CN101147492A - Copper sulfate and validamycin compound germicide and using method thereof - Google Patents
Copper sulfate and validamycin compound germicide and using method thereof Download PDFInfo
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- CN101147492A CN101147492A CNA200710135172XA CN200710135172A CN101147492A CN 101147492 A CN101147492 A CN 101147492A CN A200710135172X A CNA200710135172X A CN A200710135172XA CN 200710135172 A CN200710135172 A CN 200710135172A CN 101147492 A CN101147492 A CN 101147492A
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- copper sulphate
- jinggangmycin
- rice
- jinggangmeisu
- validamycin
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Abstract
The present invention relates to a compound germicide which can be used for effectively controlling crop diseases of rice sheath blight disease and green smut of rice, etc. Said compound germicide is made up by using copper sulfate and jinggangmeisu (validamycin A) as effective components according to the mixing ratio of 1:12-12:1 through a certain preparation process.
Description
Technical field
The invention belongs to technical field of pesticide, relate to a kind of copper sulphate and validamycin compound germicide and using method thereof that can be used to prevent and treat crop pests such as rice sheath blight disease, rice green smut.
Background technology
Rice sheath blight disease is the important disease of the normal property sent out on the paddy rice, on the south the Changjiang river rice district take place serious, thereby it is withered to make that with hazard rice blade, leaf sheath blade is done sth. in advance yellow, the paddy rice functional leaf be injured the back paddy rice can not normally ear from, cause ripening rate and thousand kernel weight to descend, thereby influence rice yield.Rice sheath blight disease all can be fallen ill in whole growth period duration of rice, and is the heaviest with morbidity in 7 ~ September in the Yangtze river basin.The control of China's rice sheath blight disease uses mainly that jinggangmeisu, triazolone, standing grain fringe are peaceful, rice Feng Ling etc. is in premorbid or their early stage spraying control.But single is that control efficiency is bad with the common defective of this series bactericidal agent when preventing and treating rice sheath blight disease, also less stable.All can take place because rice sheath blight disease needs only temperature in rice plant of tillering stage to the maturing stage of earing, humidity is suitable, emergence period is long, and the control efficiency of control medicament is also not ideal, so needs repeatedly, dosage sprays medicine, to keep the control effect to rice sheath blight disease.Increased agricultural basis like this, also strengthened, simultaneously also to agricultural product quality is constituted considerable influence environmental influence.
Summary of the invention
Technical problem to be solved by this invention is that a kind of copper sulphate and validamycin compound germicide and using method thereof that plant disease is had better control efficiency is provided.
The effective ingredient that comprises in copper sulphate of the present invention and the validamycin compound germicide is copper sulphate and jinggangmycin A, and the weight ratio of two kinds of compositions is 1: 12～12: 1.Above-mentioned effective ingredient can and be filled composition with conventional auxiliary agent and together is mixed with the conventional formulations of pesticide.
The method that copper sulphate of the present invention and validamycin compound germicide are used to prevent and treat rice sheath blight disease is: at the rice sheath blight disease initial stage of origination, convert 600～900 kilograms of thick mists of spray of water according to the consumption of copper sulphate in the per hectare and jinggangmycin A total amount 50～200 grams, wherein the weight ratio of copper sulphate and jinggangmycin A is 1: 12～1: 6.
The method that copper sulphate of the present invention and validamycin compound germicide are used to prevent and treat rice green smut is: before the paddy rice cut 5～7 days, convert 600～900 kilograms of thick mists of spray of water according to the consumption of copper sulphate in the per hectare and jinggangmycin A total amount 150～450 grams, wherein the weight ratio of copper sulphate and jinggangmycin A is 1: 9～12: 1.
The method that copper sulphate of the present invention and validamycin compound germicide are used to prevent and treat wheat sharp eyespot is: at the wheat sharp eyespot initial stage of origination, convert 600～900 kilograms of thick mists of spray of water according to the consumption of copper sulphate in the per hectare and jinggangmycin A total amount 50～200 grams, wherein the weight ratio of copper sulphate and jinggangmycin A is 1: 12～1: 6.
In composite bactericide of the present invention and the using method; the mechanism of action of inorganic compound copper sulphate is to contain the very strong Cu2+ of sterilizing power in the copper sulfate solution; Cu2+ can make the protein coagulating of somatic cells film; simultaneously some copper ion also can permeate somatic cells and some enzyme enzyme of SH base (contain-) and combines; influence its activity; Cu2+ can also replace the cation (K+, H+, Ca+ etc.) on the somatic cells film; changed the normal permeability of cell membrane; make the protoplasm outflow, be the non-internal-absorting protective fungicide.After the mechanism of action of the farm antibiotics jinggangmeisu among the present invention is absorbed by germ for this bactericide and conducts in its body, promptly disturb and suppress the normal growth growth of somatic cells, cause the death of thalline, its reason is the amino acid whose combination that jinggangmeisu has hindered murein (mucopeptide) on the somatic cells wall, and the structure of cell wall is damaged, can also disturb the sterol of germ synthetic, make the mycelia top produce abnormal branch.So the present invention has utilized inorganic compound copper sulphate to the therapeutic action to germ of the protective effect of crop and agricultural antibiotic.Indoor determination of activity and field control effect test through to sheath blight fungus and rice false smut prove that bactericide of the present invention and using method have good control efficiency to rice sheath blight disease, rice green smut, wheat sharp eyespot.
Embodiment
First group of embodiment of composite bactericide of the present invention and to the indoor determination of activity of sheath blight fungus:
Adopt the serial dilution flat band method to measure the antibacterial vigor of medicament to the Rhizoctonia solani Kuhn mycelial growth.With the former powder of 60% jinggangmycin A and copper sulphate respectively water be dissolved into the stoste of 10000 μ g/ml, then with copper sulphate and jinggangmycin A by the sample that was mixed into different proportionings in 12: 1,10: 1,8: 1,6: 1,4: 1, be diluted to series concentration by different extension rates with aqua sterilisa together with single agent then.Contrast is handled with aqua sterilisa.Dissolve the back and be cooled to the quantitative medicament of adding about 45 ℃ at the WA medium, and pour culture dish rapidly into, make the pastille flat board of series concentration; To on the PSA medium, cultivate 3 days sheath blight fungus colony edges in advance and get 0.4mm bacterium sheet, move on to the dull and stereotyped central authorities of pastille.Cultivated 3 days under 25 ℃ of conditions, colony diameter is measured in right-angled intersection.And calculating bacteriostasis rate.Each concentration is handled and is repeated 4 times.Calculate the antibacterial vigor regression equation and the antibacterial middle concentration (EC of medicament with concentration logarithm-bacteriostasis rate probit value method
50).Press the abundant method of Sun Yun and calculate synergic ratio, result such as table 1:
Table 1 copper sulphate, jinggangmeisu and composite sample thereof are to rice sheath blight disease toxicity regression formula and co-toxicity coefficient
| Medicament | The toxicity regression formula | EC 50 (ug/ml) | Actual measurement toxicity index ATI | Theoretical toxicity index TTI | Co-toxicity coefficient CTC |
| Copper sulphate (A) | Y＝3.6653+0.8314x | 40.3134 | 0.3481 | ||
| Jinggangmycin A (B) | Y＝5.5761+0.6754x | 0.140314 | 100 | ||
| A∶B＝1∶12 | Y＝5.6626+0.7005x | 0.113249 | 123.8987 | 92.3345 | 133.1796 |
| A∶B＝1∶10 | Y＝5.6735+0.7100x | 0.112560 | 124.6571 | 90.9407 | 137.0751 |
| A∶B＝1∶8 | Y＝5.6192+0.6936x | 0.128009 | 109.6126 | 88.9276 | 123.2605 |
| A∶B＝1∶6 | Y＝5.4642+0.5803x | 0.158487 | 88.5334 | 85.885 | 103.0837 |
| A∶B＝1∶4 | Y＝5.2935+0.5175x | 0.270960 | 51.7840 | 80.0696 | 64.6737 |
The measurement result of table 1 shows, apparently higher than copper sulphate, jinggangmycin A suppresses the EC of mycelial growth to jinggangmycin A to the inhibition effect of Rhizoctonia solani Kuhn mycelial growth
50Value only is 0.140314 μ g/ml, and copper sulphate suppresses the EC of mycelial growth
50Value only is 40.3134 μ g/ml.
Jinggangmeisu a and copper sulphate are mixed has summation action, synergistic effect and antagonism to suppressing the Rhizoctonia solani Kuhn mycelial growth; When jinggangmeisu a and copper sulphate were mixed with 4: 1, its co-toxicity coefficient was 64.6737, and its value significantly is lower than 100, illustrated that jinggangmeisu a and copper sulphate are in this ratio tangible antagonism that has been mixed; When jinggangmeisu and copper sulphate were mixed with 6: 1, its co-toxicity coefficient was 103.0837, and it is worth a little higher than 100 but less than 120, illustrated that jinggangmeisu and copper sulphate are in this ratio summation action that has been mixed; When jinggangmeisu and copper sulphate with 8: 1,10: 1,12: 1 when being mixed, its co-toxicity coefficient is respectively 133.1796,137.0751,123.2605, its value all is significantly higher than 120, illustrates that jinggangmeisu and copper sulphate are in this ratio obvious synergistic effect that has been mixed.
Second group of embodiment of composite bactericide of the present invention and to the indoor determination of activity of rice false smut:
Adopt the serial dilution flat band method to measure the antibacterial vigor of medicament to rice green smut bacterium mycelial growth.With the former powder of 60% jinggangmeisu a and copper sulphate respectively water be dissolved into the stoste of 10000 μ g/ml, then with copper sulphate and jinggangmeisu a by the sample that was mixed into different proportionings in 1: 9,1: 5,1: 1,3: 1,5: 1,7: 1,9: 1,11: 1, be diluted to series concentration by different extension rates with aqua sterilisa together with single agent then.Contrast is handled with aqua sterilisa.Dress 20ml dilution in every 50ml triangular flask, 4 bottles of every concentration.Strict sterile working during dilution.Then, every bottle adds and has cultivated standby bacterial classification 2ml, shakes 12 days (rotating speed 120r/min) of training, static cultivation 2 days 28 ℃ of waters bath with thermostatic control.Pour out mycelia, 60 ℃ of oven dry claim mycelia heavy.Calculate the antibacterial vigor regression equation and the antibacterial middle concentration (EC of medicament with concentration logarithm-bacteriostasis rate probit value method
50).Press the abundant method of Sun Yun and calculate synergic ratio, result such as table 2:
Table 2 copper sulphate, jinggangmeisu and composite sample thereof are to rice green smut toxicity regression formula and co-toxicity coefficient
| Medicament | The toxicity regression formula | EC 50 (ug/ml) | Actual measurement toxicity index ATI | Theoretical toxicity index TTI | Co-toxicity coefficient CTC |
| Copper sulphate (A) | Y＝4.8957+1.4859x | 1.1754 | 781.7253 | ||
| Jinggangmycin a (B) | Y＝3.9545+1.0854x | 9.1884 | 100 | ||
| A∶B＝1∶9 | Y＝4.1189+1.2645x | 4.9750 | 184.6915 | 185.2157 | 99.71698 |
| A∶B＝1∶5 | Y＝4.2700+1.1171x | 4.5027 | 204.0642 | 213.6209 | 95.5263 |
| A∶B＝1∶1 | Y＝4.7187+1.1675x | 1.7416 | 527.5838 | 440.8627 | 119.6708 |
| A∶B＝3∶1 | Y＝4.8505+1.1888x | 1.2000 | 765.7000 | 611.3142 | 125.2547 |
| A∶B＝5∶1 | Y＝5.0597+1.5989x | 0.9076 | 1012.3843 | 668.1044 | 151.5302 |
| A∶B＝7∶1 | Y＝5.1123+1.6032x | 0.8510 | 1079.7180 | 657.4234 | 164.2348 |
| A∶B＝9∶1 | Y＝5.1254+1.6541x | 0.8398 | 1094.1176 | 713.5528 | 153.3338 |
| A∶B＝11∶1 | Y＝5.1465+1.7026x | 0.8203 | 1120.1267 | 724.9376 | 154.5135 |
The measurement result of table 2 shows, apparently higher than copper sulphate, jinggangmycin a suppresses the EC of mycelial growth to jinggangmycin a to the inhibition effect of rice green smut bacterium mycelial growth
50Value only is 9.1884 μ g/ml, and copper sulphate suppresses the EC of mycelial growth
50Value is 1.1754 μ g/ml.
Jinggangmeisu and copper sulphate are mixed has summation action, synergistic effect and antagonism to suppressing rice green smut bacterium mycelial growth; When copper sulphate and jinggangmeisu a with 1: 9,1: 5,1: 1 when being mixed, its co-toxicity coefficient illustrates that jinggangmeisu and copper sulphate are in this ratio summation action that has been mixed between 80～120; When copper sulphate and jinggangmeisu a with 3: 1,5: 1,7: 1,9: 1,11: 1 when being mixed, its co-toxicity coefficient is all significantly greater than 120, illustrates that jinggangmeisu and copper sulphate are in this ratio obvious synergistic effect that has been mixed.
The present invention's embodiment and the field control effect such as table 3 of jinggangmeisu and copper sulphate bactericidal agent for preventing and treating rice sheath blight disease:
Table 3 18% copper sulphate jinggangmeisu WP is to the field efficacy of rice sheath blight disease
| Formulation rate (g/hm 2) | Behind the medicine 7 days | Behind the medicine 14 days | ||
| Disease refers to (%) | Preventive effect (%) | Disease refers to (%) | Preventive effect (%) | |
| 750 | 0.89 | 94.24 | 2.32 | 91.76 |
| 562.5 | 1.28 | 91.72 | 3.26 | 88.43 |
| 375 | 3.31 | 78.59 | 5.95 | 78.88 |
| 0 | 15.46 | ----- | 28.18 | ----- |
In the present embodiment, spraying time is July 12, and converting the water yield is the 600L/ hectare.Spray thick mist.Sulfur acid copper 2%, jinggangmeisu a 16%, wetting agent 10%, auxiliary agent 2% among the 18% copper sulphate jinggangmeisu WP, all the other are inserts.
The present invention's embodiment and the field control effect such as table 4 of jinggangmeisu and copper sulphate bactericidal agent for preventing and treating rice green smut:
Table 4 49% copper sulphate jinggangmeisu WP is to the field efficacy of rice green smut
| Formulation rate (g/hm 2) | Behind the medicine 30 days | Behind the medicine 45 days | ||
| Disease tassel yield (%) | Preventive effect (%) | Disease tassel yield (%) | Preventive effect (%) | |
| 750 | 0.32 | 94.64 | 0.78 | 90.90 |
| 562.5 | 0.47 | 92.14 | 0.89 | 88.69 |
| 375 | 0.89 | 85.11 | 1.41 | 82.08 |
| 0 | 5.98 | 7.87 | ||
In the present embodiment, spraying time is August 20, and converting the water yield is the 600L/ hectare.Sulfur acid copper 40% among the 49% copper sulphate jinggangmeisu WP, jinggangmycin A 9%,, wetting agent 10%, auxiliary agent 2%, all the other are inserts.
The present invention's embodiment and the field control effect such as table 5 of jinggangmeisu and copper sulphate bactericidal agent for preventing and treating wheat sharp eyespot:
Table 5 18% copper sulphate jinggangmeisu WP is to the field efficacy of rice sheath blight disease
| Formulation rate (g/hm 2) | Behind the medicine 14 days | Behind the medicine 30 days | ||
| Disease refers to % | Preventive effect % | Disease refers to % | Preventive effect % | |
| 750 | 0.31 | 96.03 | 1.30 | 92.2 |
| 562.5 | 0.47 | 93.1 | 2.60 | 84.4 |
| 375 | 0.76 | 74.1 | 3.01 | 82.0 |
| 0 | 7.81 | 15.74 | ||
In the present embodiment, spraying time is March 20, and converting the water yield is the 600L/ hectare.The spray mist.Sulfur acid copper 2%, jinggangmeisu a 16%, wetting agent 10%, auxiliary agent 2% among the 18% copper sulphate jinggangmeisu WP, all the other are inserts.
Claims (4)
1. copper sulphate and validamycin compound germicide, it is characterized in that: the effective ingredient that comprises in its composition is copper sulphate and jinggangmycin A, the mass ratio of two kinds of compositions is 1: 12～12: 1.
2. method that described copper sulphate of claim 1 and validamycin compound germicide is used to prevent and treat rice sheath blight disease, it is characterized in that: at the rice sheath blight disease initial stage of origination, convert 600～900 kilograms of thick mists of spray of water according to the consumption of copper sulphate in the per hectare and jinggangmycin A total amount 50～200 grams, wherein the weight ratio of copper sulphate and jinggangmycin A is 1: 12～1: 6.
3. method that the described copper sulphate of claim 1 and validamycin compound germicide are used to prevent and treat rice green smut, it is characterized in that: before the paddy rice cut 5～7 days, convert 600～900 kilograms of thick mists of spray of water according to the consumption of copper sulphate in the per hectare and jinggangmycin A total amount 150～450 grams, wherein the weight ratio of copper sulphate and jinggangmycin A is 1: 9～12: 1.
4. method that the described copper sulphate of claim 1 and validamycin compound germicide are used to prevent and treat wheat sharp eyespot, it is characterized in that: at the wheat sharp eyespot initial stage of origination, convert 600～900 kilograms of thick mists of spray of water according to the consumption of copper sulphate in the per hectare and jinggangmycin A total amount 50～200 grams, wherein the weight ratio of copper sulphate and jinggangmycin A is 1: 12～1: 6.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102349544A (en) * | 2011-08-16 | 2012-02-15 | 江苏东宝农药化工有限公司 | Fungicide composition containing jingangmycin and cuprous oxide and preparation method thereof |
| CN102669100A (en) * | 2012-04-20 | 2012-09-19 | 浙江海正化工股份有限公司 | Oxine-copper and validamycin compound pesticide composition and preparation method and application thereof |
| CN103467184A (en) * | 2013-08-21 | 2013-12-25 | 池州市贵池区生产力促进中心 | Insecticide-fertilizer for preventing and curing rice false smut and preparation method and spraying method thereof |
| CN104705332A (en) * | 2015-04-02 | 2015-06-17 | 郝文杰 | Multifunctional bactericidal composition and application of multifunctional bactericidal composition |
| CN111202095A (en) * | 2020-03-10 | 2020-05-29 | 广东省农业科学院植物保护研究所 | Composition for preventing and treating bacterial leaf streak of rice as well as preparation method and application thereof |
-
2007
- 2007-11-12 CN CNA200710135172XA patent/CN101147492A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102349544A (en) * | 2011-08-16 | 2012-02-15 | 江苏东宝农药化工有限公司 | Fungicide composition containing jingangmycin and cuprous oxide and preparation method thereof |
| CN102669100A (en) * | 2012-04-20 | 2012-09-19 | 浙江海正化工股份有限公司 | Oxine-copper and validamycin compound pesticide composition and preparation method and application thereof |
| CN102669100B (en) * | 2012-04-20 | 2014-02-26 | 浙江海正化工股份有限公司 | Oxine-copper and validamycin compound pesticide composition and preparation method and application thereof |
| CN103467184A (en) * | 2013-08-21 | 2013-12-25 | 池州市贵池区生产力促进中心 | Insecticide-fertilizer for preventing and curing rice false smut and preparation method and spraying method thereof |
| CN104705332A (en) * | 2015-04-02 | 2015-06-17 | 郝文杰 | Multifunctional bactericidal composition and application of multifunctional bactericidal composition |
| CN111202095A (en) * | 2020-03-10 | 2020-05-29 | 广东省农业科学院植物保护研究所 | Composition for preventing and treating bacterial leaf streak of rice as well as preparation method and application thereof |
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