | ?0.942 | ?0.001 | ?201.2074 |
| Embodiment 3 | ?Y＝0.2247X+2.4154 | ?0.963 | ?0.003 | ?198.5927 |
12. cotton is withered:
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.2800X+2.9817 | ?0.952 | ?0.001 | ?141.4995 |
| Embodiment 2 | ?Y＝0.2350X+2.5352 | ?0.956 | ?0.001 | ?173.3105 |
| Embodiment 3 | ?Y＝0.2293X+2.4642 | ?0.965 | ?0.001 | ?190.4596 |
13. tomato gray mould
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.1846X+2.4879 | ?0.897 | ?0.053 | ?21.0483 |
| Embodiment 2 | ?Y＝0.1995X+2.4436 | ?0.844 | ?0.081 | ?58.7420 |
| Embodiment 3 | ?Y＝0.2120X+2.4705 | ?0.879 | ?0.019 | ?91.8998 |
14. pears are deceived star
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.2574X+2.9838 | ?0.983 | ?0.009 | ?64.45311 |
| Embodiment 2 | ?Y＝0.2782X+2.8663 | ?0.908 | ?0.003 | ?202.3015 |
| Embodiment 3 | ?Y＝0.2556X+2.6342 | ?0.908 | ?0.003 | ?236.452 |
15. pine needle foxiness
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.3?154X+3.6027 | ?0.934 | ?0.033 | ?53.4187 |
| Embodiment 2 | ?Y＝0.1900X+2.2690 | ?0.938 | ?0.007 | ?90.4669 |
| Embodiment 3 | ?Y＝0.2299X+2.5137 | ?0.977 | ?0.001 | ?157.0375 |
16. interlinkage spore
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.2521X+2.8683 | ?0.977 | ?0.001 | ?83.19794 |
| Embodiment 2 | ?Y＝0.2650X+2.7526 | ?0.949 | ?0.005 | ?203.3916 |
| Embodiment 3 | ?Y＝0.3266X+3.1247 | ?0.980 | ?0.010 | ?323.4597 |
17. rhizoctonia
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.1294X+1.9901 | ?0.951 | ?0.025 | ?9.974727 |
| Embodiment 2 | ?Y＝0.1198X+1.8292 | ?0.960 | ?0.020 | ?15.1857 |
| Embodiment 3 | ?Y＝0.2268X+2.5428 | ?0.905 | ?0.013 | ?122.5423 |
18. cucumber sclerotium
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.1074X+1.7317 | ?0.946 | ?0.098 | ?10.45592 |
| Embodiment 2 | ?Y＝0.2570X+2.7183 | ?0.931 | ?0.035 | ?178.3937 |
| Embodiment 3 | ?Y＝0.1004X+1.4037 | ?0.997 | ?0.037 | ?123.2869 |
19. grape hemorrhagic black smallpox
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.1826X+2.0717 | ?0.956 | ?0.004 | ?182.7597 |
| Embodiment 2 | ?Y＝0.1977X+2.1364 | ?0.976 | ?0.002 | ?254.2512 |
| Embodiment 3 | ?Y＝0.1874X+2.0511 | ?0.967 | ?0.003 | ?254.3123 |
20. Botrytis cinerea
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.2587X+2.7224 | ?0.922 | ?0.009 | ?185.8361 |
| Embodiment 2 | ?Y＝0.2519X+2.6756 | ?0.959 | ?0.001 | ?177.4608 |
| Embodiment 3 | ?Y＝0.2369X+2.5695 | ?0.950 | ?0.001 | ?160.735 |
21. sickle-like bacteria
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.1373X+1.9067 | ?0.960 | ?0.001 | ?35.5188 |
| Embodiment 2 | ?Y＝0.2755X+2.8262 | ?0.968 | ?0.000 | ?215.283 |
| Embodiment 3 | ?Y＝0.2333X+2.4143 | ?0.931 | ?0.002 | ?273.1976 |
22. corn stigma
| Preparation | Regression equation | The Rsp correlation coefficient | Sigf significance level | ?LC 50(effective tar content, mg/L) |
| Embodiment 1 | ?Y＝0.2150X+2.4170 | ?0.948 | ?0.005 | ?134.1866 |
| Embodiment 2 | ?Y＝0.1003X+1.3200 | ?0.992 | ?0.004 | ?281.4731 |
| Embodiment 3 | ?Y＝0.3416X+3.2384 | ?0.904 | ?0.200 | ?330.0081 |
One. prevent and treat the powdery mildew of cucumber field control effectiveness test
1. material and method
1.1 reagent agent
Embodiment 1-3 (Zhejiang Forestry Institute provides)
1.2 contrast medicament
0.3% polyoxin AS (trade name: the standing grain health, section's biotechnology Co., Ltd produces in the ShenYang, Liaoning Province)
1.3 trial crops and controlling object
Trial crops cucumber, kind are local white Beijing opera melon, and controlling object is a powdery mildew of cucumber.
1.4 experimental scheme and method
1.4.1 field design
6 processing are established in test altogether, are respectively: 500 times of embodiment 1-3,800 times of 0.3% polyoxin AS and clear water blank repeat sub-district area 10m 3 times
2, district's group randomized arrangement is not established guard row all around, encloses the film protection during dispenser.
1.4.2 overview experimental field
The test previous crops is a winter booth celery, and soil property is a sandy loam, pH value 7.2, and the content of organic matter 3.1%, fertility is higher, the whole about 200m of canopy area
2, sowing on November 14th, 2007,14-15 day field planting in January, the cucumber seedling growing way is tested and was not used any bactericide in preceding 20 days than balance.
1.4.3 spraying time and method
Test in medication on May 31 in 2007 once, this moment, cucumber was prosperous fruit latter stage breeding time, and powdery mildew of cucumber extremely sporadicly takes place, because morbidity is few at that time, so several sick leaves of the preceding excision of dispenser.Dispenser adopts workers and peasants-16 type manual sprayer carefully to spray every 667m
2Be 100 kilograms with amount of liquid medicine.
1.4.4 investigation content and method
Tested behind medicine 7 days, every sub-district random sampling 2 points, every continuous 4 strains of investigation, totally 8 strains, the whole blades of every strain are investigated in classification, calculate disease index and preventive effect, and carry out the new multipole of Deng Kenshi poor (DMRT) significance analysis, grade scale and computational methods are carried out according to the GB/T17980.30-2000 regulation.
Also in the influence situation of each investigation day range estimation dispenser to cucumber growth, life is investigated to safety in test.
2. result of the test
2.1 control efficiency to powdery mildew of cucumber
As can be seen from Table 1,500 times of embodiment 1-3,7 days average preventive effects to powdery mildew of cucumber are respectively 78.0%, 71.7% behind the medicine, 63.6%, 800 times of preventive effects of contrast medicament 0.3% polyoxin AS are 52.2%, through (DMRT) significance analysis, 500 of embodiment 1-2 show work be better than 800 times of 0.3% polyoxin AS,
2 500 times of embodiment and 800 times of indifference significancees of 0.3% polyoxin AS.
2.2 safety is given birth to: each chemicals treatment of range estimation all has no effect to cucumber behind the medicine.
3. brief summary
3.1 result of the test shows: under this experimental condition, embodiment 1,2 bactericide are better than 800 times of processing of registered biological bactericide 0.3% polyoxin AS of using on the current market to the average preventive effect of powdery mildew of cucumber under the situation of 500 times of working concentrations; Embodiment 3 bactericide and 500 times are suitable with 800 times of preventive effects of 0.3% polyoxin AS.Therefore, see that from this test the embodiment 1-3 bactericide that Zhejiang Forestry Institute provides has good control efficiency to powdery mildew of cucumber, have application value preferably on producing, general working concentration is 500-800 times.
Table 1 embodiment 1-3 prevents and treats powdery mildew of cucumber test of pesticide effectiveness table as a result
Unit: doubly, sheet, %
| Handle title | Repeat | Investigate total leaf | Classification | Disease refers to | Preventive effect | ||||
| 1 | 3 | 5 | 7 | 9 | |||||
| 1 500 times of embodiment | 1 | 198 | 8 | 1 | 13 | 4.3 | 68.4 | ||
| 2 | 164 | 8 | 9 | 3 | 1 | 3.9 | 89.6 | ||
| 3 | 160 | 9 | 15 | 13 | 8.3 | 76.1 | |||
| On average | 78.0 | ||||||||
| LSR | a?A | ||||||||
| 2 500 times of embodiment | 1 | 236 | 46 | 19 | 5 | 6.0 | 55.4 | ||
| 2 | 164 | 10 | 5 | 2 | 1 | 2 | 4.1 | 89.0 | |
| 3 | 160 | 2 | 11 | 18 | 3 | 10.1 | 70.7 | ||
| On average | 71.7 | ||||||||
| LSR | a?AB | ||||||||
| 3 500 times of embodiment | 1 | 140 | 7 | 11 | 5 | 2 | 6.3 | 53.6 | |
| 2 | 143 | 23 | 14 | 11 | 2 | 1 | 11.1 | 69.9 | |
| 3 | 188 | 10 | 19 | 14 | 4 | 3 | 11.3 | 67.2 | |
| On average | 63.6 | ||||||||
| LSR | ab?AB | ||||||||
| 0.3% standing grain health AS800 doubly | 1 | 140 | 7 | 12 | 7 | 6.2 | 54.2 | ||
| 2 | 160 | 8 | 25 | 35 | 1 | 18.4 | 50.2 | ||
| 3 | 224 | 22 | 29 | 19 | 12 | 5 | 16.5 | 52.2 | |
| On average | 52.2 |
| LSR | b?B | ||||||||
| CK | 1 | 162 | 21 | 16 | 7 | 3 | 8 | 13.5 | 0.0 |
| 2 | 110 | 13 | 27 | 39 | 11 | 37.0 | 0.0 | ||
| 3 | 128 | 10 | 8 | 20 | 21 | 13 | 34.5 | 0.0 | |
| On average | 0.0 | ||||||||
| LSR | d?C |
Two, control rice green smut field control effectiveness test
1. material and method
1.1 reagent agent
Embodiment 1-3 (Zhejiang Forestry Institute provides)
1.2 contrast medicament
20% triazolone EC (Jiangsu Province Nanjing HongTaiYang Co., Ltd production, commercially available)
1.3 trial crops and controlling object
Trial crops hybrid rice, kind are " two excellent trainings nine "; Controlling object is a rice green smut.
1.4 experimental scheme and method
1.4.1 field design
Test is carried out in single cropping hybridization rice field, and 5 processing are established in test altogether, and be respectively: 500, the 20% triazolone EC1000 of embodiment 1-3 doubly reach the clear water blank, repeat sub-district area 30m 3 times
2, district's group randomized arrangement is established guard row all around.
1.4.2 overview experimental field
The test previous crops is a winter rape, and soil property is a sandy loam, pH value 6.5, the content of organic matter 2.9%, middle fertility, the whole about 650m of field area
2, sowing on May 24th, 2007, June 19 transplanted, and paddy rice seedling growing way is not used any bactericide than balance before the test.
1.4.3 spraying time and method
Test in medication on August 23 in 2007 once, growth period duration of rice this moment is booting stage, beginning August 26 fringe.Dispenser adopts workers and peasants-16 type manual sprayer carefully to spray every 667m
2Be 50 kilograms with amount of liquid medicine.
1.4.4 investigation content and method
Test paddy rice investigation in 5 days October stage of yellow ripeness behind medicine, because this year, the false smut morbidity was lighter, middle 250 clumps of every sub-district is got in this test, and investigation morbidity grain number calculates preventive effect, and carries out the new multipole of Deng Kenshi poor (DMRT) significance analysis.
Test also in the influence situation of each investigation day range estimation dispenser to paddy growth, is investigated safety.
2. result of the test
2.1 control efficiency to rice green smut
As can be seen from Table 2,500 times of embodiment 1-3, average preventive effect to rice green smut is respectively 73.6%, 64.6%, 56.3%, contrast medicament 20% triazolone EC1000 times preventive effect is 59.2%, through (DMRT) significance analysis, embodiment 1 500 shows work and is better than its excess-three chemicals treatment, indifference significance between all the other chemicals treatment.
2.2 safety is given birth to: each chemicals treatment of range estimation all has no effect to paddy rice behind the medicine.
3. brief summary
Result of the test shows: under this experimental condition, embodiment 1-3 is to rice safety, under the situation of 500 times of working concentrations, to the registered bactericide 20% triazolone EC1000 that uses on the average preventive effect of rice green smut and the current market doubly quite.Therefore, 500 of bactericide embodiment 1-3 times have the better prevention effect to rice green smut as can be seen, have application value preferably on producing, general uses working concentration as 500-800 doubly, the time working concentration seriously takes place can bring up to 300 times.
The table 2 embodiment 1-3 control rice green smut test of pesticide effectiveness is table as a result
Position: times ,/250 clumps, %
| Handle title | Repeat | Investigation is from number | The infected seed number | Preventive effect |
| 1 500 times of embodiment | 1 | 250 | 29 | 74.1 |
| 2 | 250 | 51 | 67.1 | |
| 3 | 250 | 20 | 79.6 | |
| On average | 73.6 | |||
| LSR | a?A | |||
| 2 500 times of embodiment | 1 | 250 | 44 | 60.7 |
| 2 | 250 | 58 | 62.6 | |
| 3 | 250 | 29 | 70.4 | |
| On average | 64.6 | |||
| LSR | b?AB | |||
| 3 500 times of embodiment | 1 | 250 | 58 | 48.2 |
| 2 | 250 | 69 | 55.5 | |
| 3 | 250 | 34 | 65.3 | |
| On average | 56.3 | |||
| LSR | b?B | |||
| 20% triazolone EC1000 | 1 | 250 | 49 | 56.3 |
| 2 | 250 | 73 | 52.9 | |
| 3 | 250 | 31 | 68.4 | |
| On average | 59.2 | |||
| LSR | b?B | |||
| CK | 1 | 250 | 112 | 0.0 |
| 2 | 250 | 155 | 0.0 | |
| 3 | 250 | 98 | 0.0 | |
| On average | 0.0 | |||
| LSR | c?C |
Annotate: significance is changed post analysis through arcsine
Three, prevent and treat the rice sheath blight disease test of pesticide effectiveness
Trial crops: paddy rice
Controlling object: rice sheath blight disease
Reagent agent: test medicine, embodiment 1-3 (Zhejiang Forestry Institute provides); The contrast medicament, 5% jinggangmeisu WC (Huifeng, Tonglu, Zhejiang biochemical industry Co., Ltd)
Method: spraying; Water consumption: 675 liters/hectare.
Sub-district area: 22M
2, number of repetition: 4 times
Application method: spraying.The HD400 knapsack sprayer that Singapore Li Nong private limited partnership produces, Fan spray head, pressure 45 handkerchiefs, injection rate 1250 ml/min.
Spraying time and number of times: on the October 3rd, 2007 of dispenser for the first time, used the medicine second time every 7 days.
Use capacity: 40kg.
Control time and number of times: investigation radix before the medicine, 7 days investigation prevention effects behind the medicine for the second time.
Investigation method: 5 samplings of every sub-district diagonal, every some investigation links to each other 5 clumps totally 25 clumps.
0 grade: complete stool is anosis;
1 grade: quaterfoil and following each leaf sheath thereof, blade morbidity (is first leaf with sword-like leave);
2 grades: the 3rd blade and following each leaf sheath thereof, blade morbidity;
3 grades: second blade and following each leaf sheath thereof, blade morbidity;
4 grades: sword-like leave sheet and following each leaf sheath thereof, blade morbidity;
5 grades: the complete stool morbidity, withered ahead of time.
The drug effect computational methods
Preventive effect %=(1-Pt/Cko) * 100; Pt: the disease of treatment region refers to increased numbers; The disease of Cko check plot refers to increased numbers.
Direct influence to crop: no poisoning.
Use optimum period: usefulness when rice sheath blight disease is just sent out, spraying; General 2 times (morbidity just with 1 time, used 1 time in 7-10 days behind the medicine) looks the state of an illness a situation arises determining that the usefulness of back is medicinal again.Dosage 500-1000 times liquid.
Safety: to rice safety.In the process of the test not the water breakthrough rice have poisoning to take place, result of the test sees Table 3-1,3-2.
Table 3-1 prevents and treats rice sheath blight disease test initial data statistical form
| Handle | Repeat | Diseased plant radix investigation results at different levels before the medicine | 7 days diseased plant investigation results at different levels behind the last medicine | Preventive effect % | ||||||||||||
| Total strain number | 1 grade | 3 grades | 5 grades | 7 grades | 9 grades | Disease refers to | Total strain number | 1 grade | 3 grades | 5 grades | 7 grades | 9 grades | Disease refers to | |||
| 1 500 times of embodiment | 1 | 176 | 27 | 3 | 0 | 0 | 0 | 2.3 | 183 | 46 | 14 | 2 | 0 | 0 | 6.0 | 65.2 |
| 2 | 178 | 32 | 4 | 0 | 0 | 0 | 2.7 | 179 | 42 | 11 | 1 | 1 | 0 | 5.4 | 79.2 | |
| 3 | 192 | 28 | 3 | 0 | 0 | 0 | 2.1 | 191 | 45 | 14 | 2 | 0 | 0 | 5.6 | 77.8 | |
| 1 1000 times of embodiment | 1 | 185 | 29 | 4 | 0 | 0 | 0 | 2.5 | 177 | 39 | 14 | 2 | 0 | 0 | 5.7 | 69.2 |
| 2 | 185 | 22 | 2 | 0 | 0 | 0 | 1.7 | 182 | 38 | 18 | 3 | 1 | 0 | 7.0 | 56.3 | |
| 3 | 172 | 27 | 6 | 0 | 0 | 0 | 2.9 | 180 | 42 | 15 | 8 | 3 | 0 | 9.1 | 73.5 | |
| 2 1000 times of embodiment | 1 | 177 | 28 | 2 | 0 | 0 | 0 | 2.1 | 178 | 47 | 17 | 2 | 1 | 0 | 7.2 | 55.3 |
| 2 | 178 | 31 | 3 | 0 | 0 | 0 | 2.5 | 186 | 51 | 15 | 4 | 1 | 0 | 7.3 | 68.9 | |
| 3 | 173 | 25 | 4 | 0 | 0 | 0 | 2.4 | 183 | 46 | 11 | 8 | 3 | 0 | 8.5 | 69.8 | |
| 3 1000 times of embodiment | 1 | 182 | 27 | 4 | 0 | 0 | 0 | 2.4 | 179 | 36 | 19 | 8 | 1 | 0 | 8.7 | 51.5 |
| 2 | 180 | 29 | 2 | 0 | 0 | 0 | 2.2 | 184 | 38 | 15 | 5 | 1 | 0 | 6.9 | 66.1 | |
| 3 | 175 | 24 | 2 | 0 | 0 | 0 | 1.9 | 188 | 41 | 18 | 9 | 2 | 0 | 9.1 | 59.7 | |
| 200 times of 5% jinggangmeisu WC | 1 | 186 | 26 | 3 | 0 | 0 | 0 | 2.1 | 182 | 35 | 11 | 3 | 1 | 0 | 5.5 | 65.1 |
| 2 | 184 | 28 | 3 | 0 | 0 | 0 | 2.2 | 180 | 39 | 12 | 6 | 0 | 0 | 6.5 | 69.4 | |
| 3 | 177 | 23 | 5 | 0 | 0 | 0 | 2.4 | 187 | 42 | 17 | 5 | 1 | 0 | 7.4 | 73.7 | |
| Clear water | 1 | 177 | 25 | 3 | 0 | 0 | 0 | 2.1 | 182 | 79 | 34 | 8 | 6 | 0 | 16.1 | |
| 2 | 177 | 25 | 2 | 0 | 0 | 0 | 1.9 | 173 | 91 | 32 | 13 | 5 | 0 | 18.4 | ||
| 3 | 177 | 26 | 1 | 0 | 0 | 0 | 1.8 | 176 | 81 | 39 | 19 | 7 | 0 | 21.6 | ||
Table 3-2 prevents and treats the rice sheath blight disease result of the test
| Handle | Average disease refers to before the medicine | Average disease refers to behind the medicine | Average preventive effect (%) | The significance of difference | |
| 5％ | 1％ | ||||
| 1 500 times of embodiment | 2.39 | 5.7 | 74.1 | a | A |
| 1 1000 times of embodiment | 2.35 | 7.3 | 66.3 | a | A |
| 2 1000 times of embodiment | 2.34 | 7.7 | 64.7 | a | A |
| 3 1000 times of embodiment | 2.15 | 8.2 | 59.1 | a | A |
| 5% jinggangmeisu WC200 doubly | 2.24 | 6.5 | 69.4 | a | A |
By a large amount of agricultural diseases being carried out the screening active ingredients test, comprise lab screening and outdoor field control effectiveness test as can be seen tar be not that all agricultural diseases are all had active function, just at wherein seldom a part of disease is effective, and in effective germ, owing to the different different active size differences that show with crop of disease species are very big, disclosed 25 kinds of diseases all are to find to prevent and treat resultful agriculture germ with this disinfectant use in agriculture through a large amount of screening tests in the present patent application file, and undocumented all is the germ that control does not have effect.
Claims (1)
1. following a kind of biological source agricultural bactericide is prevented and treated pear scab (pear scab in preparation, venturia nashicola), rice sheath blight disease (rice sheath blight (Rhizoctoniasolani K ü hn)), corn southern leaf blight (Bipolaris maydis, Cochliobolusheterostrophus), the corn northern leaf blight (Exsero turcicum, Trichometasphaeria turcical), cotton wilt (Fsarium oxysporumf.sp.vasinfectum (Atk.) Synder and Hanen), cucumber fusarium axysporum (Cucumber Fusarium Wilt), cucumber anthracnose (Cucumber anthracnose), apple canker (Valsa mali Miyabe et Yamada), rice blast (riceblast), Brown Spot of Pine Needles (Brown spot needle blight fungus), powdery mildew of cucumber (Cucumber powdery mildew), barley head blight (Fusarium HeadBlight in Barley), sclerotinia rot of colza (rape sclertiniose), Alternaria (Alternaria sp.), rhizoctonia (Rhizoctonia solani Kuhn), stem rot of cucumber (Cucumber Sclerotinia rot), anthracnose of grape (Elsinoe ampelina (DeB.) Shear.), Botrytis cinerea (Botrytis cinerea), sickle-like bacteria (Fusarium), rice green smut (Rice False Smut), application in the pesticidal preparations of rice sheath blight disease (Rhizoctonia solaniK ü hn)
Contain biomass gasification tar 20-55%, described biomass gasification tar is bamboo tar or careless tar.
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| RU2781489C1 (en) * | 2021-12-06 | 2022-10-12 | Общество с ограниченной ответственностью Научно-Технический Центр "Химинвест" | Method for stimulating the growth of barley |
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| CN104604939A (en) * | 2015-01-08 | 2015-05-13 | 馥稷生物科技发展（上海）有限公司 | Pesticide composite containing sophocarpidine |
| CN107183061A (en) * | 2017-07-07 | 2017-09-22 | 浙江农林大学 | A kind of careless tar herbicide and its manufacture method |
| CN107232232A (en) * | 2017-07-07 | 2017-10-10 | 浙江农林大学 | A kind of bamboo tar is the natural herbicide and its manufacture method of active component |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| RU2781489C1 (en) * | 2021-12-06 | 2022-10-12 | Общество с ограниченной ответственностью Научно-Технический Центр "Химинвест" | Method for stimulating the growth of barley |
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